##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936945_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 219781 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35794722928733 33.0 31.0 34.0 31.0 34.0 2 32.49731323453802 34.0 31.0 34.0 31.0 34.0 3 32.58023669015975 34.0 31.0 34.0 31.0 34.0 4 36.18067530860265 37.0 35.0 37.0 35.0 37.0 5 36.10262488568166 37.0 35.0 37.0 35.0 37.0 6 36.179797161720074 37.0 36.0 37.0 35.0 37.0 7 36.140867499920375 37.0 35.0 37.0 35.0 37.0 8 36.13254557946319 37.0 35.0 37.0 35.0 37.0 9 37.82484837178828 39.0 38.0 39.0 35.0 39.0 10-11 37.76889494542294 39.0 37.0 39.0 35.0 39.0 12-13 37.70083401203925 39.0 37.0 39.0 35.0 39.0 14-15 38.871098957598704 40.0 38.0 41.0 35.5 41.0 16-17 38.79433163012271 40.0 38.0 41.0 35.5 41.0 18-19 39.01316537826291 40.0 38.5 41.0 36.0 41.0 20-21 39.08087368789841 40.0 38.5 41.0 35.5 41.0 22-23 39.02414676427899 40.0 38.0 41.0 35.0 41.0 24-25 38.93023737265733 40.0 38.0 41.0 35.0 41.0 26-27 38.700383563638354 40.0 38.0 41.0 34.5 41.0 28-29 38.582459357269286 40.0 38.0 41.0 34.5 41.0 30-31 38.55184934093484 40.0 38.0 41.0 35.0 41.0 32-33 38.34392872905301 40.0 38.0 41.0 34.0 41.0 34-35 38.19034174928679 40.0 37.5 41.0 34.0 41.0 36-37 38.01279682957126 40.0 37.0 41.0 34.0 41.0 38-39 37.784724339228596 40.0 37.0 41.0 33.5 41.0 40-41 37.56470759528804 40.0 37.0 41.0 33.0 41.0 42-43 37.31386243578835 40.0 37.0 41.0 33.0 41.0 44-45 36.98031676987547 40.0 37.0 41.0 32.5 41.0 46-47 36.45400648827697 40.0 37.0 41.0 31.0 41.0 48-49 35.976865607127095 40.0 36.0 41.0 29.5 41.0 50-51 35.67489910410818 39.5 36.0 41.0 28.0 41.0 52-53 35.374461395662046 39.0 35.0 40.5 27.0 41.0 54-55 35.13234765516582 39.0 35.0 40.0 26.0 41.0 56-57 34.93906206632966 39.0 35.0 40.0 25.0 41.0 58-59 34.671286416933214 39.0 35.0 40.0 23.5 41.0 60-61 34.36976353733944 38.0 34.5 40.0 22.5 41.0 62-63 33.98301718528899 38.0 34.0 40.0 22.0 41.0 64-65 33.53033019232782 37.5 33.5 40.0 20.0 41.0 66-67 33.13523234492517 37.0 33.0 39.0 18.0 41.0 68-69 32.87465249498364 36.5 33.0 39.0 17.5 40.0 70-71 32.393971726400366 36.0 32.5 39.0 10.5 40.0 72-73 31.863552809387528 35.5 32.0 38.0 7.5 40.0 74-75 31.358320327962836 35.0 31.0 37.0 6.5 39.5 76-77 29.8567574085112 33.5 29.5 36.0 2.0 38.5 78-79 30.512109782010274 34.0 31.0 36.0 2.0 39.0 80-81 30.253675249452865 34.0 31.0 36.0 2.0 38.0 82-83 29.961366087150388 34.0 30.5 35.5 2.0 37.0 84-85 29.639306855460664 34.0 30.0 35.0 2.0 37.0 86-87 29.30221675213053 34.0 30.0 35.0 2.0 36.0 88-89 28.832119245976678 34.0 29.0 35.0 2.0 36.0 90-91 28.373130525386635 34.0 29.0 35.0 2.0 35.5 92-93 28.0141117749032 34.0 29.0 35.0 2.0 35.0 94-95 27.54432366765098 33.5 27.5 35.0 2.0 35.0 96-97 26.922768574171563 33.0 26.0 35.0 2.0 35.0 98-99 26.231717027404553 33.0 25.0 34.0 2.0 35.0 100-101 22.97780745378354 29.0 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05183572082784593 1101 2 -0.26790134975852453 1101 3 0.0020258830395007976 1101 4 0.1293281625010252 1101 5 0.03861264016877186 1101 6 -0.15286740035069357 1101 7 0.16362186947269208 1101 8 0.04849602214349602 1101 9 0.021610814705738335 1101 10-11 0.07959108176456198 1101 12-13 -0.008979403841877343 1101 14-15 0.04658401074868834 1101 16-17 -0.03287220776354616 1101 18-19 -0.1280454701108127 1101 20-21 0.05604289333847845 1101 22-23 -0.035777299661688744 1101 24-25 0.1009496990120482 1101 26-27 -0.11734850017420939 1101 28-29 0.06854474397479748 1101 30-31 -2.542540258971826E-4 1101 32-33 0.22978631072460587 1101 34-35 0.33050445617369206 1101 36-37 0.16263652511597826 1101 38-39 0.1575569776871788 1101 40-41 0.43193714950887596 1101 42-43 0.4002075542791772 1101 44-45 0.5965315299338343 1101 46-47 0.5908595538359194 1101 48-49 0.9723282974870386 1101 50-51 1.1587442420891207 1101 52-53 0.8078097757786153 1101 54-55 0.5318527586635966 1101 56-57 0.93626715452627 1101 58-59 0.8354232943746212 1101 60-61 0.8505263976811435 1101 62-63 1.120478020138016 1101 64-65 0.9310143802148261 1101 66-67 0.49606014226871764 1101 68-69 0.5244210631009949 1101 70-71 0.5549650458764361 1101 72-73 0.7166622653781722 1101 74-75 0.5083651353043273 1101 76-77 0.6071020102555984 1101 78-79 0.43808748067802483 1101 80-81 0.9310495275177431 1101 82-83 0.9568086881483566 1101 84-85 0.9592186580178534 1101 86-87 0.3958142784757541 1101 88-89 0.3537790469397777 1101 90-91 0.69629555218566 1101 92-93 0.8143389486709864 1101 94-95 0.27102158929945475 1101 96-97 0.16322926266643023 1101 98-99 0.5728992091352119 1101 100-101 1.3192237508246372 1102 1 0.21852404264063807 1102 2 0.3727228381734946 1102 3 0.1659206910016664 1102 4 0.17002251310650252 1102 5 0.16680244319356063 1102 6 0.2716557533315509 1102 7 0.1996127223389621 1102 8 0.08209614687714151 1102 9 0.39106926281134236 1102 10-11 0.29682901118610516 1102 12-13 0.36704705008462923 1102 14-15 0.2760874149376562 1102 16-17 0.2123398394273508 1102 18-19 0.2602854378461501 1102 20-21 0.1615012894717367 1102 22-23 0.18234415972191442 1102 24-25 0.08996404338084574 1102 26-27 0.07824944172069337 1102 28-29 0.10751101391918638 1102 30-31 0.33579246911738636 1102 32-33 0.46511711473687 1102 34-35 0.615962592445193 1102 36-37 0.5820728849725327 1102 38-39 0.27734674952856153 1102 40-41 0.3838562285588196 1102 42-43 0.27269988835744385 1102 44-45 -0.07975868367253014 1102 46-47 0.020976231763263797 1102 48-49 0.298506770539376 1102 50-51 0.4380956271522649 1102 52-53 0.20157179438471218 1102 54-55 0.2778443911151385 1102 56-57 -0.09442901511147994 1102 58-59 0.3577649086358363 1102 60-61 0.3393978180854944 1102 62-63 0.4223009503391424 1102 64-65 0.30314940438196913 1102 66-67 0.3202766590817774 1102 68-69 0.23149190141500497 1102 70-71 0.4796765993300056 1102 72-73 0.312687679857671 1102 74-75 0.5756953156483817 1102 76-77 0.11634659213803644 1102 78-79 0.22115488881689416 1102 80-81 0.47193227789454184 1102 82-83 0.245495606707685 1102 84-85 0.45567154508193397 1102 86-87 0.23891624822784863 1102 88-89 0.5496173609567236 1102 90-91 0.5003597900111352 1102 92-93 0.6615207479537659 1102 94-95 1.229989418414366 1102 96-97 1.365843471907116 1102 98-99 1.0312173838662524 1102 100-101 1.995299578484321 1103 1 0.31203663217829813 1103 2 0.29242383665406635 1103 3 0.2661621713576494 1103 4 0.17608665463027506 1103 5 0.13642747032618985 1103 6 0.30317029641815907 1103 7 0.17268359275355039 1103 8 0.2100536029222866 1103 9 0.1292123060064796 1103 10-11 0.46942641730722556 1103 12-13 0.42013202946383643 1103 14-15 0.5164214175423965 1103 16-17 0.5359709985330596 1103 18-19 0.5442826160532235 1103 20-21 0.4915493142166838 1103 22-23 0.7404758072148638 1103 24-25 0.507277370925884 1103 26-27 0.6084955346228043 1103 28-29 0.40457933386709755 1103 30-31 0.4907875852450232 1103 32-33 0.7399095501612294 1103 34-35 0.8522267014098119 1103 36-37 0.6886566162510235 1103 38-39 0.6102030008851926 1103 40-41 0.3346919133862585 1103 42-43 0.3020302551905232 1103 44-45 0.43934946696779775 1103 46-47 0.6887779465451231 1103 48-49 0.7923883637670741 1103 50-51 0.9306477217908551 1103 52-53 1.1558058404016478 1103 54-55 1.1407228912236604 1103 56-57 1.1970635505494656 1103 58-59 1.1725711816985651 1103 60-61 1.2840133487996255 1103 62-63 1.3072789729134868 1103 64-65 1.475183808549538 1103 66-67 1.1555205813739349 1103 68-69 1.2094873431341355 1103 70-71 1.3740153772766064 1103 72-73 1.308651701997892 1103 74-75 1.5544504708469944 1103 76-77 1.3045181245886575 1103 78-79 1.553482054000309 1103 80-81 1.530158889572423 1103 82-83 1.6660309333844765 1103 84-85 1.4559564376367433 1103 86-87 1.4470777416074831 1103 88-89 1.6700590800797954 1103 90-91 1.5289643591450641 1103 92-93 1.464659992581506 1103 94-95 1.4904965271619233 1103 96-97 1.389021516187558 1103 98-99 1.6021556300134172 1103 100-101 1.6375491443623318 1104 1 0.23230660583940477 1104 2 0.1358720368296602 1104 3 0.24503618364913393 1104 4 0.29708094786995787 1104 5 0.23689937550616946 1104 6 0.18689505497830083 1104 7 0.27787454973335457 1104 8 0.22607642996355537 1104 9 0.13239492233659433 1104 10-11 0.20227870001134818 1104 12-13 0.252056963616603 1104 14-15 -0.07321313030659837 1104 16-17 0.16352257886669008 1104 18-19 0.38811492509624657 1104 20-21 0.3785938708453074 1104 22-23 0.5016149643702619 1104 24-25 0.31374137443773975 1104 26-27 0.17272402452622515 1104 28-29 0.16009403974944547 1104 30-31 0.37692674240042123 1104 32-33 0.5034356695642117 1104 34-35 0.3070390367917284 1104 36-37 0.48889256884101684 1104 38-39 0.4019336856964344 1104 40-41 0.578326680726029 1104 42-43 0.24496265202986223 1104 44-45 0.1810911263181083 1104 46-47 0.44662474197971136 1104 48-49 0.24624042698042103 1104 50-51 0.3021907419840133 1104 52-53 0.1375606691988338 1104 54-55 0.14124418182946386 1104 56-57 0.07774287451785966 1104 58-59 0.44216073393737076 1104 60-61 0.48750873949321516 1104 62-63 -0.0017366109319922884 1104 64-65 -0.14602997547152796 1104 66-67 -0.06986901476302876 1104 68-69 0.0522221982702149 1104 70-71 0.1484062903582597 1104 72-73 0.40520020946057755 1104 74-75 0.9314258878794774 1104 76-77 0.6907944634824226 1104 78-79 0.8380244157211187 1104 80-81 0.7144214005469642 1104 82-83 0.4519999851843046 1104 84-85 0.43241605133297156 1104 86-87 0.5192902086838664 1104 88-89 0.3937201863133346 1104 90-91 0.8740861765287349 1104 92-93 0.7814400628185574 1104 94-95 0.5785946395412047 1104 96-97 0.6926595758890493 1104 98-99 1.164106903059949 1104 100-101 1.278599407751269 1105 1 0.08133428900036677 1105 2 0.18284670704704098 1105 3 -0.037276320221558024 1105 4 0.06843166317310789 1105 5 2.4550791466992905E-4 1105 6 0.05937910774351707 1105 7 0.07820897718118403 1105 8 0.10644120360571918 1105 9 0.03734630649464066 1105 10-11 0.02133177488593674 1105 12-13 0.05512409683020536 1105 14-15 0.16768301139155284 1105 16-17 -0.07741226971199922 1105 18-19 -0.10658997364654965 1105 20-21 -0.12145257741133975 1105 22-23 -0.11788091721764715 1105 24-25 0.026575027747945512 1105 26-27 0.32178422146683516 1105 28-29 -0.22683542083147046 1105 30-31 -0.29391768690533127 1105 32-33 -0.10254376934084064 1105 34-35 0.022953757792535612 1105 36-37 0.1184909462485777 1105 38-39 0.28727616417540247 1105 40-41 0.06925378792860215 1105 42-43 0.15927335990129166 1105 44-45 0.38321845647231356 1105 46-47 -0.1523100446679635 1105 48-49 0.1239266844895539 1105 50-51 0.19442304519822784 1105 52-53 0.39846052675751054 1105 54-55 0.3957341799715266 1105 56-57 0.2060716662438793 1105 58-59 0.19461475016328933 1105 60-61 0.237477773988779 1105 62-63 0.2691031735480962 1105 64-65 0.12555298111482927 1105 66-67 0.1497770495213615 1105 68-69 0.08235163407872648 1105 70-71 0.0655503418848582 1105 72-73 -0.08800286231746313 1105 74-75 0.13527180352944157 1105 76-77 -0.37319575141818007 1105 78-79 0.1431894618597198 1105 80-81 -0.20702685609513694 1105 82-83 0.21409201466346417 1105 84-85 0.5663573407365732 1105 86-87 0.5019959744607938 1105 88-89 0.11059483795141034 1105 90-91 0.20611379775868244 1105 92-93 0.5490052452053789 1105 94-95 0.318213454181695 1105 96-97 -0.06209362904065685 1105 98-99 0.39073568859286567 1105 100-101 0.7162271404570362 1106 1 0.19311890379662344 1106 2 0.2364635493133278 1106 3 0.21501348607708337 1106 4 0.19734919190324973 1106 5 0.14444115967289406 1106 6 0.21227506735878876 1106 7 0.2712028391617949 1106 8 0.16302188701011744 1106 9 0.3595253989856104 1106 10-11 0.24101932866531683 1106 12-13 0.20017065781841126 1106 14-15 0.21836469485331378 1106 16-17 0.11789530051191832 1106 18-19 0.0029096152400001074 1106 20-21 0.47603867447691783 1106 22-23 0.40659467411646233 1106 24-25 0.34803706189904915 1106 26-27 0.2291498447339464 1106 28-29 0.37553182776787963 1106 30-31 0.29169907887472846 1106 32-33 0.24783005707367067 1106 34-35 0.11251912483079707 1106 36-37 0.3332164563160873 1106 38-39 0.6091779941082862 1106 40-41 0.8429597366445947 1106 42-43 0.39341680981237204 1106 44-45 0.34807303704099013 1106 46-47 0.6584343574965956 1106 48-49 0.5974337690259546 1106 50-51 0.6426801153934605 1106 52-53 0.6380249377179936 1106 54-55 0.8172019505893502 1106 56-57 0.6961571395979931 1106 58-59 1.3790803982884299 1106 60-61 1.23565166541659 1106 62-63 1.435862261067463 1106 64-65 0.8361386743668362 1106 66-67 1.2946419557301283 1106 68-69 1.5365746296616436 1106 70-71 1.2283034634788308 1106 72-73 1.189556079878077 1106 74-75 1.2325814006382672 1106 76-77 0.7137499624851067 1106 78-79 1.2938673210103424 1106 80-81 1.2888333270893675 1106 82-83 1.193316444363063 1106 84-85 1.0400747623859985 1106 86-87 0.7413309267525108 1106 88-89 0.6991658840917232 1106 90-91 0.7877150368354933 1106 92-93 1.0439667152705852 1106 94-95 1.0257110161833403 1106 96-97 1.0873222074317965 1106 98-99 1.190475635434943 1106 100-101 1.539716059515488 1107 1 0.11746771018890456 1107 2 0.29105107724987533 1107 3 0.2513458370986257 1107 4 0.03835312774312172 1107 5 -0.15956785913606808 1107 6 -0.23793359148893956 1107 7 -0.19378005279050114 1107 8 0.03673878343226278 1107 9 0.17885254836951958 1107 10-11 -0.1236293135688129 1107 12-13 0.14736315796674404 1107 14-15 0.14264427793118273 1107 16-17 0.20138850673805564 1107 18-19 0.37017885437688136 1107 20-21 0.1384266810432493 1107 22-23 0.10788618338249023 1107 24-25 -0.008107001707287509 1107 26-27 -0.06820384684652225 1107 28-29 0.12468631442755651 1107 30-31 -0.0076818669135221285 1107 32-33 -0.2097953854610921 1107 34-35 -0.14380491939301265 1107 36-37 0.3711310545241844 1107 38-39 0.34643595973396657 1107 40-41 0.21502516256575888 1107 42-43 0.2741648171698259 1107 44-45 0.13482567247145738 1107 46-47 0.5063902919124459 1107 48-49 0.25017995679913696 1107 50-51 -0.2744299101202756 1107 52-53 -0.10299086497578713 1107 54-55 0.09453032621483004 1107 56-57 -0.1968631725002652 1107 58-59 -0.06643096422418182 1107 60-61 0.004435906234611764 1107 62-63 0.24791107339316198 1107 64-65 0.03212965555787406 1107 66-67 -0.15646660620496533 1107 68-69 -0.2494561054899478 1107 70-71 -0.33742901807809744 1107 72-73 0.060395264229651247 1107 74-75 -0.031228650586989204 1107 76-77 -0.24820688172288286 1107 78-79 0.0905386684916003 1107 80-81 0.290257349557276 1107 82-83 0.3521345057147016 1107 84-85 0.524578629002562 1107 86-87 0.21935746894906671 1107 88-89 0.11877783796921904 1107 90-91 0.5190000193318838 1107 92-93 0.7892150291884263 1107 94-95 0.881914414059171 1107 96-97 0.9900140054579438 1107 98-99 0.2835018809601486 1107 100-101 0.6292415831404199 1108 1 0.15162281548883527 1108 2 -0.017442784412963874 1108 3 0.003696371084053851 1108 4 0.0752829610598198 1108 5 -0.10449250231403084 1108 6 0.11803007753990613 1108 7 0.14930807975765248 1108 8 0.007454423715721248 1108 9 0.012488639795812162 1108 10-11 0.1944862668284273 1108 12-13 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68-69 -0.6433185031307573 2316 70-71 0.08644996767718993 2316 72-73 -0.20841118143768966 2316 74-75 -0.3470742494705803 2316 76-77 0.0198623144118244 2316 78-79 -0.16192980092570863 2316 80-81 -0.26404897762353485 2316 82-83 0.1893866713852006 2316 84-85 -0.3901505634458964 2316 86-87 -0.6472925218353147 2316 88-89 -0.723977368855607 2316 90-91 -0.4001790006528445 2316 92-93 -0.6361384556321354 2316 94-95 -0.2924451004093491 2316 96-97 -0.3696057844259393 2316 98-99 -0.42204592177293776 2316 100-101 -0.46005776650017083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 0.0 5 1.0 6 8.0 7 17.0 8 40.0 9 83.0 10 191.0 11 380.0 12 622.0 13 975.0 14 1168.0 15 1690.0 16 2481.0 17 3863.0 18 3259.0 19 1474.0 20 1108.0 21 1065.0 22 1070.0 23 1195.0 24 1362.0 25 1546.0 26 1736.0 27 2113.0 28 2548.0 29 3164.0 30 4015.0 31 5231.0 32 6663.0 33 9116.0 34 13070.0 35 19554.0 36 31142.0 37 51718.0 38 43122.0 39 2990.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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19.248251668706576 18-19 17.38424193339233 30.93987382808679 34.103726689704914 17.572157548815973 20-21 18.89097936795743 29.987100707754816 32.873175159648646 18.248744764639106 22-23 18.651463515622513 29.0983214956843 32.302904281118764 19.947310707574427 24-25 19.05831279905531 29.332050083160983 33.40750701350826 18.20213010427545 26-27 19.778539690629003 28.455397384065506 34.02284676830392 17.743216157001573 28-29 18.388532220710616 28.191927418657663 35.59179364913255 17.827746711499174 30-31 18.735837724207595 29.832009245861656 34.136203554560595 17.295949475370154 32-33 18.235406533104612 30.498514376197257 33.172182206185475 18.093896884512656 34-35 19.01938748117444 28.459466468894036 33.34933410986391 19.171811940067613 36-37 19.857116854750636 28.854432107753915 33.337732071350565 17.950718966144883 38-39 19.775294192597176 28.386741538265515 34.31427869344813 17.52368557568918 40-41 18.44865830214649 28.867604736608616 35.29205673920215 17.391680222042748 42-43 18.206077868423566 30.204385274432276 33.47855365113454 18.110983206009617 44-45 18.81896069268954 30.2082527607027 32.16884080061516 18.8039457459926 46-47 19.18736918736919 29.924697424697428 30.47251797251797 20.415415415415417 48-49 19.89052340369381 29.83214498596279 31.054770149199403 19.222561461144 50-51 20.928010265721092 30.30913198686752 30.542112891079153 18.220744856332235 52-53 19.433663510494537 30.695101032391335 31.548678002193093 18.322557454921036 54-55 18.728983614613526 30.066342386778842 31.807716283916292 19.396957714691336 56-57 20.04982538364408 31.640711206161054 29.411764705882355 18.897698704312514 58-59 19.24829545842329 30.28612638518886 30.503161108469584 19.96241704791826 60-61 19.09097527460027 30.11798483897094 30.64488975037993 20.146150136048853 62-63 21.260858288246883 29.767977321023103 30.34723770607427 18.623926684655743 64-65 21.030480341794785 29.725044476092112 31.00063244775481 18.243842734358292 66-67 19.290183141849617 30.467296098282333 30.907064042771015 19.33545671709703 68-69 19.22968422968423 31.03967603967604 30.884065884065887 18.846573846573847 70-71 19.292290682251984 31.107996878689775 30.276481655477422 19.323230783580815 72-73 19.34607632142906 29.678862140039403 30.001683494023595 20.973378044507943 74-75 19.45686842811708 30.673943607500192 30.000090999676953 19.869096964705776 76-77 20.55978451276498 29.476160359630722 30.555189029078946 19.40886609852535 78-79 19.220837722301724 29.068886517771624 31.43176301163238 20.278512748294276 80-81 19.42230645150282 31.329955567284962 29.73624758554947 19.511490395662747 82-83 19.051432861969715 31.56118815485603 29.228563952628527 20.158815030545725 84-85 18.883357526722698 29.600194760576453 29.782441514946058 21.734006197754795 86-87 19.470305048960725 30.731043646026716 29.438999308361545 20.359651996651014 88-89 20.650784189716127 28.676955696807276 30.225542699323416 20.446717414153177 90-91 18.872594742900823 28.822589965374306 31.736653638427693 20.568161653297174 92-93 18.786243547539478 30.10890411114726 30.59871098664349 20.506141354669776 94-95 18.361232317625273 30.15661044403292 29.912731309803853 21.569425928537953 96-97 18.71298729367869 29.880899566597275 28.930143671296456 22.47596946842758 98-99 19.049059934841566 29.747875070527634 29.242351163933535 21.96071383069727 100-101 18.70570865022147 30.675150889182618 29.29172515305043 21.327415307545483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 770.0 1 491.0 2 201.0 3 176.0 4 150.5 5 137.5 6 173.5 7 262.0 8 332.5 9 386.0 10 364.5 11 320.5 12 338.5 13 374.5 14 431.0 15 446.5 16 466.5 17 493.0 18 528.0 19 615.5 20 693.5 21 798.5 22 933.0 23 1095.0 24 1272.5 25 1589.5 26 2086.0 27 2656.5 28 3138.5 29 3528.0 30 4121.5 31 4786.0 32 5877.0 33 7053.0 34 8149.5 35 9148.0 36 10332.0 37 11402.5 38 11270.0 39 10437.0 40 9438.0 41 8245.0 42 7083.0 43 6880.5 44 7053.0 45 6766.0 46 6397.0 47 7403.5 48 15929.0 49 15460.5 50 6129.0 51 3760.0 52 2610.5 53 2068.0 54 1667.0 55 1359.5 56 1041.0 57 821.5 58 645.0 59 467.5 60 348.0 61 267.5 62 174.5 63 103.5 64 63.0 65 47.0 66 38.0 67 25.0 68 19.0 69 13.0 70 8.0 71 5.0 72 2.0 73 0.0 74 0.0 75 0.0 76 0.0 77 1.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03139488854814566 2 0.007279974156091745 3 9.099967695114682E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.016607441043584293 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.824975771336011E-4 20-21 6.824975771336011E-4 22-23 9.099967695114682E-4 24-25 0.012057457196026954 26-27 0.005687479809446677 28-29 0.0 30-31 0.003184988693290139 32-33 0.0036399870780458726 34-35 0.0 36-37 0.009554966079870417 38-39 0.012284956388404822 40-41 0.0015924943466450695 42-43 0.0 44-45 0.0 46-47 4.549983847557341E-4 48-49 0.0036399870780458726 50-51 0.00932746688749255 52-53 0.0 54-55 0.005459980617068809 56-57 0.006142478194202411 58-59 2.2749919237786704E-4 60-61 0.003184988693290139 62-63 0.008189970925603215 64-65 0.0 66-67 0.0027299903085344045 68-69 4.549983847557341E-4 70-71 6.824975771336011E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.002957489500912272 80-81 0.004777483039935209 82-83 0.012967453965538422 84-85 0.010919961234137618 86-87 0.005914979001824544 88-89 0.0 90-91 9.099967695114682E-4 92-93 2.2749919237786704E-4 94-95 0.0 96-97 0.00386748627042374 98-99 0.004094985462801608 100-101 2.2749919237786704E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 219781.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.563275999544004 #Duplication Level Percentage of deduplicated Percentage of total 1 81.2489999999995 37.01970611686928 2 10.083999999999937 9.189201503587977 3 2.885999999999982 3.9448684360404953 4 1.3669999999999916 2.491399931655051 5 0.7800000000006163 1.7769677639836203 6 0.5309999999999983 1.4516459733454672 7 0.39799999999999747 1.269392869347288 8 0.2859999999999982 1.0424877548695604 9 0.2549999999999984 1.0456771841895285 >10 1.8549999999999882 16.96320765463013 >50 0.1969999999999988 6.064016402779274 >100 0.09299999999999942 7.1994532406879035 >500 0.011999999999999924 4.645631620913478 >1k 0.006999999999999956 5.896343547100953 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2938 1.3367852544123469 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2819 1.2826404466264145 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1906 0.8672269213444291 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1767 0.8039821458633822 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1476 0.6715776158994635 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1032 0.46955833306791767 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1003 0.4563633799100013 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 993 0.451813396062444 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 976 0.44407842352159654 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 949 0.4317934671331917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 903 0.4108635414344279 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 901 0.40995354466491646 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 894 0.40676855597162626 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 888 0.4040385656630919 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 861 0.3917536092746871 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 805 0.36627369972836593 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 802 0.36490870457409874 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 660 0.30029893393878454 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 564 0.25661908900223407 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 453 0.20611426829434754 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 427 0.19428431029069848 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 422 0.1920093183669198 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 402 0.1829093506718051 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 338 0.15378945404743813 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 315 0.14332449119805624 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 304 0.13831950896574316 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 302 0.1374095121962317 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 295 0.13422452350294156 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 291 0.13240452996391863 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 264 0.1201195735755138 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 260 0.11829958003649087 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 254 0.11556958972795646 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 245 0.11147460426515485 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 235 0.10692462041759752 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.5499838475573413E-4 0.0 24-25 0.0 0.0 0.0 0.0025024911161565374 0.0 26-27 0.0 0.0 0.0 0.005232481424690943 0.0 28-29 0.0 0.0 0.0 0.0193374313521187 0.0 30-31 0.0 0.0 0.0 0.04777483039935208 0.0 32-33 0.0 0.0 0.0 0.08508469794932227 0.0 34-35 0.0 0.0 0.0 0.11511459134320073 0.0 36-37 0.0 0.0 0.0 0.12990203884776208 0.0 38-39 0.0 0.0 0.0 0.14286949281330052 0.0 40-41 0.0 0.0 0.0 0.16470941528157576 0.0 42-43 0.0 0.0 0.0 0.18745933451936245 0.0 44-45 0.0 0.0 0.0 0.20861675941050412 0.0 46-47 0.0 0.0 0.0 0.24023914715102762 0.0 48-49 0.0 0.0 0.0 0.28619398401135676 0.0 50-51 0.0 0.0 0.0 0.32759883702412856 0.0 52-53 0.0 0.0 0.0 0.4110910406268058 0.0 54-55 0.0 0.0 0.0 0.5257506335852508 0.0 56-57 0.0 0.0 0.0 0.669302623975685 0.0 58-59 0.0 0.0 0.0 0.8460694964532876 0.0 60-61 0.0 0.0 0.0 1.1238460103466634 0.0 62-63 0.0 0.0 0.0 1.2967453965538422 0.0 64-65 0.0 0.0 0.0 1.428922427325383 0.0 66-67 0.0 0.0 0.0 1.7330888475345914 0.0 68-69 0.0 0.0 0.0 2.1346249220815263 0.0 70-71 0.0 0.0 0.0 2.450848799486762 0.0 72-73 0.0 0.0 0.0 2.7249853263020913 0.0 74-75 0.0 0.0 0.0 3.3374131521833097 0.0 76-77 0.0 0.0 0.0 3.713469317183924 0.0 78-79 0.0 0.0 0.0 3.948248483717883 0.0 80-81 0.0 0.0 0.0 4.188032632484155 0.0 82-83 0.0 0.0 0.0 4.640073527738977 0.0 84-85 0.0 0.0 0.0 5.252501353620195 0.0 86-87 0.0 0.0 0.0 6.076503428412829 0.0 88-89 0.0 0.0 0.0 7.2321993256923935 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 50 0.0016090451 38.018852 9 TGTAGAT 65 1.3208282E-4 36.55659 5 ACTGCCT 70 0.0083568115 27.156324 8 CAAGGGT 70 0.008384705 27.137787 4 TAGATAT 90 8.8986167E-4 26.401981 7 CTGGTCG 150 1.0490421E-6 25.345901 9 TGGAACG 115 1.2728511E-4 24.794905 5 GGACAAT 115 1.2728511E-4 24.794905 6 GAGTACT 1460 0.0 24.070845 12-13 GTAGATA 100 0.0016446491 23.761784 6 ACAGCAA 120 1.6989613E-4 23.761782 8 AGTACTT 1545 0.0 23.36133 12-13 GGAACGG 125 2.2403068E-4 22.811312 6 CTTTAGG 125 2.2506955E-4 22.79574 2 GTACTTT 1555 0.0 22.447575 14-15 CAATGGG 130 2.9212178E-4 21.933954 9 ACCCTGG 195 4.169433E-7 21.933952 6 ATAACCC 195 4.1969543E-7 21.918982 3 AGATATA 110 0.0028617913 21.601622 8 GTATAGT 110 0.002873159 21.586876 1 >>END_MODULE