##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936953_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2708006 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12389263539298 31.0 31.0 34.0 31.0 34.0 2 32.241514605211364 33.0 31.0 34.0 30.0 34.0 3 32.348042434174815 34.0 31.0 34.0 30.0 34.0 4 36.0215269094677 37.0 35.0 37.0 35.0 37.0 5 35.92248023084144 37.0 35.0 37.0 35.0 37.0 6 35.96267475035137 37.0 35.0 37.0 35.0 37.0 7 35.906625391524244 37.0 35.0 37.0 35.0 37.0 8 35.86562585164139 37.0 35.0 37.0 35.0 37.0 9 37.536953020045004 39.0 37.0 39.0 35.0 39.0 10-11 37.44384539768376 39.0 37.0 39.0 34.5 39.0 12-13 37.341935726878006 39.0 37.0 39.0 34.0 39.0 14-15 38.51997927626452 40.0 38.0 41.0 34.0 41.0 16-17 38.429990738572954 40.0 38.0 41.0 34.0 41.0 18-19 38.59259580665626 40.0 38.0 41.0 34.0 41.0 20-21 38.61973422510881 40.0 38.0 41.0 34.0 41.0 22-23 38.54312601227619 40.0 38.0 41.0 34.0 41.0 24-25 38.43617370123995 40.0 38.0 41.0 34.0 41.0 26-27 38.246269949180316 40.0 38.0 41.0 34.0 41.0 28-29 38.16435672594521 40.0 38.0 41.0 33.5 41.0 30-31 38.14226888714427 40.0 37.5 41.0 34.0 41.0 32-33 37.96866347415774 40.0 37.0 41.0 33.0 41.0 34-35 37.81185178319399 40.0 37.0 41.0 33.0 41.0 36-37 37.64381005802794 40.0 37.0 41.0 33.0 41.0 38-39 37.48602495710866 40.0 37.0 41.0 33.0 41.0 40-41 37.2923448101666 40.0 36.0 41.0 32.0 41.0 42-43 37.01763068471783 39.5 36.0 41.0 31.0 41.0 44-45 36.73011211939708 39.0 36.0 41.0 31.0 41.0 46-47 36.34326678005883 39.0 35.5 41.0 30.0 41.0 48-49 35.961555107337276 39.0 35.0 40.0 29.0 41.0 50-51 35.65108182921308 39.0 35.0 40.0 28.0 41.0 52-53 35.39486618567315 38.5 35.0 40.0 27.5 41.0 54-55 35.10106956926979 38.0 34.5 40.0 27.0 41.0 56-57 34.80833701993275 38.0 34.0 40.0 26.0 41.0 58-59 34.447076003524366 38.0 34.0 40.0 26.0 41.0 60-61 34.102168717499154 37.0 33.0 40.0 25.0 41.0 62-63 33.726820952390796 37.0 33.0 39.5 24.0 41.0 64-65 33.339308701679386 36.0 32.5 39.0 23.0 40.0 66-67 32.94163214557132 36.0 32.0 39.0 22.0 40.0 68-69 32.62911585129427 35.0 32.0 38.5 22.0 40.0 70-71 32.15862797202074 35.0 31.0 38.0 20.5 40.0 72-73 31.65054914944797 35.0 31.0 37.0 19.5 39.0 74-75 31.132276664084202 34.5 30.5 36.5 18.0 39.0 76-77 29.7364093728005 33.0 28.5 35.0 16.0 37.5 78-79 30.493066115806243 34.0 30.0 36.0 17.5 38.0 80-81 30.281890069667497 34.0 30.0 35.0 12.0 37.0 82-83 29.95560921947736 34.0 30.0 35.0 9.5 37.0 84-85 29.588615941028195 34.0 29.5 35.0 7.0 36.0 86-87 29.220419747962154 34.0 29.0 35.0 3.5 36.0 88-89 28.819691130669575 34.0 29.0 35.0 2.0 35.5 90-91 28.342975237130197 33.5 29.0 35.0 2.0 35.0 92-93 27.831755358001423 33.0 27.0 35.0 2.0 35.0 94-95 27.215325409175605 33.0 26.5 34.5 2.0 35.0 96-97 26.512533391728084 32.0 25.0 34.0 2.0 35.0 98-99 25.636700583381277 32.0 24.0 34.0 2.0 35.0 100-101 22.070401801177695 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24584784585834996 1101 2 0.21583703460292014 1101 3 0.22998360684037067 1101 4 0.22181320599835175 1101 5 0.2737320329452544 1101 6 0.2824408336655253 1101 7 0.2820232012378767 1101 8 0.2501878909551323 1101 9 0.37157029159117627 1101 10-11 0.3978817262790031 1101 12-13 0.452483923203026 1101 14-15 0.5642629819017415 1101 16-17 0.5584065265825799 1101 18-19 0.403026065200784 1101 20-21 0.4378294727904617 1101 22-23 0.42567276738810733 1101 24-25 0.3644118258468012 1101 26-27 0.4245489629574948 1101 28-29 0.4711272271096192 1101 30-31 0.4901208439219289 1101 32-33 0.5146568047945763 1101 34-35 0.5475617430697142 1101 36-37 0.5137318034114529 1101 38-39 0.5793709985705604 1101 40-41 0.5938155312045126 1101 42-43 0.6798815602365025 1101 44-45 0.6630368796786925 1101 46-47 0.695432092869936 1101 48-49 0.6999844249613787 1101 50-51 0.7029037741712401 1101 52-53 0.7751101539049543 1101 54-55 0.6901547103267376 1101 56-57 0.7722207625872173 1101 58-59 0.7960393350963528 1101 60-61 0.8731296876316534 1101 62-63 0.8385600701547418 1101 64-65 0.6975270994635778 1101 66-67 0.6088000456702005 1101 68-69 0.49052742304096597 1101 70-71 0.5808286728586012 1101 72-73 0.8317718740654776 1101 74-75 0.8070514274198253 1101 76-77 0.647924810275402 1101 78-79 0.716182980122742 1101 80-81 1.0008248306947074 1101 82-83 0.8772284702714366 1101 84-85 0.9041180965087889 1101 86-87 0.5256873459425755 1101 88-89 0.5572435686807324 1101 90-91 0.9199663066294406 1101 92-93 1.0094367107636337 1101 94-95 0.9355023368307762 1101 96-97 0.9700230392565103 1101 98-99 0.9589921355214592 1101 100-101 1.39192337651804 1102 1 0.2564624943677387 1102 2 0.21883666654992595 1102 3 0.21051483000317006 1102 4 0.15664942241351554 1102 5 0.23147218752751542 1102 6 0.18963749665166318 1102 7 0.20725814173597712 1102 8 0.26449779158082265 1102 9 0.2869573606624556 1102 10-11 0.275551950791332 1102 12-13 0.28872732892012465 1102 14-15 0.3211983272581378 1102 16-17 0.27966588126298575 1102 18-19 0.345733095013081 1102 20-21 0.3212020651104197 1102 22-23 0.2780847413790326 1102 24-25 0.2637266338458204 1102 26-27 0.33863496467507304 1102 28-29 0.37570273931670783 1102 30-31 0.3568083669252431 1102 32-33 0.361939346813962 1102 34-35 0.3405503334267266 1102 36-37 0.25446852283238286 1102 38-39 0.338880261237712 1102 40-41 0.3709526922890518 1102 42-43 0.4143405223270449 1102 44-45 0.2998145756669075 1102 46-47 0.27456471463413834 1102 48-49 0.12500282761136106 1102 50-51 0.2564505782443618 1102 52-53 0.26953905833386216 1102 54-55 0.22455294367234302 1102 56-57 0.21592030865518552 1102 58-59 0.2767742142522849 1102 60-61 0.26249050225563053 1102 62-63 0.34616833907880107 1102 64-65 0.2793323994267709 1102 66-67 0.12764374582969396 1102 68-69 0.15390921668591773 1102 70-71 0.056861674944236995 1102 72-73 0.18907527242150834 1102 74-75 0.21135642067218186 1102 76-77 0.11766471948899593 1102 78-79 0.1939918379316019 1102 80-81 0.4227810323877499 1102 82-83 0.39938587427094774 1102 84-85 0.3874005158438365 1102 86-87 0.3902763534263194 1102 88-89 0.41842195170121954 1102 90-91 0.5880272807430487 1102 92-93 0.3418744484645302 1102 94-95 0.37819403110141536 1102 96-97 0.5429018271352994 1102 98-99 0.4123953989247191 1102 100-101 0.8605959320327017 1103 1 0.12306256096780288 1103 2 0.20097119868445645 1103 3 0.16174693123527106 1103 4 0.09552721129130504 1103 5 0.03546819152352043 1103 6 0.07964748666165633 1103 7 -0.003347918870083788 1103 8 0.028267355350386936 1103 9 0.13394370764880392 1103 10-11 0.14947802471740346 1103 12-13 0.21155450174729395 1103 14-15 0.27782503388484514 1103 16-17 0.34332721992432624 1103 18-19 0.2682189424989261 1103 20-21 0.199906693815052 1103 22-23 0.18394554723982992 1103 24-25 0.12905297417216133 1103 26-27 0.12310882414893598 1103 28-29 0.10528149389546115 1103 30-31 0.1198286799455559 1103 32-33 0.15517944005405582 1103 34-35 0.25107314394953306 1103 36-37 0.1151828085466704 1103 38-39 0.12189724425470416 1103 40-41 0.17207656841291907 1103 42-43 0.28168151466804403 1103 44-45 0.2946114454637794 1103 46-47 0.2884175284869528 1103 48-49 0.22191424952278993 1103 50-51 0.3339897057834875 1103 52-53 0.3105979993928045 1103 54-55 0.38176240088179725 1103 56-57 0.4071041248389946 1103 58-59 0.3497845372626074 1103 60-61 0.21786845763358542 1103 62-63 0.42408209199255253 1103 64-65 0.22081591591028626 1103 66-67 0.003859197045137819 1103 68-69 0.046924534701233256 1103 70-71 0.0051383982209571855 1103 72-73 0.0550259883722255 1103 74-75 0.20862581794158075 1103 76-77 0.05927311109738653 1103 78-79 0.25028554422530647 1103 80-81 0.4409212255279442 1103 82-83 0.38792233780741014 1103 84-85 0.43054903399235656 1103 86-87 0.29631198446194773 1103 88-89 0.27169367997294813 1103 90-91 0.2934635111792794 1103 92-93 0.08056908715916578 1103 94-95 0.044577647485031235 1103 96-97 -0.10902124978778005 1103 98-99 0.09328117981049999 1103 100-101 0.22403249546926318 1104 1 0.25499258270046 1104 2 0.2701133503923998 1104 3 0.2556634314017714 1104 4 0.10108952178782715 1104 5 0.14037676978735902 1104 6 0.04825223717429594 1104 7 0.09790039422033203 1104 8 0.2063063120077615 1104 9 0.16931151966135616 1104 10-11 0.300052778910576 1104 12-13 0.19128874776443894 1104 14-15 0.16043622751578113 1104 16-17 0.3007530178106421 1104 18-19 0.22348659151658268 1104 20-21 0.22087771840450188 1104 22-23 0.18770127616003407 1104 24-25 0.13390743988188802 1104 26-27 0.019757296290826787 1104 28-29 0.09732240254821534 1104 30-31 0.1724563702377182 1104 32-33 0.17595066444370389 1104 34-35 0.20414069043813043 1104 36-37 0.1465059262184738 1104 38-39 0.15477670032494473 1104 40-41 0.07478044348521706 1104 42-43 0.09879603392072056 1104 44-45 0.0039908270471116225 1104 46-47 0.12637507532608083 1104 48-49 0.0835370565403295 1104 50-51 0.18735552855721238 1104 52-53 0.04493471530846449 1104 54-55 0.09893760506358262 1104 56-57 0.0706198853942368 1104 58-59 0.16450424737705305 1104 60-61 0.196770038508852 1104 62-63 0.2635381403301835 1104 64-65 0.2438428889372588 1104 66-67 0.2053351333895037 1104 68-69 0.1696940897076331 1104 70-71 0.11557647987614672 1104 72-73 0.19899775125846375 1104 74-75 0.16722686601631054 1104 76-77 0.18860463483680334 1104 78-79 0.26063473450870944 1104 80-81 0.49214062016838866 1104 82-83 0.520989435183715 1104 84-85 0.4082518684386116 1104 86-87 0.2856434862934485 1104 88-89 0.15426111254037878 1104 90-91 0.17635885048909117 1104 92-93 0.18449153532503715 1104 94-95 0.17887952981322996 1104 96-97 0.42585637258984477 1104 98-99 0.32015441622321106 1104 100-101 0.2451032010794414 1105 1 0.171254805881361 1105 2 0.17537111570732122 1105 3 0.13390796692089424 1105 4 0.1232907728663406 1105 5 0.08809930358086149 1105 6 0.11206622912138187 1105 7 0.07291836948636643 1105 8 0.04343422400086183 1105 9 0.15328297493888954 1105 10-11 0.1549744067506751 1105 12-13 0.13565260309949423 1105 14-15 0.17263514180970674 1105 16-17 0.10138934222824503 1105 18-19 0.12966828550565168 1105 20-21 0.16159169597136724 1105 22-23 0.0741038861686576 1105 24-25 0.03770920864806726 1105 26-27 0.09809080937682069 1105 28-29 0.12053655986774459 1105 30-31 0.01285488188569417 1105 32-33 -0.016668691322763607 1105 34-35 0.1629270004755199 1105 36-37 0.10606607145862057 1105 38-39 0.11992176072183725 1105 40-41 0.06723622433077026 1105 42-43 0.153689800098455 1105 44-45 0.18719563722829946 1105 46-47 0.18999852945319873 1105 48-49 0.22276807465939896 1105 50-51 0.18832799044964332 1105 52-53 0.20213201182845353 1105 54-55 0.2605289553655652 1105 56-57 0.2371966757307291 1105 58-59 0.11644018352890839 1105 60-61 0.16290463730091176 1105 62-63 0.2219703376267006 1105 64-65 0.05706010205201295 1105 66-67 0.04533189784161351 1105 68-69 0.06970127981404772 1105 70-71 -0.232168522999892 1105 72-73 -0.15814472070012542 1105 74-75 0.013450262622583864 1105 76-77 -0.06848301256037814 1105 78-79 -0.025224123702393797 1105 80-81 0.06909632911288455 1105 82-83 0.1967596753332721 1105 84-85 0.2622001356229653 1105 86-87 0.16101916935356186 1105 88-89 0.03588679560049002 1105 90-91 0.14988577743761056 1105 92-93 0.12993570414873545 1105 94-95 0.05386514960040145 1105 96-97 0.08115008843683924 1105 98-99 -0.08864447650040219 1105 100-101 0.34053928103342557 1106 1 0.10542835378188187 1106 2 0.08612146435007872 1106 3 0.14055797781900026 1106 4 0.11522171845492579 1106 5 0.06482535042533044 1106 6 0.13916862207407377 1106 7 0.12110111133256396 1106 8 0.17847072299110067 1106 9 0.13517356380276624 1106 10-11 0.0792150719976874 1106 12-13 0.11515055271362229 1106 14-15 0.1970304901534874 1106 16-17 0.15741359242932873 1106 18-19 -0.025756701812049698 1106 20-21 0.14218614633099946 1106 22-23 0.10898479790562732 1106 24-25 0.15386872789719774 1106 26-27 0.1604874944255883 1106 28-29 0.15129096154277022 1106 30-31 0.17884843416474183 1106 32-33 0.09058771926748932 1106 34-35 0.1130539681612035 1106 36-37 0.22288749355457327 1106 38-39 0.28070031904788806 1106 40-41 0.42989358373081643 1106 42-43 0.09545181308359219 1106 44-45 -0.05246370552948321 1106 46-47 -0.09408178182739846 1106 48-49 0.09195778405625532 1106 50-51 0.09849341010364299 1106 52-53 -0.018593811267415106 1106 54-55 -0.09965969508318295 1106 56-57 -0.14522265180828242 1106 58-59 -0.01562073636716832 1106 60-61 -0.06155872772371396 1106 62-63 0.08738061250956974 1106 64-65 -0.1227825964139555 1106 66-67 -0.012032312316186733 1106 68-69 -0.097810833472181 1106 70-71 -0.1057306127539448 1106 72-73 -0.01438383465567128 1106 74-75 0.1538989822405128 1106 76-77 0.007799346598559964 1106 78-79 0.03557702648113903 1106 80-81 0.18070444501465843 1106 82-83 0.15531555172374567 1106 84-85 0.13873894973610135 1106 86-87 -0.14608524649863952 1106 88-89 -0.2203770801881788 1106 90-91 -0.1188661875958843 1106 92-93 0.13262333953110428 1106 94-95 0.0584755039526037 1106 96-97 0.14907822221921307 1106 98-99 -0.11008540301248715 1106 100-101 -0.13844583100482666 1107 1 0.11463395253919373 1107 2 0.1286139263271835 1107 3 0.147098616586959 1107 4 0.15837177413586545 1107 5 0.06418392023925179 1107 6 0.08378409079826099 1107 7 0.06790305238088479 1107 8 0.09669152377455248 1107 9 0.19718787089296796 1107 10-11 0.14186896710834418 1107 12-13 0.15058954078233455 1107 14-15 0.14743505349486696 1107 16-17 0.16534964194674018 1107 18-19 0.21959349387348226 1107 20-21 0.12178481569316801 1107 22-23 0.11061517390945852 1107 24-25 0.04117418629336811 1107 26-27 0.1275562285963403 1107 28-29 0.14487523348920206 1107 30-31 0.09238205249892673 1107 32-33 0.11002274489736408 1107 34-35 0.08876323163961786 1107 36-37 0.15517429853815656 1107 38-39 0.25625917861663083 1107 40-41 0.28703013336648553 1107 42-43 0.24575077873731033 1107 44-45 0.1642862151385529 1107 46-47 0.08308582315524404 1107 48-49 0.09211626972480502 1107 50-51 0.10752542931921738 1107 52-53 0.11022799902280411 1107 54-55 0.2669383360577342 1107 56-57 0.2229845407194162 1107 58-59 0.17291511039317697 1107 60-61 0.2372250269901528 1107 62-63 0.28624215415261745 1107 64-65 0.21808531317968516 1107 66-67 0.2347986279845813 1107 68-69 0.22950491728161637 1107 70-71 0.2383884814710413 1107 72-73 0.16953370287993863 1107 74-75 0.09383505315081564 1107 76-77 0.13310113492540765 1107 78-79 0.34005503399479764 1107 80-81 0.48551737012408935 1107 82-83 0.3975793474644185 1107 84-85 0.4085802620235839 1107 86-87 0.4671670553170202 1107 88-89 0.4045978128770784 1107 90-91 0.5158762835920498 1107 92-93 0.5048115114015914 1107 94-95 0.48354238644977343 1107 96-97 0.41066276989623773 1107 98-99 0.3048843914137116 1107 100-101 0.4110621324989019 1108 1 0.06129713527608516 1108 2 0.03702021935431077 1108 3 -0.03900790098660423 1108 4 0.013319789608331689 1108 5 0.05552396848928964 1108 6 0.12573251432003474 1108 7 0.06023673649345085 1108 8 0.06804980299959595 1108 9 0.0863755186915327 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66-67 -1.1736239740377172 2316 68-69 -1.4026830080017305 2316 70-71 -1.096385950200208 2316 72-73 -0.96634525739778 2316 74-75 -0.9503551028702155 2316 76-77 -0.9665464921077884 2316 78-79 -1.1399095981032694 2316 80-81 -1.0984446968868546 2316 82-83 -1.2276469261326852 2316 84-85 -1.1413070256449274 2316 86-87 -1.1070155517205116 2316 88-89 -1.3717668160864633 2316 90-91 -1.3173103800445745 2316 92-93 -1.2264812454924048 2316 94-95 -1.2741067712769656 2316 96-97 -1.0240757453310287 2316 98-99 -1.3044472231545186 2316 100-101 -1.3518995244115537 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 18.0 5 69.0 6 147.0 7 322.0 8 793.0 9 1610.0 10 2518.0 11 4402.0 12 6577.0 13 9456.0 14 11970.0 15 16483.0 16 21717.0 17 29462.0 18 26589.0 19 16617.0 20 14454.0 21 15136.0 22 15795.0 23 17625.0 24 20616.0 25 24397.0 26 29851.0 27 37296.0 28 46417.0 29 57454.0 30 71674.0 31 89504.0 32 113411.0 33 146675.0 34 197444.0 35 276876.0 36 413431.0 37 581389.0 38 368564.0 39 21234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95949579267011 19.6779520843468 14.941164607234 22.421387515749093 2 15.676305675607704 21.08365637548004 39.601846630409135 23.638191318503125 3 19.20983839382748 25.41496445904971 30.243134885744215 25.13206226137859 4 13.048463495343606 17.357921740681398 37.044317257367716 32.54929750660728 5 13.546471866363566 37.884840642776005 33.876057790165746 14.692629700694681 6 31.283202474440603 36.8463733093649 17.337553904976577 14.532870311217922 7 27.911090300390768 33.456757481334975 20.779643767406718 17.85250845086754 8 26.489601573999465 34.8948266732053 19.664579768287073 18.950991984508157 9 26.69433771355666 16.51134322130129 20.481202951988642 36.31311611315341 10-11 24.85451400827803 27.10532273570171 27.091325352732206 20.948837903288055 12-13 25.948299228288267 24.513775080262008 27.927984649960152 21.60994104148957 14-15 22.85781493837163 25.429190334142536 26.636573183368135 25.0764215441177 16-17 21.84025810873388 28.91474391120256 27.099053694858878 22.145944285204685 18-19 21.808178780208458 26.944099427048528 30.08354334414514 21.164178448597866 20-21 22.646722182760715 26.060775481091376 30.258973143669447 21.03352919247846 22-23 22.280197618160898 26.097717110308 30.135569367154 21.486515904377093 24-25 22.14785004116035 26.333944306299472 30.140473308926424 21.377732343613758 26-27 21.953907255029005 26.534856869790296 30.010582131726217 21.500653743454485 28-29 21.696739044337054 26.484858074859456 30.261582879876602 21.556820000926884 30-31 21.93045931285776 26.319390987676726 30.16973694438787 21.580412755077642 32-33 21.758959892136744 26.51040133713804 30.031762937183537 21.698875833541674 34-35 22.17181202700437 26.45376339638834 29.828035831530652 21.546388745076637 36-37 22.21202113545359 26.277041368874233 29.704600368014667 21.806337127657514 38-39 22.009276304576126 26.528487879625573 29.849428554597964 21.61280726120034 40-41 22.176648701705513 26.40186428400899 29.837536164262605 21.583950850022887 42-43 22.06915346568656 26.53182821603793 29.49116434749406 21.907853970781453 44-45 22.190478898495794 26.798611229074087 29.18992793959836 21.82098193283176 46-47 22.343377780699168 27.008412490855786 28.698259266904653 21.949950461540393 48-49 22.493815794599996 27.17728621723091 28.309027656604833 22.019870331564253 50-51 22.494371823071386 27.34075287576206 28.123379683315335 22.04149561785122 52-53 22.726445214670868 27.316760007178715 27.989764424451053 21.967030353699364 54-55 22.456009998831206 27.26433772976382 28.054668598533226 22.224983672871744 56-57 22.419891765415784 27.630931478818848 27.931347167718922 22.017829588046446 58-59 22.690291447132278 27.271322346643505 27.916613121694063 22.121773084530155 60-61 22.65074985565673 27.19201892055424 28.312163295210464 21.845067928578562 62-63 22.513783513848328 27.251113381461227 28.316920277658937 21.91818282703151 64-65 22.38959632836787 27.24102665227231 28.17381648597095 22.19556053338887 66-67 22.334550372072634 27.579941793371272 27.874907817347438 22.21060001720866 68-69 22.349571205766026 27.403115324387866 28.11448890057443 22.132824569271683 70-71 22.436010705426295 27.386301005641673 28.0098856247617 22.16780266417033 72-73 22.51656754084001 27.368477027008066 27.855329714926775 22.259625717225145 74-75 22.46839556485473 27.569215134678434 27.685887697442325 22.276501603024514 76-77 22.513188670926134 27.506715273156708 27.739303384113622 22.240792671803533 78-79 22.479885167831632 27.51158440031433 27.647018854100814 22.361511577753223 80-81 22.370157416562574 27.812868995556588 27.515816897884648 22.301156689996187 82-83 22.534821400212877 27.714266931691185 27.530529893923717 22.220381774172225 84-85 22.445308377031605 27.642974503401874 27.420651583422984 22.49106553614354 86-87 22.385383982331554 27.53208831193866 27.572304296518475 22.510223409211306 88-89 22.50009601167502 27.591447427699517 27.429741610425985 22.478714950199482 90-91 22.372571538299624 27.69378764323354 27.543676306042492 22.389964512424342 92-93 22.38326545428495 27.447569297375225 27.549415565640068 22.619749682699762 94-95 22.322447705968045 27.388391084949994 27.52472761313218 22.764433595949786 96-97 22.406508988819024 27.363676733023812 27.5701061412749 22.65970813688227 98-99 22.145181773565827 27.400730513815397 27.340574462593022 23.113513250025758 100-101 22.017035829229595 27.658637920220414 27.35959787274624 22.964728377803752 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 781.0 1 575.5 2 431.5 3 549.0 4 514.0 5 486.5 6 822.5 7 1833.5 8 3070.0 9 3659.5 10 3433.5 11 3120.5 12 3318.5 13 3659.0 14 4047.5 15 4395.0 16 4662.0 17 4993.5 18 5270.0 19 5560.0 20 6092.0 21 6875.5 22 8025.0 23 9576.0 24 11468.0 25 13949.5 26 17336.0 27 21365.5 28 24780.5 29 28414.0 30 33375.0 31 38107.5 32 42900.5 33 48395.0 34 55119.0 35 59875.0 36 65194.5 37 72309.5 38 76200.0 39 76779.5 40 77671.5 41 81560.5 42 84968.0 43 88113.5 44 91936.0 45 94709.5 46 95298.0 47 97153.0 48 103753.5 49 104887.5 50 100127.0 51 95961.5 52 92017.5 53 91106.5 54 88810.0 55 81820.0 56 73855.5 57 66475.5 58 60736.0 59 55324.0 60 48388.0 61 39485.5 62 31425.0 63 24825.0 64 18511.5 65 13908.0 66 10100.0 67 7843.5 68 5608.0 69 3265.0 70 2151.0 71 1473.0 72 1090.5 73 867.5 74 619.0 75 486.5 76 342.0 77 164.0 78 80.0 79 48.5 80 31.0 81 28.0 82 24.5 83 10.5 84 2.5 85 4.5 86 4.0 87 1.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026107770809961277 2 0.005723768706568597 3 1.1078262012713413E-4 4 3.323478603814024E-4 5 7.385508008475609E-5 6 0.0 7 0.0 8 0.0 9 3.6927540042378045E-5 10-11 0.013183131795128962 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.8773917044496945E-4 20-21 8.30869650953506E-4 22-23 0.0020864060123943597 24-25 0.011189044632840548 26-27 0.005151391835911737 28-29 4.2466671048734753E-4 30-31 0.0029726669734114327 32-33 0.0049113628256362805 34-35 0.0 36-37 0.008622580599895274 38-39 0.013090812945023015 40-41 0.0018279132320977132 42-43 0.0 44-45 0.0 46-47 3.323478603814024E-4 48-49 0.003674290234216616 50-51 0.007459363088560366 52-53 0.0 54-55 0.003305014833792835 56-57 0.0033788699138775913 58-59 7.385508008475609E-5 60-61 0.0019017683121824692 62-63 0.005594522316420274 64-65 1.8463770021189023E-5 66-67 0.002474145182839329 68-69 2.0310147023307925E-4 70-71 0.0012370725914196644 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0019940871622884143 80-81 0.002344898792691006 82-83 0.01225994329406951 84-85 0.008604116829874085 86-87 0.004708261355403201 88-89 7.385508008475609E-5 90-91 5.908406406780487E-4 92-93 1.1078262012713413E-4 94-95 3.6927540042378045E-5 96-97 0.0021602610924791156 98-99 0.001809449462076524 100-101 2.769565503178353E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2708006.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.719978308433454 #Duplication Level Percentage of deduplicated Percentage of total 1 71.64538705808896 34.90561703369413 2 15.058061498050318 14.67256859104137 3 5.518536893147543 8.06588993185314 4 2.4798664584070225 4.832761602456072 5 1.4027458388927132 3.4170873421549133 6 0.8768576784853852 2.5632292245234773 7 0.5837554742532532 1.9908387830133445 8 0.3909619205652725 1.523812503149085 9 0.30462873779402677 1.3357354947705404 >10 1.546378815292444 13.688713575137909 >50 0.11922553829685135 4.024563159228631 >100 0.06918974394763988 6.188686283458777 >500 0.0034171640524528766 1.0853657240562733 >1k 7.59369789426788E-4 0.5233152049173373 >5k 7.59369789426788E-5 0.2736629601112438 >10k+ 1.518739578853576E-4 0.908152586433784 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13640 0.5036916461780365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10907 0.4027686792422173 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7397 0.2731530136934704 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2811 0.10380331505912468 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.5391310063567065E-5 14-15 3.6927540042378045E-5 0.0 0.0 0.0 7.385508008475609E-5 16-17 3.6927540042378045E-5 0.0 0.0 0.0 1.1078262012713413E-4 18-19 3.6927540042378045E-5 0.0 0.0 0.0 1.4771016016951218E-4 20-21 3.6927540042378045E-5 0.0 0.0 1.8463770021189023E-5 1.4771016016951218E-4 22-23 3.6927540042378045E-5 0.0 0.0 7.385508008475609E-5 1.8463770021189023E-4 24-25 3.6927540042378045E-5 0.0 0.0 2.0310147023307925E-4 1.8463770021189023E-4 26-27 3.6927540042378045E-5 0.0 0.0 5.723768706568596E-4 2.0310147023307925E-4 28-29 3.6927540042378045E-5 0.0 0.0 0.001643275531885823 2.2156524025426826E-4 30-31 3.6927540042378045E-5 0.0 0.0 0.0034527249939623472 2.2156524025426826E-4 32-33 3.6927540042378045E-5 0.0 0.0 0.006148435417055944 2.2156524025426826E-4 34-35 3.6927540042378045E-5 0.0 0.0 0.008659508139937652 2.2156524025426826E-4 36-37 3.6927540042378045E-5 0.0 0.0 0.01074591415233201 2.2156524025426826E-4 38-39 3.6927540042378045E-5 0.0 0.0 0.013367769495340853 2.2156524025426826E-4 40-41 3.6927540042378045E-5 0.0 0.0 0.017706755450320272 2.2156524025426826E-4 42-43 3.6927540042378045E-5 0.0 0.0 0.022747364666104875 2.2156524025426826E-4 44-45 3.6927540042378045E-5 0.0 0.0 0.028194176822355636 2.5849278029664634E-4 46-47 3.6927540042378045E-5 0.0 0.0 0.034490322399581094 2.9542032033902436E-4 48-49 3.6927540042378045E-5 0.0 0.0 0.0401956273361285 2.9542032033902436E-4 50-51 3.6927540042378045E-5 0.0 0.0 0.04854125138570594 2.9542032033902436E-4 52-53 3.6927540042378045E-5 0.0 0.0 0.07027310870064542 2.9542032033902436E-4 54-55 3.6927540042378045E-5 0.0 0.0 0.10295398163814999 2.9542032033902436E-4 56-57 7.385508008475609E-5 0.0 0.0 0.12895096982798415 2.9542032033902436E-4 58-59 7.385508008475609E-5 0.0 0.0 0.16654320559112498 2.9542032033902436E-4 60-61 7.385508008475609E-5 0.0 0.0 0.2102469492312794 2.9542032033902436E-4 62-63 7.385508008475609E-5 0.0 0.0 0.23775796656285103 3.6927540042378047E-4 64-65 7.385508008475609E-5 0.0 0.0 0.2593605774876422 3.6927540042378047E-4 66-67 7.385508008475609E-5 0.0 0.0 0.3240945551819309 3.6927540042378047E-4 68-69 7.385508008475609E-5 0.0 0.0 0.39684180906541566 3.6927540042378047E-4 70-71 7.385508008475609E-5 0.0 0.0 0.4259591743888308 3.6927540042378047E-4 72-73 7.385508008475609E-5 0.0 0.0 0.4879605141199835 3.8773917044496945E-4 74-75 7.385508008475609E-5 0.0 0.0 0.5802609004559074 4.062029404661585E-4 76-77 7.385508008475609E-5 0.0 3.6927540042378045E-5 0.6632924742411945 4.062029404661585E-4 78-79 7.385508008475609E-5 0.0 3.6927540042378045E-5 0.7469702799772231 4.062029404661585E-4 80-81 7.385508008475609E-5 0.0 3.6927540042378045E-5 0.8644367848520276 4.062029404661585E-4 82-83 7.385508008475609E-5 0.0 3.6927540042378045E-5 0.9981514073454786 5.169855605932927E-4 84-85 7.385508008475609E-5 0.0 3.6927540042378045E-5 1.1254221741015344 5.169855605932927E-4 86-87 7.385508008475609E-5 0.0 3.6927540042378045E-5 1.3413190369592978 5.169855605932927E-4 88-89 7.385508008475609E-5 0.0 3.6927540042378045E-5 1.6070311513342288 5.169855605932927E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8865 0.0 44.10603 1 GTATCAA 14790 0.0 36.94082 1 ATCAACG 18660 0.0 29.041199 3 TATCAAC 18890 0.0 28.815441 2 TCAACGC 18845 0.0 28.780773 4 CAACGCA 19170 0.0 28.227373 5 AACGCAG 19730 0.0 27.474348 6 ACGCAGA 22390 0.0 24.06178 7 CGCAGAG 22305 0.0 24.004383 8 GAGTACT 12720 0.0 22.68254 12-13 GTACATG 12975 0.0 22.555382 1 GCAGAGT 23725 0.0 22.207216 9 TACATGG 13080 0.0 22.005816 2 AGTACTT 13280 0.0 21.225363 12-13 ACATGGG 13260 0.0 21.024956 3 GTACTTT 13875 0.0 20.674568 14-15 GCTTATA 1140 0.0 20.420582 1 AGAGTAC 20485 0.0 20.315943 10-11 CATGGGG 5630 0.0 19.739674 4 CAGAGTA 23270 0.0 19.129871 10-11 >>END_MODULE