##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936944_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 228549 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.279358037007384 33.0 31.0 34.0 31.0 34.0 2 32.4217038796932 34.0 31.0 34.0 31.0 34.0 3 32.522649409973354 34.0 31.0 34.0 31.0 34.0 4 36.14509361231071 37.0 35.0 37.0 35.0 37.0 5 36.009149022747856 37.0 35.0 37.0 35.0 37.0 6 36.1281081956167 37.0 35.0 37.0 35.0 37.0 7 36.08957816485743 37.0 35.0 37.0 35.0 37.0 8 36.07968969455128 37.0 35.0 37.0 35.0 37.0 9 37.78476825538506 39.0 37.0 39.0 35.0 39.0 10-11 37.71106196045487 39.0 37.0 39.0 35.0 39.0 12-13 37.63441537700887 39.0 37.0 39.0 35.0 39.0 14-15 38.785019842572055 40.0 38.0 41.0 35.0 41.0 16-17 38.76906702720204 40.0 38.0 41.0 35.0 41.0 18-19 38.972710447212634 40.0 38.0 41.0 35.0 41.0 20-21 39.01153144402295 40.0 38.0 41.0 35.0 41.0 22-23 38.94093826706745 40.0 38.0 41.0 35.0 41.0 24-25 38.863867266975575 40.0 38.0 41.0 35.0 41.0 26-27 38.59443051599438 40.0 38.0 41.0 34.5 41.0 28-29 38.43012876888545 40.0 38.0 41.0 34.0 41.0 30-31 38.39931918319485 40.0 38.0 41.0 34.5 41.0 32-33 38.22447702680826 40.0 37.0 41.0 34.0 41.0 34-35 38.095056202389856 40.0 37.0 41.0 34.0 41.0 36-37 37.912664242678815 40.0 37.0 41.0 34.0 41.0 38-39 37.67102678200298 40.0 37.0 41.0 33.0 41.0 40-41 37.280309692888615 40.0 36.5 41.0 33.0 41.0 42-43 37.095817527094844 40.0 36.0 41.0 33.0 41.0 44-45 36.82248445628727 40.0 36.0 41.0 31.5 41.0 46-47 36.281366359073985 40.0 36.0 41.0 30.5 41.0 48-49 35.79991817947136 39.0 36.0 41.0 29.0 41.0 50-51 35.47915983005832 39.0 36.0 40.5 27.0 41.0 52-53 35.16998980524964 39.0 35.0 40.0 26.0 41.0 54-55 34.81960542378221 39.0 35.0 40.0 24.0 41.0 56-57 34.707780388450615 39.0 35.0 40.0 24.0 41.0 58-59 34.473467396488275 38.0 35.0 40.0 23.0 41.0 60-61 34.12275923324977 38.0 34.0 40.0 22.0 41.0 62-63 33.69981491933896 38.0 34.0 40.0 20.5 41.0 64-65 33.31508998070436 37.0 33.0 40.0 18.5 41.0 66-67 33.017333263326464 37.0 33.0 39.0 17.5 40.5 68-69 32.60546316107268 36.0 32.5 39.0 12.5 40.0 70-71 32.13273521214269 36.0 32.0 38.5 8.0 40.0 72-73 31.708222306813855 35.0 31.5 38.0 7.0 40.0 74-75 31.20701250060162 35.0 31.0 37.0 2.0 39.0 76-77 29.710073113424258 33.5 29.5 36.0 2.0 38.5 78-79 30.278531080862308 34.0 30.0 36.0 2.0 39.0 80-81 30.152945320259548 34.0 31.0 36.0 2.0 37.5 82-83 29.79957470826825 34.0 30.5 35.5 2.0 37.0 84-85 29.39677268331955 34.0 30.0 35.0 2.0 37.0 86-87 29.023443550398383 34.0 29.0 35.0 2.0 36.0 88-89 28.568296076552514 34.0 29.0 35.0 2.0 36.0 90-91 28.101431202936787 34.0 29.0 35.0 2.0 35.0 92-93 27.72287561967018 33.5 28.0 35.0 2.0 35.0 94-95 27.279530866466274 33.0 27.0 35.0 2.0 35.0 96-97 26.587978945434024 33.0 25.5 34.0 2.0 35.0 98-99 25.858706448070215 32.0 24.5 34.0 2.0 35.0 100-101 22.97535101881872 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.015155016878438232 1101 2 0.12526408176160686 1101 3 0.01596839924444282 1101 4 -0.0019984111549362638 1101 5 -0.18429856325769833 1101 6 0.004270620317065266 1101 7 -0.021282688969932906 1101 8 0.14370599115485305 1101 9 -0.17192467594159666 1101 10-11 0.1982111982770789 1101 12-13 0.15914125311794436 1101 14-15 0.2557796444605316 1101 16-17 0.344475895289186 1101 18-19 -0.005115893711469255 1101 20-21 -0.1858788885435061 1101 22-23 0.062024975129844506 1101 24-25 -0.19654269359534027 1101 26-27 -0.01006104493972515 1101 28-29 0.10792400532485402 1101 30-31 0.37467675193320105 1101 32-33 0.5724328273012702 1101 34-35 0.31793736249728255 1101 36-37 0.5454305012247147 1101 38-39 0.4340894348535542 1101 40-41 0.4509008615222214 1101 42-43 0.5235930948409759 1101 44-45 0.4410532408417325 1101 46-47 0.4315021894143527 1101 48-49 0.7020409166906134 1101 50-51 0.6665555122117226 1101 52-53 0.5743243925836907 1101 54-55 0.53671204508057 1101 56-57 1.0120576672627237 1101 58-59 0.7827057186549453 1101 60-61 0.8157269998870476 1101 62-63 0.6693935973712755 1101 64-65 0.745555132731397 1101 66-67 0.8876265339143075 1101 68-69 0.39241923066047946 1101 70-71 0.41627139693120796 1101 72-73 0.3806462647419657 1101 74-75 0.025376682979352694 1101 76-77 0.16376266489244173 1101 78-79 0.2737786773083499 1101 80-81 0.5475089331836962 1101 82-83 1.0340666565432244 1101 84-85 0.7961592088531297 1101 86-87 0.29215502687409867 1101 88-89 0.7354671492768858 1101 90-91 0.41724869362799666 1101 92-93 0.6779360067002322 1101 94-95 0.7150472424512877 1101 96-97 0.09963508245495589 1101 98-99 0.22914001968979392 1101 100-101 0.59641047722479 1102 1 -0.1438382374722238 1102 2 -0.2536940271891339 1102 3 -0.3123689965947918 1102 4 -0.08474882212937018 1102 5 -0.12921098318854263 1102 6 -0.08227776129395181 1102 7 -0.162975336779013 1102 8 0.1567324635770504 1102 9 0.05689455199333082 1102 10-11 -0.021793336613228576 1102 12-13 0.1715611839608684 1102 14-15 0.0038494817713399243 1102 16-17 -0.03755460184816428 1102 18-19 0.029661046371280975 1102 20-21 -0.0425876918993211 1102 22-23 -0.24993012945606097 1102 24-25 -0.16514424679527195 1102 26-27 -0.2748702961159637 1102 28-29 -0.1200959481925139 1102 30-31 0.09003302425336557 1102 32-33 -0.06807508041371335 1102 34-35 0.0011866114060765653 1102 36-37 -0.05073184784846774 1102 38-39 0.2986095867723719 1102 40-41 0.30634556420347536 1102 42-43 -0.004472207579524934 1102 44-45 -0.14953686176016134 1102 46-47 -0.11800747557062863 1102 48-49 -0.06910584369711614 1102 50-51 -0.21782745927515634 1102 52-53 -0.16474368745712553 1102 54-55 0.01954748524835992 1102 56-57 -0.07645772302127796 1102 58-59 -0.23963995335911648 1102 60-61 -0.12622640411498764 1102 62-63 0.14029660740472139 1102 64-65 -0.0013413017110934788 1102 66-67 -0.13830737005938687 1102 68-69 0.20628465779046223 1102 70-71 -0.12488840126617617 1102 72-73 -0.34794621867733966 1102 74-75 0.03143643015921782 1102 76-77 0.173149887838985 1102 78-79 -0.10799106363604238 1102 80-81 0.1661076520771836 1102 82-83 0.1456646385170366 1102 84-85 -0.12122075826891887 1102 86-87 0.21520927549945768 1102 88-89 0.38191009468814485 1102 90-91 0.3851700132810798 1102 92-93 0.2668255254349994 1102 94-95 0.1042825465723709 1102 96-97 0.7757248732831421 1102 98-99 0.5452898978146159 1102 100-101 0.8086263380036556 1103 1 0.09464902183827917 1103 2 0.09383278014396979 1103 3 0.08629351925900153 1103 4 0.0957423573932843 1103 5 -0.010613035534902338 1103 6 0.023268185898480453 1103 7 -0.14287848447877138 1103 8 0.018037663623175604 1103 9 0.03599059719107345 1103 10-11 -0.022882924990952347 1103 12-13 0.03143665957483677 1103 14-15 0.07627540129169574 1103 16-17 0.09065363059735176 1103 18-19 -0.055459442502233 1103 20-21 0.03210964466242672 1103 22-23 -0.016320075264886214 1103 24-25 0.041393283471073516 1103 26-27 -0.08326992715482362 1103 28-29 -0.17169285814294 1103 30-31 -0.15495717521625352 1103 32-33 0.20614952468256575 1103 34-35 0.22932566363714102 1103 36-37 0.4571316365283309 1103 38-39 0.6434153056701035 1103 40-41 0.4409356714328041 1103 42-43 -0.0707064979058245 1103 44-45 0.1994542601123115 1103 46-47 0.36345222695445045 1103 48-49 0.5162913660342312 1103 50-51 0.7911602035865997 1103 52-53 0.6035544792671956 1103 54-55 0.918244591209394 1103 56-57 0.540918040843593 1103 58-59 0.7266173970386731 1103 60-61 0.45655925252054885 1103 62-63 0.9539020495815862 1103 64-65 1.0179426830692577 1103 66-67 1.141238346532326 1103 68-69 1.3919528233617697 1103 70-71 1.6567384867771793 1103 72-73 1.4740357944669036 1103 74-75 1.1031301924098749 1103 76-77 1.4221352446935924 1103 78-79 1.6639909495082001 1103 80-81 1.819560897782246 1103 82-83 1.7297358941379315 1103 84-85 1.2973195392060042 1103 86-87 1.2067001091210443 1103 88-89 1.5050278163530777 1103 90-91 1.2379833420023978 1103 92-93 1.0075380816439186 1103 94-95 1.3329231553582588 1103 96-97 1.1181150496926868 1103 98-99 1.0185286848548571 1103 100-101 2.124912513490802 1104 1 0.015038799370913125 1104 2 0.24137828483232937 1104 3 0.2376080991450138 1104 4 0.17516872253393245 1104 5 -0.020173616524054694 1104 6 0.18065005756606922 1104 7 0.17565433405404463 1104 8 0.1453588623729445 1104 9 -0.09077902957855599 1104 10-11 0.21167305956502958 1104 12-13 0.4241559094369407 1104 14-15 0.4075403396994872 1104 16-17 0.40436019430391923 1104 18-19 0.3852006053006747 1104 20-21 0.44620230875508327 1104 22-23 0.33126220088881553 1104 24-25 0.218999461077253 1104 26-27 0.2633470980336341 1104 28-29 0.3505538783895048 1104 30-31 0.26773081890031136 1104 32-33 0.3156825270727097 1104 34-35 0.2951006222692456 1104 36-37 0.3671334751015962 1104 38-39 0.5878903930023327 1104 40-41 0.4241127260384374 1104 42-43 0.1826948626383853 1104 44-45 0.4130964737545284 1104 46-47 0.6938438430603568 1104 48-49 0.7951569663284062 1104 50-51 1.0953981149673666 1104 52-53 1.036676376674805 1104 54-55 1.3025983327059905 1104 56-57 1.0772298628697001 1104 58-59 1.0301198791125827 1104 60-61 1.1757171859641886 1104 62-63 1.2221498892580058 1104 64-65 1.5202868639848717 1104 66-67 1.5169507936733524 1104 68-69 1.4866393466197252 1104 70-71 1.3297022668838139 1104 72-73 1.325249252823216 1104 74-75 1.1909220659160376 1104 76-77 1.3324588335886105 1104 78-79 1.6551657978259051 1104 80-81 1.6628225267581591 1104 82-83 1.6642367214958966 1104 84-85 1.4514839076560868 1104 86-87 1.330803804653307 1104 88-89 1.8706281105248053 1104 90-91 1.941835152997065 1104 92-93 2.2077587104359786 1104 94-95 2.356916536494495 1104 96-97 2.53965393551729 1104 98-99 2.401503496401098 1104 100-101 2.3166297409223127 1105 1 0.04859983293194858 1105 2 0.06487760833164913 1105 3 0.10905647059338719 1105 4 0.1756307229959333 1105 5 0.03290693655649335 1105 6 0.15217102908704305 1105 7 0.07384923224894635 1105 8 0.16804638506046388 1105 9 0.16419572539619764 1105 10-11 0.11478496267693572 1105 12-13 0.2848380201190537 1105 14-15 0.07815051030965492 1105 16-17 -0.2585527186089962 1105 18-19 -0.07098360088352962 1105 20-21 0.09895946151223711 1105 22-23 0.17768829731490854 1105 24-25 0.08077057284835831 1105 26-27 0.24718533187186864 1105 28-29 -0.10658732875170074 1105 30-31 -0.06874576757627437 1105 32-33 -0.13882692743674596 1105 34-35 -0.3986178214492071 1105 36-37 -0.4224253144571861 1105 38-39 -0.6875196324076924 1105 40-41 -0.3892143372886281 1105 42-43 -0.4845823046387778 1105 44-45 -0.9473711367130804 1105 46-47 -1.0838266846101732 1105 48-49 -1.0028085816371473 1105 50-51 -0.9029353833661347 1105 52-53 -0.9354303454319179 1105 54-55 -0.8042392741316107 1105 56-57 -0.9289493933095514 1105 58-59 -0.7957966968039969 1105 60-61 -0.8989701387231293 1105 62-63 -1.04901963191152 1105 64-65 -1.078952485254483 1105 66-67 -1.2371412104186525 1105 68-69 -0.9998141398337594 1105 70-71 -1.3526806654991184 1105 72-73 -1.4743332967593332 1105 74-75 -1.1234527484587744 1105 76-77 -1.0969210957913198 1105 78-79 -1.498053355393246 1105 80-81 -1.6820525681169336 1105 82-83 -1.4110281037689276 1105 84-85 -1.5353160791439002 1105 86-87 -1.3749761511266456 1105 88-89 -1.2612812713845791 1105 90-91 -1.9487862376243257 1105 92-93 -1.947110444433175 1105 94-95 -1.5583641287861703 1105 96-97 -1.0023716065082553 1105 98-99 -0.804862209964611 1105 100-101 -0.3696801953876232 1106 1 -0.07972027745093868 1106 2 0.044276634815879845 1106 3 0.08174820337480071 1106 4 0.03470090045176022 1106 5 -0.20328562026386265 1106 6 -0.08139610134107755 1106 7 0.19760838291392702 1106 8 0.22896777557826908 1106 9 0.20714156879047607 1106 10-11 0.18419587065286436 1106 12-13 0.22467359560485534 1106 14-15 0.26668295444748935 1106 16-17 0.30734165109703326 1106 18-19 0.08647490971399208 1106 20-21 0.19146262352212773 1106 22-23 0.31759286210736803 1106 24-25 0.11282185489964291 1106 26-27 0.31614589298489904 1106 28-29 0.36329692252269297 1106 30-31 0.42656870867990193 1106 32-33 0.12379236657268677 1106 34-35 0.03144426892679064 1106 36-37 0.36610329694137533 1106 38-39 0.22841881379981288 1106 40-41 0.3870782540263846 1106 42-43 0.6702028703257668 1106 44-45 0.7739791373277782 1106 46-47 0.877683031383846 1106 48-49 1.0976570961827932 1106 50-51 1.142041811835277 1106 52-53 1.251559421154262 1106 54-55 1.423674565889847 1106 56-57 1.1123771177478972 1106 58-59 1.1919097920235728 1106 60-61 0.947346598849208 1106 62-63 0.5950051275840877 1106 64-65 0.8448622401297499 1106 66-67 1.146103969911728 1106 68-69 0.8799336659230121 1106 70-71 1.0162683973961322 1106 72-73 0.9601939348530983 1106 74-75 0.6059366961773058 1106 76-77 0.36831595913183435 1106 78-79 0.734229807383187 1106 80-81 0.47237807846618196 1106 82-83 0.3883567415800435 1106 84-85 0.5566313356734689 1106 86-87 0.006141858915128751 1106 88-89 0.014896337752670519 1106 90-91 0.3037912795271822 1106 92-93 0.39419498655835383 1106 94-95 0.11936957249013247 1106 96-97 0.5322955200871213 1106 98-99 0.8996335931859001 1106 100-101 1.6702665295366792 1107 1 0.2709075252396431 1107 2 0.21842461687866432 1107 3 0.3308513423882573 1107 4 0.2108443982096091 1107 5 0.13299240664196788 1107 6 -0.09778623587022395 1107 7 0.10855008694979773 1107 8 0.188003650017734 1107 9 0.25765726385773746 1107 10-11 0.008545646437617904 1107 12-13 0.1731713534523891 1107 14-15 0.15562914278829254 1107 16-17 0.30172505916878123 1107 18-19 0.1875847751848454 1107 20-21 0.16889535894813434 1107 22-23 0.0387139383405497 1107 24-25 0.1303218548996412 1107 26-27 0.41462122930776246 1107 28-29 0.24939557723121197 1107 30-31 0.2496516683211638 1107 32-33 0.10239102128120692 1107 34-35 0.4283052554738731 1107 36-37 0.19990374537186995 1107 38-39 0.37572823084017415 1107 40-41 0.6293540387797449 1107 42-43 0.31340914835267597 1107 44-45 0.18707330773136022 1107 46-47 0.7528399820564928 1107 48-49 0.25161449528592783 1107 50-51 0.2047996593689092 1107 52-53 0.6430952955937244 1107 54-55 0.5700983327059888 1107 56-57 0.5650677007075373 1107 58-59 0.7306990296917277 1107 60-61 0.5647905450375532 1107 62-63 0.6677966074047248 1107 64-65 0.7516671728651758 1107 66-67 0.7677519519745104 1107 68-69 -0.0026560201756424817 1107 70-71 0.1943065139880602 1107 72-73 0.736841916915882 1107 74-75 0.8384703284643003 1107 76-77 0.5039549725847472 1107 78-79 0.5392970719571792 1107 80-81 0.29835341478905164 1107 82-83 0.8837154859746619 1107 84-85 0.8979317841039638 1107 86-87 1.3269041907536945 1107 88-89 1.0027999251966158 1107 90-91 0.9580513692132833 1107 92-93 1.004834000011268 1107 94-95 0.7014435635215222 1107 96-97 0.5780130088763613 1107 98-99 1.380586507984109 1107 100-101 1.5779907447833175 1108 1 -0.06274544279688854 1108 2 0.06200909176450864 1108 3 0.19774631955720423 1108 4 0.04262522012741954 1108 5 -0.17965599518451825 1108 6 -0.15844833632684896 1108 7 -0.03035402263924425 1108 8 0.14761552216385354 1108 9 0.06567096248787152 1108 10-11 0.05197030397186353 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64-65 -2.254967442519437 2316 66-67 -2.3824403557177973 2316 68-69 -2.6734252509448737 2316 70-71 -2.5097319475503994 2316 72-73 -2.6404657753918137 2316 74-75 -2.200375825381851 2316 76-77 -2.213641181261405 2316 78-79 -2.4745490818889735 2316 80-81 -2.6438581236724907 2316 82-83 -2.472726821717643 2316 84-85 -2.417260523588343 2316 86-87 -2.2566535015539984 2316 88-89 -2.6872962286495365 2316 90-91 -2.5534870923251773 2316 92-93 -2.954589076911809 2316 94-95 -2.503075667247707 2316 96-97 -1.8797754526620984 2316 98-99 -2.3417211843235854 2316 100-101 -3.1266246398320696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 17.0 7 27.0 8 63.0 9 91.0 10 229.0 11 395.0 12 660.0 13 1023.0 14 1261.0 15 1877.0 16 2680.0 17 3998.0 18 3439.0 19 1728.0 20 1117.0 21 1107.0 22 1163.0 23 1351.0 24 1469.0 25 1622.0 26 1892.0 27 2299.0 28 2715.0 29 3513.0 30 4357.0 31 5852.0 32 7390.0 33 9903.0 34 14373.0 35 20905.0 36 33326.0 37 54054.0 38 40426.0 39 2224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.91944610994647 22.260200531307305 16.3886858678384 19.431667490907817 2 14.387793501185818 24.28216370430658 40.1520123920292 21.178030402478406 3 18.91208372894853 29.105173115376704 33.11703938358412 18.86570377209064 4 11.340675303764183 20.10334764098727 41.81904099339748 26.73693606185107 5 11.03672490668907 40.45559362378958 36.3385359919838 12.169145477537555 6 25.50087727358247 41.22617031796245 18.805595299038718 14.467357109416362 7 22.92199922117358 37.799334059654605 23.44442548425064 15.834241234921176 8 22.704540383025083 35.26289767183405 21.99134540076745 20.041216544373416 9 24.541783162472818 19.436094666789177 23.875842817076425 32.146279353661576 10-11 21.06856735317153 28.869957864615465 29.610936823175777 20.45053795903723 12-13 25.3370935241725 27.19983197285312 29.004373519096678 18.45870098387771 14-15 22.849679379893807 28.5228284578512 29.325527311232314 19.30196485102268 16-17 18.080805429032722 31.762335429163986 31.083706338684486 19.073152803118806 18-19 17.315913138216647 30.84464027092896 34.22731429421519 17.612132296639203 20-21 18.79251012367178 30.25069077241811 32.80594709656812 18.150852007341985 22-23 18.70381146985084 29.101672741274225 32.42396531129265 19.77055047758229 24-25 19.087591719937517 29.388883784221324 33.42740506412192 18.096119431719238 26-27 19.92286118075336 28.347312830071452 33.99621087819242 17.733615110982765 28-29 18.33505798550417 28.436415027882482 35.49334167583686 17.735185310776487 30-31 18.520317306135663 29.932530879592562 34.154382648797416 17.392769165474363 32-33 18.214693566806243 30.54268450091665 33.246918603888005 17.995703328389098 34-35 18.93401415013848 28.538300320718967 33.40268388835654 19.125001640786003 36-37 19.850840308557977 28.711162255466053 33.45132511037117 17.9866723256048 38-39 19.91125561489838 28.406298750883398 34.20925383388434 17.473191800333883 40-41 18.5592586272528 28.883959238500278 35.138416707139385 17.418365427107535 42-43 18.114933777876953 30.16136583402246 33.44884466788304 18.27485572021755 44-45 18.828949844014577 30.24476365911607 32.03389237224729 18.89239412462207 46-47 19.10946886663254 29.973441143912243 30.567405676682025 20.349684312773192 48-49 19.787004099777292 29.76429562150786 31.13468009048388 19.31402018823097 50-51 20.952247602753964 30.210222561569246 30.654776880550095 18.182752955126706 52-53 19.504041829379013 30.55294851179733 31.710913486255592 18.232096172568067 54-55 18.699928680501774 30.138176058525744 31.815715667099838 19.346179593872648 56-57 19.976722664992334 31.730026668300166 29.393104745819816 18.900145920887688 58-59 19.327802790648832 30.172523178836926 30.43636156797886 20.063312462535386 60-61 19.19419977859746 30.286204346665617 30.485729162564596 20.033866712172326 62-63 21.2606487011538 29.63828325654455 30.410327763411786 18.690740278889866 64-65 21.04323589960118 29.7385456478603 31.009829423514045 18.208389029024474 66-67 19.31577035996657 30.45145679919842 31.044327474633448 19.18844536620156 68-69 19.146264359643578 31.026456091376666 31.03389433752573 18.793385211454027 70-71 19.3961867883044 31.11606996357431 30.11605902492917 19.371684223192116 72-73 19.363766036018692 29.770770253951028 30.01163869296603 20.85382501706425 74-75 19.56738615828232 30.626233367317024 30.005994548024205 19.80038592637645 76-77 20.591426783753157 29.55121221269837 30.456926085872176 19.400434917676296 78-79 19.32285559269825 29.219244972609694 31.255797469240598 20.202101965451458 80-81 19.323758163142525 31.264206876182765 29.838432676635634 19.573602284039072 82-83 19.053286282780242 31.549102269321395 29.414981940375895 19.98262950752247 84-85 19.08077963958406 29.648444593213924 29.708389942308074 21.562385824893948 86-87 19.457005655279538 30.70696463536026 29.399140222492044 20.436889486868157 88-89 20.648026603658003 28.752734751028264 30.261879758466787 20.33735888684694 90-91 18.891110683281994 28.75879480537665 31.68361453374405 20.666479977597312 92-93 18.927013463196076 30.020039466372637 30.57593776389308 20.477009306538204 94-95 18.547226196570538 29.948282425212973 30.053292729349067 21.45119864886742 96-97 18.872621943749234 29.87271820361587 28.955186656632304 22.29947319600259 98-99 19.163496268811816 29.529439304474142 29.337355855372977 21.969708571341062 100-101 18.76398815969966 30.70383278310504 29.371033269668743 21.161145787526554 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 805.0 1 542.0 2 238.5 3 187.5 4 152.5 5 147.5 6 184.5 7 261.5 8 329.5 9 383.0 10 376.5 11 311.5 12 333.0 13 394.0 14 431.5 15 449.0 16 479.5 17 518.5 18 562.5 19 618.5 20 710.5 21 855.0 22 992.5 23 1151.0 24 1385.5 25 1700.5 26 2180.5 27 2801.5 28 3186.5 29 3614.5 30 4329.0 31 4987.5 32 6104.5 33 7376.5 34 8451.5 35 9519.5 36 10667.0 37 11786.0 38 11709.0 39 10760.5 40 9797.0 41 8540.5 42 7364.0 43 7182.0 44 7405.0 45 7001.5 46 6564.5 47 7698.0 48 16629.5 49 16123.5 50 6455.0 51 4068.0 52 2771.5 53 2161.0 54 1707.0 55 1358.5 56 1054.0 57 806.0 58 624.0 59 480.5 60 377.0 61 286.0 62 189.5 63 112.5 64 68.0 65 52.5 66 35.5 67 25.5 68 19.0 69 11.0 70 6.5 71 2.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024502404298421786 2 0.006563144008505834 3 8.75085867800778E-4 4 0.0 5 0.005250515206804668 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00634437254155564 14-15 0.003281572004252917 16-17 0.0 18-19 8.75085867800778E-4 20-21 2.187714669501945E-4 22-23 8.75085867800778E-4 24-25 0.0 26-27 0.0 28-29 2.187714669501945E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.75085867800778E-4 38-39 0.012032430682260696 40-41 0.0 42-43 0.0 44-45 8.75085867800778E-4 46-47 0.0 48-49 0.0 50-51 0.0024064861364521397 52-53 6.563144008505835E-4 54-55 0.0 56-57 2.187714669501945E-4 58-59 0.0 60-61 0.002625257603402334 62-63 0.0 64-65 2.187714669501945E-4 66-67 0.0 68-69 2.187714669501945E-4 70-71 6.563144008505835E-4 72-73 4.37542933900389E-4 74-75 0.003500343471203112 76-77 0.0 78-79 4.37542933900389E-4 80-81 0.002844029070352528 82-83 2.187714669501945E-4 84-85 0.003281572004252917 86-87 6.563144008505835E-4 88-89 0.003937886405103501 90-91 0.0021877146695019447 92-93 0.0 94-95 0.0 96-97 4.37542933900389E-4 98-99 0.0010938573347509723 100-101 0.003281572004252917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 228549.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.71305063607872 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61577478354126 36.04577219963198 2 10.395758742604029 9.296521741170242 3 3.141497874890584 4.213978606593492 4 1.3905283627753098 2.486990603827043 5 0.8047187579352697 1.7990715285681045 6 0.5298145203639917 1.4213774086058952 7 0.3578747015587416 1.1201168756517303 8 0.300894650721017 1.0763134203051243 9 0.2629079506003793 1.0579874857040201 >10 1.8643472542447106 16.63592574150749 >50 0.22392160050981152 6.84674791123906 >100 0.09296745021166282 7.279865728577526 >500 0.011995800027311331 4.705295421178586 >1k 0.00699755001593161 6.014035327439714 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3102 1.3572581809590065 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2914 1.2750001093857335 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1887 0.8256435162700341 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1792 0.7840769375494971 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1604 0.7018188659762239 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1096 0.47954705555482635 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1060 0.4637955099344123 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 990 0.43316750456138503 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 979 0.4283545322884808 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 955 0.41785350187487147 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 953 0.4169784160070707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 941 0.411727900800266 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 940 0.41129035786636564 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 917 0.40122687038665666 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 904 0.3955388122459516 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 841 0.36797360741022717 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 778 0.3404084025745026 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 685 0.29971690972176646 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 644 0.2817776494318505 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 453 0.19820694905687622 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 443 0.19383151971787232 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 437 0.19120626211446998 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 419 0.183330489304263 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 341 0.14920214046003263 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 339 0.14832705459223186 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 333 0.1457017969888295 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 313 0.13695093831082175 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 292 0.12776253669891358 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 287 0.12557482202941164 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 278 0.12163693562430813 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 272 0.11901167802090579 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 269 0.11769904921920464 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 254 0.11113590521069878 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 253 0.11069836227679841 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 242 0.10588539000389414 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 241 0.10544784706999373 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 229 0.10019733186318908 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.1877146695019448E-4 0.0 16-17 0.0 0.0 0.0 6.563144008505835E-4 0.0 18-19 0.0 0.0 0.0 8.750858678007779E-4 0.0 20-21 0.0 0.0 0.0 0.0010938573347509723 0.0 22-23 0.0 0.0 0.0 0.0019689432025517505 0.0 24-25 0.0 0.0 0.0 0.005906829607655251 0.0 26-27 0.0 0.0 0.0 0.010938573347509724 0.0 28-29 0.0 0.0 0.0 0.026033804567073146 0.0 30-31 0.0 0.0 0.0 0.055349181138399206 0.0 32-33 0.0 0.0 0.0 0.09057138731738051 0.0 34-35 0.0 0.0 0.0 0.12098062122345755 0.0 36-37 0.0 0.0 0.0 0.1389198815133735 0.0 38-39 0.0 0.0 0.0 0.15357756979903653 0.0 40-41 0.0 0.0 0.0 0.17348577329150422 0.0 42-43 0.0 0.0 0.0 0.20105097812722872 0.0 44-45 0.0 0.0 0.0 0.22774109709515244 0.0 46-47 0.0 0.0 0.0 0.26318207474108396 0.0 48-49 0.0 0.0 0.0 0.30474865346162094 0.0 50-51 0.0 0.0 0.0 0.3454401463143571 0.0 52-53 0.0 0.0 0.0 0.4272606749537298 0.0 54-55 0.0 0.0 0.0 0.5276767782838692 0.0 56-57 0.0 0.0 0.0 0.6508451141768286 0.0 58-59 0.0 0.0 0.0 0.8269561450717352 0.0 60-61 0.0 0.0 0.0 1.0999829358255777 0.0 62-63 0.0 0.0 0.0 1.252247876822913 0.0 64-65 0.0 0.0 0.0 1.3856984716625318 0.0 66-67 0.0 0.0 0.0 1.703135870207264 0.0 68-69 0.0 0.0 0.0 2.0973620536515147 0.0 70-71 0.0 0.0 0.0 2.4007980783114347 0.0 72-73 0.0 0.0 0.0 2.6698869826601737 0.0 74-75 0.0 0.0 0.0 3.283322175988519 0.0 76-77 0.0 0.0 0.0 3.6593903276759034 0.0 78-79 0.0 0.0 0.0 3.8908505397092092 0.0 80-81 0.0 0.0 0.0 4.105246577320399 0.0 82-83 0.0 0.0 0.0 4.512161505847761 0.0 84-85 0.0 0.0 0.0 5.076373119112313 0.0 86-87 0.0 0.0 0.0 5.877951774017825 0.0 88-89 0.0 0.0 0.0 7.035909148585205 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 95 7.2759576E-12 50.00426 9 TAGGCAT 110 0.0 47.50405 5 GGGGTCC 40 5.38261E-4 47.493652 6 GGCATTC 100 1.2732926E-11 47.493652 7 TTAGGCA 145 0.0 39.313694 4 CTGGTCG 140 4.5110937E-10 33.931465 9 CCTTTAG 130 8.201823E-9 32.89462 1 GATGACC 60 0.0039427406 31.669365 9 GTATAGT 60 0.0039427406 31.669365 3 AGGCATT 150 9.476935E-10 31.662437 6 GCATTCC 155 1.3442332E-9 30.647772 8 GAGATCA 65 0.005831974 29.233263 9 CCGGACT 60 5.373384E-6 27.707664 64-65 TATCACC 155 4.5498382E-8 27.582996 3 ACTATAC 70 0.008374267 27.145172 3 ACGCAGC 70 0.008374267 27.145172 8 ACTACTC 70 0.008374267 27.145172 8 TCCCGCT 70 0.008374267 27.145172 8 GGGATTC 70 0.0083832145 27.13923 7 ACCCTGG 195 5.311449E-10 26.791292 6 >>END_MODULE