##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936941_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 204874 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4220252447846 33.0 31.0 34.0 31.0 34.0 2 32.546199127268466 34.0 31.0 34.0 31.0 34.0 3 32.64442047307126 34.0 31.0 34.0 31.0 34.0 4 36.24576569013149 37.0 35.0 37.0 35.0 37.0 5 36.178006970137744 37.0 35.0 37.0 35.0 37.0 6 36.226329353651515 37.0 36.0 37.0 35.0 37.0 7 36.187266319786794 37.0 36.0 37.0 35.0 37.0 8 36.16732235422748 37.0 36.0 37.0 35.0 37.0 9 37.89088415318684 39.0 38.0 39.0 35.0 39.0 10-11 37.80770375938381 39.0 38.0 39.0 35.0 39.0 12-13 37.73134463133438 39.0 37.0 39.0 35.0 39.0 14-15 38.9945137011041 40.0 38.0 41.0 36.0 41.0 16-17 38.92522965334791 40.0 38.0 41.0 36.0 41.0 18-19 39.07993449632457 40.0 38.5 41.0 36.0 41.0 20-21 39.095278073352404 40.0 39.0 41.0 36.0 41.0 22-23 39.017571775823185 40.0 38.0 41.0 35.5 41.0 24-25 38.897078204164515 40.0 38.0 41.0 35.0 41.0 26-27 38.735820553120455 40.0 38.0 41.0 35.0 41.0 28-29 38.64857180510948 40.0 38.0 41.0 35.0 41.0 30-31 38.62460341478177 40.0 38.0 41.0 35.0 41.0 32-33 38.435328543397404 40.0 38.0 41.0 34.5 41.0 34-35 38.244752872497244 40.0 38.0 41.0 34.0 41.0 36-37 38.09196628171462 40.0 38.0 41.0 34.0 41.0 38-39 37.91402032468737 40.0 37.0 41.0 33.5 41.0 40-41 37.734463621543 40.0 37.0 41.0 33.0 41.0 42-43 37.480959028476036 40.0 37.0 41.0 33.0 41.0 44-45 37.12948690414596 40.0 37.0 41.0 32.5 41.0 46-47 36.660249714458644 40.0 36.5 41.0 31.0 41.0 48-49 36.19726514833508 39.5 36.0 41.0 30.0 41.0 50-51 35.858027372921896 39.0 35.5 41.0 28.5 41.0 52-53 35.61390903677382 39.0 35.0 40.0 28.0 41.0 54-55 35.26389878657126 39.0 35.0 40.0 27.0 41.0 56-57 34.989618009117805 38.5 35.0 40.0 26.0 41.0 58-59 34.66777141072073 38.0 34.0 40.0 26.0 41.0 60-61 34.35213350644787 38.0 34.0 40.0 25.0 41.0 62-63 33.99935814207757 37.5 34.0 40.0 24.0 41.0 64-65 33.62813485361734 37.0 33.5 39.5 23.0 41.0 66-67 33.26413551743999 36.0 33.0 39.0 22.0 40.0 68-69 32.95510167224733 36.0 33.0 39.0 21.5 40.0 70-71 32.477905932426765 35.5 32.0 38.5 19.5 40.0 72-73 31.9501888965901 35.0 32.0 38.0 17.5 40.0 74-75 31.470828411609087 35.0 31.0 37.0 14.0 39.0 76-77 30.06242129308746 33.5 29.5 35.5 12.0 38.5 78-79 30.72750812694632 34.0 31.0 36.0 9.0 39.0 80-81 30.342295752511298 34.0 31.0 36.0 7.0 37.5 82-83 30.09143912843992 34.0 30.0 35.5 6.0 37.0 84-85 29.78406483985279 34.0 30.0 35.0 2.0 37.0 86-87 29.51211720374474 34.0 30.0 35.0 2.0 36.0 88-89 29.14924294932495 34.0 29.5 35.0 2.0 36.0 90-91 28.751876763278894 34.0 29.0 35.0 2.0 35.5 92-93 28.319425598172536 34.0 29.0 35.0 2.0 35.0 94-95 27.754820035729278 33.5 28.0 35.0 2.0 35.0 96-97 27.106511807257142 33.0 26.5 35.0 2.0 35.0 98-99 26.385085467165183 33.0 25.0 34.0 2.0 35.0 100-101 23.10469605708875 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16354719462582779 1101 2 0.03331319419419998 1101 3 0.06866364562521454 1101 4 0.057672422563669556 1101 5 0.0706113872517875 1101 6 0.04691199952355163 1101 7 0.0710560302611043 1101 8 0.16183240801084509 1101 9 0.17703432385432905 1101 10-11 0.09860932063293859 1101 12-13 0.08758920591260022 1101 14-15 0.0924938802680586 1101 16-17 0.24354860157714597 1101 18-19 -0.030846894898523658 1101 20-21 0.05433159916550068 1101 22-23 0.14977281730655534 1101 24-25 0.2509171163214958 1101 26-27 0.19516185448893708 1101 28-29 0.12514374986538002 1101 30-31 0.43027865463080417 1101 32-33 0.37699578454143534 1101 34-35 0.5815505081035539 1101 36-37 0.41846306305901493 1101 38-39 0.14573262039024826 1101 40-41 0.28052176106155713 1101 42-43 0.4783922643479741 1101 44-45 0.49877538116083997 1101 46-47 0.7557656482293211 1101 48-49 0.8550572969708199 1101 50-51 0.811618857460239 1101 52-53 0.891622003763267 1101 54-55 0.9699262151569528 1101 56-57 0.9164045438745205 1101 58-59 0.7810966990238626 1101 60-61 0.751586483412666 1101 62-63 0.6546607240601148 1101 64-65 0.8505590878955331 1101 66-67 0.4448353422579885 1101 68-69 0.4525769798031618 1101 70-71 0.7154720525527694 1101 72-73 0.6924471895383668 1101 74-75 0.7171951089128008 1101 76-77 0.3172356483190164 1101 78-79 0.609110074025736 1101 80-81 0.6039434604562786 1101 82-83 0.7522753941662295 1101 84-85 0.7379798030377671 1101 86-87 0.4314026265733233 1101 88-89 0.136947861529368 1101 90-91 0.24398194699713827 1101 92-93 0.34960717279236064 1101 94-95 0.5147917829682207 1101 96-97 0.4942051937113554 1101 98-99 0.3345286454259728 1101 100-101 0.9144600413895283 1102 1 0.13236253402940434 1102 2 0.14285214490961806 1102 3 0.25104067506106986 1102 4 0.2110230994437572 1102 5 0.31207691695261275 1102 6 0.13614936523150334 1102 7 -0.08883701786724174 1102 8 0.08599293217136506 1102 9 0.1847088432935564 1102 10-11 0.23769493030678035 1102 12-13 0.14628265872370605 1102 14-15 0.22554538096661503 1102 16-17 0.20228059560325562 1102 18-19 0.07778874609005726 1102 20-21 -0.08147559840640639 1102 22-23 0.11760776455326294 1102 24-25 0.4858980865965776 1102 26-27 0.10445028730677564 1102 28-29 -0.22798531502839126 1102 30-31 0.0624846574250526 1102 32-33 -0.03714161489008205 1102 34-35 0.18662349552948143 1102 36-37 -0.03818288770458622 1102 38-39 0.14979871857399019 1102 40-41 0.2912651247460971 1102 42-43 0.3536993896550982 1102 44-45 0.04579566347523922 1102 46-47 0.031031823495489164 1102 48-49 -0.16160936969584583 1102 50-51 -0.03525930412380518 1102 52-53 0.30272428251260664 1102 54-55 -0.0677719107764645 1102 56-57 -0.18885874080052645 1102 58-59 0.017530191339709233 1102 60-61 0.055628493337032126 1102 62-63 0.38389905329844254 1102 64-65 0.09692898720660992 1102 66-67 0.3243265982198267 1102 68-69 0.2064383277821591 1102 70-71 0.27137367610144736 1102 72-73 0.434115060617998 1102 74-75 -0.00753630699508534 1102 76-77 -0.1293775424118202 1102 78-79 0.010902240523787299 1102 80-81 0.26845422790822226 1102 82-83 0.21714025903109402 1102 84-85 0.04147019182227396 1102 86-87 -0.0012538266713626456 1102 88-89 0.05237157401190373 1102 90-91 0.174528268719925 1102 92-93 0.1565590444501126 1102 94-95 -0.3681489117371797 1102 96-97 -0.19204522783318012 1102 98-99 -0.13031454000544684 1102 100-101 0.053386668551450356 1103 1 0.2505408107585154 1103 2 0.21394680794039544 1103 3 0.32842141664126245 1103 4 0.12057439738617148 1103 5 0.23538675864967473 1103 6 0.08541805524814805 1103 7 -0.2382154929177034 1103 8 0.05318137286974434 1103 9 0.24725089804441325 1103 10-11 0.2913036706120522 1103 12-13 0.20456611659811585 1103 14-15 0.26652884238248475 1103 16-17 0.49315781343801035 1103 18-19 0.2979431534279442 1103 20-21 0.2330142553315966 1103 22-23 0.44118144447676855 1103 24-25 0.1730727156294094 1103 26-27 0.3598775635755018 1103 28-29 0.3178410829600651 1103 30-31 0.35390194679715137 1103 32-33 0.46006063747383763 1103 34-35 0.7585238390503903 1103 36-37 0.6845390728436271 1103 38-39 0.546884102998682 1103 40-41 0.6238783626963027 1103 42-43 0.7624892752661836 1103 44-45 0.7257457289921163 1103 46-47 1.0516519318639368 1103 48-49 1.0271323521584534 1103 50-51 1.3098021400806061 1103 52-53 1.0827203869146373 1103 54-55 1.1493457008678334 1103 56-57 1.4768812457419571 1103 58-59 1.4855862879505395 1103 60-61 1.1883444334156508 1103 62-63 1.4642723224134286 1103 64-65 1.410427234187523 1103 66-67 1.2732942647566432 1103 68-69 1.4822112498347835 1103 70-71 1.750496812411967 1103 72-73 1.8794527381333204 1103 74-75 1.7977988915033443 1103 76-77 1.4052047432417503 1103 78-79 1.6617769793416493 1103 80-81 1.614011475491182 1103 82-83 1.8131667490973165 1103 84-85 1.3121788365083233 1103 86-87 1.215607029651899 1103 88-89 0.9589214907877626 1103 90-91 1.2508908426477525 1103 92-93 1.475103861534084 1103 94-95 1.5188876006401983 1103 96-97 1.3259276446391048 1103 98-99 1.2036444499722414 1103 100-101 1.285998135775305 1104 1 -0.14377108255546034 1104 2 0.08684892170252567 1104 3 -0.13935807472527273 1104 4 -0.1371443119790925 1104 5 -0.2256492569540569 1104 6 -0.3251477314402891 1104 7 -0.1883047003325089 1104 8 -0.2921722816102843 1104 9 -0.2983204830073234 1104 10-11 -0.13432909723699282 1104 12-13 -0.20653642656920113 1104 14-15 -0.4597707130924249 1104 16-17 -0.28624283369374837 1104 18-19 -0.04864277296837827 1104 20-21 -0.3291436569124997 1104 22-23 -0.05343587083364554 1104 24-25 -0.05804252825458889 1104 26-27 -0.03408657201976695 1104 28-29 -0.33648774464283093 1104 30-31 0.22304676520713684 1104 32-33 0.06211240801422235 1104 34-35 0.14560085982513726 1104 36-37 -0.11583287671637521 1104 38-39 0.037328325684583774 1104 40-41 0.21881621990395672 1104 42-43 -0.09534585733535295 1104 44-45 -0.18473689824185158 1104 46-47 -0.15410723835589835 1104 48-49 0.06099337003018235 1104 50-51 0.3459765027767929 1104 52-53 0.2218083544975613 1104 54-55 0.02620166869267848 1104 56-57 0.18102258657475545 1104 58-59 0.3071487785006042 1104 60-61 0.41010801864653246 1104 62-63 0.4799970192594216 1104 64-65 0.517423212293906 1104 66-67 0.4375417472931602 1104 68-69 0.624686075199925 1104 70-71 0.46110947079292686 1104 72-73 0.4105392542057693 1104 74-75 0.5467026986943644 1104 76-77 0.7934913560815708 1104 78-79 1.1903762717302904 1104 80-81 0.9520244181262782 1104 82-83 0.9796973366566668 1104 84-85 1.073806040233869 1104 86-87 0.4945767596104709 1104 88-89 0.7901380380072105 1104 90-91 0.8579397404069837 1104 92-93 0.4423012367192989 1104 94-95 0.8433984718488823 1104 96-97 0.11175424961657754 1104 98-99 0.13824331900366005 1104 100-101 0.6107206855953784 1105 1 0.26317421017640896 1105 2 0.09208406697781157 1105 3 0.2313192949050702 1105 4 0.07377387433390936 1105 5 0.10146007032977167 1105 6 0.18943796497945442 1105 7 0.30417704936937895 1105 8 0.14621296691369423 1105 9 0.21466558568941707 1105 10-11 0.1869779440334085 1105 12-13 0.2903951966166787 1105 14-15 0.22734210428825463 1105 16-17 0.05908770756211368 1105 18-19 -0.06847815673361168 1105 20-21 -0.07278150119601179 1105 22-23 -0.18329655780104304 1105 24-25 -0.2056445408771026 1105 26-27 7.853123416907692E-4 1105 28-29 0.09006025873093648 1105 30-31 0.1970600857434519 1105 32-33 0.35923598652686195 1105 34-35 0.6510977623641807 1105 36-37 0.5012598726074131 1105 38-39 0.6957641806128905 1105 40-41 0.7637948430607437 1105 42-43 1.4270925569973159 1105 44-45 0.9057874888098851 1105 46-47 1.2452761778672325 1105 48-49 1.35590655387103 1105 50-51 1.5098801761037208 1105 52-53 1.2020311390131653 1105 54-55 1.51256120683653 1105 56-57 1.7449349851438143 1105 58-59 1.5423522220628314 1105 60-61 1.7166694007248822 1105 62-63 1.3702916416018596 1105 64-65 1.0161871363261241 1105 66-67 1.7027454816967875 1105 68-69 1.5251310625483327 1105 70-71 1.3979532598747397 1105 72-73 1.2899854921722032 1105 74-75 1.6270476365997233 1105 76-77 1.2327345867478918 1105 78-79 0.976040698918144 1105 80-81 1.1664327012894695 1105 82-83 1.3954842080756826 1105 84-85 0.9861464410133252 1105 86-87 0.6205401717618244 1105 88-89 1.091856910705932 1105 90-91 0.5613687605622921 1105 92-93 1.1037473001808955 1105 94-95 1.1901977621958544 1105 96-97 0.6923173185750748 1105 98-99 -0.02878689941823609 1105 100-101 0.5277727171225841 1106 1 -0.20676402568539487 1106 2 0.0858111466921514 1106 3 0.22697650393807578 1106 4 -0.11161504672379863 1106 5 -0.03417976753936358 1106 6 -0.20787915526760514 1106 7 -0.053186393981320634 1106 8 0.12387172005015401 1106 9 0.08399583081583728 1106 10-11 -0.0711285991049877 1106 12-13 -0.023057804734406773 1106 14-15 0.16159707437125803 1106 16-17 -0.05440389637535503 1106 18-19 0.17582796177632787 1106 20-21 -0.151217131383234 1106 22-23 -0.2478111302773982 1106 24-25 0.01709238249675593 1106 26-27 -0.06667270734562436 1106 28-29 -0.5022723025506721 1106 30-31 -0.5720964477442934 1106 32-33 -0.17439651685086943 1106 34-35 -0.1433675918145454 1106 36-37 -0.3612666666707156 1106 38-39 -0.2846932600356311 1106 40-41 -0.33997373443393997 1106 42-43 -0.044027883072168095 1106 44-45 -0.0025554951700357265 1106 46-47 0.00830455076821579 1106 48-49 -0.22979118787765884 1106 50-51 0.03381377965516208 1106 52-53 0.12513961228087567 1106 54-55 0.13833325856399625 1106 56-57 0.06292379039377494 1106 58-59 -0.6388869209597487 1106 60-61 -0.3015461947200109 1106 62-63 -0.3774645830651977 1106 64-65 -0.15173411439766937 1106 66-67 -0.6219746495520013 1106 68-69 0.07876453099071767 1106 70-71 -0.36075644867572976 1106 72-73 0.12551434760552027 1106 74-75 0.4055652972829904 1106 76-77 -0.1948855638021989 1106 78-79 0.20698067189633562 1106 80-81 -0.2946028130899947 1106 82-83 -0.21467792278708586 1106 84-85 0.34160388166184674 1106 86-87 -0.21515756998687507 1106 88-89 -0.07396532438382053 1106 90-91 -0.018876365861174804 1106 92-93 0.25036474854993784 1106 94-95 0.5962004643769028 1106 96-97 0.8146392641454305 1106 98-99 0.39820595910328294 1106 100-101 0.8927360446655292 1107 1 -0.005043429897000351 1107 2 0.262493221679307 1107 3 0.2932322820582627 1107 4 0.13466127582371 1107 5 0.15187570241440795 1107 6 0.1377808344601803 1107 7 0.337157725176354 1107 8 0.209259496115898 1107 9 0.30561884057443933 1107 10-11 0.44774659349901924 1107 12-13 0.4016982301911156 1107 14-15 0.35837416733478733 1107 16-17 0.32425557974174524 1107 18-19 0.2007380799067917 1107 20-21 0.24407049059930586 1107 22-23 0.29700140182326606 1107 24-25 0.2527112833698979 1107 26-27 0.42831958849412644 1107 28-29 0.3363897416198469 1107 30-31 0.6573591249316166 1107 32-33 0.5230867908019334 1107 34-35 0.8550273748007626 1107 36-37 0.8053999999959487 1107 38-39 1.0230879418133654 1107 40-41 0.587286152571707 1107 42-43 0.6225745822591051 1107 44-45 0.4304825120205109 1107 46-47 0.983266029966984 1107 48-49 0.9273736811516144 1107 50-51 1.1941887154538264 1107 52-53 0.7456455188037339 1107 54-55 1.0750949432070414 1107 56-57 1.082055788339325 1107 58-59 0.9377694734932476 1107 60-61 1.2797968972163005 1107 62-63 1.084207219708297 1107 64-65 0.43840456048676657 1107 66-67 0.9145559102836387 1107 68-69 1.0134717729013474 1107 70-71 0.7678388261059865 1107 72-73 0.6739863558232919 1107 74-75 0.8269828627683502 1107 76-77 0.6937893206353998 1107 78-79 0.8080335737966955 1107 80-81 1.0344290307723583 1107 82-83 1.3273097505565197 1107 84-85 0.8579752221857291 1107 86-87 0.7428547566695194 1107 88-89 1.0348559390984597 1107 90-91 1.086927434857877 1107 92-93 1.2233359863516817 1107 94-95 1.757859426883325 1107 96-97 1.725271005285645 1107 98-99 1.2297416550457605 1107 100-101 1.0792409239670206 1108 1 0.40029715213050565 1108 2 0.4214315011804288 1108 3 0.28443791045608435 1108 4 0.21828775487711738 1108 5 -0.07405969892872832 1108 6 0.26010254856315385 1108 7 0.46539565290260754 1108 8 0.5268162597871466 1108 9 0.34271509896906593 1108 10-11 0.3405352087852336 1108 12-13 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-1.3835694299556671 2316 90-91 -1.4523563267604942 2316 92-93 -1.1342637949495753 2316 94-95 -1.74446424725555 2316 96-97 -1.350316747584774 2316 98-99 -1.412986611766705 2316 100-101 -0.6018142974654559 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 4.0 6 8.0 7 17.0 8 46.0 9 90.0 10 180.0 11 300.0 12 469.0 13 697.0 14 885.0 15 1277.0 16 1795.0 17 2802.0 18 2586.0 19 1479.0 20 1162.0 21 1113.0 22 1177.0 23 1227.0 24 1292.0 25 1467.0 26 1728.0 27 2066.0 28 2542.0 29 3183.0 30 4033.0 31 5138.0 32 6545.0 33 8768.0 34 12753.0 35 18996.0 36 30671.0 37 48734.0 38 37011.0 39 2633.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37867661419475 20.235049412132337 15.283094410374595 21.10317956329831 2 15.409021462705072 21.53592330634021 40.576081067228984 22.478974163725734 3 19.481730234192725 26.274685904507162 30.655915343088925 23.587668518211196 4 12.72294190575671 18.327850288469985 38.08438357234202 30.864824233431282 5 12.978708864960902 38.700860040805566 33.9711237150639 14.349307379169634 6 30.099475775354605 37.813973466618506 17.672325429288247 14.414225328738638 7 26.742290383357574 34.58076671515175 21.215478782080694 17.46146411940998 8 25.820748362408114 35.15965910754903 19.917607895584602 19.10198463445825 9 26.781338774075774 17.0948973515429 20.783505959760635 35.34025791462069 10-11 24.12251154525839 27.92902961248938 27.379348388545544 20.56911045370669 12-13 25.821236467292092 25.50714097445259 28.056756835908903 20.61486572234642 14-15 22.591446450013176 26.590489764440584 27.261633979909604 23.556429805636636 16-17 20.92749690053399 29.944014369807785 27.636986635688277 21.491502093969952 18-19 20.779021098488084 27.913458370449913 31.25187616688428 20.05564436417772 20-21 21.958505494934606 26.835976873195506 31.226311255158745 19.979206376711144 22-23 21.43795028994279 26.94857176328172 31.145664525450535 20.46781342132495 24-25 21.334059072924624 27.017283504638794 31.39417667202835 20.25448075040823 26-27 21.173174267681325 27.330703933848476 31.1918208754143 20.304300923055894 28-29 20.82270680545217 27.54737702717544 31.28018645743084 20.34972970994155 30-31 21.137038691238885 27.28204602531905 31.166301793127687 20.414613490314377 32-33 21.20253627963508 27.481976111330763 30.903708259504945 20.41177934952921 34-35 21.299432822124817 27.433690951511664 31.00344602048088 20.263430205882642 36-37 21.828029426553215 27.079946692441748 30.923997676337205 20.16802620466783 38-39 21.296958483952775 27.602105946589795 30.821070205542146 20.279865363915285 40-41 21.181865661806697 27.665701831884455 30.749837702749534 20.402594803559314 42-43 21.15056083251169 27.83662153323506 30.420648789011786 20.592168845241467 44-45 21.475638685240682 27.783662153323508 30.153899469918095 20.58679969151771 46-47 21.65561103708151 28.09911506152592 29.552698501022583 20.692575400369986 48-49 21.78479035485918 28.19861375506419 29.117733196661298 20.89886269341534 50-51 21.528882857728618 28.600716579941622 28.94899981450928 20.921400747820485 52-53 21.820728838212755 28.842605699112628 28.61026777433935 20.72639768833527 54-55 21.63600819077976 28.5769374391366 28.694332107319713 21.092722262763925 56-57 21.776758148559434 28.680244548305613 28.701233724236396 20.841763578898558 58-59 21.68674404756094 28.543397405234437 28.672013042162497 21.097845505042123 60-61 21.45318670161617 28.422830421688218 29.225546330058037 20.898436546637576 62-63 21.268868903071496 28.40884962231686 29.35873186327748 20.963549611334155 64-65 21.40486347706395 28.55560002733387 29.066645840858286 20.972890654743892 66-67 21.156512061542667 28.49228276043853 28.85398260326262 21.497222574756183 68-69 21.427229130312757 28.315171631136437 29.001208068432803 21.256391170118 70-71 21.390969461345282 28.678708361094635 28.783896247159806 21.146425930400277 72-73 21.39363706473247 28.23686753809659 28.681287034958068 21.688208362212873 74-75 21.360689985063992 28.508009801146073 28.31130353290315 21.81999668088679 76-77 21.167156398566924 28.617833400040997 28.490926130206855 21.724084071185214 78-79 21.341703011473324 28.335281756656165 28.253521195510245 22.06949403636026 80-81 21.371879042296147 28.519268786762016 28.183681936885268 21.925170234056573 82-83 21.34566518010794 28.823372901669874 27.971225690859253 21.859736227362934 84-85 21.358730406681865 28.766725407975475 27.72036533514276 22.154178850199898 86-87 21.039310348194107 28.424706014439337 27.684433531683077 22.851550105683476 88-89 21.106387340511727 28.478479455665433 27.72191688550036 22.69321631832248 90-91 21.144177828107306 28.51512163692452 27.577218946464132 22.763481588504042 92-93 21.056215313877377 28.381485115168907 27.73083812898723 22.831461441966486 94-95 20.959223717992522 29.102033444946652 27.29531321690405 22.64342962015678 96-97 21.18763407925533 29.154901046763914 27.145085041941336 22.51237983203942 98-99 21.074006008732432 29.230457586207738 26.413528678152083 23.282007726907747 100-101 20.89221127234921 29.592479243238497 26.033933217163803 23.481376267248493 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 104.0 1 74.0 2 52.5 3 68.5 4 65.5 5 63.0 6 82.5 7 164.0 8 285.0 9 353.5 10 339.0 11 319.0 12 335.5 13 367.5 14 391.0 15 409.5 16 453.5 17 476.5 18 496.0 19 540.0 20 586.5 21 644.5 22 728.5 23 854.5 24 1037.5 25 1279.5 26 1561.0 27 1918.5 28 2252.0 29 2622.0 30 3129.0 31 3609.5 32 4051.0 33 4358.5 34 4776.0 35 5172.5 36 5634.0 37 6127.5 38 6302.0 39 6455.5 40 6549.0 41 6736.5 42 6872.0 43 7031.5 44 7372.0 45 7417.5 46 7433.0 47 7699.0 48 8143.0 49 7869.0 50 7120.5 51 6732.5 52 6337.0 53 6073.5 54 5790.0 55 5215.0 56 4579.0 57 4035.5 58 3735.0 59 3308.5 60 2644.5 61 2095.5 62 1624.5 63 1194.5 64 819.0 65 587.5 66 412.5 67 310.0 68 226.0 69 138.5 70 101.0 71 67.0 72 35.0 73 22.0 74 17.5 75 17.5 76 11.5 77 3.5 78 2.0 79 2.5 80 1.5 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03221492234251296 2 0.0034167341878422837 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013666936751369135 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.321573259662036E-4 20-21 0.0017083670939211418 22-23 0.0029286293038648143 24-25 0.012446674541425463 26-27 0.0053691537237521595 28-29 7.321573259662036E-4 30-31 0.004148891513808487 32-33 0.0034167341878422837 34-35 0.0 36-37 0.012202622099436728 38-39 0.012446674541425463 40-41 0.0014643146519324071 42-43 0.0 44-45 0.0 46-47 4.881048839774691E-4 48-49 0.0019524195359098763 50-51 0.0058572586077296285 52-53 0.0 54-55 0.004636996397785956 56-57 0.0031726817458535487 58-59 0.0 60-61 0.0034167341878422837 62-63 0.0031726817458535487 64-65 0.0 66-67 0.0039048390718197526 68-69 7.321573259662036E-4 70-71 0.0012202622099436726 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.003660786629831018 80-81 0.0043929439557972225 82-83 0.01879203803313256 84-85 0.01025020256352685 86-87 0.008297783027616974 88-89 0.0 90-91 9.762097679549382E-4 92-93 4.881048839774691E-4 94-95 0.0 96-97 0.0021964719778986112 98-99 0.0026845768618760798 100-101 4.881048839774691E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 204874.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.78648157084967 #Duplication Level Percentage of deduplicated Percentage of total 1 83.593695607812 59.173035935815996 2 10.767538800480548 15.2439237372725 3 2.616658980273173 5.556722480529158 4 1.089524208172316 3.084943411311369 5 0.5660389345437937 2.0033952304233815 6 0.3375974436149498 1.433840113248936 7 0.2178615310074745 1.079515587476038 8 0.1666285449651583 0.943603874188294 9 0.11070345425256778 0.7052677221850909 >10 0.49359994303852966 6.025021861529565 >50 0.026441924382142552 1.2519799304117212 >100 0.01077263585939141 1.3240762276225844 >500 9.793305326719463E-4 0.47555826814088636 >1k 0.0019586610653438926 1.6991156198444788 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1383 0.6750490545408397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1068 0.521296016087937 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 686 0.3348399504085438 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 373 0.18206312172359598 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.13959799681755616 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 229 0.11177601843084042 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4405244198873454E-4 0.0 18-19 0.0 0.0 0.0 4.881048839774691E-4 0.0 20-21 0.0 0.0 0.0 4.881048839774691E-4 0.0 22-23 0.0 0.0 0.0 7.321573259662037E-4 0.0 24-25 0.0 0.0 0.0 0.0026845768618760798 0.0 26-27 0.0 0.0 0.0 0.004392943955797222 0.0 28-29 0.0 0.0 0.0 0.007565625701650771 0.0 30-31 0.0 0.0 0.0 0.014155041635346604 0.0 32-33 0.0 0.0 0.0 0.02318498198892978 0.0 34-35 0.0 0.0 0.0 0.029774397922625616 0.0 36-37 0.0 0.0 0.0 0.03660786629831018 0.0 38-39 0.0 0.0 0.0 0.042221072464051074 0.0 40-41 0.0 0.0 0.0 0.05442369456348781 0.0 42-43 0.0 0.0 0.0 0.08029325341429366 0.0 44-45 0.0 0.0 0.0 0.11860948680652499 0.0 46-47 0.0 0.0 0.0 0.161806769038531 0.0 48-49 0.0 0.0 0.0 0.2572312738561262 0.0 50-51 0.0 0.0 0.0 0.3441139432041157 0.0 52-53 0.0 0.0 0.0 0.4212345148725558 0.0 54-55 0.0 0.0 0.0 0.5962201157784786 2.4405244198873454E-4 56-57 0.0 0.0 0.0 0.7748665033142321 4.881048839774691E-4 58-59 0.0 0.0 0.0 0.9925612815681835 4.881048839774691E-4 60-61 0.0 0.0 0.0 1.231732674717143 4.881048839774691E-4 62-63 0.0 0.0 0.0 1.6290500502748029 4.881048839774691E-4 64-65 0.0 0.0 0.0 1.8906742680867263 4.881048839774691E-4 66-67 0.0 0.0 0.0 2.218192645235608 7.321573259662037E-4 68-69 0.0 0.0 0.0 2.632105586848502 9.762097679549382E-4 70-71 0.0 0.0 0.0 2.8920214375665045 9.762097679549382E-4 72-73 0.0 0.0 0.0 3.3435184552456634 9.762097679549382E-4 74-75 0.0 0.0 0.0 3.8960531839081582 9.762097679549382E-4 76-77 0.0 0.0 0.0 4.305573181565254 9.762097679549382E-4 78-79 0.0 0.0 0.0 4.6509073869793145 9.762097679549382E-4 80-81 0.0 0.0 0.0 5.100451985122563 9.762097679549382E-4 82-83 0.0 0.0 0.0 5.564639729785136 9.762097679549382E-4 84-85 0.0 0.0 0.0 6.2152835401271025 9.762097679549382E-4 86-87 0.0 0.0 0.0 7.227369017054384 0.0012202622099436726 88-89 0.0 0.0 0.0 8.153792086843621 0.0014643146519324071 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGTC 25 0.00500979 57.016117 9 ATCGTGG 45 9.593167E-4 42.234158 8 GGTATCA 1155 0.0 35.789337 1 GTATCAA 1650 0.0 29.371939 1 GGGGTAC 65 0.0058249542 29.239035 6 TTGTAAC 65 0.0058388757 29.224762 4 GTAAGGT 65 0.0058388757 29.224762 4 TACCTGG 125 7.349974E-6 26.601027 2 GTGTAGC 95 0.0012210994 25.00707 1 TCAACGC 1940 0.0 24.724298 4 TATCAAC 1950 0.0 24.603512 2 ATCAACG 1950 0.0 24.597507 3 GAGTACT 1190 0.0 24.343737 12-13 CAACGCA 2005 0.0 23.934446 5 ACCTGCC 120 1.7008714E-4 23.756716 8 GCATATC 50 0.0016538133 23.74512 40-41 AACGCAG 2025 0.0 23.69806 6 CCAGAAC 165 2.4180354E-6 23.025568 3 CATGGGG 410 0.0 22.007671 4 GTACTTT 1325 0.0 21.684221 14-15 >>END_MODULE