##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936946_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 213021 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.348871707484236 33.0 31.0 34.0 31.0 34.0 2 32.44083447171875 34.0 31.0 34.0 31.0 34.0 3 32.552189690218334 34.0 31.0 34.0 31.0 34.0 4 36.1808225480117 37.0 35.0 37.0 35.0 37.0 5 36.102722266818766 37.0 35.0 37.0 35.0 37.0 6 36.171062946845616 37.0 36.0 37.0 35.0 37.0 7 36.1418123095845 37.0 36.0 37.0 35.0 37.0 8 36.14630013003413 37.0 36.0 37.0 35.0 37.0 9 37.8517141502481 39.0 38.0 39.0 35.0 39.0 10-11 37.77608545636346 39.0 37.0 39.0 35.0 39.0 12-13 37.72514916369748 39.0 37.0 39.0 35.0 39.0 14-15 38.90504692025669 40.0 38.0 41.0 36.0 41.0 16-17 38.849676792428916 40.0 38.0 41.0 35.5 41.0 18-19 38.9628581219692 40.0 38.5 41.0 35.5 41.0 20-21 39.075152215039836 40.0 38.5 41.0 35.5 41.0 22-23 39.03633679308613 40.0 38.5 41.0 35.0 41.0 24-25 38.958102722266815 40.0 38.0 41.0 35.0 41.0 26-27 38.72328549767394 40.0 38.0 41.0 35.0 41.0 28-29 38.551980790626274 40.0 38.0 41.0 34.5 41.0 30-31 38.476232390233825 40.0 38.0 41.0 35.0 41.0 32-33 38.337375188361705 40.0 38.0 41.0 34.5 41.0 34-35 38.20255045277226 40.0 38.0 41.0 34.0 41.0 36-37 38.001809680735704 40.0 37.0 41.0 34.0 41.0 38-39 37.79655996357167 40.0 37.0 41.0 33.5 41.0 40-41 37.552173259913346 40.0 37.0 41.0 33.0 41.0 42-43 37.32255505325766 40.0 37.0 41.0 33.0 41.0 44-45 37.014115979175756 40.0 37.0 41.0 32.5 41.0 46-47 36.487212528342276 40.0 37.0 41.0 31.0 41.0 48-49 36.00362875021712 40.0 36.0 41.0 29.5 41.0 50-51 35.740194628698575 40.0 36.0 41.0 28.0 41.0 52-53 35.46166575126396 39.0 36.0 41.0 27.0 41.0 54-55 35.235009693879945 39.0 35.0 40.5 26.0 41.0 56-57 35.0730514831871 39.0 35.0 40.0 26.0 41.0 58-59 34.803906657090145 39.0 35.0 40.0 24.5 41.0 60-61 34.48669145295534 38.5 35.0 40.0 22.5 41.0 62-63 34.13088380957746 38.0 34.0 40.0 22.0 41.0 64-65 33.661732880795796 38.0 34.0 40.0 19.5 41.0 66-67 33.241790715469364 37.0 33.0 39.5 18.0 41.0 68-69 32.823484539083 36.5 33.0 39.0 13.5 40.0 70-71 32.41456006684787 36.0 33.0 39.0 10.0 40.0 72-73 32.02101436008657 35.5 32.0 38.5 7.5 40.0 74-75 31.525481055858343 35.0 31.5 37.5 4.0 39.5 76-77 29.988125583862626 34.0 29.5 36.0 2.0 38.5 78-79 30.576436126015746 34.5 31.0 36.0 2.0 39.0 80-81 30.231634439797013 35.0 31.0 36.0 2.0 38.5 82-83 30.084961107120893 34.0 31.0 36.0 2.0 37.0 84-85 29.789584125508753 34.0 31.0 35.0 2.0 37.0 86-87 29.4399636655541 34.0 30.0 35.0 2.0 36.5 88-89 28.999370954037396 34.0 29.5 35.0 2.0 36.0 90-91 28.54653297092775 34.0 29.0 35.0 2.0 36.0 92-93 28.259601166082216 34.0 29.0 35.0 2.0 35.0 94-95 27.904492984259768 34.0 29.0 35.0 2.0 35.0 96-97 27.325946737645584 34.0 27.0 35.0 2.0 35.0 98-99 26.70449392313434 33.0 26.0 35.0 2.0 35.0 100-101 23.831075339989955 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36810857073941605 1101 2 0.2329916530817755 1101 3 0.18302514077306853 1101 4 0.15810180828292175 1101 5 -0.009017992091287397 1101 6 0.18028888098082518 1101 7 0.18332738170369822 1101 8 0.13992081504719067 1101 9 0.35789315071303207 1101 10-11 0.22417285255322383 1101 12-13 0.24479715562872428 1101 14-15 0.2460737368421917 1101 16-17 0.41527800077810184 1101 18-19 0.3985761820494673 1101 20-21 0.21039124719936098 1101 22-23 0.18298992496082178 1101 24-25 0.04530036499161838 1101 26-27 0.48082558280212595 1101 28-29 0.5626854687309404 1101 30-31 0.39622632085274034 1101 32-33 0.40053236897924904 1101 34-35 0.566478185706039 1101 36-37 0.5211797533807854 1101 38-39 0.6548136571233414 1101 40-41 0.7694636704549893 1101 42-43 0.7597636310017606 1101 44-45 0.6483728199700778 1101 46-47 0.6143962246977921 1101 48-49 0.7768031614477948 1101 50-51 0.7583710717359082 1101 52-53 0.9998426647661844 1101 54-55 0.934779842684911 1101 56-57 1.173798907161384 1101 58-59 1.168131408841262 1101 60-61 0.9535894927190469 1101 62-63 1.01682024714124 1101 64-65 1.298279024476031 1101 66-67 1.338461754586561 1101 68-69 1.3751139409684896 1101 70-71 1.1104959989024152 1101 72-73 1.1734766100564116 1101 74-75 1.203492584953537 1101 76-77 1.2704336706297354 1101 78-79 1.5947102983329629 1101 80-81 1.7394945424458967 1101 82-83 1.4099929311623605 1101 84-85 1.334738042295573 1101 86-87 1.5012308406416608 1101 88-89 1.351391122202017 1101 90-91 1.035057467860181 1101 92-93 1.24704482975541 1101 94-95 1.1663396370896102 1101 96-97 1.2540731156286604 1101 98-99 1.203045643695937 1101 100-101 1.7725417327502235 1102 1 0.15896381254402314 1102 2 -0.06979153419508322 1102 3 -0.0067265632191393365 1102 4 0.024541275988212874 1102 5 0.04940459797686714 1102 6 0.10454237010773682 1102 7 0.027087524514477934 1102 8 0.13046771540422242 1102 9 0.08472697090128634 1102 10-11 0.1437809343448464 1102 12-13 0.05173889532361642 1102 14-15 -0.1100403494934099 1102 16-17 -0.08859655293821334 1102 18-19 -0.004519403799285726 1102 20-21 -0.16324961615669054 1102 22-23 0.02798749068623607 1102 24-25 -0.163479252015847 1102 26-27 -0.09228379726927471 1102 28-29 -0.2698161541187787 1102 30-31 -0.11117793101353612 1102 32-33 0.0547842352564345 1102 34-35 0.11214111981833952 1102 36-37 -0.06896954879383799 1102 38-39 -0.16868114975753912 1102 40-41 0.1666561404322664 1102 42-43 0.24850511104070705 1102 44-45 0.41196418640954846 1102 46-47 0.38723783456472205 1102 48-49 0.3257929374945334 1102 50-51 0.26536961117115254 1102 52-53 0.23028732559058795 1102 54-55 0.0125143444700484 1102 56-57 0.19477423984557873 1102 58-59 -0.1301686560727262 1102 60-61 -0.09314857933548382 1102 62-63 0.05601206797862801 1102 64-65 0.5695383558619511 1102 66-67 0.32030612329801045 1102 68-69 0.264090734217433 1102 70-71 0.30933566134967094 1102 72-73 0.08896671067309825 1102 74-75 0.5693843408769368 1102 76-77 0.5163968319410017 1102 78-79 0.17315479687239943 1102 80-81 0.41068490271853264 1102 82-83 0.38382447936099595 1102 84-85 0.5407791003935962 1102 86-87 0.08511188575688067 1102 88-89 0.44381241399039695 1102 90-91 0.1427335470552471 1102 92-93 0.2412431419916956 1102 94-95 0.677456157699801 1102 96-97 0.7610310838208072 1102 98-99 1.0291167245138553 1102 100-101 1.0172268999037435 1103 1 -0.11282411673487758 1103 2 -0.04973923390812729 1103 3 -0.09395927635438284 1103 4 -0.022552123993271778 1103 5 -0.0912507755304901 1103 6 -0.27700673662187825 1103 7 -0.018050400136083056 1103 8 0.01083659441930962 1103 9 -0.044531105558959894 1103 10-11 -0.18847052358350425 1103 12-13 -0.19124337811894065 1103 14-15 -0.28524052537048306 1103 16-17 -0.1236620440055134 1103 18-19 0.03407519955163707 1103 20-21 -0.03506184424148273 1103 22-23 0.08094153123052905 1103 24-25 -0.08053932792068963 1103 26-27 -0.1573467427473858 1103 28-29 -0.03828903202306577 1103 30-31 -0.17380521047294195 1103 32-33 -0.02506626923305788 1103 34-35 -0.09857002523396119 1103 36-37 -0.0446476011866892 1103 38-39 -0.031815232882593136 1103 40-41 0.020400470710896457 1103 42-43 0.23585237568941864 1103 44-45 0.3896902810128964 1103 46-47 0.5001694276407136 1103 48-49 0.5756367308852575 1103 50-51 0.9083333712378021 1103 52-53 0.8040399868883767 1103 54-55 0.9513623496537988 1103 56-57 0.8053222357726355 1103 58-59 0.888605064190159 1103 60-61 0.5797547808422436 1103 62-63 0.7310676081063718 1103 64-65 0.3223616456888436 1103 66-67 0.6742760853455962 1103 68-69 0.8142561975146663 1103 70-71 1.0278976348755506 1103 72-73 0.9222884731464873 1103 74-75 0.8015710019895934 1103 76-77 0.8316969800480081 1103 78-79 1.1809130584663983 1103 80-81 1.3762673798082297 1103 82-83 1.13434516805858 1103 84-85 1.2806835250904385 1103 86-87 0.9400075166001685 1103 88-89 1.4390105071126804 1103 90-91 1.0681824964211621 1103 92-93 0.9410024681048128 1103 94-95 1.1769586107221102 1103 96-97 1.4631566507929428 1103 98-99 1.876902061920127 1103 100-101 1.5201311857502695 1104 1 0.13630167076423305 1104 2 0.20792339793161574 1104 3 0.09934432667527915 1104 4 0.03758803315261616 1104 5 0.11049057082755098 1104 6 0.038516729082097356 1104 7 0.1484676150122155 1104 8 0.14392925386575683 1104 9 -0.007693843578351789 1104 10-11 0.04866404143383818 1104 12-13 0.13667479275951422 1104 14-15 0.5303254121958787 1104 16-17 0.5390618181025033 1104 18-19 0.5214044272716123 1104 20-21 0.24604525563817958 1104 22-23 0.28396411210403727 1104 24-25 0.23697323667193615 1104 26-27 0.11227879699919185 1104 28-29 -0.015913439186654443 1104 30-31 0.28699326054000807 1104 32-33 0.13016130916292212 1104 34-35 0.2993771680838009 1104 36-37 0.43909983280496334 1104 38-39 -0.02778748459917324 1104 40-41 -0.07331369365520857 1104 42-43 -0.08805220419306892 1104 44-45 0.18628545337787727 1104 46-47 0.25480947860695125 1104 48-49 0.542855531763017 1104 50-51 0.6585634271590948 1104 52-53 0.8182963753643477 1104 54-55 0.9141357622679323 1104 56-57 0.5568406048531145 1104 58-59 0.5590658838971052 1104 60-61 0.7496493090506391 1104 62-63 0.2221131237855687 1104 64-65 0.6929923528453585 1104 66-67 0.4496990343085727 1104 68-69 0.5883554400997895 1104 70-71 0.7117866417418313 1104 72-73 0.8691703305826053 1104 74-75 0.5026611131243008 1104 76-77 0.7156992452140045 1104 78-79 0.8632943142177965 1104 80-81 1.4253719313761515 1104 82-83 1.1285831520608482 1104 84-85 1.0743198243754968 1104 86-87 1.1119406942033372 1104 88-89 0.7969421274142299 1104 90-91 1.0103428984881297 1104 92-93 1.1079098086583627 1104 94-95 1.6242510294946726 1104 96-97 1.388802577033477 1104 98-99 0.9665601634278786 1104 100-101 0.7959222241873043 1105 1 0.17919706680811487 1105 2 -0.17084484945544887 1105 3 0.14445100370732433 1105 4 0.16062508938480136 1105 5 0.31727989448652494 1105 6 0.31869294053583985 1105 7 0.32818602219887794 1105 8 0.27373835812419856 1105 9 0.2926084799942501 1105 10-11 0.22271604108832577 1105 12-13 0.19981723035129306 1105 14-15 0.4871795194775572 1105 16-17 0.3981099307396292 1105 18-19 0.46668386063085165 1105 20-21 0.6247530383107218 1105 22-23 0.3645894900084983 1105 24-25 0.5140311964197153 1105 26-27 0.6453616336580907 1105 28-29 0.5308516985865168 1105 30-31 0.5238680985809552 1105 32-33 0.7350762266717723 1105 34-35 0.646289148735093 1105 36-37 0.6056887591243836 1105 38-39 0.8527169638762615 1105 40-41 1.1133408800685487 1105 42-43 0.7519149721047569 1105 44-45 1.0485986605990902 1105 46-47 1.071186778425556 1105 48-49 0.888420013062138 1105 50-51 0.5285642909407215 1105 52-53 0.44256000151965225 1105 54-55 0.27622438626378454 1105 56-57 0.7596392573537614 1105 58-59 0.759325018387905 1105 60-61 0.8717362054832236 1105 62-63 1.018778926670599 1105 64-65 0.8955577843042875 1105 66-67 0.6653939466347367 1105 68-69 0.9341531424406426 1105 70-71 1.0029847068709685 1105 72-73 1.1220769557877475 1105 74-75 0.6766008211661045 1105 76-77 0.6789194231530189 1105 78-79 1.027801526794459 1105 80-81 1.3662719918408648 1105 82-83 1.249590321448423 1105 84-85 0.6985575370817223 1105 86-87 0.6198529339891223 1105 88-89 0.56488719090218 1105 90-91 0.8009708677375009 1105 92-93 1.0437394709197463 1105 94-95 1.3251320867633964 1105 96-97 0.985580671531185 1105 98-99 1.4886643354931834 1105 100-101 2.1485524392689292 1106 1 0.21293124151145548 1106 2 0.028008188604637496 1106 3 0.15482922333664817 1106 4 0.2537512551981891 1106 5 0.055641604213874984 1106 6 0.17635456691993312 1106 7 0.1673335397604916 1106 8 0.07546078539728995 1106 9 0.3810713922457083 1106 10-11 0.10285058091449173 1106 12-13 0.13667479275951422 1106 14-15 0.29211661516355036 1106 16-17 0.10938508254341883 1106 18-19 0.3445363827565018 1106 20-21 0.13928849888142736 1106 22-23 0.17182844702718825 1106 24-25 0.19047085193425772 1106 26-27 0.28583470584922566 1106 28-29 0.5248390780364502 1106 30-31 0.5244336420980389 1106 32-33 0.6470399525121522 1106 34-35 0.557697252874469 1106 36-37 0.6541766743523922 1106 38-39 1.028174359597969 1106 40-41 0.8425050662811984 1106 42-43 0.6322975573331533 1106 44-45 0.3900480394934007 1106 46-47 0.46675966408654546 1106 48-49 1.0411862153878175 1106 50-51 0.9782507615523031 1106 52-53 1.117501462804725 1106 54-55 1.325317532273239 1106 56-57 1.1334436785150146 1106 58-59 1.367968904564833 1106 60-61 0.9854203742334704 1106 62-63 0.8956690326355883 1106 64-65 0.8269881133117067 1106 66-67 1.209555950047843 1106 68-69 1.202716860343564 1106 70-71 1.2068581838721926 1106 72-73 0.7787622754686652 1106 74-75 1.0952419292345255 1106 76-77 0.8195413225854935 1106 78-79 1.2125258987434968 1106 80-81 0.9418266213602529 1106 82-83 0.9488799194164343 1106 84-85 0.5946165917310857 1106 86-87 0.5114372495822472 1106 88-89 0.4544672996452839 1106 90-91 0.8243863537080536 1106 92-93 0.5407926915412489 1106 94-95 1.1719987772424219 1106 96-97 0.9163065515962749 1106 98-99 1.0127445831417106 1106 100-101 1.2389004965773402 1107 1 0.2615883892252029 1107 2 0.3642312040729365 1107 3 0.12045050499565946 1107 4 0.047521440477808596 1107 5 0.19817092598847807 1107 6 0.2300261630443572 1107 7 0.14362114812357163 1107 8 0.2640550400292767 1107 9 -0.019107088121451454 1107 10-11 0.09379907657996966 1107 12-13 0.4755112707469422 1107 14-15 0.46636869743449694 1107 16-17 0.22928564348171676 1107 18-19 0.18908848202558914 1107 20-21 0.10202009840547532 1107 22-23 0.1670477229068439 1107 24-25 0.2900161519512565 1107 26-27 0.4522251529000485 1107 28-29 0.27375544723584255 1107 30-31 0.3015881551016051 1107 32-33 0.3146415163401244 1107 34-35 0.3726938532484283 1107 36-37 0.7509385305482041 1107 38-39 0.8779746315680512 1107 40-41 0.8426962952460144 1107 42-43 0.8171734709826524 1107 44-45 0.9514537848614069 1107 46-47 1.1818072588511228 1107 48-49 1.4414496864059956 1107 50-51 1.1865415995601296 1107 52-53 1.3341213846133329 1107 54-55 1.3856396468406302 1107 56-57 1.568383335152781 1107 58-59 1.6619515664720197 1107 60-61 1.6840647733495686 1107 62-63 1.6980657689945886 1107 64-65 2.092881365054012 1107 66-67 2.1343179762249633 1107 68-69 2.652848595852653 1107 70-71 2.3557193091484194 1107 72-73 2.7699657430872264 1107 74-75 2.576671471984824 1107 76-77 2.0876655789172958 1107 78-79 2.509058280181538 1107 80-81 2.4574011581612005 1107 82-83 2.1751887754422725 1107 84-85 2.1149506049896267 1107 86-87 2.541056491465639 1107 88-89 2.048190740066836 1107 90-91 2.030191215173293 1107 92-93 1.7514632677778614 1107 94-95 1.9879302747776926 1107 96-97 2.0250308419243375 1107 98-99 2.4605390757475263 1107 100-101 1.2342685060962957 1108 1 0.07639970997992407 1108 2 0.14008026067671153 1108 3 -0.12634194783452557 1108 4 0.050823327270265395 1108 5 0.08901998259225508 1108 6 0.05851672908209338 1108 7 0.005036242463191343 1108 8 0.07726258719909396 1108 9 -0.03014482397050955 1108 10-11 0.09974247280639048 1108 12-13 0.11584145942618562 1108 14-15 -0.027027528980589466 1108 16-17 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-0.11600990911491138 2316 88-89 -0.030546560368577502 2316 90-91 -0.1665613247396216 2316 92-93 0.15390339840195466 2316 94-95 -0.015652816659173396 2316 96-97 -0.2261484033586818 2316 98-99 -0.2954025062553782 2316 100-101 -1.2624205359936944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 3.0 6 9.0 7 24.0 8 62.0 9 91.0 10 178.0 11 376.0 12 625.0 13 948.0 14 1139.0 15 1656.0 16 2475.0 17 3591.0 18 3325.0 19 1510.0 20 1013.0 21 996.0 22 1089.0 23 1132.0 24 1291.0 25 1443.0 26 1654.0 27 1930.0 28 2406.0 29 2992.0 30 3661.0 31 4703.0 32 6075.0 33 8286.0 34 11955.0 35 17905.0 36 28891.0 37 49173.0 38 46191.0 39 4221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.85765869829598 22.293125225973977 16.43400151197134 19.415214563758703 2 14.048430560641483 24.148615531956846 40.79180868144559 21.011145225956078 3 18.998145583437008 29.055186497969533 33.04664209760334 18.90002582099012 4 11.363668370724014 20.21490838931373 41.698236324118284 26.72318691584398 5 11.12535151096902 40.53537643949316 36.03603603603604 12.303236013501778 6 25.45805343135184 41.3771412208186 18.770919299036244 14.393886048793311 7 23.031062665183242 37.818337159247214 23.46388384243807 15.686716333131475 8 22.7414198600138 35.02142981208426 22.06777735528422 20.169372972617722 9 24.51307617558832 19.409354007351386 24.00655334450594 32.07101647255435 10-11 20.916247693889332 28.830021453283948 29.756221217626432 20.497509635200288 12-13 25.390454462236118 27.318198675247977 28.8682805920543 18.423066270461597 14-15 22.730388083803945 28.35988001182982 29.558118683134527 19.35161322123171 16-17 18.06723280803301 31.67903633914027 31.174860694485517 19.078870158341196 18-19 17.43120773468473 30.98756187959036 34.27208006966662 17.309150316058297 20-21 18.76535965354959 30.09682303097163 32.98109310267936 18.156724212799418 22-23 18.88123706113482 29.127175255021804 32.244004112270616 19.747583571572754 24-25 18.99915261995357 29.356437154211434 33.482074743145255 18.16233548268974 26-27 19.8408972475083 28.30705094198782 34.166717525715114 17.685334284788762 28-29 18.27556419393154 28.33340375487669 35.570625765834286 17.820406285357485 30-31 18.75942091595956 29.955108308250022 34.14280817254187 17.142662603248542 32-33 18.232795042718994 30.422964979814104 33.307435921509715 18.036804055957187 34-35 18.838654251061563 28.575921957265216 33.55616950258554 19.029254289087678 36-37 20.018874435703673 28.71379171919347 33.345540679808344 17.921793165294513 38-39 20.018027915099786 28.247724210457132 34.264775613489014 17.46947226095407 40-41 18.326138649652943 29.06769575290663 35.38023844566664 17.22592715177379 42-43 18.23557304088186 30.16656964188466 33.45078026703724 18.147077050196238 44-45 18.840457244794965 30.23378635307373 32.06370443396005 18.862051968171258 46-47 19.22579494269277 29.944112835270175 30.47716512885031 20.352927093186743 48-49 19.79034635570703 29.891605404238142 31.117088696729855 19.200959543324977 50-51 21.032284414566245 30.218108753925815 30.5993061456343 18.15030068587363 52-53 19.39577166919778 30.709971809563154 31.654269290262917 18.239987230976148 54-55 18.75498784140307 30.05309410472354 31.81256982977964 19.37934822409374 56-57 20.044080781905755 31.651315851242618 29.48154616041837 18.82305720643326 58-59 19.24246016191082 30.227186840579574 30.56213427472262 19.968218722786986 60-61 19.17792142558715 30.160406720464177 30.68875838532351 19.97291346862516 62-63 21.287572586740275 29.6620990418785 30.35897869224161 18.691349679139616 64-65 20.987744160224974 29.763310414764284 30.966828716364514 18.282116708646225 66-67 19.23368517905638 30.659634446611694 30.911958764338475 19.19472160999345 68-69 19.23307130601333 30.9879188896739 30.9111649927119 18.86784481160087 70-71 19.379424633353988 31.032993446378608 30.143606932942745 19.44397498732466 72-73 19.320603949332856 29.770702072319928 30.06788796138931 20.840806016957906 74-75 19.42692427766967 30.66450399866672 30.12133318623435 19.787238537429257 76-77 20.523166698355244 29.409113635243333 30.73346149602954 19.334258170371882 78-79 19.098066648513132 29.3519187973596 31.36297054432436 20.18704400980291 80-81 19.478596006966452 31.456288347158264 29.526431666361532 19.538683979513756 82-83 18.959349517161087 31.667848139297973 29.344259216660173 20.02854312688077 84-85 18.900103276687634 29.503333020373674 29.870199981222424 21.72636372171627 86-87 19.450429769834898 30.63477309748804 29.451086982034635 20.463710150642424 88-89 20.61023012139819 28.67973598475246 30.285234111671315 20.42479978217803 90-91 18.807286691062046 28.729899046326963 31.8139034872558 20.64891077535519 92-93 18.707589175689662 29.979509014390633 30.762766963742926 20.550134846176775 94-95 18.430793569572597 30.1342371671944 29.988475179385826 21.446494083847178 96-97 18.717960754858698 29.80706036991832 29.05149751197071 22.423481363252275 98-99 18.977706423310376 29.72617723135278 29.340299782650536 21.95581656268631 100-101 18.75950614965731 30.931367946671674 29.22401652427002 21.085109379400997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 799.0 1 509.5 2 207.5 3 180.0 4 150.5 5 127.5 6 163.5 7 250.5 8 326.0 9 388.5 10 381.5 11 327.0 12 337.5 13 374.0 14 399.0 15 420.5 16 451.5 17 519.5 18 556.5 19 606.5 20 697.0 21 771.5 22 931.5 23 1148.5 24 1316.5 25 1557.0 26 2029.0 27 2560.5 28 2914.0 29 3380.5 30 4005.5 31 4621.0 32 5655.0 33 6823.5 34 7912.5 35 8879.5 36 10048.0 37 11053.5 38 10871.5 39 10032.5 40 9136.0 41 8037.0 42 6858.5 43 6618.0 44 6812.5 45 6513.5 46 6083.0 47 7063.5 48 15520.0 49 15043.0 50 5966.0 51 3720.0 52 2535.5 53 2019.5 54 1613.0 55 1300.0 56 981.5 57 743.5 58 593.0 59 471.5 60 354.5 61 254.0 62 169.5 63 102.0 64 72.5 65 54.0 66 36.0 67 27.5 68 20.0 69 7.0 70 1.5 71 3.0 72 2.0 73 0.0 74 0.0 75 0.0 76 0.0 77 1.5 78 1.5 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02534961341839537 2 0.007041559282887603 3 0.007510996568413443 4 0.0 5 0.005633247426310082 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.003051342355917961 20-21 0.0016430304993404407 22-23 0.0 24-25 0.005398528783547163 26-27 0.006572121997361763 28-29 0.008449871139465123 30-31 0.03004398627365377 32-33 4.694372855258402E-4 34-35 0.004459654212495482 36-37 0.016195586350641486 38-39 0.008449871139465123 40-41 0.007745715211176363 42-43 0.007980433853939282 44-45 0.002816623713155041 46-47 0.0011735932138146005 48-49 0.0014083118565775205 50-51 0.0037554982842067214 52-53 0.0025819050703921207 54-55 0.0014083118565775205 56-57 0.0014083118565775205 58-59 0.0011735932138146005 60-61 0.0 62-63 0.0 64-65 0.009623464353279725 66-67 7.041559282887602E-4 68-69 0.0011735932138146005 70-71 0.0042249355697325615 72-73 0.010797057567094325 74-75 0.005867966069073002 76-77 0.004929091498021321 78-79 0.010797057567094325 80-81 0.0 82-83 0.004694372855258402 84-85 4.694372855258402E-4 86-87 0.0 88-89 0.0014083118565775205 90-91 0.0011735932138146005 92-93 2.347186427629201E-4 94-95 0.0011735932138146005 96-97 4.694372855258402E-4 98-99 0.0 100-101 4.694372855258402E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 213021.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.79924045047202 #Duplication Level Percentage of deduplicated Percentage of total 1 81.58196838933192 37.3639218668582 2 9.745597671224452 8.926819421559378 3 2.9027695209200304 3.988339177827538 4 1.428834997232529 2.617582304092085 5 0.7748918636354318 1.774472939287676 6 0.5083946618560505 1.3970453617249003 7 0.3853959533424899 1.2355589355040113 8 0.29417191119493247 1.077828007567329 9 0.2418974600766692 0.9970847944568845 >10 1.8388306922777309 17.031654156163007 >50 0.18859801972079293 5.907868238342698 >100 0.08917406367233144 6.95048844949559 >500 0.013324860088969066 5.185404255918431 >1k 0.00614993542567803 5.545932091202276 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2855 1.3402434501762737 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2776 1.3031579046197324 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1880 0.8825420967885794 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1734 0.8140042531018068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1537 0.7215251078532163 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1032 0.4844592786626671 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 992 0.46568178724163345 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 955 0.44831260767717734 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 940 0.4412710483942897 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 909 0.42671849254298877 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 904 0.4243713061153595 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 891 0.4182686214035236 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 889 0.417329746832472 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 889 0.417329746832472 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 860 0.40371606555222256 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 832 0.39057182155749903 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 773 0.36287502171147445 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 659 0.30935917116152867 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 553 0.2595988188957896 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 421 0.19763309720637873 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.19528591077874952 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 408 0.1915304124945428 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 380 0.17838616849981925 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 329 0.1544448669380014 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 328 0.1539754296524756 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 307 0.14411724665643294 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 304 0.1427089347998554 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 297 0.13942287380117455 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 271 0.1272175043775027 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 267 0.12533975523539934 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 252 0.11829819595251172 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 243 0.11407326038277915 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 241 0.11313438581172748 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 224 0.1051539519577882 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.694372855258402E-4 0.0 16-17 0.0 0.0 0.0 9.388745710516804E-4 0.0 18-19 0.0 0.0 0.0 0.0014083118565775205 0.0 20-21 0.0 0.0 0.0 0.002347186427629201 0.0 22-23 0.0 0.0 0.0 0.0032860609986808814 0.0 24-25 0.0 0.0 0.0 0.006337403354598843 0.0 26-27 0.0 0.0 0.0 0.009154027067753884 0.0 28-29 0.0 0.0 0.0 0.021828833776951568 0.0 30-31 0.0 0.0 0.0 0.0560977556203379 0.0 32-33 0.0 0.0 0.0 0.09858182996042644 0.0 34-35 0.0 0.0 0.0 0.1307382840189465 0.0 36-37 0.0 0.0 0.0 0.14646443308406215 0.0 38-39 0.0 0.0 0.0 0.16266001943470362 0.0 40-41 0.0 0.0 0.0 0.18894850742415067 0.0 42-43 0.0 0.0 0.0 0.21453283948530896 0.0 44-45 0.0 0.0 0.0 0.24316851390238522 0.0 46-47 0.0 0.0 0.0 0.2776721543885345 0.0 48-49 0.0 0.0 0.0 0.31475769994507585 0.0 50-51 0.0 0.0 0.0 0.349496059073988 0.0 52-53 0.0 0.0 0.0 0.4318823026837729 0.0 54-55 0.0 0.0 0.0 0.5440778139244488 0.0 56-57 0.0 0.0 0.0 0.6900728097229851 0.0 58-59 0.0 0.0 0.0 0.8867670323583121 0.0 60-61 0.0 0.0 0.0 1.1811042103830138 0.0 62-63 0.0 0.0 0.0 1.3439989484604804 0.0 64-65 0.0 0.0 0.0 1.480839917191263 0.0 66-67 0.0 0.0 0.0 1.806394674703433 0.0 68-69 0.0 0.0 0.0 2.2187953300378833 0.0 70-71 0.0 0.0 0.0 2.535430779125063 0.0 72-73 0.0 0.0 0.0 2.799489252233348 0.0 74-75 0.0 0.0 0.0 3.4010731336347124 0.0 76-77 0.0 0.0 0.0 3.7822562094816945 0.0 78-79 0.0 0.0 0.0 4.0089944183906745 0.0 80-81 0.0 0.0 0.0 4.234793752728605 0.0 82-83 0.0 0.0 0.0 4.6699621164110585 0.0 84-85 0.0 0.0 0.0 5.30276357729989 0.0 86-87 0.0 0.0 0.0 6.152445064101661 0.0 88-89 0.0 0.0 0.0 7.386360969106333 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACTC 50 2.8201293E-5 47.4973 8 GTATGGT 80 1.0739135E-5 35.622974 1 CCCTGTT 55 0.002575647 34.54349 2 CTATACA 75 3.0711194E-4 31.664865 4 CTATTCA 95 3.478997E-5 29.998295 9 CGTTGCT 40 4.490441E-4 29.685812 14-15 TTCGTTG 40 4.490441E-4 29.685812 12-13 GTGGTAC 80 4.482986E-4 29.685812 6 TCTATTC 100 4.9363538E-5 28.49838 8 GACCTAT 170 3.608875E-9 27.939589 6 CTCACAC 70 0.00837882 27.141315 3 ACTCCCG 45 8.941235E-4 26.38739 10-11 CCTATGC 180 6.6374923E-9 26.38739 8 TGGTACT 90 8.9265004E-4 26.38739 7 CAATACT 145 7.840936E-7 26.205408 4 ACCTATG 185 8.885763E-9 25.674217 7 ACTATAC 95 0.0012236815 24.998579 3 CTGGTCG 115 1.2774611E-4 24.7812 9 ATACACC 115 1.2774611E-4 24.7812 8 GAGTACT 1535 0.0 24.754295 12-13 >>END_MODULE