##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936951_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 111775 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23983896220085 33.0 31.0 34.0 31.0 34.0 2 32.248749720420484 33.0 31.0 34.0 30.0 34.0 3 32.37845672109148 34.0 31.0 34.0 31.0 34.0 4 36.10010288526057 37.0 35.0 37.0 35.0 37.0 5 36.057204204875866 37.0 35.0 37.0 35.0 37.0 6 36.13537016327444 37.0 35.0 37.0 35.0 37.0 7 36.079525833146946 37.0 35.0 37.0 35.0 37.0 8 36.09698948781033 37.0 35.0 37.0 35.0 37.0 9 37.76427644822187 39.0 37.0 39.0 35.0 39.0 10-11 37.73028852605681 39.0 37.0 39.0 35.0 39.0 12-13 37.655535674345785 39.0 37.0 39.0 35.0 39.0 14-15 38.804151196600316 40.0 38.0 41.0 35.0 41.0 16-17 38.67262357414448 40.0 38.0 41.0 34.5 41.0 18-19 38.723113397450234 40.0 38.0 41.0 35.0 41.0 20-21 38.80640125251621 40.0 38.0 41.0 35.0 41.0 22-23 38.70619995526728 40.0 38.0 41.0 35.0 41.0 24-25 38.59348244240662 40.0 38.0 41.0 35.0 41.0 26-27 38.375347796913445 40.0 37.5 41.0 34.0 41.0 28-29 38.19131737866249 40.0 37.0 41.0 34.0 41.0 30-31 37.93980317602326 40.0 37.0 41.0 34.0 41.0 32-33 37.74549317825989 40.0 35.5 41.0 33.5 41.0 34-35 37.59987922165064 40.0 35.0 41.0 33.0 41.0 36-37 37.339727130395886 40.0 35.0 41.0 33.0 41.0 38-39 37.10327890852159 39.5 35.0 41.0 33.0 41.0 40-41 36.876855289644375 39.0 35.0 41.0 33.0 41.0 42-43 36.56164169089689 39.0 35.0 41.0 31.5 41.0 44-45 36.08051442630284 39.0 35.0 41.0 30.5 41.0 46-47 35.58282710802952 39.0 35.0 41.0 29.0 41.0 48-49 35.06443301274882 39.0 35.0 41.0 24.5 41.0 50-51 34.75576828450011 39.0 35.0 40.5 23.0 41.0 52-53 34.51299038246478 39.0 35.0 40.0 22.0 41.0 54-55 34.28448221874301 38.5 35.0 40.0 20.0 41.0 56-57 34.065394766271524 38.0 34.5 40.0 19.0 41.0 58-59 33.77499440840975 38.0 34.0 40.0 14.0 41.0 60-61 33.46259897114739 38.0 34.0 40.0 9.0 41.0 62-63 33.10331469469917 37.5 33.0 40.0 4.5 41.0 64-65 32.69828226347573 37.0 33.0 39.5 2.0 41.0 66-67 32.29360769402818 36.5 33.0 39.0 2.0 40.5 68-69 31.829818832475958 36.0 32.0 39.0 2.0 40.0 70-71 31.327944531424734 35.5 31.0 38.5 2.0 40.0 72-73 30.93600984119884 35.0 31.0 38.0 2.0 40.0 74-75 30.47958398568553 35.0 31.0 37.0 2.0 39.0 76-77 29.051151867591145 33.5 28.5 35.5 2.0 38.5 78-79 29.636135092820396 34.0 30.0 36.0 2.0 39.0 80-81 29.382719749496758 34.0 30.0 36.0 2.0 37.5 82-83 28.991831804965333 34.0 29.5 35.5 2.0 37.0 84-85 28.541239096399018 34.0 29.0 35.0 2.0 37.0 86-87 28.16512636993961 34.0 29.0 35.0 2.0 36.0 88-89 27.71641690896891 34.0 28.5 35.0 2.0 36.0 90-91 27.23942294788638 34.0 26.5 35.0 2.0 35.5 92-93 26.80689778573026 34.0 26.0 35.0 2.0 35.0 94-95 26.299923954372623 33.0 25.0 35.0 2.0 35.0 96-97 25.70713039588459 33.0 22.5 35.0 2.0 35.0 98-99 25.11583538358309 33.0 12.0 35.0 2.0 35.0 100-101 22.40639677924402 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03346497752953326 1101 2 -0.21732275471531182 1101 3 -0.10731943427767732 1101 4 -0.16028534473957023 1101 5 -0.04808973220633561 1101 6 0.15473223916397671 1101 7 0.23243272252562264 1101 8 0.021998347944531815 1101 9 0.3185410598682381 1101 10-11 -0.03959401555079012 1101 12-13 0.3149671336434068 1101 14-15 0.2517659298130255 1101 16-17 0.21770006535369646 1101 18-19 0.25054972394872976 1101 20-21 0.42579840351194065 1101 22-23 0.05202403942874412 1101 24-25 0.2575105041665111 1101 26-27 0.18411144529399337 1101 28-29 0.08026433208658545 1101 30-31 0.6598013775084439 1101 32-33 0.6518131630655333 1101 34-35 0.31228143185851565 1101 36-37 0.7213206861478767 1101 38-39 0.0011637230560594958 1101 40-41 -0.02667069148446899 1101 42-43 0.7614381932786145 1101 44-45 0.3980865263132074 1101 46-47 0.4518227566729678 1101 48-49 0.7303261842714832 1101 50-51 0.10669564368096474 1101 52-53 0.3020248818871707 1101 54-55 0.37201795774674906 1101 56-57 -0.07704095166045732 1101 58-59 0.10345801341669159 1101 60-61 0.3755330414019582 1101 62-63 0.1340127353931635 1101 64-65 -0.04570895765237282 1101 66-67 -0.11926775327336259 1101 68-69 0.2887480796658721 1101 70-71 0.06733054470948474 1101 72-73 0.05871659481278968 1101 74-75 0.12330010413566228 1101 76-77 0.1426369316213112 1101 78-79 0.34965026838601787 1101 80-81 0.34976423412565083 1101 82-83 0.5392097053532723 1101 84-85 0.3026047518869106 1101 86-87 0.09335598824523217 1101 88-89 0.22420345720243873 1101 90-91 0.7449361077541319 1101 92-93 0.9468159296882419 1101 94-95 0.8586516655827587 1101 96-97 0.08536959806798095 1101 98-99 0.14755359809680613 1101 100-101 0.9926992742254548 1102 1 0.22688734459354976 1102 2 0.023811997057734402 1102 3 0.04790820241873206 1102 4 0.1524577346472924 1102 5 0.4483641685028843 1102 6 0.3728173455469559 1102 7 0.13165486848764374 1102 8 0.12992559571392093 1102 9 0.40587805369116126 1102 10-11 0.29974480851692675 1102 12-13 0.2742155893743643 1102 14-15 0.45952731164783955 1102 16-17 0.48906245382315916 1102 18-19 0.3916215610569367 1102 20-21 0.34280824107771224 1102 22-23 0.43614666583002304 1102 24-25 0.6512419157428013 1102 26-27 0.4760068925600649 1102 28-29 0.7177614723290944 1102 30-31 0.6498780190092504 1102 32-33 0.7068062996475533 1102 34-35 0.8596962110852431 1102 36-37 1.1687011482846899 1102 38-39 1.361779142868457 1102 40-41 1.551257700187918 1102 42-43 1.4162654639260666 1102 44-45 1.5246307953591867 1102 46-47 1.5249181581829276 1102 48-49 0.795385667227329 1102 50-51 0.9365400728733633 1102 52-53 1.1169127793934663 1102 54-55 0.9455423228805842 1102 56-57 0.9329681995635255 1102 58-59 0.6098352726251122 1102 60-61 0.8187668551745517 1102 62-63 0.5241122549539128 1102 64-65 0.9045312619770058 1102 66-67 1.258592004219203 1102 68-69 1.357468052212198 1102 70-71 1.43798371332079 1102 72-73 0.892192916020754 1102 74-75 0.4672145401914882 1102 76-77 0.6222182631243953 1102 78-79 0.7573086989511104 1102 80-81 0.9000673684198617 1102 82-83 0.9020271384349492 1102 84-85 0.7569630223055803 1102 86-87 0.9587758006451388 1102 88-89 0.6929749053836325 1102 90-91 0.7536869656813785 1102 92-93 0.6155988168994071 1102 94-95 0.5598928003345343 1102 96-97 1.238023453021082 1102 98-99 1.260371031178483 1102 100-101 2.083496574614383 1103 1 0.08333146295989735 1103 2 -0.10697792712910825 1103 3 0.002469219782391008 1103 4 0.15250664636165112 1103 5 -0.02050352530978472 1103 6 0.028870170198459277 1103 7 0.3328776613465365 1103 8 0.10336653481883928 1103 9 0.1678736516368744 1103 10-11 0.10250832037802127 1103 12-13 0.1696667999392929 1103 14-15 0.37760019010223544 1103 16-17 0.5473163056206616 1103 18-19 0.5167621822357091 1103 20-21 0.36322888182117197 1103 22-23 0.7158727602296366 1103 24-25 0.7073436521086691 1103 26-27 0.3708467066955592 1103 28-29 0.4728116068363093 1103 30-31 0.5910861383538304 1103 32-33 0.630650760395902 1103 34-35 0.7974315987105811 1103 36-37 1.0670381499965984 1103 38-39 0.957059162433147 1103 40-41 0.6006096199727082 1103 42-43 0.8502591054032038 1103 44-45 0.9320409200840629 1103 46-47 0.550376705505002 1103 48-49 0.3013773522358889 1103 50-51 0.1988535969623868 1103 52-53 0.505222879662476 1103 54-55 0.08934276308600886 1103 56-57 0.08508363120940743 1103 58-59 0.12044911464027308 1103 60-61 0.1742816509681404 1103 62-63 0.3628503327235322 1103 64-65 0.35273375647444283 1103 66-67 0.7188023245909321 1103 68-69 0.9441151541931276 1103 70-71 0.7659123022178278 1103 72-73 0.820145960774969 1103 74-75 1.1828662887852772 1103 76-77 1.0491441618771482 1103 78-79 1.0537381438031481 1103 80-81 1.4647531106551277 1103 82-83 1.4259449667548303 1103 84-85 1.346764929862438 1103 86-87 1.4698298480895033 1103 88-89 1.6163057931312466 1103 90-91 1.7588960632602486 1103 92-93 1.7014877873078191 1103 94-95 1.816410286272415 1103 96-97 2.0273606992915596 1103 98-99 2.2773923077742246 1103 100-101 1.8417259705547089 1104 1 0.39894083384697154 1104 2 0.3780970926129328 1104 3 0.5896763795612756 1104 4 0.3775132270407795 1104 5 0.3870247716104558 1104 6 0.4085490330571062 1104 7 0.37636278901171494 1104 8 0.49731231002284204 1104 9 0.49195893723834416 1104 10-11 0.4466778386723007 1104 12-13 0.4318410563225541 1104 14-15 0.7931042701232869 1104 16-17 0.4576348104903616 1104 18-19 0.6884652619935352 1104 20-21 0.5286671550509752 1104 22-23 0.5933931603349265 1104 24-25 0.6830214620586545 1104 26-27 0.49982801756683415 1104 28-29 0.842145642108747 1104 30-31 0.6484433376167971 1104 32-33 0.7725038796378101 1104 34-35 0.4671078292975821 1104 36-37 1.0626159336238743 1104 38-39 0.48361878338602793 1104 40-41 0.5253792962032904 1104 42-43 0.8985349674721661 1104 44-45 1.1701562135823451 1104 46-47 1.3609584375397503 1104 48-49 1.3685002793219638 1104 50-51 1.1686877638484034 1104 52-53 1.0652913187253858 1104 54-55 1.02472049406785 1104 56-57 1.3784503066503149 1104 58-59 1.3896252136136908 1104 60-61 1.638430111089228 1104 62-63 1.798596581736426 1104 64-65 1.5669480233867858 1104 66-67 1.9164069573890359 1104 68-69 1.8409827509291077 1104 70-71 2.2538301753423298 1104 72-73 2.202246513552012 1104 74-75 1.9391705793880654 1104 76-77 1.8664118639039984 1104 78-79 1.5610821817078637 1104 80-81 1.5321392649062453 1104 82-83 1.6335522318245843 1104 84-85 1.8786943849822073 1104 86-87 1.584675860197951 1104 88-89 1.3154371434866015 1104 90-91 1.4396541574955712 1104 92-93 1.736707581990629 1104 94-95 1.8214379251247443 1104 96-97 1.8782161875779515 1104 98-99 2.0644152085375858 1104 100-101 1.0616785896650036 1105 1 0.3646533020403595 1105 2 0.23267724528469103 1105 3 0.24241174851802327 1105 4 0.13756411762602028 1105 5 -0.1730897322063356 1105 6 0.2213989058306467 1105 7 0.3509811096223956 1105 8 0.30882630493378116 1105 9 0.028218479223077964 1105 10-11 0.05825544681479755 1105 12-13 0.3176553056864151 1105 14-15 0.6771691556194739 1105 16-17 0.629931248149397 1105 18-19 0.6863024121207673 1105 20-21 0.6747231346947373 1105 22-23 0.7220509211491688 1105 24-25 0.33076319233854434 1105 26-27 0.784783488304754 1105 28-29 0.5049955148822889 1105 30-31 0.5311723452503827 1105 32-33 0.508668001775213 1105 34-35 0.9276040125036857 1105 36-37 0.5959174603414255 1105 38-39 0.4073465187549843 1105 40-41 0.14026479238649614 1105 42-43 0.656868300805499 1105 44-45 1.4849144833024468 1105 46-47 0.8686238319417896 1105 48-49 0.8959175821209442 1105 50-51 1.320405321100317 1105 52-53 1.5931539141452333 1105 54-55 1.5583082803273953 1105 56-57 1.442985930059983 1105 58-59 1.6695870456747528 1105 60-61 1.102011536025607 1105 62-63 0.9075342407695146 1105 64-65 1.0268716875089225 1105 66-67 0.574549451027714 1105 68-69 0.6734254990207091 1105 70-71 0.6170617275051811 1105 72-73 0.9221574550278433 1105 74-75 0.9679237600496435 1105 76-77 0.844115426244965 1105 78-79 0.911209408170965 1105 80-81 1.2782588577815623 1105 82-83 1.0440484150306908 1105 84-85 0.7997821712417519 1105 86-87 0.8219850205032948 1105 88-89 0.9970529195680271 1105 90-91 0.7258500862487516 1105 92-93 0.6165739942043729 1105 94-95 0.654484998916093 1105 96-97 0.7931652969927114 1105 98-99 0.29405897444089035 1105 100-101 0.6568121774512612 1106 1 0.5519340037947416 1106 2 0.44583514002152924 1106 3 0.39811350290398906 1106 4 0.16738867902952848 1106 5 0.13085763621471358 1106 6 0.18894276547976574 1106 7 0.3575600569908204 1106 8 0.33821226984606056 1106 9 0.5413763739599204 1106 10-11 0.16965895558672628 1106 12-13 0.34353249866887836 1106 14-15 0.3344059977247369 1106 16-17 0.7455014235879887 1106 18-19 0.37139013141901245 1106 20-21 0.3554248890807017 1106 22-23 0.5051649562368894 1106 24-25 0.5033509116367938 1106 26-27 0.5428975233924689 1106 28-29 0.8909604271629874 1106 30-31 0.6765670820924896 1106 32-33 0.3751153701962622 1106 34-35 0.7210250651352581 1106 36-37 1.1505227234993214 1106 38-39 0.7104166941935759 1106 40-41 1.063949002912814 1106 42-43 0.7722191779984868 1106 44-45 0.3129846587410441 1106 46-47 0.09647470913476752 1106 48-49 0.22442635405077027 1106 50-51 0.0796158474161075 1106 52-53 0.03789075625049776 1106 54-55 0.42672933295897053 1106 56-57 -0.08968950853651592 1106 58-59 0.16563967725370077 1106 60-61 0.34433609742912097 1106 62-63 -0.3505797943182145 1106 64-65 -0.41260199670160347 1106 66-67 0.12279506506280313 1106 68-69 -0.21867976413718182 1106 70-71 -0.04697336021411758 1106 72-73 -0.30635131690198136 1106 74-75 0.0714325319794682 1106 76-77 -0.021454749193630107 1106 78-79 0.5884023906271061 1106 80-81 0.8304518402377035 1106 82-83 0.49273262555700725 1106 84-85 0.24912427650490798 1106 86-87 0.322642915240138 1106 88-89 0.22556169149785177 1106 90-91 0.2596220160733118 1106 92-93 0.7869687310464748 1106 94-95 0.6125990340038108 1106 96-97 1.5304459987470977 1106 98-99 2.21949757093212 1106 100-101 2.627206914293371 1107 1 0.14091116367557532 1107 2 0.21663950943563037 1107 3 0.23565074222871374 1107 4 0.2249855012738209 1107 5 0.4424763055295102 1107 6 0.5273737485979382 1107 7 0.048622619056359895 1107 8 -0.007368663619679694 1107 9 0.09897319620421285 1107 10-11 0.053224000273914385 1107 12-13 0.21309555725874674 1107 14-15 0.23023519335531972 1107 16-17 0.07246269469028022 1107 18-19 0.33127096557988267 1107 20-21 0.13730175104694098 1107 22-23 -0.055857883882275416 1107 24-25 0.5056845759863435 1107 26-27 0.22527090968839758 1107 28-29 0.4232345085929765 1107 30-31 0.48093649619377743 1107 32-33 0.36597932252993104 1107 34-35 0.6780757106168878 1107 36-37 0.24992689430369097 1107 38-39 0.6175666445411494 1107 40-41 0.4487553584242292 1107 42-43 0.5258934580382046 1107 44-45 0.5597572505980466 1107 46-47 0.33694144200468656 1107 48-49 0.34041443746685474 1107 50-51 0.5963487173267339 1107 52-53 0.16956900848485645 1107 54-55 0.23849695957267159 1107 56-57 0.17828467219834465 1107 58-59 0.15496440416531954 1107 60-61 0.40664990080548336 1107 62-63 -0.1612550674065929 1107 64-65 0.2627207441126984 1107 66-67 0.49781989127928483 1107 68-69 0.5668217254358083 1107 70-71 0.35605543819700713 1107 72-73 0.5493587128894788 1107 74-75 0.78459042671631 1107 76-77 1.2468669985720098 1107 78-79 0.9722156974791432 1107 80-81 0.7991708074670996 1107 82-83 0.9388597357854103 1107 84-85 0.40379160520401314 1107 86-87 0.5953340771070721 1107 88-89 0.6849460013290347 1107 90-91 1.2892148661229683 1107 92-93 1.8328475791100303 1107 94-95 1.0732743070921948 1107 96-97 0.9741401397600065 1107 98-99 1.4009772134346044 1107 100-101 1.6730857623569229 1108 1 0.13820402667803933 1108 2 -0.3390618851500946 1108 3 -0.27099404858342524 1108 4 0.10423078429268884 1108 5 0.2562580938806178 1108 6 -0.2604851521403688 1108 7 -0.05662758602977647 1108 8 0.021507464354073136 1108 9 -0.04352065121170767 1108 10-11 -0.2598604952141841 1108 12-13 -0.3450258537338726 1108 14-15 -0.26902649655443867 1108 16-17 -0.27967020112596686 1108 18-19 0.0029690787874372404 1108 20-21 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-2.0603674014078237 2316 86-87 -2.785814124795852 2316 88-89 -2.976237678722569 2316 90-91 -3.1055601701614997 2316 92-93 -3.4803277151973404 2316 94-95 -3.1039551720240794 2316 96-97 -2.704056925229512 2316 98-99 -2.5243170939351742 2316 100-101 -2.9648758567367715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 4.0 6 11.0 7 20.0 8 47.0 9 80.0 10 149.0 11 253.0 12 421.0 13 627.0 14 781.0 15 1117.0 16 1507.0 17 2231.0 18 1877.0 19 946.0 20 756.0 21 741.0 22 785.0 23 863.0 24 950.0 25 1085.0 26 1258.0 27 1371.0 28 1713.0 29 1979.0 30 2481.0 31 2958.0 32 3644.0 33 4690.0 34 6719.0 35 9781.0 36 13343.0 37 23433.0 38 21176.0 39 1977.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.91563461951464 24.92393872145465 18.530317130789605 17.630109528241107 2 14.322907347099454 25.80252657195004 41.27599040904698 18.598575671903518 3 16.61611688183876 31.591378801299086 34.38995803920516 17.402546277656995 4 10.693005529013904 22.62153989299837 42.1046039329361 24.580850645051623 5 9.658862158124055 40.71645209487085 37.74346219570021 11.881223551304878 6 23.137967129807734 42.98265234001056 19.806214380932783 14.073166149248925 7 21.10847685081637 38.514873630060386 26.092596734511297 14.284052784611944 8 22.321628271080296 35.85417132632521 23.43994632073362 18.38425408186088 9 24.342652650413775 19.330798479087452 25.784835607246702 30.541713263252067 10-11 20.235294117647058 30.24200402594498 30.581525385819724 18.941176470588236 12-13 23.54146350191907 29.632379912859097 30.39553380512288 16.43062278009895 14-15 21.345112950123017 30.294788637888615 30.795795124133303 17.564303287855065 16-17 16.34041601431447 33.29635428315813 32.44061731156341 17.92261239096399 18-19 15.677477074479981 31.02706329680161 37.01364348020577 16.281816148512636 20-21 16.8934696835494 30.167485304774942 37.127251254797756 15.8117937568779 22-23 17.223439946320735 29.480205770521135 36.6222321628271 16.674122120331024 24-25 17.057939376588056 30.388200980567586 37.12020899688652 15.433650645957842 26-27 17.16843216547973 29.869734817306657 37.56307483090578 15.39875818630784 28-29 16.084153831701283 29.415369749891518 38.686516688064486 15.813959730342708 30-31 16.594111146407048 30.26437650722349 37.658775787782936 15.482736558586533 32-33 16.32728834333873 31.186869816503094 36.90605065624077 15.579791183917404 34-35 16.87997387527232 29.591622193492974 37.48137942140885 16.04702450982585 36-37 17.60914007595854 29.554940212844954 36.59966986217417 16.236249849022336 38-39 16.993522528003435 30.176609526536165 37.13720788748523 15.692660057975164 40-41 16.407127231733448 30.02187557317133 37.84116276052734 15.72983443456788 42-43 16.233420564092242 30.728488671184774 36.696861929365454 16.341228835357533 44-45 16.603219904539092 30.58954045458002 35.691375862793954 17.115863778086933 46-47 17.411317378662492 31.09326772534109 34.20219190337732 17.2932229926191 48-49 17.499597401900264 30.989317730420314 34.588097410846885 16.922987456832537 50-51 17.651242686134523 31.18793279295722 34.247231019736255 16.91359350117201 52-53 17.231877250665413 31.80299268603637 34.454807756827485 16.51032230647073 54-55 17.32008016318935 31.40768707726443 33.99778119743765 17.274451562108577 56-57 17.91472077585127 32.049045395172406 32.762986025372626 17.2732478036037 58-59 17.385605711550593 31.00063968650888 33.823759656805954 17.78999494513458 60-61 17.322161881670887 32.091381639573065 33.21821906900593 17.36823740975012 62-63 18.05815253858197 31.422053231939163 33.85819727130396 16.661596958174904 64-65 18.135987474837844 30.978304629836728 33.78751957056587 17.098188324759562 66-67 17.246778111286567 31.6431744270831 33.41325615413175 17.69679130749858 68-69 17.12860657571013 31.261462760008946 33.98792216506374 17.622008499217177 70-71 17.664136274373853 31.82613207842576 33.2168786261625 17.292853021037892 72-73 17.637664951912324 30.358309103108926 33.40102885260568 18.602997092373073 74-75 17.579222359403797 31.5757689623705 33.1611108128903 17.68389786533541 76-77 18.086129538754715 30.969773694122487 33.46947174419697 17.474625022925828 78-79 17.344373467890563 30.424785728344695 33.44919211981319 18.781648683951545 80-81 17.612822990480282 32.265496385369694 32.16215732588934 17.95952329826068 82-83 17.646269397833976 31.7057568342556 31.99652863207526 18.65144513583516 84-85 17.00851297972184 30.744248258708694 32.126545018587194 20.120693742982272 86-87 17.600876800572603 31.206495481792967 32.145029972264474 19.047597745369956 88-89 18.25038247161658 29.727214980361982 32.73867570880266 19.283726839218772 90-91 17.290169045712574 29.44857726941297 33.26519018201826 19.9960635028562 92-93 17.624323973714933 29.990740285127643 32.66934765979718 19.715588081360238 94-95 17.32998134637149 29.71294883895701 32.93998183843363 20.01708797623787 96-97 17.739237018215654 29.66038005940665 31.850552911283685 20.749830011094012 98-99 17.656716217606615 30.06115209290466 31.555732505446425 20.7263991840423 100-101 17.196908018931225 30.505578270244154 32.13298381541159 20.164529895413025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2126.0 1 1324.5 2 495.0 3 424.0 4 346.5 5 302.5 6 366.0 7 587.5 8 814.0 9 864.5 10 702.0 11 536.0 12 473.5 13 437.5 14 424.5 15 390.5 16 370.5 17 378.0 18 393.5 19 427.5 20 454.0 21 503.0 22 556.5 23 557.0 24 642.0 25 778.0 26 991.5 27 1283.5 28 1409.0 29 1557.0 30 1933.0 31 2287.0 32 2947.0 33 4006.5 34 4846.0 35 5398.5 36 6289.5 37 7009.5 38 6802.5 39 6304.0 40 5598.0 41 4748.0 42 3931.0 43 3794.0 44 3768.0 45 3219.5 46 2747.5 47 2687.5 48 4396.0 49 4030.0 50 1729.5 51 924.0 52 433.0 53 274.5 54 211.0 55 182.5 56 164.5 57 155.0 58 153.0 59 164.5 60 167.0 61 150.0 62 131.0 63 99.0 64 65.0 65 47.5 66 28.5 67 23.5 68 19.5 69 12.5 70 12.5 71 9.5 72 5.0 73 4.0 74 4.0 75 4.0 76 2.5 77 1.5 78 1.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020577052113621114 2 0.0026839633191679713 3 0.003578617758890629 4 8.946544397226572E-4 5 0.0017893088794453144 6 0.0017893088794453144 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0017893088794453144 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.003578617758890629 22-23 0.0 24-25 0.0026839633191679713 26-27 0.0026839633191679713 28-29 0.0031312905390293 30-31 0.019235070454037127 32-33 0.0017893088794453144 34-35 0.0031312905390293 36-37 0.0031312905390293 38-39 0.0026839633191679713 40-41 0.005815253858197271 42-43 0.002236636099306643 44-45 0.0013419816595839856 46-47 0.0 48-49 8.946544397226572E-4 50-51 8.946544397226572E-4 52-53 0.002236636099306643 54-55 0.0026839633191679713 56-57 8.946544397226572E-4 58-59 0.0013419816595839856 60-61 0.0017893088794453144 62-63 0.0 64-65 0.0 66-67 4.473272198613286E-4 68-69 0.0 70-71 4.473272198613286E-4 72-73 0.0 74-75 8.946544397226572E-4 76-77 0.0013419816595839856 78-79 8.946544397226572E-4 80-81 0.0062625810780586 82-83 0.0031312905390293 84-85 0.004025944978751957 86-87 0.004473272198613286 88-89 0.0017893088794453144 90-91 4.473272198613286E-4 92-93 4.473272198613286E-4 94-95 4.473272198613286E-4 96-97 0.0026839633191679713 98-99 0.004025944978751957 100-101 0.0017893088794453144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 111775.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.390069335719076 #Duplication Level Percentage of deduplicated Percentage of total 1 68.0679418901459 19.324535898009394 2 10.931837519301673 6.207112502795796 3 5.1050956417609425 4.348020577052114 4 2.9496108152396556 3.349586222321628 5 2.0577947247345034 2.9210467456944755 6 1.5977058582548138 2.721538805636323 7 1.2605174424101093 2.50503243122344 8 0.923329026565405 2.097070006709908 9 0.7468565846279898 1.9082979199284276 >10 5.82674187754073 32.28271080295236 >50 0.3844578199350834 7.648400805188996 >100 0.12605174424101093 5.8349362558711695 >500 0.00945388081807582 1.9897114739431894 >1k 0.012605174424101093 6.86199955267278 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2347 2.0997539700290764 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2315 2.0711250279579514 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1586 1.4189219414001342 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1422 1.2721986132856185 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.8329232833817937 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 656 0.5868933124580631 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 637 0.5698948781033326 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 370 0.3310221426973831 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 323 0.2889733840304182 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 302 0.27018564079624247 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.250503243122344 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 244 0.21829568329232835 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.20845448445537912 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 231 0.2066651755759338 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 221 0.19771863117870725 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 216 0.19324535898009393 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 198 0.17714157906508612 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 194 0.17356296130619547 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 171 0.15298590919257438 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 168 0.1503019458734064 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 165 0.1476179825542384 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 154 0.1377767837172892 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 151 0.13509282039812123 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 151 0.13509282039812123 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 148 0.13240885707895325 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 147 0.13151420263923058 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 143 0.12793558488034 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 142 0.12704093044061732 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 138 0.12346231268172668 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 131 0.11719973160366809 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.11630507716394542 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.1127264594050548 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 124 0.11093715052560948 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 121 0.10825318720644152 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 117 0.10467456944755088 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 114 0.1019906061283829 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 113 0.10109595168866026 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.946544397226571E-4 0.0 12-13 0.0 0.0 0.0 8.946544397226571E-4 0.0 14-15 0.0 0.0 0.0 8.946544397226571E-4 0.0 16-17 0.0 0.0 0.0 8.946544397226571E-4 0.0 18-19 0.0 0.0 0.0 8.946544397226571E-4 0.0 20-21 0.0 0.0 0.0 0.0013419816595839856 0.0 22-23 0.0 0.0 0.0 0.002236636099306643 0.0 24-25 0.0 0.0 0.0 0.0031312905390292996 0.0 26-27 0.0 0.0 0.0 0.008946544397226572 0.0 28-29 0.0 0.0 0.0 0.0219190337732051 0.0 30-31 0.0 0.0 0.0 0.04965332140460747 0.0 32-33 0.0 0.0 0.0 0.07917691791545516 0.0 34-35 0.0 0.0 0.0 0.10422724222768956 0.0 36-37 0.0 0.0 0.0 0.15701185417132635 0.0 38-39 0.0 0.0 0.0 0.22187430105121897 0.0 40-41 0.0 0.0 0.0 0.27286960411541045 0.0 42-43 0.0 0.0 0.0 0.3256542160590472 0.0 44-45 0.0 0.0 0.0 0.3654663386267054 0.0 46-47 0.0 0.0 0.0 0.41825095057034223 0.0 48-49 0.0 0.0 0.0 0.45761574591813914 0.0 50-51 0.0 0.0 0.0 0.48311339745023485 0.0 52-53 0.0 0.0 0.0 0.5439498993513755 0.0 54-55 0.0 0.0 0.0 0.6410199060612838 0.0 56-57 0.0 0.0 0.0 0.7899798702751062 0.0 58-59 0.0 0.0 0.0 0.9850145381346455 0.0 60-61 0.0 0.0 0.0 1.1934690225900246 0.0 62-63 0.0 0.0 0.0 1.30977409975397 0.0 64-65 0.0 0.0 0.0 1.439051666293894 0.0 66-67 0.0 0.0 0.0 1.7128159248490271 0.0 68-69 0.0 0.0 0.0 2.0997539700290764 0.0 70-71 0.0 0.0 0.0 2.46477298143592 0.0 72-73 0.0 0.0 0.0 2.89554909416238 0.0 74-75 0.0 0.0 0.0 3.3008275553567437 0.0 76-77 0.0 0.0 0.0 3.6237978080966227 0.0 78-79 0.0 0.0 0.0 3.9056139566092596 0.0 80-81 0.0 0.0 0.0 4.083650190114069 0.0 82-83 0.0 0.0 0.0 4.3556251397897565 0.0 84-85 0.0 0.0 0.0 5.03377320509953 0.0 86-87 0.0 0.0 0.0 6.032207559830016 0.0 88-89 0.0 0.0 0.0 7.010512189666741 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACTC 30 1.2965966E-4 63.32856 3 GTGGTAC 35 2.7780727E-4 54.28162 1 TACTCCC 40 5.369191E-4 47.49642 5 GCAGATC 40 5.369191E-4 47.49642 8 AGCAGAT 40 5.369191E-4 47.49642 7 TGGTACT 40 5.369191E-4 47.49642 2 GGATATG 65 2.5696736E-6 43.84285 1 AAGCAGA 45 9.591315E-4 42.21904 6 ACTCCCG 45 9.591315E-4 42.21904 6 CATGGGT 50 0.0016101756 37.997135 4 CTCCCGC 50 0.0016101756 37.997135 7 CCCGCTG 55 0.0025706636 34.54285 9 GATCAGT 35 2.0489699E-4 33.926014 10-11 GATTGGC 85 1.6199441E-5 33.526882 9 AGGCTTA 105 1.8825431E-6 31.664282 6 ATATGAG 90 2.3948987E-5 31.66428 3 GATATGA 95 3.4647965E-5 29.997738 2 CTATTCA 80 4.469983E-4 29.685265 9 TTTTCGA 40 4.484171E-4 29.685265 16-17 GGGAAGC 130 2.9975854E-7 29.228567 9 >>END_MODULE