##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936940_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 217938 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93123273591572 33.0 31.0 34.0 30.0 34.0 2 32.02332314695005 33.0 31.0 34.0 30.0 34.0 3 32.039231341023594 34.0 31.0 34.0 30.0 34.0 4 35.62577430278336 37.0 35.0 37.0 33.0 37.0 5 35.614344446585726 37.0 35.0 37.0 33.0 37.0 6 35.68125338398994 37.0 35.0 37.0 35.0 37.0 7 35.63597903991043 37.0 35.0 37.0 33.0 37.0 8 35.5991061678092 37.0 35.0 37.0 33.0 37.0 9 37.26590131138214 39.0 37.0 39.0 34.0 39.0 10-11 37.14508713487322 39.0 37.0 39.0 34.0 39.0 12-13 37.08654296175976 39.0 37.0 39.0 33.5 39.0 14-15 38.27917572887702 40.0 38.0 41.0 33.5 41.0 16-17 38.2351838596298 40.0 38.0 41.0 33.0 41.0 18-19 38.20825877084309 40.0 38.0 41.0 33.5 41.0 20-21 38.15605585074654 40.0 38.0 41.0 34.0 41.0 22-23 38.05494223127678 40.0 38.0 41.0 33.0 41.0 24-25 38.02025576081271 40.0 38.0 41.0 33.0 41.0 26-27 38.018211601464635 40.0 38.0 41.0 33.0 41.0 28-29 37.92563940203177 40.0 38.0 41.0 33.0 41.0 30-31 37.75338857840303 40.0 37.5 41.0 33.0 41.0 32-33 37.50501748203618 40.0 37.0 41.0 33.0 41.0 34-35 37.31980655048684 40.0 36.5 41.0 32.0 41.0 36-37 37.140971285411446 40.0 36.0 41.0 31.5 41.0 38-39 36.952759500408376 39.5 36.0 41.0 31.0 41.0 40-41 36.74596674283512 39.0 36.0 41.0 31.0 41.0 42-43 36.49638429278052 39.0 35.5 41.0 30.0 41.0 44-45 36.186068973744824 39.0 35.0 41.0 30.0 41.0 46-47 35.774676742926886 39.0 35.0 40.0 28.5 41.0 48-49 35.47083344804486 39.0 35.0 40.5 27.0 41.0 50-51 34.778299332837776 38.5 34.0 40.0 25.0 40.5 52-53 34.956113665354366 38.5 35.0 40.0 26.0 40.5 54-55 35.32594361699199 39.0 35.0 40.5 26.0 41.0 56-57 35.194881112977086 39.0 35.0 41.0 26.0 41.0 58-59 34.980070019913924 39.0 35.0 41.0 25.5 41.0 60-61 34.71239756260955 38.5 35.0 40.5 24.5 41.0 62-63 34.432157310794814 38.0 34.0 40.0 23.5 41.0 64-65 34.1114697758078 38.0 34.0 40.0 22.5 41.0 66-67 33.74175453569364 37.0 34.0 40.0 22.0 41.0 68-69 33.34312281474548 37.0 33.5 39.5 20.0 41.0 70-71 32.899641182354614 36.0 33.0 39.0 18.0 41.0 72-73 32.51199423689306 36.0 33.0 39.0 17.5 40.5 74-75 32.11004276445595 35.0 32.0 38.5 13.5 40.0 76-77 31.6590177022823 35.0 32.0 37.0 9.0 39.5 78-79 31.187828648514717 35.0 31.0 37.0 7.0 39.0 80-81 30.74572355440538 35.0 31.0 36.0 2.0 39.0 82-83 30.325234699777 35.0 31.0 36.0 2.0 37.5 84-85 29.94180684414834 34.5 30.5 35.5 2.0 37.0 86-87 29.574190824913508 34.0 30.0 35.0 2.0 37.0 88-89 29.194050601547232 34.0 30.0 35.0 2.0 36.0 90-91 28.795535886353 34.0 29.0 35.0 2.0 36.0 92-93 28.409907863704355 34.0 29.0 35.0 2.0 35.5 94-95 28.024396846809644 34.0 29.0 35.0 2.0 35.0 96-97 27.555981517679342 34.0 27.5 35.0 2.0 35.0 98-99 26.989558957134598 34.0 26.5 35.0 2.0 35.0 100-101 24.95112600831429 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24334972913323938 1101 2 0.2847498483274862 1101 3 0.24406163566892403 1101 4 0.14690300019924507 1101 5 0.15487379588449102 1101 6 0.26640476042967265 1101 7 0.3473203927087667 1101 8 0.11280874151531606 1101 9 0.027474745270531287 1101 10-11 0.06995648026982337 1101 12-13 0.1599021445500739 1101 14-15 0.357258381100408 1101 16-17 0.1722956211725304 1101 18-19 0.34107635722534013 1101 20-21 0.3876906347523814 1101 22-23 0.01477828314592955 1101 24-25 0.2872677943973443 1101 26-27 0.4327711329516859 1101 28-29 0.3139313419933387 1101 30-31 0.36910005929528467 1101 32-33 0.322144792431331 1101 34-35 0.4637415479335303 1101 36-37 0.1950886172750259 1101 38-39 0.02376648899624456 1101 40-41 0.4801601745698463 1101 42-43 0.5880111027699755 1101 44-45 0.4605192431255887 1101 46-47 0.8073962471187031 1101 48-49 0.35769982140003975 1101 50-51 0.48301666446520386 1101 52-53 0.6728051975244185 1101 54-55 0.7844562746812969 1101 56-57 0.8666452633819901 1101 58-59 0.8505909786043588 1101 60-61 1.026007219644491 1101 62-63 0.7065782758511503 1101 64-65 0.6552628032004009 1101 66-67 0.9229700077719016 1101 68-69 0.8211708017440102 1101 70-71 0.8778594013002845 1101 72-73 0.5914540400788937 1101 74-75 0.6426688095591935 1101 76-77 0.9985271102961697 1101 78-79 0.7218788402129839 1101 80-81 0.8701847963913636 1101 82-83 0.6678382725598517 1101 84-85 0.596417445793886 1101 86-87 0.7223668080623824 1101 88-89 0.6444643038934608 1101 90-91 0.7595899616275474 1101 92-93 1.1195882705251528 1101 94-95 1.3085753534663525 1101 96-97 0.9659749556271429 1101 98-99 0.5990115731241303 1101 100-101 0.8709304124579553 1102 1 0.20099817175130497 1102 2 0.018893139376260137 1102 3 -0.19106358022053982 1102 4 0.172121123003123 1102 5 0.1589384733902932 1102 6 0.0071661802379026085 1102 7 -0.11144844178590319 1102 8 0.02736118032181878 1102 9 0.16038448858540733 1102 10-11 0.21958462927821643 1102 12-13 0.09359988441070755 1102 14-15 -0.110884895059435 1102 16-17 -0.1365323968132941 1102 18-19 -0.1469938887397575 1102 20-21 -0.22388885723737673 1102 22-23 -0.3805767433563574 1102 24-25 -0.1357527225462576 1102 26-27 -0.1629237333457354 1102 28-29 -0.32420813748971256 1102 30-31 -0.22649430673413917 1102 32-33 -0.2702856762721382 1102 34-35 -0.5049433130038778 1102 36-37 -0.12925180318831764 1102 38-39 -0.2761095606732269 1102 40-41 -0.5555620873561864 1102 42-43 -0.1993396357208823 1102 44-45 -0.17990382578037156 1102 46-47 -0.32906822789767176 1102 48-49 0.09249822530689045 1102 50-51 -0.21273180197514563 1102 52-53 -0.1374521576063117 1102 54-55 -0.43529872799870617 1102 56-57 -0.11768600039026467 1102 58-59 -0.3291556118309913 1102 60-61 -0.5693396179470724 1102 62-63 -0.44824052588787566 1102 64-65 -0.08852479134429814 1102 66-67 -0.6689207372700778 1102 68-69 -0.181700900358301 1102 70-71 -0.2000096952395296 1102 72-73 -0.026416024241278535 1102 74-75 -0.1246860978316704 1102 76-77 0.023775010957240994 1102 78-79 0.12683164230934452 1102 80-81 0.14413549914879553 1102 82-83 0.12534154812414044 1102 84-85 0.1403639796513083 1102 86-87 0.16908305486126451 1102 88-89 0.16261763008010988 1102 90-91 -0.05087211627132504 1102 92-93 0.1543595021373214 1102 94-95 -0.05579953486667932 1102 96-97 -0.7420866547593725 1102 98-99 -0.593721885276203 1102 100-101 -1.1008811234839158 1103 1 0.09446061191932387 1103 2 0.04536573877061656 1103 3 0.10727211383045088 1103 4 0.08432074203214057 1103 5 -0.09951126774480201 1103 6 -0.07072529187306742 1103 7 0.13462584755667706 1103 8 0.2812685750663704 1103 9 -0.04138971284079673 1103 10-11 -0.03771426199852357 1103 12-13 0.18440712774371804 1103 14-15 0.2651072168874222 1103 16-17 0.09843329137660106 1103 18-19 0.3290305813198344 1103 20-21 0.1768968954245338 1103 22-23 0.3388204188982087 1103 24-25 0.16837079997010562 1103 26-27 0.28881383625473944 1103 28-29 0.17320321349201606 1103 30-31 0.2912232863106965 1103 32-33 0.3994417829444217 1103 34-35 0.5011680965887777 1103 36-37 0.19095919612789203 1103 38-39 0.19374549940297214 1103 40-41 0.5636561894827352 1103 42-43 0.7230955259475706 1103 44-45 0.7392894193109711 1103 46-47 0.871881757449593 1103 48-49 0.7064964669792602 1103 50-51 0.6663282253766099 1103 52-53 1.0220519695793868 1103 54-55 0.6987218590873496 1103 56-57 1.0306454199477173 1103 58-59 1.2661237668932444 1103 60-61 1.110464645997446 1103 62-63 1.1149310575838385 1103 64-65 1.1416024431970726 1103 66-67 1.124213237527222 1103 68-69 1.2366742339583894 1103 70-71 1.2463613118914694 1103 72-73 1.2108818901867764 1103 74-75 1.2138621829146423 1103 76-77 1.1897599186450414 1103 78-79 1.0909629796151954 1103 80-81 0.8285187413095834 1103 82-83 1.1394264851174007 1103 84-85 1.609840510861627 1103 86-87 1.1017842856906093 1103 88-89 1.2576711125456725 1103 90-91 1.402987046569347 1103 92-93 1.0939139370303579 1103 94-95 1.4587463526003503 1103 96-97 1.2923655773398757 1103 98-99 0.7930674573046304 1103 100-101 1.33001293727607 1104 1 -0.06756550457786048 1104 2 -0.03564731947797739 1104 3 -0.2769699547225315 1104 4 -0.2574622675725067 1104 5 -0.013509665090268186 1104 6 -0.10193191015913072 1104 7 -0.2336235345184221 1104 8 -0.335222965852104 1104 9 -0.055192857203849144 1104 10-11 -0.060557732309909795 1104 12-13 -0.22120554902794964 1104 14-15 -0.2400449788621657 1104 16-17 -0.15662014925181467 1104 18-19 -0.32432765711453015 1104 20-21 -0.16855416153742198 1104 22-23 -0.24681437902059145 1104 24-25 -0.013835220142084381 1104 26-27 -0.08120749485143364 1104 28-29 0.1512005724979204 1104 30-31 0.05185780207201418 1104 32-33 -0.056451358371333527 1104 34-35 -0.24499173998560764 1104 36-37 -0.41239509210162595 1104 38-39 -0.44069690096721814 1104 40-41 -0.09034536802661108 1104 42-43 -0.05499889523454726 1104 44-45 -0.5032870171528074 1104 46-47 0.016566474671357412 1104 48-49 0.41926014315274784 1104 50-51 0.4439260493780566 1104 52-53 0.2977424089550311 1104 54-55 0.2712758358149969 1104 56-57 0.35740072168694326 1104 58-59 0.6314330679327114 1104 60-61 0.6347019743046118 1104 62-63 0.5162549405223373 1104 64-65 0.4765854573162329 1104 66-67 0.1671745368060229 1104 68-69 0.25667919993019694 1104 70-71 0.14406229267090254 1104 72-73 -0.2667091620349318 1104 74-75 0.015567542778303078 1104 76-77 0.3544608881381741 1104 78-79 0.6951016656642004 1104 80-81 0.5439629136288033 1104 82-83 0.4291006496490759 1104 84-85 0.3259449317051626 1104 86-87 0.5657979195404756 1104 88-89 0.5468994682446926 1104 90-91 0.5377003181290014 1104 92-93 0.2634962310836073 1104 94-95 0.1698452995886015 1104 96-97 0.4625516884196337 1104 98-99 0.3185857806119685 1104 100-101 0.5220323948565131 1105 1 0.21967633267084707 1105 2 0.3464793462728153 1105 3 0.2536203278254341 1105 4 0.465942962083588 1105 5 0.5066396228155838 1105 6 0.6091776744907733 1105 7 0.5401320179842131 1105 8 0.5877060079080252 1105 9 0.7674247184704583 1105 10-11 0.48503003157706814 1105 12-13 0.4703958614222046 1105 14-15 0.2608823463198675 1105 16-17 0.6799187526119894 1105 18-19 0.5419428928694359 1105 20-21 0.43451631517641687 1105 22-23 0.3913341761838751 1105 24-25 0.5273219900974198 1105 26-27 0.7400935080335742 1105 28-29 0.773493011935571 1105 30-31 0.36402293464517044 1105 32-33 0.5343120248772806 1105 34-35 0.02020036515704504 1105 36-37 -0.028677090544633188 1105 38-39 0.036749634729076774 1105 40-41 0.45665055632197493 1105 42-43 0.612801168876814 1105 44-45 -0.10124003267691961 1105 46-47 -0.10924064169078207 1105 48-49 -0.31267418848620565 1105 50-51 -0.4016685835843461 1105 52-53 0.19049324469254003 1105 54-55 -0.11166367052744874 1105 56-57 -0.00669462107993013 1105 58-59 -0.04898319803788809 1105 60-61 -0.14728502024592416 1105 62-63 -0.09407385922121847 1105 64-65 -0.08636961893050454 1105 66-67 -0.14665062232754877 1105 68-69 -0.14757733713991428 1105 70-71 -0.2229982009866518 1105 72-73 0.20526501024147592 1105 74-75 0.365615626306262 1105 76-77 0.19978075808367635 1105 78-79 0.24392934345877038 1105 80-81 -0.04731565027649509 1105 82-83 -0.18715845187585956 1105 84-85 -0.3326963651762753 1105 86-87 0.1511232847463191 1105 88-89 0.20212912433298413 1105 90-91 -0.085714070294312 1105 92-93 -0.056488199012104445 1105 94-95 -0.1711012590046117 1105 96-97 -0.39366711452948877 1105 98-99 0.13257121817207462 1105 100-101 0.9673660029528612 1106 1 0.28658930475623023 1106 2 0.18785865661764234 1106 3 0.3642935626366004 1106 4 0.12341422645140199 1106 5 -0.08047039360477726 1106 6 0.2651711063462727 1106 7 0.0899678143717324 1106 8 0.038937535001622336 1106 9 0.2079830107528906 1106 10-11 -0.07785995200256934 1106 12-13 -0.08469445056465474 1106 14-15 -0.48012750589688125 1106 16-17 -0.4229548106063987 1106 18-19 0.06747655460999624 1106 20-21 0.3572995664079386 1106 22-23 0.03386291181605827 1106 24-25 0.19965368139463635 1106 26-27 0.25474868044737065 1106 28-29 -0.3926810438936599 1106 30-31 0.08865347651708788 1106 32-33 -0.21252705558248408 1106 34-35 0.3443793470946446 1106 36-37 -0.34735525146417245 1106 38-39 -0.5436280335796297 1106 40-41 -0.05660888538574227 1106 42-43 -4.172219277762679E-4 1106 44-45 -0.399146436617805 1106 46-47 -0.17414211952328884 1106 48-49 -0.11636876976699995 1106 50-51 -0.572522442369241 1106 52-53 -0.4157464925816825 1106 54-55 -0.49130242257999157 1106 56-57 -0.22048772452819776 1106 58-59 0.28685424038575746 1106 60-61 0.06769855939283076 1106 62-63 0.04024469578700263 1106 64-65 0.23702939584782 1106 66-67 0.3584807405624275 1106 68-69 0.3341550109717488 1106 70-71 0.6602119796373174 1106 72-73 0.8518167343794119 1106 74-75 0.9411082371436983 1106 76-77 0.5815952080015769 1106 78-79 0.6230754846738762 1106 80-81 0.5651638242719486 1106 82-83 0.7661666712768529 1106 84-85 0.8318356545281596 1106 86-87 1.1070756656987015 1106 88-89 0.8305363492919327 1106 90-91 0.1987276374232536 1106 92-93 0.7761883198713129 1106 94-95 0.38899726316287797 1106 96-97 0.9295266457332367 1106 98-99 1.4921771295021244 1106 100-101 1.6738109947426842 1107 1 0.3347862534386188 1107 2 0.48591690904482476 1107 3 0.985514089682372 1107 4 0.15448218761645194 1107 5 -0.0021763575437532268 1107 6 -0.0676999061363901 1107 7 0.3172216285187517 1107 8 0.5046795599669451 1107 9 0.5738637319734181 1107 10-11 0.537535332324758 1107 12-13 0.26405728313853416 1107 14-15 0.10478927634999735 1107 16-17 0.6843267427916615 1107 18-19 0.42295505669044076 1107 20-21 0.6582357661305522 1107 22-23 0.7246396108451876 1107 24-25 0.5106106855555197 1107 26-27 0.8205253794764928 1107 28-29 0.5813827563837322 1107 30-31 0.5554357233964211 1107 32-33 0.7318904201456675 1107 34-35 0.5464251168588561 1107 36-37 0.5436641660115527 1107 38-39 0.6624052535216265 1107 40-41 0.5452288399956728 1107 42-43 0.46948569069357404 1107 44-45 0.28091597669703106 1107 46-47 0.7259619026681108 1107 48-49 0.13786146601663773 1107 50-51 0.07754690575836065 1107 52-53 0.38009261976228714 1107 54-55 0.3763120018305486 1107 56-57 0.004269557025196491 1107 58-59 -0.7616547748708342 1107 60-61 -0.85889644754198 1107 62-63 -0.585899548318416 1107 64-65 -0.7876585375779754 1107 66-67 -1.0993902719202353 1107 68-69 -0.6611639904152113 1107 70-71 -0.5629156209174653 1107 72-73 -0.4904162753293235 1107 74-75 -0.7763328169478392 1107 76-77 -0.7339040985171508 1107 78-79 -0.577826040984931 1107 80-81 -1.011465856726666 1107 82-83 -0.4778347156856988 1107 84-85 -0.2895929169004141 1107 86-87 0.1163336407333766 1107 88-89 0.45251970574130596 1107 90-91 0.10784691620272824 1107 92-93 0.27840745995453275 1107 94-95 0.04759643098534383 1107 96-97 0.1179108343601456 1107 98-99 0.7043823999598189 1107 100-101 0.9848026729673727 1108 1 -0.09328082511389724 1108 2 0.19529667314656507 1108 3 -0.0025305223693017354 1108 4 -0.16253618677173876 1108 5 0.4214776583432709 1108 6 0.2923257698645756 1108 7 0.23866911307302985 1108 8 0.30528936498981096 1108 9 0.18743991748252853 1108 10-11 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84-85 -2.27346388464235 2316 86-87 -3.015543381920345 2316 88-89 -2.8024129594638296 2316 90-91 -2.780189413267987 2316 92-93 -3.077344706242357 2316 94-95 -3.5113867614146983 2316 96-97 -3.37642573521914 2316 98-99 -4.035856664660706 2316 100-101 -4.312018498344873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 578.0 3 172.0 4 142.0 5 160.0 6 165.0 7 202.0 8 233.0 9 282.0 10 401.0 11 537.0 12 692.0 13 960.0 14 1132.0 15 1420.0 16 1835.0 17 2657.0 18 2327.0 19 1337.0 20 1319.0 21 1303.0 22 1324.0 23 1528.0 24 1679.0 25 1834.0 26 2056.0 27 2345.0 28 2831.0 29 3279.0 30 4159.0 31 5172.0 32 6615.0 33 8716.0 34 12644.0 35 18399.0 36 28374.0 37 44568.0 38 44906.0 39 9654.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37366541508689 19.368579533457574 15.331087221951915 21.92666782950362 2 14.619056361299798 20.204699834771432 42.250780246006975 22.92546355792179 3 18.429990270051956 25.893135797029608 31.03945218556663 24.6374217473518 4 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20.90081285144985 27.114090572253847 30.399221791668673 21.58587478462763 50-51 20.796892637220445 27.408527352317673 30.29609881890848 21.498481191553406 52-53 21.03364888242393 27.47800869098368 29.920799166693435 21.567543259898958 54-55 20.93810877601173 27.198592056684863 30.017461617712183 21.845837549591224 56-57 21.010111671213515 27.48047990858884 29.83757540158731 21.67183301861034 58-59 21.171539093366125 27.154046997389038 29.95039623360116 21.72401767564368 60-61 20.859765568538737 26.995409107907758 30.511422167057077 21.63340315649642 62-63 20.72491900474499 27.077425039235663 30.572381765283552 21.625274190735794 64-65 20.766712323424212 27.0673448645325 30.46315224349047 21.702790568552818 66-67 20.55265404279145 27.115248698908644 30.08412347288131 22.247973785418598 68-69 20.665973470319948 26.998620493362992 30.35511392980354 21.98029210651352 70-71 20.703563500462568 26.912313968196393 30.232937184781832 22.151185346559203 72-73 20.806394625871256 26.75065272354347 30.13540858887823 22.307544061707038 74-75 20.561592540244867 27.14623983553296 29.91450834266415 22.37765928155803 76-77 20.60827323189327 27.023504386276908 29.98226213942004 22.38596024240978 78-79 20.54981571833132 26.83788261969129 30.067745888659907 22.544555773317484 80-81 20.458088885317046 26.919782780909518 30.04672736248723 22.575400971286207 82-83 20.554040904751414 26.993142762723068 30.111487478886684 22.341328853638835 84-85 20.509761089644158 26.920782355694655 29.490009453617617 23.079447101043566 86-87 20.60621778306152 26.723936123823204 29.742172689675435 22.927673403439837 88-89 20.69362065482858 26.32742347172999 29.78703380648952 23.191922066951907 90-91 20.391562808418833 26.40661938534279 30.06977438086713 23.13204342537125 92-93 20.50196816507339 26.57585181954853 29.93079862744873 22.99138138792935 94-95 20.09972189843327 26.759933182197827 30.035428120382186 23.104916798986718 96-97 20.002891221242674 26.94687036773581 29.80183661387511 23.24840179714641 98-99 20.389000208868417 27.070494237756527 29.043726947009397 23.496778606365666 100-101 20.113116041535587 27.097331232978632 29.155280815280538 23.634271910205243 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 37.0 1 32.5 2 40.0 3 48.0 4 41.5 5 51.0 6 94.5 7 180.5 8 311.5 9 385.0 10 351.0 11 315.0 12 326.0 13 357.0 14 364.0 15 385.5 16 429.5 17 429.0 18 457.5 19 503.5 20 546.5 21 657.5 22 808.5 23 937.5 24 1102.0 25 1388.5 26 1739.5 27 2179.5 28 2601.0 29 2894.5 30 3299.5 31 3848.5 32 4240.0 33 4623.5 34 5180.0 35 5623.0 36 6147.0 37 6663.0 38 6818.0 39 6941.0 40 7183.5 41 7395.5 42 7435.5 43 7553.5 44 7865.5 45 8077.0 46 8150.0 47 8310.0 48 8752.5 49 8591.0 50 7775.0 51 7171.0 52 6603.0 53 6259.0 54 6005.5 55 5378.5 56 4718.5 57 4156.5 58 3640.0 59 3141.0 60 2622.5 61 2153.0 62 1665.5 63 1161.0 64 810.5 65 595.0 66 426.0 67 325.0 68 222.0 69 134.0 70 91.0 71 59.0 72 37.0 73 30.5 74 26.5 75 21.0 76 14.0 77 7.0 78 3.0 79 3.0 80 2.0 81 0.5 82 2.0 83 2.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036707687507456246 2 0.026613073442905782 3 0.024777689067532967 4 0.06469729923189164 5 0.11654690783617358 6 0.13765382815296093 7 0.0738742211087557 8 0.1059934476777799 9 0.07892152814103093 10-11 0.08763960392405179 12-13 0.08649248868944379 14-15 0.07341537501491249 16-17 0.057585184777321996 18-19 0.0738742211087557 20-21 0.06905633712340206 22-23 0.041754994539731485 24-25 0.011241729299158476 26-27 0.003441345703824023 28-29 0.0075709605484128506 30-31 0.006882691407648046 32-33 0.0075709605484128506 34-35 0.008488652736099257 36-37 0.00734153750149125 38-39 0.004129614844588828 40-41 0.008488652736099257 42-43 0.00298249960998082 44-45 0.0013765382815296092 46-47 0.0018353843753728125 48-49 6.882691407648046E-4 50-51 0.0018353843753728125 52-53 0.005047307032275235 54-55 0.014453651956060899 56-57 0.009865191017628866 58-59 0.005047307032275235 60-61 0.00298249960998082 62-63 0.011012306252236873 64-65 0.003441345703824023 66-67 0.02018922812910094 68-69 0.048867108994301134 70-71 0.060797107434224416 72-73 0.0022942304692160154 74-75 0.01009461406455047 76-77 0.01950095898833613 78-79 0.0298249960998082 80-81 0.08511595040791418 82-83 0.030283842193651402 84-85 0.0146830750029825 86-87 0.036248841413613044 88-89 0.05047307032275234 90-91 0.04267268672741789 92-93 0.043819801962025895 94-95 0.0146830750029825 96-97 0.016977305472198514 98-99 0.04473749414971231 100-101 0.09039268048711102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 217938.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.70492722474657 #Duplication Level Percentage of deduplicated Percentage of total 1 91.17775486242562 75.40849580412693 2 5.94905582111014 9.840324574817384 3 1.3517346878186653 3.353853569496247 4 0.534232196975347 1.7673453988785022 5 0.2797896927052612 1.156999308671142 6 0.196699887242164 0.976082991568742 7 0.11086165534818725 0.6418163596310735 8 0.08610962508233987 0.5697352220628106 9 0.05770104464230101 0.4294944628140022 >10 0.241098011047426 3.3492804503868463 >50 0.00942084315718207 0.5527382812581509 >100 0.0038791707117808516 0.5683212842123608 >500 5.541672445401217E-4 0.27407752254757406 >1k 0.0011083344890802434 1.1114347695282059 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1391 0.6382549165358955 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1034 0.47444686103387207 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 598 0.27438996411823546 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 260 0.1192999843992328 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2942304692160156E-4 0.0 16-17 0.0 0.0 0.0 4.588460938432031E-4 0.0 18-19 0.0 0.0 0.0 4.588460938432031E-4 0.0 20-21 0.0 0.0 0.0 4.588460938432031E-4 0.0 22-23 0.0 0.0 0.0 9.176921876864062E-4 0.0 24-25 0.0 0.0 0.0 0.0018353843753728125 0.0 26-27 0.0 0.0 0.0 0.0027530765630592183 0.0 28-29 0.0 0.0 0.0 0.005506153126118437 0.0 30-31 0.0 0.0 0.0 0.013535959768374493 0.0 32-33 0.0 0.0 0.0 0.02202461250447375 2.2942304692160156E-4 34-35 0.0 0.0 0.0 0.03097211133441621 9.176921876864062E-4 36-37 0.0 0.0 0.0 0.04014903321128027 9.176921876864062E-4 38-39 0.0 0.0 0.0 0.04726114766584992 9.176921876864062E-4 40-41 0.0 0.0 0.0 0.060338261340381213 9.176921876864062E-4 42-43 0.0 0.0 0.0 0.08259229689177655 9.176921876864062E-4 44-45 0.0 0.0 0.0 0.12021767658691922 9.176921876864062E-4 46-47 0.0 0.0 0.0 0.1608255558920427 9.176921876864062E-4 48-49 0.0 0.0 0.0 0.2514476594260753 9.176921876864062E-4 50-51 0.0 0.0 0.0 0.32050399654947737 9.176921876864062E-4 52-53 0.0 0.0 0.0 0.4090612926612156 9.176921876864062E-4 54-55 0.0 0.0 0.0 0.5669043489432775 9.176921876864062E-4 56-57 0.0 0.0 0.0 0.7362185575714194 9.176921876864062E-4 58-59 0.0 0.0 0.0 0.9486642990208225 9.176921876864062E-4 60-61 0.0 0.0 0.0 1.2076829189953107 9.176921876864062E-4 62-63 0.0 0.0 0.0 1.5882957538382474 9.176921876864062E-4 64-65 0.0 0.0 0.0 1.8317136066220667 9.176921876864062E-4 66-67 0.0 0.0 0.0 2.166212409033762 0.0011471152346080077 68-69 0.0 0.0 0.0 2.5816975470087824 0.0013765382815296094 70-71 0.0 0.0 0.0 2.8448457818278596 0.0013765382815296094 72-73 0.0 0.0 0.0 3.269278418632822 0.0013765382815296094 74-75 0.0 0.0 0.0 3.7799741210803073 0.0013765382815296094 76-77 0.0 0.0 0.0 4.157604456313264 0.0013765382815296094 78-79 0.0 0.0 0.0 4.47650249153429 0.0013765382815296094 80-81 0.0 0.0 0.0 4.907588396699978 0.0013765382815296094 82-83 0.0 0.0 0.0 5.368499297965476 0.0013765382815296094 84-85 0.0 0.0 0.0 5.9739467187915825 0.0013765382815296094 86-87 0.0 0.0 0.0 7.022868889317145 0.0013765382815296094 88-89 0.0 0.0 0.0 7.9832337637309685 0.0013765382815296094 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1375 0.0 37.672665 1 GGTATCA 850 0.0 32.986454 1 TATCAAC 1650 0.0 30.248764 2 ATCAACG 1655 0.0 30.157377 3 TCAACGC 1660 0.0 30.066542 4 AACGCAG 1655 0.0 29.870163 6 CAACGCA 1665 0.0 29.690763 5 CCCAATG 100 4.911081E-5 28.520262 2 CCAAGAC 100 4.911081E-5 28.520262 3 GTACATG 855 0.0 27.23532 1 ACGCAGA 1850 0.0 27.22931 7 GTGTAAG 105 6.858087E-5 27.155914 1 CCGGAAC 35 0.008375674 27.14968 64-65 CGCAGAG 1870 0.0 26.938086 8 TACATGG 915 0.0 25.974737 2 TGGGATA 130 9.91763E-6 25.595106 6 GCAGAGT 1925 0.0 25.18094 9 GTCTTAG 95 0.001219937 25.01203 1 ACATGGG 890 0.0 24.568016 3 GAGTACT 1120 0.0 23.973583 12-13 >>END_MODULE