##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936953_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2708006 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.533779097978364 31.0 31.0 34.0 30.0 34.0 2 31.649437261217294 33.0 31.0 34.0 30.0 34.0 3 31.646015555356968 33.0 31.0 34.0 28.0 34.0 4 35.240644223092566 37.0 35.0 37.0 33.0 37.0 5 35.227652006679456 37.0 35.0 37.0 33.0 37.0 6 35.32608125683621 37.0 35.0 37.0 33.0 37.0 7 35.25511464893357 37.0 35.0 37.0 32.0 37.0 8 35.211138749323304 37.0 35.0 37.0 32.0 37.0 9 36.7717848483349 39.0 37.0 39.0 32.0 39.0 10-11 36.63736527910204 39.0 37.0 39.0 32.0 39.0 12-13 36.51719401655683 39.0 37.0 39.0 32.0 39.0 14-15 37.621902240984696 40.0 37.0 41.0 32.0 41.0 16-17 37.53941350203803 40.0 37.0 41.0 32.0 41.0 18-19 37.489659365599636 40.0 37.0 41.0 32.0 41.0 20-21 37.37475877084468 40.0 37.0 41.0 31.5 41.0 22-23 37.24020497000376 39.5 37.0 41.0 31.0 41.0 24-25 37.34931791140788 40.0 37.0 41.0 31.5 41.0 26-27 37.292850163552075 40.0 37.0 41.0 31.0 41.0 28-29 37.1835130719799 40.0 37.0 41.0 31.0 41.0 30-31 36.98560564489148 39.0 36.0 41.0 30.5 41.0 32-33 36.766243317038445 39.0 36.0 41.0 30.0 41.0 34-35 36.562977149976774 39.0 35.0 41.0 30.0 41.0 36-37 36.354250507569034 39.0 35.0 41.0 30.0 41.0 38-39 36.176414675595254 39.0 35.0 40.5 30.0 41.0 40-41 35.968365653547295 39.0 35.0 40.0 29.0 41.0 42-43 35.72189223362135 38.5 35.0 40.0 27.5 41.0 44-45 35.428403962177335 38.0 35.0 40.0 26.5 41.0 46-47 35.0370335220823 38.0 34.0 40.0 25.5 41.0 48-49 34.75158086798921 38.0 34.0 40.0 24.0 41.0 50-51 34.026194735166754 37.5 33.5 39.5 23.5 40.5 52-53 34.211439154861544 38.0 33.5 39.5 24.0 40.5 54-55 34.60499902880569 38.0 34.0 40.0 24.0 41.0 56-57 34.492868184191614 38.0 34.0 40.0 23.0 41.0 58-59 34.231922122772254 38.0 34.0 40.0 23.0 41.0 60-61 33.93541244000198 38.0 33.5 40.0 22.0 41.0 62-63 33.58350166137002 37.0 33.0 40.0 21.5 41.0 64-65 33.240858218187114 37.0 33.0 40.0 20.0 41.0 66-67 32.86140244888675 36.0 32.0 39.0 19.0 41.0 68-69 32.45671039872142 36.0 32.0 39.0 18.0 41.0 70-71 32.06237744672649 35.0 31.5 38.5 15.5 40.0 72-73 31.651428394176378 35.0 31.0 38.0 12.5 40.0 74-75 31.198681428327706 35.0 31.0 37.0 8.0 39.0 76-77 30.75801401474 35.0 30.5 36.5 7.0 39.0 78-79 30.327907877604407 34.5 30.0 36.0 4.5 39.0 80-81 29.877916998706798 34.0 29.5 36.0 2.0 37.5 82-83 29.449970384112888 34.0 29.0 35.0 2.0 37.0 84-85 29.047643358249573 34.0 29.0 35.0 2.0 36.5 86-87 28.685089139388907 34.0 29.0 35.0 2.0 36.0 88-89 28.314130397052296 34.0 28.5 35.0 2.0 36.0 90-91 27.90882904247627 34.0 27.0 35.0 2.0 35.5 92-93 27.50986316130762 34.0 26.5 35.0 2.0 35.0 94-95 27.058184878467774 33.0 25.5 35.0 2.0 35.0 96-97 26.50088367603321 33.0 25.0 35.0 2.0 35.0 98-99 25.797158315011117 33.0 23.5 35.0 2.0 35.0 100-101 23.232359898759455 30.0 9.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48927454936777437 1101 2 0.5240423794734674 1101 3 0.4370764918898402 1101 4 0.460233848420593 1101 5 0.5422351528783977 1101 6 0.3735169125195483 1101 7 0.42236294442879796 1101 8 0.3716044892403829 1101 9 0.3586120845259302 1101 10-11 0.552002853823744 1101 12-13 0.64282762142021 1101 14-15 0.7350575416444087 1101 16-17 0.7555471207564253 1101 18-19 0.8623303278429617 1101 20-21 0.7695945662254928 1101 22-23 0.7818915778938802 1101 24-25 0.7334753352205112 1101 26-27 0.5672213428634407 1101 28-29 0.7385104853759117 1101 30-31 0.6547836324905418 1101 32-33 0.6628022836189089 1101 34-35 0.7857362195191797 1101 36-37 0.7588544028482858 1101 38-39 0.7934155635016609 1101 40-41 0.7403160344485187 1101 42-43 0.6825561647130982 1101 44-45 0.6592827929682414 1101 46-47 0.7136890209872959 1101 48-49 0.7394432418724506 1101 50-51 0.7141247465891141 1101 52-53 0.7323603922688946 1101 54-55 0.748118716401386 1101 56-57 0.5913207636605549 1101 58-59 0.5670015667401387 1101 60-61 0.7696281802826448 1101 62-63 0.8033933723630611 1101 64-65 0.7721478020928316 1101 66-67 0.7723266437147842 1101 68-69 0.9201506367372403 1101 70-71 0.8079412731594502 1101 72-73 0.6728192778173003 1101 74-75 0.6902278524632486 1101 76-77 0.7291250280275605 1101 78-79 0.7468049289665366 1101 80-81 0.6716489782920938 1101 82-83 0.7534669386720587 1101 84-85 0.8075497736651904 1101 86-87 0.626602721233688 1101 88-89 0.7292660697838862 1101 90-91 0.7187117361036606 1101 92-93 0.7720469338134954 1101 94-95 0.9648122182189702 1101 96-97 0.8010331523421677 1101 98-99 0.5629642510608583 1101 100-101 0.7708698303201764 1102 1 0.36933098416104926 1102 2 0.39553667465196796 1102 3 0.3613679898655455 1102 4 0.33476090031606276 1102 5 0.3557451516515542 1102 6 0.29248391043391564 1102 7 0.35992029935457737 1102 8 0.33640021982558466 1102 9 0.45159944803960883 1102 10-11 0.48673478855567254 1102 12-13 0.48310488801325846 1102 14-15 0.5664101364180567 1102 16-17 0.5944570704558529 1102 18-19 0.6278413080907868 1102 20-21 0.5846123554538138 1102 22-23 0.6714566619326519 1102 24-25 0.32369003280363273 1102 26-27 0.5370195271353069 1102 28-29 0.43256704285352043 1102 30-31 0.34986828468045417 1102 32-33 0.5568545471448587 1102 34-35 0.42845061039146515 1102 36-37 0.5497990722140074 1102 38-39 0.5831094660903062 1102 40-41 0.573417494392082 1102 42-43 0.6027125872379386 1102 44-45 0.3395263213696609 1102 46-47 0.457721655019931 1102 48-49 0.4169251459332983 1102 50-51 0.41314388534509305 1102 52-53 0.528560245047693 1102 54-55 0.5353080183275338 1102 56-57 0.675423941184782 1102 58-59 0.5750097865904635 1102 60-61 0.540407839220201 1102 62-63 0.6840515738107342 1102 64-65 0.709336490597309 1102 66-67 0.555435464718343 1102 68-69 0.6637182971470068 1102 70-71 0.556965932710419 1102 72-73 0.46516254858162753 1102 74-75 0.6085341952432763 1102 76-77 0.7692753163357438 1102 78-79 0.6536378329047032 1102 80-81 0.7109079649195031 1102 82-83 0.7083062221955565 1102 84-85 0.6419197896141533 1102 86-87 0.5510034082659523 1102 88-89 0.6509253753905604 1102 90-91 0.4568812858521589 1102 92-93 0.3813439525841993 1102 94-95 0.5020923065516847 1102 96-97 0.45293169239860376 1102 98-99 0.4941966151879633 1102 100-101 0.5459575496184215 1103 1 0.10179851662858042 1103 2 0.1816006107159076 1103 3 0.1420206705182281 1103 4 0.22785114340630486 1103 5 0.2220122179186177 1103 6 0.09667971462972247 1103 7 0.1247388141730923 1103 8 0.17914081256618175 1103 9 0.09881889525905763 1103 10-11 0.20418400600489406 1103 12-13 0.3174871723955448 1103 14-15 0.32064756565548436 1103 16-17 0.3630801140788904 1103 18-19 0.4500690803185634 1103 20-21 0.31669693753838857 1103 22-23 0.43953025500623966 1103 24-25 0.20918786830146274 1103 26-27 0.3955027106184872 1103 28-29 0.40528599057247305 1103 30-31 0.36684297665514265 1103 32-33 0.4778668681571716 1103 34-35 0.2845528414936993 1103 36-37 0.6167487891637222 1103 38-39 0.5055453635261955 1103 40-41 0.46575848673307263 1103 42-43 0.45230466183001283 1103 44-45 0.5153588222021668 1103 46-47 0.5466493939476749 1103 48-49 0.557509561517719 1103 50-51 0.5524129496141654 1103 52-53 0.603427044914497 1103 54-55 0.7163103493298664 1103 56-57 0.6270806178414574 1103 58-59 0.6003927369734114 1103 60-61 0.7499732737856348 1103 62-63 0.7285487433079041 1103 64-65 0.6454420517028723 1103 66-67 0.584223343506217 1103 68-69 0.6222930557217623 1103 70-71 0.7277451534896429 1103 72-73 0.4164612498803315 1103 74-75 0.6673337290428094 1103 76-77 0.499703221763653 1103 78-79 0.4845652388321078 1103 80-81 0.650385154396691 1103 82-83 0.5533694922588239 1103 84-85 0.4832118309061926 1103 86-87 0.438116295378844 1103 88-89 0.44893569840088077 1103 90-91 0.4625422915131665 1103 92-93 0.6352234064636555 1103 94-95 0.6915361959955781 1103 96-97 0.3946649591318696 1103 98-99 0.3609715069628514 1103 100-101 0.4321546858155578 1104 1 0.3883754265607564 1104 2 0.3743713268418851 1104 3 0.3781949129424689 1104 4 0.3963522694732262 1104 5 0.48405532831699105 1104 6 0.31814410550200733 1104 7 0.33185855846388534 1104 8 0.3459465945035447 1104 9 0.446748049438213 1104 10-11 0.4463559239991781 1104 12-13 0.45176402492897694 1104 14-15 0.5507483311180863 1104 16-17 0.4698124716336167 1104 18-19 0.5015956787201574 1104 20-21 0.698070443418473 1104 22-23 0.5198740340342383 1104 24-25 0.6340564755713913 1104 26-27 0.45515993935466525 1104 28-29 0.44806092397240604 1104 30-31 0.3131388956484358 1104 32-33 0.44431544151364477 1104 34-35 0.5314927984665516 1104 36-37 0.47856931512898626 1104 38-39 0.4943914406946419 1104 40-41 0.5954914730450085 1104 42-43 0.528378533134152 1104 44-45 0.32702402103841166 1104 46-47 0.39139735432063105 1104 48-49 0.5668664874864859 1104 50-51 0.3913286939575329 1104 52-53 0.31443276068994663 1104 54-55 0.3041603830680586 1104 56-57 0.5287111145377423 1104 58-59 0.562780075512066 1104 60-61 0.4762619522124609 1104 62-63 0.6093034600823586 1104 64-65 0.6689679775314303 1104 66-67 0.4659560296796954 1104 68-69 0.4428370402460118 1104 70-71 0.4414939047383939 1104 72-73 0.4425341900980051 1104 74-75 0.3852168875509676 1104 76-77 0.27575879995738717 1104 78-79 0.5330878237033794 1104 80-81 0.6428112589938486 1104 82-83 0.536679657970307 1104 84-85 0.4770124929634356 1104 86-87 0.5690150019354441 1104 88-89 0.5690577364505529 1104 90-91 0.570729279963313 1104 92-93 0.5375732496029713 1104 94-95 0.5533977445347595 1104 96-97 0.5650682400614677 1104 98-99 0.3893348650959503 1104 100-101 0.4972733390921036 1105 1 0.16266899860562134 1105 2 0.21669768577199733 1105 3 0.1805514703195179 1105 4 0.3033176850613728 1105 5 0.37166857249300733 1105 6 0.2797039990884329 1105 7 0.1616489668619323 1105 8 0.17101130546126342 1105 9 0.23079859588629859 1105 10-11 0.2816818155723766 1105 12-13 0.38114909107795825 1105 14-15 0.23591604035019031 1105 16-17 0.3403501118406993 1105 18-19 0.23853215412999162 1105 20-21 0.2697027773270193 1105 22-23 0.27052473866466187 1105 24-25 0.1387722866153993 1105 26-27 0.12243237248667782 1105 28-29 -0.04246161701119888 1105 30-31 -0.17829409255978845 1105 32-33 0.03719364111099566 1105 34-35 -0.2689179363623282 1105 36-37 -0.05672152467544578 1105 38-39 0.10372689510936794 1105 40-41 0.12495652912783584 1105 42-43 0.17844054780196217 1105 44-45 0.15025705238728193 1105 46-47 0.1835933925719999 1105 48-49 -0.053532743172127084 1105 50-51 0.06109160019855153 1105 52-53 0.25245134711503425 1105 54-55 0.27681371208732486 1105 56-57 0.29329265034447616 1105 58-59 0.28925135103694544 1105 60-61 0.15291118287107963 1105 62-63 0.1552113907697361 1105 64-65 0.1718422225702625 1105 66-67 0.15234425947549823 1105 68-69 0.2791012407061757 1105 70-71 0.2863709539187269 1105 72-73 0.197714661768984 1105 74-75 0.2930550023050671 1105 76-77 0.22129464263785792 1105 78-79 0.33130917112932323 1105 80-81 0.3799104105612905 1105 82-83 0.27910038704709805 1105 84-85 0.38730602905776834 1105 86-87 0.18853254579509482 1105 88-89 0.27010479569702994 1105 90-91 0.33619340133230935 1105 92-93 0.2137128814695224 1105 94-95 0.3913642026883366 1105 96-97 0.39323008935683745 1105 98-99 0.27752421654835047 1105 100-101 0.3565791343178759 1106 1 0.1596118645937139 1106 2 0.271516695337251 1106 3 0.3523234868923453 1106 4 0.3009149192586449 1106 5 0.3288621863993697 1106 6 0.2706162616689838 1106 7 0.27453242566807745 1106 8 0.44808283653820524 1106 9 0.355716016257837 1106 10-11 0.3281529891337769 1106 12-13 0.39140578231839385 1106 14-15 0.5533292268581533 1106 16-17 0.4664965823029874 1106 18-19 0.28638102111337815 1106 20-21 0.4610112654037266 1106 22-23 0.4561713383610595 1106 24-25 0.5706889392977743 1106 26-27 0.46476501673219417 1106 28-29 0.5420039613885734 1106 30-31 0.5459938058236276 1106 32-33 0.4834227900561956 1106 34-35 0.39328038880225336 1106 36-37 0.33652013442041806 1106 38-39 0.3225748453986057 1106 40-41 0.3557804544112386 1106 42-43 0.4575471543651304 1106 44-45 0.5644560950904705 1106 46-47 0.7029277549560007 1106 48-49 0.6003620496578819 1106 50-51 0.4444702980781443 1106 52-53 0.4452359008426612 1106 54-55 0.5099738572538755 1106 56-57 0.5912467611463228 1106 58-59 0.4875501142472771 1106 60-61 0.6516152251981424 1106 62-63 0.7782044646897077 1106 64-65 0.705218256201988 1106 66-67 0.6921554161400181 1106 68-69 0.52914253052041 1106 70-71 0.4601093300825525 1106 72-73 0.40293823918117866 1106 74-75 0.6170526182251663 1106 76-77 0.594087717136496 1106 78-79 0.6325368107359104 1106 80-81 0.6089496514363013 1106 82-83 0.7544628453112345 1106 84-85 0.7845857717083042 1106 86-87 0.8022518068846303 1106 88-89 0.6429944341115785 1106 90-91 0.7681009521724285 1106 92-93 0.5729992439924025 1106 94-95 0.5692224584329857 1106 96-97 0.5724307161422466 1106 98-99 0.5890490536072903 1106 100-101 0.3912628796572477 1107 1 0.2345731494032144 1107 2 0.31080473992003377 1107 3 0.2793944078919637 1107 4 0.22917944473461205 1107 5 0.22393991984632322 1107 6 0.22030053825054807 1107 7 0.10585399528827111 1107 8 0.31294774637311207 1107 9 0.30249299893316106 1107 10-11 0.3057065575274365 1107 12-13 0.39007199498037437 1107 14-15 0.34870099370891694 1107 16-17 0.43042573142451346 1107 18-19 0.3038578841073658 1107 20-21 0.446163767005217 1107 22-23 0.5513776668536607 1107 24-25 0.40120881032240874 1107 26-27 0.28472089983667814 1107 28-29 0.4090562943427756 1107 30-31 0.38872479853696973 1107 32-33 0.4846452749355805 1107 34-35 0.23605874299960306 1107 36-37 0.34596586838080157 1107 38-39 0.27987843785926714 1107 40-41 0.46827633925092726 1107 42-43 0.5547004142257634 1107 44-45 0.5131406699840113 1107 46-47 0.5238573711556498 1107 48-49 0.5265405305486865 1107 50-51 0.3415108887121008 1107 52-53 0.49383478532224245 1107 54-55 0.41708735010686127 1107 56-57 0.4842234749843115 1107 58-59 0.5265443631250406 1107 60-61 0.5031830269953801 1107 62-63 0.494366259125421 1107 64-65 0.5335335897944162 1107 66-67 0.36299827228115333 1107 68-69 0.5298281132568192 1107 70-71 0.4714181471626375 1107 72-73 0.6001664335855104 1107 74-75 0.582070843779924 1107 76-77 0.5274273494877804 1107 78-79 0.3942814485483197 1107 80-81 0.42547672948826687 1107 82-83 0.34322593211526353 1107 84-85 0.2180140657084273 1107 86-87 0.2902160474785944 1107 88-89 0.41288471234989643 1107 90-91 0.47308175205262515 1107 92-93 0.27880575004599706 1107 94-95 0.4344932389526228 1107 96-97 0.215227729694643 1107 98-99 0.2716830176743663 1107 100-101 0.2796275832884554 1108 1 -0.13329523441130675 1108 2 0.11733567650058063 1108 3 0.0031550475987423 1108 4 0.17893950696809213 1108 5 0.2589651067496135 1108 6 0.16795783410061205 1108 7 0.10005277495834974 1108 8 0.1603769164103639 1108 9 0.12826381839639822 1108 10-11 0.11123980161490721 1108 12-13 0.21021233053530608 1108 14-15 0.2764566986439263 1108 16-17 0.25877501686096593 1108 18-19 0.3535734427173267 1108 20-21 0.3831443691523688 1108 22-23 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2316 98-99 -2.4702506714276744 2316 100-101 -2.8283636295902674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6187.0 3 2577.0 4 2568.0 5 2875.0 6 3232.0 7 3806.0 8 4865.0 9 6057.0 10 7351.0 11 9398.0 12 11744.0 13 14040.0 14 15706.0 15 18788.0 16 23360.0 17 29681.0 18 26740.0 19 17796.0 20 17860.0 21 18952.0 22 20461.0 23 22868.0 24 25894.0 25 29501.0 26 34530.0 27 40401.0 28 47825.0 29 56961.0 30 68792.0 31 84745.0 32 106008.0 33 135936.0 34 181879.0 35 252144.0 36 366218.0 37 504630.0 38 417184.0 39 68417.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98991350315545 18.81732980421677 14.391208924340932 22.801547768286852 2 15.182920456312296 20.35050321175814 40.588660442211804 23.87791588971776 3 18.848430122299593 25.25572488777355 29.958691802437126 25.93715318748973 4 12.495664741485626 16.75140196443075 37.696416297100114 33.05651699698351 5 13.603821261159538 37.45252704466698 33.975284320466606 14.968367373706885 6 31.12404088764763 36.39709929698958 17.803009313133327 14.675850502229462 7 27.856183925026546 33.26956280873459 21.054762014680765 17.8194912515581 8 26.185695438909672 34.42310015527635 20.078491584302718 19.312712821511262 9 26.72694358144309 16.5310386834328 20.583699598453506 36.1583181366706 10-11 24.634531732644067 26.841395322552824 27.42549448422611 21.098578460577 12-13 25.826551873618552 24.279494339738342 28.05037319807785 21.843580588565256 14-15 22.7986053786733 25.212046337179995 26.71689667207747 25.272451612069236 16-17 21.815719683574237 28.757999146462243 27.07779289391959 22.348488276043934 18-19 21.771429500039886 26.869956743596486 29.969648128067316 21.388965628296305 20-21 22.61512574751223 25.96569051811757 30.060591332403618 21.35859240196658 22-23 22.24897010298047 26.022446307789178 29.955295729551167 21.773287859679186 24-25 22.017319714936125 26.23238315119401 29.97066605070359 21.77963108316628 26-27 21.820731497910824 26.479714771575384 29.83882969196881 21.860724038544983 28-29 21.62655754582994 26.46555728954713 30.04315817228261 21.86472699234032 30-31 21.820335700881017 26.232308938754194 30.002721559701122 21.944633800663663 32-33 21.61956515116238 26.459080387230642 29.86829571729822 22.053058744308757 34-35 22.092352118995347 26.44027614424641 29.676293064451503 21.791078672306735 36-37 22.042953376026496 26.265137521851873 29.59598316990435 22.095925932217284 38-39 21.83075901887092 26.527652762506683 29.730345522206868 21.91124269641553 40-41 22.011679813796537 26.357368919671693 29.69211103891776 21.938840227614012 42-43 21.88015831574967 26.468294383395012 29.392992482291397 22.258554818563915 44-45 21.996192769215714 26.67098103585481 29.173875232874387 22.158950962055094 46-47 22.17508420865467 26.812408615861376 28.751400246417518 22.26110692906643 48-49 22.223186443281676 27.009485411837996 28.41838470857003 22.348943436310297 50-51 22.177406474848706 27.170073576399425 28.275630571105665 22.376889377646208 52-53 22.419689452095064 27.187398910187195 28.082676141844054 22.31023549587369 54-55 22.170395638787465 27.1182669627072 28.162686890974303 22.548650507531033 56-57 22.168968429433267 27.481806663744457 28.024624842641998 22.324600064180277 58-59 22.348097733114496 27.07005422703715 28.116743689406793 22.46510435044156 60-61 22.38419842919331 26.980551037923 28.42452550086635 22.210725032017336 62-63 22.248381802927085 27.02752855294333 28.49575989530095 22.228329748828635 64-65 22.116755921585657 27.070090037792088 28.350881507724964 22.462272532897288 66-67 22.054366996523424 27.414119154312505 28.008315573733412 22.523198275430666 68-69 22.035422579817144 27.250604318592696 28.27767816087709 22.43629494071307 70-71 22.085254943519388 27.296249912041954 28.10779104233382 22.510704102104842 72-73 22.17957769172541 27.242043423799117 28.007096519348206 22.57128236512726 74-75 22.08437173647475 27.514730012105343 27.828231576750728 22.572666674669183 76-77 22.182346080215872 27.284479056714066 27.917307335344688 22.61586752772537 78-79 22.162102267533285 27.422147900751902 27.797573923136376 22.618175908578436 80-81 22.065970352557738 27.609556976721205 27.726190174918397 22.598282495802664 82-83 22.219141768866503 27.539690133071893 27.72119881804175 22.519969280019858 84-85 22.147024723883575 27.42846347300037 27.609518578831917 22.814993224284137 86-87 22.16234602736569 27.291270870696966 27.813056761204656 22.733326340732688 88-89 22.18946914344205 27.349768718698453 27.628019080130308 22.832743057729193 90-91 22.07814249875154 27.378426615684198 27.85701516176788 22.686415723796383 92-93 22.17423361471436 27.216041821505037 27.801848012872703 22.807876550907903 94-95 22.031122757061897 27.1195476545289 27.779710562421865 23.069619025987336 96-97 22.04131335821728 27.11013694542054 27.866313672159798 22.982236024202383 98-99 21.826459966379005 27.14187934044932 27.698830948306334 23.33282974486534 100-101 21.93224798947744 27.114916055753618 27.670191365138308 23.28264458963064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 468.0 1 378.5 2 398.0 3 547.5 4 479.5 5 451.5 6 856.0 7 1884.0 8 3260.0 9 3947.5 10 3532.0 11 3115.5 12 3290.5 13 3567.0 14 3852.5 15 4060.5 16 4280.5 17 4550.0 18 4745.5 19 5070.0 20 5623.5 21 6478.5 22 7734.5 23 9441.0 24 11331.0 25 13619.0 26 17086.5 27 21231.5 28 24536.0 29 28139.0 30 33040.5 31 37903.0 32 42880.5 33 48371.0 34 55054.0 35 59895.0 36 65220.5 37 72221.0 38 75673.5 39 76257.0 40 77722.0 41 81715.0 42 85025.5 43 88519.0 44 91872.0 45 94480.0 46 95402.0 47 97396.5 48 103852.5 49 104706.0 50 100264.0 51 96335.5 52 92600.5 53 91423.5 54 89296.0 55 82179.5 56 74347.0 57 67251.5 58 61241.5 59 55355.5 60 48067.0 61 39768.5 62 31836.0 63 24950.0 64 18758.5 65 14129.5 66 10364.5 67 8061.0 68 5693.0 69 3455.5 70 2321.5 71 1511.0 72 1081.5 73 845.0 74 605.5 75 454.0 76 332.0 77 189.0 78 101.0 79 64.0 80 43.0 81 30.0 82 23.5 83 18.5 84 14.5 85 13.0 86 10.5 87 8.0 88 5.0 89 6.0 90 6.5 91 3.5 92 4.0 93 3.5 94 2.0 95 1.5 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0230427849864439 2 0.011041334472671036 3 0.011484464953179573 4 0.020494784723519816 5 0.05409884616208384 6 0.03973403308559878 7 0.014069392756146035 8 0.04501467131165884 9 0.02669861145063933 10-11 0.021750321084960667 12-13 0.03439800354947515 14-15 0.01702359595953628 16-17 0.01667278432913369 18-19 0.015878842218222562 20-21 0.01322005933517134 22-23 0.0077917109489417675 24-25 0.0019017683121824692 26-27 3.6927540042378045E-5 28-29 0.0022156524025426826 30-31 0.0 32-33 2.769565503178353E-4 34-35 3.6927540042378045E-5 36-37 0.0 38-39 1.2924639014832317E-4 40-41 1.8463770021189023E-5 42-43 0.0 44-45 3.6927540042378045E-5 46-47 1.8463770021189023E-5 48-49 2.0310147023307925E-4 50-51 1.661739301907012E-4 52-53 6.646957207628048E-4 54-55 0.001606347991843445 56-57 3.323478603814024E-4 58-59 0.0014586378316739327 60-61 0.003618898924153048 62-63 0.0019940871622884143 64-65 0.0011816812813560975 66-67 0.011650638883370272 68-69 0.015177218957417377 70-71 0.027677191261762345 72-73 0.017780610530405028 74-75 0.019331567212184906 76-77 0.03225620622701722 78-79 0.02025475571324436 80-81 0.045402410482103805 82-83 0.035930496461233835 84-85 0.02005165424301128 86-87 0.014106320296188414 88-89 0.013755508665785822 90-91 0.024741451828393293 92-93 0.03257009031737744 94-95 0.012592291154450914 96-97 0.018611480181358532 98-99 0.028397278292588715 100-101 0.039161656214941915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2708006.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.357703738718705 #Duplication Level Percentage of deduplicated Percentage of total 1 73.91546147636238 37.961283722136955 2 13.940727524208144 14.319275081809668 3 5.071287761043776 7.813490832164292 4 2.3434602365869153 4.814189462163938 5 1.2544725659885771 3.2213415196195796 6 0.7694714651114174 2.3710972524354004 7 0.5121977675435617 1.841371084079475 8 0.36369210933991225 1.4942713282871123 9 0.26254268328670305 1.2135230412306075 >10 1.3925682466689366 12.994136027658323 >50 0.10906547983301237 3.8813937074554743 >100 0.06137905304044519 5.668724040340611 >500 0.0030253431651645086 1.0009458719550017 >1k 4.3219188072988107E-4 0.31219212083024067 >5k 1.4406396024329367E-4 0.5984884619968757 >10k+ 7.203198012164684E-5 0.4942764458363368 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13361 0.49338886250621306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9692 0.357901718090728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6486 0.239512024714864 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 3.6927540042378045E-5 12-13 0.0 0.0 0.0 0.0 9.231885010594512E-5 14-15 0.0 0.0 0.0 0.0 1.1078262012713413E-4 16-17 0.0 0.0 0.0 0.0 1.4771016016951218E-4 18-19 0.0 0.0 0.0 0.0 1.4771016016951218E-4 20-21 0.0 0.0 0.0 1.8463770021189023E-5 1.4771016016951218E-4 22-23 0.0 0.0 0.0 7.385508008475609E-5 1.4771016016951218E-4 24-25 0.0 0.0 0.0 2.0310147023307925E-4 1.4771016016951218E-4 26-27 0.0 0.0 0.0 5.539131006356706E-4 1.4771016016951218E-4 28-29 0.0 0.0 0.0 0.001624811761864634 1.4771016016951218E-4 30-31 0.0 0.0 0.0 0.0034527249939623472 1.8463770021189023E-4 32-33 0.0 0.0 0.0 0.006093044106992377 1.8463770021189023E-4 34-35 0.0 0.0 0.0 0.008548725519810518 1.8463770021189023E-4 36-37 0.0 0.0 0.0 0.010542812682098932 1.8463770021189023E-4 38-39 0.0 0.0 0.0 0.01301695786493826 1.8463770021189023E-4 40-41 0.0 0.0 0.0 0.017115914809642224 1.8463770021189023E-4 42-43 0.0 0.0 0.0 0.021953422555193745 1.8463770021189023E-4 44-45 0.0 0.0 0.0 0.027104814391105486 2.2156524025426829E-4 46-47 0.0 0.0 0.0 0.03345635127839451 2.769565503178353E-4 48-49 0.0 0.0 0.0 0.039290902605090244 2.9542032033902436E-4 50-51 0.0 0.0 0.0 0.04761806288464649 3.323478603814024E-4 52-53 0.0 0.0 0.0 0.06923913757945883 3.323478603814024E-4 54-55 0.0 0.0 0.0 0.1014399524964125 3.323478603814024E-4 56-57 0.0 0.0 0.0 0.12714152036590762 3.323478603814024E-4 58-59 0.0 0.0 0.0 0.1643644807286247 3.323478603814024E-4 60-61 0.0 0.0 0.0 0.20801283305871554 3.323478603814024E-4 62-63 0.0 0.0 0.0 0.23578234317058383 3.323478603814024E-4 64-65 0.0 0.0 0.0 0.2575142004855233 3.323478603814024E-4 66-67 0.0 0.0 0.0 0.3221004680196425 3.323478603814024E-4 68-69 0.0 0.0 0.0 0.3947184755129789 3.323478603814024E-4 70-71 0.0 0.0 0.0 0.4237619857563093 3.323478603814024E-4 72-73 0.0 0.0 0.0 0.4858925718776103 3.8773917044496945E-4 74-75 0.0 0.0 0.0 0.5784329872238096 4.431304805085365E-4 76-77 0.0 0.0 0.0 0.6616491987093086 4.431304805085365E-4 78-79 0.0 0.0 0.0 0.7456593523057187 4.431304805085365E-4 80-81 0.0 0.0 0.0 0.8633658861907987 4.431304805085365E-4 82-83 0.0 0.0 0.0 0.9962127114932537 4.800580205509146E-4 84-85 1.8463770021189023E-5 0.0 0.0 1.1235388695593733 4.800580205509146E-4 86-87 3.6927540042378045E-5 0.0 0.0 1.3388448917764584 4.985217905721037E-4 88-89 3.6927540042378045E-5 0.0 0.0 1.6044277597612413 5.169855605932927E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7825 0.0 43.535328 1 GTATCAA 13570 0.0 38.829224 1 ATCAACG 16615 0.0 31.48895 3 TCAACGC 16855 0.0 30.89961 4 TATCAAC 16980 0.0 30.78408 2 CAACGCA 17050 0.0 30.601954 5 AACGCAG 17510 0.0 29.876125 6 ACGCAGA 19535 0.0 26.389025 7 CGCAGAG 19540 0.0 26.163435 8 GTACATG 12545 0.0 25.148056 1 TACATGG 12685 0.0 24.274805 2 GCAGAGT 20575 0.0 23.944946 9 GAGTACT 10960 0.0 23.535751 12-13 ACATGGG 12930 0.0 22.638798 3 GTACTTT 11930 0.0 21.420052 14-15 AGTACTT 11495 0.0 20.683971 12-13 AGAGTAC 17725 0.0 20.584192 10-11 CAGAGTA 20015 0.0 20.187271 10-11 CATGGGG 5620 0.0 19.954779 4 ACTTTTT 14225 0.0 18.331532 16-17 >>END_MODULE