FastQCFastQC Report
Thu 26 May 2016
SRR936941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204874
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.6501557054579888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.505676659800658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.29091051085057157No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.12251432587834472No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAA405.357249E-447.5367851
GTATCAA13850.037.4116251
GGTATCA8150.034.996411
ACTATCC600.00392851231.6911938
ATCAACG16500.030.8268853
TATCAAC16700.030.4577032
CAACGCA16700.030.4577035
TCAACGC16700.030.4577034
TACCTGG1252.1248161E-730.4235462
AACGCAG17000.029.9202126
ATATAAC1153.8210783E-628.9354363
GAGCTAG856.3601916E-427.9628161
AGGGGGT700.00834416727.1638777
CGCCGTG350.00840369127.13071858-59
ACGCAGA19050.026.7004557
CGCAGAG19350.026.2864918
CTAGACT1109.389792E-525.9291554
GCAGAGT19650.025.6432539
TACATGG8450.025.315452
ATACACT950.00121760225.0193614
Produced by FastQC (version 0.11.5)