FastQCFastQC Report
Thu 26 May 2016
SRR936943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319773
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21550.6739155588495589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17340.5422596654501787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.3242925450241265No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5970.18669493672073628No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.1338449462587523No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCCG651.3260673E-436.5383387
GCGAGTG300.003949716731.66656390-91
GGTATCA17400.029.764961
GTATAGC804.4783406E-429.696691
GTATCAA24700.028.470351
GGTAAGG1205.3658932E-627.7082423
CTCCACG700.00837002327.1512581
TGTTCGC350.0083889727.14276728-29
GAGTACT18700.024.13093812-13
GTACATG15350.023.8347361
TAGACTC1000.001651077123.749925
GTAATAG1000.001651077123.749925
TATCAAC30000.023.4332562
GTACTTT19500.023.38453914-15
ATCAACG30250.023.2395943
TCAACGC30250.023.2395944
GTAAGGT1252.2510116E-422.7999254
CAACGCA30900.022.7507345
GGCGTGC1050.002192823222.6189738
AACGCAG31400.022.5397346
Produced by FastQC (version 0.11.5)