FastQCFastQC Report
Thu 26 May 2016
SRR936953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708006
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133610.49338886250621306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96920.357901718090728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64860.239512024714864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78250.043.5353281
GTATCAA135700.038.8292241
ATCAACG166150.031.488953
TCAACGC168550.030.899614
TATCAAC169800.030.784082
CAACGCA170500.030.6019545
AACGCAG175100.029.8761256
ACGCAGA195350.026.3890257
CGCAGAG195400.026.1634358
GTACATG125450.025.1480561
TACATGG126850.024.2748052
GCAGAGT205750.023.9449469
GAGTACT109600.023.53575112-13
ACATGGG129300.022.6387983
GTACTTT119300.021.42005214-15
AGTACTT114950.020.68397112-13
AGAGTAC177250.020.58419210-11
CAGAGTA200150.020.18727110-11
CATGGGG56200.019.9547794
ACTTTTT142250.018.33153216-17
Produced by FastQC (version 0.11.5)