FastQCFastQC Report
Thu 26 May 2016
SRR936940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217938
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.6717506813864493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.5363910837027044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.3739595664822105No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4130.1895034367572429No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.13765382815296093No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTT250.005015830557.07
CACCGAG500.001612942838.07
GGTATCA11450.034.0174671
GCCGAAC300.00394828431.66666888-89
GTATCAA16700.029.2964081
CATAGAG856.390398E-427.9411772
ACACCGT700.00837691627.1428556
GTAGGTT700.00837691627.1428553
GCGTAGA700.00837691627.1428551
TAAGGTG950.001223366225.05
GTACCTG1351.3362434E-524.629631
CAACGCA20400.024.2156875
TCAACGC20450.024.156484
ATCAACG20350.024.041773
TGGACAC1401.7709104E-523.7499985
AACGCAG21150.023.581566
GAGTACT13050.023.2950212-13
TATCAAC21200.023.0778312
GTGTACA2303.5852281E-922.7173921
ATAAGAC1050.002190554322.6190473
Produced by FastQC (version 0.11.5)