##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936945_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 219781 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.091918773688356 33.0 31.0 34.0 31.0 34.0 2 32.246613674521456 33.0 31.0 34.0 31.0 34.0 3 32.23274987373795 34.0 31.0 34.0 30.0 34.0 4 35.786132559229415 37.0 35.0 37.0 35.0 37.0 5 35.78991814579058 37.0 35.0 37.0 35.0 37.0 6 35.90973742043216 37.0 35.0 37.0 35.0 37.0 7 35.87816508251396 37.0 35.0 37.0 35.0 37.0 8 35.86618952502719 37.0 35.0 37.0 35.0 37.0 9 37.49642598768774 39.0 37.0 39.0 35.0 39.0 10-11 37.42311437294398 39.0 37.0 39.0 35.0 39.0 12-13 37.37674093756967 39.0 37.0 39.0 35.0 39.0 14-15 38.53801966503019 40.0 38.0 41.0 34.0 41.0 16-17 38.480569293979 40.0 38.0 41.0 34.0 41.0 18-19 38.517119314226434 40.0 38.0 41.0 34.0 41.0 20-21 38.44689258853131 40.0 38.0 41.0 34.0 41.0 22-23 38.32880230775181 40.0 38.0 41.0 34.0 41.0 24-25 38.45989871735955 40.0 38.0 41.0 34.0 41.0 26-27 38.35367479445448 40.0 38.0 41.0 34.0 41.0 28-29 38.247348496912835 40.0 38.0 41.0 34.0 41.0 30-31 38.09456458929571 40.0 38.0 41.0 34.0 41.0 32-33 37.89081403761017 40.0 37.0 41.0 34.0 41.0 34-35 37.7243073787088 40.0 37.0 41.0 33.0 41.0 36-37 37.503073514089024 40.0 37.0 41.0 33.0 41.0 38-39 37.2864760830099 40.0 36.0 41.0 33.0 41.0 40-41 37.10340520791151 39.5 36.0 41.0 32.5 41.0 42-43 36.91214436188751 39.0 36.0 41.0 32.0 41.0 44-45 36.60156246445325 39.0 36.0 41.0 31.0 41.0 46-47 36.171006592926595 39.0 36.0 41.0 30.0 41.0 48-49 35.84028419199112 39.0 35.5 41.0 29.0 41.0 50-51 35.09318366919797 38.5 35.0 40.0 27.5 40.5 52-53 35.19538313138988 38.5 35.5 40.0 27.5 40.5 54-55 35.642976417433715 39.0 36.0 41.0 28.0 41.0 56-57 35.618310954996105 39.0 36.0 41.0 27.5 41.0 58-59 35.43665284988238 39.0 35.5 41.0 26.5 41.0 60-61 35.1686633512451 39.0 35.0 41.0 26.0 41.0 62-63 34.81437885895505 39.0 35.0 40.5 25.5 41.0 64-65 34.39091641224674 38.0 34.5 40.0 22.5 41.0 66-67 34.054561131307985 38.0 34.0 40.0 22.0 41.0 68-69 33.688503555812375 37.0 34.0 40.0 21.5 41.0 70-71 33.2352136899914 37.0 34.0 39.0 19.0 41.0 72-73 32.76796674871804 36.0 33.0 39.0 16.0 40.5 74-75 32.27360645369709 36.0 33.0 38.5 11.5 40.0 76-77 31.772034434277757 35.0 32.0 37.5 7.5 39.5 78-79 31.308529854719016 35.0 31.5 37.0 4.5 39.0 80-81 30.865900601052864 35.0 31.0 36.5 2.0 39.0 82-83 30.43458715721559 35.0 31.0 36.0 2.0 37.5 84-85 30.012969728957465 35.0 31.0 36.0 2.0 37.0 86-87 29.60178313866986 34.0 30.5 35.0 2.0 37.0 88-89 29.15126876299589 34.0 29.5 35.0 2.0 36.0 90-91 28.722378185557442 34.0 29.0 35.0 2.0 36.0 92-93 28.41673984557355 34.0 29.0 35.0 2.0 36.0 94-95 28.097344629426566 34.0 29.0 35.0 2.0 35.0 96-97 27.60484755279119 34.0 28.5 35.0 2.0 35.0 98-99 27.00336926303912 34.0 26.5 35.0 2.0 35.0 100-101 24.614074920034035 31.5 20.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24824272193560404 1101 2 0.25659518501736756 1101 3 0.3610555007809211 1101 4 0.33443460911215084 1101 5 0.3687608413394514 1101 6 0.2991993507548827 1101 7 0.09374239585056898 1101 8 0.22881582203135054 1101 9 0.1956479509347986 1101 10-11 0.2668712863626368 1101 12-13 0.48421735534779486 1101 14-15 0.38704210481321155 1101 16-17 0.4764873514126151 1101 18-19 0.6355326588112007 1101 20-21 0.36381203030013154 1101 22-23 0.2327551921495612 1101 24-25 0.11452646378241838 1101 26-27 0.1366969972744485 1101 28-29 0.40561382670122015 1101 30-31 0.24478446898289263 1101 32-33 0.09173355759612178 1101 34-35 0.15581609336906155 1101 36-37 0.1399366740672221 1101 38-39 0.1539389185343012 1101 40-41 0.5980651199353773 1101 42-43 0.4112255103570206 1101 44-45 0.34884514317254656 1101 46-47 0.2267050253177203 1101 48-49 0.5943936206471037 1101 50-51 0.2308500934151212 1101 52-53 0.45989769698278593 1101 54-55 0.42662962961419737 1101 56-57 0.3233978103185535 1101 58-59 0.5787000029264604 1101 60-61 0.6244263158577112 1101 62-63 0.648606461941867 1101 64-65 0.4156742936148774 1101 66-67 0.6166758741032154 1101 68-69 0.49765129155758814 1101 70-71 0.6041213476299347 1101 72-73 0.7175951023940712 1101 74-75 0.6119159186557539 1101 76-77 0.49294864990680765 1101 78-79 0.5657354371940428 1101 80-81 0.7139855521738347 1101 82-83 0.5876622719597364 1101 84-85 0.14414575692445553 1101 86-87 0.309318474492251 1101 88-89 0.4512804910873385 1101 90-91 0.3307775237238424 1101 92-93 0.11516788527187316 1101 94-95 0.3888324469231783 1101 96-97 0.4779854642554042 1101 98-99 0.5006921419392363 1101 100-101 1.1873353215244613 1102 1 -0.06919140310986904 1102 2 0.21447159476737454 1102 3 0.16045522013023117 1102 4 0.14962092998944598 1102 5 0.06099357352593415 1102 6 -0.14104543901696331 1102 7 -0.04785627365725276 1102 8 -0.10864065112492938 1102 9 -0.22531509672384686 1102 10-11 -0.1302754315834136 1102 12-13 -0.13964574948043662 1102 14-15 -0.11519270626728684 1102 16-17 0.19347191562444976 1102 18-19 0.29162511762311283 1102 20-21 -0.01166221740008666 1102 22-23 0.07298568952496254 1102 24-25 0.05052901042760283 1102 26-27 -0.15534863444947433 1102 28-29 0.03084770227940936 1102 30-31 -0.07500140493218765 1102 32-33 -0.08593392330403304 1102 34-35 0.11171105724827868 1102 36-37 0.021023935644066682 1102 38-39 0.14608459703333665 1102 40-41 0.24972043930547017 1102 42-43 0.18461956472009433 1102 44-45 -0.02967598358801382 1102 46-47 0.09002423949577576 1102 48-49 0.3450692612063264 1102 50-51 0.20198594913987478 1102 52-53 0.4543626416322297 1102 54-55 0.5693457046622754 1102 56-57 0.7440815065921385 1102 58-59 0.8507102934518969 1102 60-61 0.6715912241265087 1102 62-63 0.7850728618171345 1102 64-65 0.9016547520110194 1102 66-67 0.8432883162839744 1102 68-69 0.8877102801756394 1102 70-71 0.6348629930745631 1102 72-73 0.46376214152925144 1102 74-75 0.5415453557952006 1102 76-77 0.5586352046596801 1102 78-79 0.30079338636508623 1102 80-81 0.44191029621000766 1102 82-83 0.3702489358545442 1102 84-85 0.6020611459374976 1102 86-87 0.3699832008578241 1102 88-89 0.3080446925321709 1102 90-91 0.18224704194014762 1102 92-93 0.23541891172580165 1102 94-95 -0.052815596317820734 1102 96-97 0.193443340665155 1102 98-99 0.1403938206461639 1102 100-101 0.045190942334190964 1103 1 0.2620165413225166 1103 2 0.36745889669921894 1103 3 0.49755094402864586 1103 4 0.6059238386067705 1103 5 0.492103840510957 1103 6 0.38582942531991904 1103 7 0.2140820810204147 1103 8 0.39979759502223544 1103 9 0.4284047860632185 1103 10-11 0.3101345423692692 1103 12-13 0.5675593188937711 1103 14-15 0.7199698181520731 1103 16-17 0.6785896712137713 1103 18-19 0.4047952934425183 1103 20-21 0.4062208952545632 1103 22-23 0.779539574916754 1103 24-25 0.4773319733101715 1103 26-27 0.660386930994413 1103 28-29 0.7635777869331974 1103 30-31 1.0191216686514935 1103 32-33 0.7589352974469961 1103 34-35 0.8516787694253978 1103 36-37 0.8302069723273746 1103 38-39 1.0125242540769648 1103 40-41 0.8812051365053861 1103 42-43 0.9511229834307571 1103 44-45 0.9206916634708122 1103 46-47 1.2088922664113326 1103 48-49 1.5435444077225 1103 50-51 1.5193494720398135 1103 52-53 1.52055946168867 1103 54-55 1.791480499539631 1103 56-57 1.4050051011221996 1103 58-59 1.4899210054119578 1103 60-61 1.7206877077384064 1103 62-63 1.6290331396551991 1103 64-65 1.7538836970945866 1103 66-67 1.4342504391404987 1103 68-69 1.9064385740762262 1103 70-71 1.939379218383877 1103 72-73 1.3072543815987103 1103 74-75 1.7468579236267558 1103 76-77 1.7732406963028353 1103 78-79 1.664974252438455 1103 80-81 1.9467941685781405 1103 82-83 1.7188760250666135 1103 84-85 1.7950622855077185 1103 86-87 1.754533884599958 1103 88-89 1.6357253958097928 1103 90-91 1.6699966620834097 1103 92-93 2.088034496705177 1103 94-95 2.0684875836257426 1103 96-97 2.3818794162023806 1103 98-99 2.1418996812930082 1103 100-101 2.388479687721645 1104 1 0.441011273543765 1104 2 0.46507742172116906 1104 3 0.29079062796191835 1104 4 0.4841613276322292 1104 5 0.37149365613869634 1104 6 0.5084698994200068 1104 7 0.3710179897122501 1104 8 0.4141742412030993 1104 9 0.6238064541931578 1104 10-11 0.526909125336779 1104 12-13 0.3861776244249455 1104 14-15 0.6068497566946505 1104 16-17 0.41602382397935855 1104 18-19 0.4190621942326942 1104 20-21 0.7588877082484231 1104 22-23 0.509373859376808 1104 24-25 0.5513826756806779 1104 26-27 0.6556953155422605 1104 28-29 0.5181322206469829 1104 30-31 0.5656537143055758 1104 32-33 0.7408821806779002 1104 34-35 0.7168839933059914 1104 36-37 0.3837352866381778 1104 38-39 0.4072235517064655 1104 40-41 0.2598267343982812 1104 42-43 0.298549454018989 1104 44-45 0.15129416566571763 1104 46-47 -0.35585751409787747 1104 48-49 -0.29469088639515206 1104 50-51 -0.17103134446589507 1104 52-53 0.004673816385768248 1104 54-55 -0.056631879740436375 1104 56-57 0.03582160682896074 1104 58-59 0.1320687227078352 1104 60-61 0.29076013969626757 1104 62-63 0.4038355979519608 1104 64-65 -0.010355767347910216 1104 66-67 -0.2806859085329023 1104 68-69 -0.06670124155151314 1104 70-71 -0.09642521532990855 1104 72-73 0.026114126989405406 1104 74-75 -0.007092910438210254 1104 76-77 -0.13770091914931726 1104 78-79 -0.14760805660457166 1104 80-81 0.16024895347717916 1104 82-83 0.2677127239252606 1104 84-85 0.19911737769384885 1104 86-87 -0.11432037352120261 1104 88-89 -0.49937118013401616 1104 90-91 -0.1871697119288811 1104 92-93 0.06103713059455629 1104 94-95 0.15433591549580328 1104 96-97 0.5114887226115101 1104 98-99 0.6693909016617496 1104 100-101 0.2816699862291081 1105 1 0.10235703436620724 1105 2 0.25025423475145203 1105 3 0.004019091453592694 1105 4 0.06899132701291677 1105 5 0.11966669137743935 1105 6 0.29339898841486445 1105 7 0.20767495385419465 1105 8 0.34423864945230775 1105 9 0.3209328553822317 1105 10-11 0.5201586453714597 1105 12-13 0.37573450805657416 1105 14-15 0.36851601048793015 1105 16-17 0.2886549628906039 1105 18-19 -0.04641900970612767 1105 20-21 0.07163156159579387 1105 22-23 0.21414874015961516 1105 24-25 0.19280213440365657 1105 26-27 -0.2088847922176953 1105 28-29 -0.025490433716058192 1105 30-31 -0.2660565105039865 1105 32-33 0.06496715002204922 1105 34-35 -0.08802558742107891 1105 36-37 -0.024343613467102898 1105 38-39 -0.20515026386299695 1105 40-41 -0.41590250323100264 1105 42-43 0.17769883161235356 1105 44-45 -0.2910867719697521 1105 46-47 0.48644291322607813 1105 48-49 0.6705580762604484 1105 50-51 0.7385571239802502 1105 52-53 0.7379059957379681 1105 54-55 0.40494767064043913 1105 56-57 0.37007100300162676 1105 58-59 0.3737002951361461 1105 60-61 0.4594447250681597 1105 62-63 0.7094883507852998 1105 64-65 0.0917452421275371 1105 66-67 0.08706042205482589 1105 68-69 0.411266802047912 1105 70-71 0.03589038509122844 1105 72-73 0.15091285121062015 1105 74-75 0.4010866275564595 1105 76-77 0.41058204363014994 1105 78-79 0.39118397906769076 1105 80-81 0.5877509662349674 1105 82-83 0.6968172015372005 1105 84-85 0.9697404036429411 1105 86-87 0.8951550708337876 1105 88-89 0.7898930794807733 1105 90-91 0.2173636379193873 1105 92-93 0.7381443331708866 1105 94-95 0.7881223773772703 1105 96-97 0.7426616959192458 1105 98-99 0.7097389539315238 1105 100-101 0.10022761303284966 1106 1 0.14379344563262464 1106 2 0.27402232852356434 1106 3 0.4080958071351759 1106 4 0.25479548403913554 1106 5 0.30916130865950464 1106 6 0.39472411222693893 1106 7 0.5173485488978855 1106 8 0.5460093055128823 1106 9 0.3099953255048007 1106 10-11 0.3040946264028719 1106 12-13 0.2984738621304217 1106 14-15 0.4407190049235581 1106 16-17 0.6938885326938475 1106 18-19 0.4564014197640205 1106 20-21 0.3668301389520465 1106 22-23 0.44688675572455594 1106 24-25 0.39899943061567456 1106 26-27 0.26159152573553257 1106 28-29 0.43711736405436596 1106 30-31 -0.010391749619039103 1106 32-33 0.06411624079208167 1106 34-35 -0.024905331957093324 1106 36-37 0.05429378447158939 1106 38-39 0.03886946958251514 1106 40-41 0.26240600394913827 1106 42-43 0.3532729563231314 1106 44-45 0.4611074265502353 1106 46-47 0.7516206480865932 1106 48-49 0.3072676389504707 1106 50-51 0.3658780028069515 1106 52-53 0.7294829504390066 1106 54-55 0.5683509251292236 1106 56-57 0.49456189158031805 1106 58-59 0.4694801677865854 1106 60-61 0.645650434764697 1106 62-63 0.2915771895332213 1106 64-65 0.5996328838660716 1106 66-67 0.6379811249632112 1106 68-69 0.20991682021339386 1106 70-71 0.3849352633825234 1106 72-73 0.0848956800535774 1106 74-75 0.17458223915942028 1106 76-77 0.4036791620116986 1106 78-79 0.8142101564774862 1106 80-81 0.8256231195133559 1106 82-83 0.9087987683574781 1106 84-85 1.0545923071938148 1106 86-87 0.9032530496853468 1106 88-89 0.9717581960266521 1106 90-91 0.8870100803539422 1106 92-93 0.7874110215086496 1106 94-95 0.5361193944145732 1106 96-97 0.8973646425509862 1106 98-99 0.6813243221170779 1106 100-101 0.7307895955557449 1107 1 0.1481248473058514 1107 2 0.020150767438934736 1107 3 0.039333322215973965 1107 4 0.027960571754967134 1107 5 0.1384368372289515 1107 6 -0.03709092307695272 1107 7 -0.028091612491323303 1107 8 0.10501723652690487 1107 9 0.23026984539166762 1107 10-11 0.2406366251766343 1107 12-13 0.4325792002368729 1107 14-15 0.2636062104772421 1107 16-17 0.42661731574797557 1107 18-19 0.26275414221808546 1107 20-21 0.4529736092156895 1107 22-23 0.3350416577241262 1107 24-25 0.20316667414329714 1107 26-27 0.06807280577350383 1107 28-29 0.017980084332840818 1107 30-31 0.12733297892668105 1107 32-33 0.11119798363735356 1107 34-35 0.21340087242969474 1107 36-37 0.3849043341047036 1107 38-39 0.3605897678361103 1107 40-41 0.3998241721024627 1107 42-43 0.7915752050919522 1107 44-45 0.6076630722059733 1107 46-47 0.40066833306116933 1107 48-49 0.5663606157507743 1107 50-51 0.2759371392955501 1107 52-53 0.7174084265965206 1107 54-55 0.16812149928716735 1107 56-57 0.35220909001389344 1107 58-59 0.08726858178156505 1107 60-61 0.21253004553189214 1107 62-63 0.3508521247599745 1107 64-65 -0.030269610151059112 1107 66-67 0.21803294355857616 1107 68-69 0.18137703658569393 1107 70-71 -0.12685247815740297 1107 72-73 0.2048102387054982 1107 74-75 -0.0018482111876849672 1107 76-77 0.06223021915059945 1107 78-79 0.2120662115396854 1107 80-81 0.19521628420551096 1107 82-83 0.19252535604363885 1107 84-85 0.03399342663369964 1107 86-87 0.0021039426661033644 1107 88-89 0.156080104216759 1107 90-91 0.19992912863225953 1107 92-93 0.26917562268346984 1107 94-95 0.6605334685032993 1107 96-97 0.6368970885073573 1107 98-99 0.8225201054283282 1107 100-101 1.0484850525285125 1108 1 -0.22249098261729117 1108 2 0.16655260394956173 1108 3 -0.10764741438448766 1108 4 0.22215160878460694 1108 5 0.12298619345212813 1108 6 0.26659207921868955 1108 7 0.21332968703956823 1108 8 0.1400506729976172 1108 9 0.2330046492643092 1108 10-11 0.17286539052247463 1108 12-13 0.2968770343520504 1108 14-15 0.3745953311479653 1108 16-17 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54-55 -0.20630910710056583 2316 56-57 -0.03378053583196561 2316 58-59 -0.44348525586556065 2316 60-61 -0.15514682853008566 2316 62-63 0.09686966076672121 2316 64-65 0.1557805754778414 2316 66-67 -0.05611856122038006 2316 68-69 0.33763444199394854 2316 70-71 -0.5551286307680598 2316 72-73 -0.7750602160194795 2316 74-75 -0.8772398750018979 2316 76-77 -0.39075695797360055 2316 78-79 -0.743238035544227 2316 80-81 -1.6223144564669667 2316 82-83 -0.5639189947818153 2316 84-85 -0.1807699952573465 2316 86-87 -0.45554370471797867 2316 88-89 -0.20390831043344448 2316 90-91 0.01272040802350105 2316 92-93 -0.9881112990364258 2316 94-95 -0.19638746148432418 2316 96-97 -0.3924349107553944 2316 98-99 -0.17393846378097422 2316 100-101 -0.5618654043028961 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 438.0 3 110.0 4 103.0 5 91.0 6 110.0 7 123.0 8 166.0 9 176.0 10 224.0 11 376.0 12 637.0 13 857.0 14 1028.0 15 1417.0 16 2087.0 17 3104.0 18 2701.0 19 1212.0 20 1130.0 21 1125.0 22 1321.0 23 1329.0 24 1506.0 25 1682.0 26 1892.0 27 2210.0 28 2602.0 29 3230.0 30 3942.0 31 4852.0 32 6414.0 33 8656.0 34 12118.0 35 18122.0 36 27712.0 37 44879.0 38 50106.0 39 9989.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.9566938989145 21.97619643629247 16.018933618551305 21.048176046241725 2 13.550630002593728 22.977689398937937 41.28485036016399 22.18683023830434 3 17.61592718998862 28.97519908987486 33.1745164960182 20.234357224118316 4 10.719633015832564 19.006357600222085 42.14947869498537 28.124530688959986 5 10.69251396546308 39.77118038324782 36.965340156340346 12.57096549494876 6 24.35261933698388 41.1050875539958 19.903683776906867 14.63860933211345 7 22.56164988236579 36.86979235400389 24.00192947408179 16.56662828954853 8 21.49818598955749 34.5372111125779 23.2821525953778 20.68245030248681 9 23.61776110979628 19.11194451018588 24.166196362577143 33.1040980174407 10-11 20.468389830315637 27.882665889961157 30.902260258542814 20.746684021180396 12-13 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32.84656089470882 19.119032127435947 38-39 19.409776095294863 28.533176207224464 33.46308370605284 18.59396399142783 40-41 17.708764633885547 29.37287572629117 34.47750260486575 18.440857034957524 42-43 17.386398278286112 30.252615103216385 32.89365322753105 19.467333390966463 44-45 18.18359681591406 30.49952111311058 31.587424726033476 19.729457344941885 46-47 18.402637170638044 29.819456640928927 30.42050950719125 21.35739668124178 48-49 18.758076258076258 29.704477204477204 31.11111111111111 20.426335426335427 50-51 19.965329123081613 30.08449320004914 30.982887510749336 18.96729016611991 52-53 18.327729218897165 30.516564366932236 32.07085299323411 19.084853420936486 54-55 17.97047047047047 29.7959322959323 31.964009464009465 20.26958776958777 56-57 19.08126726150122 31.50499815725654 29.59582493482148 19.817909646420755 58-59 18.356454834585335 29.81217666677283 30.947625135930767 20.883743362711062 60-61 18.705536811595522 29.406277798379705 31.29048183478975 20.597703555235025 62-63 20.43575591844535 29.298295105537836 30.74998066239268 19.51596831362414 64-65 19.917553354855002 29.513350941971122 31.695320515883036 18.87377518729084 66-67 18.437337895761853 30.010784197737593 31.276448586223527 20.275429320277023 68-69 18.038747255497537 30.727733979500133 31.63396016063161 19.599558604370728 70-71 18.609345062826822 30.331860320118693 30.37213684004533 20.686657777009163 72-73 18.391557794432842 29.403239091161442 30.254649538345323 21.950553576060393 74-75 18.704991774800174 30.010261590933396 30.757924291756826 20.526822342509607 76-77 19.615374987768554 28.859846941910288 31.274278003190414 20.25050006713074 78-79 18.29798853190134 28.804496222808773 31.89314644579958 21.004368799490305 80-81 18.27262940599445 30.873767846453926 30.571974154078653 20.281628593472973 82-83 18.251228332085468 30.486986165760545 30.2491710607464 21.012614441407592 84-85 18.31577510296025 28.845024915242668 30.5290222758197 22.31017770597738 86-87 19.013317012238545 29.37933576481242 30.55358999620034 21.053757226748697 88-89 20.174326934671765 27.659100061658314 31.20957228242693 20.957000721242995 90-91 18.17416452588394 27.75296315426605 32.84055510932213 21.232317210527874 92-93 18.33707282218389 28.825087494936895 31.88502095782494 20.95281872505427 94-95 17.793168409868105 28.964977463897867 31.41016160922103 21.831692517012993 96-97 17.660657442359458 28.24153395632675 30.40676600886025 23.69104259245354 98-99 18.03845357376109 28.1620429684211 31.423474020284953 22.376029437532853 100-101 18.50842874992319 28.408540040829422 31.359455971815407 21.72357523743198 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 593.0 1 378.0 2 163.0 3 147.5 4 126.0 5 114.5 6 168.5 7 314.0 8 447.0 9 489.0 10 410.5 11 328.5 12 340.5 13 356.5 14 372.0 15 412.0 16 413.5 17 403.0 18 451.0 19 499.0 20 594.0 21 722.0 22 825.5 23 961.5 24 1155.0 25 1423.0 26 1986.5 27 2684.5 28 3064.0 29 3431.0 30 4102.5 31 4730.0 32 5622.5 33 6963.0 34 8474.0 35 9512.5 36 10733.5 37 12072.5 38 11760.5 39 10557.0 40 9473.0 41 8358.0 42 7169.5 43 6912.5 44 7091.5 45 6792.5 46 6410.0 47 7329.0 48 15520.0 49 15095.0 50 5991.5 51 3611.0 52 2629.5 53 2097.0 54 1672.0 55 1368.5 56 1056.5 57 817.5 58 635.5 59 483.5 60 376.0 61 281.5 62 184.0 63 120.5 64 86.0 65 65.5 66 46.5 67 31.5 68 22.0 69 13.5 70 10.0 71 6.0 72 4.5 73 4.0 74 3.5 75 2.5 76 0.5 77 0.5 78 0.5 79 0.0 80 1.0 81 1.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03002989339387845 2 0.009099967695114682 3 0.014104949927427756 4 0.020019928929252303 5 0.0600597867877569 6 0.040039857858504606 7 0.01455994831218349 8 0.04640983524508488 9 0.029574895009122717 10-11 0.022522420045408838 12-13 0.035944872395702994 14-15 0.019564930544496567 16-17 0.016607441043584293 18-19 0.016834940235962163 20-21 0.01569744427407283 22-23 0.006597476578958145 24-25 0.0011374959618893352 26-27 2.2749919237786704E-4 28-29 0.0011374959618893352 30-31 0.0 32-33 2.2749919237786704E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.2749919237786704E-4 46-47 0.0 48-49 4.549983847557341E-4 50-51 0.0 52-53 9.099967695114682E-4 54-55 4.549983847557341E-4 56-57 0.0 58-59 0.0 60-61 0.002957489500912272 62-63 9.099967695114682E-4 64-65 0.0011374959618893352 66-67 0.006824975771336012 68-69 0.010692462041759752 70-71 0.022749919237786707 72-73 0.012739954773160556 74-75 0.013422452350294154 76-77 0.02752740227772191 78-79 0.0186549337749851 80-81 0.042997347359416876 82-83 0.03298738289479072 84-85 0.01637994185120643 86-87 0.011147460426515486 88-89 0.009782465272248284 90-91 0.018427434582607233 92-93 0.024569912776809644 94-95 0.010237463657004018 96-97 0.01615244265882856 98-99 0.02798240066247765 100-101 0.039357360281371004 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 219781.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.301185986844665 #Duplication Level Percentage of deduplicated Percentage of total 1 81.58182093226414 37.773350641302194 2 9.923978217398314 9.189839223463093 3 2.7432089457701347 3.8104148279663734 4 1.3170015247667846 2.4391493017273964 5 0.7579984449519149 1.7548113488728825 6 0.5449988071004146 1.5140454678098865 7 0.4099991001814791 1.3288411214359137 8 0.2789993876143895 1.0334402028919691 9 0.24599946004445353 1.0251060076963445 >10 1.8839958647297927 17.308734365715495 >50 0.21499952808752895 6.774774663326882 >100 0.07899982659960367 6.287687255876098 >500 0.009999978050582744 3.379979158166091 >1k 0.007999982440466194 6.3798264137493925 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2921 1.3290502818714995 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2292 1.0428562978601426 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2039 0.9277417065169418 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1626 0.7398273736128237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1299 0.5910429017976986 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1279 0.5819429341025839 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1198 0.5450880649373695 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1125 0.5118731828502009 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 974 0.443168426752085 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 915 0.4163235220514967 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 797 0.36263371265032013 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 780 0.3548987401094726 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 699 0.3180438709442581 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 698 0.3175888725595024 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 688 0.3130388887119451 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 650 0.2957489500912272 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 596 0.2711790373144175 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 503 0.22886418753213425 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 495 0.2252242004540884 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 468 0.21293924406568357 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 427 0.19428431029069848 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 426 0.1938293119059427 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 375 0.1706243942834003 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 360 0.16379941851206428 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 335 0.15242445889317094 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 333 0.15151446212365946 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 296 0.1346795218876973 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 234 0.10646962203284178 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 233 0.10601462364808606 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 231 0.10510462687857458 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 225 0.10237463657004017 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 221 0.10055464303101724 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.5499838475573413E-4 0.0 24-25 0.0 0.0 0.0 0.0020474927314008034 0.0 26-27 0.0 0.0 0.0 0.004777483039935209 0.0 28-29 0.0 0.0 0.0 0.018882432967362965 0.0 30-31 0.0 0.0 0.0 0.04640983524508488 0.0 32-33 0.0 0.0 0.0 0.08258220683316574 0.0 34-35 0.0 0.0 0.0 0.11170210345753273 0.0 36-37 0.0 0.0 0.0 0.12626205176971622 0.0 38-39 0.0 0.0 0.0 0.13900200654287675 0.0 40-41 0.0 0.0 0.0 0.15993193224164054 0.0 42-43 0.0 0.0 0.0 0.18108935713278218 0.0 44-45 0.0 0.0 0.0 0.20088178686965663 0.0 46-47 0.0 0.0 0.0 0.2313666786482908 0.0 48-49 0.0 0.0 0.0 0.2770940163162421 0.0 50-51 0.0 0.0 0.0 0.31849886932901383 0.0 52-53 0.0 0.0 0.0 0.40108107616217964 0.0 54-55 0.0 0.0 0.0 0.5152856707358688 0.0 56-57 0.0 0.0 0.0 0.6570176675872801 0.0 58-59 0.0 0.0 0.0 0.8308270505639705 0.0 60-61 0.0 0.0 0.0 1.105873574148812 0.0 62-63 0.0 0.0 0.0 1.2760429700474565 0.0 64-65 0.0 0.0 0.0 1.4093574967808864 0.0 66-67 0.0 0.0 0.0 1.7151164113367399 0.0 68-69 0.0 0.0 0.0 2.1221124665007443 0.0 70-71 0.0 0.0 0.0 2.4415213325992693 0.0 72-73 0.0 0.0 0.0 2.7165678561841107 0.0 74-75 0.0 0.0 0.0 3.3335456659128857 0.0 76-77 0.0 0.0 0.0 3.7086918341439885 0.0 78-79 0.0 0.0 0.0 3.9418785063313027 0.0 80-81 0.0 0.0 0.0 4.180980157520441 0.0 82-83 0.0 0.0 0.0 4.627788571350571 0.0 84-85 0.0 0.0 0.0 5.2379414053080104 0.0 86-87 0.0 0.0 0.0 6.054891005136931 0.0 88-89 0.0 0.0 0.0 7.209449406454607 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 55 1.4915713E-10 69.150566 2 CATTCCG 45 1.50827655E-5 52.799286 9 TTGCGTT 75 2.3519533E-9 50.71041 4 TTTGCGT 75 2.3519533E-9 50.71041 3 GTTCGCA 40 5.367809E-4 47.51936 9 ACAGTAC 45 9.567311E-4 42.25868 8 CGCAGAA 65 1.3215157E-4 36.553352 9 GCTTTGC 105 4.602407E-8 36.213474 1 CCTTTAG 70 2.040316E-4 33.95013 1 ACAGTCT 60 0.003927423 31.694006 8 AAGACGC 30 0.003958056 31.65074 38-39 GGCATTC 80 4.4593358E-4 29.713133 7 GCATTCC 85 6.358376E-4 27.965302 8 GAGTACT 1045 0.0 25.692284 12-13 ATCCAGG 95 0.001218873 25.015884 1 ATGGGTA 120 1.6947335E-4 23.770504 5 CTTTAGG 120 1.6947335E-4 23.770504 2 CGGTGTT 50 0.0016481108 23.75968 14-15 GTATAGG 190 3.242003E-7 22.514297 1 GTACTTT 1195 0.0 22.467312 14-15 >>END_MODULE