FastQCFastQC Report
Thu 26 May 2016
SRR936952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686099
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134480.5006516885639732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108540.40408041550218365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73350.27307258593223854No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.10234172307126432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82500.042.9594461
GTATCAA141300.037.522851
ATCAACG181150.028.9740223
TCAACGC183350.028.6522714
TATCAAC185550.028.3642792
CAACGCA186250.028.2571475
AACGCAG191250.027.4961226
ACGCAGA216500.024.135717
CGCAGAG216850.023.9890088
GTACATG125100.022.8999121
TACATGG126900.022.1965772
GCAGAGT230200.022.164439
GAGTACT129750.021.61971512-13
AGTACTT135200.021.57392112-13
ACATGGG127850.021.3996453
AGAGTAC199900.020.8778910-11
GTACTTT140400.019.91023314-15
CATGGGG54200.019.1924174
GTATAGG8700.019.1127221
TACTTTT152900.018.95044114-15
Produced by FastQC (version 0.11.5)