##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936949_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98557 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.350862952403176 33.0 31.0 34.0 31.0 34.0 2 32.48308085676309 34.0 31.0 34.0 31.0 34.0 3 32.53621762026036 34.0 31.0 34.0 31.0 34.0 4 36.182381768925595 37.0 35.0 37.0 35.0 37.0 5 36.11411670403929 37.0 35.0 37.0 35.0 37.0 6 36.19944803514717 37.0 36.0 37.0 35.0 37.0 7 36.15503718660268 37.0 36.0 37.0 35.0 37.0 8 36.13827531276317 37.0 36.0 37.0 35.0 37.0 9 37.83548606390211 39.0 38.0 39.0 35.0 39.0 10-11 37.77623608673154 39.0 37.0 39.0 35.0 39.0 12-13 37.71616425012937 39.0 37.0 39.0 35.0 39.0 14-15 38.871485536288645 40.0 38.0 41.0 35.5 41.0 16-17 38.75343202410788 40.0 38.0 41.0 35.5 41.0 18-19 38.85237476790081 40.0 38.0 41.0 35.0 41.0 20-21 38.911300059863834 40.0 38.0 41.0 35.0 41.0 22-23 38.81755227939162 40.0 38.0 41.0 35.0 41.0 24-25 38.70156863540895 40.0 38.0 41.0 35.0 41.0 26-27 38.473254056028495 40.0 38.0 41.0 34.5 41.0 28-29 38.33568391895045 40.0 37.0 41.0 34.0 41.0 30-31 38.184690077822985 40.0 37.0 41.0 34.0 41.0 32-33 37.89531945980499 40.0 36.0 41.0 34.0 41.0 34-35 37.677912274115485 40.0 35.0 41.0 33.0 41.0 36-37 37.439182402061746 40.0 35.0 41.0 33.0 41.0 38-39 37.20240571445965 40.0 35.0 41.0 33.0 41.0 40-41 36.98482603975364 39.5 35.0 41.0 33.0 41.0 42-43 36.638265166350436 39.0 35.0 41.0 32.5 41.0 44-45 36.21054313747375 39.0 35.0 41.0 31.0 41.0 46-47 35.60406160901813 39.0 35.0 41.0 28.5 41.0 48-49 35.04927605345131 39.0 35.0 41.0 24.5 41.0 50-51 34.70468865732521 39.0 35.0 40.0 23.0 41.0 52-53 34.48493765029374 39.0 35.0 40.0 22.0 41.0 54-55 34.21721440384752 38.5 35.0 40.0 19.5 41.0 56-57 33.95873961260996 38.0 34.5 40.0 16.0 41.0 58-59 33.640974258550884 38.0 34.0 40.0 10.0 41.0 60-61 33.32058098359325 38.0 34.0 40.0 6.0 41.0 62-63 32.95529490548616 37.5 33.0 40.0 2.0 41.0 64-65 32.53032762766723 37.0 33.0 39.5 2.0 41.0 66-67 32.12780421481985 36.0 32.5 39.0 2.0 40.0 68-69 31.81587304808385 36.0 32.0 39.0 2.0 40.0 70-71 31.351055734245158 35.5 31.5 38.5 2.0 40.0 72-73 30.788492953316357 35.0 31.0 38.0 2.0 40.0 74-75 30.236421563156345 35.0 30.5 37.0 2.0 39.0 76-77 28.77798126972209 33.5 28.5 35.5 2.0 38.0 78-79 29.379557007619955 34.0 29.5 36.0 2.0 38.5 80-81 29.041930050630597 34.0 29.0 36.0 2.0 37.5 82-83 28.673123167304198 34.0 29.0 35.0 2.0 37.0 84-85 28.27026492283653 34.0 29.0 35.0 2.0 37.0 86-87 27.918722160780057 34.0 29.0 35.0 2.0 36.0 88-89 27.43658999360776 34.0 27.0 35.0 2.0 36.0 90-91 26.937340828150205 34.0 26.0 35.0 2.0 35.5 92-93 26.469342613918847 33.0 25.0 35.0 2.0 35.0 94-95 25.921431252980508 33.0 24.0 35.0 2.0 35.0 96-97 25.30146006879268 33.0 19.5 34.5 2.0 35.0 98-99 24.61451748734235 32.0 6.0 34.0 2.0 35.0 100-101 21.454655681483814 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.1650406698789908 1101 2 -0.06568321931936794 1101 3 -0.4883452696301731 1101 4 0.018330712193751708 1101 5 -0.05631107945117719 1101 6 -0.28797697818739465 1101 7 -0.29731421969990635 1101 8 -0.2817114450015836 1101 9 -0.14173265004475866 1101 10-11 -0.2136790322183657 1101 12-13 -0.15521072532077795 1101 14-15 -0.20037573491014626 1101 16-17 -0.15186530707267565 1101 18-19 -0.1694164385814929 1101 20-21 -0.22667796120369132 1101 22-23 -0.05143626075933838 1101 24-25 -0.2934613581865477 1101 26-27 0.046594481614967265 1101 28-29 -0.4269400774251153 1101 30-31 0.1194168317901898 1101 32-33 -0.27884606344733953 1101 34-35 -0.13654598461722856 1101 36-37 -0.030160976398370565 1101 38-39 0.006991889217161429 1101 40-41 0.18109385291055702 1101 42-43 0.7854710999551813 1101 44-45 0.5015877428234319 1101 46-47 1.1950693958340324 1101 48-49 1.0930620038506262 1101 50-51 0.4210626255872043 1101 52-53 0.8980124996539374 1101 54-55 0.8962355529971973 1101 56-57 0.8971188738123175 1101 58-59 0.9666160578745959 1101 60-61 0.970459648956286 1101 62-63 1.028368498154471 1101 64-65 1.576360780775353 1101 66-67 1.0255025962537587 1101 68-69 0.6328374989653014 1101 70-71 0.7742654682474281 1101 72-73 1.3527827863720034 1101 74-75 1.5025376660712588 1101 76-77 1.0272884438291996 1101 78-79 1.2165582131961408 1101 80-81 2.0257121976202015 1101 82-83 1.765922743130485 1101 84-85 1.5642489358680187 1101 86-87 1.1235560324808134 1101 88-89 1.9100641342895521 1101 90-91 1.7804221860053815 1101 92-93 1.111746324505308 1101 94-95 0.6026382650187365 1101 96-97 0.6898924271871891 1101 98-99 0.41682208426468037 1101 100-101 0.5119062501899272 1102 1 0.26032164896159316 1102 2 0.3071428676371539 1102 3 0.26962574486258006 1102 4 -0.12188667911059525 1102 5 0.050935297360410914 1102 6 -0.09957118108594187 1102 7 0.25413505566240957 1102 8 0.3385784100708804 1102 9 0.3068180745929183 1102 10-11 0.35081372140483325 1102 12-13 0.22594869496908387 1102 14-15 0.21864600422028246 1102 16-17 0.10519991031863185 1102 18-19 0.36609080779531666 1102 20-21 0.26806841560790673 1102 22-23 -0.07661742017962325 1102 24-25 -0.11809903934597088 1102 26-27 0.21307998886134527 1102 28-29 0.19570485011111316 1102 30-31 0.06996031005105863 1102 32-33 -0.05475186054879089 1102 34-35 -0.4579227962114274 1102 36-37 -0.4285305416157641 1102 38-39 -1.039928400637919 1102 40-41 -0.9464423789735008 1102 42-43 -0.7804709290303222 1102 44-45 -1.096600662973664 1102 46-47 -0.8576479954703231 1102 48-49 -1.3696191555696586 1102 50-51 -1.2628141860069988 1102 52-53 -1.4487266307808397 1102 54-55 -1.067170244104247 1102 56-57 -1.0016130102456486 1102 58-59 -1.2730578551688794 1102 60-61 -1.1856997713335709 1102 62-63 -0.3763416467730636 1102 64-65 -1.0808855960362465 1102 66-67 -1.1118162443259507 1102 68-69 -0.6446987329187586 1102 70-71 -0.8003722129119915 1102 72-73 -0.3624346049323428 1102 74-75 -0.5969188556678731 1102 76-77 -0.8739796721128315 1102 78-79 -0.6604345404270475 1102 80-81 -0.6520051936841469 1102 82-83 -0.15617870614487828 1102 84-85 -0.3457148322479213 1102 86-87 0.06739661219095794 1102 88-89 0.10100616327505563 1102 90-91 0.31248740339668757 1102 92-93 0.18638400566472768 1102 94-95 -0.03866608280734951 1102 96-97 -0.049238007595420186 1102 98-99 0.3074017944096106 1102 100-101 1.3133555255522467 1103 1 0.19008753524921929 1103 2 0.2174364388002914 1103 3 0.09370601242110865 1103 4 0.15102301988606115 1103 5 -0.02896065210074994 1103 6 -0.007635097845508199 1103 7 -0.30030567269135844 1103 8 0.3217073584172212 1103 9 0.17578871747660685 1103 10-11 0.22008165154232273 1103 12-13 0.20974653963649104 1103 14-15 -0.2737731708075799 1103 16-17 0.013412470705098656 1103 18-19 -0.11257883174388894 1103 20-21 0.09842887640314757 1103 22-23 0.21063638881331315 1103 24-25 0.12363265890747499 1103 26-27 0.08580388332436684 1103 28-29 0.14795308496805148 1103 30-31 0.26289973777309683 1103 32-33 -0.19198708908836437 1103 34-35 -0.08505025812150535 1103 36-37 0.004133895396499554 1103 38-39 0.6297482994735617 1103 40-41 0.8195553913720914 1103 42-43 0.9294881939722757 1103 44-45 1.2676133838490742 1103 46-47 1.4579967462613794 1103 48-49 1.2372927730813998 1103 50-51 1.1339899760145542 1103 52-53 0.7285680552095002 1103 54-55 0.5955945273561696 1103 56-57 -0.04572300652956329 1103 58-59 0.0603126390711779 1103 60-61 0.498878452375088 1103 62-63 0.8136249084108798 1103 64-65 0.3297795841941493 1103 66-67 0.9684513142024826 1103 68-69 0.7409571570849565 1103 70-71 0.5020432460252096 1103 72-73 1.1549195385087572 1103 74-75 1.2549949310285236 1103 76-77 1.3838055378462926 1103 78-79 1.5796992388371685 1103 80-81 1.6209045053125095 1103 82-83 1.1699825721903103 1103 84-85 1.1147831239022103 1103 86-87 1.1118038957286807 1103 88-89 0.8825000317254457 1103 90-91 1.4070247501079436 1103 92-93 1.6626010253600079 1103 94-95 1.5235784359589069 1103 96-97 1.9257898630846242 1103 98-99 1.79331781076041 1103 100-101 2.5281455664292416 1104 1 0.34189148351334353 1104 2 0.32922828510540825 1104 3 0.09779042358516676 1104 4 -0.03277548249651119 1104 5 0.05401340432462831 1104 6 -0.13281473629948692 1104 7 0.022892269975606894 1104 8 -0.11297988157975425 1104 9 -0.31857630786186775 1104 10-11 0.04369559905007492 1104 12-13 -0.2970986309254968 1104 14-15 0.21964638898365507 1104 16-17 0.3993515070866209 1104 18-19 0.051969992096971396 1104 20-21 0.10300492965176744 1104 22-23 -0.21474747019886564 1104 24-25 0.07851504299339496 1104 26-27 0.1014986114084806 1104 28-29 0.17877526180792103 1104 30-31 0.42771329196718 1104 32-33 0.37937665041696533 1104 34-35 0.1604304165627113 1104 36-37 0.49517088200870063 1104 38-39 0.3109373169457683 1104 40-41 0.8577900181022926 1104 42-43 1.20125281086964 1104 44-45 1.0661157664222571 1104 46-47 1.2712862099933133 1104 48-49 1.1258791129951717 1104 50-51 1.1626186727848449 1104 52-53 0.9473487828397893 1104 54-55 1.277577735888059 1104 56-57 1.8670672513934434 1104 58-59 1.2357532260161932 1104 60-61 1.2116838377468397 1104 62-63 1.5110381146736458 1104 64-65 1.3965672704508663 1104 66-67 1.1059598233924035 1104 68-69 1.0754628676968636 1104 70-71 1.3820825773919694 1104 72-73 1.6859686270799692 1104 74-75 2.0033120023544484 1104 76-77 1.7706144025312653 1104 78-79 1.6396039359100065 1104 80-81 2.158344940983035 1104 82-83 2.700804749030187 1104 84-85 2.4164244520922082 1104 86-87 2.636461637200579 1104 88-89 2.6060080870919116 1104 90-91 2.745577053262018 1104 92-93 2.8614513392545717 1104 94-95 2.4411338402399743 1104 96-97 2.21998092276241 1104 98-99 1.8175595473915216 1104 100-101 1.9936171646442027 1105 1 0.4163318791406212 1105 2 0.2848069767590644 1105 3 0.33126257350708244 1105 4 0.10803659454669656 1105 5 0.2495712734899982 1105 6 0.17084655122437198 1105 7 0.12327401559421247 1105 8 0.16534737852782655 1105 9 0.21022813426896647 1105 10-11 0.3206346932718347 1105 12-13 0.07714221585569447 1105 14-15 0.06898701018788955 1105 16-17 -0.024169228641298446 1105 18-19 0.21195611043810914 1105 20-21 0.26082203879631294 1105 22-23 0.2718480529661562 1105 24-25 0.1996758967154122 1105 26-27 0.2821336973012407 1105 28-29 0.5345795304180214 1105 30-31 0.3373089886529357 1105 32-33 -0.12222841638851634 1105 34-35 -0.02233029834271605 1105 36-37 -0.20099430973170485 1105 38-39 0.13125659509951504 1105 40-41 -0.27282771571690034 1105 42-43 -0.5909994882801115 1105 44-45 -1.1773338258040198 1105 46-47 -1.1326266825973406 1105 48-49 -1.2084085843846637 1105 50-51 -2.5840844332363204 1105 52-53 -2.6554188728950763 1105 54-55 -2.221901701904759 1105 56-57 -2.243812498736702 1105 58-59 -2.2708839421254012 1105 60-61 -1.9908148608476353 1105 62-63 -2.331925619492594 1105 64-65 -2.4197176505971996 1105 66-67 -2.205379756687414 1105 68-69 -1.8779468147601932 1105 70-71 -1.805146296458453 1105 72-73 -1.9550603508828992 1105 74-75 -0.7599623339287405 1105 76-77 -0.7641331247982492 1105 78-79 -1.3577064926862086 1105 80-81 -1.039728978850384 1105 82-83 -1.2179007862812803 1105 84-85 -1.7154079268770772 1105 86-87 -1.648502791048596 1105 88-89 -1.2502299833575101 1105 90-91 -1.5492346767397152 1105 92-93 -1.3274693617692037 1105 94-95 -0.9855970290989085 1105 96-97 -0.8973624747736011 1105 98-99 -0.7272955627941435 1105 100-101 -0.8273094360845796 1106 1 -0.3796240032123208 1106 2 0.028066780680632064 1106 3 0.08248806370315975 1106 4 0.009997378860418848 1106 5 -0.07089441278451147 1106 6 0.12243968847927533 1106 7 0.1526857803000965 1106 8 0.028705221665084935 1106 9 0.14160068328857278 1106 10-11 -0.0938873655516943 1106 12-13 0.08541427467923057 1106 14-15 -0.2024590682434848 1106 16-17 -0.014365307072672806 1106 18-19 -0.17254143858149718 1106 20-21 -0.08292796120369417 1106 22-23 0.22773040590733018 1106 24-25 0.049246975146786554 1106 26-27 0.1580528149482987 1106 28-29 0.391809922574879 1106 30-31 0.13816683179018696 1106 32-33 -0.08301273011400667 1106 34-35 -0.14487931795056141 1106 36-37 -0.029119309731704845 1106 38-39 -0.0982164441161757 1106 40-41 -0.010572813756112964 1106 42-43 -0.8572372333781502 1106 44-45 -0.7692455905099038 1106 46-47 -0.8090972708326447 1106 48-49 -1.026729662816031 1106 50-51 -1.2633123744127985 1106 52-53 -0.8374041670127212 1106 54-55 -0.5193894470027942 1106 56-57 -0.6080894595210111 1106 58-59 -0.47921727545873694 1106 60-61 -1.2472486843770483 1106 62-63 -0.674756501845529 1106 64-65 -1.5048892192246548 1106 66-67 -0.5463724037462399 1106 68-69 -0.24528750103470287 1106 70-71 0.08884880158076314 1106 72-73 -0.25867554696133155 1106 74-75 -0.2547540005954083 1106 76-77 -0.33000322283746897 1106 78-79 0.09051654652947505 1106 80-81 0.21946219762020291 1106 82-83 -0.2215772568695158 1106 84-85 -0.4742927307986484 1106 86-87 -1.001443967519183 1106 88-89 -0.5253525323771164 1106 90-91 0.1866721860053815 1106 92-93 0.09716299117197735 1106 94-95 0.6859715983520722 1106 96-97 1.0003090938538506 1106 98-99 0.5876554175980111 1106 100-101 0.9046145835232622 1107 1 0.15667650183818438 1107 2 0.1855794069432548 1107 3 0.4379173566324539 1107 4 0.1463610152240591 1107 5 0.19015356701346775 1107 6 0.2837401935297805 1107 7 0.1425847701990861 1107 8 0.33647037318023365 1107 9 0.32341886510675266 1107 10-11 0.47571490717557197 1107 12-13 0.44983977972973577 1107 14-15 0.8338414368070275 1107 16-17 0.9023013595939986 1107 18-19 0.35129063212557554 1107 20-21 0.3012260792003474 1107 22-23 0.25298293115985615 1107 24-25 0.3944174296922469 1107 26-27 -0.11388531636482924 1107 28-29 -0.3275713905564288 1107 30-31 -0.668335693462339 1107 32-33 -0.3229117200129963 1107 34-35 -0.7408389139101601 1107 36-37 -0.9333175420549438 1107 38-39 -0.8865687168434491 1107 40-41 -0.7391081672914623 1107 42-43 -1.0938218293377489 1107 44-45 -0.555230438994748 1107 46-47 0.07726636553099553 1107 48-49 -0.14304910726048092 1107 50-51 -0.6810838390592622 1107 52-53 -0.1906238639824167 1107 54-55 0.010124441886091518 1107 56-57 -0.15022961103615984 1107 58-59 -0.3946213158627714 1107 60-61 -0.25403530053866774 1107 62-63 -0.7127931180071485 1107 64-65 -0.6403058858913226 1107 66-67 -0.12323477748361 1107 68-69 -0.19999078386298308 1107 70-71 -0.05856281458085277 1107 72-73 0.0037928873821044817 1107 74-75 -0.9948108187772249 1107 76-77 -0.892029737988981 1107 78-79 -0.7944266352887084 1107 80-81 -0.5605251761171708 1107 82-83 -0.46210755989982033 1107 84-85 -0.6338571247380393 1107 86-87 -0.48881770489292364 1107 88-89 -1.0452388960134833 1107 90-91 -1.0611182180350198 1107 92-93 -1.1594657967068116 1107 94-95 -0.8645334521529797 1107 96-97 -1.570713633418876 1107 98-99 -1.9180264005838055 1107 100-101 -2.081023042739364 1108 1 0.33247706061746385 1108 2 0.43165720621254877 1108 3 0.3400235246960719 1108 4 -0.177945883550926 1108 5 0.10042650920129859 1108 6 0.14039181613885177 1108 7 0.21296946824335805 1108 8 0.4367282713104714 1108 9 0.06713259818219086 1108 10-11 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-1.4753720085623208 2316 100-101 -1.096110627447203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 7.0 6 9.0 7 26.0 8 44.0 9 87.0 10 132.0 11 213.0 12 380.0 13 517.0 14 627.0 15 970.0 16 1352.0 17 2078.0 18 1745.0 19 899.0 20 694.0 21 673.0 22 718.0 23 764.0 24 851.0 25 981.0 26 1074.0 27 1238.0 28 1482.0 29 1739.0 30 2151.0 31 2547.0 32 3089.0 33 4303.0 34 5924.0 35 8586.0 36 12525.0 37 21787.0 38 17213.0 39 1132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.134891556971915 25.01446245344105 18.305913874821123 17.544732114765914 2 14.382255053169901 25.640271125903073 41.335538598912244 18.641935222014773 3 16.537638118043365 32.086000994348446 34.160942398814896 17.21541848879329 4 10.672105199074638 22.828645643086166 42.05832217216608 24.44092698567312 5 9.505159450876143 40.737847134145724 37.80756313605325 11.949430278924886 6 23.22108018709985 43.28561137209939 19.55315198311637 13.940156457684386 7 21.21716367178384 38.78872124760291 25.70898058991244 14.285134490700813 8 22.225717097720104 35.74885599196404 23.359071400306423 18.666355510009435 9 24.60099231916556 19.58460586259728 25.386324664914717 30.42807715332244 10-11 20.334657554402146 30.530652419874478 30.264793477323348 18.869896548400025 12-13 23.644692918818553 29.809145976440032 30.210943920776813 16.33521718396461 14-15 21.646864251144006 30.32712034660146 30.549834106151767 17.476181296102762 16-17 16.19773329139483 33.648548555658145 32.28233408078574 17.871384072161288 18-19 15.48756297149351 31.446429205582582 37.02799916798993 16.03800865493397 20-21 16.839835624778043 30.435797270559583 36.99000558064025 15.73436152402212 22-23 17.09334063904701 29.66271955191623 36.60466956865849 16.639270240378274 24-25 17.158571421323146 30.509764525016365 37.08794414762724 15.243719906033254 26-27 17.17713908830319 30.101215088404658 37.51959615433398 15.20204966895817 28-29 16.039956573353493 29.26428361252879 38.94497600373388 15.75078381038384 30-31 16.68856634079275 30.442745458142873 37.43322290486071 15.435465296203663 32-33 16.40665033003049 31.280599483518767 36.902533193305025 15.410216993145715 34-35 16.89276256379557 29.655935144129792 37.444828880749206 16.006473411325427 36-37 17.527143581938105 29.73566717402334 36.74429223744292 15.992897006595635 38-39 16.884935635601607 30.335750275267532 37.21160335090648 15.567710738224386 40-41 16.15156711344704 30.360603508629524 38.02976957496677 15.458059802956663 42-43 16.287021723469667 30.847631319946835 36.466207372383494 16.399139584200007 44-45 16.55945290542529 30.691883884452654 35.83510050021815 16.91356270990391 46-47 17.3534977398751 31.073039322622048 34.39093311958115 17.182529817921697 48-49 17.71193749682918 31.28202526508041 34.29252701537212 16.713510222718277 50-51 17.692690153421545 31.556944557188082 33.821738777498176 16.9286265118922 52-53 17.291009263674827 31.81204785048246 34.28675791673854 16.61018496910417 54-55 17.412016579490743 31.490378517688004 33.91540806956486 17.182196833256388 56-57 17.713549355940113 32.255756966957364 32.71032778817812 17.320365888924403 58-59 17.3833416195704 31.093174508152643 33.845896283369015 17.677587588907944 60-61 17.353585778643613 32.36028247899671 32.91478956126466 17.371342181095013 62-63 18.192334035158925 31.543593516146217 33.57753481646838 16.68653763222648 64-65 18.04336576803271 31.168257962397394 33.698773298700246 17.08960297086965 66-67 17.374650574047863 31.650508094506087 33.09336458507729 17.88147674636876 68-69 17.006909707073063 31.60252442748866 33.82966202299177 17.560903842446503 70-71 17.404825682860505 31.905718576240922 33.296298551077555 17.393157189821014 72-73 17.63801657923841 30.47373601063344 33.40351268808913 18.484734722039022 74-75 17.529449962965593 31.66188094199296 32.88756760047485 17.921101494566596 76-77 17.976399443976582 31.027730146006878 33.378653976886476 17.617216433130068 78-79 17.41911917650461 30.485361040226067 33.28327693533085 18.81224284793847 80-81 17.733990147783253 32.480353908671674 31.754881717593463 18.030774225951614 82-83 17.52376990126739 31.824777521841924 31.758313123420834 18.89313945346985 84-85 16.939280785778067 30.60922152772141 32.006960792272096 20.444536894228428 86-87 17.348445260695968 31.467172652417215 32.06228089311897 19.12210119376785 88-89 18.18186430187607 29.80559473198251 32.62325354870785 19.389287417433565 90-91 17.368629321103523 29.34393295250464 33.29291678926916 19.99452093712268 92-93 17.75774424952058 30.01410351370273 32.50809176415678 19.720060472619906 94-95 17.26817983501933 29.869009811581115 32.51417961179825 20.348630741601305 96-97 17.788871301595034 29.84293193717277 31.32990381103129 21.0382929502009 98-99 17.543325621981413 30.00273955923536 31.484638175250616 20.969296643532612 100-101 17.242306482543096 30.72384508457035 31.401118134683482 20.632730298203068 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1704.0 1 1104.5 2 453.0 3 370.0 4 303.5 5 271.5 6 316.0 7 494.0 8 686.0 9 750.0 10 644.0 11 476.5 12 424.0 13 408.0 14 399.5 15 394.5 16 361.5 17 353.5 18 358.0 19 374.0 20 414.5 21 440.5 22 501.0 23 551.0 24 580.5 25 715.5 26 932.5 27 1125.0 28 1189.5 29 1334.0 30 1748.5 31 2113.0 32 2617.5 33 3424.5 34 4199.0 35 4760.0 36 5614.5 37 6273.0 38 5987.5 39 5516.5 40 4971.5 41 4192.0 42 3451.5 43 3306.5 44 3284.0 45 2862.5 46 2425.5 47 2328.5 48 3819.0 49 3545.0 50 1526.0 51 821.5 52 380.5 53 217.5 54 170.5 55 168.5 56 152.0 57 135.0 58 130.5 59 155.5 60 163.0 61 123.5 62 101.5 63 85.0 64 52.5 65 38.0 66 33.0 67 23.5 68 16.5 69 12.0 70 7.5 71 6.0 72 5.0 73 4.5 74 3.0 75 1.5 76 1.5 77 2.5 78 1.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026380673113020888 2 0.005073206367888633 3 0.0 4 0.0010146412735777266 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.008624450825410676 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.073206367888633E-4 20-21 0.002029282547155453 22-23 0.004058565094310906 24-25 0.011668374646143856 26-27 0.00456588573109977 28-29 0.0 30-31 0.00152196191036659 32-33 0.005580527004677496 34-35 0.0 36-37 0.007102488915044086 38-39 0.01674158101403249 40-41 0.0 42-43 0.0 44-45 0.0 46-47 5.073206367888633E-4 48-49 0.002029282547155453 50-51 0.005073206367888633 52-53 0.0 54-55 0.00152196191036659 56-57 0.0025366031839443165 58-59 0.0 60-61 0.0010146412735777266 62-63 0.00456588573109977 64-65 0.0 66-67 0.0025366031839443165 68-69 0.0 70-71 0.0010146412735777266 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.00152196191036659 80-81 5.073206367888633E-4 82-83 0.008117130188621813 84-85 0.005073206367888633 86-87 0.003551244457522043 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0010146412735777266 98-99 0.0010146412735777266 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 98557.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.01874042432298 #Duplication Level Percentage of deduplicated Percentage of total 1 67.83216783216784 19.684040707407895 2 11.0 6.384122893351056 3 5.416083916083917 4.715037998315696 4 2.8986013986013988 3.3645504631837415 5 2.1643356643356646 3.140314741723064 6 1.486013986013986 2.5873352476232028 7 1.2062937062937062 2.4503586756902096 8 1.0209790209790208 2.3702020150775693 9 0.6783216783216783 1.7715636636667107 >10 5.758741258741259 31.485333360390435 >50 0.4055944055944056 7.934494759377822 >100 0.1083916083916084 5.182787625435028 >500 0.01048951048951049 2.021165416966831 >1k 0.013986013986013986 6.90869243179074 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2165 2.196698357295778 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1941 1.9694187120143676 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1419 1.439775967206794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1284 1.302799395273801 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 829 0.8411376157959354 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 606 0.6148726117881023 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 557 0.5651551893827937 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 339 0.3439633917428493 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 291 0.29526061061111847 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 280 0.28409955660176345 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 246 0.24960175330012074 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 205 0.20800146108343395 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 203 0.20597217853627847 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 201 0.20394289598912305 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 185 0.18770863561187942 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 171 0.17350365778179125 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 167 0.16944509268748034 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 167 0.16944509268748034 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 161 0.16335724504601398 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 160 0.16234260377243626 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 155 0.15726939740454762 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 153 0.15524011485739217 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.15422547358381444 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 150 0.152196191036659 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 150 0.152196191036659 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 146 0.14813762594234808 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 137 0.13900585448014854 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.13799121320657082 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 134 0.13596193065941536 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 129 0.13088872429152673 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 127 0.12885944174437128 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 120 0.12175695282932719 No Hit GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT 116 0.1176983877350163 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 109 0.11059589881997219 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 107 0.10856661627281675 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 107 0.10856661627281675 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 103 0.10450805117850584 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 101 0.1024787686313504 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 99 0.10044948608419492 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.073206367888633E-4 0.0 18-19 0.0 0.0 0.0 0.0010146412735777266 0.0 20-21 0.0 0.0 0.0 0.002029282547155453 0.0 22-23 0.0 0.0 0.0 0.0076098095518329495 0.0 24-25 0.0 0.0 0.0 0.011161054009354993 0.0 26-27 0.0 0.0 0.0 0.017248901650821352 0.0 28-29 0.0 0.0 0.0 0.02587335247623203 0.0 30-31 0.0 0.0 0.0 0.043629574763842244 0.0 32-33 0.0 0.0 0.0 0.07203953042401859 0.0 34-35 0.0 0.0 0.0 0.1050153718152947 0.0 36-37 0.0 0.0 0.0 0.1395131751169374 0.0 38-39 0.0 0.0 0.0 0.20242093407875644 0.0 40-41 0.0 0.0 0.0 0.25670424221516486 0.0 42-43 0.0 0.0 0.0 0.31453879480909525 0.0 44-45 0.0 0.0 0.0 0.3607049727568818 0.0 46-47 0.0 0.0 0.0 0.4225980904451231 0.0 48-49 0.0 0.0 0.0 0.4682569477561208 0.0 50-51 0.0 0.0 0.0 0.49666690341629716 0.0 52-53 0.0 0.0 0.0 0.5600819830149051 0.0 54-55 0.0 0.0 0.0 0.6630680722830443 0.0 56-57 0.0 0.0 0.0 0.8193228284140142 0.0 58-59 0.0 0.0 0.0 1.012611991030571 0.0 60-61 0.0 0.0 0.0 1.2485160871373926 0.0 62-63 0.0 0.0 0.0 1.3920878273486408 0.0 64-65 0.0 0.0 0.0 1.532615643739156 0.0 66-67 0.0 0.0 0.0 1.8060614669683535 0.0 68-69 0.0 0.0 0.0 2.2093813732155 0.0 70-71 0.0 0.0 0.0 2.596974339722191 0.0 72-73 0.0 0.0 0.0 3.0966851669592215 0.0 74-75 0.0 0.0 0.0 3.5618981908946092 0.0 76-77 0.0 0.0 0.0 3.913471392189291 0.0 78-79 0.0 0.0 0.0 4.203151475795732 0.0 80-81 0.0 0.0 0.0 4.4202847083413666 0.0 82-83 0.0 0.0 0.0 4.710472112584595 0.0 84-85 0.0 0.0 0.0 5.431374737461571 0.0 86-87 0.0 0.0 0.0 6.446523331676086 0.0 88-89 0.0 0.0 0.0 7.513925951479854 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATAG 15 6.5907155E-4 95.033 1 CATTCCG 20 1.7372759E-5 94.98478 9 TAGGCAT 25 5.2556017E-5 75.98783 5 GCATTCC 30 1.2963827E-4 63.32319 8 GTAGATA 25 0.0050108763 56.99087 6 CCTTTAG 35 2.7706605E-4 54.304565 1 GGCATTC 35 2.7776204E-4 54.277016 7 GATTGGC 75 2.3501343E-9 50.65855 9 CATATAG 40 5.3549005E-4 47.5165 1 GGATATG 80 4.143658E-9 47.5165 1 TCCGGTG 20 5.381505E-4 47.49239 12-13 ATATGAG 85 7.1177055E-9 44.69872 3 AGGCATT 45 9.58979E-4 42.215458 6 GATATGA 100 2.9736839E-8 38.0132 2 CTTTAGG 50 0.0016059096 38.0132 2 CATGGGT 50 0.0016099223 37.993916 4 TTAGGCA 50 0.0016099223 37.993916 4 AGCCTTT 50 0.0016099223 37.993916 6 GTTGTAG 55 0.0025638693 34.55745 1 TAGGTGG 55 0.0025702643 34.539917 5 >>END_MODULE