FastQCFastQC Report
Thu 26 May 2016
SRR936941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204874
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13830.6750490545408397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.521296016087937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6860.3348399504085438No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3730.18206312172359598No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.13959799681755616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.11177601843084042No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC250.0050097957.0161179
ATCGTGG459.593167E-442.2341588
GGTATCA11550.035.7893371
GTATCAA16500.029.3719391
GGGGTAC650.005824954229.2390356
TTGTAAC650.005838875729.2247624
GTAAGGT650.005838875729.2247624
TACCTGG1257.349974E-626.6010272
GTGTAGC950.001221099425.007071
TCAACGC19400.024.7242984
TATCAAC19500.024.6035122
ATCAACG19500.024.5975073
GAGTACT11900.024.34373712-13
CAACGCA20050.023.9344465
ACCTGCC1201.7008714E-423.7567168
GCATATC500.001653813323.7451240-41
AACGCAG20250.023.698066
CCAGAAC1652.4180354E-623.0255683
CATGGGG4100.022.0076714
GTACTTT13250.021.68422114-15
Produced by FastQC (version 0.11.5)