##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936950_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96427 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09415412695614 33.0 31.0 34.0 31.0 34.0 2 32.239621682723715 34.0 31.0 34.0 31.0 34.0 3 32.19678098457901 34.0 31.0 34.0 30.0 34.0 4 35.7833179503666 37.0 35.0 37.0 35.0 37.0 5 35.78519501799288 37.0 35.0 37.0 35.0 37.0 6 35.89435531541996 37.0 36.0 37.0 35.0 37.0 7 35.85603617244133 37.0 36.0 37.0 35.0 37.0 8 35.83040019911436 37.0 36.0 37.0 35.0 37.0 9 37.516639530421976 39.0 38.0 39.0 35.0 39.0 10-11 37.385125535379096 39.0 37.0 39.0 35.0 39.0 12-13 37.36295332220229 39.0 37.0 39.0 35.0 39.0 14-15 38.53763468738009 40.0 38.0 41.0 34.5 41.0 16-17 38.43943605006896 40.0 38.0 41.0 34.5 41.0 18-19 38.429366256338994 40.0 38.0 41.0 35.0 41.0 20-21 38.395807190932004 40.0 38.0 41.0 34.5 41.0 22-23 38.32747052174184 40.0 38.0 41.0 34.0 41.0 24-25 38.17621620500482 40.0 38.0 41.0 34.0 41.0 26-27 38.17343690045319 40.0 38.0 41.0 34.0 41.0 28-29 38.083477656672926 40.0 38.0 41.0 34.0 41.0 30-31 37.877160961141584 40.0 37.0 41.0 34.0 41.0 32-33 37.58540139172638 40.0 35.5 41.0 33.5 41.0 34-35 37.37426758065687 40.0 35.0 41.0 33.0 41.0 36-37 37.18715193877233 40.0 35.0 41.0 33.0 41.0 38-39 36.96314828834248 39.0 35.0 41.0 33.0 41.0 40-41 36.76848289379531 39.0 35.0 41.0 33.0 41.0 42-43 36.50390450807346 39.0 35.0 41.0 31.5 41.0 44-45 36.131762887987804 39.0 35.0 41.0 31.0 41.0 46-47 35.63926078795358 39.0 35.0 41.0 29.5 41.0 48-49 35.1939964947577 39.0 35.0 41.0 27.0 41.0 50-51 34.43468115776702 38.0 34.0 40.0 24.5 40.5 52-53 34.59547637072604 38.5 35.0 40.0 24.0 40.5 54-55 34.95230588942931 39.0 35.0 41.0 24.0 41.0 56-57 34.851716842792996 39.0 35.0 41.0 23.0 41.0 58-59 34.64048969686914 39.0 35.0 41.0 22.0 41.0 60-61 34.39007228265942 39.0 35.0 41.0 19.0 41.0 62-63 34.04994970288405 38.0 35.0 40.0 14.0 41.0 64-65 33.70491667271615 38.0 35.0 40.0 7.5 41.0 66-67 33.29658705549276 37.5 34.0 40.0 2.0 41.0 68-69 32.899110207721904 37.0 34.0 39.5 2.0 41.0 70-71 32.426192871291235 36.5 33.5 39.0 2.0 41.0 72-73 31.941484231594885 36.0 33.0 39.0 2.0 40.5 74-75 31.433747809223558 35.0 33.0 38.0 2.0 40.0 76-77 30.94956288176548 35.0 32.0 37.0 2.0 39.5 78-79 30.47560330612795 35.0 31.5 37.0 2.0 39.0 80-81 29.994062866209674 35.0 31.0 36.0 2.0 39.0 82-83 29.540859925124707 35.0 31.0 36.0 2.0 37.5 84-85 29.11776265983594 35.0 30.5 35.5 2.0 37.0 86-87 28.7198035819843 35.0 30.0 35.0 2.0 37.0 88-89 28.29602704636668 34.0 29.0 35.0 2.0 36.0 90-91 27.913089694795026 34.0 29.0 35.0 2.0 36.0 92-93 27.56128988768706 34.0 28.5 35.0 2.0 36.0 94-95 27.165156024764848 34.0 27.0 35.0 2.0 35.0 96-97 26.726974809959867 34.0 25.5 35.0 2.0 35.0 98-99 26.226933327802378 34.0 24.5 35.0 2.0 35.0 100-101 24.263577628672465 31.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3906374869317162 1101 2 0.13042317802836578 1101 3 -0.17500251048123516 1101 4 -0.3368178396123369 1101 5 0.09214754696645144 1101 6 -0.23781384351512003 1101 7 -0.22259275355474273 1101 8 -0.022204743898512902 1101 9 0.1349684915691185 1101 10-11 -0.22719698275466982 1101 12-13 0.237739922952656 1101 14-15 -0.3518141395519976 1101 16-17 0.24769764633964542 1101 18-19 0.029285468772215495 1101 20-21 -0.10627937029244805 1101 22-23 0.35681889942953404 1101 24-25 0.03759378268635061 1101 26-27 0.22174331857890195 1101 28-29 0.22331996857936787 1101 30-31 -0.07202283197254644 1101 32-33 -0.30597975737356364 1101 34-35 -0.11885675346772473 1101 36-37 -0.1802096307803538 1101 38-39 0.15990084163034624 1101 40-41 0.490357674156634 1101 42-43 0.33927983688511176 1101 44-45 0.6839826832232845 1101 46-47 0.390892328343341 1101 48-49 0.6879881698352364 1101 50-51 0.717265735596726 1101 52-53 0.39119187498071284 1101 54-55 -0.01062964076432138 1101 56-57 0.01234689904901387 1101 58-59 -0.4964169225097308 1101 60-61 -0.09838824390013912 1101 62-63 -0.3279470805209783 1101 64-65 0.14049874647935212 1101 66-67 -0.09170621034542137 1101 68-69 0.3718212520124027 1101 70-71 -0.014676717217994906 1101 72-73 0.24087602826635646 1101 74-75 -0.05193254901281463 1101 76-77 0.38462277595282046 1101 78-79 0.7574415059107622 1101 80-81 0.20944480110802388 1101 82-83 0.3546889003207596 1101 84-85 0.5351839236616414 1101 86-87 0.2961142919026436 1101 88-89 1.0379479558865796 1101 90-91 0.7674958126338929 1101 92-93 0.8943524861223473 1101 94-95 0.34542308786780396 1101 96-97 0.217032519286267 1101 98-99 -0.04276190928081647 1101 100-101 0.04391293857775125 1102 1 0.4028220952425343 1102 2 0.5442400640562965 1102 3 0.2034100419643039 1102 4 0.21723699112506267 1102 5 0.19353268404329782 1102 6 0.4928028585941959 1102 7 0.034146139485088156 1102 8 0.20786594981951367 1102 9 0.47027511838675196 1102 10-11 0.490545416233779 1102 12-13 0.725354153655573 1102 14-15 0.8030223453034537 1102 16-17 0.7700839903824033 1102 18-19 0.7661956360232054 1102 20-21 0.8504422803771305 1102 22-23 0.8007524588294999 1102 24-25 0.620788219148217 1102 26-27 0.5803271452356 1102 28-29 0.4133308887471898 1102 30-31 0.6444838638145711 1102 32-33 0.25310409802042244 1102 34-35 0.48890806060204994 1102 36-37 0.07782244002831362 1102 38-39 -0.0749402411905038 1102 40-41 -0.34914459608878445 1102 42-43 0.44100982136697553 1102 44-45 0.4345717975401939 1102 46-47 0.15372352553858093 1102 48-49 0.29845428857770173 1102 50-51 0.25028487462770244 1102 52-53 0.21250344461115134 1102 54-55 0.21329127423079086 1102 56-57 0.6856327200732082 1102 58-59 0.4394989347234457 1102 60-61 0.19579532986846715 1102 62-63 0.13314263727889397 1102 64-65 0.09066829645348662 1102 66-67 -0.27180276761883704 1102 68-69 -0.5623991054794075 1102 70-71 -0.39863556542766787 1102 72-73 0.2667970582106065 1102 74-75 0.045285681919995824 1102 76-77 0.011124299028860918 1102 78-79 0.017427712014562502 1102 80-81 -0.3233157023691895 1102 82-83 -0.2435351355433717 1102 84-85 0.029695102465023382 1102 86-87 -0.24224998190619473 1102 88-89 -1.0786392042950403 1102 90-91 -0.739717245587844 1102 92-93 -0.2466038906809196 1102 94-95 -0.08270496546859007 1102 96-97 0.6343553539319373 1102 98-99 0.58454425774028 1102 100-101 0.019400035537344706 1103 1 0.1788780961998171 1103 2 0.3570915657913645 1103 3 0.08793798261286412 1103 4 0.19635629230970153 1103 5 0.3057140888536338 1103 6 0.08966776226776574 1103 7 0.2603038515827265 1103 8 0.21337031741554568 1103 9 0.028669278970696155 1103 10-11 0.6034148669936243 1103 12-13 0.29182537538585507 1103 14-15 0.5448555390332643 1103 16-17 0.4625872810369458 1103 18-19 0.6339711535534249 1103 20-21 0.5327744569955328 1103 22-23 0.6854299746844745 1103 24-25 0.6814559228696382 1103 26-27 0.5982761700052492 1103 28-29 0.8133548207802193 1103 30-31 0.5851025068682958 1103 32-33 1.1198625041470223 1103 34-35 0.8780130025668669 1103 36-37 0.4515510507738014 1103 38-39 0.49257800698467946 1103 40-41 0.9473732931059047 1103 42-43 1.2493740669096312 1103 44-45 1.699020762479769 1103 46-47 1.849876451229619 1103 48-49 2.2407182589019783 1103 50-51 1.7704476124522799 1103 52-53 1.80709480514723 1103 54-55 2.056267222537599 1103 56-57 1.9765265815067394 1103 58-59 2.146122124863446 1103 60-61 2.1360250773855256 1103 62-63 2.3127525451405617 1103 64-65 2.197165843420102 1103 66-67 2.0196594795990706 1103 68-69 2.045953174046744 1103 70-71 2.448857554112834 1103 72-73 2.3410761057156932 1103 74-75 2.3217604934552334 1103 76-77 2.1086772486519294 1103 78-79 2.4659738410082177 1103 80-81 2.3057078706833423 1103 82-83 2.4303310843920123 1103 84-85 2.2001510076941706 1103 86-87 2.0901958718690814 1103 88-89 2.212015336808225 1103 90-91 2.0563818330288868 1103 92-93 1.897676353425819 1103 94-95 2.0612162981427495 1103 96-97 2.3392733867188227 1103 98-99 2.7751030707114417 1103 100-101 2.0515287898750287 1104 1 -0.5424333792100171 1104 2 -0.6915135535643984 1104 3 -0.26092598057379135 1104 4 -0.597143851492433 1104 5 -0.8065953733264095 1104 6 -0.7143299368981815 1104 7 -1.0333400943476718 1104 8 -1.0421660645229096 1104 9 -1.0239622999766738 1104 10-11 -0.3599155902971489 1104 12-13 -0.7249275725577746 1104 14-15 -0.4700279812428363 1104 16-17 -0.6552585630815457 1104 18-19 -0.3908347135734118 1104 20-21 -0.22901731752272525 1104 22-23 -0.07269484556287154 1104 24-25 -0.5594845430492512 1104 26-27 -0.4428512386803902 1104 28-29 -0.36388130231440385 1104 30-31 0.0704346897846051 1104 32-33 0.08592520187495012 1104 34-35 0.42769663788467227 1104 36-37 0.42149640596505833 1104 38-39 0.602227129791693 1104 40-41 1.0882993788119748 1104 42-43 1.1057876418305455 1104 44-45 0.6038956546281753 1104 46-47 0.6136095544795452 1104 48-49 1.1145318913437379 1104 50-51 0.7494525017246403 1104 52-53 1.2396516069878984 1104 54-55 1.3755079473003207 1104 56-57 1.2109240505892842 1104 58-59 1.22650751456721 1104 60-61 1.6933302255017182 1104 62-63 1.7389820533372884 1104 64-65 2.00957596708993 1104 66-67 1.5392165690439867 1104 68-69 1.6554584947254867 1104 70-71 2.221578290379739 1104 72-73 2.1249415069236335 1104 74-75 2.0317116007316223 1104 76-77 1.887869080691047 1104 78-79 2.396445511988947 1104 80-81 1.7693978332947893 1104 82-83 1.537966977403233 1104 84-85 1.982794090811801 1104 86-87 2.3099974249320674 1104 88-89 1.9950898263106716 1104 90-91 2.344992704469785 1104 92-93 2.5378115274263955 1104 94-95 2.767788055404761 1104 96-97 2.4106711434056223 1104 98-99 2.560981701715182 1104 100-101 2.381770377450522 1105 1 -0.33243337921001626 1105 2 -0.08028548338896258 1105 3 0.24924945802269605 1105 4 0.4037333414900246 1105 5 -0.09887607508079554 1105 6 -0.2883650246174838 1105 7 -0.2552699189090788 1105 8 -0.23023623996150633 1105 9 -0.20133072102930072 1105 10-11 -0.059126116612937096 1105 12-13 -0.5169451164174177 1105 14-15 -0.10555429703230601 1105 16-17 0.044390559725471235 1105 18-19 -0.04004523988920283 1105 20-21 -0.17919275611921393 1105 22-23 -0.10129133679094338 1105 24-25 0.2773575622139006 1105 26-27 -0.010658256224253648 1105 28-29 0.02646957487858259 1105 30-31 -0.11588109968907645 1105 32-33 -0.14653093847592658 1105 34-35 -0.24247880071181527 1105 36-37 -0.005170260701611085 1105 38-39 0.5225780069846806 1105 40-41 0.6991765717944318 1105 42-43 0.22798062428668686 1105 44-45 -0.2029464506349825 1105 46-47 -0.7033202700818606 1105 48-49 -0.5036260033931086 1105 50-51 -1.3353720596788676 1105 52-53 -1.0768396210822786 1105 54-55 -0.7129131053312534 1105 56-57 -1.2536373529194904 1105 58-59 -1.1793696784152417 1105 60-61 -0.508073283270214 1105 62-63 -0.736017946662713 1105 64-65 -0.8821784188749788 1105 66-67 -0.2090290449910981 1105 68-69 0.01300235437461339 1105 70-71 -0.6899129376904369 1105 72-73 0.0359941385025806 1105 74-75 0.11995721476671051 1105 76-77 0.3026936420945532 1105 78-79 0.1342525295328123 1105 80-81 0.08562590347023047 1105 82-83 -0.4759803910178242 1105 84-85 -0.17501292673205882 1105 86-87 -0.3744762592784632 1105 88-89 -0.18429613860160998 1105 90-91 -0.2663230850039007 1105 92-93 0.3732501239176216 1105 94-95 0.5650687571591426 1105 96-97 0.07435535393193504 1105 98-99 -0.8986674210918402 1105 100-101 -0.6219138330757943 1106 1 0.06745673068009239 1106 2 0.16235187924840488 1106 3 0.3037549525281946 1106 4 -0.306706218949536 1106 5 -0.5210738772785959 1106 6 0.006799810547349239 1106 7 0.10681799317883645 1106 8 0.2517417820165164 1106 9 -0.19660544630402654 1106 10-11 -0.13478545727227953 1106 12-13 0.17409883962653794 1106 14-15 0.5090061425281291 1106 16-17 0.10548946082437283 1106 18-19 0.08154816670420928 1106 20-21 -0.07391803084448867 1106 22-23 0.16178558628597983 1106 24-25 0.07021470507105221 1106 26-27 0.14313295256695824 1106 28-29 0.02646957487858259 1106 30-31 0.31609692228894204 1106 32-33 0.290282248337256 1106 34-35 0.10477394654092365 1106 36-37 0.08175281622146713 1106 38-39 0.08323734764402246 1106 40-41 0.7358798684977259 1106 42-43 0.7164971078031641 1106 44-45 0.9906799229913901 1106 46-47 1.355415993654404 1106 48-49 0.7991761944090925 1106 50-51 1.3141334348266227 1106 52-53 1.588215323972669 1106 54-55 0.7021418397236943 1106 56-57 0.8643846251024883 1106 58-59 1.0487072446616779 1106 60-61 0.513794848597918 1106 62-63 0.3049161192713541 1106 64-65 0.9039754272788656 1106 66-67 0.8041577681957222 1106 68-69 0.4944309258031794 1106 70-71 0.3146475018699988 1106 72-73 -0.3303794878710491 1106 74-75 -0.38432849951900394 1106 76-77 0.19132001572092605 1106 78-79 0.8132085734888541 1106 80-81 0.7386478814922057 1106 82-83 0.4123163122788789 1106 84-85 0.6264705897514595 1106 86-87 1.1963479165457152 1106 88-89 1.507187377881909 1106 90-91 0.9340615303807134 1106 92-93 1.0099534206209206 1106 94-95 1.0262226033129913 1106 96-97 0.639849859426441 1106 98-99 0.37166224923783275 1106 100-101 1.114130122968163 1107 1 0.5187911105859015 1107 2 0.2588981900804299 1107 3 -0.04789339912015578 1107 4 0.12108028026553797 1107 5 0.464797394306963 1107 6 -0.10387522869911692 1107 7 0.22534232598888337 1107 8 0.6379270253446165 1107 9 0.07968968713396407 1107 10-11 0.6344453119584941 1107 12-13 0.46295284276625637 1107 14-15 0.8347518254166673 1107 16-17 0.9186762740111831 1107 18-19 0.8484241478659058 1107 20-21 0.5579501010236356 1107 22-23 0.8509535611682466 1107 24-25 0.8763371540506384 1107 26-27 0.8578111315308519 1107 28-29 0.5060614116132811 1107 30-31 0.8808535941884728 1107 32-33 1.25653028601387 1107 34-35 0.9442558931657317 1107 36-37 0.9640134127677698 1107 38-39 0.6405371906581507 1107 40-41 0.9544826942434028 1107 42-43 0.4676745018377062 1107 44-45 1.1221555901813431 1107 46-47 1.142700138081409 1107 48-49 1.09627195579057 1107 50-51 1.1053422260354182 1107 52-53 1.5666297666728255 1107 54-55 1.7468828130360947 1107 56-57 2.3506483613662184 1107 58-59 2.0644058317888394 1107 60-61 2.1421307983624374 1107 62-63 2.575716747214841 1107 64-65 2.532311377043385 1107 66-67 2.445052587661962 1107 68-69 2.5454513339664473 1107 70-71 2.4873319602687474 1107 72-73 1.98476964870666 1107 74-75 1.7024061943585487 1107 76-77 1.9095303767884317 1107 78-79 1.554966815247095 1107 80-81 1.5922585565314549 1107 82-83 1.5814685885740154 1107 84-85 1.67121156306386 1107 86-87 1.1633808835786823 1107 88-89 0.138663045071862 1107 90-91 0.20132997622058824 1107 92-93 0.17243379738701137 1107 94-95 0.12394630817955132 1107 96-97 -0.5595221970884694 1107 98-99 0.16561829319387655 1107 100-101 0.4744126975364509 1108 1 -0.15606974284638042 1108 2 0.17471451661103998 1108 3 -0.19075054197730168 1108 4 -0.10990302214634085 1108 5 -0.49160334780806636 1108 6 0.012544066291610534 1108 7 0.10382099018183055 1108 8 -0.4975089672342321 1108 9 0.21048746078887604 1108 10-11 0.16610115611433685 1108 12-13 0.46805488358258174 1108 14-15 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-1.5134796299376312 2316 96-97 -2.1613435707992465 2316 98-99 -1.7502803243176466 2316 100-101 -2.417182650280097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 351.0 3 71.0 4 68.0 5 66.0 6 63.0 7 65.0 8 104.0 9 121.0 10 126.0 11 194.0 12 295.0 13 434.0 14 552.0 15 831.0 16 1054.0 17 1590.0 18 1411.0 19 730.0 20 664.0 21 690.0 22 626.0 23 815.0 24 821.0 25 926.0 26 1027.0 27 1074.0 28 1236.0 29 1396.0 30 1642.0 31 2014.0 32 2647.0 33 3430.0 34 4853.0 35 8089.0 36 10983.0 37 18493.0 38 22232.0 39 4643.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.11494968357714 25.149911816578484 17.33893557422969 19.396202925614688 2 13.596896168967914 24.903264624417773 40.80417440376775 20.69566480284656 3 15.70823998008464 31.733881005725667 33.85196249273919 18.7059165214505 4 9.996680222425097 21.667773259191634 42.02527180678894 26.31027471159432 5 9.252129878485373 40.861498230722134 37.34265879398549 12.543713096807 6 21.63704464572893 43.604029839906204 20.15646237328934 14.602463141075523 7 20.391603457399892 38.5170120261899 25.851638944517656 15.239745571892543 8 21.127725145531333 35.82924323707339 23.8411970406035 19.201834576791775 9 23.717276943669134 19.314774271869844 25.274702470506448 31.693246313954575 10-11 19.63681643665041 30.17017744111238 30.47213863235447 19.720867489882743 12-13 22.55912170926335 30.141954362917534 29.813010407911257 17.485913519907854 14-15 20.50636851799009 30.924797011595633 30.15123608913331 18.417598381280968 16-17 15.380026249850854 34.17720205641125 31.795002152902786 18.647769540835117 18-19 15.222612421477443 32.25455054751814 35.56870820991913 16.954128821085284 20-21 16.324444905582073 31.649201079062045 34.943452998547414 17.082901016808467 22-23 16.723892905675253 30.72115434487194 34.61271148639537 17.942241263057436 24-25 16.48593284065863 31.83871385971736 34.80772721379489 16.86762608582912 26-27 16.376601151273142 31.476948607581807 35.299486594409586 16.846963646735468 28-29 15.522722440322154 30.743101329170706 36.61364850358093 17.12052772692621 30-31 15.868463768416577 31.990001503923164 35.38005175570065 16.761482971959612 32-33 15.403004578949279 32.62462468691499 35.02973983478446 16.942630899351272 34-35 15.998817629762542 31.39867138923317 35.11172880160554 17.490782179398753 36-37 16.626218626840906 31.488799004355943 34.61055797552375 17.274424393279403 38-39 16.171940632875945 31.869379881141292 34.79624962402896 17.162429861953804 40-41 15.312334705815244 31.986434209025195 35.82488928530684 16.876341799852725 42-43 15.559289162686746 32.235549125454384 34.254808315572774 17.950353396286097 44-45 15.727767695099818 32.414830178895514 33.97407311381903 17.883329012185637 46-47 16.483362630866637 32.408257237527806 32.87390652790525 18.234473603700305 48-49 16.398151941135904 32.34569693701355 33.07423866093512 18.181912460915424 50-51 16.528642767876125 32.298629440834674 33.23670796148122 17.936019829807975 52-53 16.106884875261223 33.08235196507003 33.09946433108799 17.711298828580762 54-55 16.279045648532744 32.580389228543424 32.90552418288452 18.23504094003931 56-57 16.718531196514704 33.322960427363725 31.69441419013537 18.264094185986206 58-59 16.25698787558211 31.879232915357253 33.084414574193346 18.779364634867296 60-61 16.75491777347903 32.45756426945198 32.69664611219733 18.09087184487167 62-63 17.117327359097224 32.41178666998569 32.90704669446346 17.563839276453628 64-65 17.075902696031402 31.82084536841614 33.16186041205358 17.94139152349888 66-67 16.240898624681062 32.37237330678117 32.60263032339702 18.784097745140745 68-69 15.863202825564931 32.52459662571119 33.08784249861263 18.524358050111246 70-71 16.639697950377563 32.02172018919592 32.793959007551244 18.544622852875282 72-73 16.712304612544212 31.140637479125825 32.56474883571377 19.582309072616194 74-75 16.34996369671196 32.05476610310134 32.71237423503786 18.882895965148844 76-77 16.54175894278794 31.25632102235891 33.4024863984565 18.799433636396643 78-79 16.16699689824372 30.712574042760664 33.47562683486001 19.644802224135603 80-81 16.179727493866796 32.23653158923876 32.442959912449496 19.14078100444495 82-83 16.398161787588954 31.7148696031038 32.15575011929708 19.731218490010168 84-85 16.061778464666162 30.69837878206392 32.02709290626394 21.212749847005984 86-87 16.45566994269118 31.520369265876617 32.29105619376086 19.732904597671343 88-89 17.267399647339488 29.535836531480136 32.76475469349652 20.432009127683852 90-91 16.29438702216045 29.1389423351191 33.6846090415463 20.882061601174147 92-93 16.409844143046083 29.924535152096677 33.5344000414927 20.131220663364537 94-95 16.07551279724088 29.181858257915614 33.55496201021705 21.187666934626456 96-97 16.034648201457507 28.95770118519671 32.83591379444488 22.171736818900907 98-99 16.00599712594483 29.43105120902267 32.61845101914827 21.944500645884236 100-101 15.883704883502551 29.957302426446557 32.985042879154975 21.173949810895923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1158.0 1 755.5 2 348.5 3 314.5 4 245.5 5 234.0 6 347.0 7 597.0 8 865.0 9 940.5 10 724.5 11 487.0 12 439.0 13 416.5 14 396.5 15 397.5 16 376.5 17 351.5 18 347.0 19 352.0 20 374.5 21 419.5 22 463.0 23 510.0 24 540.5 25 647.0 26 899.0 27 1129.5 28 1199.5 29 1317.0 30 1736.0 31 2107.0 32 2567.5 33 3436.5 34 4221.5 35 4691.5 36 5462.5 37 6213.5 38 6069.5 39 5431.5 40 4717.5 41 4014.5 42 3338.0 43 3191.0 44 3135.5 45 2763.0 46 2346.5 47 2198.5 48 3677.5 49 3426.5 50 1425.5 51 734.5 52 387.0 53 258.5 54 203.0 55 208.0 56 188.0 57 165.0 58 170.5 59 155.0 60 147.0 61 140.5 62 119.0 63 93.0 64 69.0 65 48.0 66 31.5 67 25.0 68 21.0 69 17.0 70 13.5 71 11.5 72 10.0 73 5.5 74 4.0 75 4.0 76 3.0 77 2.5 78 3.0 79 2.0 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03837099567548508 2 0.031111618115258175 3 0.019704024806330177 4 0.036296887801134534 5 0.06222323623051635 6 0.047704481110062535 7 0.05600091260746471 8 0.05807502048181526 9 0.051852696858763626 10-11 0.05911207441899053 12-13 0.0601491283561658 14-15 0.05651943957605235 16-17 0.0471859541414749 18-19 0.038889522644072715 20-21 0.048741535047237806 22-23 0.026963402366557084 24-25 0.01503728208904145 26-27 0.012444647246103271 28-29 0.014000228151866178 30-31 0.012963174214690907 32-33 0.007777904528814544 34-35 0.010889066340340361 36-37 0.007259377560226907 38-39 0.010370539371752724 40-41 0.00829643149740218 42-43 0.005703796654463999 44-45 0.002074107874350545 46-47 0.002592634842938181 48-49 0.0015555809057629089 50-51 0.006740850591639271 52-53 0.005703796654463999 54-55 0.005703796654463999 56-57 0.022815186617855997 58-59 0.010370539371752724 60-61 0.017111389963391996 62-63 0.013481701183278542 64-65 0.007777904528814544 66-67 0.013481701183278542 68-69 0.022296659649268358 70-71 0.019704024806330177 72-73 0.01659286299480436 74-75 0.01762991693197963 76-77 0.023333713586443632 78-79 0.031111618115258175 80-81 0.026444875397969445 82-83 0.0300745641780829 84-85 0.018666970869154906 86-87 0.020222551774917812 88-89 0.01762991693197963 90-91 0.017111389963391996 92-93 0.025407821460794174 94-95 0.020222551774917812 96-97 0.03059309114667054 98-99 0.049260062015825445 100-101 0.05340827776452653 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 96427.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.189397160546324 #Duplication Level Percentage of deduplicated Percentage of total 1 69.43640897755611 21.656797370031217 2 10.630091438071487 6.630922874298692 3 4.778054862842892 4.4707395231626 4 2.8162926018287617 3.513538739149823 5 2.024937655860349 3.157829238698705 6 1.5029093931837074 2.812490277619339 7 1.2335827098919367 2.6932290748441825 8 0.9875311720698254 2.4640401547284476 9 0.714879467996675 2.0066993684341523 >10 5.4397339983374895 31.804370145291255 >50 0.3325020781379884 6.9036680597757885 >100 0.0831255195344971 4.5785931326288285 >500 0.006650041562759767 1.255872317919255 >1k 0.013300083125519535 6.051209723417715 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1872 1.94136497039211 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 1.4819500762234645 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1392 1.4435790805479793 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1142 1.1843155962541612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 689 0.7145301627137627 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 522 0.5413421552054922 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 427 0.4428220311738414 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 381 0.39511755006377886 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 261 0.2706710776027461 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 253 0.26237464610534394 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 246 0.255115268545117 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 242 0.2509670527964159 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 213 0.22089248861833305 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 209 0.21674427286963194 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 186 0.1928920323146007 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 175 0.18148443900567268 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 158 0.16385452207369305 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 143 0.14829871301606398 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 138 0.1431134433301876 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 138 0.1431134433301876 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 128 0.13274290395843488 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 125 0.12963174214690906 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 123 0.1275576342725585 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 121 0.12548352639820795 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 118 0.12237236458668216 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 109 0.1130388791521047 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 108 0.11200182521492942 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.10992771734057889 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 105 0.10889066340340361 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101 0.10474244765470253 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 101 0.10474244765470253 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 99 0.10266833978035197 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.185269685876363E-4 0.0 12-13 0.0 0.0 0.0 0.0010370539371752725 0.0 14-15 0.0 0.0 0.0 0.0010370539371752725 0.0 16-17 0.0 0.0 0.0 0.0010370539371752725 0.0 18-19 0.0 0.0 0.0 0.0010370539371752725 0.0 20-21 0.0 0.0 0.0 0.002592634842938181 0.0 22-23 0.0 0.0 0.0 0.009852012403165088 0.0 24-25 0.0 0.0 0.0 0.01762991693197963 0.0 26-27 0.0 0.0 0.0 0.02644487539796945 0.0 28-29 0.0 0.0 0.0 0.04044510354983563 0.0 30-31 0.0 0.0 0.0 0.06377881713627925 0.0 32-33 0.0 0.0 0.0 0.09229780040859925 0.0 34-35 0.0 0.0 0.0 0.11977972974374397 0.0 36-37 0.0 0.0 0.0 0.15555809057629089 0.0 38-39 0.0 0.0 0.0 0.21259605712093088 0.0 40-41 0.0 0.0 0.0 0.27015255063415844 0.0 42-43 0.0 0.0 0.0 0.3163014508384581 0.0 44-45 0.0 0.0 0.0 0.3541539195453556 0.0 46-47 0.0 0.0 0.0 0.41534010183869663 0.0 48-49 0.0 0.0 0.0 0.449044354796893 0.0 50-51 0.0 0.0 0.0 0.47548923019486244 0.0 52-53 0.0 0.0 0.0 0.5304530888651519 0.0 54-55 0.0 0.0 0.0 0.6440104949858443 0.0 56-57 0.0 0.0 0.0 0.7923092080019082 0.0 58-59 0.0 0.0 0.0 0.9737936470075809 0.0 60-61 0.0 0.0 0.0 1.2071307828720172 0.0 62-63 0.0 0.0 0.0 1.3222437698984724 0.0 64-65 0.0 0.0 0.0 1.463801632322897 0.0 66-67 0.0 0.0 0.0 1.7396579796115197 0.0 68-69 0.0 0.0 0.0 2.137886691486824 0.0 70-71 0.0 0.0 0.0 2.5293745527704896 0.0 72-73 0.0 0.0 0.0 3.0328642392690845 0.0 74-75 0.0 0.0 0.0 3.485019755877503 0.0 76-77 0.0 0.0 0.0 3.818951123647941 0.0 78-79 0.0 0.0 0.0 4.111918860899955 0.0 80-81 0.0 0.0 0.0 4.312588797743371 0.0 82-83 0.0 0.0 0.0 4.614371493461375 0.0 84-85 0.0 0.0 0.0 5.368309705787798 0.0 86-87 0.0 0.0 0.0 6.476920364628164 0.0 88-89 0.0 0.0 0.0 7.528493056923891 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTACAC 25 0.004978983 57.08179 3 GGGATTC 25 0.0049892524 57.05211 7 CGCAGAA 35 2.7554913E-4 54.36361 8 TGGGATA 35 0.00837967 40.772705 6 AACTTGC 50 0.0016012563 38.03474 7 CTATTCA 55 0.0025499342 34.595024 9 AGATGGA 55 0.0025499342 34.595024 6 ATACTTA 85 1.6009655E-5 33.577526 6 ATGGGAT 210 0.0 31.712107 5 ACTTGCT 60 0.0039056053 31.712105 8 TGGGATT 90 2.366886E-5 31.712105 6 TTCAAGT 30 0.003945285 31.662685 80-81 CATTCAA 30 0.003945285 31.662685 78-79 AAACTTG 65 0.0057771853 29.272715 6 TCCTCGA 70 0.008295801 27.181805 8 CCTCGAA 70 0.008295801 27.181805 9 CTGGTCG 70 0.008295801 27.181805 9 CCTGGTC 70 0.008295801 27.181805 8 TCTATTC 70 0.008295801 27.181805 8 CCCTGGT 70 0.008316898 27.16767 7 >>END_MODULE