##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936947_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 252339 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24257843615137 33.0 31.0 34.0 31.0 34.0 2 32.259872631658205 33.0 31.0 34.0 30.0 34.0 3 32.420129270544784 34.0 31.0 34.0 31.0 34.0 4 36.11024455197175 37.0 35.0 37.0 35.0 37.0 5 36.05484288992189 37.0 35.0 37.0 35.0 37.0 6 36.128981251411794 37.0 35.0 37.0 35.0 37.0 7 36.082500128795 37.0 35.0 37.0 35.0 37.0 8 36.08365333935698 37.0 35.0 37.0 35.0 37.0 9 37.76936581344937 39.0 37.0 39.0 35.0 39.0 10-11 37.718462068883525 39.0 37.0 39.0 35.0 39.0 12-13 37.64184688058525 39.0 37.0 39.0 35.0 39.0 14-15 38.78776170152057 40.0 38.0 41.0 35.0 41.0 16-17 38.71181624719128 40.0 38.0 41.0 34.5 41.0 18-19 38.859849250413134 40.0 38.0 41.0 35.0 41.0 20-21 38.97182361822786 40.0 38.0 41.0 35.0 41.0 22-23 38.920222399232784 40.0 38.0 41.0 35.0 41.0 24-25 38.83096152398163 40.0 38.0 41.0 35.0 41.0 26-27 38.60749032056083 40.0 38.0 41.0 34.5 41.0 28-29 38.41873234022486 40.0 38.0 41.0 34.0 41.0 30-31 38.27444429913727 40.0 38.0 41.0 34.0 41.0 32-33 38.20784737991353 40.0 37.0 41.0 34.0 41.0 34-35 38.1077776324706 40.0 37.0 41.0 34.0 41.0 36-37 37.9110086035056 40.0 37.0 41.0 33.5 41.0 38-39 37.69152608197702 40.0 37.0 41.0 33.0 41.0 40-41 37.48059158512953 40.0 37.0 41.0 33.0 41.0 42-43 37.25473866504979 40.0 37.0 41.0 33.0 41.0 44-45 36.83951351158561 40.0 36.5 41.0 31.5 41.0 46-47 36.41912268812986 40.0 36.0 41.0 30.5 41.0 48-49 35.96594264065404 40.0 36.0 41.0 29.5 41.0 50-51 35.68640598559874 39.5 36.0 41.0 28.0 41.0 52-53 35.40371880684317 39.0 35.0 41.0 27.0 41.0 54-55 35.19568715101511 39.0 35.0 40.0 26.0 41.0 56-57 35.00722044551179 39.0 35.0 40.0 25.5 41.0 58-59 34.759694300128004 39.0 35.0 40.0 24.0 41.0 60-61 34.48233130827974 38.5 34.5 40.0 23.5 41.0 62-63 34.08587852056162 38.0 34.0 40.0 22.0 41.0 64-65 33.610561585803225 37.5 34.0 40.0 20.0 41.0 66-67 33.23700260364034 37.0 33.0 39.5 19.0 41.0 68-69 32.84456029389037 36.5 33.0 39.0 17.5 40.0 70-71 32.353312409100454 36.0 32.0 39.0 13.0 40.0 72-73 31.994519277638414 35.5 32.0 38.0 9.5 40.0 74-75 31.55199949274587 35.0 31.5 37.5 7.5 39.5 76-77 30.067544057795267 34.0 29.5 36.0 4.0 38.5 78-79 30.690364945569254 34.5 31.0 36.0 2.0 39.0 80-81 30.535699990885277 35.0 31.0 36.0 2.0 38.5 82-83 30.207615548924267 34.0 31.0 36.0 2.0 37.0 84-85 29.84950404019989 34.0 31.0 35.0 2.0 37.0 86-87 29.493029218630493 34.0 30.0 35.0 2.0 36.5 88-89 29.04993679137985 34.0 29.5 35.0 2.0 36.0 90-91 28.608821862653016 34.0 29.0 35.0 2.0 36.0 92-93 28.27089748314767 34.0 29.0 35.0 2.0 35.0 94-95 27.895775127903335 34.0 29.0 35.0 2.0 35.0 96-97 27.317432105223528 34.0 27.0 35.0 2.0 35.0 98-99 26.714079076163415 33.0 26.0 35.0 2.0 35.0 100-101 23.970959701037096 30.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3859212709516413 1101 2 0.1704981318724137 1101 3 0.27836685568415476 1101 4 0.32064278861972895 1101 5 0.2471383250063468 1101 6 0.3213351348039666 1101 7 0.43147993393963446 1101 8 0.19591211413477794 1101 9 0.4179351471058368 1101 10-11 0.4284987077932598 1101 12-13 0.3886576607339407 1101 14-15 0.608853501655453 1101 16-17 0.4405656309475674 1101 18-19 0.4831823858895845 1101 20-21 0.5299136723416424 1101 22-23 0.3922790338571289 1101 24-25 0.41599613990959483 1101 26-27 0.35350051617864864 1101 28-29 0.3148983005260817 1101 30-31 0.5859294742690722 1101 32-33 0.6517406837515409 1101 34-35 0.6354691609331766 1101 36-37 0.5353177902758617 1101 38-39 0.6869194242463905 1101 40-41 0.4991108994939992 1101 42-43 1.0054814947005823 1101 44-45 0.8235656105127234 1101 46-47 0.8382527759762652 1101 48-49 0.7832449056515287 1101 50-51 0.9348103186919658 1101 52-53 0.7364254667602665 1101 54-55 0.9873086018898931 1101 56-57 0.9579207303561148 1101 58-59 0.8957606662887656 1101 60-61 0.7868767848176219 1101 62-63 0.8259245400644559 1101 64-65 0.8273350244950635 1101 66-67 0.7947519093461892 1101 68-69 0.917400226066583 1101 70-71 1.0366623031180566 1101 72-73 0.9314374621324255 1101 74-75 1.1906446430063795 1101 76-77 1.0123295925103726 1101 78-79 0.9466607262971749 1101 80-81 0.969510732549768 1101 82-83 1.0350925991848392 1101 84-85 0.8570971710420601 1101 86-87 0.7249563569415791 1101 88-89 0.7453784737035498 1101 90-91 0.7877499556368122 1101 92-93 1.0438452764964516 1101 94-95 1.0600401727946753 1101 96-97 0.9344810565946595 1101 98-99 1.3799979517066419 1101 100-101 1.6721282667768023 1102 1 0.1629512500359951 1102 2 0.01515534776829952 1102 3 0.1270119869878954 1102 4 0.1014096954289272 1102 5 0.20832819254062684 1102 6 0.08951547410212868 1102 7 0.1231276215994086 1102 8 0.09427139835509024 1102 9 0.287644652102351 1102 10-11 0.2869044712141289 1102 12-13 0.19337529959054933 1102 14-15 0.13547678773492322 1102 16-17 0.3067194771014172 1102 18-19 0.2674398806606817 1102 20-21 0.20399222219058544 1102 22-23 0.1450654619305638 1102 24-25 0.06401844992585382 1102 26-27 -0.09296009269888117 1102 28-29 0.1245241429615902 1102 30-31 0.16712863764346508 1102 32-33 0.16515922895256097 1102 34-35 0.05419098291783797 1102 36-37 0.16161572195144203 1102 38-39 0.19272932431610457 1102 40-41 0.01348691622651188 1102 42-43 -0.31435582809746876 1102 44-45 -0.013840850096158874 1102 46-47 -0.3411290506563134 1102 48-49 -0.33663889634707544 1102 50-51 -0.2051385541874211 1102 52-53 -0.050383736121439426 1102 54-55 -0.22959123543290616 1102 56-57 -0.08418477742915087 1102 58-59 -0.14300763489644908 1102 60-61 0.11568924124337343 1102 62-63 -0.07027346132992562 1102 64-65 -0.4169085265158543 1102 66-67 -0.15869917129522548 1102 68-69 -0.29079405699175 1102 70-71 -0.5977323052946844 1102 72-73 -0.07522765522755392 1102 74-75 -0.29653174555974005 1102 76-77 -0.18043359596278918 1102 78-79 -0.03358561346577815 1102 80-81 -0.18532542826826415 1102 82-83 -0.13444493276961111 1102 84-85 -0.18505016802370733 1102 86-87 -0.010265581241082344 1102 88-89 0.32433269169100143 1102 90-91 0.41902116177206494 1102 92-93 0.32781924814414154 1102 94-95 0.4276906492064789 1102 96-97 0.5763727600573638 1102 98-99 0.5325380400171227 1102 100-101 0.45586519450164786 1103 1 -0.06251454854800897 1103 2 -0.13227483983673238 1103 3 -0.15850945949003403 1103 4 0.023649569073313614 1103 5 0.012394345581519417 1103 6 0.069597935832725 1103 7 -0.08232881050740559 1103 8 0.2229778143919674 1103 9 0.24240089412477062 1103 10-11 0.18813864232681254 1103 12-13 0.32910657352328343 1103 14-15 0.3232654726631594 1103 16-17 0.32495655916828525 1103 18-19 0.14800352220008506 1103 20-21 0.169974462615194 1103 22-23 0.11957854285876124 1103 24-25 0.35519651400358043 1103 26-27 0.31595550799533356 1103 28-29 0.1872106690086639 1103 30-31 0.46764563045331187 1103 32-33 0.5062321497323694 1103 34-35 0.4700120648377819 1103 36-37 0.41283474140983856 1103 38-39 0.5641698334116967 1103 40-41 0.5072334152760902 1103 42-43 0.9267253572210379 1103 44-45 0.6828735366291596 1103 46-47 0.7412408517303035 1103 48-49 0.6896652937740484 1103 50-51 0.3992012469126891 1103 52-53 0.45895060120029996 1103 54-55 0.5312927028952714 1103 56-57 0.4499431759955854 1103 58-59 0.3890082697491408 1103 60-61 0.2830002358346917 1103 62-63 0.2851108856337774 1103 64-65 0.5414290156103334 1103 66-67 0.4055071115907509 1103 68-69 0.6987329359099306 1103 70-71 0.8928231635342314 1103 72-73 0.32514333073190826 1103 74-75 0.531565849459497 1103 76-77 0.6667275349934165 1103 78-79 0.29830907794552886 1103 80-81 0.6812619600456742 1103 82-83 0.7729509110857045 1103 84-85 0.6545626842522552 1103 86-87 0.32656028492848677 1103 88-89 0.700056986679936 1103 90-91 0.7547221323962248 1103 92-93 0.6988511217150624 1103 94-95 0.9550600465379482 1103 96-97 1.0615654615514067 1103 98-99 1.095784718131707 1103 100-101 1.630926489830344 1104 1 0.26207511710548204 1104 2 0.11075454212882363 1104 3 0.10528993260722785 1104 4 0.16961714759408153 1104 5 0.029446017314043615 1104 6 0.04928385275267999 1104 7 0.24071070317040721 1104 8 0.31206596028862776 1104 9 0.3035761727468582 1104 10-11 0.32054998984453675 1104 12-13 0.2605807376570155 1104 14-15 0.1592381170400614 1104 16-17 0.19838614376808295 1104 18-19 0.39472084742805436 1104 20-21 0.14824700567497473 1104 22-23 0.15933031590840585 1104 24-25 0.3868935758070222 1104 26-27 0.22542359310172344 1104 28-29 0.11592394155172059 1104 30-31 0.25874998708958685 1104 32-33 0.2086637606746251 1104 34-35 -0.08837699291297696 1104 36-37 0.21775368771175607 1104 38-39 -0.040901088574116784 1104 40-41 0.12885448923758958 1104 42-43 0.3211225203416106 1104 44-45 0.42497586692297773 1104 46-47 -0.025977993254507226 1104 48-49 -0.2586781712715478 1104 50-51 -0.45903583515418944 1104 52-53 -0.18588222554741662 1104 54-55 -0.3149990904177997 1104 56-57 -0.31989978246439676 1104 58-59 -0.5262906157625125 1104 60-61 -0.5603027023618665 1104 62-63 -0.31766520352528715 1104 64-65 -0.8734342062741689 1104 66-67 -0.522299372705092 1104 68-69 -0.22631772265136618 1104 70-71 -0.36744026098451243 1104 72-73 -0.3379215122265471 1104 74-75 -0.17191945955772425 1104 76-77 -0.26677297159219293 1104 78-79 -0.546929017292566 1104 80-81 -0.4406174725784382 1104 82-83 -0.09439458030234249 1104 84-85 -0.15969770075281176 1104 86-87 -0.32145389946867553 1104 88-89 -0.7489805006554242 1104 90-91 -0.39212183923498145 1104 92-93 0.05640937906055754 1104 94-95 -0.15457521182070977 1104 96-97 -0.04115996904636532 1104 98-99 0.04345949016818196 1104 100-101 0.1282821129306484 1105 1 0.22671279826490576 1105 2 -0.09127444337842405 1105 3 -0.09673905290001983 1105 4 -0.12878864950736357 1105 5 -0.08953948993233496 1105 6 0.08696501217297481 1105 7 0.18158026838779762 1105 8 0.10322538057847908 1105 9 -0.06685860986183201 1105 10-11 -0.06213116957574982 1105 12-13 0.11920392606281638 1105 14-15 0.23880333443136692 1105 16-17 0.23969049159416755 1105 18-19 0.014358528587472108 1105 20-21 0.028174541906857087 1105 22-23 -0.04820591597565027 1105 24-25 0.11812545986499856 1105 26-27 0.13136562208722324 1105 28-29 -0.06784417439030932 1105 30-31 0.11795288564031381 1105 32-33 0.09670723893549393 1105 34-35 0.024811412884119477 1105 36-37 0.4121739775668303 1105 38-39 0.45127282446935624 1105 40-41 0.2976226051796189 1105 42-43 0.30858628845754765 1105 44-45 0.6737439828650054 1105 46-47 0.5220654850063724 1105 48-49 0.7095102345255526 1105 50-51 1.2722685126719 1105 52-53 1.2492627019888118 1105 54-55 1.1068125037851004 1105 56-57 1.4466944204341559 1105 58-59 1.401970253802702 1105 60-61 1.5785378773482819 1105 62-63 1.3041463906776087 1105 64-65 1.6938121705374272 1105 66-67 1.1344397577296803 1105 68-69 1.5171605382181994 1105 70-71 1.1008206085807046 1105 72-73 1.1271509515415694 1105 74-75 0.7132979317466237 1105 76-77 0.9887342747846155 1105 78-79 0.9965492435769967 1105 80-81 1.0871361506099682 1105 82-83 1.193141651581719 1105 84-85 0.8471863572182023 1105 86-87 0.8662997237197274 1105 88-89 0.9470339920981985 1105 90-91 1.118312943373713 1105 92-93 0.921699234133019 1105 94-95 1.0757871070198668 1105 96-97 0.7784777121130553 1105 98-99 0.3349812292986165 1105 100-101 0.3412531274234034 1106 1 0.30300104303140785 1106 2 0.18871750509178753 1106 3 0.2897343770516727 1106 4 0.19850603648297493 1106 5 0.33296453583255925 1106 6 0.18539496386379284 1106 7 0.21237736983707123 1106 8 -0.03163774341507519 1106 9 0.41079839496908477 1106 10-11 0.26536480465935597 1106 12-13 0.5762288858051718 1106 14-15 0.6493307096326575 1106 16-17 0.4178305882125315 1106 18-19 0.4448134400206456 1106 20-21 0.08695070937867655 1106 22-23 0.21034883442692376 1106 24-25 0.5842083906218392 1106 26-27 0.5663495190276464 1106 28-29 0.669072089699867 1106 30-31 0.507546283385885 1106 32-33 0.6744045014153599 1106 34-35 0.6747711552351703 1106 36-37 0.7064573914154622 1106 38-39 0.8106729854999486 1106 40-41 1.1995952299783283 1106 42-43 0.8726965944156788 1106 44-45 0.6613647558118672 1106 46-47 0.5673553400788265 1106 48-49 0.31548849539511536 1106 50-51 0.3068900907717378 1106 52-53 0.7636548114896158 1106 54-55 1.0193527614340496 1106 56-57 0.8688965138319062 1106 58-59 0.8426908657189642 1106 60-61 0.38006766800850755 1106 62-63 0.3486310927710079 1106 64-65 0.04304727520731433 1106 66-67 -0.005354928260651093 1106 68-69 -0.07261401894766806 1106 70-71 0.1271893686451122 1106 72-73 0.4780970062919643 1106 74-75 0.5531731330348677 1106 76-77 0.9523011024818793 1106 78-79 0.8789969086333578 1106 80-81 0.603641786680825 1106 82-83 0.8072720863643248 1106 84-85 0.6916911881360797 1106 86-87 1.0101201746053974 1106 88-89 1.1889824623075391 1106 90-91 1.00408186446872 1106 92-93 0.8995575272087031 1106 94-95 1.2008877511422504 1106 96-97 1.3941178087314121 1106 98-99 1.3645706012792935 1106 100-101 1.7068932240417602 1107 1 0.14183889663303972 1107 2 -0.05541343687379907 1107 3 -0.11730849258962195 1107 4 -0.008624322222189562 1107 5 0.15598145038490685 1107 6 0.058863905246120396 1107 7 -0.04385622596345229 1107 8 0.01655414926500498 1107 9 -0.009914640901435234 1107 10-11 0.022072299555830455 1107 12-13 0.1687303439562342 1107 14-15 -0.013635898707967442 1107 16-17 0.01931790229826902 1107 18-19 0.31826415451466517 1107 20-21 0.12471682194793487 1107 22-23 0.130970735855918 1107 24-25 0.23018753906161749 1107 26-27 0.20538422302298187 1107 28-29 0.26337800979318615 1107 30-31 0.22814631254890827 1107 32-33 -0.22480080625451393 1107 34-35 -0.1219990401570783 1107 36-37 0.023094895060843612 1107 38-39 -0.1690769415924933 1107 40-41 0.16423506666541243 1107 42-43 0.19117501377992596 1107 44-45 0.48687875406209 1107 46-47 -0.0445213003411169 1107 48-49 -0.7424183287518602 1107 50-51 -0.43793347294946017 1107 52-53 -0.7650160838151407 1107 54-55 -0.6213770431737018 1107 56-57 -0.8714614622544232 1107 58-59 -0.8879704057887636 1107 60-61 -0.6609063769025454 1107 62-63 -1.1727308203231885 1107 64-65 -0.9846940487938554 1107 66-67 -1.0710264068258368 1107 68-69 -0.9400316334125236 1107 70-71 -0.819697478832282 1107 72-73 -0.7964910660323241 1107 74-75 -0.8473262837047066 1107 76-77 -0.707730976841539 1107 78-79 -0.770026130153461 1107 80-81 -0.906273640557437 1107 82-83 -0.690431325709163 1107 84-85 -0.5927029500966441 1107 86-87 -0.8118213535369172 1107 88-89 -0.80317997572104 1107 90-91 -0.56166252164968 1107 92-93 -0.9372389149026965 1107 94-95 -0.9387484401671706 1107 96-97 -0.9307400215398012 1107 98-99 -0.6195720058948098 1107 100-101 -0.014946233526043073 1108 1 0.01713535806933919 1108 2 -0.06792015666636075 1108 3 -0.183826533256223 1108 4 0.10178582229288224 1108 5 -0.048505789914877084 1108 6 -0.02159968138386148 1108 7 0.0572568879093609 1108 8 0.07869246631272375 1108 9 0.22221071089947486 1108 10-11 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-1.0019053915705882 2316 54-55 -0.7608292062479194 2316 56-57 -1.046688714253328 2316 58-59 -0.8410911305630293 2316 60-61 -0.28148674354590497 2316 62-63 -0.16806417392425743 2316 64-65 0.0543264114864499 2316 66-67 -0.062331547737272786 2316 68-69 -0.009104080437722928 2316 70-71 -0.958199591743842 2316 72-73 -0.9092278185328553 2316 74-75 -1.0378911455294109 2316 76-77 -1.1909492907685149 2316 78-79 -0.42562914599269774 2316 80-81 -0.29203960900057524 2316 82-83 -0.16071375662151866 2316 84-85 -0.26854583460094616 2316 86-87 -0.4181205661353431 2316 88-89 -0.5653254170003414 2316 90-91 -0.3703908225039676 2316 92-93 -1.245533992882816 2316 94-95 -1.4075867948322944 2316 96-97 -1.4868613847477796 2316 98-99 -1.5713474596387655 2316 100-101 -1.0463510917025545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 5.0 6 16.0 7 27.0 8 75.0 9 120.0 10 241.0 11 444.0 12 763.0 13 1039.0 14 1316.0 15 1974.0 16 2817.0 17 4313.0 18 3576.0 19 1818.0 20 1239.0 21 1205.0 22 1220.0 23 1369.0 24 1512.0 25 1647.0 26 1985.0 27 2427.0 28 2927.0 29 3657.0 30 4829.0 31 5980.0 32 7788.0 33 10455.0 34 14629.0 35 21621.0 36 34207.0 37 56838.0 38 53230.0 39 5029.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.57579937453179 22.442338410803398 16.45560087359438 19.52626134107043 2 14.268107096999191 24.23592727042151 40.40430860929252 21.091657023286782 3 18.910821639315678 28.79284448971019 33.06451293310137 19.231820937872765 4 11.4405621009832 19.964809244706526 41.58453508970076 27.010093564609512 5 11.072538203030879 40.31489759685499 36.34558683659882 12.266977363515313 6 25.534005976111406 41.19276525929507 18.67415926257639 14.599069502017137 7 22.998426719611317 37.707607623078474 23.44068891451579 15.853276742794414 8 22.544672048315956 35.33777973281974 22.06278062447739 20.054767594386917 9 24.468274820776813 19.335893381522474 23.88096964797356 32.314862149727155 10-11 21.05326773361972 28.771669800684396 29.665310683863144 20.509751781832737 12-13 25.315597897268947 27.160342159440592 29.07087107199258 18.45318887129788 14-15 22.720823970928 28.4411050214196 29.390026908246448 19.44804409940596 16-17 18.000784658732897 31.515540602126503 31.245665553085335 19.238009186055265 18-19 17.35641339626455 30.819453195899165 34.098970036340006 17.72516337149628 20-21 18.942627401093002 29.965165079478307 32.82844495171064 18.263762567718057 22-23 18.714110779546562 29.043271155073136 32.24372768379046 19.998890381589845 24-25 19.049006144840693 29.24615229138553 33.45817208328132 18.246669480492457 26-27 20.03939239021452 28.206774381472105 34.001894322185365 17.751938906128014 28-29 18.48614609072661 28.308223969056336 35.3692295193388 17.836400420878256 30-31 18.637682423166254 29.87657452694829 34.0862789241294 17.39946412575605 32-33 18.146367754233424 30.25458018443589 33.29812908927347 18.300922972057208 34-35 18.97786064870499 28.52237217808971 33.35308232953611 19.146684843669192 36-37 19.999247001381157 28.545979482769212 33.32771886994723 18.127054645902398 38-39 19.949946796178835 28.279465702855173 34.22541379098527 17.54517370998072 40-41 18.443131333547342 28.901980366746326 35.27842838855929 17.376459911147045 42-43 18.12568733714444 30.0759910039333 33.542647102533365 18.255674556388893 44-45 18.956877369089536 30.067350008024935 32.049409820538685 18.926362802346848 46-47 19.167903431303586 29.873760841092423 30.464039375679892 20.494296351924103 48-49 19.77359821509953 29.668025949219107 31.117464997483545 19.440910838197823 50-51 20.786323160510268 30.146548888598275 30.819651342043837 18.24747660884762 52-53 19.379440985665994 30.525051815627513 31.83461006511134 18.26089713359515 54-55 18.750767840653744 29.855229836999815 31.965751334556597 19.42825098778985 56-57 20.12007727745579 31.519294595531775 29.42765145885966 18.932976668152772 58-59 19.301203959831017 30.072047365792958 30.56762069320821 20.05912798116782 60-61 19.311712732799258 29.95028463975683 30.74584929251218 19.99215333493173 62-63 21.430694422978615 29.572123215198605 30.371841055088588 18.625341306734196 64-65 21.020531903510754 29.515849710112192 31.12875932772976 18.334859058647297 66-67 19.26495706173045 30.476462219474598 31.07704318397077 19.181537534824187 68-69 19.22631856351971 30.85928057097793 31.031865862985903 18.882535002516455 70-71 19.44534711379182 30.977894728499077 30.138148039534272 19.43861011817483 72-73 19.359869065027603 29.606402498226593 30.12237505894848 20.91135337779733 74-75 19.633982991067537 30.56654170200287 29.954267688576437 19.84520761835316 76-77 20.601895884851942 29.48826168283559 30.652582271257373 19.2572601610551 78-79 19.331137874050384 29.209317624306983 31.295598381542288 20.16394612010034 80-81 19.244350562367732 31.405602276420186 29.79736531312667 19.552681848085413 82-83 19.08660743300546 31.430564252142002 29.480965704185724 20.001862610666816 84-85 19.062735306939327 29.540086394800458 29.91340704632822 21.483771251931994 86-87 19.46415514573149 30.601334361085957 29.508323409179628 20.426187084002926 88-89 20.612042300825685 28.59268711695342 30.555351902526596 20.239918679694295 90-91 19.0553184406691 28.774783129044657 31.71487562366499 20.45502280662125 92-93 18.852813080815885 30.115638090029684 30.668862125949616 20.362686703204815 94-95 18.57521033213257 30.099588252311378 30.069668184466135 21.255533231089924 96-97 18.893025781050305 29.72102727905 29.06793111579181 22.31801582410789 98-99 19.235883097762656 29.402311650791326 29.483554306752453 21.878250944693566 100-101 18.871749145489673 30.59592807252192 29.58299895972656 20.94932382226185 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 893.0 1 566.5 2 231.0 3 188.0 4 146.0 5 150.0 6 183.0 7 295.0 8 422.5 9 475.5 10 459.0 11 399.0 12 385.5 13 423.5 14 471.0 15 479.5 16 513.5 17 566.5 18 603.0 19 676.0 20 758.0 21 889.5 22 1073.5 23 1253.5 24 1453.0 25 1794.0 26 2397.0 27 3095.0 28 3510.5 29 3976.5 30 4776.0 31 5482.0 32 6682.5 33 7985.5 34 9251.0 35 10476.0 36 11790.0 37 13079.5 38 12973.5 39 11947.0 40 10856.5 41 9472.5 42 8020.5 43 7901.0 44 8275.0 45 7824.5 46 7311.5 47 8473.0 48 18337.5 49 17807.0 50 7060.5 51 4476.5 52 3148.0 53 2421.0 54 1898.0 55 1539.0 56 1203.0 57 944.5 58 757.0 59 581.5 60 425.5 61 307.0 62 198.0 63 123.0 64 78.0 65 54.0 66 44.5 67 27.0 68 17.0 69 12.0 70 7.0 71 4.0 72 2.5 73 0.5 74 0.5 75 1.5 76 1.5 77 0.5 78 0.5 79 0.5 80 0.0 81 0.0 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01902202988836446 2 0.002774046025386484 3 7.925845786818526E-4 4 0.0 5 0.0011888768680227788 6 3.962922893409263E-4 7 0.0 8 0.0 9 0.0 10-11 1.9814614467046315E-4 12-13 1.9814614467046315E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0023777537360455576 22-23 0.0 24-25 0.005349945906102505 26-27 0.0023777537360455576 28-29 0.0037647767487387993 30-31 0.016247983862977978 32-33 7.925845786818526E-4 34-35 0.0021796075913750944 36-37 0.005746238195443431 38-39 0.0029721921700569475 40-41 0.004755507472091115 42-43 0.002575899880716021 44-45 1.9814614467046315E-4 46-47 1.9814614467046315E-4 48-49 0.0 50-51 0.0 52-53 7.925845786818526E-4 54-55 0.0031703383147274103 56-57 5.944384340113894E-4 58-59 0.0019814614467046315 60-61 9.907307233523158E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 3.962922893409263E-4 72-73 0.0 74-75 3.962922893409263E-4 76-77 0.0011888768680227788 78-79 0.0 80-81 0.005151799761432042 82-83 0.0019814614467046315 84-85 0.003566630604068337 86-87 0.0033684844593978736 88-89 0.001387023012693242 90-91 0.0 92-93 0.0 94-95 0.0 96-97 9.907307233523158E-4 98-99 0.0033684844593978736 100-101 5.944384340113894E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 252339.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.98672079723419 #Duplication Level Percentage of deduplicated Percentage of total 1 78.43973249274778 32.9342714758274 2 11.57781754208146 9.72229185161389 3 3.4289937765210867 4.319166129307336 4 1.6444203982347638 2.7617528053583875 5 0.8946877305287704 1.8782501971211292 6 0.5694938697082954 1.4346708061907198 7 0.4059228495631049 1.1930358544876647 8 0.3550573583552438 1.1926155337812117 9 0.27427178263419083 1.0364195484019325 >10 2.0355952358782017 17.243508178603324 >50 0.24335386441143397 6.93207621313761 >100 0.11070606126913594 8.123016454176838 >500 0.007978815226604393 2.7646228802183463 >1k 0.01196822283990659 8.464302071774226 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3296 1.306179385667693 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3286 1.3022164627742838 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2080 0.8242879618291267 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1941 0.7692033336107379 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1880 0.7450295039609415 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1253 0.4965542385441807 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1134 0.44939545611261045 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1123 0.4450362409298602 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1100 0.43592151827501896 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1059 0.4196735344120409 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1053 0.4172957806759954 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.39946262765565377 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 986 0.3907441972901533 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 982 0.38915902813278963 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 962 0.3812331823459711 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 926 0.36696665992969774 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 776 0.3075228165285588 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 774 0.306730231949877 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 665 0.263534372411716 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 531 0.21043120564003184 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 479 0.18982400659430368 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 477 0.18903142201562184 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 430 0.1704056844165983 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 413 0.16366871549780257 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 392 0.15534657742164312 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 365 0.14464668560943808 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 333 0.13196533235052846 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 325 0.12879499403580105 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 308 0.12205802511700531 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 298 0.11809510222359604 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 293 0.1161136407768914 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 279 0.11056554872611844 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 272 0.10779150270073196 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 272 0.10779150270073196 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 265 0.10501745667534546 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 264 0.10462116438600454 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9814614467046315E-4 0.0 22-23 0.0 0.0 0.0 0.0031703383147274103 0.0 24-25 0.0 0.0 0.0 0.004755507472091115 0.0 26-27 0.0 0.0 0.0 0.010699891812205011 0.0 28-29 0.0 0.0 0.0 0.023975683505126043 0.0 30-31 0.0 0.0 0.0 0.049932828456956714 0.0 32-33 0.0 0.0 0.0 0.08163621160423082 0.0 34-35 0.0 0.0 0.0 0.10977296414743659 0.0 36-37 0.0 0.0 0.0 0.13077645548250566 0.0 38-39 0.0 0.0 0.0 0.14563741633279043 0.0 40-41 0.0 0.0 0.0 0.16386686164247302 0.0 42-43 0.0 0.0 0.0 0.1817000146628147 0.0 44-45 0.0 0.0 0.0 0.21340339781008882 0.0 46-47 0.0 0.0 0.0 0.24708824240406757 0.0 48-49 0.0 0.0 0.0 0.2833489868787623 0.0 50-51 0.0 0.0 0.0 0.314457931592025 0.0 52-53 0.0 0.0 0.0 0.400453358379006 0.0 54-55 0.0 0.0 0.0 0.4993282845695671 0.0 56-57 0.0 0.0 0.0 0.6251510864353113 0.0 58-59 0.0 0.0 0.0 0.7987271091666369 0.0 60-61 0.0 0.0 0.0 1.0495801283194433 0.0 62-63 0.0 0.0 0.0 1.2100785055025185 0.0 64-65 0.0 0.0 0.0 1.3574992371373429 0.0 66-67 0.0 0.0 0.0 1.6689849765593112 0.0 68-69 0.0 0.0 0.0 2.042688605407805 0.0 70-71 0.0 0.0 0.0 2.319300623367771 0.0 72-73 0.0 0.0 0.0 2.559651896853043 0.0 74-75 0.0 0.0 0.0 3.1114889097602827 0.0 76-77 0.0 0.0 0.0 3.4360522947305014 0.0 78-79 0.0 0.0 0.0 3.637368777715692 0.0 80-81 0.0 0.0 0.0 3.864642405652713 0.0 82-83 0.0 0.0 0.0 4.266879079333753 0.0 84-85 0.0 0.0 0.0 4.820301261398357 0.0 86-87 0.0 0.0 0.0 5.601195217544652 0.0 88-89 0.0 0.0 0.0 6.644632815379311 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATGGT 25 0.0050170785 56.999046 9 GATTGGC 40 5.3805823E-4 47.499207 9 CGCAGAA 55 4.9527443E-5 43.181095 9 CATTCCG 60 8.273169E-5 39.582672 9 CGATTCC 25 0.0016150896 37.999367 38-39 ACTATGT 75 3.071992E-4 31.66614 3 GGGGTCC 80 4.4842533E-4 29.687004 6 GGATGAC 65 0.0058360207 29.23028 8 TAATACC 165 2.6266207E-9 28.787397 4 ATAAGAC 85 6.393843E-4 27.94071 3 ATACCTT 170 3.612513E-9 27.94071 6 CATGGGT 105 6.885629E-5 27.142405 4 TTAGGCA 105 6.885629E-5 27.142405 4 GGGTCCT 90 8.928997E-4 26.388449 7 GTAGATA 90 8.928997E-4 26.388449 4 AATACCT 200 7.1122486E-10 26.124563 5 TATAGGG 110 9.448991E-5 25.908657 2 GTTATAG 345 0.0 24.787107 1 ATGGGTA 100 0.0016502434 23.749603 5 ATATGAG 100 0.0016502434 23.749603 3 >>END_MODULE