FastQCFastQC Report
Thu 26 May 2016
SRR936942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193809
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.6614759892471453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.5732447925534934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.35602061823754316No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3460.17852628102926077No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.1398283877425713No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.1171256236810468No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGC250.005000390657.0419921
GGTATCA8700.038.2465481
GTATCAA14350.037.431741
CCTAATA550.002571578834.553062
TAAGGTG1308.236384E-932.8748785
ATCAACG18200.029.4906353
CCCTAAG650.005811282429.2523021
TAGTCAG650.005840691729.2221155
TATCAAC18400.029.1776182
TCAACGC18650.028.7716434
CAACGCA18700.028.6947145
GTAAGGT1004.9421396E-528.4915644
AACGCAG19150.028.0204266
GAGTTAG700.00834461427.1628551
TATACTG908.936056E-426.3810775
TTAACCC1109.455842E-525.901424
GTACATG10950.025.1783521
TACATGG11000.025.0509662
GTAGAGC950.001223147424.999053
CGCAGAG21700.024.2900438
Produced by FastQC (version 0.11.5)