FastQCFastQC Report
Thu 26 May 2016
SRR936953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708006
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136400.5036916461780365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109070.4027686792422173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73970.2731530136934704No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.10380331505912468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88650.044.106031
GTATCAA147900.036.940821
ATCAACG186600.029.0411993
TATCAAC188900.028.8154412
TCAACGC188450.028.7807734
CAACGCA191700.028.2273735
AACGCAG197300.027.4743486
ACGCAGA223900.024.061787
CGCAGAG223050.024.0043838
GAGTACT127200.022.6825412-13
GTACATG129750.022.5553821
GCAGAGT237250.022.2072169
TACATGG130800.022.0058162
AGTACTT132800.021.22536312-13
ACATGGG132600.021.0249563
GTACTTT138750.020.67456814-15
GCTTATA11400.020.4205821
AGAGTAC204850.020.31594310-11
CATGGGG56300.019.7396744
CAGAGTA232700.019.12987110-11
Produced by FastQC (version 0.11.5)