FastQCFastQC Report
Thu 26 May 2016
SRR936943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319773
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18880.5904188283563653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.4368724063632639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.27675882579204625No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.1028854843904895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10150.039.808691
GTATCAA17650.039.0524521
GTATTAC651.3200972E-436.5663531
TCGGACT600.00393501931.6858774
CAACGCA21850.031.3233385
TCAACGC21900.031.2518224
ATCAACG21750.031.2488293
TATCAAC22150.030.9039332
AACGCAG22150.030.8942556
GTACATG14700.028.4570791
TACATGG14700.027.486952
ACATGGG14150.027.2073083
ACGCAGA25300.027.0477377
CGCAGAG25400.026.7541588
GCAGAGT26600.025.3685519
GAGTACT14100.024.27003112-13
CATGGGA9100.023.503264
AGAGTAC22200.022.15523510-11
CATGGGG5800.022.1254834
AGTACTT14850.021.44397712-13
Produced by FastQC (version 0.11.5)