FastQCFastQC Report
Thu 26 May 2016
SRR936949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98557
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18461.8730277910244835No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13281.347443611311221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13151.3342532747547104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11361.1526324867842974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.7021317613157868No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5380.5458770051848169No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4580.4647057032985988No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4350.44136895400631104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.2556896009415871No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2480.2516310358472762No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.2414846231114989No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT2120.21510394999847807No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC2000.20292825471554532No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2000.20292825471554532No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA1960.1988696896212344No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1630.16538652759316944No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG1480.15016690848950354No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.14813762594234808No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG1410.14306441957445945No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG1320.1339326481122599No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1290.13088872429152673No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT1260.12784480047079355No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT1250.12683015919721582No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.12175695282932719No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.11668374646143856No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC1120.11363982264070538No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG1100.11161054009354993No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.11059589881997219No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA1080.10958125754639447No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA1070.10856661627281675No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA1060.10755197499923903No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG1040.10552269245208355No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA1040.10552269245208355No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT1040.10552269245208355No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT1000.10146412735777266No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT990.10044948608419492No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA990.10044948608419492No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACACA156.591121E-495.0314948
ATGGGAC250.004981029357.0768665
GGGTATG250.005001120757.0188947
TTAGCAT405.328515E-447.5640563
ATGGGTA500.001598024538.0512475
CATGGGG1054.5298293E-836.2392854
CATGGGT550.00255130234.592044
GCATTAT550.002557675634.5744676
ATGGGAT1509.1858965E-1031.7093725
GTCCCAC804.442278E-429.7124319
ATAGGTG650.005780270329.2701914
TAGGTGG650.005780270329.2701915
TATAGGT650.005780270329.2701913
TTGAAGG650.005794659329.2553169
GGGATGA650.00580907629.240467
GTACATG7300.028.6687471
TTATAGG856.3153246E-427.9788552
ACATGGG7350.027.8265933
TACATGG7800.027.4408022
ATCTAAC700.00830021827.1794622
Produced by FastQC (version 0.11.5)