##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936951_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 111775 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01422500559159 33.0 31.0 34.0 30.0 34.0 2 32.15015880116305 33.0 31.0 34.0 31.0 34.0 3 32.1077432341758 34.0 31.0 34.0 30.0 34.0 4 35.703180496533214 37.0 35.0 37.0 35.0 37.0 5 35.72518899575039 37.0 35.0 37.0 35.0 37.0 6 35.82780585998658 37.0 35.0 37.0 35.0 37.0 7 35.78141802728696 37.0 35.0 37.0 35.0 37.0 8 35.780917020800715 37.0 35.0 37.0 35.0 37.0 9 37.42915678819056 39.0 37.0 39.0 35.0 39.0 10-11 37.34861552225453 39.0 37.0 39.0 35.0 39.0 12-13 37.289662267949005 39.0 37.0 39.0 34.5 39.0 14-15 38.419991053455604 40.0 38.0 41.0 34.0 41.0 16-17 38.32438828002684 40.0 38.0 41.0 34.0 41.0 18-19 38.32218295683292 40.0 38.0 41.0 34.0 41.0 20-21 38.29254752851711 40.0 38.0 41.0 34.0 41.0 22-23 38.1900022366361 40.0 38.0 41.0 34.0 41.0 24-25 38.02948333706106 40.0 37.5 41.0 34.0 41.0 26-27 37.910794005815255 40.0 37.0 41.0 34.0 41.0 28-29 37.93765153209573 40.0 37.0 41.0 34.0 41.0 30-31 37.75362558711697 40.0 37.0 41.0 34.0 41.0 32-33 37.48560948333706 40.0 35.5 41.0 33.0 41.0 34-35 37.2761350928204 40.0 35.0 41.0 33.0 41.0 36-37 37.07102214269738 39.0 35.0 41.0 33.0 41.0 38-39 36.86059047193022 39.0 35.0 41.0 33.0 41.0 40-41 36.66091702080072 39.0 35.0 41.0 31.5 41.0 42-43 36.40415119660031 39.0 35.0 41.0 31.0 41.0 44-45 36.07110713486916 39.0 35.0 41.0 30.5 41.0 46-47 35.62169984343547 39.0 35.0 41.0 29.5 41.0 48-49 35.18300156564527 39.0 35.0 40.5 27.0 41.0 50-51 34.4796734511295 38.5 34.5 39.5 24.5 40.5 52-53 34.67383583091031 38.5 35.0 40.0 24.5 40.5 54-55 34.96569447550884 39.0 35.0 41.0 24.0 41.0 56-57 34.86188772086781 39.0 35.0 41.0 23.5 41.0 58-59 34.66908968910758 39.0 35.0 41.0 22.5 41.0 60-61 34.395777231044505 39.0 35.0 41.0 21.5 41.0 62-63 34.07067322746589 38.0 35.0 40.0 19.0 41.0 64-65 33.752498322522925 38.0 34.5 40.0 15.0 41.0 66-67 33.366692015209125 37.0 34.0 40.0 9.5 41.0 68-69 32.916103779915005 37.0 34.0 39.5 4.5 41.0 70-71 32.50914336837397 36.0 33.0 39.0 2.0 41.0 72-73 32.05253410870051 36.0 33.0 39.0 2.0 40.5 74-75 31.56754641019906 35.0 32.5 38.0 2.0 40.0 76-77 31.08187430105122 35.0 32.0 37.0 2.0 39.5 78-79 30.621851934690227 35.0 31.5 37.0 2.0 39.0 80-81 30.167389845672112 35.0 31.0 36.0 2.0 39.0 82-83 29.69964661149631 35.0 31.0 36.0 2.0 37.5 84-85 29.261735629613064 35.0 30.0 35.5 2.0 37.0 86-87 28.875097293670322 34.5 30.0 35.0 2.0 36.5 88-89 28.4067770073809 34.0 29.0 35.0 2.0 36.0 90-91 27.993504808767614 34.0 29.0 35.0 2.0 36.0 92-93 27.639709237307088 34.0 29.0 35.0 2.0 36.0 94-95 27.275401476179823 34.0 27.0 35.0 2.0 35.0 96-97 26.838210691120555 34.0 26.0 35.0 2.0 35.0 98-99 26.30777902035339 34.0 25.0 35.0 2.0 35.0 100-101 24.394945202415567 31.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06579141855672077 1101 2 0.09740396423076447 1101 3 -0.08854858251338982 1101 4 -0.020212757915821555 1101 5 -0.11215932409507445 1101 6 0.21207727260319587 1101 7 0.22539487386146817 1101 8 -0.0354595607415078 1101 9 0.12810382000811416 1101 10-11 -0.12846010881366965 1101 12-13 0.26503468540765596 1101 14-15 0.31622381658088017 1101 16-17 0.3014114846334053 1101 18-19 0.06939077829639473 1101 20-21 0.21653010811418483 1101 22-23 -0.08760602644210991 1101 24-25 0.08431384961330224 1101 26-27 -0.07960584044656116 1101 28-29 -0.1407462258039729 1101 30-31 0.09570878634769286 1101 32-33 0.18240759323884248 1101 34-35 -0.1854635290904838 1101 36-37 -0.3879497061453563 1101 38-39 0.07024256669600248 1101 40-41 0.2205950072458407 1101 42-43 0.4698057217821159 1101 44-45 0.5620682599239402 1101 46-47 0.30306328090853185 1101 48-49 0.4630204206980508 1101 50-51 0.11362791257734273 1101 52-53 0.9006327021853267 1101 54-55 0.5520473132864154 1101 56-57 0.035122600095704115 1101 58-59 -0.25681195461789486 1101 60-61 0.4330751666625119 1101 62-63 0.39405133907981593 1101 64-65 0.30070454266854796 1101 66-67 0.45658396868703477 1101 68-69 0.724070948348583 1101 70-71 0.9131534553514413 1101 72-73 0.7575900546971681 1101 74-75 0.5944372598074601 1101 76-77 1.136931543676841 1101 78-79 1.2207488207959294 1101 80-81 1.5155584980085415 1101 82-83 1.2041772425019985 1101 84-85 1.312133946923364 1101 86-87 1.695106288910079 1101 88-89 1.4033759432929571 1101 90-91 0.9091293882662583 1101 92-93 0.0411483589667796 1101 94-95 0.5213787284505891 1101 96-97 0.5874909445813685 1101 98-99 0.4142672538223806 1101 100-101 0.7530524574078363 1102 1 0.7331104345661323 1102 2 0.28683430053824566 1102 3 0.5181775671091202 1102 4 0.4561656451955898 1102 5 0.5138415910273224 1102 6 0.4664241039918977 1102 7 0.6709450912030377 1102 8 0.474721175932018 1102 9 0.5887535569104259 1102 10-11 0.8399967660433418 1102 12-13 0.5624208670594584 1102 14-15 0.6908234505319157 1102 16-17 0.7699255546402668 1102 18-19 0.5108229448486767 1102 20-21 0.8879153746435762 1102 22-23 1.1193260257198645 1102 24-25 0.8013980408738774 1102 26-27 0.8815014576545508 1102 28-29 0.8709787799155464 1102 30-31 0.7326625726666123 1102 32-33 0.45617217800205623 1102 34-35 0.08689977450137576 1102 36-37 -0.03136654439747133 1102 38-39 -0.058675301068809915 1102 40-41 0.21001390452335755 1102 42-43 0.19055040263317835 1102 44-45 0.3441833365654432 1102 46-47 -0.10988570101780226 1102 48-49 -0.1878317870347317 1102 50-51 -0.2918571022933918 1102 52-53 -0.2831925094367236 1102 54-55 0.1999539708018645 1102 56-57 0.043187116224736144 1102 58-59 0.14083732472321486 1102 60-61 0.3011545535305018 1102 62-63 0.10458668568242047 1102 64-65 -0.040407331044555406 1102 66-67 -0.1443368732255763 1102 68-69 -0.18787711845535426 1102 70-71 -0.4072537741155031 1102 72-73 -0.540654054202399 1102 74-75 -0.49078351347096216 1102 76-77 -0.6125822975494266 1102 78-79 -0.6730969810800715 1102 80-81 -0.8208061782669098 1102 82-83 -0.3984594539061419 1102 84-85 0.08401109174148402 1102 86-87 0.14400242251794992 1102 88-89 0.09872597761004798 1102 90-91 0.2393169883577677 1102 92-93 0.04380793343486289 1102 94-95 -0.029553552491986324 1102 96-97 0.21137563915926805 1102 98-99 -0.5592227942215438 1102 100-101 -0.4756995444224117 1103 1 0.1206379474054593 1103 2 0.4348900598926093 1103 3 0.48164051648549844 1103 4 0.39096632995958913 1103 5 -0.0640503140839499 1103 6 0.13504723923278306 1103 7 0.17033369477358917 1103 8 0.18589750266227156 1103 9 0.14756989342301807 1103 10-11 0.2567734840673097 1103 12-13 0.3265196687224545 1103 14-15 0.4081871091281499 1103 16-17 0.682251306657875 1103 18-19 0.6272328250149783 1103 20-21 0.20921642624543324 1103 22-23 0.039868945749212514 1103 24-25 0.45948070167113997 1103 26-27 0.36677903163352 1103 28-29 0.47232385206032035 1103 30-31 0.35413481526871493 1103 32-33 0.5826578713256012 1103 34-35 0.5189858591186365 1103 36-37 0.35012037171893695 1103 38-39 0.25311242209088647 1103 40-41 0.24033916742381933 1103 42-43 0.5879091700579764 1103 44-45 0.7277245446847829 1103 46-47 0.7709164510976336 1103 48-49 0.9791772616324224 1103 50-51 0.8563698480612203 1103 52-53 1.001522579827153 1103 54-55 1.193204154220787 1103 56-57 0.9845664265695646 1103 58-59 0.33342719999834713 1103 60-61 0.2742041989205788 1103 62-63 -0.18631573544743674 1103 64-65 0.24998151708457073 1103 66-67 0.5507719553388668 1103 68-69 0.8357227837212164 1103 70-71 0.5994715866083951 1103 72-73 0.8102874962989475 1103 74-75 1.0066730773825476 1103 76-77 0.8530327672585933 1103 78-79 1.0624618352564426 1103 80-81 0.7656697327137678 1103 82-83 0.5543162858835373 1103 84-85 1.0504821115507248 1103 86-87 0.8416302043717039 1103 88-89 1.103348134616649 1103 90-91 1.257683337098296 1103 92-93 1.2195966348288465 1103 94-95 1.513342020997868 1103 96-97 1.998169476283259 1103 98-99 1.3777544618312803 1103 100-101 1.5811114118016043 1104 1 0.6798377368237247 1104 2 0.5374433633935283 1104 3 0.3330224591019757 1104 4 0.46482787247077795 1104 5 0.6053363666215006 1104 6 0.6314936725046962 1104 7 0.6783621233074157 1104 8 0.6441759970028897 1104 9 0.634013694423281 1104 10-11 0.7668550844884692 1104 12-13 0.9174909769614672 1104 14-15 0.9939201967827955 1104 16-17 0.350321851538105 1104 18-19 0.371902474922841 1104 20-21 0.7430937623865219 1104 22-23 0.5281290141882735 1104 24-25 0.5738792275990221 1104 26-27 0.8955234380562658 1104 28-29 0.7317605459271306 1104 30-31 0.3831687715730041 1104 32-33 0.4038739808281022 1104 34-35 0.19658522737344697 1104 36-37 0.17062576787582628 1104 38-39 0.18351515965340326 1104 40-41 0.1260985777949699 1104 42-43 -0.24107214081330142 1104 44-45 -0.31828756376480527 1104 46-47 -0.1426397479021091 1104 48-49 -0.8312728568198011 1104 50-51 -0.2535538160609221 1104 52-53 0.024370697752921444 1104 54-55 0.24153266172276489 1104 56-57 0.020286352865959145 1104 58-59 -0.1306422919732242 1104 60-61 -0.03266603008705715 1104 62-63 0.11376323270206967 1104 64-65 -0.2599947924705788 1104 66-67 -0.413244891199696 1104 68-69 -0.07951806913479942 1104 70-71 -0.2636502875690283 1104 72-73 -0.6110075813530642 1104 74-75 -0.5677149194587265 1104 76-77 -0.2846087173426177 1104 78-79 -0.32492958353797974 1104 80-81 -0.1364360846065793 1104 82-83 -0.3574762911104088 1104 84-85 -0.30984955467723196 1104 86-87 -0.09943323337507337 1104 88-89 -0.2633009835723463 1104 90-91 -0.20654409117230443 1104 92-93 0.15660637423395585 1104 94-95 0.06167052849984245 1104 96-97 0.060896509713106894 1104 98-99 0.3575386155559457 1104 100-101 0.6705296797668581 1105 1 -0.1940172250083343 1105 2 0.1871889104673201 1105 3 0.21037614866940402 1105 4 0.05935713455729541 1105 5 0.22359336407696873 1105 6 0.13734608980749385 1105 7 0.24878197063566176 1105 8 -0.39513698009634624 1105 9 -0.12915424450801538 1105 10-11 -0.06434720558786466 1105 12-13 -0.15149757265685082 1105 14-15 0.020256074645395472 1105 16-17 -0.04576593472143742 1105 18-19 -0.04431889912296327 1105 20-21 -0.19825483812238076 1105 22-23 -0.06515978988296922 1105 24-25 0.2610611614412619 1105 26-27 0.1353135143921449 1105 28-29 0.14057097849710232 1105 30-31 0.22769803365952157 1105 32-33 0.28711189431411555 1105 34-35 0.3000203418772571 1105 36-37 0.4767008314890546 1105 38-39 0.906129663470189 1105 40-41 1.0509713513318673 1105 42-43 1.0989723884487788 1105 44-45 1.2183854642250154 1105 46-47 1.065025646499933 1105 48-49 1.5037462271496693 1105 50-51 1.2813698480612175 1105 52-53 1.6918961430455468 1105 54-55 1.9302731197380325 1105 56-57 2.1431871162247305 1105 58-59 2.1888869701132805 1105 60-61 2.128370865587243 1105 62-63 1.6593739197249704 1105 64-65 1.3611884136362917 1105 66-67 1.5241914955687506 1105 68-69 1.5134526687786902 1105 70-71 1.7573738854589678 1105 72-73 1.5559771514713603 1105 74-75 1.6586845716354226 1105 76-77 1.161797135074682 1105 78-79 1.5575767777851723 1105 80-81 1.3139455947827337 1105 82-83 1.4896611134697437 1105 84-85 1.3435855598265896 1105 86-87 0.9121761813831952 1105 88-89 0.8287791690994091 1105 90-91 0.7925971302017452 1105 92-93 0.7411483589667789 1105 94-95 1.2920776531817744 1105 96-97 1.6993189015706172 1105 98-99 1.1353694043600129 1105 100-101 1.3336976186981566 1106 1 0.2836143539390328 1106 2 0.2828029455550407 1106 3 0.33318316621326716 1106 4 -0.4046779531620075 1106 5 0.020523188638371437 1106 6 0.11980223015837765 1106 7 0.02115039168829469 1106 8 0.2759156514825989 1106 9 0.24847733443935738 1106 10-11 0.3402580575700327 1106 12-13 0.3136340062905134 1106 14-15 0.5018350220138146 1106 16-17 0.4746288021206695 1106 18-19 0.37278636403494403 1106 20-21 0.3219206004741153 1106 22-23 0.04295424520474711 1106 24-25 0.4003155474061657 1106 26-27 0.39781351439214063 1106 28-29 0.6710534346374502 1106 30-31 0.4820839985717953 1106 32-33 0.08864698203340993 1106 34-35 -0.13419018443852337 1106 36-37 -0.21430794044076862 1106 38-39 0.38178755820703714 1106 40-41 0.7356204741388908 1106 42-43 0.5895425638873775 1106 44-45 0.8778152887864152 1106 46-47 0.6034028394823849 1106 48-49 1.099140963991772 1106 50-51 0.745843532271742 1106 52-53 0.6298347395367685 1106 54-55 0.43948364605382295 1106 56-57 0.6589765899089457 1106 58-59 0.5472203034466148 1106 60-61 0.9005199883942581 1106 62-63 0.8817423407776062 1106 64-65 0.7782936767941848 1106 66-67 0.9625686885512081 1106 68-69 1.3005140722874629 1106 70-71 1.6352247626519514 1106 72-73 1.4467666251555684 1106 74-75 1.7643863260213912 1106 76-77 1.869911170162407 1106 78-79 2.1343311637500904 1106 80-81 2.208682436887994 1106 82-83 2.3751874292592134 1106 84-85 1.9383224019318526 1106 86-87 1.561956883137583 1106 88-89 1.3728581164678282 1106 90-91 1.209044498622795 1106 92-93 1.4372009905457261 1106 94-95 1.5438320391466895 1106 96-97 1.5642311822723691 1106 98-99 1.9654132640091362 1106 100-101 1.766592355540265 1107 1 0.37060541650110324 1107 2 0.4227235016622899 1107 3 0.07578495370085392 1107 4 0.18231310940006296 1107 5 0.40959965338514337 1107 6 0.18514483194586262 1107 7 0.47189517818282667 1107 8 0.2642969821678065 1107 9 0.4449023592655692 1107 10-11 0.4198509076196899 1107 12-13 0.7279835594186181 1107 14-15 0.22119947087180236 1107 16-17 0.5091868954672449 1107 18-19 0.6835112895562858 1107 20-21 0.6435690612486837 1107 22-23 0.35928989565162084 1107 24-25 0.18283789100100023 1107 26-27 0.7864927596751627 1107 28-29 0.7145489659184889 1107 30-31 0.4921634424645518 1107 32-33 0.32901441003738086 1107 34-35 0.3537939267829202 1107 36-37 0.17253416482238748 1107 38-39 0.2224032483758549 1107 40-41 0.5876065714576484 1107 42-43 0.28348496706513515 1107 44-45 0.20745779126904296 1107 46-47 0.5597583509024417 1107 48-49 1.2695481139421148 1107 50-51 1.0931623008914073 1107 52-53 1.3207483443034036 1107 54-55 1.3231976480399155 1107 56-57 1.198847493583223 1107 58-59 1.454767473257938 1107 60-61 1.4468457083545374 1107 62-63 1.4768267499136556 1107 64-65 1.844678979674029 1107 66-67 2.1311883509146625 1107 68-69 1.6935627316717756 1107 70-71 1.3813518728803516 1107 72-73 1.2989016797732447 1107 74-75 1.4028669615725313 1107 76-77 1.3390770092885198 1107 78-79 1.4460987903637914 1107 80-81 1.6111154061034902 1107 82-83 1.825981868186723 1107 84-85 1.4181138617133797 1107 86-87 1.4282925335844538 1107 88-89 1.1931659615522392 1107 90-91 0.756512224541364 1107 92-93 1.0859596797214977 1107 94-95 2.298995892175487 1107 96-97 2.341142800941686 1107 98-99 2.5217687754291944 1107 100-101 2.251150448886836 1108 1 0.08315668803514242 1108 2 0.1386381858296417 1108 3 0.16110078635056624 1108 4 0.4557339461514971 1108 5 0.6022165524827656 1108 6 0.3605344956045968 1108 7 0.3868254488965306 1108 8 -0.03209350183547599 1108 9 0.1871501033180749 1108 10-11 0.14597888136866288 1108 12-13 0.043611122995315554 1108 14-15 0.6485169442106056 1108 16-17 0.5036905870176938 1108 18-19 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-1.8029667299291106 2316 58-59 -1.9963694401431198 2316 60-61 -1.430069305352923 2316 62-63 -1.159643174292114 2316 64-65 -1.7766320991842193 2316 66-67 -1.1378811540038996 2316 68-69 -1.1496883568623346 2316 70-71 -2.679485088900009 2316 72-73 -2.4151766946824864 2316 74-75 -3.074435086484236 2316 76-77 -3.386813976036425 2316 78-79 -3.6041753589669625 2316 80-81 -3.1262253453882067 2316 82-83 -3.3122619634533343 2316 84-85 -2.722226405985378 2316 86-87 -2.693058861351844 2316 88-89 -3.2390627112424717 2316 90-91 -3.051099450994837 2316 92-93 -3.0154755726571523 2316 94-95 -3.7032214921173683 2316 96-97 -3.7716212693695574 2316 98-99 -3.3982844417938303 2316 100-101 -3.197285287284746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 382.0 3 79.0 4 70.0 5 75.0 6 85.0 7 84.0 8 111.0 9 144.0 10 182.0 11 245.0 12 359.0 13 497.0 14 650.0 15 854.0 16 1171.0 17 1723.0 18 1551.0 19 829.0 20 764.0 21 695.0 22 748.0 23 776.0 24 930.0 25 1057.0 26 1155.0 27 1289.0 28 1463.0 29 1806.0 30 2087.0 31 2507.0 32 3326.0 33 4295.0 34 6156.0 35 9751.0 36 13286.0 37 21006.0 38 24468.0 39 5119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.3882692066675 24.73546631335828 17.204984512917836 19.67127996705638 2 13.675519816609057 25.095366871429338 40.7659795476118 20.463133764349802 3 15.5626975103623 32.06628291093346 33.56221408556619 18.808805493138053 4 10.012540308133286 21.925833034754568 42.02973844500179 26.031888212110353 5 9.417875694071288 40.8400501522479 37.43059287121619 12.311481282464625 6 21.60651920838184 43.75929076743978 19.978508104235697 14.65568191994269 7 20.550619760693557 38.31142079243391 26.008454538941034 15.129504907931505 8 21.098962111239466 36.07088807099553 23.531150095371142 19.298999722393862 9 23.83311089813065 19.215714375285508 24.90796556882205 32.043209157761794 10-11 19.57228446307192 30.40219231079229 30.465328712286077 19.56019451384971 12-13 22.533199609574382 30.151871983380047 29.819652019736193 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32.01766948924972 34.59872178162875 17.050967614887487 40-41 15.488153695339813 32.07387546272879 35.48564701458798 16.95232382734341 42-43 15.657171503768822 32.44633233666947 33.82495121121515 18.071544948346553 44-45 15.784150058406771 32.63170614904692 33.54159860002775 18.042545192518563 46-47 16.646902199449578 32.31020517754458 32.607790929228294 18.43510169377755 48-49 16.48623610968149 32.73125170622116 32.554028472076155 18.228483712021195 50-51 16.740870670252324 32.55102177794284 32.78325420725513 17.924853344549696 52-53 16.105024792796662 33.39867175053256 32.835687306446125 17.660616150224655 54-55 16.47772790132518 32.45778527665021 32.61532127049199 18.44916555153262 56-57 16.813838476514423 33.405240897800255 31.495513236511734 18.285407389173585 58-59 16.367827410258705 31.94342493957569 32.799659833497444 18.88908781666816 60-61 16.793852130673553 32.72075442985852 32.133090450124655 18.352302989343276 62-63 17.23011230121253 32.530648965835184 32.71058155825205 17.528657174700225 64-65 17.332056405597598 31.93870746868469 32.84404326636922 17.885192859348496 66-67 16.53029434935186 32.41737090883048 32.232507340829336 18.819827400988327 68-69 16.008808246093803 32.418194757124255 33.1244657091578 18.448531287624146 70-71 16.741243220991212 32.345939754076355 32.390685686671084 18.522131338261353 72-73 16.728862399770904 31.47775272050401 32.205756013745706 19.587628865979383 74-75 16.468508781742923 32.365141514710814 32.3423201700414 18.824029533504866 76-77 16.632356231819198 31.416871783396733 33.22935779816514 18.721414186618933 78-79 16.467181121535486 31.250895223089593 32.96211415884838 19.319809496526535 80-81 16.56746475721638 32.473036473484 32.04922801521593 18.910270754083687 82-83 16.69419268210809 31.865279176457424 31.94226250419604 19.498265637238447 84-85 16.245333858507372 31.00645426957541 31.915960218066587 20.83225165385063 86-87 16.73835879485607 31.635699546571534 31.913665071684665 19.712276586887725 88-89 17.552727126209334 30.100686314451103 32.29349008582288 20.05309647351668 90-91 16.645331089367723 29.347757973985516 33.56056469155917 20.446346245087597 92-93 16.71807222443562 30.556836342311772 32.951783228209344 19.773308205043268 94-95 16.198393725321658 29.793530133319006 33.27513497542239 20.73294116593695 96-97 16.2677519296549 29.715342323465677 32.43208152008453 21.584824226794893 98-99 16.215695758096878 29.944110060189168 32.19403840642018 21.646155775293778 100-101 16.349663440653146 29.97147194890926 32.878914769648304 20.799949840789292 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1335.0 1 881.5 2 399.0 3 349.0 4 291.0 5 278.0 6 400.0 7 697.0 8 1002.0 9 1048.5 10 791.0 11 536.5 12 477.5 13 474.5 14 497.5 15 454.5 16 386.0 17 386.5 18 371.0 19 363.0 20 374.5 21 413.0 22 484.0 23 528.5 24 587.0 25 737.5 26 982.0 27 1213.0 28 1329.0 29 1548.0 30 2014.0 31 2411.5 32 3040.0 33 4096.0 34 5036.5 35 5528.0 36 6435.5 37 7360.0 38 7073.0 39 6355.5 40 5520.0 41 4679.5 42 3923.5 43 3677.0 44 3621.0 45 3232.5 46 2734.5 47 2537.5 48 4245.5 49 3920.0 50 1611.0 51 867.0 52 456.5 53 296.0 54 241.0 55 228.0 56 199.0 57 192.0 58 187.0 59 189.0 60 186.5 61 169.0 62 155.0 63 106.0 64 63.0 65 50.5 66 38.5 67 33.0 68 27.5 69 19.0 70 15.5 71 14.0 72 11.0 73 6.5 74 5.5 75 6.0 76 3.5 77 1.5 78 1.5 79 1.5 80 2.0 81 1.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06173115634086334 2 0.09036009841198837 3 0.06441511966003131 4 0.12077834936255871 5 0.10288526056810557 6 0.093938716170879 7 0.1064638783269962 8 0.09483337061060165 9 0.11809438604339073 10-11 0.1002012972489376 12-13 0.09125475285171103 14-15 0.08991277119212704 16-17 0.10914784164616417 18-19 0.08991277119212704 20-21 0.08007157235517781 22-23 0.07067770073808992 24-25 0.06396779244017 26-27 0.053679266383359424 28-29 0.06217848356072467 30-31 0.0469693580854395 32-33 0.04875866696488481 34-35 0.042048758666964885 36-37 0.04831133974502348 38-39 0.05099530306419145 40-41 0.06575710131961529 42-43 0.06173115634086334 44-45 0.05233728472377544 46-47 0.03802281368821293 48-49 0.04607470364571684 50-51 0.029970923730709014 52-53 0.0438380675464102 54-55 0.04875866696488481 56-57 0.051442630284052784 58-59 0.05815253858197271 60-61 0.05502124804294341 62-63 0.0594945202415567 64-65 0.04339074032654887 66-67 0.06352046522030866 68-69 0.05502124804294341 70-71 0.029523596510847683 72-73 0.02773428763140237 74-75 0.03354954148959964 76-77 0.04473272198613285 78-79 0.06352046522030866 80-81 0.04473272198613285 82-83 0.05591590248266608 84-85 0.059047193021695366 86-87 0.06307313800044732 88-89 0.08186088123462312 90-91 0.060836501901140684 92-93 0.07291433683739655 94-95 0.07872959069559382 96-97 0.08678148065309774 98-99 0.11362111384477745 100-101 0.11675240438380675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 111775.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.97629165734735 #Duplication Level Percentage of deduplicated Percentage of total 1 69.33981973377902 20.78550659807649 2 10.448874828388945 6.264370386938046 3 4.709604249985077 4.235294117647059 4 3.080045365009252 3.6931335271751284 5 1.9638273742016357 2.943413106687542 6 1.4295946994568136 2.5712368597629163 7 1.1311406912194832 2.373518228584209 8 0.9371455858652181 2.2473719525833147 9 0.7282277800990867 1.964661149630955 >10 5.727332418074375 33.081637217624696 >50 0.3939592908732764 7.917691791545516 >100 0.08953620247119919 4.576157459181391 >500 0.008953620247119919 1.740102885260568 >1k 0.011938160329493226 5.60590471930217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2102 1.8805636322970252 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1465 1.3106687541936926 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1435 1.283829121002013 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1264 1.1308432118094387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 788 0.7049876985014538 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 626 0.5600536792663834 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 531 0.47506150749273096 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 455 0.407067770073809 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 305 0.2728696041154104 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 277 0.24781927980317603 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 253 0.22634757324983223 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 249 0.22276895549094164 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 247 0.2209796466114963 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 211 0.18877208678148066 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 209 0.18698277790203532 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 180 0.1610377991500783 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 173 0.15477521807201966 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164 0.14672332811451577 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 160 0.14314471035562515 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 159 0.14225005591590248 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 146 0.13061954819950794 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 142 0.12704093044061732 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 142 0.12704093044061732 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 136 0.12167300380228137 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 136 0.12167300380228137 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 136 0.12167300380228137 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 129 0.11541042272422276 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 0.1019906061283829 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 114 0.1019906061283829 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 114 0.1019906061283829 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 114 0.1019906061283829 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 112 0.1002012972489376 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 112 0.1002012972489376 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.4732721986132855E-4 0.0 22-23 0.0 0.0 0.0 0.0013419816595839856 0.0 24-25 0.0 0.0 0.0 0.002236636099306643 0.0 26-27 0.0 0.0 0.0 0.0076045627376425855 0.0 28-29 0.0 0.0 0.0 0.020129724893759787 0.0 30-31 0.0 0.0 0.0 0.04652203086557817 0.0 32-33 0.0 0.0 0.0 0.0738089912771192 0.0 34-35 0.0 0.0 0.0 0.09751733392976963 0.0 36-37 0.0 0.0 0.0 0.1476179825542384 0.0 38-39 0.0 0.0 0.0 0.21203310221426974 0.0 40-41 0.0 0.0 0.0 0.2630284052784612 0.0 42-43 0.0 0.0 0.0 0.3122343994632073 0.0 44-45 0.0 0.0 0.0 0.3502572131514202 0.0 46-47 0.0 0.0 0.0 0.40214717065533434 0.0 48-49 0.0 0.0 0.0 0.44240662044285395 0.0 50-51 0.0 0.0 0.0 0.467009617535227 0.0 52-53 0.0 0.0 0.0 0.5278461194363677 0.0 54-55 0.0 0.0 0.0 0.6244687989264146 0.0 56-57 0.0 0.0 0.0 0.770744799821069 0.0 58-59 0.0 0.0 0.0 0.9608588682621337 0.0 60-61 0.0 0.0 0.0 1.1657347349586222 0.0 62-63 0.0 0.0 0.0 1.279803176023261 0.0 64-65 0.0 0.0 0.0 1.4055021248042943 0.0 66-67 0.0 0.0 0.0 1.6765824200402595 0.0 68-69 0.0 0.0 0.0 2.0621784835607246 0.0 70-71 0.0 0.0 0.0 2.4231715499888167 0.0 72-73 0.0 0.0 0.0 2.8517110266159698 0.0 74-75 0.0 0.0 0.0 3.253858197271304 0.0 76-77 0.0 0.0 0.0 3.5714605233728474 0.0 78-79 0.0 0.0 0.0 3.851487363006039 0.0 80-81 0.0 0.0 0.0 4.028181614851263 0.0 82-83 0.0 0.0 0.0 4.2965779467680605 0.0 84-85 0.0 0.0 0.0 4.97338403041825 0.0 86-87 0.0 0.0 0.0 5.961529859091925 0.0 88-89 0.0 0.0 0.0 6.920599418474614 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATG 15 6.594464E-4 95.027794 1 TGGTACT 20 0.0020667568 71.27084 2 TTAGATA 40 5.3594226E-4 47.513897 4 GTATAGT 60 0.0039301775 31.67593 1 CATGGGC 95 3.4560515E-5 30.008778 4 TGGGGTG 65 0.0058134585 29.239319 5 AGGGGTG 65 0.0058134585 29.239319 8 GCAGATC 65 0.0058134585 29.239319 8 TAGATAC 85 6.3598115E-4 27.949352 5 AGATACC 70 0.008347779 27.150795 6 GTAGGGG 70 0.008347779 27.150795 6 TGGGATA 35 0.008365395 27.150795 18-19 GAGTACT 830 0.0 26.046772 12-13 GTACTTT 840 0.0 25.736694 14-15 GCTGGTT 95 0.0012175449 25.007315 8 CTGGTTA 95 0.0012175449 25.007315 9 ATATAGG 100 0.0016415376 23.756948 2 TGATAGT 50 0.0016531383 23.740984 60-61 ACTTTTT 950 0.0 23.262016 16-17 GTGATAG 105 0.002180192 22.625666 2 >>END_MODULE