FastQCFastQC Report
Thu 26 May 2016
SRR936940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217938
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.6382549165358955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.47444686103387207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.27438996411823546No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.1192999843992328No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13750.037.6726651
GGTATCA8500.032.9864541
TATCAAC16500.030.2487642
ATCAACG16550.030.1573773
TCAACGC16600.030.0665424
AACGCAG16550.029.8701636
CAACGCA16650.029.6907635
CCCAATG1004.911081E-528.5202622
CCAAGAC1004.911081E-528.5202623
GTACATG8550.027.235321
ACGCAGA18500.027.229317
GTGTAAG1056.858087E-527.1559141
CCGGAAC350.00837567427.1496864-65
CGCAGAG18700.026.9380868
TACATGG9150.025.9747372
TGGGATA1309.91763E-625.5951066
GCAGAGT19250.025.180949
GTCTTAG950.00121993725.012031
ACATGGG8900.024.5680163
GAGTACT11200.023.97358312-13
Produced by FastQC (version 0.11.5)