##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936943_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 319773 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92287654054595 33.0 31.0 34.0 30.0 34.0 2 32.025030255837734 33.0 31.0 34.0 30.0 34.0 3 32.02248470008412 34.0 31.0 34.0 30.0 34.0 4 35.579823812517006 37.0 35.0 37.0 33.0 37.0 5 35.60763416548614 37.0 35.0 37.0 33.0 37.0 6 35.676833253589265 37.0 35.0 37.0 33.0 37.0 7 35.61487367601392 37.0 35.0 37.0 33.0 37.0 8 35.57907953454482 37.0 35.0 37.0 33.0 37.0 9 37.239232205345665 39.0 37.0 39.0 34.0 39.0 10-11 37.12496208247725 39.0 37.0 39.0 34.0 39.0 12-13 37.04180621878645 39.0 37.0 39.0 33.0 39.0 14-15 38.23635829166315 40.0 38.0 41.0 33.5 41.0 16-17 38.16505771281503 40.0 38.0 41.0 33.0 41.0 18-19 38.131521423009445 40.0 38.0 41.0 33.5 41.0 20-21 38.08190184912422 40.0 38.0 41.0 33.0 41.0 22-23 37.983544576934264 40.0 38.0 41.0 33.0 41.0 24-25 37.817786367204235 40.0 38.0 41.0 33.0 41.0 26-27 37.759945336222884 40.0 37.5 41.0 33.0 41.0 28-29 37.81476703786748 40.0 38.0 41.0 33.0 41.0 30-31 37.65187179655568 40.0 37.0 41.0 33.0 41.0 32-33 37.44749556716796 40.0 37.0 41.0 32.5 41.0 34-35 37.23117492721399 40.0 36.0 41.0 31.5 41.0 36-37 37.06909119906934 40.0 36.0 41.0 31.0 41.0 38-39 36.893274291450496 39.0 36.0 41.0 31.0 41.0 40-41 36.67200482842516 39.0 36.0 41.0 30.5 41.0 42-43 36.467819359358046 39.0 35.5 41.0 30.0 41.0 44-45 36.170841503191326 39.0 35.0 41.0 30.0 41.0 46-47 35.77314063413734 39.0 35.0 40.0 28.5 41.0 48-49 35.38991878613892 39.0 35.0 40.0 26.5 41.0 50-51 34.75071222398389 38.5 34.5 39.5 26.0 40.5 52-53 35.06895985589777 38.5 35.0 40.0 26.5 40.5 54-55 35.38784231314088 39.0 35.0 40.5 27.0 41.0 56-57 35.26842166161621 39.0 35.0 41.0 26.0 41.0 58-59 35.042287810415516 39.0 35.0 41.0 26.0 41.0 60-61 34.746251246978325 38.0 34.5 40.0 25.0 41.0 62-63 34.49586269009579 38.0 34.0 40.0 24.5 41.0 64-65 34.19061177772983 38.0 34.0 40.0 23.5 41.0 66-67 33.80577159422465 37.0 34.0 40.0 22.5 41.0 68-69 33.377954048653265 36.5 33.0 39.5 22.0 41.0 70-71 33.01559387440466 36.0 33.0 39.0 20.5 41.0 72-73 32.644378981339884 36.0 33.0 39.0 19.5 40.5 74-75 32.219408768094866 35.0 32.0 38.0 18.0 40.0 76-77 31.773135943309786 35.0 32.0 37.0 15.0 39.5 78-79 31.331421039299755 35.0 31.5 37.0 11.0 39.0 80-81 30.89584330134189 35.0 31.0 36.0 7.5 39.0 82-83 30.468607418387418 35.0 31.0 36.0 6.0 37.5 84-85 30.095400487220623 34.0 31.0 35.5 2.0 37.0 86-87 29.76443914902134 34.0 30.0 35.0 2.0 37.0 88-89 29.3856016611784 34.0 30.0 35.0 2.0 36.0 90-91 29.03506862680714 34.0 29.5 35.0 2.0 36.0 92-93 28.662715426255502 34.0 29.0 35.0 2.0 35.5 94-95 28.26892358016468 34.0 29.0 35.0 2.0 35.0 96-97 27.828615924421385 34.0 29.0 35.0 2.0 35.0 98-99 27.252156998871072 34.0 27.0 35.0 2.0 35.0 100-101 25.24364001963893 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3168990774725806 1101 2 0.34146769601252913 1101 3 0.3227127180208953 1101 4 0.1783768251646265 1101 5 0.29531938299923866 1101 6 0.2615530486943669 1101 7 0.1654341975044744 1101 8 0.1496077526868831 1101 9 0.2729819501681092 1101 10-11 0.08405549933716117 1101 12-13 0.2313994753438422 1101 14-15 0.5127365932764505 1101 16-17 0.5370218821391859 1101 18-19 0.5898498787451061 1101 20-21 0.6341138384625111 1101 22-23 0.5170630655100581 1101 24-25 0.5984675233546497 1101 26-27 0.6358375682860142 1101 28-29 0.7784933482380936 1101 30-31 0.5293092787668812 1101 32-33 0.5326806867715277 1101 34-35 0.47348915720385776 1101 36-37 0.48470641650078505 1101 38-39 0.5810330281038389 1101 40-41 0.8637766035582786 1101 42-43 0.7626965045024932 1101 44-45 0.8158670140294149 1101 46-47 1.0398255015219107 1101 48-49 0.9321405372460774 1101 50-51 0.812522497757989 1101 52-53 0.6914938820999197 1101 54-55 0.7502510022410007 1101 56-57 0.8728521908013036 1101 58-59 0.8227090807031274 1101 60-61 1.024356135719536 1101 62-63 1.0790305633614068 1101 64-65 0.931914937042464 1101 66-67 0.8150423472465889 1101 68-69 0.9245065616963686 1101 70-71 0.8132780927561285 1101 72-73 0.8332819792155775 1101 74-75 1.035399124083753 1101 76-77 0.9215263056459264 1101 78-79 1.0300148130625004 1101 80-81 1.1468091512573686 1101 82-83 0.8786448256292765 1101 84-85 1.149996028778606 1101 86-87 1.1730863439359176 1101 88-89 1.2119822529558952 1101 90-91 1.063499943511811 1101 92-93 1.009761650257989 1101 94-95 1.1821451545619368 1101 96-97 1.3260963440868885 1101 98-99 1.109154557930605 1101 100-101 1.2011916468970654 1102 1 0.27813572988730684 1102 2 0.2668555109647315 1102 3 0.11984756946436192 1102 4 0.02241968261858318 1102 5 0.2011331592316452 1102 6 0.09192710773950807 1102 7 0.26074194509264714 1102 8 -0.014269017614878976 1102 9 -0.0943043977293101 1102 10-11 0.10890142591907903 1102 12-13 0.04214686752874286 1102 14-15 0.2976834932975052 1102 16-17 0.12662219863393176 1102 18-19 0.14213139937170638 1102 20-21 0.3155339271681541 1102 22-23 0.1271901715430559 1102 24-25 0.35728001473452764 1102 26-27 0.348796431227818 1102 28-29 0.4720832407315072 1102 30-31 0.4785739366113759 1102 32-33 0.4133745071389825 1102 34-35 0.5851733302887396 1102 36-37 0.512156528653172 1102 38-39 0.5156053609459761 1102 40-41 0.6733208075667321 1102 42-43 0.6161017266658391 1102 44-45 0.6410508495682876 1102 46-47 0.6858014450174394 1102 48-49 0.7418353666220199 1102 50-51 0.7191964718721948 1102 52-53 0.49782595477756075 1102 54-55 0.8768960853208654 1102 56-57 1.154635958105068 1102 58-59 1.031319770385835 1102 60-61 1.1072200502369185 1102 62-63 0.930664576384153 1102 64-65 1.1406798816195831 1102 66-67 0.9461590075010875 1102 68-69 0.9097198689938963 1102 70-71 1.006176560369859 1102 72-73 1.137630515427368 1102 74-75 1.2279546014028426 1102 76-77 1.059725987699366 1102 78-79 1.2326531161860714 1102 80-81 1.039220899310127 1102 82-83 1.1879168151181645 1102 84-85 1.3562021568718698 1102 86-87 1.3803486643650729 1102 88-89 1.123581495110674 1102 90-91 1.1029891964825786 1102 92-93 1.203794345036549 1102 94-95 1.2271603622797613 1102 96-97 1.2046523730940706 1102 98-99 0.9277126920633982 1102 100-101 1.0924582792042088 1103 1 0.2608296017004541 1103 2 0.267046295087475 1103 3 0.2182971974353194 1103 4 0.3008239240508672 1103 5 0.11970348326341451 1103 6 0.12394871655173034 1103 7 0.25100191356306567 1103 8 0.12938640617690567 1103 9 0.34201889098731897 1103 10-11 0.27854114516518536 1103 12-13 0.06249550040988794 1103 14-15 0.26666852176450817 1103 16-17 0.3391155462928097 1103 18-19 0.6606223973734942 1103 20-21 0.3593615587338874 1103 22-23 0.14588199493720566 1103 24-25 0.43149001784731666 1103 26-27 0.28957553333749075 1103 28-29 0.295171131964473 1103 30-31 0.40411290175813264 1103 32-33 0.41411505077331867 1103 34-35 0.5029654559251071 1103 36-37 0.688732254887995 1103 38-39 0.663794417970351 1103 40-41 0.885748886973083 1103 42-43 0.4907088619920259 1103 44-45 0.8482549584558186 1103 46-47 1.0990172620253347 1103 48-49 0.9799490137115541 1103 50-51 1.1745605181022043 1103 52-53 1.2232148029435237 1103 54-55 1.543912522879232 1103 56-57 1.6286491523333453 1103 58-59 1.4444979326326717 1103 60-61 1.429785795449746 1103 62-63 1.544143474656046 1103 64-65 1.7065531206319235 1103 66-67 1.6385422024322622 1103 68-69 1.646324815323318 1103 70-71 1.6788135985969816 1103 72-73 1.537252963689724 1103 74-75 1.5310327526042755 1103 76-77 1.4147375853657707 1103 78-79 1.4996805991410156 1103 80-81 1.5705622121057203 1103 82-83 1.5140045356495513 1103 84-85 1.6312425729820816 1103 86-87 1.805922773955487 1103 88-89 1.689560167454001 1103 90-91 1.7329985348633237 1103 92-93 1.7040980934425 1103 94-95 1.782718707076878 1103 96-97 1.495224122986123 1103 98-99 1.712420340498472 1103 100-101 1.8462890235635285 1104 1 0.27682580062348805 1104 2 0.3725085834765949 1104 3 0.30069785910427527 1104 4 0.33415373786849045 1104 5 0.363113190087752 1104 6 0.14633283649682483 1104 7 0.1734933787374544 1104 8 0.12610445777300328 1104 9 0.2615451641721265 1104 10-11 0.2523823446161444 1104 12-13 0.412489341257384 1104 14-15 0.536316218241474 1104 16-17 0.6142360897060328 1104 18-19 0.4355009740813429 1104 20-21 0.4052604430474034 1104 22-23 0.5344981213582116 1104 24-25 0.12958156045105795 1104 26-27 0.6359838675506992 1104 28-29 0.6786663630206746 1104 30-31 0.7541903311038567 1104 32-33 0.6415241592799887 1104 34-35 0.779916147509951 1104 36-37 0.7319299108905284 1104 38-39 0.8696464927248613 1104 40-41 1.0837216459214503 1104 42-43 1.1188016364262623 1104 44-45 1.2937376600360793 1104 46-47 1.441954473864989 1104 48-49 1.1465452900639264 1104 50-51 1.6032859254861407 1104 52-53 1.6400442472119963 1104 54-55 1.6875967519293198 1104 56-57 1.7400506234909088 1104 58-59 1.5344963488505954 1104 60-61 1.8993815790699173 1104 62-63 1.8177814220744821 1104 64-65 1.8658639994550015 1104 66-67 1.8659513109389323 1104 68-69 1.8710150979404219 1104 70-71 1.961835454789572 1104 72-73 2.0137294005320143 1104 74-75 1.8548721922973783 1104 76-77 1.8728245525990843 1104 78-79 1.7379474136273387 1104 80-81 1.6058453571449611 1104 82-83 1.239367925647084 1104 84-85 1.7610467119325612 1104 86-87 1.8762514967232349 1104 88-89 1.4250289669471918 1104 90-91 1.6957576592078105 1104 92-93 1.3373161626361743 1104 94-95 1.5053456736062856 1104 96-97 1.2603316441912042 1104 98-99 1.483159086846971 1104 100-101 1.4875183904026485 1105 1 0.2918357128296627 1105 2 0.239307412892245 1105 3 0.2711264440188401 1105 4 0.12311814832822421 1105 5 0.5019312775039708 1105 6 0.4105639325036208 1105 7 0.3680794261272382 1105 8 0.188999765995419 1105 9 0.5122295784079469 1105 10-11 0.562138787550154 1105 12-13 0.5790026047332617 1105 14-15 0.6136786273795778 1105 16-17 0.8215664021765363 1105 18-19 0.4698042875902644 1105 20-21 0.37765966895130276 1105 22-23 0.2810052487064638 1105 24-25 0.542106340966491 1105 26-27 0.47432050495276457 1105 28-29 0.32711345072010545 1105 30-31 0.2147303103354261 1105 32-33 0.1152450126737321 1105 34-35 -0.0733123996438394 1105 36-37 0.4081901771040677 1105 38-39 0.43651848366227597 1105 40-41 0.5575959025059873 1105 42-43 0.36655958979169867 1105 44-45 0.5136706346419473 1105 46-47 0.5888085284293183 1105 48-49 0.8658646519066551 1105 50-51 0.6702051174053381 1105 52-53 0.8825274908634668 1105 54-55 1.0068169917103091 1105 56-57 1.050279076242731 1105 58-59 1.2789384332459548 1105 60-61 1.075807048719689 1105 62-63 1.306276182765508 1105 64-65 1.0514818603065024 1105 66-67 1.1348435038965405 1105 68-69 1.3049455236932843 1105 70-71 1.2073041605386479 1105 72-73 1.1580346020802068 1105 74-75 1.1354348280002 1105 76-77 0.5874828174909936 1105 78-79 0.8958700984191843 1105 80-81 0.9751694390858638 1105 82-83 1.0400853805701473 1105 84-85 1.060997150901695 1105 86-87 0.9019288308117872 1105 88-89 0.8696268145824249 1105 90-91 0.6762046525052732 1105 92-93 0.7825984245072242 1105 94-95 0.9237115756170482 1105 96-97 0.972500057294198 1105 98-99 1.159903163123758 1105 100-101 1.5369850193085313 1106 1 0.2270126001253523 1106 2 0.5013310547648189 1106 3 0.40361054763339865 1106 4 0.30578788758482744 1106 5 0.3873279403299037 1106 6 0.36820913371558817 1106 7 0.5698888402014077 1106 8 0.3952687029646498 1106 9 0.7677026292337032 1106 10-11 0.7314192894065101 1106 12-13 0.6191276420737708 1106 14-15 1.147807846856388 1106 16-17 0.7581408911450822 1106 18-19 0.6058517420348437 1106 20-21 0.784031025585108 1106 22-23 1.0922330044355633 1106 24-25 0.7567608879728525 1106 26-27 1.0470811416616783 1106 28-29 0.3545000422790139 1106 30-31 0.5483303615452542 1106 32-33 1.0710765323255131 1106 34-35 0.7825105850358938 1106 36-37 0.40954510391667753 1106 38-39 0.7147585724259855 1106 40-41 0.6182795537670316 1106 42-43 0.37177659145601893 1106 44-45 0.6510090036087988 1106 46-47 1.0501771111972147 1106 48-49 1.2436118460345966 1106 50-51 1.0092462132957465 1106 52-53 0.5278780648403298 1106 54-55 0.12640261881738013 1106 56-57 0.7739166813295739 1106 58-59 1.5097454148530858 1106 60-61 1.5519518018456253 1106 62-63 1.4891699650383714 1106 64-65 1.4408259477046172 1106 66-67 1.3229009442584285 1106 68-69 0.8810988117912686 1106 70-71 1.0359325905305425 1106 72-73 0.8076099872208218 1106 74-75 1.1199545224481966 1106 76-77 1.1286533889073738 1106 78-79 1.0996638934945082 1106 80-81 0.9129921677164283 1106 82-83 1.4261328350147338 1106 84-85 1.30406248611272 1106 86-87 0.9320146072808697 1106 88-89 1.2324698137716013 1106 90-91 0.5730061717302988 1106 92-93 0.8974898169879104 1106 94-95 1.1673423527262514 1106 96-97 1.1634380507222701 1106 98-99 0.7019912440352947 1106 100-101 0.6582669721101375 1107 1 0.07609012011901939 1107 2 0.2747097862558334 1107 3 0.37585696461020035 1107 4 0.2595055216017741 1107 5 -0.04813805064325294 1107 6 0.3020518766089282 1107 7 0.22544351594770973 1107 8 0.41645011083542016 1107 9 0.18345209474957613 1107 10-11 0.6173762139452705 1107 12-13 0.5891767354303283 1107 14-15 0.38474267938469353 1107 16-17 0.7347621596540748 1107 18-19 0.2972762412034271 1107 20-21 0.41757287371243734 1107 22-23 0.36711620975665227 1107 24-25 0.7339562320222583 1107 26-27 0.547556238660647 1107 28-29 0.2377251590736975 1107 30-31 0.7625397209561342 1107 32-33 0.6835557468318072 1107 34-35 0.1876495509080911 1107 36-37 0.12466506590892124 1107 38-39 0.31033225205224113 1107 40-41 0.29299647513848726 1107 42-43 0.5167405632669286 1107 44-45 0.6744669179329676 1107 46-47 1.0323015866109486 1107 48-49 0.6492457046140601 1107 50-51 0.4389912445729678 1107 52-53 0.3213296445378546 1107 54-55 0.8387907730655328 1107 56-57 0.74695492663799 1107 58-59 0.9520607208947354 1107 60-61 1.3741071585642786 1107 62-63 1.2620142124055391 1107 64-65 1.1328839095384993 1107 66-67 0.9961331875080859 1107 68-69 1.300767827548647 1107 70-71 1.3862968315630795 1107 72-73 0.9367967795242507 1107 74-75 0.9301334756511466 1107 76-77 1.0224494139291451 1107 78-79 0.706046029847343 1107 80-81 1.1184716197712241 1107 82-83 1.0409677388075913 1107 84-85 0.6320179853604841 1107 86-87 1.0578361291749232 1107 88-89 0.7226511424595436 1107 90-91 0.6012942388773403 1107 92-93 1.2143597195930305 1107 94-95 1.463525740167853 1107 96-97 1.7056227050892048 1107 98-99 1.5423784480894547 1107 100-101 1.2579977504773865 1108 1 0.025916709714394415 1108 2 0.2042762602442707 1108 3 -0.054403150996733984 1108 4 -0.05428060556585734 1108 5 0.10897177594633689 1108 6 -0.3362429210789344 1108 7 -0.08100157075749337 1108 8 -0.17808746141891163 1108 9 -0.813050795423834 1108 10-11 -0.38087523114143096 1108 12-13 -0.22518742641938871 1108 14-15 -0.049794892869634566 1108 16-17 0.26769568566562896 1108 18-19 0.4770161255964922 1108 20-21 0.29755842284538403 1108 22-23 0.42778094964103985 1108 24-25 0.4823088331783296 1108 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-2.979889035966483 2316 82-83 -3.2955716338826413 2316 84-85 -2.8613204018279923 2316 86-87 -2.827855143448975 2316 88-89 -3.2012377990736702 2316 90-91 -3.301539078662696 2316 92-93 -3.0444482023249115 2316 94-95 -3.2150964525735475 2316 96-97 -3.1384595464032614 2316 98-99 -3.323688073278877 2316 100-101 -3.09731353541288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 980.0 3 219.0 4 229.0 5 236.0 6 291.0 7 336.0 8 361.0 9 451.0 10 575.0 11 813.0 12 967.0 13 1327.0 14 1537.0 15 1928.0 16 2544.0 17 3694.0 18 3159.0 19 1815.0 20 1751.0 21 1832.0 22 1921.0 23 2059.0 24 2315.0 25 2591.0 26 2987.0 27 3606.0 28 4190.0 29 5066.0 30 6149.0 31 7632.0 32 10001.0 33 13332.0 34 18680.0 35 27890.0 36 42628.0 37 65219.0 38 64539.0 39 13920.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48832915567489 19.22035818336009 15.287413513082173 22.003899147882848 2 14.82288418917396 20.197087512364924 41.926799644391025 23.053228654070097 3 18.672032948805416 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30.317798697774517 21.51502970907163 48-49 21.24572377837323 27.10659152091927 29.98618782369844 21.66149687700906 50-51 21.17260481359781 27.327153552487733 29.854222024814003 21.64601960910045 52-53 21.29154160064693 27.4957025256167 29.52407915173565 21.68867672200072 54-55 21.229854257687176 27.24710181313636 29.599908647085233 21.92313528209123 56-57 21.295363533975724 27.51392191215117 29.348329370541858 21.84238518333125 58-59 21.46872061862605 27.142796801742115 29.572308881251143 21.816173698380688 60-61 21.11569989362368 27.02991051874101 30.15299418058945 21.70139540704587 62-63 21.11552102024821 27.034481248152037 30.098542435510943 21.75145529608881 64-65 20.968256600043173 27.19884125797339 29.99746603390509 21.835436108078348 66-67 20.925644607915995 27.222236997936438 29.65047787267263 22.201640521474935 68-69 21.00748054776006 27.09297341605424 29.920470295248553 21.979075740937148 70-71 21.022315850055755 27.061623900746156 29.844184726255886 22.071875522942207 72-73 21.071538042900308 26.99050413176612 29.629704927467333 22.308252897866247 74-75 20.91313031469401 27.436294052234732 29.489679894523313 22.160895738547946 76-77 21.10262085578645 27.11862285799201 29.549112694452482 22.229643591769058 78-79 21.069989752254894 27.17107476160303 29.43308848262968 22.325847003512393 80-81 20.8994001392115 27.244316182163725 29.400217420207564 22.456066258417213 82-83 21.100369094911684 27.29393504617989 29.37926653534252 22.226429323565906 84-85 20.980866746251657 26.95818971789051 29.155329977829897 22.90561355802794 86-87 21.006748056393914 26.944818641095086 29.293261174659353 22.75517212785165 88-89 20.97660901993249 26.841333506518705 29.204531405759248 22.977526067789555 90-91 20.8038590743166 26.733446301694368 29.477544800091376 22.985149823897657 92-93 20.83122085563171 26.88714674678434 29.496760867524173 22.784871530059775 94-95 20.61951334011423 26.996948595571553 29.408027540880994 22.975510523433222 96-97 20.468834862256085 27.09534937228343 29.462531871035196 22.97328389442529 98-99 20.72487752485122 27.021407513061668 28.942494218734588 23.31122074335252 100-101 20.54255374109403 27.088548540368247 29.06269520334024 23.306202515197487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 92.0 1 75.0 2 64.5 3 72.5 4 65.5 5 70.5 6 137.5 7 266.5 8 459.0 9 545.5 10 456.5 11 418.0 12 448.0 13 479.5 14 505.0 15 546.5 16 573.0 17 602.0 18 672.5 19 723.5 20 792.0 21 887.5 22 1005.5 23 1203.5 24 1503.0 25 1891.0 26 2348.5 27 2982.0 28 3495.0 29 3971.5 30 4640.0 31 5408.5 32 6097.0 33 6700.0 34 7398.5 35 7991.0 36 8755.0 37 9488.5 38 9915.5 39 10187.5 40 10399.0 41 10623.0 42 10719.0 43 11115.0 44 11633.5 45 11806.0 46 11892.0 47 12222.5 48 12813.0 49 12639.0 50 11617.0 51 10604.5 52 9937.5 53 9581.0 54 8948.5 55 8113.5 56 7323.5 57 6448.5 58 5746.0 59 5046.5 60 4191.5 61 3300.5 62 2588.0 63 1951.0 64 1315.0 65 973.0 66 742.5 67 528.0 68 341.5 69 210.0 70 149.5 71 92.5 72 60.0 73 55.5 74 43.0 75 32.0 76 24.0 77 16.5 78 11.5 79 5.5 80 2.5 81 2.5 82 3.5 83 3.5 84 3.0 85 3.0 86 2.0 87 0.5 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06754791680348246 2 0.10038370969406422 3 0.07724229375213042 4 0.13978666116276234 5 0.11633252338377537 6 0.1144561923614564 7 0.12133607277662593 8 0.10663814643512741 9 0.12727778768063597 10-11 0.11336166593177036 12-13 0.09694376948647947 14-15 0.09975826601995791 16-17 0.11211077858355771 18-19 0.09100205458246943 20-21 0.07395871446307224 22-23 0.0727078271148596 24-25 0.05941714904010032 26-27 0.05785353985483452 28-29 0.06848608231464196 30-31 0.049879133009978954 32-33 0.05394451689167002 34-35 0.04659555372092078 36-37 0.04831552382471316 38-39 0.04753371923208025 40-41 0.06019895363273322 42-43 0.05582084791398899 44-45 0.042373808920703124 46-47 0.027988604416257782 48-49 0.03987203422427785 50-51 0.02329777686046039 52-53 0.03393031932026781 54-55 0.0417483652465968 56-57 0.041592004328070226 58-59 0.05050457668408527 60-61 0.04784644106913342 62-63 0.05144274219524475 64-65 0.03627573309816651 66-67 0.05613356975104214 68-69 0.045031944535654976 70-71 0.02110872400108827 72-73 0.017199701037923776 74-75 0.02548682971983251 76-77 0.039246590550171524 78-79 0.059573509958626904 80-81 0.03611937217963993 82-83 0.06488978118853062 84-85 0.062075284655052174 86-87 0.06238800649210534 88-89 0.0842785350858265 90-91 0.06707883404790273 92-93 0.07599140640391779 94-95 0.07755501558918357 96-97 0.1005400706125908 98-99 0.1321249761549599 100-101 0.12336876471747146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 319773.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.98318302289545 #Duplication Level Percentage of deduplicated Percentage of total 1 89.56177191381656 71.63435594837605 2 7.005096733665001 11.205798682836296 3 1.5869513064068186 3.8078825036627877 4 0.6663460381265491 2.1318590849622816 5 0.33566103832369715 1.3423619130949695 6 0.19942334242883025 0.9570308217913609 7 0.1390077552980389 0.7782797913523437 8 0.10293537052790001 0.6586478864370068 9 0.06922994548942804 0.49835082606713976 >10 0.3132685955725636 4.191956974342785 >50 0.014449894860689939 0.7765381035272425 >100 0.004686452387140909 0.7143775714942266 >500 3.9053769892840906E-4 0.2764425670189727 >1k 7.810753978568181E-4 1.0261173250365259 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1888 0.5904188283563653 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1397 0.4368724063632639 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 885 0.27675882579204625 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 329 0.1028854843904895 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.127218370531596E-4 0.0 20-21 0.0 0.0 0.0 3.127218370531596E-4 0.0 22-23 0.0 0.0 0.0 9.381655111594788E-4 0.0 24-25 0.0 0.0 0.0 0.002345413777898697 0.0 26-27 0.0 0.0 0.0 0.0029708574520050162 0.0 28-29 0.0 0.0 0.0 0.006879880415169511 0.0 30-31 0.0 0.0 0.0 0.011414347052440325 0.0 32-33 0.0 0.0 0.0 0.017356061956450355 0.0 34-35 0.0 0.0 0.0 0.020796002164035113 0.0 36-37 0.0 0.0 0.0 0.027832243497731203 0.0 38-39 0.0 0.0 0.0 0.03611937217963993 0.0 40-41 0.0 0.0 0.0 0.04362469626891576 0.0 42-43 0.0 0.0 0.0 0.05972987087715348 0.0 44-45 0.0 0.0 0.0 0.08568578335256573 0.0 46-47 0.0 0.0 0.0 0.11039080847976533 1.563609185265798E-4 48-49 0.0 0.0 0.0 0.1759060333424023 3.127218370531596E-4 50-51 0.0 0.0 0.0 0.22734877553764704 3.127218370531596E-4 52-53 0.0 0.0 0.0 0.2911440302964916 3.127218370531596E-4 54-55 0.0 0.0 0.0 0.4131055467472238 3.127218370531596E-4 56-57 0.0 0.0 0.0 0.5392888079981737 3.127218370531596E-4 58-59 0.0 0.0 0.0 0.6851735449834726 3.127218370531596E-4 60-61 0.0 0.0 0.0 0.8504470358660675 3.127218370531596E-4 62-63 0.0 0.0 0.0 1.0863956619226762 3.127218370531596E-4 64-65 0.0 0.0 0.0 1.2568290631166483 3.127218370531596E-4 66-67 0.0 0.0 0.0 1.487305057024827 3.127218370531596E-4 68-69 0.0 0.0 0.0 1.7784490873213186 3.127218370531596E-4 70-71 0.0 0.0 0.0 1.9617040838344701 3.127218370531596E-4 72-73 0.0 0.0 0.0 2.2641061002648755 3.127218370531596E-4 74-75 0.0 0.0 0.0 2.6279579576762266 3.127218370531596E-4 76-77 0.0 0.0 0.0 2.9062803926535388 3.127218370531596E-4 78-79 0.0 0.0 0.0 3.1389454394210894 3.127218370531596E-4 80-81 0.0 0.0 0.0 3.4644888717934283 3.127218370531596E-4 82-83 0.0 0.0 0.0 3.8184899913376054 3.127218370531596E-4 84-85 0.0 0.0 0.0 4.308525109999906 3.127218370531596E-4 86-87 0.0 0.0 0.0 5.0770390245580455 3.127218370531596E-4 88-89 0.0 0.0 0.0 5.787699399261351 3.127218370531596E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1015 0.0 39.80869 1 GTATCAA 1765 0.0 39.052452 1 GTATTAC 65 1.3200972E-4 36.566353 1 TCGGACT 60 0.003935019 31.685877 4 CAACGCA 2185 0.0 31.323338 5 TCAACGC 2190 0.0 31.251822 4 ATCAACG 2175 0.0 31.248829 3 TATCAAC 2215 0.0 30.903933 2 AACGCAG 2215 0.0 30.894255 6 GTACATG 1470 0.0 28.457079 1 TACATGG 1470 0.0 27.48695 2 ACATGGG 1415 0.0 27.207308 3 ACGCAGA 2530 0.0 27.047737 7 CGCAGAG 2540 0.0 26.754158 8 GCAGAGT 2660 0.0 25.368551 9 GAGTACT 1410 0.0 24.270031 12-13 CATGGGA 910 0.0 23.50326 4 AGAGTAC 2220 0.0 22.155235 10-11 CATGGGG 580 0.0 22.125483 4 AGTACTT 1485 0.0 21.443977 12-13 >>END_MODULE