FastQCFastQC Report
Thu 26 May 2016
SRR936942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193809
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.6939822196079646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.4953330340696252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.29565190471030756No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.1238332585174063No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG608.2185936E-539.6213381
GTCTAGG500.001604911838.0364841
CTCTAGG651.3166512E-436.573541
GGTATCA8000.035.6592061
GTATCAA12750.035.053231
GTACTGG753.0518367E-431.6970731
ATTAGAT600.00392442731.6970713
TAGCACA804.448143E-429.7236844
ATCAACG15350.028.496393
TCAACGC15450.028.3192654
TATCAAC15450.028.3119492
CAACGCA15550.028.1371485
AACGCAG15750.027.772676
TAGGGGT700.00832501627.1759434
GTAATTG700.00833552227.168921
CTATTAG700.00833552227.168921
CCTACTC1056.831909E-527.1689173
TATATAG1109.375385E-525.933972
TTACACT950.001214216525.0304724
ACGCAGA17950.024.368787
Produced by FastQC (version 0.11.5)