FastQCFastQC Report
Thu 26 May 2016
SRR936951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111775
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23472.0997539700290764No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23152.0711250279579514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15861.4189219414001342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14221.2721986132856185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.8329232833817937No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA6560.5868933124580631No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA6370.5698948781033326No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT3700.3310221426973831No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.2889733840304182No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3020.27018564079624247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.250503243122344No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT2440.21829568329232835No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.20845448445537912No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2310.2066651755759338No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC2210.19771863117870725No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2160.19324535898009393No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT1980.17714157906508612No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG1940.17356296130619547No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT1710.15298590919257438No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA1680.1503019458734064No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG1650.1476179825542384No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC1540.1377767837172892No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG1510.13509282039812123No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT1510.13509282039812123No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG1480.13240885707895325No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA1470.13151420263923058No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG1430.12793558488034No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1420.12704093044061732No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA1380.12346231268172668No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG1310.11719973160366809No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.11630507716394542No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.1127264594050548No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1240.11093715052560948No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1210.10825318720644152No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1170.10467456944755088No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1140.1019906061283829No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA1130.10109595168866026No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC301.2965966E-463.328563
GTGGTAC352.7780727E-454.281621
TACTCCC405.369191E-447.496425
GCAGATC405.369191E-447.496428
AGCAGAT405.369191E-447.496427
TGGTACT405.369191E-447.496422
GGATATG652.5696736E-643.842851
AAGCAGA459.591315E-442.219046
ACTCCCG459.591315E-442.219046
CATGGGT500.001610175637.9971354
CTCCCGC500.001610175637.9971357
CCCGCTG550.002570663634.542859
GATCAGT352.0489699E-433.92601410-11
GATTGGC851.6199441E-533.5268829
AGGCTTA1051.8825431E-631.6642826
ATATGAG902.3948987E-531.664283
GATATGA953.4647965E-529.9977382
CTATTCA804.469983E-429.6852659
TTTTCGA404.484171E-429.68526516-17
GGGAAGC1302.9975854E-729.2285679