##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936949_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98557 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06747364469292 33.0 31.0 34.0 31.0 34.0 2 32.221303408180034 33.0 31.0 34.0 31.0 34.0 3 32.18277747902229 34.0 31.0 34.0 30.0 34.0 4 35.765293180596004 37.0 35.0 37.0 35.0 37.0 5 35.767586269874286 37.0 35.0 37.0 35.0 37.0 6 35.90473533082379 37.0 36.0 37.0 35.0 37.0 7 35.871292754446664 37.0 36.0 37.0 35.0 37.0 8 35.85807197865195 37.0 36.0 37.0 35.0 37.0 9 37.48996012459795 39.0 37.0 39.0 35.0 39.0 10-11 37.41038180951125 39.0 37.0 39.0 35.0 39.0 12-13 37.366097791125945 39.0 37.0 39.0 35.0 39.0 14-15 38.54473046054567 40.0 38.0 41.0 34.5 41.0 16-17 38.44750753371146 40.0 38.0 41.0 34.0 41.0 18-19 38.41278650932963 40.0 38.0 41.0 34.0 41.0 20-21 38.33142242560143 40.0 38.0 41.0 34.0 41.0 22-23 38.24377263918342 40.0 38.0 41.0 34.0 41.0 24-25 38.30692391205089 40.0 38.0 41.0 34.0 41.0 26-27 38.21319135119778 40.0 38.0 41.0 34.0 41.0 28-29 38.09440222409367 40.0 38.0 41.0 34.0 41.0 30-31 37.8725356900068 40.0 37.0 41.0 34.0 41.0 32-33 37.599977677891985 40.0 36.0 41.0 33.5 41.0 34-35 37.384366407256714 40.0 35.0 41.0 33.0 41.0 36-37 37.144155158943555 39.0 35.0 41.0 33.0 41.0 38-39 36.96294022748258 39.0 35.0 41.0 33.0 41.0 40-41 36.78387126231521 39.0 35.0 41.0 32.5 41.0 42-43 36.511891595726325 39.0 35.0 41.0 31.5 41.0 44-45 36.15293687916637 39.0 35.0 41.0 30.5 41.0 46-47 35.66532564911675 39.0 35.0 41.0 29.5 41.0 48-49 35.22507280051138 39.0 35.0 40.5 26.5 41.0 50-51 34.4429822336313 38.0 34.0 40.0 25.0 40.5 52-53 34.61391377578457 38.5 35.0 40.0 24.5 40.5 54-55 35.01385999979707 39.0 35.0 40.5 25.5 41.0 56-57 34.922334283713994 39.0 35.0 41.0 23.5 41.0 58-59 34.70799638787706 39.0 35.0 41.0 22.5 41.0 60-61 34.44779163326806 39.0 35.0 41.0 22.0 41.0 62-63 34.10017553294033 38.0 35.0 40.0 18.5 41.0 64-65 33.72005539941354 38.0 34.5 40.0 11.0 41.0 66-67 33.33131081506133 37.0 34.0 40.0 6.5 41.0 68-69 32.918788112462835 37.0 34.0 39.5 2.0 41.0 70-71 32.45995210893189 36.0 33.5 39.0 2.0 41.0 72-73 31.97136175005327 36.0 33.0 39.0 2.0 40.5 74-75 31.46864251144008 35.0 32.5 38.0 2.0 40.0 76-77 30.98039205738811 35.0 32.0 37.0 2.0 39.5 78-79 30.52273303773451 35.0 31.5 37.0 2.0 39.0 80-81 30.01657416520389 35.0 31.0 36.0 2.0 39.0 82-83 29.539124567509155 35.0 30.5 36.0 2.0 37.5 84-85 29.09867386385543 35.0 30.0 35.5 2.0 37.0 86-87 28.691533833213267 34.0 29.5 35.0 2.0 36.5 88-89 28.242017309780127 34.0 29.0 35.0 2.0 36.0 90-91 27.797543553476668 34.0 29.0 35.0 2.0 36.0 92-93 27.44930344876569 34.0 27.5 35.0 2.0 35.5 94-95 27.096761265054738 34.0 26.5 35.0 2.0 35.0 96-97 26.630508233813934 34.0 25.5 35.0 2.0 35.0 98-99 26.087649786418012 34.0 24.5 35.0 2.0 35.0 100-101 23.82176304067697 31.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05833751055065761 1101 2 0.32067314461340857 1101 3 0.12078550156958556 1101 4 0.12834126275793523 1101 5 0.17137076191569633 1101 6 0.06048791952645871 1101 7 -0.5085544978585403 1101 8 -0.0646913877295816 1101 9 0.07403902846471055 1101 10-11 0.4257569240178256 1101 12-13 0.6179730653960007 1101 14-15 0.19701563622898988 1101 16-17 0.49271874868859555 1101 18-19 -0.18383917687654616 1101 20-21 0.29803774697165153 1101 22-23 0.23796685988681787 1101 24-25 0.5415084777816404 1101 26-27 0.6416433376879169 1101 28-29 0.2693414895457025 1101 30-31 0.5735879327542861 1101 32-33 0.8491970121204062 1101 34-35 0.7750234873399293 1101 36-37 0.7510132625233297 1101 38-39 0.9125705999016134 1101 40-41 0.8812045762533245 1101 42-43 0.942827784862331 1101 44-45 1.1030374420037035 1101 46-47 1.3744100347990766 1101 48-49 1.610500343031191 1101 50-51 1.6422429979896407 1101 52-53 1.9878502685523642 1101 54-55 2.2814212692702824 1101 56-57 2.399182580698117 1101 58-59 2.2271363008306437 1101 60-61 2.2617156439709376 1101 62-63 2.6522544656678804 1101 64-65 2.747046992662817 1101 66-67 2.2260231634948724 1101 68-69 2.289631537956275 1101 70-71 2.419525948966914 1101 72-73 2.032228993199446 1101 74-75 2.014452222931155 1101 76-77 2.64913754165136 1101 78-79 2.4151675528675867 1101 80-81 2.1953022287770096 1101 82-83 1.9875574840457766 1101 84-85 1.7874118332100188 1101 86-87 2.309725114533368 1101 88-89 1.4576285936922524 1101 90-91 2.049370012631311 1101 92-93 2.349191141619496 1101 94-95 2.615187188817746 1101 96-97 2.208042271240714 1101 98-99 2.5018280361423457 1101 100-101 2.9329118856281333 1102 1 0.490221568521676 1102 2 0.6456731446134043 1102 3 0.7059304291058197 1102 4 0.6703702482651792 1102 5 0.4641243851041068 1102 6 0.5199082093815264 1102 7 0.4620976760544977 1102 8 0.5349462934298401 1102 9 0.8019375791893495 1102 10-11 0.709090257351157 1102 12-13 0.3857266885844055 1102 14-15 0.3495518681130534 1102 16-17 0.3376462849204742 1102 18-19 0.48536372167418307 1102 20-21 0.5186899208846896 1102 22-23 0.7352494685824738 1102 24-25 0.6853490574917842 1102 26-27 0.6646505840647308 1102 28-29 0.03039221418337945 1102 30-31 0.3165227153629786 1102 32-33 1.1501028092218561 1102 34-35 0.9087191395138419 1102 36-37 0.6001074654218854 1102 38-39 0.8734401651190069 1102 40-41 1.1585596487170946 1102 42-43 1.3361248863116089 1102 44-45 1.0709722246123974 1102 46-47 1.1186129333498016 1102 48-49 1.1548844010022066 1102 50-51 0.9641632878447126 1102 52-53 0.9639372250740976 1102 54-55 0.8062401098499947 1102 56-57 0.613494174901021 1102 58-59 0.5144551414103589 1102 60-61 0.349577962811523 1102 62-63 0.9281602627693317 1102 64-65 1.0330977173004925 1102 66-67 0.40863185914704303 1102 68-69 0.8115518278113427 1102 70-71 1.0568447895466235 1102 72-73 1.1316855149385816 1102 74-75 1.3834739620615935 1102 76-77 1.5502244981730975 1102 78-79 1.5912545093893264 1102 80-81 1.777910924429186 1102 82-83 2.037195165205194 1102 84-85 1.7783538621955266 1102 86-87 1.2439642449681507 1102 88-89 1.0797300429676149 1102 90-91 0.8433917517617466 1102 92-93 1.1500969387209459 1102 94-95 1.693991536643832 1102 96-97 1.6723538654436112 1102 98-99 0.8029149926640855 1102 100-101 1.7169698566426277 1103 1 0.28397853619168245 1103 2 0.4012287001689643 1103 3 0.33253763832172467 1103 4 0.10782844224511479 1103 5 0.4517126422575828 1103 6 0.5724537314922671 1103 7 0.5767019123978656 1103 8 0.49406929603110683 1103 9 0.3614321908578759 1103 10-11 0.40396205222295833 1103 12-13 0.5057935782165117 1103 14-15 0.31774213195549095 1103 16-17 0.4397272956971321 1103 18-19 0.5561180880807228 1103 20-21 0.419618943552841 1103 22-23 0.6466207060406575 1103 24-25 0.8699272812004395 1103 26-27 0.9064937650383413 1103 28-29 1.268593626297843 1103 30-31 0.9040366507029987 1103 32-33 0.9343465847699832 1103 34-35 0.7384850258014595 1103 36-37 1.0073166813267491 1103 38-39 1.044194531525548 1103 40-41 1.1610122685610165 1103 42-43 1.1397294942640457 1103 44-45 1.5191699206361804 1103 46-47 0.9167177271067715 1103 48-49 1.472572992603837 1103 50-51 1.1073071005537471 1103 52-53 1.253234883936976 1103 54-55 1.5182802436292562 1103 56-57 1.821297965313505 1103 58-59 1.98888843758278 1103 60-61 1.766523336278631 1103 62-63 2.110694636608045 1103 64-65 1.9807008388166594 1103 66-67 2.1679035053752145 1103 68-69 2.0974306832554177 1103 70-71 2.889397743838707 1103 72-73 2.5652418137122623 1103 74-75 2.641482137461068 1103 76-77 2.266872584386409 1103 78-79 1.870295757995791 1103 80-81 1.7978663313411118 1103 82-83 1.4493096207979121 1103 84-85 1.4290784998766881 1103 86-87 1.5393191316273835 1103 88-89 1.3296371407008003 1103 90-91 1.7885794143407097 1103 92-93 1.574511654440009 1103 94-95 2.3915760777066346 1103 96-97 2.0741747498731904 1103 98-99 1.844349403663717 1103 100-101 2.073082825799073 1104 1 0.31438470819077935 1104 2 0.3146996932859736 1104 3 0.4642220797406793 1104 4 0.19087813591427505 1104 5 0.3796303489363453 1104 6 0.24426373073589502 1104 7 0.26379063488482046 1104 8 0.010898582771893928 1104 9 -0.13134445236124748 1104 10-11 0.15333804496177805 1104 12-13 0.07556893560248312 1104 14-15 0.41788584271866824 1104 16-17 0.6788544419039297 1104 18-19 0.38555610335944834 1104 20-21 0.2584359770601452 1104 22-23 0.4110863289133633 1104 24-25 0.23759992320936618 1104 26-27 0.12663596305664981 1104 28-29 -0.07907296473157999 1104 30-31 0.3144065168250805 1104 32-33 0.38234597967203143 1104 34-35 0.2619703899947936 1104 36-37 0.3643244719628598 1104 38-39 0.3470838742378959 1104 40-41 0.9170821573742671 1104 42-43 1.141242239139622 1104 44-45 0.8369976189948503 1104 46-47 1.5488938106102879 1104 48-49 1.5427643548305952 1104 50-51 1.6035630569866939 1104 52-53 1.4564343393488244 1104 54-55 1.6619153695652713 1104 56-57 1.831962816686321 1104 58-59 1.527652525019441 1104 60-61 1.882475230991595 1104 62-63 1.6329698049009167 1104 64-65 1.1183228038722532 1104 66-67 1.5693859953532794 1104 68-69 1.2013940748294232 1104 70-71 1.6019005802353519 1104 72-73 1.9836670462967874 1104 74-75 1.470315054789566 1104 76-77 0.8480313469610969 1104 78-79 1.5412737475578524 1104 80-81 1.7174261225823209 1104 82-83 1.8273067465826465 1104 84-85 1.631143396631849 1104 86-87 2.0531985658608 1104 88-89 1.5068173842527237 1104 90-91 1.4311178002419283 1104 92-93 1.2239330295250994 1104 94-95 0.98941285253456 1104 96-97 1.7474596753705072 1104 98-99 1.3971820184432318 1104 100-101 1.3438263399054229 1105 1 0.4152002556486991 1105 2 0.6888103995153685 1105 3 -0.07872430235197925 1105 4 0.28128243922852647 1105 5 0.571370761915702 1105 6 0.10362517442841579 1105 7 -0.03943685079971715 1105 8 -0.08184825047467825 1105 9 -0.15390214800587643 1105 10-11 0.4277177083315493 1105 12-13 0.2821887516705104 1105 14-15 0.04750583230742933 1105 16-17 0.31918933692388407 1105 18-19 0.35978827410384895 1105 20-21 0.04166519795203527 1105 22-23 0.33478058537701116 1105 24-25 -0.2129032869242451 1105 26-27 -1.0649743093709034 1105 28-29 -0.4612957653562546 1105 30-31 -0.3237159888143424 1105 32-33 -0.4961461251344943 1105 34-35 -0.8195843557973248 1105 36-37 -0.3183494825747033 1105 38-39 -0.8580176353924998 1105 40-41 -0.19796209041334123 1105 42-43 -0.3245741759219811 1105 44-45 -0.6604429501531612 1105 46-47 -1.4128448671617022 1105 48-49 -1.4417055393217524 1105 50-51 -1.1668256294613357 1105 52-53 -1.4601889471339078 1105 54-55 -1.5345101032787412 1105 56-57 -1.2201801643999133 1105 58-59 -1.2017852677968008 1105 60-61 -1.1806863168133717 1105 62-63 -0.9867161225674153 1105 64-65 -1.6056490857685581 1105 66-67 -1.2916238953286552 1105 68-69 -0.9890449326319626 1105 70-71 -0.792728952993869 1105 72-73 -1.0935063009182002 1105 74-75 -1.7681458162845267 1105 76-77 -1.8052742230545213 1105 78-79 -1.9083618588971198 1105 80-81 -2.1870507123994614 1105 82-83 -2.0501876139934403 1105 84-85 -1.9875881667899797 1105 86-87 -2.4630690031136915 1105 88-89 -2.0654106219940225 1105 90-91 -1.8729339089373163 1105 92-93 -1.6981127799491347 1105 94-95 -1.3894696739273513 1105 96-97 -1.33092831699458 1105 98-99 -1.3908190226811818 1105 100-101 -1.2935587026071609 1106 1 -0.2833291561160074 1106 2 0.033173144613407146 1106 3 0.3312021682362527 1106 4 0.17834126275793238 1106 5 0.2797040952490306 1106 6 0.44798791952645445 1106 7 0.3956121688081211 1106 8 0.5811419456037541 1106 9 0.08445569513138196 1106 10-11 0.12992359068449133 1106 12-13 0.2638063987293364 1106 14-15 0.3636823028956613 1106 16-17 -0.28957291797807017 1106 18-19 0.5203274897901196 1106 20-21 0.1813710803049844 1106 22-23 0.34734185988681787 1106 24-25 0.26442514444830323 1106 26-27 0.7083100043545869 1106 28-29 0.6110081562123639 1106 30-31 0.48296293275428326 1106 32-33 0.44503034545373765 1106 34-35 0.7416901540065908 1106 36-37 0.6458049291899997 1106 38-39 0.5865289332349519 1106 40-41 -0.005253757080012633 1106 42-43 0.11261945152899955 1106 44-45 0.7332457753370321 1106 46-47 0.9952433681324138 1106 48-49 0.5938336763645253 1106 50-51 0.42140966465630214 1106 52-53 0.2524336018856914 1106 54-55 0.6324629359369496 1106 56-57 0.8585575806981183 1106 58-59 0.6750529674973151 1106 60-61 0.600257310637609 1106 62-63 0.12725446566787468 1106 64-65 -0.03211967400385163 1106 66-67 0.09164816349487381 1106 68-69 0.09275653795627647 1106 70-71 0.08410928230025405 1106 72-73 0.5155623265327733 1106 74-75 0.060285556264496165 1106 76-77 -0.24461245834863732 1106 78-79 -0.37129078046574904 1106 80-81 -0.9713644378896547 1106 82-83 -0.09369251595422412 1106 84-85 -0.42508816678997974 1106 86-87 -0.33194155213330134 1106 88-89 -0.5559130729744126 1106 90-91 -0.32667165403535847 1106 92-93 -0.8133088583805019 1106 94-95 -0.20043781118225468 1106 96-97 0.13720893790737776 1106 98-99 -0.3460886305243207 1106 100-101 -0.4243797810385317 1107 1 0.4805597327728819 1107 2 0.6638549627952273 1107 3 0.48871479449887545 1107 4 0.3525836870003616 1107 5 0.571370761915702 1107 6 0.6190737781123161 1107 7 0.2775566132525711 1107 8 0.537581339543145 1107 9 0.7444935739192573 1107 10-11 0.36161550987641533 1107 12-13 0.22024579266872735 1107 14-15 0.6452479594613152 1107 16-17 0.256355112324961 1107 18-19 -0.13813210616947202 1107 20-21 0.2179872419211435 1107 22-23 0.31056786998782826 1107 24-25 0.08165999293314741 1107 26-27 0.28722414576872524 1107 28-29 0.3439627016669107 1107 30-31 0.08002732669367418 1107 32-33 -0.0978989474755565 1107 34-35 0.12931641663285376 1107 36-37 0.5380082120182834 1107 38-39 0.501459488790502 1107 40-41 0.4687045762533302 1107 42-43 0.19800455253910343 1107 44-45 0.44962835109460997 1107 46-47 1.0324908428798878 1107 48-49 1.176535696566539 1107 50-51 1.3633288565754995 1107 52-53 1.1782543089564044 1107 54-55 1.5590727844217938 1107 56-57 1.876076520092063 1107 58-59 2.1652676139619658 1107 60-61 2.4176499874052837 1107 62-63 2.8993504252638402 1107 64-65 2.351718709834529 1107 66-67 2.4775383150100296 1107 68-69 1.886474972299709 1107 70-71 1.3824047368457073 1107 72-73 0.9214966699671194 1107 74-75 1.267356263335202 1107 76-77 0.6837335012473211 1107 78-79 1.0742584619584932 1107 80-81 1.2407567742315564 1107 82-83 1.3782140497023398 1107 84-85 1.6866542574524424 1107 86-87 1.5828311751394288 1107 88-89 1.4795982906619507 1107 90-91 1.1242437500050464 1107 92-93 0.5970446769730309 1107 94-95 0.7198589059894616 1107 96-97 0.7975624732609141 1107 98-99 1.3202623795766897 1107 100-101 1.8601846129008628 1108 1 -0.4792511419316128 1108 2 -0.18624174900361368 1108 3 -0.4035052785722577 1108 4 -0.014921148589586153 1108 5 0.17420764134832467 1108 6 -0.2253276833104252 1108 7 -0.5250438595606681 1108 8 -0.29252826716220426 1108 9 -0.2335850850104677 1108 10-11 -0.31112250860628166 1108 12-13 -0.34567941687350157 1108 14-15 -0.3570623779554083 1108 16-17 -0.05763586124048459 1108 18-19 -0.23720797120277837 1108 20-21 -0.18653672111345543 1108 22-23 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-1.3185621460410601 2316 74-75 -1.0515287897270653 2316 76-77 -1.5037105596144578 2316 78-79 -2.765212193967855 2316 80-81 -3.0262167585647646 2316 82-83 -3.0123370307221577 2316 84-85 -2.9675459726971525 2316 86-87 -3.528407796859039 2316 88-89 -3.0127300560967747 2316 90-91 -3.423256569647169 2316 92-93 -4.341262444878396 2316 94-95 -3.6935153428278227 2316 96-97 -3.881250977704436 2316 98-99 -3.5105137360095533 2316 100-101 -2.5611809413760867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 323.0 3 62.0 4 66.0 5 50.0 6 62.0 7 82.0 8 87.0 9 144.0 10 119.0 11 179.0 12 315.0 13 465.0 14 528.0 15 782.0 16 1054.0 17 1568.0 18 1421.0 19 692.0 20 661.0 21 664.0 22 727.0 23 814.0 24 872.0 25 927.0 26 1039.0 27 1122.0 28 1334.0 29 1536.0 30 1832.0 31 2132.0 32 2726.0 33 3661.0 34 5268.0 35 8389.0 36 11691.0 37 19287.0 38 21622.0 39 4254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.167202898697816 25.028672343621544 17.180062317943303 19.62406243973733 2 13.518834226335445 24.87010878389349 41.0232992368891 20.58775775288196 3 15.59404935866212 31.84059912323429 33.63675109595714 18.928600422146452 4 9.797434440204595 21.706787367053664 42.19676057481529 26.299017617926445 5 9.345462298344112 40.81748682700996 37.402154380336455 12.434896494309472 6 21.635723204044137 43.539431344086566 20.361779663597698 14.463065788271598 7 20.64237872944997 38.3509234828496 25.857519788918204 15.149177998782221 8 20.930492249281777 36.00251758758261 23.849066563797496 19.217923599338118 9 23.640700329865517 19.299670134483634 25.043390002537425 32.01623953311342 10-11 19.731046381812646 30.158834872627626 30.423221353902363 19.68689739165736 12-13 22.46386373786999 30.074810183117464 29.99766535385115 17.463660725161397 14-15 20.532687897054945 30.913962126286304 29.898617792120806 18.654732184537945 16-17 15.550414028251339 33.95031660983926 31.655910050332846 18.843359311576556 18-19 15.37173476222371 32.40374271854513 35.39497452759341 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32.55493042062167 33.77808668124375 18.098755536164536 46-47 16.411315279482938 32.511135687977514 32.7531276317258 18.324421400813744 48-49 16.550321133963088 32.48120377040698 32.74551782217397 18.222957273455968 50-51 16.669541483608473 32.22247024564465 33.02302221049748 18.0849660602494 52-53 16.149030510263096 33.06868106780848 32.92815325141796 17.854135170510467 54-55 16.259354151340656 32.46391517211639 32.92255396869689 18.354176707846072 56-57 16.77523438451236 33.40283696578595 31.56885425544868 18.253074394253012 58-59 16.286172328422417 31.926518933398274 32.93558991842202 18.851718819757295 60-61 16.615002156211155 32.52834783491033 32.559295806803476 18.297354202075038 62-63 17.29526244761702 32.313070124704474 32.7113329883413 17.68033443933721 64-65 17.288403878057316 31.752989462683086 33.189250617678546 17.769356041581055 66-67 16.387936108461368 32.05841164173652 32.570376083294434 18.983276166507682 68-69 15.925696657262892 32.34103225020804 33.26449635688336 18.468774735645717 70-71 16.869487551635594 31.927796441584537 32.72250246125428 18.480213545525594 72-73 16.75766186320276 31.118834990866652 32.39090724578851 19.732595900142076 74-75 16.56974679048054 32.12259603186685 32.576241944486725 18.73141523316588 76-77 16.56026065244311 31.15649296603804 33.41233430096831 18.870912080550536 78-79 16.23526546270126 30.8756742493822 33.45799229719238 19.431067990724156 80-81 16.187517765054615 32.407520201404964 32.35371746457141 19.051244568969018 82-83 16.391761995148144 31.52285345973873 32.35112008851084 19.73426445660228 84-85 16.128033774286685 30.78250191553384 31.816636474808828 21.27282783537065 86-87 16.561889540046174 31.26163837937946 32.378415404520894 19.79805667605348 88-89 17.443058141835838 29.548372038744308 32.804801940259885 20.203767879159965 90-91 16.483399383978366 29.187450208300564 33.62341491137712 20.70573549634396 92-93 16.600692224150706 30.20005480953686 33.16179978279183 20.037453183520597 94-95 16.208345171895928 28.981308600884848 33.546596582376104 21.263749644843124 96-97 16.17615717953662 29.040867897338057 32.6501720166029 22.13280290652242 98-99 16.142521479210966 29.385286042699605 32.538276892784104 21.93391558530533 100-101 16.187218319866822 29.61295627106257 32.87841568882212 21.32140972024849 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1117.0 1 729.0 2 323.0 3 296.0 4 259.5 5 252.0 6 369.0 7 633.5 8 880.5 9 931.0 10 708.5 11 470.0 12 434.0 13 432.0 14 412.0 15 392.5 16 344.5 17 303.5 18 302.0 19 329.0 20 345.0 21 385.5 22 446.0 23 499.5 24 550.0 25 631.5 26 891.0 27 1165.5 28 1236.5 29 1366.5 30 1750.0 31 2123.0 32 2720.0 33 3535.5 34 4320.5 35 4889.0 36 5636.5 37 6455.5 38 6290.0 39 5617.0 40 4876.0 41 4125.0 42 3441.0 43 3295.0 44 3238.0 45 2871.0 46 2443.0 47 2298.0 48 3737.5 49 3398.5 50 1419.0 51 730.0 52 382.5 53 269.5 54 220.5 55 196.0 56 149.5 57 144.0 58 155.5 59 162.5 60 171.5 61 152.0 62 118.0 63 84.5 64 63.5 65 49.5 66 33.5 67 26.5 68 25.0 69 15.5 70 6.5 71 5.0 72 5.0 73 7.5 74 6.0 75 6.5 76 6.0 77 1.5 78 1.5 79 1.0 80 0.0 81 1.5 82 1.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030439238207331798 2 0.013190336556510444 3 0.013190336556510444 4 0.02130746674513226 5 0.060878476414663596 6 0.04464421603741997 7 0.017248901650821352 8 0.05073206367888633 9 0.03246852075448725 10-11 0.027395314386598618 12-13 0.04160029221668679 14-15 0.019278184197976805 16-17 0.013190336556510444 18-19 0.019278184197976805 20-21 0.013190336556510444 22-23 0.008117130188621813 24-25 0.0010146412735777266 26-27 0.0 28-29 0.00304392382073318 30-31 0.0 32-33 5.073206367888633E-4 34-35 5.073206367888633E-4 36-37 0.0 38-39 0.0 40-41 5.073206367888633E-4 42-43 5.073206367888633E-4 44-45 5.073206367888633E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.00456588573109977 56-57 0.0010146412735777266 58-59 0.0010146412735777266 60-61 0.00456588573109977 62-63 0.004058565094310906 64-65 0.0025366031839443165 66-67 0.015219619103665899 68-69 0.015219619103665899 70-71 0.030439238207331798 72-73 0.017248901650821352 74-75 0.022322108018709985 76-77 0.03551244457522043 78-79 0.021814787381921122 80-81 0.0497174224053086 82-83 0.03855636839595361 84-85 0.020800146108343395 86-87 0.014712298466877037 88-89 0.013697657193299309 90-91 0.021814787381921122 92-93 0.034497803301642704 94-95 0.00913177146219954 96-97 0.020292825471554532 98-99 0.03196120011769839 100-101 0.04160029221668679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 98557.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.020627657091836 #Duplication Level Percentage of deduplicated Percentage of total 1 69.23102083537762 21.47589719654616 2 10.519085467569424 6.5261726716519375 3 4.961894482059334 4.617632436052234 4 3.0320871357079775 3.76228984242621 5 1.8970987472606549 2.9424596933754072 6 1.5307624374448043 2.8491126962062565 7 1.2560102050829163 2.727355743376929 8 0.8929447551761359 2.2159765414937547 9 0.8013606777221731 2.237284008238887 >10 5.449252608510777 32.159055166046045 >50 0.3172734111797992 6.713881307263817 >100 0.09158407745396265 4.817516766947046 >500 0.006541719818140189 1.2480087665006037 >1k 0.013083439636280378 5.707357163874712 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1846 1.8730277910244835 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1328 1.347443611311221 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1315 1.3342532747547104 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1136 1.1526324867842974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 692 0.7021317613157868 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 538 0.5458770051848169 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 458 0.4647057032985988 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 435 0.44136895400631104 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 252 0.2556896009415871 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 248 0.2516310358472762 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 238 0.2414846231114989 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 212 0.21510394999847807 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 200 0.20292825471554532 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 200 0.20292825471554532 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 196 0.1988696896212344 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 163 0.16538652759316944 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 148 0.15016690848950354 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.14813762594234808 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 141 0.14306441957445945 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 132 0.1339326481122599 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 129 0.13088872429152673 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 126 0.12784480047079355 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 125 0.12683015919721582 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120 0.12175695282932719 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.11668374646143856 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 112 0.11363982264070538 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 110 0.11161054009354993 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109 0.11059589881997219 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 108 0.10958125754639447 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 107 0.10856661627281675 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 106 0.10755197499923903 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 104 0.10552269245208355 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 104 0.10552269245208355 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 104 0.10552269245208355 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 100 0.10146412735777266 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 99 0.10044948608419492 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 99 0.10044948608419492 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.073206367888633E-4 0.0 18-19 0.0 0.0 0.0 0.0010146412735777266 0.0 20-21 0.0 0.0 0.0 0.002029282547155453 0.0 22-23 0.0 0.0 0.0 0.006595168278255223 0.0 24-25 0.0 0.0 0.0 0.010146412735777266 0.0 26-27 0.0 0.0 0.0 0.016234260377243626 0.0 28-29 0.0 0.0 0.0 0.02587335247623203 0.0 30-31 0.0 0.0 0.0 0.043629574763842244 0.0 32-33 0.0 0.0 0.0 0.07102488915044086 0.0 34-35 0.0 0.0 0.0 0.10197144799456152 0.0 36-37 0.0 0.0 0.0 0.1339326481122599 0.0 38-39 0.0 0.0 0.0 0.19176720070619033 0.0 40-41 0.0 0.0 0.0 0.24351390565865438 0.0 42-43 0.0 0.0 0.0 0.2988118550686405 0.0 44-45 0.0 0.0 0.0 0.3399048266485384 0.0 46-47 0.0 0.0 0.0 0.40027598242641316 0.0 48-49 0.0 0.0 0.0 0.4464421603741997 0.0 50-51 0.0 0.0 0.0 0.47434479539758717 0.0 52-53 0.0 0.0 0.0 0.5397891575433506 0.0 54-55 0.0 0.0 0.0 0.6392240023539677 0.0 56-57 0.0 0.0 0.0 0.7929421553009933 0.0 58-59 0.0 0.0 0.0 0.9887679211014946 0.0 60-61 0.0 0.0 0.0 1.220613452114005 0.0 62-63 0.0 0.0 0.0 1.3596193065941535 0.0 64-65 0.0 0.0 0.0 1.5036983674421909 0.0 66-67 0.0 0.0 0.0 1.781202755765699 0.0 68-69 0.0 0.0 0.0 2.1870592651967895 0.0 70-71 0.0 0.0 0.0 2.5868279269864143 0.0 72-73 0.0 0.0 0.0 3.085016792313078 0.0 74-75 0.0 0.0 0.0 3.5451566098805767 0.0 76-77 0.0 0.0 0.0 3.8911492841705817 0.0 78-79 0.0 0.0 0.0 4.180322047140233 0.0 80-81 0.0 0.0 0.0 4.397962600322655 0.0 82-83 0.0 0.0 0.0 4.689164645839464 0.0 84-85 0.0 0.0 0.0 5.404486743711761 0.0 86-87 0.0 0.0 0.0 6.411010887100866 0.0 88-89 0.0 0.0 0.0 7.478413506904634 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACACA 15 6.591121E-4 95.031494 8 ATGGGAC 25 0.0049810293 57.076866 5 GGGTATG 25 0.0050011207 57.018894 7 TTAGCAT 40 5.328515E-4 47.564056 3 ATGGGTA 50 0.0015980245 38.051247 5 CATGGGG 105 4.5298293E-8 36.239285 4 CATGGGT 55 0.002551302 34.59204 4 GCATTAT 55 0.0025576756 34.574467 6 ATGGGAT 150 9.1858965E-10 31.709372 5 GTCCCAC 80 4.442278E-4 29.712431 9 ATAGGTG 65 0.0057802703 29.270191 4 TAGGTGG 65 0.0057802703 29.270191 5 TATAGGT 65 0.0057802703 29.270191 3 TTGAAGG 65 0.0057946593 29.255316 9 GGGATGA 65 0.005809076 29.24046 7 GTACATG 730 0.0 28.668747 1 TTATAGG 85 6.3153246E-4 27.978855 2 ACATGGG 735 0.0 27.826593 3 TACATGG 780 0.0 27.440802 2 ATCTAAC 70 0.008300218 27.179462 2 >>END_MODULE