##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936947_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 252339 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.042220980506386 33.0 31.0 34.0 31.0 34.0 2 32.166101950154356 33.0 31.0 34.0 30.0 34.0 3 32.15178787266336 34.0 31.0 34.0 30.0 34.0 4 35.70978723066985 37.0 35.0 37.0 35.0 37.0 5 35.75439389075807 37.0 35.0 37.0 35.0 37.0 6 35.82159317426161 37.0 35.0 37.0 35.0 37.0 7 35.79344849587262 37.0 35.0 37.0 35.0 37.0 8 35.78959653482022 37.0 35.0 37.0 35.0 37.0 9 37.4332861745509 39.0 37.0 39.0 35.0 39.0 10-11 37.34238068629898 39.0 37.0 39.0 35.0 39.0 12-13 37.27940191567693 39.0 37.0 39.0 34.0 39.0 14-15 38.42014314077491 40.0 38.0 41.0 34.0 41.0 16-17 38.35522848231942 40.0 38.0 41.0 34.0 41.0 18-19 38.41447219811444 40.0 38.0 41.0 34.0 41.0 20-21 38.370176627473356 40.0 38.0 41.0 34.0 41.0 22-23 38.25214096909316 40.0 38.0 41.0 34.0 41.0 24-25 38.10915078525318 40.0 38.0 41.0 34.0 41.0 26-27 38.01487681254186 40.0 38.0 41.0 34.0 41.0 28-29 38.06600049932828 40.0 38.0 41.0 34.0 41.0 30-31 37.96866120575892 40.0 37.5 41.0 34.0 41.0 32-33 37.781878742485304 40.0 37.0 41.0 33.0 41.0 34-35 37.60179361890155 40.0 37.0 41.0 33.0 41.0 36-37 37.42499772131934 40.0 37.0 41.0 33.0 41.0 38-39 37.20756997531099 39.5 36.0 41.0 32.5 41.0 40-41 37.00328130015574 39.0 36.0 41.0 31.5 41.0 42-43 36.843072216343884 39.0 36.0 41.0 31.0 41.0 44-45 36.58450338631761 39.0 36.0 41.0 31.0 41.0 46-47 36.17271408700201 39.0 36.0 41.0 30.0 41.0 48-49 35.75481396058477 39.0 35.0 41.0 28.5 41.0 50-51 35.059352696174585 38.5 35.0 39.5 27.0 40.5 52-53 35.290038400722835 38.5 35.5 40.0 28.0 40.5 54-55 35.63280547200393 39.0 36.0 41.0 28.0 41.0 56-57 35.59925338532688 39.0 36.0 41.0 27.5 41.0 58-59 35.41204094491934 39.0 35.5 41.0 26.5 41.0 60-61 35.13533976119427 39.0 35.0 41.0 26.0 41.0 62-63 34.810106642255064 39.0 35.0 40.0 25.5 41.0 64-65 34.426915775999746 38.0 34.5 40.0 23.0 41.0 66-67 34.07204197527929 38.0 34.0 40.0 22.0 41.0 68-69 33.66285235338176 37.0 34.0 40.0 22.0 41.0 70-71 33.26305684020306 37.0 33.5 39.0 20.5 41.0 72-73 32.83093180205992 36.0 33.0 39.0 19.0 40.5 74-75 32.350492789461796 36.0 33.0 38.5 15.5 40.0 76-77 31.838502966247788 35.0 32.0 37.5 10.5 39.5 78-79 31.382342800756128 35.0 32.0 37.0 7.0 39.0 80-81 30.97464323786652 35.0 31.0 36.5 3.5 39.0 82-83 30.539706505930514 35.0 31.0 36.0 2.0 37.5 84-85 30.14724834448896 35.0 31.0 36.0 2.0 37.0 86-87 29.764707397588168 34.0 31.0 35.0 2.0 37.0 88-89 29.297042866936938 34.0 30.0 35.0 2.0 36.0 90-91 28.890666920293732 34.0 29.0 35.0 2.0 36.0 92-93 28.60938657916533 34.0 29.0 35.0 2.0 36.0 94-95 28.282907120976148 34.0 29.0 35.0 2.0 35.0 96-97 27.822054854778692 34.0 29.0 35.0 2.0 35.0 98-99 27.256694367497694 34.0 27.5 35.0 2.0 35.0 100-101 25.21545421040743 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4995159628874042 1101 2 0.43298374137810214 1101 3 0.5173126403910189 1101 4 0.3548441687057533 1101 5 0.30515408438413516 1101 6 0.30475051543675846 1101 7 0.3876887983097106 1101 8 0.2180688908430497 1101 9 0.5101227320396191 1101 10-11 0.46682855830065506 1101 12-13 0.5690668783762547 1101 14-15 0.7049163578836328 1101 16-17 0.707551046694249 1101 18-19 0.8387211141142998 1101 20-21 0.8048321913051169 1101 22-23 0.808278570246614 1101 24-25 0.8569724821547524 1101 26-27 0.8164647600355437 1101 28-29 0.9047894425444625 1101 30-31 0.8344299944619991 1101 32-33 0.834448989376753 1101 34-35 0.8117482618775469 1101 36-37 0.7394970916039156 1101 38-39 0.8971596828217088 1101 40-41 0.9790023181383063 1101 42-43 1.1938361392361543 1101 44-45 1.3050357654157025 1101 46-47 1.1204447024942112 1101 48-49 1.2140812605757887 1101 50-51 1.3402520144645464 1101 52-53 1.2054429479004014 1101 54-55 1.1567589182641385 1101 56-57 1.1563108917835123 1101 58-59 1.0529452592043427 1101 60-61 1.479106221082965 1101 62-63 1.5476699015164215 1101 64-65 1.3196786964343872 1101 66-67 1.1128650914816376 1101 68-69 1.0723028400987928 1101 70-71 1.2719463490480507 1101 72-73 1.2968177627160316 1101 74-75 1.4103053286170848 1101 76-77 1.5141034479410251 1101 78-79 1.4320889477434449 1101 80-81 1.5699773831157238 1101 82-83 1.1808545382647786 1101 84-85 1.1710618097179193 1101 86-87 1.1668747127302943 1101 88-89 1.1526525825287663 1101 90-91 1.2765242244750503 1101 92-93 0.9982293675748544 1101 94-95 0.8693672122809879 1101 96-97 0.7955904775039251 1101 98-99 1.1540060812211657 1101 100-101 1.0792486771995904 1102 1 0.14936955340564495 1102 2 0.10236789197071516 1102 3 0.25215809704916836 1102 4 0.13961758260303725 1102 5 0.08399675229024695 1102 6 0.1366910220600417 1102 7 0.046698791337831835 1102 8 0.12469217692252954 1102 9 0.3487655393833293 1102 10-11 0.1986063877219877 1102 12-13 0.2388275104933868 1102 14-15 0.4306658349926309 1102 16-17 0.28087198557410176 1102 18-19 0.372999524525639 1102 20-21 0.41751636513733104 1102 22-23 0.353428465668415 1102 24-25 0.4129427354663946 1102 26-27 0.39142176677502505 1102 28-29 0.37429443905852366 1102 30-31 0.4428985048036225 1102 32-33 0.6885693705061939 1102 34-35 0.4288944389633045 1102 36-37 0.5531154973673367 1102 38-39 0.40826821175501493 1102 40-41 0.07564651985803295 1102 42-43 0.3637176172747374 1102 44-45 -0.09824101822362508 1102 46-47 0.28569685215722984 1102 48-49 0.17191997774985168 1102 50-51 0.19304309045804047 1102 52-53 0.304550547249697 1102 54-55 0.48484629916118394 1102 56-57 0.5790020374958047 1102 58-59 0.4174072624578855 1102 60-61 0.34391217042063005 1102 62-63 0.5554923509702903 1102 64-65 0.6740593610869539 1102 66-67 0.6394186587602846 1102 68-69 0.630778322320495 1102 70-71 0.8869451870680365 1102 72-73 0.5342335191650136 1102 74-75 0.6395872249684658 1102 76-77 0.581535472110204 1102 78-79 0.7941386804880395 1102 80-81 0.8358384103060565 1102 82-83 0.6468782426570634 1102 84-85 0.7053448680493162 1102 86-87 0.7549574457072872 1102 88-89 0.7224689896865613 1102 90-91 0.8478047264504163 1102 92-93 0.9900908595571956 1102 94-95 1.113750200893584 1102 96-97 1.0909936845687653 1102 98-99 0.962683747965297 1102 100-101 0.9613774245851374 1103 1 0.2717581887466558 1103 2 0.4381836478546077 1103 3 0.3789820348263717 1103 4 0.4090690927179139 1103 5 0.32297966306077797 1103 6 0.18189804875450477 1103 7 0.14276946232654808 1103 8 0.20305836944955047 1103 9 0.1952104103187864 1103 10-11 0.1425498582772704 1103 12-13 0.1702639791478262 1103 14-15 0.3867260375656585 1103 16-17 0.11437826203209767 1103 18-19 0.40325232758168283 1103 20-21 0.1286385976647182 1103 22-23 0.17746725390338725 1103 24-25 0.27141251021180324 1103 26-27 0.3890296419621251 1103 28-29 0.5363115374708158 1103 30-31 0.5394007683689495 1103 32-33 0.8095530342680277 1103 34-35 0.5321644414426672 1103 36-37 0.6940212908089691 1103 38-39 0.6533439241123347 1103 40-41 0.3720909316524441 1103 42-43 0.4648204740502777 1103 44-45 0.8450872033394816 1103 46-47 0.8305732973036513 1103 48-49 0.8656407649012507 1103 50-51 0.42338037546524987 1103 52-53 0.44770154037176013 1103 54-55 0.5125784179134243 1103 56-57 0.5082070807009771 1103 58-59 0.402260199583111 1103 60-61 0.5150753583286942 1103 62-63 0.3507889101673456 1103 64-65 0.5816847754617385 1103 66-67 0.4928837961811965 1103 68-69 0.8161840652566141 1103 70-71 0.7036882241941811 1103 72-73 0.3430885132421011 1103 74-75 0.5721945032722147 1103 76-77 0.5882067914060656 1103 78-79 0.8192996094221883 1103 80-81 1.1262083861552377 1103 82-83 1.141855239691015 1103 84-85 0.7254644283758545 1103 86-87 0.9356799500461719 1103 88-89 0.8985679437632754 1103 90-91 0.8988015216459644 1103 92-93 1.006955109917623 1103 94-95 0.7875855896483053 1103 96-97 0.8483330540762886 1103 98-99 1.0150441920465099 1103 100-101 1.411537664807728 1104 1 0.3431057064771501 1104 2 0.3506760490704153 1104 3 0.33962033269871483 1104 4 0.2681775020390873 1104 5 0.23130793053798016 1104 6 0.3468017974880411 1104 7 0.21640674702766205 1104 8 0.3201201728943346 1104 9 0.3685842705011524 1104 10-11 0.29657214804424115 1104 12-13 0.45586175017113106 1104 14-15 0.2520958450631241 1104 16-17 0.3624228415660369 1104 18-19 0.5830800884732739 1104 20-21 0.31457578104870976 1104 22-23 0.44238113434917636 1104 24-25 0.39299812318039784 1104 26-27 0.2615929651637501 1104 28-29 0.4241484169034422 1104 30-31 0.2582761483081555 1104 32-33 0.19009001501778044 1104 34-35 0.43764569777498963 1104 36-37 0.3474458095526387 1104 38-39 0.5533135289755577 1104 40-41 0.7022074463434294 1104 42-43 0.7439643443643646 1104 44-45 0.8159332013131362 1104 46-47 1.053521625571129 1104 48-49 0.8715171580116845 1104 50-51 0.9992263734389013 1104 52-53 0.9262121786696369 1104 54-55 1.031246097751314 1104 56-57 1.038105763578379 1104 58-59 0.8807657720248514 1104 60-61 1.044234426211169 1104 62-63 0.9192083630548851 1104 64-65 0.9396786964343846 1104 66-67 0.8044035530201015 1104 68-69 1.041533609329555 1104 70-71 1.0043822464839423 1104 72-73 1.077586993485255 1104 74-75 1.1206899440016969 1104 76-77 1.1207701146076943 1104 78-79 0.9801658708203718 1104 80-81 0.6999773831157263 1104 82-83 0.8698288972391417 1104 84-85 0.6940105276666344 1104 86-87 0.8661054819610641 1104 88-89 1.0375243774005583 1104 90-91 0.9804985834494104 1104 92-93 1.0059216752671638 1104 94-95 1.071162084075862 1104 96-97 0.8953340672475143 1104 98-99 1.044390696605781 1104 100-101 1.6733512413021536 1105 1 0.32078686589743555 1105 2 0.08531373022983502 1105 3 0.139620332698712 1105 4 0.23875721218401225 1105 5 0.24594561169740103 1105 6 0.1131786090822402 1105 7 0.26843573253490405 1105 8 -0.1179957691346516 1105 9 0.03249731397941247 1105 10-11 0.13548519152249838 1105 12-13 -0.05493535127813942 1105 14-15 -0.20717951725571737 1105 16-17 0.19445182707328712 1105 18-19 0.17721052325588005 1105 20-21 0.34551781003421667 1105 22-23 0.18491736623323618 1105 24-25 0.2979256594122788 1105 26-27 0.11644803762752076 1105 28-29 -0.25215593092264754 1105 30-31 -0.5549122574889367 1105 32-33 -0.24404041976483626 1105 34-35 -0.028223867442399353 1105 36-37 -0.07335129189663547 1105 38-39 -0.1222661811693655 1105 40-41 -0.19670559713483016 1105 42-43 0.0475150690020385 1105 44-45 0.1626723317479204 1105 46-47 0.45243466904939567 1105 48-49 0.6907200565624123 1105 50-51 0.34784956184470417 1105 52-53 0.18193681635079173 1105 54-55 0.556970735432472 1105 56-57 0.4131782273464992 1105 58-59 0.7007657720248517 1105 60-61 0.9176402233126169 1105 62-63 0.7418895224751765 1105 64-65 0.8080844935358371 1105 66-67 0.8675919588172007 1105 68-69 1.024649551358543 1105 70-71 1.1305416667737944 1105 72-73 0.7710652543548235 1105 74-75 0.5119218280596698 1105 76-77 0.5819295348975473 1105 78-79 0.7928470302406652 1105 80-81 1.0500498468838408 1105 82-83 0.9170028102826215 1105 84-85 1.2018366146231578 1105 86-87 1.1112504094972984 1105 88-89 0.9752055368208516 1105 90-91 0.9316580037392654 1105 92-93 1.1488926897599185 1105 94-95 0.9852200550903554 1105 96-97 1.2858413136243243 1105 98-99 0.8095356241420149 1105 100-101 0.7161773282586772 1106 1 0.335883484254925 1106 2 0.39308345647781806 1106 3 0.5794351475135286 1106 4 0.4074367612983494 1106 5 0.25278941201946026 1106 6 0.30661661230285375 1106 7 0.15511045073136387 1106 8 0.3630831358572948 1106 9 0.2011768630937496 1106 10-11 0.3712943702664617 1106 12-13 0.5221580464674318 1106 14-15 0.5291328821001571 1106 16-17 0.8427006193438231 1106 18-19 0.718172681065866 1106 20-21 0.560594299567235 1106 22-23 0.602288541756586 1106 24-25 0.7988314565137244 1106 26-27 0.5372411133119002 1106 28-29 0.7757224909775147 1106 30-31 0.7025354075674173 1106 32-33 0.7728677927955587 1106 34-35 0.6446827348120294 1106 36-37 0.7259643280711501 1106 38-39 0.5423876030496331 1106 40-41 0.7827630018989851 1106 42-43 0.635075455475473 1106 44-45 0.5506554235353533 1106 46-47 0.5655586626081686 1106 48-49 0.8719801209746549 1106 50-51 0.9229300771426026 1106 52-53 0.7747306971881542 1106 54-55 0.7678201718253845 1106 56-57 0.9608835413561607 1106 58-59 0.8095620683211493 1106 60-61 0.967660352137095 1106 62-63 0.9606898445363683 1106 64-65 0.9460675853232701 1106 66-67 0.9712554048719539 1106 68-69 0.872274350070299 1106 70-71 1.0442896538913544 1106 72-73 1.2623092157074822 1106 74-75 1.3205973514091056 1106 76-77 1.0581775220151002 1106 78-79 1.6898880930425904 1106 80-81 1.7366440497823952 1106 82-83 1.482421489831733 1106 84-85 1.6595660832221881 1106 86-87 2.0052721486277285 1106 88-89 1.9046540070301887 1106 90-91 1.690313398264223 1106 92-93 1.6325883419338325 1106 94-95 2.1567176396314167 1106 96-97 1.5457044376178857 1106 98-99 1.4350388447539295 1106 100-101 1.4169623524132646 1107 1 -0.1095452121580216 1107 2 -0.0015286753390384433 1107 3 -0.24817494289183983 1107 4 -0.007990476963534832 1107 5 -0.2601881324541466 1107 6 0.01509576074263208 1107 7 -0.11125729496708914 1107 8 -0.12087720243374633 1107 9 -0.04495903658546041 1107 10-11 -0.22970081783502394 1107 12-13 -0.23444008709920894 1107 14-15 -0.488875283545795 1107 16-17 -0.34665852326335056 1107 18-19 -0.05437397976819369 1107 20-21 -0.11130348404315527 1107 22-23 0.06282732857489748 1107 24-25 -0.12816985582222884 1107 26-27 -0.35877448890448704 1107 28-29 -0.06491982456637402 1107 30-31 0.0348115813790244 1107 32-33 0.18833148483405182 1107 34-35 0.08664832244689791 1107 36-37 -0.05698988598542343 1107 38-39 0.04016392267634217 1107 40-41 0.4050289686531414 1107 42-43 0.23229767769769438 1107 44-45 0.2734791330716675 1107 46-47 0.3995846176971156 1107 48-49 0.3107560031560439 1107 50-51 0.3281502579533395 1107 52-53 0.3467633597719981 1107 54-55 0.4130702447329426 1107 56-57 3.629814261429942E-4 1107 58-59 0.20831170378338726 1107 60-61 0.022213428835833327 1107 62-63 0.18590127644071686 1107 64-65 0.3972377515524954 1107 66-67 0.22078150577600297 1107 68-69 0.22995880617995823 1107 70-71 0.10751872942357465 1107 72-73 -0.13461773092419094 1107 74-75 0.13120175502532305 1107 76-77 0.16914281801976472 1107 78-79 0.2673705952298171 1107 80-81 0.29044982406060527 1107 82-83 0.3373354589189326 1107 84-85 0.10827561953015419 1107 86-87 0.5635070567642124 1107 88-89 0.718757973201086 1107 90-91 0.7088319167827422 1107 92-93 0.9954492343222832 1107 94-95 1.1379074909000089 1107 96-97 0.9633918100296661 1107 98-99 0.9556899092042066 1107 100-101 1.358836805606618 1108 1 -0.5136814421172282 1108 2 -0.19956491478500737 1108 3 -0.21881340224104662 1108 4 -0.27551727707737683 1108 5 -0.3190535152451517 1108 6 -0.16934278082521104 1108 7 -0.18262939755065588 1108 8 -0.23240994758759115 1108 9 -0.5520582997799721 1108 10-11 -0.6172431129999367 1108 12-13 -0.6260458803509508 1108 14-15 -0.1564985324469177 1108 16-17 -0.08994663634560851 1108 18-19 -0.06442995168737298 1108 20-21 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-1.85137120945776 2316 98-99 -1.9575591103440253 2316 100-101 -1.7328392348883206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 651.0 3 123.0 4 142.0 5 105.0 6 157.0 7 171.0 8 188.0 9 234.0 10 326.0 11 440.0 12 656.0 13 940.0 14 1113.0 15 1608.0 16 2220.0 17 3414.0 18 2818.0 19 1525.0 20 1321.0 21 1368.0 22 1359.0 23 1522.0 24 1641.0 25 1955.0 26 2171.0 27 2581.0 28 3044.0 29 3760.0 30 4667.0 31 5945.0 32 7418.0 33 10147.0 34 14092.0 35 20998.0 36 31692.0 37 50705.0 38 57062.0 39 12055.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.970611212232974 22.109203675283833 15.97011551685166 20.950069595631536 2 13.516922930451756 23.143079291029974 41.12597388172197 22.214023896796306 3 17.728029822335024 28.797588832487307 33.14522525380711 20.32915609137056 4 10.662555457669628 19.2365691246617 42.070446121733056 28.030429295935615 5 10.902598690735966 39.94604245189446 36.80460226145606 12.34675659591351 6 24.434482485024 41.05169199032015 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17.879188668806897 28.74657698584394 34.44786608157332 18.926368263775835 30-31 18.16516489725518 30.501283690685067 33.01711918002399 18.316432232035766 32-33 17.52101081915925 30.513915020926557 32.5548244092287 19.41024975068549 34-35 18.33486312191853 29.072591276834903 32.33887163058282 20.253673970663744 36-37 19.38022148824558 28.960995705840137 32.36579263529776 19.292990170616527 38-39 19.602295819744448 28.6848600798977 33.10441221661595 18.608431883741908 40-41 17.828327592941797 29.347379942536598 34.302953872632706 18.5213385918889 42-43 17.56936554297759 30.35764919565118 32.49735783360202 19.57562742776922 44-45 18.213232830488682 30.856844905897535 31.178813513958254 19.75110874965553 46-47 18.362132363872792 29.987927996146475 30.12133454119447 21.528605098786258 48-49 18.846656318332215 29.864460607934912 30.989891540732195 20.29899153300068 50-51 20.105168852894423 30.130697967581195 30.767934797689694 18.996198381834688 52-53 18.443980564625 30.620825825022152 31.76407151678789 19.171122093564957 54-55 18.105887134346407 29.823455358293433 31.951903728154956 20.118753779205203 56-57 19.20383668322779 31.65719655666007 29.30447229745001 19.834494462662125 58-59 18.373509503094997 29.78733528695659 30.787608899956776 21.051546309991632 60-61 18.83676390700773 29.351330132081383 31.054192622317032 20.757713338593856 62-63 20.639465142884333 29.52777684149584 30.427532769317096 19.40522524630273 64-65 20.078696453703888 29.41106507820088 31.43516958391976 19.075068884175472 66-67 18.417259054360866 30.01655711212238 31.09009250171024 20.476091331806508 68-69 18.177672834402923 30.834121399923475 31.379126445532208 19.609079320141394 70-71 18.619954254817316 30.52460290089469 30.244344286014417 20.611098558273575 72-73 18.42660197715255 29.591885271244085 30.126009782703484 21.85550296889988 74-75 18.779595841468367 30.334882706811626 30.37531838138373 20.51020307033627 76-77 19.657074207412812 29.040648343457093 30.997732066674068 20.30454538245603 78-79 18.313584594397387 29.163890739506996 31.702476127026426 20.82004853906919 80-81 18.439775360638155 30.965421952094648 30.42681875219297 20.16798393507423 82-83 18.19937425400212 30.786465169581923 30.009636012514918 21.00452456390104 84-85 18.398443928485744 29.087116608208234 30.238902977513497 22.275536485792518 86-87 19.05494605725208 29.682688551764613 30.29736423103081 20.965001159952493 88-89 20.249893398649384 27.909522723441388 30.937992721358942 20.902591156550283 90-91 18.245176436064853 27.94940784816803 32.60671720065748 21.198698515109637 92-93 18.33211704887084 29.226125790254386 31.57210054970769 20.869656611167077 94-95 17.88087744453501 29.195794834571835 31.181765717311507 21.74156200358165 96-97 17.848819566066815 28.348661883973747 30.123626727808972 23.678891822150465 98-99 18.039730737258527 28.45570715196918 31.001323299797832 22.50323881097446 100-101 18.587543496978522 28.546516472310724 31.204345531665005 21.661594499045744 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 638.0 1 418.0 2 191.0 3 175.5 4 148.5 5 136.0 6 214.5 7 374.5 8 505.0 9 535.0 10 464.0 11 373.5 12 357.5 13 393.0 14 421.0 15 425.5 16 437.5 17 480.5 18 507.0 19 567.5 20 692.0 21 795.5 22 897.0 23 1081.5 24 1307.0 25 1653.0 26 2294.0 27 3005.0 28 3440.0 29 3879.5 30 4600.5 31 5427.5 32 6676.0 33 8136.0 34 9641.5 35 10823.5 36 12209.5 37 13824.0 38 13593.0 39 12062.5 40 10777.0 41 9572.5 42 8217.5 43 7920.5 44 8180.0 45 7780.0 46 7339.0 47 8556.5 48 18027.0 49 17356.0 50 6817.5 51 4265.5 52 3077.0 53 2411.5 54 1950.0 55 1564.5 56 1248.0 57 983.5 58 744.5 59 577.5 60 446.5 61 316.0 62 221.5 63 153.5 64 98.5 65 66.0 66 57.5 67 41.0 68 24.5 69 14.5 70 9.5 71 8.5 72 8.0 73 5.5 74 4.0 75 5.0 76 3.5 77 2.0 78 1.0 79 0.5 80 1.0 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06657710460927561 2 0.1010545337819362 3 0.0709363197920258 4 0.13672083982261957 5 0.1145284716195277 6 0.10660262583270919 7 0.11373588704084583 8 0.10145082607127713 9 0.11888768680227788 10-11 0.10997111029210704 12-13 0.09728975703319741 14-15 0.10164897221594758 16-17 0.11571734848755048 18-19 0.09768604932253833 20-21 0.08123991931488989 22-23 0.07668255798746924 24-25 0.07271963509405997 26-27 0.06063272026916172 28-29 0.07351221967274182 30-31 0.05409389749503644 32-33 0.058453112677786634 34-35 0.04973468231228625 36-37 0.05389575135036598 38-39 0.05607535894174107 40-41 0.07073817364735535 42-43 0.07073817364735535 44-45 0.05567906665240014 46-47 0.04061995965744494 48-49 0.04933839002294533 50-51 0.030910798568592253 52-53 0.04696063628689977 54-55 0.05409389749503644 56-57 0.05627350508641153 58-59 0.06261418171586636 60-61 0.05785867424377524 62-63 0.06320862014987774 64-65 0.041214398091456335 66-67 0.07192705051537812 68-69 0.05528277436305922 70-71 0.028533044832546695 72-73 0.0225886604924328 74-75 0.034279283027990125 76-77 0.05032912074629764 78-79 0.0677659814772984 80-81 0.04458288255085421 82-83 0.06340676629454821 84-85 0.06558637388592331 86-87 0.06875671220065072 88-89 0.09174166498242443 90-91 0.06558637388592331 92-93 0.08123991931488989 94-95 0.08738244979967426 96-97 0.09887492619056111 98-99 0.1258228018657441 100-101 0.12285060969568715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 252339.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.538326714939394 #Duplication Level Percentage of deduplicated Percentage of total 1 78.8013552939943 33.52077797075948 2 11.508316390903373 9.790890451502797 3 3.2466881907281966 4.143260489961946 4 1.519916540382067 2.58618825496851 5 0.815235401591859 1.7339374931249663 6 0.5650160419526189 1.4420902195057446 7 0.40158168363872315 1.1957829002951605 8 0.369693016593465 1.258089785926746 9 0.28100638476458245 1.0758187263689798 >10 2.1185088743689557 17.95489523450943 >50 0.25111205784125645 7.425606834746645 >100 0.09964764200049861 6.871589930247623 >500 0.01295419346006482 3.9302561121001762 >1k 0.008968287780044874 7.070815595981777 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3281 1.3002350013275792 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2644 1.0477968130174091 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2455 0.972897570331974 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1775 0.7034188135801441 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1509 0.5980050646154578 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1367 0.5417315595290463 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1334 0.5286539139807956 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1161 0.4600953479248155 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1155 0.4577175941887699 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 958 0.37964801318860736 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 935 0.37053329053376605 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 904 0.3582482295641974 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 791 0.3134672008686727 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 789 0.3126746162899908 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 770 0.30514506279251324 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 737 0.29206741724426266 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 717 0.2841415714574442 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 598 0.2369827890258739 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 546 0.21637558998014575 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 514 0.2036942367212361 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 510 0.2021090675638724 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 503 0.19933502153848592 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 447 0.17714265333539403 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 446 0.17674636104605312 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 397 0.15732803886834773 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 368 0.14583556247746088 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 365 0.14464668560943808 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 295 0.11690622535557327 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 256 0.10145082607127713 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9814614467046315E-4 0.0 22-23 0.0 0.0 0.0 0.0031703383147274103 0.0 24-25 0.0 0.0 0.0 0.004755507472091115 0.0 26-27 0.0 0.0 0.0 0.010898037956875474 0.0 28-29 0.0 0.0 0.0 0.023975683505126043 0.0 30-31 0.0 0.0 0.0 0.05032912074629764 0.0 32-33 0.0 0.0 0.0 0.08203250389357175 0.0 34-35 0.0 0.0 0.0 0.11036740258144798 0.0 36-37 0.0 0.0 0.0 0.1301820170484943 0.0 38-39 0.0 0.0 0.0 0.14345780874141534 0.0 40-41 0.0 0.0 0.0 0.16109281561708655 0.0 42-43 0.0 0.0 0.0 0.1791241147820987 0.0 44-45 0.0 0.0 0.0 0.21201637479739555 0.0 46-47 0.0 0.0 0.0 0.2457012193913743 0.0 48-49 0.0 0.0 0.0 0.2813675254320577 0.0 50-51 0.0 0.0 0.0 0.31287276243466133 0.0 52-53 0.0 0.0 0.0 0.39966077380032416 0.0 54-55 0.0 0.0 0.0 0.497148676978192 0.0 56-57 0.0 0.0 0.0 0.6217826019759134 0.0 58-59 0.0 0.0 0.0 0.7947641862732278 0.0 60-61 0.0 0.0 0.0 1.0470042284387273 0.0 62-63 0.0 0.0 0.0 1.2078988979111434 0.0 64-65 0.0 0.0 0.0 1.3541307526779451 0.0 66-67 0.0 0.0 0.0 1.6640313229425496 0.0 68-69 0.0 0.0 0.0 2.0343664673316453 0.0 70-71 0.0 0.0 0.0 2.311771069870294 0.0 72-73 0.0 0.0 0.0 2.552914927934247 0.0 74-75 0.0 0.0 0.0 3.100392725658737 0.0 76-77 0.0 0.0 0.0 3.4235690876162623 0.0 78-79 0.0 0.0 0.0 3.6221115245760664 0.0 80-81 0.0 0.0 0.0 3.845422229619678 0.0 82-83 0.0 0.0 0.0 4.244488564985991 0.0 84-85 0.0 0.0 0.0 4.793947824157185 0.0 86-87 0.0 0.0 0.0 5.571275149699412 0.0 88-89 0.0 0.0 0.0 6.6083720709046165 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTCCGC 15 6.5912714E-4 95.07463 8 GGTACTC 70 4.2736538E-6 40.74627 3 TACTCCC 75 6.863709E-6 38.029854 5 TCCCGCT 75 6.863709E-6 38.029854 8 CCCGCTG 75 6.863709E-6 38.029854 9 ACTCCCG 80 1.0685078E-5 35.65299 6 TGGTACT 80 1.0685078E-5 35.65299 2 CTCCCGC 90 2.3926674E-5 31.691545 7 CCTAGAC 65 0.0058131763 29.253735 3 CAGGTCC 70 0.008347318 27.16418 9 ACAGTAC 70 0.008347318 27.16418 8 GAGTACT 1200 0.0 26.927124 12-13 TACAGCG 45 8.9409895E-4 26.388668 78-79 GCGGGAG 45 8.946169E-4 26.386051 82-83 CCGTTCC 85 1.1170778E-7 25.154333 16-17 CGTTCCA 85 1.118151E-7 25.151838 18-19 CTATACA 95 0.0012183071 25.01964 4 GTGGTAC 115 1.2710792E-4 24.802078 1 AAAGGTT 175 1.465396E-7 24.447763 6 ATGGGAT 300 0.0 23.76866 5 >>END_MODULE