FastQCFastQC Report
Thu 26 May 2016
SRR936952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686099
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132900.49476955242528287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97930.3645807544695858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64240.23915723136042266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74800.042.63811
GTATCAA133100.040.317431
ATCAACG167450.031.8505543
TCAACGC170200.031.33714
TATCAAC171450.031.2460942
CAACGCA173400.030.8404625
AACGCAG178550.030.0817836
ACGCAGA199350.026.7483437
CGCAGAG200200.026.4206358
GTACATG125250.025.425761
TACATGG128150.024.8151452
GCAGAGT209550.024.2652079
ACATGGG128900.023.7119563
GAGTACT112650.022.76000812-13
AGTACTT117800.021.72464212-13
AGAGTAC180350.021.56683310-11
GTACTTT120500.021.23746714-15
CATGGGG56000.020.7971364
CAGAGTA204000.019.50915510-11
TACTTTT135100.018.76649514-15
Produced by FastQC (version 0.11.5)