##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936942_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 193809 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.994107600782215 33.0 31.0 34.0 30.0 34.0 2 32.1064192065384 33.0 31.0 34.0 30.0 34.0 3 32.13098978891589 34.0 31.0 34.0 30.0 34.0 4 35.67775490302308 37.0 35.0 37.0 35.0 37.0 5 35.681841400554156 37.0 35.0 37.0 35.0 37.0 6 35.750852643582085 37.0 35.0 37.0 35.0 37.0 7 35.69270260926995 37.0 35.0 37.0 35.0 37.0 8 35.65213173794819 37.0 35.0 37.0 35.0 37.0 9 37.33746110861725 39.0 37.0 39.0 35.0 39.0 10-11 37.182107126088056 39.0 37.0 39.0 34.0 39.0 12-13 37.144559850161755 39.0 37.0 39.0 34.0 39.0 14-15 38.35791939486815 40.0 38.0 41.0 34.0 41.0 16-17 38.2954351965079 40.0 38.0 41.0 34.0 41.0 18-19 38.31288278666109 40.0 38.0 41.0 34.0 41.0 20-21 38.26956694477553 40.0 38.0 41.0 34.0 41.0 22-23 38.15902770253187 40.0 38.0 41.0 34.0 41.0 24-25 38.00766218287076 40.0 38.0 41.0 33.0 41.0 26-27 38.058449298020214 40.0 38.0 41.0 34.0 41.0 28-29 38.00536610786909 40.0 38.0 41.0 33.5 41.0 30-31 37.82853221470623 40.0 37.5 41.0 33.0 41.0 32-33 37.58933795644165 40.0 37.0 41.0 33.0 41.0 34-35 37.39505905298516 40.0 37.0 41.0 33.0 41.0 36-37 37.19308700834327 40.0 36.5 41.0 32.0 41.0 38-39 37.03516348569984 40.0 36.0 41.0 31.5 41.0 40-41 36.83709734842035 39.5 36.0 41.0 31.0 41.0 42-43 36.58014591685629 39.0 36.0 41.0 30.5 41.0 44-45 36.266517550784535 39.0 35.5 41.0 30.0 41.0 46-47 35.83237362557982 39.0 35.0 41.0 28.5 41.0 48-49 35.42871332084681 39.0 35.0 40.0 26.5 41.0 50-51 34.787631121361756 38.5 34.5 40.0 25.5 40.5 52-53 35.003454431940725 38.5 35.0 40.0 26.0 40.5 54-55 35.37846281648427 39.0 35.0 41.0 26.5 41.0 56-57 35.26353781300146 39.0 35.0 41.0 26.0 41.0 58-59 35.02738004943011 39.0 35.0 41.0 25.5 41.0 60-61 34.77877704337776 39.0 35.0 41.0 25.0 41.0 62-63 34.486685344849825 38.0 34.5 40.0 23.5 41.0 64-65 34.18591241892791 38.0 34.0 40.0 22.5 41.0 66-67 33.804227357862636 37.0 34.0 40.0 22.0 41.0 68-69 33.43479404981193 37.0 34.0 39.5 20.5 41.0 70-71 33.00483723666083 36.0 33.0 39.0 18.5 41.0 72-73 32.61492500348281 36.0 33.0 39.0 17.5 40.5 74-75 32.19547079856973 35.0 32.5 38.5 12.5 40.0 76-77 31.75731519176096 35.0 32.0 37.5 8.0 39.5 78-79 31.294106568838394 35.0 32.0 37.0 7.0 39.0 80-81 30.863801474647723 35.0 31.0 36.5 3.5 39.0 82-83 30.45445773932067 35.0 31.0 36.0 2.0 37.5 84-85 30.094139075068753 35.0 31.0 35.5 2.0 37.0 86-87 29.740569323406035 34.0 30.5 35.0 2.0 37.0 88-89 29.37469880139725 34.0 30.0 35.0 2.0 36.0 90-91 29.01623247630399 34.0 30.0 35.0 2.0 36.0 92-93 28.66098581593218 34.0 29.0 35.0 2.0 36.0 94-95 28.258883746368845 34.0 29.0 35.0 2.0 35.0 96-97 27.79101589709456 34.0 29.0 35.0 2.0 35.0 98-99 27.205638025065916 34.0 27.0 35.0 2.0 35.0 100-101 25.196195739103963 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.35546398954982905 1101 2 0.2601797505670618 1101 3 0.1847993370774219 1101 4 0.18616018950638846 1101 5 0.2541635077566866 1101 6 0.14403394039264583 1101 7 0.2053000696951912 1101 8 0.20603900291565935 1101 9 0.13824290986460852 1101 10-11 0.153562946871169 1101 12-13 0.14759317050763343 1101 14-15 0.3634676861510471 1101 16-17 0.4915078529838084 1101 18-19 0.45887544619182563 1101 20-21 0.3296543399877194 1101 22-23 0.5173461394472412 1101 24-25 0.6328376015312571 1101 26-27 0.5180695761145202 1101 28-29 0.5556837807820685 1101 30-31 0.46336223857614556 1101 32-33 0.545187910416665 1101 34-35 0.5477807936896824 1101 36-37 0.440234062421446 1101 38-39 0.5679248417194671 1101 40-41 0.834940229200761 1101 42-43 0.6890863873530577 1101 44-45 0.9726048477886096 1101 46-47 1.17475745471544 1101 48-49 1.204903753681485 1101 50-51 1.2586541171817487 1101 52-53 1.267719317113503 1101 54-55 1.3233448746733245 1101 56-57 1.4693924369603977 1101 58-59 1.2266756313535367 1101 60-61 1.5845820806367144 1101 62-63 1.475787822189048 1101 64-65 1.4514691485717748 1101 66-67 1.1384332764337088 1101 68-69 1.4275886085633473 1101 70-71 1.445092842171647 1101 72-73 1.5285260663058722 1101 74-75 1.4538815228986408 1101 76-77 1.2951856155428096 1101 78-79 1.2770520087638992 1101 80-81 1.3446314860991144 1101 82-83 1.4409286157823082 1101 84-85 1.7707432013865514 1101 86-87 1.668096657431704 1101 88-89 1.647229359774304 1101 90-91 1.5132672438431527 1101 92-93 1.5035830447053087 1101 94-95 1.598082056479111 1101 96-97 1.653442682349091 1101 98-99 1.7779160535176146 1101 100-101 1.4926213022786534 1102 1 0.16677351335935597 1102 2 -0.0314443990928055 1102 3 0.1438129425195953 1102 4 0.013371073860128035 1102 5 0.03128935809682076 1102 6 0.05598121930420774 1102 7 0.14811469554553014 1102 8 -0.2772518134108708 1102 9 0.08798780782379367 1102 10-11 0.28540818496640696 1102 12-13 0.06846902084777184 1102 14-15 0.03740476098096934 1102 16-17 0.15696278495659044 1102 18-19 0.07148173870883312 1102 20-21 0.14891454406935623 1102 22-23 0.1583325340050692 1102 24-25 0.14055443826595138 1102 26-27 0.23212144686282699 1102 28-29 0.28984619574806203 1102 30-31 0.11627465354213484 1102 32-33 0.3335807675595177 1102 34-35 0.4049449093359456 1102 36-37 -0.13312903281665456 1102 38-39 -0.029609171885972785 1102 40-41 0.06799720198987558 1102 42-43 -5.394629870778545E-4 1102 44-45 0.4302791675165025 1102 46-47 0.31504231866102117 1102 48-49 0.3144913387155057 1102 50-51 0.1841218042565771 1102 52-53 0.318399589222345 1102 54-55 0.3638423236529178 1102 56-57 0.5584655662120923 1102 58-59 0.7474026721698621 1102 60-61 0.4148329309768499 1102 62-63 0.6497248970189773 1102 64-65 0.6793815635377669 1102 66-67 0.4231058954813349 1102 68-69 0.32099847250892566 1102 70-71 0.11407668570906537 1102 72-73 0.372616202360291 1102 74-75 0.12086706711632189 1102 76-77 0.06973918697137904 1102 78-79 0.2508615325734205 1102 80-81 0.4459495133099942 1102 82-83 0.22898133687074207 1102 84-85 0.02516497009403551 1102 86-87 0.13873866423442394 1102 88-89 0.4990575910668227 1102 90-91 0.44991690370709847 1102 92-93 0.5717378066100736 1102 94-95 0.46812882518659293 1102 96-97 0.47030067554637256 1102 98-99 0.5158412235856424 1102 100-101 1.1226170505779685 1103 1 0.5707450852657097 1103 2 0.4362883210855699 1103 3 0.4169734776366525 1103 4 0.18271332581107913 1103 5 0.28200006043795867 1103 6 0.3239647756029811 1103 7 -0.017750689069856662 1103 8 0.28014088447095276 1103 9 0.3157051991281463 1103 10-11 0.2595511615550379 1103 12-13 0.14477197513986084 1103 14-15 0.2044825201782956 1103 16-17 0.43186175374143687 1103 18-19 0.16684475431640777 1103 20-21 0.45229386402476024 1103 22-23 0.7593916198467596 1103 24-25 0.49383969467620403 1103 26-27 0.11562548811142648 1103 28-29 0.3483572046666765 1103 30-31 0.2669052276781443 1103 32-33 0.473679708473675 1103 34-35 0.6452912025354962 1103 36-37 0.5954140273840238 1103 38-39 0.6176455661296316 1103 40-41 0.6070405408973443 1103 42-43 0.7829053530664325 1103 44-45 1.0239978130014507 1103 46-47 0.8684529931314771 1103 48-49 1.210167899473582 1103 50-51 1.2157759291172212 1103 52-53 1.2087842046069568 1103 54-55 1.229523065012998 1103 56-57 1.1583471158219112 1103 58-59 1.3743362005979591 1103 60-61 1.4577363590704948 1103 62-63 1.2603645291260008 1103 64-65 1.2389864966481312 1103 66-67 1.491455254455694 1103 68-69 1.566796967492202 1103 70-71 1.302625320045749 1103 72-73 1.3698082313458002 1103 74-75 0.9049912309959183 1103 76-77 1.2278182003493079 1103 78-79 0.9913576306782161 1103 80-81 0.7546660684939432 1103 82-83 1.2144537448975008 1103 84-85 0.9866755609524525 1103 86-87 0.7517229173001994 1103 88-89 0.5620104896175455 1103 90-91 0.6843789995822362 1103 92-93 0.7599136706011542 1103 94-95 0.7389029333248338 1103 96-97 0.8712378271628687 1103 98-99 1.5697988601519732 1103 100-101 1.92464812638622 1104 1 -0.09352441410178614 1104 2 -0.04997850962821104 1104 3 -0.026005710330146314 1104 4 -0.28835604185662334 1104 5 -0.26670286988245095 1104 6 0.21063363726621276 1104 7 0.08707194943153951 1104 8 -0.06007780650241301 1104 9 -0.23914949787568673 1104 10-11 -0.18347134871234516 1104 12-13 -0.5226687166306334 1104 14-15 -0.44410430637653775 1104 16-17 -0.015484322124578398 1104 18-19 -0.534352674071819 1104 20-21 -0.4611135215610389 1104 22-23 -0.022064702609782216 1104 24-25 -0.004366762079477837 1104 26-27 -0.19756875175548316 1104 28-29 -0.04788588542638195 1104 30-31 -0.05341986286546074 1104 32-33 0.21148448085831006 1104 34-35 -0.05607085059497763 1104 36-37 0.23165399827143318 1104 38-39 0.4036593946079776 1104 40-41 0.23115026762027213 1104 42-43 0.3194087235414216 1104 44-45 0.3161114214025105 1104 46-47 0.3080097193518654 1104 48-49 0.4401659932923607 1104 50-51 0.45075608750354945 1104 52-53 0.5156793819684538 1104 54-55 0.7102099834111186 1104 56-57 0.5624055489409443 1104 58-59 0.7777621281284084 1104 60-61 0.8033530086970586 1104 62-63 1.107982669039707 1104 64-65 0.7885298795999418 1104 66-67 0.7991475621480006 1104 68-69 1.2281552082602119 1104 70-71 1.5019598895087256 1104 72-73 1.589790209268756 1104 74-75 1.3714337769608846 1104 76-77 0.9624691092511704 1104 78-79 1.0964142096027807 1104 80-81 1.2258394096830543 1104 82-83 1.0966950674406881 1104 84-85 1.40137395109576 1104 86-87 1.1650609872050666 1104 88-89 1.8503637223276144 1104 90-91 1.8988590453305854 1104 92-93 1.486558618354458 1104 94-95 1.4797922535112917 1104 96-97 1.9148980416949009 1104 98-99 2.1985700664181103 1104 100-101 1.566944554895759 1105 1 0.30318655683761264 1105 2 0.5711824125014004 1105 3 0.4470738120848168 1105 4 0.47424063907752156 1105 5 0.7028473291113144 1105 6 0.3256370164056577 1105 7 0.337879188299155 1105 8 0.3866068822413027 1105 9 -0.21328030811823595 1105 10-11 0.5220929675751051 1105 12-13 0.284682788963714 1105 14-15 0.3619518624302458 1105 16-17 0.16597546611602354 1105 18-19 0.22370094160738319 1105 20-21 0.10335294986645494 1105 22-23 -0.23949355295145835 1105 24-25 0.328009148410878 1105 26-27 0.20843485265992712 1105 28-29 0.18726467400892943 1105 30-31 -0.35433223051583695 1105 32-33 -0.23535039186077 1105 34-35 -0.22399530805536472 1105 36-37 0.03159017008189835 1105 38-39 0.2999198136212726 1105 40-41 -0.1775191023579481 1105 42-43 0.18786633411437492 1105 44-45 0.05967228345853215 1105 46-47 0.23746079692189426 1105 48-49 0.3486851792952095 1105 50-51 0.19961093469135704 1105 52-53 0.013689444294811892 1105 54-55 -0.004590647361581546 1105 56-57 0.44207063867586527 1105 58-59 0.15750230985101865 1105 60-61 0.47529488749859183 1105 62-63 0.42173576658419876 1105 64-65 0.483412360639214 1105 66-67 0.005669301278437899 1105 68-69 0.3689151391755914 1105 70-71 0.7878538596221105 1105 72-73 1.0871995356936281 1105 74-75 0.9621261250873374 1105 76-77 0.5840054913192034 1105 78-79 0.3062963151821201 1105 80-81 0.5618009625853588 1105 82-83 0.4187458296243669 1105 84-85 0.4719041005288176 1105 86-87 0.08199047582862562 1105 88-89 0.27577860555957656 1105 90-91 0.3529966138520244 1105 92-93 0.388676212407173 1105 94-95 0.5872864338822446 1105 96-97 0.5602010378652089 1105 98-99 0.7412035424262235 1105 100-101 0.848839876664929 1106 1 0.48707167849697086 1106 2 0.2024814418857872 1106 3 0.4275285388498702 1106 4 0.5726982909855138 1106 5 0.2820921103904013 1106 6 0.3772923813837181 1106 7 0.2659472643528673 1106 8 0.49237739148209414 1106 9 0.8697538628696648 1106 10-11 0.8733095611131958 1106 12-13 0.6724636691658219 1106 14-15 0.3437694398800559 1106 16-17 0.6508179072807536 1106 18-19 0.7775501637852855 1106 20-21 1.0979015471274565 1106 22-23 0.8545099040356519 1106 24-25 0.8184691936176307 1106 26-27 0.6945736639882014 1106 28-29 0.8736095749529511 1106 30-31 1.2285357391690397 1106 32-33 1.1056946819326043 1106 34-35 0.7885362701922105 1106 36-37 1.093849178192528 1106 38-39 1.3563617761262563 1106 40-41 0.6400251071886558 1106 42-43 0.9054238397652199 1106 44-45 0.8711335253146686 1106 46-47 0.9650901015356439 1106 48-49 0.7944703142506668 1106 50-51 0.5096760856021376 1106 52-53 0.928261974543446 1106 54-55 1.3045437762523022 1106 56-57 1.7500748934292218 1106 58-59 1.4599886813441714 1106 60-61 1.2478317841878592 1106 62-63 1.5099886584868685 1106 64-65 1.4761992240882194 1106 66-67 1.9688723327902053 1106 68-69 1.9820649862703874 1106 70-71 2.1801221750057067 1106 72-73 2.3624288934917956 1106 74-75 2.438957663446601 1106 76-77 2.2473481319255093 1106 78-79 2.333353887313489 1106 80-81 1.627084834410912 1106 82-83 1.3720432634762467 1106 84-85 1.6894616061796626 1106 86-87 1.5749103003200524 1106 88-89 1.516737254675384 1106 90-91 1.930612622361533 1106 92-93 2.322062730157473 1106 94-95 2.259537464330325 1106 96-97 2.172087755057074 1106 98-99 2.3733335783257026 1106 100-101 1.3273666683149763 1107 1 0.47506790588272096 1107 2 0.14100497785423727 1107 3 0.2959157462579185 1107 4 0.5669536283772629 1107 5 0.5524342179099051 1107 6 0.42539321307368283 1107 7 0.42293946190066833 1107 8 0.7644343236607796 1107 9 0.8532915461415556 1107 10-11 0.9337142597327599 1107 12-13 0.43028755667332064 1107 14-15 0.5753136394856497 1107 16-17 0.6344842024020778 1107 18-19 0.6800195112944962 1107 20-21 0.9209745129167075 1107 22-23 0.8996097925720434 1107 24-25 0.9913235192628349 1107 26-27 1.1093897490435083 1107 28-29 0.5234423795486762 1107 30-31 0.24186850089416367 1107 32-33 -0.30950723867101004 1107 34-35 0.09806212117394608 1107 36-37 -0.02533113562039091 1107 38-39 -0.37886929649656764 1107 40-41 0.5266323266004704 1107 42-43 0.49712408841479316 1107 44-45 0.4744088404136946 1107 46-47 0.31195625947098904 1107 48-49 0.22857817672173297 1107 50-51 0.16229548026903728 1107 52-53 0.03265192567094033 1107 54-55 -0.147012427125901 1107 56-57 -0.0035476736632915618 1107 58-59 0.07874028899229302 1107 60-61 -0.25967057369604873 1107 62-63 -0.06820734597167899 1107 64-65 0.36276357288355854 1107 66-67 0.41129709485827703 1107 68-69 -0.11083563964061227 1107 70-71 -0.3571752613314345 1107 72-73 -0.3240154175773995 1107 74-75 0.09239159982660183 1107 76-77 0.26848334584988365 1107 78-79 -0.2088736699187912 1107 80-81 -0.4969827296806564 1107 82-83 -0.11067987808252866 1107 84-85 0.15032073333390983 1107 86-87 0.24604980130607856 1107 88-89 0.3852530739328621 1107 90-91 0.06771285386286152 1107 92-93 0.32467783776272086 1107 94-95 0.43132002456354 1107 96-97 0.7355362674466797 1107 98-99 0.8871807874485711 1107 100-101 0.602124449331864 1108 1 0.1177792604857899 1108 2 -0.42232083587441593 1108 3 -0.20360085058385025 1108 4 -0.05674386866860459 1108 5 -0.026038228110074613 1108 6 -0.3052975163279754 1108 7 -0.06991691364986963 1108 8 -0.19485238812351469 1108 9 -0.7207765599922951 1108 10-11 -0.2175372173324419 1108 12-13 0.011069595560414314 1108 14-15 0.3038559104062628 1108 16-17 -0.1793518702158181 1108 18-19 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-1.2659002483964628 2316 92-93 -1.1503697985679224 2316 94-95 -1.6250042654282915 2316 96-97 -2.3267765898257977 2316 98-99 -1.6417639544078853 2316 100-101 -1.505669761719762 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 597.0 3 158.0 4 144.0 5 157.0 6 155.0 7 196.0 8 226.0 9 281.0 10 345.0 11 423.0 12 679.0 13 828.0 14 938.0 15 1225.0 16 1741.0 17 2366.0 18 2068.0 19 1295.0 20 1098.0 21 1127.0 22 1129.0 23 1212.0 24 1391.0 25 1522.0 26 1659.0 27 1961.0 28 2314.0 29 2718.0 30 3469.0 31 4171.0 32 5663.0 33 7635.0 34 10636.0 35 15832.0 36 25167.0 37 39889.0 38 42012.0 39 9372.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.413201470041706 19.398046826609406 15.280588016682495 21.90816368666639 2 14.620776512609812 20.27015866789169 42.423272667210355 22.685792152288144 3 18.735871842776188 25.833754812605154 30.997822070374998 24.43255127424366 4 12.024839462327847 17.05777291404266 39.49484834093865 31.42253928269084 5 12.843297723482364 37.34852044425397 35.273373159913874 14.534808672349786 6 29.597158111052597 37.05660026642709 18.868304470398712 14.477937152121607 7 26.433292028087568 33.402519619991736 22.542854192482444 17.62133415943825 8 25.09578148396757 34.21645066349977 21.387411576392836 19.300356276139826 9 25.731516525277144 16.580008571148277 22.00255069111337 35.68592421246121 10-11 23.62460466016911 26.91047569870264 28.801394178015876 20.66352546311237 12-13 25.086557453235734 24.602197178007565 29.38151117307618 20.92973419568052 14-15 22.22692666152047 25.42333499422989 28.44935083040525 23.90038751384439 16-17 20.53030733251075 28.919612388293174 28.88140878373146 21.66867149546461 18-19 20.33822185743267 26.958635962399146 32.138303418869604 20.56483876129858 20-21 21.421749567641516 26.00965385508892 32.121783123822304 20.446813453447252 22-23 20.991130253911646 26.051814594693635 32.11379701004137 20.843258141353353 24-25 20.739347400905157 26.228074249531687 32.25530114202261 20.77727720754055 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27.43644122458036 30.324256825441054 21.530519048282482 52-53 20.927514544821268 27.46139656084315 30.058179285078495 21.552909609257085 54-55 20.84698621725243 27.09850200987652 30.24283642856037 21.811675344310682 56-57 20.993050914373743 27.52591392216921 29.797720452193747 21.6833147112633 58-59 21.046344219744153 27.177497174762244 30.181279639195207 21.5948789662984 60-61 20.9464951552763 26.899247809875238 30.570142051685657 21.58411498316281 62-63 20.69495798102502 26.91949437897839 30.72248898888697 21.663058651109626 64-65 20.65774094419316 27.017898748351904 30.608227180192433 21.7161331272625 66-67 20.54785330371127 27.16430696505474 30.238498873690812 22.049340857543175 68-69 20.59086123611742 26.814541100697742 30.68385904793361 21.91073861525123 70-71 20.687146008850586 26.827078145763718 30.476718832651695 22.009057012734004 72-73 20.668433397929984 26.740174385149135 30.293678215790653 22.297714001130224 74-75 20.615534677046856 27.0069903724328 30.111758224460882 22.26571672605946 76-77 20.64270728768028 26.964236407693082 30.054423418843562 22.338632885783074 78-79 20.534645084031876 26.649409505719124 30.16398604286245 22.651959367386546 80-81 20.492188266140897 26.973837014250545 29.922269764176995 22.61170495543157 82-83 20.538541540319645 27.233664781891708 29.868822759754423 22.358970918034228 84-85 20.42054905158422 26.807486175656255 29.57983800423697 23.192126768522556 86-87 20.67438577839251 26.678932605290523 29.631531547804908 23.015150068512064 88-89 20.51012772545478 26.439169139465875 29.788414398142177 23.26228873693717 90-91 20.204769879011344 26.399987614725063 30.18755950737569 23.207682998887904 92-93 20.423483870967743 26.525419354838707 30.012903225806454 23.0381935483871 94-95 20.05542710870732 26.856085628173897 30.122878906733824 22.965608356384955 96-97 20.01383325762894 27.05692282281042 29.7897654540199 23.139478465540737 98-99 20.342500761514355 27.066801588080107 28.98323635856742 23.607461291838113 100-101 20.332511520439905 26.879735643014808 29.14587393992591 23.64187889661938 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 55.0 1 44.0 2 41.5 3 48.5 4 39.5 5 47.0 6 88.0 7 156.5 8 277.5 9 364.0 10 322.5 11 280.5 12 289.5 13 316.5 14 359.0 15 375.0 16 380.0 17 411.0 18 432.5 19 463.0 20 520.0 21 589.5 22 698.5 23 802.0 24 976.5 25 1219.0 26 1502.5 27 1910.0 28 2303.5 29 2598.5 30 3034.5 31 3486.5 32 3866.5 33 4209.0 34 4551.5 35 4941.5 36 5461.0 37 5972.5 38 6174.0 39 6231.0 40 6300.0 41 6443.5 42 6610.5 43 6836.0 44 7027.0 45 7102.5 46 7145.0 47 7300.0 48 7675.5 49 7536.5 50 6933.5 51 6373.0 52 5861.5 53 5611.0 54 5299.0 55 4795.0 56 4205.0 57 3634.0 58 3247.0 59 2805.5 60 2316.5 61 1871.5 62 1425.5 63 1077.0 64 782.5 65 541.0 66 373.0 67 275.0 68 205.0 69 134.5 70 80.0 71 46.5 72 28.0 73 21.0 74 16.5 75 16.5 76 12.5 77 7.5 78 6.0 79 3.0 80 0.5 81 2.0 82 3.0 83 1.5 84 1.5 85 2.0 86 1.0 87 0.0 88 1.5 89 1.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037665949465711085 2 0.0366340056447327 3 0.024250679792992072 4 0.0438576123915814 5 0.07017217982653025 6 0.06965620791604105 7 0.06656037645310589 8 0.07172009555799781 9 0.07017217982653025 10-11 0.07558988488666676 12-13 0.07816974443911273 14-15 0.07146210960275323 16-17 0.05675691015381123 18-19 0.046437471944027364 20-21 0.053661078690876066 22-23 0.031216300584596176 24-25 0.015479157314675787 26-27 0.014189227538452807 28-29 0.016511101135654176 30-31 0.010835410120273052 32-33 0.0064496488811149125 34-35 0.010577424165028456 36-37 0.006965620791604105 38-39 0.007739578657337894 40-41 0.008513536523071684 42-43 0.003869789328668947 44-45 0.0012899297762229824 46-47 0.003869789328668947 48-49 0.001031943820978386 50-51 0.006191662925870315 52-53 0.005933676970625719 54-55 0.008255550567827088 56-57 0.0219288061957907 58-59 0.010319438209783859 60-61 0.021412834285301507 62-63 0.014189227538452807 64-65 0.01264131180698523 66-67 0.01780103091187716 68-69 0.021154848330056913 70-71 0.018832974732855542 72-73 0.0219288061957907 74-75 0.0219288061957907 76-77 0.026056581479704244 78-79 0.037665949465711085 80-81 0.03199025845032997 82-83 0.03276421631606375 84-85 0.01986491855383393 86-87 0.024508665748236666 88-89 0.017543044956632562 90-91 0.015737143269920387 92-93 0.030442342718862384 94-95 0.021154848330056913 96-97 0.037665949465711085 98-99 0.059852741616746384 100-101 0.06785030622932887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 193809.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.15382366673748 #Duplication Level Percentage of deduplicated Percentage of total 1 86.71301113037624 66.03527359234518 2 9.017240333136744 13.733946605805777 3 2.0435468934305305 4.668717293310534 4 0.797784325924355 2.430173075221215 5 0.42962602331299726 1.6358832211009822 6 0.26277969463045103 1.2007007116851895 7 0.16256270339169943 0.8665840014215739 8 0.11711363029372314 0.7134920600287742 9 0.07181077330132105 0.4921798470624777 >10 0.36092269276523314 4.566904998502743 >50 0.013767716338065544 0.7017221393125956 >100 0.006883858169032772 0.7990795332407038 >500 0.001966816619723649 1.148068345321461 >1k 9.834083098618244E-4 1.0072745756408124 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1345 0.6939822196079646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 960 0.4953330340696252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 573 0.29565190471030756 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 240 0.1238332585174063 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.15971910489193E-4 0.0 16-17 0.0 0.0 0.0 5.15971910489193E-4 0.0 18-19 0.0 0.0 0.0 5.15971910489193E-4 0.0 20-21 0.0 0.0 0.0 7.739578657337895E-4 0.0 22-23 0.0 0.0 0.0 0.002063887641956772 0.0 24-25 0.0 0.0 0.0 0.0038697893286689473 0.0 26-27 0.0 0.0 0.0 0.007223606746848701 0.0 28-29 0.0 0.0 0.0 0.01109339607551765 0.0 30-31 0.0 0.0 0.0 0.01831700282236635 0.0 32-33 0.0 0.0 0.0 0.027346511255927228 0.0 34-35 0.0 0.0 0.0 0.03508608991326512 0.0 36-37 0.0 0.0 0.0 0.044115598346826 0.0 38-39 0.0 0.0 0.0 0.05211316295940849 0.0 40-41 0.0 0.0 0.0 0.062432601169192345 0.0 42-43 0.0 0.0 0.0 0.0871992528726736 0.0 44-45 0.0 0.0 0.0 0.1243492304278955 0.0 46-47 0.0 0.0 0.0 0.1679488568642323 0.0 48-49 0.0 0.0 0.0 0.25953387097606406 0.0 50-51 0.0 0.0 0.0 0.33460778395224167 0.0 52-53 0.0 0.0 0.0 0.4236129385116274 0.0 54-55 0.0 0.0 0.0 0.5987854021227085 0.0 56-57 0.0 0.0 0.0 0.7837613320330841 0.0 58-59 0.0 0.0 0.0 1.0249782001867818 0.0 60-61 0.0 0.0 0.0 1.296637411059342 0.0 62-63 0.0 0.0 0.0 1.7060611220325166 0.0 64-65 0.0 0.0 0.0 1.9635311053666238 0.0 66-67 0.0 0.0 0.0 2.3035565943790024 0.0 68-69 0.0 0.0 0.0 2.7555479879675353 0.0 70-71 0.0 0.0 0.0 3.0579075275142023 0.0 72-73 0.0 0.0 0.0 3.515058640207627 0.0 74-75 0.0 0.0 0.0 4.089077390626854 0.0 76-77 0.0 0.0 0.0 4.527911500497913 0.0 78-79 0.0 0.0 0.0 4.872322750749449 0.0 80-81 0.0 0.0 0.0 5.315026649949177 0.0 82-83 0.0 0.0 0.0 5.7817232429866525 0.0 84-85 0.0 0.0 0.0 6.453776656398826 0.0 86-87 0.0 0.0 0.0 7.55073293809885 0.0 88-89 0.0 0.0 0.0 8.498315351712254 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 60 8.2185936E-5 39.621338 1 GTCTAGG 50 0.0016049118 38.036484 1 CTCTAGG 65 1.3166512E-4 36.57354 1 GGTATCA 800 0.0 35.659206 1 GTATCAA 1275 0.0 35.05323 1 GTACTGG 75 3.0518367E-4 31.697073 1 ATTAGAT 60 0.003924427 31.697071 3 TAGCACA 80 4.448143E-4 29.723684 4 ATCAACG 1535 0.0 28.49639 3 TCAACGC 1545 0.0 28.319265 4 TATCAAC 1545 0.0 28.311949 2 CAACGCA 1555 0.0 28.137148 5 AACGCAG 1575 0.0 27.77267 6 TAGGGGT 70 0.008325016 27.175943 4 GTAATTG 70 0.008335522 27.16892 1 CTATTAG 70 0.008335522 27.16892 1 CCTACTC 105 6.831909E-5 27.168917 3 TATATAG 110 9.375385E-5 25.93397 2 TTACACT 95 0.0012142165 25.030472 4 ACGCAGA 1795 0.0 24.36878 7 >>END_MODULE