FastQCFastQC Report
Thu 26 May 2016
SRR936947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252339
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32811.3002350013275792No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26441.0477968130174091No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24550.972897570331974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.7034188135801441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.5980050646154578No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT13670.5417315595290463No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13340.5286539139807956No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC11610.4600953479248155No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11550.4577175941887699No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9580.37964801318860736No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG9350.37053329053376605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.3582482295641974No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT7910.3134672008686727No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG7890.3126746162899908No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG7700.30514506279251324No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA7370.29206741724426266No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.2841415714574442No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT5980.2369827890258739No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG5460.21637558998014575No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5140.2036942367212361No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA5100.2021090675638724No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT5030.19933502153848592No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC4470.17714265333539403No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA4460.17674636104605312No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3970.15732803886834773No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.14583556247746088No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT3650.14464668560943808No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2950.11690622535557327No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC2560.10145082607127713No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGC156.5912714E-495.074638
GGTACTC704.2736538E-640.746273
TACTCCC756.863709E-638.0298545
TCCCGCT756.863709E-638.0298548
CCCGCTG756.863709E-638.0298549
ACTCCCG801.0685078E-535.652996
TGGTACT801.0685078E-535.652992
CTCCCGC902.3926674E-531.6915457
CCTAGAC650.005813176329.2537353
CAGGTCC700.00834731827.164189
ACAGTAC700.00834731827.164188
GAGTACT12000.026.92712412-13
TACAGCG458.9409895E-426.38866878-79
GCGGGAG458.946169E-426.38605182-83
CCGTTCC851.1170778E-725.15433316-17
CGTTCCA851.118151E-725.15183818-19
CTATACA950.001218307125.019644
GTGGTAC1151.2710792E-424.8020781
AAAGGTT1751.465396E-724.4477636
ATGGGAT3000.023.768665
Produced by FastQC (version 0.11.5)