##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936952_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2686099 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03979414012663 31.0 31.0 34.0 30.0 34.0 2 32.16523739445196 33.0 31.0 34.0 30.0 34.0 3 32.29349216093674 34.0 31.0 34.0 30.0 34.0 4 35.995060494791886 37.0 35.0 37.0 35.0 37.0 5 35.819756084939534 37.0 35.0 37.0 35.0 37.0 6 35.90554145621587 37.0 35.0 37.0 35.0 37.0 7 35.85928292293024 37.0 35.0 37.0 35.0 37.0 8 35.79981676029067 37.0 35.0 37.0 35.0 37.0 9 37.479789464200685 39.0 37.0 39.0 35.0 39.0 10-11 37.39239395122816 39.0 37.0 39.0 34.0 39.0 12-13 37.277103338335635 39.0 37.0 39.0 34.0 39.0 14-15 38.4413055885133 40.0 38.0 41.0 34.0 41.0 16-17 38.4366722894428 40.0 38.0 41.0 33.5 41.0 18-19 38.565040417348726 40.0 38.0 41.0 34.0 41.0 20-21 38.5690447373682 40.0 38.0 41.0 34.0 41.0 22-23 38.47868637753113 40.0 38.0 41.0 34.0 41.0 24-25 38.38106376570633 40.0 38.0 41.0 34.0 41.0 26-27 38.17830802215406 40.0 38.0 41.0 33.5 41.0 28-29 38.014998888723014 40.0 37.0 41.0 33.0 41.0 30-31 37.990008372736824 40.0 37.0 41.0 33.0 41.0 32-33 37.852702562340404 40.0 37.0 41.0 33.0 41.0 34-35 37.71915927149372 40.0 37.0 41.0 33.0 41.0 36-37 37.55981164506595 40.0 37.0 41.0 33.0 41.0 38-39 37.392884067191865 40.0 36.0 41.0 32.5 41.0 40-41 37.06324953026676 39.5 36.0 41.0 31.5 41.0 42-43 36.84210094266816 39.0 36.0 41.0 31.0 41.0 44-45 36.58983492417815 39.0 35.5 41.0 30.5 41.0 46-47 36.189748776943816 39.0 35.0 40.0 30.0 41.0 48-49 35.799375972367365 39.0 35.0 40.0 28.5 41.0 50-51 35.50540709780243 39.0 35.0 40.0 28.0 41.0 52-53 35.23268446174173 38.0 34.5 40.0 27.0 41.0 54-55 34.83484469485302 38.0 34.0 40.0 26.0 41.0 56-57 34.625547680856144 38.0 34.0 40.0 26.0 41.0 58-59 34.30021008160905 37.5 33.5 40.0 25.5 41.0 60-61 33.92012710626079 37.0 33.0 40.0 24.5 41.0 62-63 33.533482012390465 36.5 33.0 39.0 23.5 40.5 64-65 33.207551173653684 36.0 32.0 39.0 23.0 40.0 66-67 32.86996886563004 36.0 32.0 39.0 22.0 40.0 68-69 32.419449543743546 35.0 31.5 38.5 21.5 40.0 70-71 31.97836527990964 35.0 31.0 38.0 20.0 40.0 72-73 31.566457528184927 35.0 31.0 37.0 19.5 39.0 74-75 31.07988983280214 34.0 30.5 36.5 18.0 39.0 76-77 29.657101059938597 33.0 28.5 35.0 16.0 37.5 78-79 30.346024290243953 34.0 30.0 36.0 16.5 37.5 80-81 30.215629245236308 34.0 30.0 35.0 13.0 37.0 82-83 29.866754911118317 34.0 30.0 35.0 8.5 37.0 84-85 29.420856602828117 34.0 29.0 35.0 7.0 36.0 86-87 29.029775894335987 34.0 29.0 35.0 2.0 36.0 88-89 28.639521663200053 33.5 29.0 35.0 2.0 35.5 90-91 28.169166326334214 33.0 28.0 35.0 2.0 35.0 92-93 27.65706904324822 33.0 27.0 35.0 2.0 35.0 94-95 27.06899559547135 32.5 26.0 34.0 2.0 35.0 96-97 26.259756621032956 32.0 25.0 34.0 2.0 35.0 98-99 25.356289362380167 32.0 22.0 34.0 2.0 35.0 100-101 22.172563073810757 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1675456206759094 1101 2 0.23707332498041467 1101 3 0.27635470880259305 1101 4 0.15144530402363898 1101 5 -0.002662493082013384 1101 6 0.1341734569202444 1101 7 0.13454183978542744 1101 8 0.19405314387402228 1101 9 0.21219847258463886 1101 10-11 0.16770223963254693 1101 12-13 0.18787223600622127 1101 14-15 0.28094643795162 1101 16-17 0.3704701175543832 1101 18-19 0.1415031183681421 1101 20-21 0.04147836967903373 1101 22-23 0.11880805168470232 1101 24-25 0.09256306281206861 1101 26-27 0.09404416620283484 1101 28-29 0.21893711284332795 1101 30-31 0.33777324765149075 1101 32-33 0.3151645590660266 1101 34-35 0.08761668046131632 1101 36-37 0.22474694180999677 1101 38-39 0.30194765022268655 1101 40-41 0.3021257921184528 1101 42-43 0.26930236542083463 1101 44-45 0.09823707486245326 1101 46-47 -0.022334993481798904 1101 48-49 0.10839865844224761 1101 50-51 0.18667852588254874 1101 52-53 0.29048326105264266 1101 54-55 0.09551116929645076 1101 56-57 0.1705986517650686 1101 58-59 0.2552734560125671 1101 60-61 0.23218152332239583 1101 62-63 0.3722163505353606 1101 64-65 0.28514676807110817 1101 66-67 0.31086037127543165 1101 68-69 -0.17676363884201862 1101 70-71 -0.34758724843011635 1101 72-73 0.04030093693758019 1101 74-75 -0.02747558256800886 1101 76-77 -0.028786274270860446 1101 78-79 0.08570538986614551 1101 80-81 0.059860469078802936 1101 82-83 0.10185869311228757 1101 84-85 -0.04098865748281 1101 86-87 -0.4560386963380303 1101 88-89 -0.13893886871360195 1101 90-91 0.10408141257255465 1101 92-93 -0.01903421277651063 1101 94-95 -0.12508760432490718 1101 96-97 -0.31832472825954383 1101 98-99 -0.10550310282315323 1101 100-101 0.3139322211589608 1102 1 -0.1263170480761886 1102 2 -0.05087610963803968 1102 3 0.0796494207249836 1102 4 0.05590468302537488 1102 5 -0.13204177833389252 1102 6 0.06370005324301786 1102 7 0.0678331967483885 1102 8 0.18295283531226403 1102 9 0.20614903182330124 1102 10-11 0.26882231174054994 1102 12-13 0.25014264573655254 1102 14-15 0.2681655191652581 1102 16-17 0.2522333731881119 1102 18-19 0.25190414233956204 1102 20-21 0.24335248073291638 1102 22-23 0.2061612131433037 1102 24-25 0.08631935336099872 1102 26-27 0.19750887061200473 1102 28-29 -0.011417000762207863 1102 30-31 0.004534282396583933 1102 32-33 -0.06459712068183165 1102 34-35 0.2308035138993887 1102 36-37 0.12871713464928547 1102 38-39 0.24893710572694516 1102 40-41 0.2649215365177966 1102 42-43 0.12805768728430422 1102 44-45 0.15707113195345812 1102 46-47 0.029936455813420082 1102 48-49 0.010599169246638951 1102 50-51 0.03434824666486236 1102 52-53 0.08198416830927613 1102 54-55 0.365157882617531 1102 56-57 0.24889962470330573 1102 58-59 -0.051279878391831346 1102 60-61 0.051731958758331587 1102 62-63 -9.646471634141562E-4 1102 64-65 0.09042947065631779 1102 66-67 -0.01885559124352909 1102 68-69 0.02754464885284591 1102 70-71 0.019975314475374262 1102 72-73 -0.10409167364613481 1102 74-75 0.1433659215878862 1102 76-77 0.22274891500082816 1102 78-79 -0.13531579363123214 1102 80-81 0.033483813453575095 1102 82-83 0.1738781542396488 1102 84-85 0.08429793165752031 1102 86-87 0.03261882373265834 1102 88-89 0.09697230991672257 1102 90-91 0.0762033535347193 1102 92-93 0.010761134698054065 1102 94-95 -0.037735328859117345 1102 96-97 0.23121248816369544 1102 98-99 0.18484453712827076 1102 100-101 0.12793049406364787 1103 1 0.154801812275295 1103 2 0.2247513618124941 1103 3 0.219083548501402 1103 4 0.11355861256033961 1103 5 -0.04591296935888778 1103 6 0.02976222585792243 1103 7 0.11528664609949857 1103 8 0.17778035062355002 1103 9 0.24833427953375065 1103 10-11 0.20678756123283648 1103 12-13 0.21789128708125105 1103 14-15 0.19595256151144724 1103 16-17 0.1824983766490007 1103 18-19 0.18825876565109212 1103 20-21 0.24110301814133805 1103 22-23 0.25663237355714585 1103 24-25 0.268841072428323 1103 26-27 0.265261167019311 1103 28-29 0.1989380200373816 1103 30-31 0.37414152307560045 1103 32-33 0.36644823864508425 1103 34-35 0.31541764208700585 1103 36-37 0.3617627269864485 1103 38-39 0.29774167181390254 1103 40-41 0.42053502735387127 1103 42-43 0.24573459802538622 1103 44-45 0.2204383812218822 1103 46-47 0.3074822069173635 1103 48-49 0.26734018978580565 1103 50-51 0.23132608099459162 1103 52-53 0.09253918956575546 1103 54-55 0.31336701616209695 1103 56-57 0.07484885588372236 1103 58-59 -0.06999756820950864 1103 60-61 -0.1730166985772641 1103 62-63 0.13966622806416495 1103 64-65 0.08193870311600904 1103 66-67 0.2216559804562408 1103 68-69 0.12627999719173033 1103 70-71 0.14754225352035633 1103 72-73 0.21908393612110544 1103 74-75 0.14184084671621378 1103 76-77 0.36463770286784936 1103 78-79 0.3280191882876302 1103 80-81 0.30245889963309835 1103 82-83 0.45282145279658437 1103 84-85 0.1060062622305189 1103 86-87 -0.07481534516321275 1103 88-89 0.28769861654449613 1103 90-91 0.11118247398959369 1103 92-93 0.27344968452912255 1103 94-95 0.38485524245001557 1103 96-97 0.5416510042996521 1103 98-99 0.33126320398987374 1103 100-101 0.8880397690134494 1104 1 0.010947041551929715 1104 2 0.08444440013801824 1104 3 0.03688866053212081 1104 4 0.035965547762849326 1104 5 -0.1327079835790883 1104 6 0.10437074621079034 1104 7 0.12433666453937064 1104 8 0.10490382084937266 1104 9 0.033236774459957985 1104 10-11 0.15749302780689334 1104 12-13 0.17670927987273188 1104 14-15 0.18853690413560997 1104 16-17 0.12804537343842526 1104 18-19 0.12344679579745588 1104 20-21 0.18813216219182038 1104 22-23 0.20931746803038465 1104 24-25 0.14695328188718548 1104 26-27 0.2017261866922766 1104 28-29 0.21635159585694197 1104 30-31 0.1453759990777499 1104 32-33 0.1045312723776064 1104 34-35 0.22870555073383514 1104 36-37 0.22684128311041007 1104 38-39 0.2345495826327948 1104 40-41 0.3707802889851237 1104 42-43 0.20945363770392333 1104 44-45 0.2604555088099616 1104 46-47 0.026516511869886017 1104 48-49 0.05743871301212522 1104 50-51 0.34157039235014963 1104 52-53 0.2477704572561521 1104 54-55 0.2635209834499861 1104 56-57 0.2968946863228652 1104 58-59 0.0809422997021656 1104 60-61 0.19290618881280608 1104 62-63 0.21566831640813433 1104 64-65 0.1367565443158938 1104 66-67 -0.0715167412012292 1104 68-69 0.028533055608797042 1104 70-71 0.15861206032025166 1104 72-73 0.15180238074605157 1104 74-75 0.037178839417194354 1104 76-77 0.28590447693075305 1104 78-79 0.4190091606165076 1104 80-81 0.2956497783439822 1104 82-83 0.28950424398255237 1104 84-85 0.40221760354465985 1104 86-87 0.35228402878610865 1104 88-89 0.3474059090054169 1104 90-91 0.32089253808794993 1104 92-93 0.33851313805724814 1104 94-95 0.30860625211794 1104 96-97 0.5298009532788974 1104 98-99 0.5253227930635127 1104 100-101 1.1745083522202648 1105 1 -8.073259086245343E-4 1105 2 0.10904399704072887 1105 3 0.11475224488791014 1105 4 0.07975044330837022 1105 5 0.027079163624797786 1105 6 0.05598783588503409 1105 7 0.09263230590660498 1105 8 0.08516512328011316 1105 9 0.10605017801812266 1105 10-11 0.0583809653841314 1105 12-13 0.11146100129077041 1105 14-15 0.16980251039806404 1105 16-17 -0.11084021617734408 1105 18-19 -0.0033219712705516713 1105 20-21 0.0391695988782601 1105 22-23 -0.010709508304266535 1105 24-25 -0.04092158368973031 1105 26-27 0.05258282575969275 1105 28-29 -0.11728381204404315 1105 30-31 0.023544829889239338 1105 32-33 0.053831011212757574 1105 34-35 0.009621828939756938 1105 36-37 0.23396593601795956 1105 38-39 0.05247743784794778 1105 40-41 0.1753330183756887 1105 42-43 0.2869853202797117 1105 44-45 0.18828028530650442 1105 46-47 0.1314666062240022 1105 48-49 0.06987585803772589 1105 50-51 0.11077294529967219 1105 52-53 0.3640823692626256 1105 54-55 0.38512687215569485 1105 56-57 0.25106891010836563 1105 58-59 0.29175295403592116 1105 60-61 0.1967052890666281 1105 62-63 0.19359862505958603 1105 64-65 0.24349810575613162 1105 66-67 0.06155219806590395 1105 68-69 -0.032194823911432024 1105 70-71 -0.20172326939177765 1105 72-73 -0.08619074275351224 1105 74-75 -0.0824080087884731 1105 76-77 -0.10553652709792516 1105 78-79 0.21623310890632297 1105 80-81 0.09851427987221584 1105 82-83 0.05791390134985264 1105 84-85 -0.21211120965712027 1105 86-87 -0.06866396065723634 1105 88-89 0.21081519950373462 1105 90-91 0.22011064857512963 1105 92-93 0.1990130429006065 1105 94-95 0.28065616693554674 1105 96-97 0.2959289722093388 1105 98-99 0.2537319803799356 1105 100-101 0.5830539918677964 1106 1 0.003272555994030313 1106 2 0.06927633009869538 1106 3 0.08056756137021637 1106 4 0.09138958162792932 1106 5 -0.020771523402579817 1106 6 0.0619885104476694 1106 7 0.07692428398820539 1106 8 0.15818652842340697 1106 9 0.22306284317598823 1106 10-11 0.21213212020603578 1106 12-13 0.2504708850998014 1106 14-15 0.2910905828597805 1106 16-17 0.18916418314417882 1106 18-19 0.11567588772630444 1106 20-21 0.05786671536740329 1106 22-23 0.11348785992382915 1106 24-25 0.08364984907152717 1106 26-27 0.2220881953760241 1106 28-29 0.2152849762361626 1106 30-31 0.14471497609336126 1106 32-33 0.1583708666217163 1106 34-35 0.0452028082287228 1106 36-37 0.16629080636017335 1106 38-39 0.23300066382154228 1106 40-41 0.409133494480038 1106 42-43 0.4174574652422436 1106 44-45 0.31181922174607735 1106 46-47 0.2237125576261647 1106 48-49 0.2888844709316487 1106 50-51 0.39976156572132027 1106 52-53 0.4660136714129166 1106 54-55 0.5840366958406662 1106 56-57 0.520935879513523 1106 58-59 0.366760775051965 1106 60-61 0.3807014127755508 1106 62-63 0.4224765047821393 1106 64-65 0.3404919027127704 1106 66-67 0.40309315819339275 1106 68-69 0.34078417666048466 1106 70-71 0.29652910013167855 1106 72-73 0.30934357928660106 1106 74-75 0.21301095326493424 1106 76-77 0.42473728178576664 1106 78-79 0.4499003833311157 1106 80-81 0.46312112717176745 1106 82-83 0.48826469836027186 1106 84-85 0.46010059812185844 1106 86-87 0.5071468587408354 1106 88-89 0.6581206150685368 1106 90-91 0.5185298257064801 1106 92-93 0.578138262255667 1106 94-95 0.583948203674769 1106 96-97 0.42911500974043904 1106 98-99 0.3943477387496479 1106 100-101 0.8625118488116357 1107 1 0.2035666054852996 1107 2 0.26680755316147753 1107 3 0.16137588777384337 1107 4 0.13445856834435688 1107 5 -0.019942132145338576 1107 6 0.14235674060604708 1107 7 0.12394136868743999 1107 8 0.09146223269285514 1107 9 0.18071729862072772 1107 10-11 0.16902822462077438 1107 12-13 0.2851749190165549 1107 14-15 0.2804331225356762 1107 16-17 0.28166833892955623 1107 18-19 0.24580080171057972 1107 20-21 0.338537009115349 1107 22-23 0.26903804407151455 1107 24-25 0.31977887116408965 1107 26-27 0.40137722532512043 1107 28-29 0.3675866660155762 1107 30-31 0.3149444754186774 1107 32-33 0.1929898695972625 1107 34-35 0.26755260704769057 1107 36-37 0.29630325130400337 1107 38-39 0.2946753305505112 1107 40-41 0.394444263553666 1107 42-43 0.3266027874674009 1107 44-45 0.3651208536609971 1107 46-47 0.26973528289227744 1107 48-49 0.3418234309407566 1107 50-51 0.06270720052347656 1107 52-53 0.19674798286467876 1107 54-55 0.3395954917482058 1107 56-57 0.1159671249886145 1107 58-59 0.04178080350609292 1107 60-61 0.08922729953552988 1107 62-63 0.24529925764935712 1107 64-65 0.35805805909419064 1107 66-67 0.5147318565625625 1107 68-69 0.10354656027607945 1107 70-71 0.11200964855679274 1107 72-73 0.38576534144826624 1107 74-75 0.3967160909539338 1107 76-77 0.16712361674301945 1107 78-79 0.40748549645078924 1107 80-81 0.35899625719711636 1107 82-83 0.5796117677675241 1107 84-85 0.36049961871754377 1107 86-87 0.6949930440164671 1107 88-89 0.57700143673366 1107 90-91 0.5808292525218697 1107 92-93 0.7246210375134545 1107 94-95 0.9743508591394487 1107 96-97 0.7574537713802378 1107 98-99 0.7947927385363904 1107 100-101 1.3453110130634336 1108 1 0.09531399775310945 1108 2 0.10165364832098334 1108 3 0.06821364768525484 1108 4 0.03193651035527978 1108 5 -0.09341750946453686 1108 6 -0.005588445509459916 1108 7 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-0.8579151205994755 2316 88-89 -1.6130823828263274 2316 90-91 -1.6829892513711897 2316 92-93 -1.472017510015693 2316 94-95 -1.7341714987893901 2316 96-97 -1.5566013526248916 2316 98-99 -1.8286585717298856 2316 100-101 -2.84732137325253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 22.0 5 56.0 6 173.0 7 352.0 8 856.0 9 1570.0 10 2801.0 11 4557.0 12 6689.0 13 9719.0 14 12486.0 15 16796.0 16 22176.0 17 29637.0 18 26069.0 19 16761.0 20 14768.0 21 15040.0 22 16092.0 23 17778.0 24 21195.0 25 25208.0 26 30475.0 27 38378.0 28 47905.0 29 59435.0 30 73537.0 31 91218.0 32 116055.0 33 150362.0 34 202522.0 35 281771.0 36 417005.0 37 568351.0 38 332356.0 39 15920.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.986641008187576 19.667090170588175 14.965824276158319 22.38044454506593 2 15.678938023708763 21.159704856011384 39.56018795157578 23.601169168704075 3 19.18883521314268 25.409554713025038 30.289774571793625 25.111835502038655 4 13.001307844573114 17.458961862537457 37.08891593347825 32.450814359411176 5 13.524046517844413 37.91144007329796 33.84978460720552 14.714728801652107 6 31.271111005216113 36.85690661438763 17.351705949780705 14.52027643061555 7 27.869368779075653 33.50207885347359 20.786152095829785 17.84240027162097 8 26.48364784767799 34.85858116175167 19.721871755285267 18.935899235285074 9 26.695620707524974 16.494161042643302 20.546398937342524 36.263819312489204 10-11 24.79620073571376 27.116722056781974 27.13691863181513 20.95015857568913 12-13 25.98939544189785 24.48693101675943 27.948537568405317 21.5751359729374 14-15 22.82607967237528 25.46908041697692 26.63979895755771 25.065040953090094 16-17 21.83281405488033 28.937057048157943 27.11467447774635 22.115454419215375 18-19 21.770343656543748 26.921878437859142 30.162563852271134 21.14521405332598 20-21 22.562369884351575 26.069926643812973 30.323126111288158 21.044577360547297 22-23 22.269253264364675 26.114228657609257 30.211265534422093 21.405252543603975 24-25 22.11979104909356 26.274461479353505 30.24568652040302 21.36006095114992 26-27 21.975045512250148 26.525898238703842 30.04746676395802 21.45158948508799 28-29 21.686629022215314 26.492820904676766 30.302362442167496 21.518187630940425 30-31 21.844936066194148 26.352793838349562 30.254046901109543 21.548223194346743 32-33 21.712333012731676 26.501514931244998 30.1106196708196 21.675532385203727 34-35 22.136749241185825 26.464959035389242 29.931491728339125 21.46679999508581 36-37 22.191694123340966 26.26968969304369 29.81510024012956 21.72351594348579 38-39 21.949257107795138 26.588712565910132 29.921749283468124 21.5402810428266 40-41 22.195661363822932 26.368404897183606 29.854607532292942 21.58132620670052 42-43 22.022401259223876 26.56331728651848 29.562853044508042 21.851428409749605 44-45 22.15955299592098 26.77893889582904 29.261384842225258 21.800123266024723 46-47 22.32614450342943 27.02487278434795 28.724854002390465 21.924128709832157 48-49 22.542232295495022 27.16429345786599 28.349377684192973 21.94409656244602 50-51 22.454759269893778 27.265954902137807 28.222900004560604 22.056385823407815 52-53 22.64679137740557 27.32991261437428 28.06818107783361 21.95511493038654 54-55 22.423149705204462 27.275763104785046 28.129547719574 22.171539470436496 56-57 22.385572036970473 27.65434658822798 27.99580952161125 21.964271853190297 58-59 22.59431952438028 27.27645014855205 28.006599169687004 22.122631157380667 60-61 22.575538711262677 27.244456523357808 28.36824842519099 21.81175634018853 62-63 22.43954895184429 27.25208564539133 28.405617216640188 21.902748186124192 64-65 22.316928046675926 27.239240004683378 28.269564503570333 22.174267445070363 66-67 22.311389118569345 27.592579424660073 27.94712704185512 22.14890441491546 68-69 22.326901197872303 27.385432240694303 28.17868385940041 22.108982702032986 70-71 22.342843825551377 27.388698649261418 28.09712719548071 22.171330329706496 72-73 22.502586474238196 27.34987559980328 27.93719791116378 22.210340014794745 74-75 22.388441725662243 27.6389688558088 27.704772866335208 22.26781655219375 76-77 22.484337401876665 27.5365572309111 27.781987728288986 22.197117638923245 78-79 22.438188558286807 27.5486417537743 27.686313855535506 22.32685583240339 80-81 22.30274363513506 27.85983801402439 27.549101184459623 22.28831716638093 82-83 22.47001688324501 27.754993773489023 27.58822751987551 22.186761823390462 84-85 22.426969220316266 27.666179577628654 27.411323424018764 22.49552777803632 86-87 22.382480094189038 27.535476785473296 27.59921260941797 22.482830510919694 88-89 22.48180905544094 27.62313243700113 27.4977424781382 22.39731602941973 90-91 22.345840017542443 27.7124238242152 27.577337338708745 22.364398819533612 92-93 22.369540363180953 27.470078355265386 27.60825271146 22.552128570093657 94-95 22.27898152674194 27.423449396317856 27.581373583028775 22.71619549391143 96-97 22.38944161257321 27.392602338266897 27.58984055646642 22.628115492693475 98-99 22.106055546310373 27.413393405937196 27.42039244505847 23.06015860269396 100-101 21.92688129449062 27.730596406331355 27.431437368800303 22.91108493037772 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 746.0 1 591.0 2 477.0 3 562.0 4 527.0 5 493.5 6 876.0 7 1899.5 8 3109.5 9 3707.5 10 3480.0 11 3200.0 12 3329.5 13 3639.0 14 4049.5 15 4400.5 16 4653.5 17 4890.0 18 5157.0 19 5512.0 20 6099.0 21 6919.5 22 8126.5 23 9783.0 24 11493.5 25 13814.0 26 17325.0 27 21358.0 28 24867.5 29 28457.0 30 33165.5 31 38127.0 32 43097.5 33 48392.5 34 55175.0 35 59523.5 36 64689.0 37 71990.0 38 75835.0 39 76722.0 40 77349.5 41 81070.5 42 84734.5 43 87818.0 44 91304.0 45 93952.5 46 94539.0 47 96453.0 48 103006.0 49 103787.0 50 99136.5 51 95280.0 52 90832.5 53 89503.5 54 87417.0 55 80796.5 56 72889.0 57 65391.0 58 59805.0 59 54389.0 60 47446.0 61 38890.0 62 30984.0 63 24326.0 64 18114.0 65 13556.5 66 9814.0 67 7649.5 68 5606.0 69 3284.0 70 2085.5 71 1409.0 72 1028.5 73 834.5 74 620.5 75 439.0 76 298.0 77 155.0 78 83.0 79 53.5 80 29.0 81 12.5 82 12.5 83 11.0 84 4.5 85 1.5 86 1.0 87 3.0 88 3.0 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02110867842175586 2 0.0032761264569920916 3 4.0951580712401145E-4 4 0.0 5 0.0028293819401295334 6 0.0 7 1.1168612921563949E-4 8 0.0 9 1.1168612921563949E-4 10-11 0.0 12-13 0.006645324688330549 14-15 0.003685642264116103 16-17 0.0 18-19 5.956593558167439E-4 20-21 0.0010051751629407554 22-23 0.0017869780674502319 24-25 5.025875814703777E-4 26-27 3.350583876469184E-4 28-29 5.5843064607819746E-5 30-31 1.8614354869273247E-5 32-33 1.3030048408491272E-4 34-35 0.0 36-37 0.001824206777188778 38-39 0.009121033885943892 40-41 9.12103388594389E-4 42-43 0.0 44-45 0.0014333053249340401 46-47 3.9090145225473814E-4 48-49 1.1168612921563949E-4 50-51 0.0019172785515351443 52-53 6.142737106860171E-4 54-55 0.0 56-57 3.536727425161917E-4 58-59 1.3030048408491272E-4 60-61 0.002196493874574243 62-63 0.0 64-65 1.3030048408491272E-4 66-67 0.0 68-69 1.1168612921563949E-4 70-71 4.8397322660110437E-4 72-73 3.7228709738546494E-4 74-75 0.003443655650815551 76-77 9.307177434636623E-5 78-79 2.4198661330055219E-4 80-81 0.0023826374232669757 82-83 1.4891483895418598E-4 84-85 0.0027549245206524406 86-87 4.2813016199328465E-4 88-89 0.0029224537144758997 90-91 0.0015636058090189526 92-93 0.0 94-95 0.0 96-97 2.6060096816982544E-4 98-99 5.956593558167439E-4 100-101 0.0025873953268289816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2686099.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.164443753461875 #Duplication Level Percentage of deduplicated Percentage of total 1 72.10080592840899 35.447960176465344 2 14.817242406327304 14.569629617345775 3 5.2955607115934376 7.810598902445342 4 2.556389263269577 5.0273382458388385 5 1.392104280892067 3.4221016308435757 6 0.8774537049495413 2.588371399395507 7 0.5565413207773411 1.915343112328448 8 0.3982745050786579 1.566475560270203 9 0.3127283030757737 1.3837601760016007 >10 1.5003795365040369 13.371226456713337 >50 0.12228846439939856 4.139212756393961 >100 0.06589356320688146 5.986517468788992 >500 0.0033486404693386677 1.0545558983198855 >1k 7.610546521158047E-4 0.5331523912522887 >5k 7.610546521158046E-5 0.2744524380542872 >10k+ 1.5221093042316091E-4 0.9093037695426431 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13448 0.5006516885639732 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10854 0.40408041550218365 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7335 0.27307258593223854 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2749 0.10234172307126432 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.7228709738546495E-5 2 0.0 0.0 0.0 0.0 3.7228709738546495E-5 3 0.0 0.0 0.0 0.0 3.7228709738546495E-5 4 0.0 0.0 0.0 0.0 3.7228709738546495E-5 5 0.0 0.0 0.0 0.0 3.7228709738546495E-5 6 0.0 0.0 0.0 0.0 3.7228709738546495E-5 7 0.0 0.0 0.0 0.0 3.7228709738546495E-5 8 0.0 0.0 0.0 0.0 3.7228709738546495E-5 9 0.0 0.0 0.0 0.0 3.7228709738546495E-5 10-11 0.0 0.0 0.0 1.8614354869273247E-5 3.7228709738546495E-5 12-13 0.0 0.0 0.0 3.7228709738546495E-5 1.1168612921563948E-4 14-15 0.0 0.0 0.0 5.5843064607819746E-5 1.1168612921563948E-4 16-17 0.0 0.0 0.0 1.1168612921563948E-4 1.1168612921563948E-4 18-19 0.0 0.0 0.0 1.675291938234592E-4 1.1168612921563948E-4 20-21 0.0 0.0 0.0 2.9782967790837196E-4 1.1168612921563948E-4 22-23 0.0 0.0 0.0 4.839732266011044E-4 1.1168612921563948E-4 24-25 0.0 0.0 0.0 7.073454850323835E-4 1.1168612921563948E-4 26-27 0.0 0.0 0.0 0.0010796325824178484 1.4891483895418598E-4 28-29 0.0 0.0 0.0 0.0020103503258815107 1.4891483895418598E-4 30-31 0.0 0.0 0.0 0.0036670279092468295 1.4891483895418598E-4 32-33 0.0 0.0 0.0 0.006496409849376363 1.4891483895418598E-4 34-35 0.0 0.0 0.0 0.00887904727264334 1.8614354869273247E-4 36-37 0.0 0.0 0.0 0.01129891340564886 1.8614354869273247E-4 38-39 0.0 0.0 0.0 0.014817026475941504 1.8614354869273247E-4 40-41 0.0 0.0 0.0 0.019303085999436358 1.8614354869273247E-4 42-43 0.0 0.0 0.0 0.025203836492995978 1.8614354869273247E-4 44-45 0.0 0.0 0.0 0.030806757308647223 2.0475790356200573E-4 46-47 1.8614354869273247E-5 0.0 0.0 0.037452081996977776 2.2337225843127896E-4 48-49 3.7228709738546495E-5 0.0 0.0 0.04316668894184466 2.2337225843127896E-4 50-51 3.7228709738546495E-5 0.0 0.0 0.05158037734275617 3.350583876469185E-4 52-53 5.5843064607819746E-5 0.0 0.0 0.07529506544621029 3.350583876469185E-4 54-55 7.445741947709299E-5 0.0 0.0 0.11133245647312329 3.350583876469185E-4 56-57 7.445741947709299E-5 0.0 0.0 0.13562418957752487 3.350583876469185E-4 58-59 7.445741947709299E-5 0.0 0.0 0.17356024480110377 3.350583876469185E-4 60-61 7.445741947709299E-5 0.0 0.0 0.2195004726184701 3.350583876469185E-4 62-63 7.445741947709299E-5 0.0 0.0 0.24872500976322914 3.7228709738546494E-4 64-65 7.445741947709299E-5 0.0 0.0 0.2699639886690699 3.909014522547382E-4 66-67 7.445741947709299E-5 0.0 0.0 0.3392652318473742 4.0951580712401145E-4 68-69 7.445741947709299E-5 0.0 0.0 0.41480228390688506 4.0951580712401145E-4 70-71 7.445741947709299E-5 0.0 0.0 0.4432822468568731 4.0951580712401145E-4 72-73 7.445741947709299E-5 0.0 0.0 0.5050260619582525 5.025875814703777E-4 74-75 7.445741947709299E-5 0.0 0.0 0.5940585212979864 5.956593558167439E-4 76-77 7.445741947709299E-5 0.0 0.0 0.6763525841750435 5.956593558167439E-4 78-79 7.445741947709299E-5 0.0 0.0 0.7582557455998458 5.956593558167439E-4 80-81 7.445741947709299E-5 0.0 0.0 0.879472424508553 5.956593558167439E-4 82-83 7.445741947709299E-5 0.0 0.0 1.013458550857582 5.956593558167439E-4 84-85 7.445741947709299E-5 0.0 0.0 1.1371137102541642 5.956593558167439E-4 86-87 7.445741947709299E-5 0.0 0.0 1.3548830478697917 5.956593558167439E-4 88-89 7.445741947709299E-5 0.0 0.0 1.6253682384752013 5.956593558167439E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8250 0.0 42.959446 1 GTATCAA 14130 0.0 37.52285 1 ATCAACG 18115 0.0 28.974022 3 TCAACGC 18335 0.0 28.652271 4 TATCAAC 18555 0.0 28.364279 2 CAACGCA 18625 0.0 28.257147 5 AACGCAG 19125 0.0 27.496122 6 ACGCAGA 21650 0.0 24.13571 7 CGCAGAG 21685 0.0 23.989008 8 GTACATG 12510 0.0 22.899912 1 TACATGG 12690 0.0 22.196577 2 GCAGAGT 23020 0.0 22.16443 9 GAGTACT 12975 0.0 21.619715 12-13 AGTACTT 13520 0.0 21.573921 12-13 ACATGGG 12785 0.0 21.399645 3 AGAGTAC 19990 0.0 20.87789 10-11 GTACTTT 14040 0.0 19.910233 14-15 CATGGGG 5420 0.0 19.192417 4 GTATAGG 870 0.0 19.112722 1 TACTTTT 15290 0.0 18.950441 14-15 >>END_MODULE