##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936952_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2686099 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50242191371204 31.0 31.0 34.0 30.0 34.0 2 31.576929964234377 33.0 31.0 34.0 30.0 34.0 3 31.589938047704123 33.0 31.0 34.0 28.0 34.0 4 35.20928379780492 37.0 35.0 37.0 33.0 37.0 5 35.207943564254336 37.0 35.0 37.0 33.0 37.0 6 35.267773079100955 37.0 35.0 37.0 32.0 37.0 7 35.19825032509971 37.0 35.0 37.0 32.0 37.0 8 35.155502831429516 37.0 35.0 37.0 32.0 37.0 9 36.73746351121087 39.0 37.0 39.0 32.0 39.0 10-11 36.595076912652885 39.0 37.0 39.0 32.0 39.0 12-13 36.48667156348296 39.0 37.0 39.0 32.0 39.0 14-15 37.55899633632268 40.0 37.0 41.0 32.0 41.0 16-17 37.488436762755214 40.0 37.0 41.0 32.0 41.0 18-19 37.45238057122988 40.0 37.0 41.0 32.0 41.0 20-21 37.3855375397556 40.0 37.0 41.0 32.0 41.0 22-23 37.26574951258311 40.0 37.0 41.0 31.0 41.0 24-25 37.2111664536564 39.5 36.5 41.0 31.0 41.0 26-27 37.22861834206408 40.0 37.0 41.0 31.0 41.0 28-29 37.15016311759172 40.0 36.5 41.0 31.0 41.0 30-31 36.96646195840138 39.0 36.0 41.0 30.5 41.0 32-33 36.76253965322946 39.0 36.0 41.0 30.0 41.0 34-35 36.57774974042282 39.0 35.0 41.0 30.0 41.0 36-37 36.385279172510025 39.0 35.0 41.0 30.0 41.0 38-39 36.217310121481006 39.0 35.0 41.0 30.0 41.0 40-41 36.01489167003896 39.0 35.0 40.0 29.0 41.0 42-43 35.77332406586652 39.0 35.0 40.0 28.0 41.0 44-45 35.49065782757821 38.0 35.0 40.0 27.0 41.0 46-47 35.11458773485266 38.0 34.5 40.0 25.5 41.0 48-49 34.833599022225165 38.0 34.0 40.0 24.0 41.0 50-51 34.1261018302006 37.5 33.5 39.5 23.5 40.5 52-53 34.304571611098474 38.0 33.5 39.5 24.0 40.5 54-55 34.68562178832575 38.0 34.0 40.0 24.0 41.0 56-57 34.55711852020346 38.0 34.0 40.0 23.5 41.0 58-59 34.32695797883846 38.0 34.0 40.0 23.0 41.0 60-61 34.034227145015876 38.0 34.0 40.0 22.5 41.0 62-63 33.71226767889047 37.0 33.0 40.0 22.0 41.0 64-65 33.37501484494801 37.0 33.0 40.0 21.0 41.0 66-67 33.00849912828976 36.0 32.5 39.0 20.0 41.0 68-69 32.60756156046371 36.0 32.0 39.0 18.5 41.0 70-71 32.19973705362312 35.0 31.5 39.0 18.0 40.0 72-73 31.812807160123285 35.0 31.0 38.0 14.5 40.0 74-75 31.419377320046657 35.0 31.0 37.0 12.0 39.5 76-77 30.966941836469914 35.0 31.0 37.0 8.0 39.0 78-79 30.506170100208518 35.0 30.0 36.0 7.0 39.0 80-81 30.073199461375026 34.0 30.0 36.0 3.5 37.5 82-83 29.67392638916138 34.0 29.5 35.0 2.0 37.0 84-85 29.300342243528625 34.0 29.0 35.0 2.0 36.5 86-87 28.946214566179428 34.0 29.0 35.0 2.0 36.0 88-89 28.57211833964422 34.0 29.0 35.0 2.0 36.0 90-91 28.194345405735234 34.0 28.5 35.0 2.0 35.5 92-93 27.79270272614673 34.0 27.0 35.0 2.0 35.0 94-95 27.397421874621898 34.0 26.5 35.0 2.0 35.0 96-97 26.87319231346276 33.0 25.5 35.0 2.0 35.0 98-99 26.26660223617968 33.0 25.0 35.0 2.0 35.0 100-101 24.058341111031275 31.0 12.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3685511392238574 1101 2 0.38934343151164796 1101 3 0.25452345959504186 1101 4 0.31008980390043206 1101 5 0.3489151213778783 1101 6 0.3122635392326458 1101 7 0.3284962695896496 1101 8 0.36153142171168184 1101 9 0.3262389450004619 1101 10-11 0.385060871108017 1101 12-13 0.5476797852034991 1101 14-15 0.5672358854493851 1101 16-17 0.5863820565263893 1101 18-19 0.5937455682593793 1101 20-21 0.5448710060847333 1101 22-23 0.6900790527464267 1101 24-25 0.82179986508784 1101 26-27 0.6258848569498028 1101 28-29 0.6765718592667582 1101 30-31 0.5606129809128007 1101 32-33 0.4341973988780907 1101 34-35 0.6176794966929364 1101 36-37 0.5580466623509395 1101 38-39 0.6829253714546439 1101 40-41 0.7382535115270912 1101 42-43 0.738662579754525 1101 44-45 0.8195544084466277 1101 46-47 0.7071058107058548 1101 48-49 0.7296098043182724 1101 50-51 0.8173625361820385 1101 52-53 0.857754051905232 1101 54-55 1.0223910647601002 1101 56-57 0.9017206721550153 1101 58-59 0.9717815229919111 1101 60-61 0.9331621689321352 1101 62-63 0.9916720918581348 1101 64-65 0.9807784068265519 1101 66-67 0.9715940671575325 1101 68-69 0.9674148173837835 1101 70-71 0.9180025904986309 1101 72-73 0.7157160065973613 1101 74-75 0.7542211376658798 1101 76-77 0.8078313198139959 1101 78-79 0.9903425498541374 1101 80-81 1.0098404002308143 1101 82-83 0.960891419499049 1101 84-85 0.9429733441405581 1101 86-87 0.9848361481816639 1101 88-89 1.0139348236986443 1101 90-91 1.1239164532401347 1101 92-93 1.2235861069966596 1101 94-95 1.163544527510716 1101 96-97 1.1373023290779933 1101 98-99 1.195144382018185 1101 100-101 1.4989186937619685 1102 1 0.2943631811655756 1102 2 0.2813182267882439 1102 3 0.32707433920870344 1102 4 0.3174962312466576 1102 5 0.35799406688104796 1102 6 0.2773034443487248 1102 7 0.3111236044760304 1102 8 0.21817884855230574 1102 9 0.34207659804095414 1102 10-11 0.35874452205993634 1102 12-13 0.2388893189586483 1102 14-15 0.24740695294077852 1102 16-17 0.4681049699542683 1102 18-19 0.2221822752958147 1102 20-21 0.11424146684824876 1102 22-23 0.27376845674496764 1102 24-25 0.1447107531125198 1102 26-27 0.2275725551168648 1102 28-29 0.07907035898559656 1102 30-31 0.18478008860699902 1102 32-33 0.3288517908035047 1102 34-35 0.1984077355282423 1102 36-37 0.19180919028924137 1102 38-39 0.3067118566384863 1102 40-41 0.22385577399833068 1102 42-43 0.24115540911939348 1102 44-45 0.3616557825624085 1102 46-47 0.4837720678469566 1102 48-49 0.5793892511752432 1102 50-51 0.5902477304152924 1102 52-53 0.7053357640686073 1102 54-55 0.5532037682431152 1102 56-57 0.7152052464295693 1102 58-59 0.49618395037594354 1102 60-61 0.5369924930401169 1102 62-63 0.3964267427297159 1102 64-65 0.5733399065406743 1102 66-67 0.269162666627345 1102 68-69 0.3517161470817882 1102 70-71 0.45611150382254806 1102 72-73 0.41383941476361485 1102 74-75 0.6468592462248068 1102 76-77 0.5483862230163261 1102 78-79 0.36593728729312147 1102 80-81 0.36971254556198474 1102 82-83 0.44217470329553876 1102 84-85 0.3549729944687279 1102 86-87 0.4343985268006527 1102 88-89 0.5713447359499142 1102 90-91 0.39244049554097415 1102 92-93 0.46095403500206444 1102 94-95 0.6095777014440991 1102 96-97 0.43349734436432286 1102 98-99 0.6075939394943077 1102 100-101 0.6745411332427551 1103 1 0.3906118305057227 1103 2 0.3140281815796868 1103 3 0.37290094303875065 1103 4 0.32934046161611974 1103 5 0.2181521711828296 1103 6 0.40581338954562796 1103 7 0.43192319510902166 1103 8 0.35834490261525076 1103 9 0.28539480093805025 1103 10-11 0.39408971987877095 1103 12-13 0.3816179145693681 1103 14-15 0.6079156005904593 1103 16-17 0.6319753614343142 1103 18-19 0.6064576248682911 1103 20-21 0.4755080831055096 1103 22-23 0.587295101009154 1103 24-25 0.5737650741960678 1103 26-27 0.6827239660852555 1103 28-29 0.6756760051435577 1103 30-31 0.6701124365963693 1103 32-33 0.6733836458163083 1103 34-35 0.6879394077879226 1103 36-37 0.5524844288391932 1103 38-39 0.8019934563680096 1103 40-41 0.6357133681904372 1103 42-43 0.7647557485833332 1103 44-45 0.7835217948205795 1103 46-47 0.7007032887064 1103 48-49 0.6806258616529348 1103 50-51 0.7210298681243401 1103 52-53 0.7832280607957287 1103 54-55 0.7784443170507629 1103 56-57 0.7943542564787052 1103 58-59 0.8332563937167592 1103 60-61 0.7721102774325459 1103 62-63 0.844449239640042 1103 64-65 0.796655436673241 1103 66-67 0.7692897942735542 1103 68-69 0.8923524477929305 1103 70-71 0.8359106010220856 1103 72-73 0.8117980622990757 1103 74-75 0.6533887871260973 1103 76-77 0.8076442110414241 1103 78-79 0.9092496078238383 1103 80-81 0.8379713536917563 1103 82-83 0.8275577081681966 1103 84-85 0.7759566517700556 1103 86-87 0.8534007403979409 1103 88-89 0.7762080251864418 1103 90-91 0.9191684263871913 1103 92-93 0.8693687886622676 1103 94-95 1.0610213940624718 1103 96-97 1.123545865320395 1103 98-99 0.9794533269515036 1103 100-101 1.0452377539089355 1104 1 0.1991024306953264 1104 2 0.14588198845198974 1104 3 0.12614115689603267 1104 4 0.2535505449462434 1104 5 0.28066322945056754 1104 6 0.12781408188934762 1104 7 0.10008986441534518 1104 8 0.19396270625661316 1104 9 0.3435589135502397 1104 10-11 0.3491072321022628 1104 12-13 0.1825942097159512 1104 14-15 0.052679920905760014 1104 16-17 0.3192581838436297 1104 18-19 0.42015053196526253 1104 20-21 0.3940789589655651 1104 22-23 0.23379667235789015 1104 24-25 0.16599543912455772 1104 26-27 0.1462933939061557 1104 28-29 0.22555908729489005 1104 30-31 0.31429018792731966 1104 32-33 0.08633233997912981 1104 34-35 0.09267879467909523 1104 36-37 0.15954107432074238 1104 38-39 0.3096356526813935 1104 40-41 0.30826137408214294 1104 42-43 0.3107811639330009 1104 44-45 0.3641333990443272 1104 46-47 0.2012914980476026 1104 48-49 0.35954823770417477 1104 50-51 0.3187753390425101 1104 52-53 0.4348364589702953 1104 54-55 0.5393793317687212 1104 56-57 0.5376628449346654 1104 58-59 0.37717468584508396 1104 60-61 0.38413539412413655 1104 62-63 0.22070252883835195 1104 64-65 0.25126744137032375 1104 66-67 0.06957250129224946 1104 68-69 0.055948064759419935 1104 70-71 0.14087268049680546 1104 72-73 0.12007089097568269 1104 74-75 0.17492972703926313 1104 76-77 0.3142236823713809 1104 78-79 0.37869818772731634 1104 80-81 0.21280482918935206 1104 82-83 0.12889731407529226 1104 84-85 0.17376858542271734 1104 86-87 0.2944499352655434 1104 88-89 0.31652777033155033 1104 90-91 0.32532276247255254 1104 92-93 0.4277622118494442 1104 94-95 0.5972024057440741 1104 96-97 0.537636078159526 1104 98-99 0.5585208463020592 1104 100-101 0.49582913083578717 1105 1 0.10572361325162305 1105 2 0.14247389027785218 1105 3 0.1345570166420238 1105 4 0.16213080785372824 1105 5 0.20584717984988288 1105 6 0.1074690186847036 1105 7 0.1830632457779373 1105 8 0.1646274040597575 1105 9 0.17569881444975977 1105 10-11 0.14219519123306412 1105 12-13 0.17707437930113912 1105 14-15 0.3165015377432212 1105 16-17 0.39571597212812293 1105 18-19 0.24979732885513073 1105 20-21 0.29819798043427426 1105 22-23 0.2534869317571875 1105 24-25 0.12806193183418202 1105 26-27 0.3962178991489438 1105 28-29 0.32720162850459644 1105 30-31 0.36518110631502765 1105 32-33 0.18560863359353874 1105 34-35 0.08291807350639857 1105 36-37 0.08385915660486631 1105 38-39 0.3211976432207564 1105 40-41 0.3393935317532595 1105 42-43 0.40654205019674095 1105 44-45 0.30100034942299914 1105 46-47 0.23083018323504234 1105 48-49 0.21250950092579046 1105 50-51 -0.041716253925521585 1105 52-53 0.13307583180777272 1105 54-55 0.06922612957576746 1105 56-57 0.18173814020686763 1105 58-59 0.18489980009037055 1105 60-61 0.23258296510755372 1105 62-63 0.08675000856310788 1105 64-65 0.21281504376688787 1105 66-67 0.04019294552472985 1105 68-69 -0.031113039562065126 1105 70-71 0.04228088414577513 1105 72-73 0.20578335053411223 1105 74-75 0.2801244198208863 1105 76-77 0.3181202321979235 1105 78-79 0.3060672441632306 1105 80-81 -0.031141718000320395 1105 82-83 0.075689847374381 1105 84-85 0.0993376909273529 1105 86-87 0.13797005458968314 1105 88-89 0.16220295829457854 1105 90-91 0.19522402885840862 1105 92-93 0.2923975899423219 1105 94-95 0.2945606624743995 1105 96-97 0.2714879960817633 1105 98-99 0.43647586128636107 1105 100-101 0.5585261222808597 1106 1 0.2613244383688169 1106 2 0.29260147060212915 1106 3 0.3654302330837531 1106 4 0.30781800631690004 1106 5 0.37797307865571383 1106 6 0.3048106064487257 1106 7 0.37770682741223993 1106 8 0.3740385304327134 1106 9 0.3690531504699379 1106 10-11 0.37375176895196205 1106 12-13 0.4754372206762625 1106 14-15 0.4186729695268525 1106 16-17 0.5668031422401043 1106 18-19 0.6093321250443395 1106 20-21 0.5666475128841668 1106 22-23 0.3239206804190573 1106 24-25 0.20817394344839357 1106 26-27 0.2483472929713173 1106 28-29 0.24177853597709031 1106 30-31 0.37586143694847607 1106 32-33 0.35639481679106666 1106 34-35 0.45535546871700916 1106 36-37 0.15499801874479857 1106 38-39 0.02491409228395014 1106 40-41 0.21128626651816518 1106 42-43 0.22800548450264557 1106 44-45 0.22583415283198605 1106 46-47 0.25669574974545384 1106 48-49 0.1859939346597912 1106 50-51 0.15775179477984835 1106 52-53 0.26102032214637916 1106 54-55 0.2120128309156044 1106 56-57 0.2596919180020265 1106 58-59 0.27334082718531505 1106 60-61 0.3925960653094336 1106 62-63 0.49441668174728903 1106 64-65 0.18640090630275807 1106 66-67 0.1254427536654248 1106 68-69 0.2383644676845904 1106 70-71 0.3433073925020409 1106 72-73 0.3396555600200273 1106 74-75 0.4991205679704791 1106 76-77 0.548538974285254 1106 78-79 0.36565471000738725 1106 80-81 0.50529468491888 1106 82-83 0.39973409477267197 1106 84-85 0.34586821488654707 1106 86-87 0.5713681149777017 1106 88-89 0.4728630031676566 1106 90-91 0.3867751318660808 1106 92-93 0.5860883518559312 1106 94-95 0.7507836249775188 1106 96-97 0.8163327244725416 1106 98-99 0.9420538343722882 1106 100-101 1.3492728826693714 1107 1 0.36558595551097284 1107 2 0.4384819659983705 1107 3 0.389985973582359 1107 4 0.35006466504915323 1107 5 0.389135495088361 1107 6 0.3751489888450905 1107 7 0.502705570964622 1107 8 0.36809600863232816 1107 9 0.4244839517194876 1107 10-11 0.5072155057005148 1107 12-13 0.5333572823549417 1107 14-15 0.4440232603299492 1107 16-17 0.48350501207865193 1107 18-19 0.6205441202206643 1107 20-21 0.6822946187216061 1107 22-23 0.6776747285574345 1107 24-25 0.6807519403204481 1107 26-27 0.6580768293788211 1107 28-29 0.620134371721214 1107 30-31 0.7277333944775606 1107 32-33 0.7008913589113988 1107 34-35 0.6833967714779519 1107 36-37 0.5790825030973394 1107 38-39 0.7867748219254906 1107 40-41 0.9634248082728334 1107 42-43 0.8505199934163628 1107 44-45 0.689705918566041 1107 46-47 0.803494144643814 1107 48-49 0.8660556459332867 1107 50-51 0.7684292767664687 1107 52-53 0.8040571845535425 1107 54-55 0.8650034614838731 1107 56-57 0.7936450716583678 1107 58-59 0.894891063492544 1107 60-61 0.9417318602658753 1107 62-63 0.956427818355607 1107 64-65 0.9803299823478611 1107 66-67 0.9687375885383247 1107 68-69 0.97707545566535 1107 70-71 1.0504190625776388 1107 72-73 0.9784552599939218 1107 74-75 0.9607029503201261 1107 76-77 0.9276002544274284 1107 78-79 0.8697131716319433 1107 80-81 1.0430169776681808 1107 82-83 1.0222476528611644 1107 84-85 0.9608502863978394 1107 86-87 1.0184987689580076 1107 88-89 1.025721278865138 1107 90-91 0.9820777021096525 1107 92-93 1.1283920601014614 1107 94-95 1.2499904329947675 1107 96-97 1.121653841241212 1107 98-99 1.4690775775647253 1107 100-101 1.4616092150865612 1108 1 -0.01935970948390775 1108 2 0.09945284423859135 1108 3 0.20428212959861725 1108 4 0.024545115609164725 1108 5 0.1806284051799949 1108 6 0.05993594753704201 1108 7 0.08048066088952766 1108 8 -0.0034894421297124723 1108 9 -0.030804438076316387 1108 10-11 -0.10604873262591497 1108 12-13 0.0628222323459866 1108 14-15 0.175436146492288 1108 16-17 0.2994779126540408 1108 18-19 0.1809746689102738 1108 20-21 0.2908132705461668 1108 22-23 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0.5020887034361863 2203 2 0.4744452793997773 2203 3 0.5808906470950426 2203 4 0.4397148752474678 2203 5 0.449522462987467 2203 6 0.4824039145180379 2203 7 0.5469243161079191 2203 8 0.5212230241660194 2203 9 0.7091780859821952 2203 10-11 0.542198249818064 2203 12-13 0.4652284795528132 2203 14-15 0.8360692722929883 2203 16-17 0.749445698078901 2203 18-19 0.7840298861817558 2203 20-21 0.8624870341954605 2203 22-23 0.8881651686140088 2203 24-25 0.9258497009896658 2203 26-27 0.9212410570068883 2203 28-29 0.8938855543295361 2203 30-31 0.6655238863066373 2203 32-33 0.7279540876623329 2203 34-35 0.8720548534280965 2203 36-37 0.9109005436294808 2203 38-39 0.9640549733382073 2203 40-41 0.7833510785933058 2203 42-43 0.663134611717993 2203 44-45 0.9036025498564513 2203 46-47 0.85722620882229 2203 48-49 0.9896371837417917 2203 50-51 1.139724033756238 2203 52-53 0.9866264120650428 2203 54-55 0.9754531901881833 2203 56-57 0.883571324980359 2203 58-59 1.028237983465658 2203 60-61 1.0004887956444648 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0.7224267416696684 2204 88-89 0.6140988985713669 2204 90-91 0.518350993293641 2204 92-93 0.6195705786017527 2204 94-95 0.6659219914299008 2204 96-97 0.7423372019786854 2204 98-99 0.9164802668816847 2204 100-101 0.8444852050403 2205 1 0.422380201401495 2205 2 0.525388486986369 2205 3 0.4775339824493692 2205 4 0.48163395168887746 2205 5 0.525099280337848 2205 6 0.49078042940485744 2205 7 0.5576027053781658 2205 8 0.4920660556866281 2205 9 0.681298901845949 2205 10-11 0.486033649528963 2205 12-13 0.6167941266467025 2205 14-15 0.7971065908319872 2205 16-17 0.7861772077248546 2205 18-19 0.7655812792953114 2205 20-21 0.7595763684159849 2205 22-23 0.7513999615138189 2205 24-25 0.7530120542109131 2205 26-27 0.7470165480391628 2205 28-29 0.6354509680674809 2205 30-31 0.6137954573872904 2205 32-33 0.662983779529732 2205 34-35 0.5909296667433424 2205 36-37 0.37485839740565297 2205 38-39 0.3548190235067352 2205 40-41 0.5539614392545431 2205 42-43 0.6617387578322038 2205 44-45 0.6635628343879887 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0.46719368393409155 2206 72-73 0.4100284973912949 2206 74-75 0.43456802233225034 2206 76-77 0.38121289294700134 2206 78-79 0.4218733349476693 2206 80-81 0.43577512306677235 2206 82-83 0.6500914735079881 2206 84-85 0.8007359012181823 2206 86-87 0.6972968689554655 2206 88-89 0.7953919602430553 2206 90-91 0.7326407376217325 2206 92-93 0.8824417312908608 2206 94-95 0.8283853238970273 2206 96-97 0.8386850805877906 2206 98-99 0.9520630494704321 2206 100-101 1.0015356722074955 2207 1 0.41795110730859264 2207 2 0.5114410124343003 2207 3 0.46228647379235 2207 4 0.46344364944052785 2207 5 0.440744065531149 2207 6 0.5117464122049071 2207 7 0.5085565844536859 2207 8 0.5252912643295318 2207 9 0.5328368026883012 2207 10-11 0.749088128739011 2207 12-13 0.7648646593626083 2207 14-15 0.967112026794112 2207 16-17 0.9583089655828516 2207 18-19 0.9493278722423071 2207 20-21 0.9534580200626195 2207 22-23 0.9815224580054647 2207 24-25 0.9603064426244785 2207 26-27 0.9993680715151285 2207 28-29 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11248.0 13 13840.0 14 15288.0 15 18318.0 16 22674.0 17 29080.0 18 25791.0 19 17351.0 20 17191.0 21 18312.0 22 19696.0 23 21686.0 24 24769.0 25 28767.0 26 33405.0 27 39018.0 28 46421.0 29 55687.0 30 67409.0 31 83034.0 32 103358.0 33 133058.0 34 176992.0 35 245517.0 36 357246.0 37 502892.0 38 435165.0 39 75968.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.924164763287685 18.874940086855904 14.412970362406089 22.787924787450322 2 15.223381147212853 20.40741164164131 40.532028869310174 23.83717834183566 3 18.81826177817068 25.334713122720576 30.01027022075389 25.836754878354856 4 12.478769368295591 16.787321215733016 37.68966030989273 33.04424910607867 5 13.561252159888983 37.52630341404993 33.97728488027405 14.93515954578704 6 31.00967756122178 36.47303096327625 17.821084009102854 14.696207466399116 7 27.763273318520532 33.314907351705926 21.108538840764876 17.813280489008665 8 26.141270550396467 34.53696193013787 20.075198531650116 19.246568987815543 9 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29.905632331141852 21.987110239821916 34-35 22.018582769686017 26.47489937373624 29.678223065464497 21.828294791113244 36-37 22.029391161802174 26.260809283590664 29.63777905231439 22.07202050229277 38-39 21.73518246120752 26.61686068957712 29.737347744230036 21.910609104985326 40-41 21.990854503051477 26.37240162745466 29.74524109501726 21.8915027744766 42-43 21.836927530793044 26.47178178969738 29.45285473247168 22.238435947037896 44-45 21.963888455061237 26.69602464745014 29.205165897659324 22.1349209998293 46-47 22.099280598841386 26.844383404872612 28.798849315547887 22.25748668073812 48-49 22.163715007900894 27.080064562280803 28.440406933725377 22.315813496092925 50-51 22.12456113473836 27.20330790997103 28.33180661615501 22.340324339135602 52-53 22.39375964746766 27.195024605929103 28.11516870456429 22.296047042038943 54-55 22.088009344016253 27.13730037013415 28.242304013392 22.532386272457604 56-57 22.14292495769551 27.549355240451472 28.028713068504423 22.2790067333486 58-59 22.277577818793414 27.1238017585842 28.13576485940355 22.462855563218838 60-61 22.343071404225963 27.023086229032085 28.46489355979075 22.168948806951203 62-63 22.1909289286673 27.128437282543256 28.488308669154495 22.19232511963495 64-65 22.06488758502325 27.11872168218523 28.38886758524663 22.42752314754489 66-67 21.97188364183676 27.485884010853024 28.01693602185202 22.525296325458193 68-69 21.93627348280462 27.326901348988503 28.326877697879237 22.409947470327634 70-71 22.015617707021413 27.300218251551396 28.158193820340866 22.525970221086325 72-73 22.109326324658056 27.303230504639885 28.04362390308728 22.54381926761478 74-75 21.9905350912794 27.51004389900622 27.883315771248572 22.616105238465806 76-77 22.129351318260827 27.322074631689798 28.00943061796874 22.539143432080635 78-79 22.121039131658797 27.44135315610807 27.87182970407344 22.56577800815969 80-81 22.02252792145592 27.63910244492738 27.748005157882854 22.590364475733846 82-83 22.135075984989573 27.60570104340877 27.794001712264365 22.465221259337294 84-85 22.103067211927275 27.459290221704023 27.629797411246997 22.807845155121704 86-87 22.068735656796527 27.450505765211357 27.774139640541286 22.70661893745083 88-89 22.126950788751746 27.386419357403884 27.656490964929613 22.830138888914757 90-91 21.984105923267087 27.441463335149002 27.902326382584487 22.67210435899943 92-93 22.063374477320156 27.277007003081238 27.84882164023984 22.81079687935877 94-95 21.98370084254759 27.11795777990999 27.85005341086752 23.048287966674895 96-97 21.912677477026634 27.213898087419334 27.91361318586145 22.95981124969258 98-99 21.745231713927602 27.19342462814845 27.751651189029282 23.309692468894667 100-101 21.711968159272192 27.24182824881027 27.876545275071678 23.16965831684587 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 558.0 1 416.5 2 393.5 3 539.0 4 473.5 5 490.5 6 889.0 7 1921.5 8 3284.0 9 3970.5 10 3593.0 11 3178.0 12 3373.0 13 3627.5 14 3905.0 15 4153.5 16 4246.0 17 4433.5 18 4771.5 19 5166.5 20 5723.5 21 6571.0 22 7755.0 23 9412.0 24 11339.0 25 13774.0 26 17178.0 27 21196.5 28 24594.5 29 28161.0 30 32923.0 31 37897.5 32 42980.0 33 48465.0 34 55211.0 35 59665.5 36 65084.0 37 72221.0 38 75632.5 39 76551.0 40 77802.5 41 81462.0 42 84690.0 43 87922.5 44 91128.5 45 93693.5 46 94616.5 47 96371.5 48 102970.0 49 103630.0 50 98468.0 51 94937.0 52 91490.5 53 90280.0 54 87929.5 55 80922.0 56 73381.0 57 66302.5 58 60326.5 59 54373.0 60 47273.0 61 39073.5 62 31028.0 63 24409.5 64 18478.5 65 13832.5 66 10010.0 67 7682.5 68 5499.0 69 3312.0 70 2208.5 71 1498.5 72 1038.0 73 815.5 74 598.5 75 441.5 76 303.0 77 153.0 78 89.5 79 52.0 80 35.0 81 28.5 82 21.5 83 15.0 84 10.0 85 9.0 86 8.0 87 6.0 88 6.0 89 6.0 90 5.5 91 4.5 92 2.0 93 1.0 94 2.0 95 2.5 96 2.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03581401876848173 2 0.028144904562341148 3 0.024757091976133416 4 0.04836009395037189 5 0.09351851886322879 6 0.11112769856956128 7 0.055247405252002994 8 0.08450917110650054 9 0.06876142688709537 10-11 0.07015750350229087 12-13 0.06771902301441607 14-15 0.06224640268284974 16-17 0.05031460121164558 18-19 0.06029189542157605 20-21 0.057350827352230876 22-23 0.033152166022175654 24-25 0.009083805176205346 26-27 0.0030713685534300857 28-29 0.006403338075029997 30-31 0.0047280461367954045 32-33 0.006273037590945084 34-35 0.006328880655552904 36-37 0.0057890643643439795 38-39 0.0028293819401295334 40-41 0.006961768721108194 42-43 0.0038345571030702886 44-45 0.002252336939182063 46-47 0.0016939062931038654 48-49 3.9090145225473814E-4 50-51 0.001954507261273691 52-53 0.005993822267905986 54-55 0.011764272277380692 56-57 0.00796694388404895 58-59 0.004895575330618864 60-61 0.002252336939182063 62-63 0.008190316142480229 64-65 0.002568780971959708 66-67 0.02161126600322624 68-69 0.045996070881974195 70-71 0.05889581880638055 72-73 0.0035181130702926435 74-75 0.014481968088294586 76-77 0.02200216745548098 78-79 0.026171782946198187 80-81 0.0763933123834974 82-83 0.028517191659726614 84-85 0.010740482759570664 86-87 0.03021109795283048 88-89 0.04674064507674512 90-91 0.042310428617858095 92-93 0.04236627168246591 94-95 0.011764272277380692 96-97 0.01617587438139845 98-99 0.03680057957655321 100-101 0.07406651802483824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2686099.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.36170237195281 #Duplication Level Percentage of deduplicated Percentage of total 1 73.94629369426052 37.9800752823362 2 14.010403383384626 14.391963373768036 3 5.018607006673223 7.732925981956412 4 2.287568916198572 4.69973735316487 5 1.2568061547086806 3.2275851828692868 6 0.786635643747584 2.4241767485599746 7 0.5178931528822147 1.8619911785165995 8 0.35516149405408987 1.4593359161267403 9 0.26323337134652747 1.216810266611947 >10 1.3815575920588752 12.972376927008206 >50 0.11110196512479469 3.940222036294437 >100 0.06103189734307948 5.660312958025812 >500 0.0030517761787615246 1.0196597708915565 >1k 4.359680255306789E-4 0.3116228345963422 >5k 1.4532267517689294E-4 0.6052200609160338 >10k+ 7.266133758844647E-5 0.4959841283575315 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13290 0.49476955242528287 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9793 0.3645807544695858 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6424 0.23915723136042266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.8614354869273247E-5 1.8614354869273247E-5 12-13 0.0 0.0 0.0 3.7228709738546495E-5 1.1168612921563948E-4 14-15 0.0 0.0 0.0 3.7228709738546495E-5 1.1168612921563948E-4 16-17 0.0 0.0 0.0 7.445741947709299E-5 1.4891483895418598E-4 18-19 0.0 0.0 0.0 9.307177434636623E-5 1.4891483895418598E-4 20-21 0.0 0.0 0.0 2.2337225843127898E-4 1.4891483895418598E-4 22-23 0.0 0.0 0.0 4.2813016199328465E-4 1.4891483895418598E-4 24-25 0.0 0.0 0.0 6.701167752938368E-4 1.4891483895418598E-4 26-27 0.0 0.0 0.0 0.0010237895178100286 1.675291938234592E-4 28-29 0.0 0.0 0.0 0.0019917359710122373 1.8614354869273247E-4 30-31 0.0 0.0 0.0 0.0036297991995082836 1.8614354869273247E-4 32-33 0.0 0.0 0.0 0.006403338075029998 1.8614354869273247E-4 34-35 0.0 0.0 0.0 0.008748746788558425 1.8614354869273247E-4 36-37 0.0 0.0 0.0 0.011131384211825402 1.8614354869273247E-4 38-39 0.0 0.0 0.0 0.014575039862640953 1.8614354869273247E-4 40-41 0.0 0.0 0.0 0.01907971374100508 1.8614354869273247E-4 42-43 0.0 0.0 0.0 0.024943235524826152 1.8614354869273247E-4 44-45 0.0 0.0 0.0 0.030285555372307572 1.8614354869273247E-4 46-47 0.0 0.0 0.0 0.03693088006063812 2.0475790356200573E-4 48-49 0.0 0.0 0.0 0.042626872650635736 2.2337225843127896E-4 50-51 0.0 0.0 0.0 0.05109640411615506 2.6060096816982544E-4 52-53 0.0 0.0 0.0 0.07496000705856337 3.350583876469185E-4 54-55 0.0 0.0 0.0 0.11105324115008419 3.350583876469185E-4 56-57 0.0 0.0 0.0 0.13523328812527013 3.350583876469185E-4 58-59 0.0 0.0 0.0 0.17322518641345683 3.350583876469185E-4 60-61 0.0 0.0 0.0 0.2192398716503003 3.7228709738546494E-4 62-63 0.0 0.0 0.0 0.24798043556845822 3.7228709738546494E-4 64-65 0.0 0.0 0.0 0.26882851302204425 3.7228709738546494E-4 66-67 0.0 0.0 0.0 0.3379436126516558 4.0951580712401145E-4 68-69 0.0 0.0 0.0 0.4130897632589119 4.0951580712401145E-4 70-71 0.0 0.0 0.0 0.441774484112462 4.467445168625579E-4 72-73 0.0 0.0 0.0 0.5029970972775016 4.8397322660110437E-4 74-75 0.0 0.0 0.0 0.5913035967773339 5.212019363396509E-4 76-77 0.0 0.0 0.0 0.6734859735251754 5.212019363396509E-4 78-79 0.0 0.0 0.0 0.7550913052720692 5.212019363396509E-4 80-81 0.0 0.0 0.0 0.8760101545028682 5.212019363396509E-4 82-83 0.0 0.0 0.0 1.0101824244005897 5.212019363396509E-4 84-85 0.0 0.0 0.0 1.133781740732564 5.212019363396509E-4 86-87 0.0 0.0 0.0 1.35108571947646 5.212019363396509E-4 88-89 0.0 0.0 0.0 1.6223154842766405 5.212019363396509E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7480 0.0 42.6381 1 GTATCAA 13310 0.0 40.31743 1 ATCAACG 16745 0.0 31.850554 3 TCAACGC 17020 0.0 31.3371 4 TATCAAC 17145 0.0 31.246094 2 CAACGCA 17340 0.0 30.840462 5 AACGCAG 17855 0.0 30.081783 6 ACGCAGA 19935 0.0 26.748343 7 CGCAGAG 20020 0.0 26.420635 8 GTACATG 12525 0.0 25.42576 1 TACATGG 12815 0.0 24.815145 2 GCAGAGT 20955 0.0 24.265207 9 ACATGGG 12890 0.0 23.711956 3 GAGTACT 11265 0.0 22.760008 12-13 AGTACTT 11780 0.0 21.724642 12-13 AGAGTAC 18035 0.0 21.566833 10-11 GTACTTT 12050 0.0 21.237467 14-15 CATGGGG 5600 0.0 20.797136 4 CAGAGTA 20400 0.0 19.509155 10-11 TACTTTT 13510 0.0 18.766495 14-15 >>END_MODULE