##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936941_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 204874 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97099680779406 33.0 31.0 34.0 30.0 34.0 2 32.09790895867704 33.0 31.0 34.0 30.0 34.0 3 32.101037710983334 34.0 31.0 34.0 30.0 34.0 4 35.645645616330036 37.0 35.0 37.0 35.0 37.0 5 35.62917695754464 37.0 35.0 37.0 33.0 37.0 6 35.73877602819294 37.0 35.0 37.0 35.0 37.0 7 35.6754493005457 37.0 35.0 37.0 33.0 37.0 8 35.64486952956451 37.0 35.0 37.0 33.0 37.0 9 37.3103273231352 39.0 37.0 39.0 34.0 39.0 10-11 37.19562999697375 39.0 37.0 39.0 34.0 39.0 12-13 37.11279615763836 39.0 37.0 39.0 34.0 39.0 14-15 38.327232835791754 40.0 38.0 41.0 34.0 41.0 16-17 38.253494830969274 40.0 38.0 41.0 33.5 41.0 18-19 38.24253687632398 40.0 38.0 41.0 33.5 41.0 20-21 38.17865614963343 40.0 38.0 41.0 34.0 41.0 22-23 38.019599851616114 40.0 38.0 41.0 33.0 41.0 24-25 38.13670597537999 40.0 38.0 41.0 34.0 41.0 26-27 38.091146265509536 40.0 38.0 41.0 34.0 41.0 28-29 37.99787186270586 40.0 38.0 41.0 33.0 41.0 30-31 37.772682233958434 40.0 37.5 41.0 33.0 41.0 32-33 37.53024297861124 40.0 37.0 41.0 33.0 41.0 34-35 37.3258148911038 40.0 37.0 41.0 32.5 41.0 36-37 37.11441910637758 40.0 36.0 41.0 31.5 41.0 38-39 36.96034147817683 39.5 36.0 41.0 31.0 41.0 40-41 36.74503353280553 39.0 36.0 41.0 31.0 41.0 42-43 36.48307252262366 39.0 35.5 41.0 30.0 41.0 44-45 36.157858000527156 39.0 35.0 41.0 30.0 41.0 46-47 35.758493024981206 39.0 35.0 40.5 28.5 41.0 48-49 35.452258461298165 39.0 35.0 40.5 27.0 41.0 50-51 34.75109335494011 38.0 34.0 40.0 25.5 40.5 52-53 34.92996915177133 38.5 35.0 40.0 26.0 40.5 54-55 35.313922215605686 39.0 35.0 40.5 26.0 41.0 56-57 35.21140798734832 39.0 35.0 41.0 26.0 41.0 58-59 34.97020607788202 39.0 35.0 41.0 25.0 41.0 60-61 34.72537022755449 38.5 35.0 40.5 24.5 41.0 62-63 34.40066577506174 38.0 34.0 40.0 23.0 41.0 64-65 34.06296308950867 38.0 34.0 40.0 22.0 41.0 66-67 33.695532375996954 37.0 34.0 40.0 21.5 41.0 68-69 33.312091822290775 37.0 33.5 39.5 20.0 41.0 70-71 32.9098397063561 36.0 33.0 39.0 18.5 41.0 72-73 32.52544978865058 36.0 33.0 39.0 16.5 40.5 74-75 32.090628874332516 35.0 32.0 38.5 12.5 40.0 76-77 31.647839647783513 35.0 32.0 37.0 9.0 39.5 78-79 31.215717953473842 35.0 31.5 37.0 7.0 39.0 80-81 30.75710680711071 35.0 31.0 36.0 3.5 39.0 82-83 30.327227954742916 35.0 31.0 36.0 2.0 37.5 84-85 29.922174116774215 34.0 30.5 35.5 2.0 37.0 86-87 29.542938586643498 34.0 30.0 35.0 2.0 37.0 88-89 29.179710456182825 34.0 29.5 35.0 2.0 36.0 90-91 28.763698663568828 34.0 29.0 35.0 2.0 36.0 92-93 28.406764645587046 34.0 29.0 35.0 2.0 36.0 94-95 27.990638148325313 34.0 29.0 35.0 2.0 35.0 96-97 27.479328758163554 34.0 27.5 35.0 2.0 35.0 98-99 26.85096449525074 34.0 26.5 35.0 2.0 35.0 100-101 24.478218319552504 31.5 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22931849655657643 1101 2 0.20107030047122976 1101 3 0.35773425912160306 1101 4 0.260840898799934 1101 5 0.290557943112816 1101 6 0.12263904251262403 1101 7 0.09017051941495424 1101 8 0.0566579460994987 1101 9 0.16574086284188638 1101 10-11 0.04384950626905493 1101 12-13 0.23404405010456486 1101 14-15 -0.14133979834176102 1101 16-17 0.23050049406987938 1101 18-19 0.2192328507499255 1101 20-21 0.5285789210182443 1101 22-23 0.3979452932824401 1101 24-25 0.38792696677918315 1101 26-27 0.3429227334617764 1101 28-29 0.11647393095986303 1101 30-31 0.33909283831112447 1101 32-33 0.49576607969124353 1101 34-35 0.4338810137221216 1101 36-37 0.5084566504602677 1101 38-39 0.39852800005783706 1101 40-41 0.5043653790699878 1101 42-43 0.3273068518663109 1101 44-45 0.48639745615412266 1101 46-47 0.7281854494917042 1101 48-49 0.8290423121728736 1101 50-51 0.9275194077402418 1101 52-53 1.0052279479799822 1101 54-55 1.16797915937682 1101 56-57 1.0061328584399476 1101 58-59 0.913013562258989 1101 60-61 1.167675229271076 1101 62-63 1.3507751007163193 1101 64-65 1.2951188195826475 1101 66-67 1.509237655142151 1101 68-69 1.2829846209035694 1101 70-71 1.3417005013683792 1101 72-73 1.1370479802228637 1101 74-75 1.0510396353927653 1101 76-77 1.4741249173683677 1101 78-79 1.5296424192646292 1101 80-81 1.3145742588521863 1101 82-83 1.2476723173045663 1101 84-85 1.3875578513007518 1101 86-87 1.3434691743594591 1101 88-89 1.2440596580558783 1101 90-91 1.16276950071666 1101 92-93 1.3255599089846584 1101 94-95 1.458438532092316 1101 96-97 1.3712879040830508 1101 98-99 1.2517080853919644 1101 100-101 1.2551936058204198 1102 1 0.14480724733944683 1102 2 0.1577788807092233 1102 3 -0.10062677571002254 1102 4 0.0019354693062680894 1102 5 -0.10232638506563063 1102 6 -0.01443278867685649 1102 7 0.07335691436605174 1102 8 -0.06542617010227048 1102 9 0.10188288721920458 1102 10-11 0.11157605634854662 1102 12-13 -0.237895536540897 1102 14-15 0.2807443178599982 1102 16-17 0.06671960087722795 1102 18-19 0.11709863096864837 1102 20-21 0.15704160830446057 1102 22-23 0.22065088657626575 1102 24-25 -0.2835814400233403 1102 26-27 0.016883325069429134 1102 28-29 -0.019892114229712377 1102 30-31 0.26660644817767576 1102 32-33 0.3380891514260824 1102 34-35 0.2376508263154662 1102 36-37 0.4121349276679567 1102 38-39 0.41791423120878335 1102 40-41 0.2142512007205042 1102 42-43 0.558732395056559 1102 44-45 0.3434167749381487 1102 46-47 0.4674541299368542 1102 48-49 0.15620905110431949 1102 50-51 0.6457204965295631 1102 52-53 0.7943834136060346 1102 54-55 0.8776795014301015 1102 56-57 1.026123704901373 1102 58-59 0.8089137405121107 1102 60-61 0.9863797626814872 1102 62-63 0.9433318285671675 1102 64-65 1.0365723051537756 1102 66-67 0.9466539484407974 1102 68-69 1.1037944681839988 1102 70-71 1.1481344754494174 1102 72-73 1.1623791937893806 1102 74-75 1.2352305107601076 1102 76-77 1.1682497909050014 1102 78-79 1.1706767691225082 1102 80-81 1.1010270217755398 1102 82-83 0.9617037283948022 1102 84-85 0.9809913917931148 1102 86-87 1.076670022266189 1102 88-89 1.183465641579506 1102 90-91 1.2993402961109872 1102 92-93 1.1647177834366502 1102 94-95 1.0161347309649855 1102 96-97 0.6781361962254628 1102 98-99 0.4682446754579672 1102 100-101 -0.2462589162203379 1103 1 0.2668102929887688 1103 2 0.2979141642025098 1103 3 0.1764741911065677 1103 4 0.44667109986192344 1103 5 0.3102882694656586 1103 6 0.15410494550170029 1103 7 0.28851787339144863 1103 8 0.22291890904919853 1103 9 0.06312168737503043 1103 10-11 0.12297068042334303 1103 12-13 0.42474567888566384 1103 14-15 0.5619356625495939 1103 16-17 0.753079695788152 1103 18-19 0.5466148685292964 1103 20-21 0.6866392992772958 1103 22-23 0.7977394583083921 1103 24-25 0.39438541197934995 1103 26-27 0.645765642596082 1103 28-29 0.5072531207456663 1103 30-31 0.6764056478093678 1103 32-33 0.7275869734326719 1103 34-35 0.2803430386609662 1103 36-37 0.5247265627566762 1103 38-39 0.7105887362907595 1103 40-41 0.6320009616724533 1103 42-43 0.48822207172192833 1103 44-45 0.5959046454626389 1103 46-47 0.727290514821938 1103 48-49 0.8534591130797367 1103 50-51 0.659895160976923 1103 52-53 1.0964516219851106 1103 54-55 1.1571355342947882 1103 56-57 1.1895706008180795 1103 58-59 1.0913800081751077 1103 60-61 0.9298493238954961 1103 62-63 0.8028423998034881 1103 64-65 0.9684259014989962 1103 66-67 1.0089303942388597 1103 68-69 0.901497836105527 1103 70-71 1.0029349145895523 1103 72-73 0.6335517687796361 1103 74-75 0.9961204778246007 1103 76-77 0.8276151625804644 1103 78-79 1.1382636232500722 1103 80-81 1.3484594685652134 1103 82-83 1.5181633381267154 1103 84-85 1.4751391411999215 1103 86-87 1.5249559591575021 1103 88-89 1.2962343493034183 1103 90-91 1.1733088480109721 1103 92-93 1.114083863402655 1103 94-95 1.318264914766381 1103 96-97 0.9294288264623844 1103 98-99 0.908199344863359 1103 100-101 0.8304755102413992 1104 1 0.10148929749450275 1104 2 0.018619356376447627 1104 3 0.028374762638815554 1104 4 -0.10549255794447987 1104 5 0.12094175157881892 1104 6 -0.05887397735656208 1104 7 -0.08235817366168163 1104 8 -0.18811559456445792 1104 9 0.01123018778326923 1104 10-11 -0.059791120659241415 1104 12-13 -0.046987666543586215 1104 14-15 -0.1650594083627368 1104 16-17 0.24803720886686875 1104 18-19 0.338633072889877 1104 20-21 0.30353881241649106 1104 22-23 0.35402328908646297 1104 24-25 0.267416044898404 1104 26-27 0.3102928432976384 1104 28-29 -0.047564079653497515 1104 30-31 -0.36760838345859526 1104 32-33 -0.12031438433442787 1104 34-35 0.2707192217932075 1104 36-37 0.2278321903837579 1104 38-39 0.12438262180287296 1104 40-41 0.6652193837596059 1104 42-43 0.09459057394455073 1104 44-45 0.4401923469353193 1104 46-47 -0.07941667317891188 1104 48-49 0.20223742509401887 1104 50-51 -0.1250166900013383 1104 52-53 0.23989412100231533 1104 54-55 0.4627465294866582 1104 56-57 0.588176545963087 1104 58-59 0.8554793156836524 1104 60-61 0.9945171396746204 1104 62-63 1.155514086277222 1104 64-65 1.080494606328294 1104 66-67 1.3066336813052999 1104 68-69 0.6091847936790842 1104 70-71 0.48585698662075316 1104 72-73 1.0740095993762715 1104 74-75 1.249484655755598 1104 76-77 1.761216118158199 1104 78-79 1.1587057291498546 1104 80-81 1.092199829628445 1104 82-83 1.2499060578944743 1104 84-85 1.351904389619893 1104 86-87 1.3058534764697889 1104 88-89 1.208085327561001 1104 90-91 1.2586228883508674 1104 92-93 0.672665919104368 1104 94-95 1.0585125787417056 1104 96-97 0.5519000230513349 1104 98-99 1.0178564255129068 1104 100-101 0.6736065393018471 1105 1 0.09078633382059209 1105 2 0.08856671408229744 1105 3 0.2047991584157991 1105 4 -0.02059595206366538 1105 5 0.18591572127314748 1105 6 0.21088142502252083 1105 7 0.0011133093386348492 1105 8 0.27714339970597024 1105 9 0.4980986983844389 1105 10-11 0.34443480494352485 1105 12-13 0.29797932295704044 1105 14-15 0.4063276142938008 1105 16-17 0.6335474901105087 1105 18-19 0.44781493601990974 1105 20-21 0.3545960209206882 1105 22-23 0.2747399223063667 1105 24-25 0.3402136483652285 1105 26-27 0.12681554346924173 1105 28-29 -0.12552411804455943 1105 30-31 -0.38212136952729026 1105 32-33 0.26518135483900096 1105 34-35 -0.09012770837576767 1105 36-37 0.12419085600338775 1105 38-39 0.36188371245813755 1105 40-41 -0.15474978705154996 1105 42-43 0.09882231637541139 1105 44-45 0.2559734015262478 1105 46-47 -0.25210375691215603 1105 48-49 -0.13606929625083808 1105 50-51 0.15840825837431538 1105 52-53 -0.19546637691816926 1105 54-55 0.33361294602971725 1105 56-57 0.3129096989747424 1105 58-59 0.4355188701146133 1105 60-61 0.24176141736999313 1105 62-63 0.5175680082735568 1105 64-65 0.8898626079570064 1105 66-67 0.3808564076526224 1105 68-69 0.7933019445966849 1105 70-71 0.6140307934438951 1105 72-73 0.4152829030437779 1105 74-75 0.8299459302226246 1105 76-77 1.1351509115153782 1105 78-79 0.8594008401058524 1105 80-81 0.6207313713787279 1105 82-83 0.33471541135402916 1105 84-85 0.7435514244917769 1105 86-87 0.6108588570357689 1105 88-89 0.3468312194261678 1105 90-91 0.5478501227399555 1105 92-93 0.7082649069762823 1105 94-95 1.1969821712729 1105 96-97 0.5666456822433759 1105 98-99 0.5836011249283182 1105 100-101 0.9736821580669357 1106 1 -0.08914997191189045 1106 2 0.3048377042386363 1106 3 0.40249265387999245 1106 4 -0.037725849766815145 1106 5 -0.050187347632473234 1106 6 0.17005918993277191 1106 7 0.2984014776459105 1106 8 0.2857324751740222 1106 9 0.301080748181775 1106 10-11 0.41794860536816003 1106 12-13 0.48269269875321896 1106 14-15 0.2856462786443217 1106 16-17 0.40562334419272617 1106 18-19 0.8022590587761371 1106 20-21 0.4110523034916227 1106 22-23 0.2868273571645048 1106 24-25 0.26476971862194176 1106 26-27 0.06657138211042479 1106 28-29 -0.03526644578052185 1106 30-31 -0.1656573664390848 1106 32-33 -0.09551156158639884 1106 34-35 -0.33030817546706714 1106 36-37 -0.3385335215299037 1106 38-39 -0.14491999339015393 1106 40-41 -0.5266133269087234 1106 42-43 -0.579859405299942 1106 44-45 0.007855277611945155 1106 46-47 0.17556464687090312 1106 48-49 0.6350414931720536 1106 50-51 0.7611393913602242 1106 52-53 0.4995973173493482 1106 54-55 0.40753689893455913 1106 56-57 0.57224669955378 1106 58-59 0.10771053195596636 1106 60-61 0.2582157698116063 1106 62-63 0.33058673052795484 1106 64-65 0.5907166901805212 1106 66-67 0.0705398664443635 1106 68-69 0.39991746283640595 1106 70-71 0.8114588961267728 1106 72-73 0.9373346305095147 1106 74-75 0.6516743610274922 1106 76-77 0.15889150213494574 1106 78-79 0.6216571612793693 1106 80-81 1.0448772891552167 1106 82-83 0.9262759209081715 1106 84-85 1.1198060085489097 1106 86-87 0.46971815060843625 1106 88-89 0.43079184478806454 1106 90-91 0.761684324631485 1106 92-93 1.1580332914580396 1106 94-95 1.144699793353574 1106 96-97 1.4593304921256411 1106 98-99 1.7895459232298023 1106 100-101 1.5777161283429457 1107 1 0.5591551128338743 1107 2 0.6222491063957989 1107 3 0.2806385193139995 1107 4 0.20713804340216058 1107 5 0.07423484034903538 1107 6 0.3487957076523358 1107 7 0.6395673738965542 1107 8 0.6206066405566659 1107 9 0.7105568652850067 1107 10-11 0.7237395658201322 1107 12-13 0.48269269875321896 1107 14-15 0.7345420156756433 1107 16-17 0.7178344381834805 1107 18-19 0.5736381034602616 1107 20-21 0.4692187133529515 1107 22-23 0.8929521645604908 1107 24-25 0.704998275375921 1107 26-27 0.42320980018951815 1107 28-29 0.6791018130792636 1107 30-31 0.7424063212856211 1107 32-33 0.6823518179718988 1107 34-35 0.4881946493916942 1107 36-37 -0.12262443062081019 1107 38-39 -0.045921534222202354 1107 40-41 0.4210625847502385 1107 42-43 0.6449736712280512 1107 44-45 0.18196929918359217 1107 46-47 -0.5696844543103978 1107 48-49 -0.4678732474545555 1107 50-51 0.025648893157857344 1107 52-53 0.005953249552739237 1107 54-55 0.14007413570909222 1107 56-57 0.05818660710386325 1107 58-59 0.6132960481346927 1107 60-61 0.3570858263087828 1107 62-63 0.4624562734144462 1107 64-65 0.630200511443995 1107 66-67 0.7078023215034221 1107 68-69 0.4729143811723091 1107 70-71 0.04059346212574866 1107 72-73 0.08461249388907888 1107 74-75 0.4981689681153192 1107 76-77 0.9277666947389527 1107 78-79 1.026382379563909 1107 80-81 0.3422578746713931 1107 82-83 -0.23685967231216765 1107 84-85 0.04591027151757743 1107 86-87 0.788285176802578 1107 88-89 0.8221631853119469 1107 90-91 0.47168689268490027 1107 92-93 0.10603020979394273 1107 94-95 0.5521856690597886 1107 96-97 0.24733768267520162 1107 98-99 -0.2768382575611561 1107 100-101 -1.437820594820895 1108 1 0.5004726695975457 1108 2 0.5149577728492716 1108 3 -0.20656966744647676 1108 4 0.46984990780894265 1108 5 0.1895210571702748 1108 6 0.5422718829001809 1108 7 0.6250166863365934 1108 8 0.618208175574253 1108 9 0.7594855509262004 1108 10-11 0.6451498632297898 1108 12-13 0.3789191138475587 1108 14-15 0.8518269533155518 1108 16-17 0.883968112631841 1108 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3.150657034340913 2313 100-101 2.9554939061207186 2314 1 -0.0974833052452233 2314 2 -0.8133441139431845 2314 3 -0.2240224976351577 2314 4 -0.6718167588577231 2314 5 -0.019126741571867 2314 6 -0.7435771737035921 2314 7 -0.5319015526571178 2314 8 -0.6748735854320387 2314 9 -0.6299798578788298 2314 10-11 -0.3862180612985142 2314 12-13 -0.5964739679134468 2314 14-15 -0.7639749334769022 2314 16-17 -1.0705130194436308 2314 18-19 -1.0344833654662935 2314 20-21 -1.2351598177204934 2314 22-23 -0.8915817337445873 2314 24-25 -0.7356090692568458 2314 26-27 -0.34176195122290665 2314 28-29 -0.5587512942653703 2314 30-31 -0.32171797249969103 2314 32-33 -0.6095267131015518 2314 34-35 -0.4178081754670657 2314 36-37 -0.4767910972874816 2314 38-39 -0.06802605399621342 2314 40-41 -0.450476963272358 2314 42-43 -0.32190485984539663 2314 44-45 0.2760370957937681 2314 46-47 -0.07935959555334193 2314 48-49 0.2732990689296315 2314 50-51 0.25015454287537153 2314 52-53 0.5495973173493454 2314 54-55 1.0707944746921356 2314 56-57 0.6502770025840832 2314 58-59 0.5338468955923261 2314 60-61 0.39988243647827915 2314 62-63 0.3464958214370455 2314 64-65 -0.12064694618312188 2314 66-67 -0.07983892143442972 2314 68-69 0.27037200829095553 2314 70-71 -0.2192229220550459 2314 72-73 -0.19031688464199803 2314 74-75 0.25621981557294404 2314 76-77 0.5123005930440385 2314 78-79 0.1788541309763403 2314 80-81 0.7145742588521848 2314 82-83 0.5596092542415043 2314 84-85 0.7277605540034564 2314 86-87 0.36024845363873936 2314 88-89 1.082685784182008 2314 90-91 1.1821388700860318 2314 92-93 0.7076545035792563 2314 94-95 0.9405331266869084 2314 96-97 0.8741032193983642 2314 98-99 0.3687125898964716 2314 100-101 0.04854946167627716 2315 1 -0.47943774169606357 2315 2 -0.5529124592669241 2315 3 -0.4738426415200472 2315 4 -0.6956177180903396 2315 5 -0.4483233842577192 2315 6 -0.3554476773007167 2315 7 -0.44712937040291933 2315 8 -0.5650414511394715 2315 9 -0.4446681072793126 2315 10-11 -0.3323511548236908 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-2.6422857378912106 2315 78-79 -2.807326924179538 2315 80-81 -3.0237950457041656 2315 82-83 -3.387572999955136 2315 84-85 -2.7157346498334753 2315 86-87 -2.586364256193395 2315 88-89 -2.5482794436357423 2315 90-91 -2.940762808571044 2315 92-93 -2.6832028105694263 2315 94-95 -2.9527222689965456 2315 96-97 -3.1611186031436027 2315 98-99 -2.468247841758206 2315 100-101 -1.3167682841270825 2316 1 -1.7423757783635025 2316 2 -1.3746344365238272 2316 3 -1.308699916989994 2316 4 -4.8269242857394445 2316 5 -2.772621365227778 2316 6 -1.6290610446713387 2316 7 -1.6227617677108839 2316 8 -1.7436907897331082 2316 9 -2.8216465245455 2316 10-11 -2.4495245129114167 2316 12-13 -2.488275043182263 2316 14-15 -2.6667975141220523 2316 16-17 -1.7959162452500834 2316 18-19 -1.7120371289071485 2316 20-21 -2.585428634924803 2316 22-23 -2.5806946369703923 2316 24-25 -2.445958531622445 2316 26-27 -2.820256574878819 2316 28-29 -2.913052369534192 2316 30-31 -2.8211803380910894 2316 32-33 -3.0819729496606882 2316 34-35 -2.3993941969724446 2316 36-37 -2.79843088223371 2316 38-39 -2.764128204533847 2316 40-41 -1.7680844901540738 2316 42-43 -1.7499962576948604 2316 44-45 -1.509446775173977 2316 46-47 -2.175999380499583 2316 48-49 -3.6314052321456387 2316 50-51 -3.0717540592751646 2316 52-53 -2.9834671987796852 2316 54-55 -2.790899073694959 2316 56-57 -3.0744541802116103 2316 58-59 -3.3278466527947685 2316 60-61 -3.4444724022313977 2316 62-63 -3.1209772968425256 2316 64-65 -2.7932275913444116 2316 66-67 -2.820027093477435 2316 68-69 -2.745757023967112 2316 70-71 -2.4548412016249372 2316 72-73 -3.2159889276527487 2316 74-75 -3.7010382489431812 2316 76-77 -3.295360697278543 2316 78-79 -3.1801512453677425 2316 80-81 -4.070371977706952 2316 82-83 -3.4003369823176364 2316 84-85 -2.494685682555687 2316 86-87 -3.13424079367309 2316 88-89 -2.9158357211943375 2316 90-91 -2.6624847858279495 2316 92-93 -3.494899259861608 2316 94-95 -3.327612034603412 2316 96-97 -3.7564075332898064 2316 98-99 -3.6857228939744964 2316 100-101 -3.3652946243452284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 549.0 3 147.0 4 148.0 5 166.0 6 155.0 7 219.0 8 235.0 9 287.0 10 409.0 11 458.0 12 669.0 13 917.0 14 965.0 15 1302.0 16 1796.0 17 2544.0 18 2186.0 19 1317.0 20 1193.0 21 1205.0 22 1258.0 23 1388.0 24 1565.0 25 1653.0 26 1926.0 27 2206.0 28 2547.0 29 3114.0 30 3865.0 31 4728.0 32 6295.0 33 8301.0 34 11655.0 35 17617.0 36 27286.0 37 42145.0 38 41677.0 39 8777.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48320696035974 19.33921500656684 15.261916735427237 21.915661297646192 2 14.518506946817547 20.43867956766679 42.43319241171244 22.609621073803225 3 18.626460264690518 25.973550475242014 30.90464404446332 24.495345215604143 4 12.011620526341488 17.063619940432595 39.50148918509838 31.423270348127534 5 12.928217277678725 37.29526414771756 35.371125711354814 14.405392863248906 6 29.344195041903532 37.15544355232569 18.984059075191936 14.516302330578835 7 26.20096076861489 33.7694545880607 22.598078462770214 17.431506180554198 8 24.992429496634788 34.49414384933233 21.43917711070518 19.0742495433277 9 26.001562652602793 16.681316534817853 21.544584432073442 35.77253638050591 10-11 23.535500755594942 26.794202304137336 29.06782288561586 20.602474054651854 12-13 25.096323353468865 24.77597800555721 29.156798304513647 20.970900336460282 14-15 22.17101119535976 25.577710834549865 28.361659432566633 23.88961853752374 16-17 20.516169351590328 28.847529373854158 28.854120639694557 21.78218063486096 18-19 20.44247852419278 27.018872346190744 31.994619757986754 20.544029371629723 20-21 21.382715928840636 26.105030365756022 32.00121072467731 20.511042980726042 22-23 21.00475455930019 25.958234076619675 32.17894521029406 20.858066153786073 24-25 20.74419013311075 26.10986640112854 32.24750691912314 20.898436546637576 26-27 20.531887892070248 26.403789646319204 32.08093755186114 20.983384909749407 28-29 20.41158008288223 26.52597513533038 32.22351844349316 20.83892633829423 30-31 20.561174185108896 26.33057391372258 32.09289612151859 21.01535577964993 32-33 20.29061906644604 26.48567649226594 32.188721793501344 21.034982647786677 34-35 20.794539069230524 26.621793448152154 31.773997125055214 20.809670357562105 36-37 20.8210900358269 26.25125687007624 31.87837402501049 21.049279069086367 38-39 20.521149584622744 26.61074611712565 31.765133691927723 21.102970606323886 40-41 20.65318195573865 26.6702949129709 31.740972500170837 20.935550631119614 42-43 20.644396067827056 26.693235842517844 31.380751095795468 21.28161699385964 44-45 20.718099905307653 26.786219822915548 31.29484463621543 21.20083563556137 46-47 20.896502240401418 26.918496246473445 30.81088864375177 21.37411286937337 48-49 20.8491155453161 27.027119372095747 30.454137217384513 21.66962786520364 50-51 20.755683981373917 27.423440748948135 30.329129123266007 21.49174614641194 52-53 21.069985161466672 27.433470654847902 30.014838533328124 21.4817056503573 54-55 20.76627959760437 27.308869397138714 30.184553357445832 21.740297647811083 56-57 20.909192967384833 27.421732381854213 29.95353241504535 21.71554223571561 58-59 21.012478431812802 27.118929485755842 30.184479691126477 21.68411239130488 60-61 20.895252831680654 26.962285747756496 30.59459802459615 21.5478633959667 62-63 20.751208083174696 27.096695465417092 30.50056133157612 21.65153511983209 64-65 20.911837635206375 26.77354640946542 30.65450622827912 21.660109727049086 66-67 20.606139006316692 27.05219326935281 30.228845909769888 22.11282181456061 68-69 20.695151271067502 26.921734461001478 30.538815460281427 21.844298807649594 70-71 20.71720636160578 26.758534828937254 30.426100648122155 22.098158161334812 72-73 20.667047446092862 26.615505533506152 30.267082594962975 22.450364425438018 74-75 20.398942597081156 27.044568605482777 30.08814131901983 22.46834747841624 76-77 20.519211661511495 26.95833465569392 30.19815878580444 22.324294896990143 78-79 20.4526592264956 26.79852954099417 30.205190544538503 22.543620687971725 80-81 20.481765905233985 26.944705580719596 30.115374754294503 22.458153759751916 82-83 20.653991801297437 27.181213788860216 29.978245848765923 22.186548561076428 84-85 20.420238629928335 26.84855201234158 29.556572086937845 23.174637270792246 86-87 20.609032484824226 26.713514067644784 29.83995567477587 22.837497772755118 88-89 20.57061821448098 26.483539014293523 29.725210888073107 23.220631883152386 90-91 20.175417183365393 26.55595481042446 30.083827248493844 23.184800757716307 92-93 20.422935795494773 26.74158290874557 29.996606553014676 22.83887474274498 94-95 20.23958877427984 26.723569814157607 30.002538430371345 23.034302981191207 96-97 20.138263359477413 26.963063861365594 29.881631527170555 23.017041251986438 98-99 20.371378691506635 27.018105016420265 29.05055487053021 23.559961421542894 100-101 20.405487239092427 26.907299063321062 29.156695935848724 23.530517761737787 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 40.0 1 37.0 2 43.5 3 50.0 4 43.0 5 49.0 6 74.5 7 177.0 8 326.0 9 388.0 10 342.0 11 312.0 12 320.5 13 325.5 14 362.0 15 376.0 16 389.0 17 425.0 18 440.0 19 457.0 20 513.5 21 608.5 22 721.5 23 852.0 24 1050.5 25 1282.5 26 1663.0 27 2066.5 28 2360.5 29 2698.5 30 3121.5 31 3666.5 32 4104.0 33 4459.5 34 4885.5 35 5289.5 36 5782.0 37 6224.0 38 6436.5 39 6548.0 40 6716.5 41 6857.5 42 6970.5 43 7206.5 44 7481.0 45 7649.0 46 7648.0 47 7760.0 48 8176.0 49 7987.0 50 7214.0 51 6768.5 52 6316.0 53 5892.0 54 5561.5 55 5033.0 56 4486.0 57 3917.5 58 3412.5 59 2961.0 60 2424.5 61 1891.0 62 1452.0 63 1111.0 64 809.5 65 578.0 66 404.0 67 289.0 68 197.0 69 129.5 70 85.5 71 50.0 72 37.0 73 31.0 74 21.5 75 16.5 76 12.0 77 5.0 78 2.5 79 3.5 80 3.0 81 4.0 82 3.5 83 0.5 84 1.0 85 2.0 86 1.5 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02782197838671574 2 0.01561935628727901 3 0.015131251403301542 4 0.03123871257455802 5 0.07760867655241757 6 0.05759637630934135 7 0.018547985591143824 8 0.06442984468502592 9 0.045881859093882094 10-11 0.03343518455245663 12-13 0.046369963977859564 14-15 0.02782197838671574 16-17 0.028066030828704472 18-19 0.024649296640862187 20-21 0.018547985591143824 22-23 0.008785887911594445 24-25 0.0034167341878422837 26-27 0.0 28-29 0.0024405244198873453 30-31 0.0 32-33 4.881048839774691E-4 34-35 2.4405244198873454E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 9.762097679549382E-4 50-51 0.0 52-53 9.762097679549382E-4 54-55 4.881048839774691E-4 56-57 0.0 58-59 2.4405244198873454E-4 60-61 0.0021964719778986112 62-63 0.0019524195359098763 64-65 9.762097679549382E-4 66-67 0.009762097679549381 68-69 0.01293477942540293 70-71 0.025137401524839658 72-73 0.015131251403301542 74-75 0.015863408729267746 76-77 0.030994660132569286 78-79 0.019524195359098762 80-81 0.05198317014360045 82-83 0.0417329675800736 84-85 0.018547985591143824 86-87 0.011958569657447992 88-89 0.012690726983414195 90-91 0.023429034430918514 92-93 0.032947079668479165 94-95 0.01122641233148179 96-97 0.023184981988929782 98-99 0.04710212130382577 100-101 0.05271532746956666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 204874.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.25592309895563 #Duplication Level Percentage of deduplicated Percentage of total 1 91.4317653384769 76.12236023821988 2 5.768122576331617 9.604607392808298 3 1.3356298842341299 3.3359729679139116 4 0.5447517612692826 1.8141524297702412 5 0.2743020704156664 1.1418636040205516 6 0.1638767809291742 0.8186227602446237 7 0.10861060720329324 0.6329733452733781 8 0.07911466846954479 0.5269411803279832 9 0.05621510141274917 0.4212216144197853 >10 0.22590695661966353 3.2450441305449695 >50 0.00526691458708636 0.31572132179848533 >100 0.004681701855187876 0.5763863019870497 >500 5.852127318984845E-4 0.29038566017268097 >1k 0.001170425463796969 1.1537470524981686 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1332 0.6501557054579888 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.505676659800658 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 596 0.29091051085057157 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 251 0.12251432587834472 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4405244198873454E-4 0.0 18-19 0.0 0.0 0.0 4.881048839774691E-4 0.0 20-21 0.0 0.0 0.0 4.881048839774691E-4 0.0 22-23 0.0 0.0 0.0 7.321573259662037E-4 0.0 24-25 0.0 0.0 0.0 0.0026845768618760798 0.0 26-27 0.0 0.0 0.0 0.004392943955797222 0.0 28-29 0.0 0.0 0.0 0.007565625701650771 0.0 30-31 0.0 0.0 0.0 0.015131251403301543 0.0 32-33 0.0 0.0 0.0 0.023429034430918518 0.0 34-35 0.0 0.0 0.0 0.029530345480636877 0.0 36-37 0.0 0.0 0.0 0.036363813856321446 0.0 38-39 0.0 0.0 0.0 0.04197702002206234 0.0 40-41 0.0 0.0 0.0 0.053935589679510336 0.0 42-43 0.0 0.0 0.0 0.07931704364633874 0.0 44-45 0.0 0.0 0.0 0.11690111971260383 0.0 46-47 0.0 0.0 0.0 0.1591221921766549 0.0 48-49 0.0 0.0 0.0 0.2562550640881713 0.0 50-51 0.0 0.0 0.0 0.34533420541405935 0.0 52-53 0.0 0.0 0.0 0.4217226197565333 0.0 54-55 0.0 0.0 0.0 0.5962201157784786 0.0 56-57 0.0 0.0 0.0 0.7738902935462773 0.0 58-59 0.0 0.0 0.0 0.9896326522643186 4.881048839774691E-4 60-61 0.0 0.0 0.0 1.229292150297256 4.881048839774691E-4 62-63 0.0 0.0 0.0 1.6214844245731523 4.881048839774691E-4 64-65 0.0 0.0 0.0 1.8806681179651883 4.881048839774691E-4 66-67 0.0 0.0 0.0 2.2094067573240137 4.881048839774691E-4 68-69 0.0 0.0 0.0 2.6235637513788963 4.881048839774691E-4 70-71 0.0 0.0 0.0 2.8815271825609887 4.881048839774691E-4 72-73 0.0 0.0 0.0 3.3330242002401476 4.881048839774691E-4 74-75 0.0 0.0 0.0 3.8853148764606535 4.881048839774691E-4 76-77 0.0 0.0 0.0 4.295322979001728 4.881048839774691E-4 78-79 0.0 0.0 0.0 4.639680974647832 4.881048839774691E-4 80-81 0.0 0.0 0.0 5.090201782559037 4.881048839774691E-4 82-83 0.0 0.0 0.0 5.5480441637299025 4.881048839774691E-4 84-85 0.0 0.0 0.0 6.189658033718285 4.881048839774691E-4 86-87 0.0 0.0 0.0 7.193933832501928 4.881048839774691E-4 88-89 0.0 0.0 0.0 8.11547585345139 4.881048839774691E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGTAA 40 5.357249E-4 47.536785 1 GTATCAA 1385 0.0 37.411625 1 GGTATCA 815 0.0 34.99641 1 ACTATCC 60 0.003928512 31.691193 8 ATCAACG 1650 0.0 30.826885 3 TATCAAC 1670 0.0 30.457703 2 CAACGCA 1670 0.0 30.457703 5 TCAACGC 1670 0.0 30.457703 4 TACCTGG 125 2.1248161E-7 30.423546 2 AACGCAG 1700 0.0 29.920212 6 ATATAAC 115 3.8210783E-6 28.935436 3 GAGCTAG 85 6.3601916E-4 27.962816 1 AGGGGGT 70 0.008344167 27.163877 7 CGCCGTG 35 0.008403691 27.130718 58-59 ACGCAGA 1905 0.0 26.700455 7 CGCAGAG 1935 0.0 26.286491 8 CTAGACT 110 9.389792E-5 25.929155 4 GCAGAGT 1965 0.0 25.643253 9 TACATGG 845 0.0 25.31545 2 ATACACT 95 0.001217602 25.019361 4 >>END_MODULE