##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936950_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96427 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34529747892188 33.0 31.0 34.0 31.0 34.0 2 32.43164258973109 34.0 31.0 34.0 31.0 34.0 3 32.52390927852158 34.0 31.0 34.0 31.0 34.0 4 36.17461914194157 37.0 35.0 37.0 35.0 37.0 5 36.10174536177627 37.0 35.0 37.0 35.0 37.0 6 36.19128459871198 37.0 36.0 37.0 35.0 37.0 7 36.14750018148444 37.0 36.0 37.0 35.0 37.0 8 36.15532993871011 37.0 36.0 37.0 35.0 37.0 9 37.85269685876362 39.0 38.0 39.0 35.0 39.0 10-11 37.78018086220664 39.0 37.5 39.0 35.0 39.0 12-13 37.735903844358944 39.0 37.0 39.0 35.0 39.0 14-15 38.906893297520405 40.0 38.0 41.0 35.5 41.0 16-17 38.797541145114955 40.0 38.0 41.0 35.5 41.0 18-19 38.793092183724475 40.0 38.0 41.0 35.0 41.0 20-21 38.901993217667254 40.0 38.0 41.0 35.0 41.0 22-23 38.818728157051446 40.0 38.0 41.0 35.0 41.0 24-25 38.70885229240773 40.0 38.0 41.0 35.0 41.0 26-27 38.49084799900443 40.0 38.0 41.0 34.5 41.0 28-29 38.32030966430564 40.0 37.0 41.0 34.0 41.0 30-31 38.09968162444129 40.0 37.0 41.0 34.0 41.0 32-33 37.858748068487046 40.0 35.5 41.0 34.0 41.0 34-35 37.68959420079438 40.0 35.0 41.0 33.0 41.0 36-37 37.44162941914609 40.0 35.0 41.0 33.0 41.0 38-39 37.218030219751725 40.0 35.0 41.0 33.0 41.0 40-41 36.93765750256671 39.5 35.0 41.0 33.0 41.0 42-43 36.62628724319951 39.0 35.0 41.0 32.5 41.0 44-45 36.231812666576786 39.0 35.0 41.0 31.0 41.0 46-47 35.62628724319951 39.0 35.0 41.0 28.5 41.0 48-49 35.05762908728883 39.0 35.0 41.0 24.5 41.0 50-51 34.75382932166302 39.0 35.0 41.0 23.0 41.0 52-53 34.531894593837826 39.0 35.0 40.5 21.5 41.0 54-55 34.261213145695706 38.5 35.0 40.0 19.0 41.0 56-57 34.03614132971056 38.0 35.0 40.0 14.5 41.0 58-59 33.71174567289245 38.0 34.0 40.0 7.5 41.0 60-61 33.38689371234197 38.0 34.0 40.0 2.0 41.0 62-63 33.02745600298672 37.5 33.5 40.0 2.0 41.0 64-65 32.611986269405875 37.0 33.0 40.0 2.0 41.0 66-67 32.14952762193162 36.5 33.0 39.0 2.0 40.5 68-69 31.66136559262447 36.0 32.0 39.0 2.0 40.0 70-71 31.253518205481868 35.5 31.0 38.5 2.0 40.0 72-73 30.85296649278729 35.0 31.0 38.0 2.0 40.0 74-75 30.315814035487985 35.0 31.0 37.0 2.0 39.0 76-77 28.813439181971855 33.5 28.5 35.5 2.0 38.5 78-79 29.366577825712717 34.0 30.0 36.0 2.0 39.0 80-81 28.957019299573773 34.0 29.0 36.0 2.0 37.5 82-83 28.71916060854325 34.0 29.0 35.5 2.0 37.0 84-85 28.321476349984962 34.0 29.0 35.0 2.0 37.0 86-87 27.936433778920843 34.0 29.0 35.0 2.0 36.0 88-89 27.479767077685707 34.0 27.5 35.0 2.0 36.0 90-91 26.99681624441287 34.0 26.0 35.0 2.0 35.5 92-93 26.590581476142575 34.0 25.5 35.0 2.0 35.0 94-95 26.146094973399563 33.0 24.5 35.0 2.0 35.0 96-97 25.539086562892138 33.0 21.5 35.0 2.0 35.0 98-99 24.911051883808476 33.0 10.0 35.0 2.0 35.0 100-101 22.136346666390118 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19406354086465427 1101 2 -0.2972887324975275 1101 3 -0.53225386335507 1101 4 0.05440905428594789 1101 5 0.20632354600756742 1101 6 0.18781400353591238 1101 7 0.2673245962356319 1101 8 0.08506272523075609 1101 9 0.21113338001591586 1101 10-11 0.3318858426393234 1101 12-13 0.10395591695068873 1101 14-15 0.4200950799371057 1101 16-17 0.41446814406563703 1101 18-19 0.13592924270069773 1101 20-21 0.31711027776332656 1101 22-23 0.3489072872492258 1101 24-25 0.09035987933201284 1101 26-27 0.2820723953801263 1101 28-29 0.30603163114748355 1101 30-31 0.1771343533412093 1101 32-33 -0.0030288161592579854 1101 34-35 0.1971959989381702 1101 36-37 0.028787381950927227 1101 38-39 0.1840230949314403 1101 40-41 -0.425788190381688 1101 42-43 0.35957478824489186 1101 44-45 0.5500447476801966 1101 46-47 0.28400369914005097 1101 48-49 -0.20938830673215847 1101 50-51 -0.9874854840769771 1101 52-53 -1.0808997766612265 1101 54-55 -1.2368037226115334 1101 56-57 -0.7757053856100171 1101 58-59 -0.86313510367372 1101 60-61 -1.2611514012118619 1101 62-63 -1.2529058040404166 1101 64-65 -0.5306759498516591 1101 66-67 -0.674108596119968 1101 68-69 -0.3888220234664779 1101 70-71 -0.45174130663140843 1101 72-73 0.40593447326546794 1101 74-75 0.11035491923778196 1101 76-77 0.05401744305249423 1101 78-79 0.1620977139573121 1101 80-81 0.6675796080646457 1101 82-83 0.3481931729999381 1101 84-85 0.2103815301387506 1101 86-87 0.05012330531526388 1101 88-89 0.5394041637120743 1101 90-91 0.24699910809251335 1101 92-93 0.7835244272996356 1101 94-95 0.38391163322471655 1101 96-97 0.6363973759837798 1101 98-99 1.168452000902672 1101 100-101 1.541235459691265 1102 1 0.16457908773516294 1102 2 0.35159913462126724 1102 3 -0.025155754268340047 1102 4 0.08383603284793395 1102 5 -0.04236890758171796 1102 6 0.10240679622514648 1102 7 0.11449972124281516 1102 8 0.3031212343404732 1102 9 0.5159784860564898 1102 10-11 0.5210059070108386 1102 12-13 0.33279090746739115 1102 14-15 0.41630175848185047 1102 16-17 0.2572752019425266 1102 18-19 0.3750521808005942 1102 20-21 0.2606702524745117 1102 22-23 0.6814263975429213 1102 24-25 0.41397043166260517 1102 26-27 0.8014125873451832 1102 28-29 0.8820130093876131 1102 30-31 0.6706397272132705 1102 32-33 0.37409055851744455 1102 34-35 0.5355200405497555 1102 36-37 0.8029698062281838 1102 38-39 0.6144501309679882 1102 40-41 0.6836721234294387 1102 42-43 0.6601667762901897 1102 44-45 0.8322448741242496 1102 46-47 0.1651462935420227 1102 48-49 0.7996639376497043 1102 50-51 0.1453669787082461 1102 52-53 0.17770703982247937 1102 54-55 0.45073579115362605 1102 56-57 0.2465947465814864 1102 58-59 0.49177035066274044 1102 60-61 -0.16674942408673843 1102 62-63 0.40721834102539844 1102 64-65 0.4779452352739213 1102 66-67 0.2024360328817032 1102 68-69 0.46030723683584185 1102 70-71 0.2604260593091645 1102 72-73 0.7498622852086818 1102 74-75 0.43462642909467064 1102 76-77 0.8281423927622491 1102 78-79 0.4731500732883056 1102 80-81 0.08958661996188155 1102 82-83 0.3849481589186716 1102 84-85 0.306766379842756 1102 86-87 0.23820882747170913 1102 88-89 0.46951508962735744 1102 90-91 0.8064278108915275 1102 92-93 0.6861337726643129 1102 94-95 0.8191952702153458 1102 96-97 0.9901820762539053 1102 98-99 1.1050000783783887 1102 100-101 2.3625470293217035 1103 1 0.09176587725293217 1103 2 -0.03047577264210588 1103 3 -0.07517370329311035 1103 4 0.10346029992942363 1103 5 0.013991030194993925 1103 6 0.14560411583745037 1103 7 0.16870577604716885 1103 8 0.08557905413226763 1103 9 0.06456051309968558 1103 10-11 -0.022251242684028227 1103 12-13 0.04719200834090742 1103 14-15 0.48779870714763973 1103 16-17 0.043711722224919924 1103 18-19 -0.049561915166862036 1103 20-21 -0.05693774082451597 1103 22-23 -0.36033500008780806 1103 24-25 -0.22453620947656105 1103 26-27 0.06014777939974181 1103 28-29 0.08714044746347582 1103 30-31 0.20537109014255606 1103 32-33 0.18891645297718185 1103 34-35 -0.24071545065521605 1103 36-37 0.1268898732378787 1103 38-39 0.09808660338632791 1103 40-41 -0.05077205510352201 1103 42-43 -0.038708418157590074 1103 44-45 0.1661864799636632 1103 46-47 -0.14516242968401372 1103 48-49 -0.1345528865695158 1103 50-51 0.11619981345755548 1103 52-53 0.11201360916554393 1103 54-55 0.3300285995777088 1103 56-57 0.42079003197098075 1103 58-59 0.3064466699160562 1103 60-61 0.4051065050744427 1103 62-63 0.7227621964759123 1103 64-65 0.6857949421065186 1103 66-67 0.23127413267828345 1103 68-69 1.24097015415067 1103 70-71 1.1320007870822941 1103 72-73 1.311188119838338 1103 74-75 1.1536942764082987 1103 76-77 0.7751030245679189 1103 78-79 1.2538429056444151 1103 80-81 1.4900592776141472 1103 82-83 0.9402610702504859 1103 84-85 1.1152802005918296 1103 86-87 0.6064031555798834 1103 88-89 0.4884812258006228 1103 90-91 0.29969692660290903 1103 92-93 0.6271413112547144 1103 94-95 1.424346640324238 1103 96-97 0.3660346549304663 1103 98-99 0.6794885311724528 1103 100-101 1.530908881661059 1104 1 0.2866177610608176 1104 2 0.11879083650371314 1104 3 0.1465691785015366 1104 4 0.19966392374305997 1104 5 -0.10076306816566216 1104 6 0.15106859671176665 1104 7 0.05783433514984182 1104 8 0.28474500178828777 1104 9 0.35417799943848394 1104 10-11 0.4570412508448811 1104 12-13 0.35917072562592267 1104 14-15 0.39619675143870126 1104 16-17 0.3661879948737621 1104 18-19 0.042615249055167226 1104 20-21 -0.03155666518460265 1104 22-23 0.07020857195706043 1104 24-25 0.5317191831032559 1104 26-27 0.3338751305645431 1104 28-29 0.4706607235635616 1104 30-31 0.9442983262656455 1104 32-33 0.7968399502449444 1104 34-35 0.654165193578315 1104 36-37 0.3894725594616375 1104 38-39 0.8326278745971365 1104 40-41 0.5790812667256446 1104 42-43 0.6048780069215027 1104 44-45 0.9118005150513824 1104 46-47 1.3188151371839751 1104 48-49 1.0155046342817897 1104 50-51 1.0492599227471757 1104 52-53 1.2523644863585304 1104 54-55 0.8710841071992164 1104 56-57 0.8798064254136051 1104 58-59 1.1569643575778343 1104 60-61 1.1549627029461718 1104 62-63 1.0618476149401062 1104 64-65 1.2848027074214414 1104 66-67 1.407359838746732 1104 68-69 1.1714418251313923 1104 70-71 1.2637235365789827 1104 72-73 1.5142367249576942 1104 74-75 1.5274360077859264 1104 76-77 1.4332134646024315 1104 78-79 1.7050939841603778 1104 80-81 1.0499528640392306 1104 82-83 1.8701575327181317 1104 84-85 1.9790995851187212 1104 86-87 2.0654914500866397 1104 88-89 2.009116831207585 1104 90-91 1.5673126873161642 1104 92-93 1.9308514061641198 1104 94-95 3.0429114950840876 1104 96-97 2.5174582960003526 1104 98-99 3.0693361009164875 1104 100-101 2.968642560119502 1105 1 0.11012653299064112 1105 2 -0.027524952969969263 1105 3 -0.07500976886688449 1105 4 -0.032441339414837955 1105 5 0.21923693183433812 1105 6 0.10773526337843009 1105 7 -0.006376191165948342 1105 8 -0.14753569996609883 1105 9 -0.2624886672281832 1105 10-11 0.2828307245290915 1105 12-13 0.1239952870294303 1105 14-15 0.16812657600009828 1105 16-17 0.2559248369790197 1105 18-19 0.11191349466919576 1105 20-21 -0.21611806869336903 1105 22-23 -0.07058090172715481 1105 24-25 -0.39617555373885693 1105 26-27 -0.292966974698615 1105 28-29 -0.10802348696275743 1105 30-31 0.4031579753884529 1105 32-33 0.48850661691160724 1105 34-35 0.5872353690169092 1105 36-37 0.5750865945493544 1105 38-39 0.37587348863223013 1105 40-41 0.9588181088309042 1105 42-43 1.0126850244653625 1105 44-45 1.0261864799636626 1105 46-47 1.155657242447134 1105 48-49 0.5146274412993392 1105 50-51 0.2725932560805049 1105 52-53 0.48201360916554137 1105 54-55 0.7424876159711502 1105 56-57 0.8448064254136085 1105 58-59 0.8740696207357317 1105 60-61 1.5521556854023046 1105 62-63 1.005794983361163 1105 64-65 1.2074342863688088 1105 66-67 1.2995528212028695 1105 68-69 0.7292488426752577 1105 70-71 1.1899516067544234 1105 72-73 1.3977454968875165 1105 74-75 1.1478746042771526 1105 76-77 0.9195292540761173 1105 78-79 1.7235150367919552 1105 80-81 1.569075671056769 1105 82-83 1.906736480086554 1105 84-85 1.3508539710836338 1105 86-87 1.2668949588585718 1105 88-89 1.7519238487514457 1105 90-91 1.6411723364389736 1105 92-93 2.6542724587956954 1105 94-95 2.097297459996369 1105 96-97 2.368247769684565 1105 98-99 2.3641606623199927 1105 100-101 2.438203963628272 1106 1 0.3165001593642671 1106 2 -0.06686561231062882 1106 3 0.10839682453971022 1106 4 0.24217404520054941 1106 5 -0.33153229893489566 1106 6 0.24630669194986154 1106 7 0.19230512751537532 1106 8 0.2460906736602766 1106 9 0.36333550859599484 1106 10-11 0.05305050474886741 1106 12-13 0.19009418812832735 1106 14-15 0.052741960615485084 1106 16-17 0.42576000181419005 1106 18-19 0.5586167913725006 1106 20-21 0.5175083049330027 1106 22-23 0.6774960213497678 1106 24-25 0.5881101605468615 1106 26-27 0.6025275307958964 1106 28-29 0.37335013941087425 1106 30-31 0.48266346989394293 1106 32-33 0.06515496855995906 1106 34-35 0.2338837206652613 1106 36-37 0.2559657154284807 1106 38-39 0.254499862258605 1106 40-41 0.9490928341056346 1106 42-43 0.3183443651247089 1106 44-45 -0.0618354980583149 1106 46-47 -0.01577132898143674 1106 48-49 0.3019900786619729 1106 50-51 -0.24378037029311628 1106 52-53 0.19443119158312072 1106 54-55 0.06985025333379014 1106 56-57 0.371564667171846 1106 58-59 0.6529157745818921 1106 60-61 0.6997381029847318 1106 62-63 0.7726631152292924 1106 64-65 0.19732439625892084 1106 66-67 -0.28962300297295585 1106 68-69 0.33545763388404737 1106 70-71 -0.05548795368514092 1106 72-73 -0.34049626135424305 1106 74-75 0.3484789998815465 1106 76-77 0.19579299033985365 1106 78-79 -0.23143001815309816 1106 80-81 -0.3998254278443305 1106 82-83 -0.4103514320013595 1106 84-85 -0.7724427322130722 1106 86-87 -0.3044237224601112 1106 88-89 0.030275497103094295 1106 90-91 -0.2102562349895969 1106 92-93 0.08498674450998323 1106 94-95 0.7203743830732883 1106 96-97 0.9651159015526964 1106 98-99 0.8288309919903227 1106 100-101 2.139357809782119 1107 1 0.1595142880926801 1107 2 0.11288321029533677 1107 3 0.10682696582699691 1107 4 0.19429335446271523 1107 5 0.30740019714045985 1107 6 0.266714855215163 1107 7 -0.11617210953329504 1107 8 0.19899491227879906 1107 9 -0.13901927947307513 1107 10-11 0.14527970412092372 1107 12-13 0.3910361033559653 1107 14-15 0.3109837188572371 1107 16-17 0.4650064696320797 1107 18-19 0.5170155354855268 1107 20-21 0.6081676455923457 1107 22-23 0.6778884860279533 1107 24-25 0.725865262587682 1107 26-27 0.7296860865258736 1107 28-29 0.47656834977194507 1107 30-31 0.45244368967416904 1107 32-33 0.7339147801769101 1107 34-35 0.6322353690169109 1107 36-37 0.69120904352895 1107 38-39 0.3961796110812017 1107 40-41 0.4227977006676369 1107 42-43 0.702174820383739 1107 44-45 0.5794517860861177 1107 46-47 0.5301470383655058 1107 48-49 0.38911723721770386 1107 50-51 0.17851162342744686 1107 52-53 0.08650340508390997 1107 54-55 -0.06751238402885207 1107 56-57 -0.06407112560680162 1107 58-59 0.371518600327569 1107 60-61 0.31276793030026795 1107 62-63 0.3805909017285103 1107 64-65 -0.09113714220261926 1107 66-67 0.2539405763049096 1107 68-69 0.1717998630834181 1107 70-71 0.39270670879523806 1107 72-73 0.18815366015282464 1107 74-75 -0.281819273273868 1107 76-77 0.05136598876999443 1107 78-79 0.5378007510776719 1107 80-81 0.6276470996282022 1107 82-83 0.29255280661716654 1107 84-85 0.8084049914917948 1107 86-87 0.7799561833483679 1107 88-89 1.4941687467106313 1107 90-91 1.2081111119491759 1107 92-93 1.1360071526732511 1107 94-95 1.8381137865269785 1107 96-97 2.0271253207049718 1107 98-99 1.876711682728157 1107 100-101 1.446265188118069 1108 1 -0.29850983064572745 1108 2 0.11520231975730155 1108 3 -0.019100677957787582 1108 4 -0.22198679396029064 1108 5 0.06969147728888458 1108 6 0.10182617246933745 1108 7 -0.024103463893219157 1108 8 0.20200975457935755 1108 9 0.051147696408186505 1108 10-11 -0.011260184561820097 1108 12-13 0.09013165066578921 1108 14-15 -0.18414615127262834 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2316 98-99 1.9830316300619302 2316 100-101 0.3792254690046164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 8.0 6 7.0 7 19.0 8 30.0 9 66.0 10 104.0 11 251.0 12 324.0 13 510.0 14 648.0 15 990.0 16 1376.0 17 2031.0 18 1803.0 19 942.0 20 710.0 21 659.0 22 747.0 23 744.0 24 876.0 25 992.0 26 1000.0 27 1149.0 28 1365.0 29 1706.0 30 1957.0 31 2426.0 32 3000.0 33 3906.0 34 5467.0 35 7973.0 36 11612.0 37 20636.0 38 18795.0 39 1598.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.13404286218128 25.28578245264621 18.37513744528122 17.20503723989129 2 14.336977038435212 25.985770882163823 40.997905042417706 18.67934703698326 3 16.77366494155716 31.77382051255458 34.2266565718375 17.225857974050758 4 10.71587833283209 22.86911342258911 41.936387111493666 24.47862113308513 5 9.844226421356122 40.795669038186304 37.37425068967663 11.985853850780941 6 22.999782218673193 43.25033444989474 19.69261721302125 14.05726611841082 7 21.073973057338712 38.61055513497257 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16.42644122299733 30.6615492336817 37.722050776691475 15.189958766629497 32-33 16.18694031205115 31.575863481511824 36.8005683085044 15.436627897932622 34-35 16.84772744950608 29.907438616505484 37.286940288833 15.957893645155435 36-37 17.563320679569358 29.836953139585535 36.630053726637215 15.969672454207895 38-39 16.757241679717076 30.629219775774985 37.243961377708175 15.369577166799765 40-41 16.286366229321164 30.55074417881035 37.87118187004097 15.291707721827516 42-43 16.31101592520185 31.053884328377556 36.48017257919819 16.1549271672224 44-45 16.537895129843193 30.96117149257446 35.73902762797644 16.76190574960591 46-47 17.443970836833536 31.330180558580423 34.15886251205625 17.06698609252979 48-49 17.4865569792223 31.486484384317425 34.498654401584645 16.52830423487563 50-51 17.54021176641397 31.870223070301883 33.86032957574123 16.72923558754291 52-53 17.113744198708808 32.15069096943141 34.36905286629158 16.366511965568204 54-55 17.105952263664694 31.819902411706448 33.83026274170214 17.243882582926716 56-57 17.881921038713223 32.231117840438884 32.687421572796 17.199539548051895 58-59 17.397887488268147 31.297219096608263 33.62077458763502 17.68411882748858 60-61 17.168946456905225 32.25341450008815 33.232911943750196 17.34472709925643 62-63 17.91821792651436 31.743702489966502 33.577732377860976 16.760347205658167 64-65 17.97548302790828 31.35870072493077 33.70772533524159 16.958090911919356 66-67 17.310863365309825 31.789473684210527 33.267824734249416 17.631838216230232 68-69 16.984615065516902 31.814198526323427 33.826114461423586 17.37507194673608 70-71 17.47386542769435 31.982701402140545 33.32417240521032 17.219260764954782 72-73 17.391462175112533 30.618245555809082 33.67732166193034 18.312970607148042 74-75 17.479685336624474 31.642648606883387 32.92245943549349 17.955206620998645 76-77 17.79145557969624 31.158770242311423 33.43150929992585 17.618264878066487 78-79 17.247047963782133 30.496336208014192 33.25208859479446 19.00452723340922 80-81 17.70986191555226 32.25980409949547 31.94868630511322 18.08164767983905 82-83 17.24069983302739 31.926511309542327 32.00584922684422 18.826939630586065 84-85 16.969489556758553 30.537406923443882 32.10855993196856 20.38454358782901 86-87 17.36494965103135 31.4963651259502 32.04600371265309 19.092681510365352 88-89 18.124873607085263 29.75509590305469 32.56296311660297 19.557067373257077 90-91 17.25761456853371 29.43262779097141 33.113132214006455 20.19662542648843 92-93 17.650749534619635 30.160796046729892 32.39565886970905 19.79279554894142 94-95 17.276800066371454 29.895153846951576 32.74549659327782 20.082549493399153 96-97 17.70095512667614 30.031526439690126 31.509328300164892 20.758190133468844 98-99 17.437724265239666 30.241843486196668 31.349428577354654 20.97100367120901 100-101 17.09204044594244 30.89758879958517 31.71532278973295 20.295047964739435 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1793.0 1 1147.0 2 443.5 3 370.0 4 297.5 5 262.5 6 321.5 7 492.5 8 679.0 9 744.5 10 661.0 11 515.5 12 426.5 13 380.5 14 377.5 15 377.0 16 370.5 17 364.5 18 369.0 19 406.0 20 444.5 21 457.0 22 491.0 23 543.0 24 587.0 25 693.5 26 891.0 27 1077.5 28 1135.5 29 1349.0 30 1789.0 31 2047.5 32 2571.5 33 3393.0 34 4106.0 35 4653.0 36 5353.5 37 5995.5 38 5921.5 39 5337.0 40 4739.0 41 4083.0 42 3315.0 43 3244.0 44 3243.5 45 2795.0 46 2341.0 47 2216.5 48 3703.0 49 3440.5 50 1505.0 51 834.5 52 398.5 53 251.0 54 190.0 55 182.5 56 151.5 57 122.5 58 131.5 59 156.5 60 150.5 61 125.0 62 98.0 63 73.5 64 57.0 65 41.5 66 26.5 67 16.5 68 11.0 69 7.0 70 5.5 71 8.0 72 9.0 73 6.0 74 2.5 75 3.0 76 1.5 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025926348429381813 2 0.005185269685876362 3 0.00829643149740218 4 0.0 5 0.005185269685876362 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.00414821574870109 20-21 5.185269685876363E-4 22-23 0.0 24-25 0.00414821574870109 26-27 0.005185269685876362 28-29 0.006740850591639271 30-31 0.025407821460794174 32-33 5.185269685876363E-4 34-35 0.0046667427172887264 36-37 0.013481701183278542 38-39 0.0062223236230516355 40-41 0.012444647246103271 42-43 0.007777904528814544 44-45 0.0031111618115258178 46-47 0.00414821574870109 48-49 0.0015555809057629089 50-51 0.0 52-53 0.0046667427172887264 54-55 0.0015555809057629089 56-57 0.0 58-59 0.0015555809057629089 60-61 0.0 62-63 0.0 64-65 0.00414821574870109 66-67 0.002074107874350545 68-69 0.0015555809057629089 70-71 0.0031111618115258178 72-73 0.009333485434577453 74-75 0.006740850591639271 76-77 0.0015555809057629089 78-79 0.010889066340340361 80-81 5.185269685876363E-4 82-83 0.00414821574870109 84-85 0.0010370539371752725 86-87 0.0 88-89 0.0015555809057629089 90-91 0.0 92-93 5.185269685876363E-4 94-95 0.0 96-97 0.0 98-99 0.0010370539371752725 100-101 0.002074107874350545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 96427.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.451294761840565 #Duplication Level Percentage of deduplicated Percentage of total 1 68.27353075812529 20.107438787891358 2 10.996866086834043 6.477438891596752 3 5.063558576006198 4.473850684974125 4 3.207859431670129 3.779024547066693 5 1.989506672770168 2.929677372520145 6 1.4296278037959083 2.526263390958964 7 1.2676502693756821 2.6133759216816865 8 0.8556639318285856 2.01603285386873 9 0.7253776541427515 1.9226979995229554 >10 5.722032465931899 32.028373795721116 >50 0.33804007183351525 6.840407769608098 >100 0.10563752244797352 5.097120101216465 >500 0.010563752244797352 2.014995799931554 >1k 0.014085002993063136 7.17330208344136 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2123 2.2016655086231034 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2083 2.1601833511360926 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1385 1.4363197029877524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1326 1.3751335206944113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 819 0.8493471745465482 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 564 0.5848984205668537 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 560 0.5807502048181525 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 343 0.35570950045111843 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.32044966658715923 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 287 0.2976344799693032 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 230 0.23852240555031268 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 224 0.23230008192726104 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 188 0.19496614018895123 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 183 0.18978087050307485 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 181 0.18770676262872432 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 179 0.18563265475437377 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 173 0.17941033113132213 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 161 0.16696568388521887 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 160 0.16592862994804358 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 156 0.1617804141993425 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 153 0.1586692523878167 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.15555809057629086 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 148 0.15348398270194033 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 145 0.1503728208904145 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 133 0.13792817364431123 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 132 0.13689111970713597 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 128 0.13274290395843488 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 127 0.1317058500212596 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 127 0.1317058500212596 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 123 0.1275576342725585 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.12133531064950688 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 117 0.12133531064950688 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 114 0.11822414883798106 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 109 0.1130388791521047 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 108 0.11200182521492942 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 107 0.11096477127775414 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 103 0.10681655552905306 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 98 0.1016312858431767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 98 0.1016312858431767 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 98 0.1016312858431767 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.185269685876363E-4 0.0 12-13 0.0 0.0 0.0 0.0010370539371752725 0.0 14-15 0.0 0.0 0.0 0.0010370539371752725 0.0 16-17 0.0 0.0 0.0 0.0010370539371752725 0.0 18-19 0.0 0.0 0.0 0.0010370539371752725 0.0 20-21 0.0 0.0 0.0 0.002592634842938181 0.0 22-23 0.0 0.0 0.0 0.009852012403165088 0.0 24-25 0.0 0.0 0.0 0.01762991693197963 0.0 26-27 0.0 0.0 0.0 0.02644487539796945 0.0 28-29 0.0 0.0 0.0 0.04044510354983563 0.0 30-31 0.0 0.0 0.0 0.06533439804204216 0.0 32-33 0.0 0.0 0.0 0.09385338131436216 0.0 34-35 0.0 0.0 0.0 0.12237236458668216 0.0 36-37 0.0 0.0 0.0 0.15918777935640432 0.0 38-39 0.0 0.0 0.0 0.2203739616497454 0.0 40-41 0.0 0.0 0.0 0.28207867091167416 0.0 42-43 0.0 0.0 0.0 0.3313387329274996 0.0 44-45 0.0 0.0 0.0 0.3702282555715723 0.0 46-47 0.0 0.0 0.0 0.4345255996764392 0.0 48-49 0.0 0.0 0.0 0.46667427172887266 0.0 50-51 0.0 0.0 0.0 0.4936376740954297 0.0 52-53 0.0 0.0 0.0 0.5470459518599562 0.0 54-55 0.0 0.0 0.0 0.6637145197921744 0.0 56-57 0.0 0.0 0.0 0.81823555643129 0.0 58-59 0.0 0.0 0.0 1.0033496842170762 0.0 60-61 0.0 0.0 0.0 1.238760927955863 0.0 62-63 0.0 0.0 0.0 1.3554294958880813 0.0 64-65 0.0 0.0 0.0 1.4990614661868564 0.0 66-67 0.0 0.0 0.0 1.7759548674126542 0.0 68-69 0.0 0.0 0.0 2.1809244298795982 0.0 70-71 0.0 0.0 0.0 2.5718937641946757 0.0 72-73 0.0 0.0 0.0 3.0784946125047963 0.0 74-75 0.0 0.0 0.0 3.5353168718305037 0.0 76-77 0.0 0.0 0.0 3.867174131726591 0.0 78-79 0.0 0.0 0.0 4.1549565992927295 0.0 80-81 0.0 0.0 0.0 4.353033901293206 0.0 82-83 0.0 0.0 0.0 4.652223962168272 0.0 84-85 0.0 0.0 0.0 5.4004583778402315 0.0 86-87 0.0 0.0 0.0 6.509587563649186 0.0 88-89 0.0 0.0 0.0 7.565308471693613 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTATCG 15 6.6144497E-4 94.94553 9 TTTTACG 15 6.6144497E-4 94.94553 2 TATGTAT 20 0.0020730093 71.20915 7 GACATAT 45 2.0359221E-7 63.297028 1 ATGTATC 25 0.0050187474 56.967323 8 CATATAG 70 4.286014E-6 40.690945 3 ACATATA 70 4.286014E-6 40.690945 2 AGTGAAT 25 0.0016047013 38.037403 28-29 GGATATG 75 6.8836143E-6 37.978218 9 ATTGTAG 25 0.0016170307 37.978214 58-59 CACCAAG 65 1.3236354E-4 36.517517 6 TTGATTG 55 0.0025751994 34.52565 5 TAGATGG 55 0.0025751994 34.52565 5 TATCACC 70 2.04634E-4 33.909122 3 ATCACCA 70 2.04634E-4 33.909122 4 CATGGGG 115 1.0248732E-7 33.024536 6 TGTGATC 30 0.0039489367 31.656723 44-45 CTATCAC 75 3.0680269E-4 31.648514 2 ACCAAGC 75 3.0680269E-4 31.648514 7 TGTAGGC 30 0.003953956 31.648512 60-61 >>END_MODULE