##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936940_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 217938 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.351141150235385 33.0 31.0 34.0 31.0 34.0 2 32.47172590369738 34.0 31.0 34.0 31.0 34.0 3 32.58680909249419 34.0 31.0 34.0 31.0 34.0 4 36.211160972386644 37.0 35.0 37.0 35.0 37.0 5 36.08323009296222 37.0 35.0 37.0 35.0 37.0 6 36.180074149528764 37.0 36.0 37.0 35.0 37.0 7 36.14781268067065 37.0 36.0 37.0 35.0 37.0 8 36.11439033119511 37.0 35.0 37.0 35.0 37.0 9 37.855032165111176 39.0 38.0 39.0 35.0 39.0 10-11 37.77277023740697 39.0 37.0 39.0 35.0 39.0 12-13 37.679576301516946 39.0 37.0 39.0 35.0 39.0 14-15 38.90634033532473 40.0 38.0 41.0 35.5 41.0 16-17 38.9189150125265 40.0 38.0 41.0 35.5 41.0 18-19 39.035819820315865 40.0 38.5 41.0 36.0 41.0 20-21 39.048752397470835 40.0 38.5 41.0 36.0 41.0 22-23 38.96188136075398 40.0 38.0 41.0 35.0 41.0 24-25 38.85591773807229 40.0 38.0 41.0 35.0 41.0 26-27 38.66746505886995 40.0 38.0 41.0 35.0 41.0 28-29 38.50550844735659 40.0 38.0 41.0 34.0 41.0 30-31 38.47611247235452 40.0 38.0 41.0 34.5 41.0 32-33 38.326395580394426 40.0 38.0 41.0 34.0 41.0 34-35 38.16277794602134 40.0 38.0 41.0 34.0 41.0 36-37 38.007456249024955 40.0 37.5 41.0 34.0 41.0 38-39 37.79931218970533 40.0 37.0 41.0 33.0 41.0 40-41 37.36960970551257 40.0 36.5 41.0 32.5 41.0 42-43 37.19968523157962 40.0 36.5 41.0 32.5 41.0 44-45 36.93153557433766 40.0 36.0 41.0 31.5 41.0 46-47 36.474917178280066 39.5 36.0 41.0 30.5 41.0 48-49 36.02423395644633 39.0 36.0 41.0 29.5 41.0 50-51 35.692332222925785 39.0 35.0 40.0 28.5 41.0 52-53 35.42154878910516 39.0 35.0 40.0 27.5 41.0 54-55 35.086451192541 38.5 35.0 40.0 26.0 41.0 56-57 34.838382934596076 38.0 34.5 40.0 26.0 41.0 58-59 34.51179693307271 38.0 34.0 40.0 25.5 41.0 60-61 34.10850104158064 38.0 34.0 40.0 24.5 41.0 62-63 33.738526553423455 37.0 33.5 40.0 23.0 41.0 64-65 33.442279455625 37.0 33.0 39.0 22.0 41.0 66-67 33.119699180500874 36.0 33.0 39.0 21.5 40.0 68-69 32.66468215731079 36.0 32.0 39.0 19.5 40.0 70-71 32.210688360909984 35.0 32.0 38.5 18.5 40.0 72-73 31.797288678431478 35.0 31.0 38.0 17.5 40.0 74-75 31.330006699152968 35.0 31.0 37.0 13.0 39.0 76-77 29.91205755765401 33.5 29.5 35.5 10.0 38.0 78-79 30.546263157411744 34.0 30.5 36.0 7.5 38.5 80-81 30.400203268819574 34.0 31.0 36.0 7.0 37.5 82-83 30.041580633024072 34.0 30.0 35.0 3.5 37.0 84-85 29.60879929154163 34.0 30.0 35.0 2.0 36.5 86-87 29.245769439014765 34.0 29.5 35.0 2.0 36.0 88-89 28.876368508474886 34.0 29.0 35.0 2.0 36.0 90-91 28.459910616780917 34.0 29.0 35.0 2.0 35.0 92-93 27.97728023566335 33.0 28.5 35.0 2.0 35.0 94-95 27.470429663482275 33.0 27.0 35.0 2.0 35.0 96-97 26.758683662325982 33.0 25.5 34.0 2.0 35.0 98-99 25.94627829933284 32.0 24.5 34.0 2.0 35.0 100-101 22.9709343941855 29.0 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10528713123126465 1101 2 0.164861396234258 1101 3 0.08019416458395057 1101 4 -0.040494930305882804 1101 5 0.0644969711761263 1101 6 -0.0844929017073568 1101 7 -0.05612053736185629 1101 8 0.007255494647807836 1101 9 0.0012305762242874607 1101 10-11 0.11290396988840001 1101 12-13 0.07535393094586595 1101 14-15 0.12370989375274632 1101 16-17 0.15751770222248496 1101 18-19 -0.030409756261803977 1101 20-21 -0.061360496666864606 1101 22-23 -0.007264056193491797 1101 24-25 0.13316675026555203 1101 26-27 0.05959241077862032 1101 28-29 0.17356047082267168 1101 30-31 0.11812045428484907 1101 32-33 0.20716686611051927 1101 34-35 0.05790470490286026 1101 36-37 -0.03690238701705795 1101 38-39 0.10459955914215158 1101 40-41 0.330762525025321 1101 42-43 0.17278120808172304 1101 44-45 -0.024608958092152022 1101 46-47 -0.14372717784141997 1101 48-49 -0.1893855323421434 1101 50-51 0.23934783139772975 1101 52-53 0.008285564322939365 1101 54-55 -0.3854470587677099 1101 56-57 -0.16116769755110738 1101 58-59 4.501632716724657E-4 1101 60-61 -0.08427728796745981 1101 62-63 -0.2076812358851967 1101 64-65 -0.11019417726597425 1101 66-67 0.1603073289295054 1101 68-69 -0.11948375363505193 1101 70-71 -0.18124212668434936 1101 72-73 -0.3992065924940391 1101 74-75 -0.22308033512301506 1101 76-77 -0.18985852007843818 1101 78-79 -0.23806406432782268 1101 80-81 -0.051379945297913565 1101 82-83 0.26022827861842757 1101 84-85 0.013136530292651827 1101 86-87 -0.5886096813685278 1101 88-89 -0.5826787477979707 1101 90-91 -0.3875226264770468 1101 92-93 -0.6674072056633875 1101 94-95 -0.5394733949545909 1101 96-97 -0.6962088670900819 1101 98-99 -0.3577277065893938 1101 100-101 -0.23633248230681048 1102 1 -0.07987158474753642 1102 2 -0.06414149221789245 1102 3 -0.04586294934613733 1102 4 0.0502121449129902 1102 5 -0.13649612036100933 1102 6 0.043000993831917356 1102 7 0.04679545930302709 1102 8 0.12435691802938464 1102 9 0.038668787170628605 1102 10-11 0.12487094624478345 1102 12-13 0.03417487669060648 1102 14-15 0.00701419367532452 1102 16-17 -0.024786061698669926 1102 18-19 0.15097275520267317 1102 20-21 -0.18683880349790627 1102 22-23 -0.17368319442229563 1102 24-25 -0.20378459271581306 1102 26-27 -0.07237746891883745 1102 28-29 -0.21456411973179712 1102 30-31 0.02845932629961112 1102 32-33 0.14415133695799653 1102 34-35 0.26530189685093575 1102 36-37 0.23789140132193154 1102 38-39 0.41571488878302887 1102 40-41 0.2645105150199569 1102 42-43 0.13284724048003227 1102 44-45 0.1808824509960445 1102 46-47 0.3863484579158296 1102 48-49 0.4701046707428489 1102 50-51 0.0822696347447689 1102 52-53 0.19279475079188302 1102 54-55 0.40184970735699466 1102 56-57 0.16081096661421412 1102 58-59 0.2563666512711933 1102 60-61 0.4889669351282393 1102 62-63 0.6713536637634263 1102 64-65 0.6631665516959586 1102 66-67 0.6205855285602624 1102 68-69 0.5462114104409466 1102 70-71 0.3232803853756856 1102 72-73 0.3184658212990641 1102 74-75 0.5570157729110505 1102 76-77 0.39038722168798756 1102 78-79 -0.04736666691254143 1102 80-81 0.17876008507549912 1102 82-83 0.38642683737013783 1102 84-85 -0.10999118693681353 1102 86-87 -0.16269326781364413 1102 88-89 -0.26183722555390787 1102 90-91 -0.735400581929369 1102 92-93 -0.550620310898335 1102 94-95 -0.6505392189079018 1102 96-97 -0.32705649379486346 1102 98-99 -0.5171025204777848 1102 100-101 -0.6574956054084709 1103 1 -0.23577663505523816 1103 2 0.11159969172271644 1103 3 0.2177763004340747 1103 4 0.08589764847848613 1103 5 0.181138440545503 1103 6 0.0800968226880272 1103 7 -0.04402094198641748 1103 8 -0.14190761259482088 1103 9 -0.0480631655498982 1103 10-11 0.10969926508819583 1103 12-13 0.2842786668635142 1103 14-15 0.23411319728966618 1103 16-17 0.10051309821145793 1103 18-19 -0.11785905587479562 1103 20-21 0.07304873679710511 1103 22-23 0.1597915784603856 1103 24-25 0.08099122051071816 1103 26-27 0.24758626557369467 1103 28-29 0.010306447817292508 1103 30-31 0.12033194523094437 1103 32-33 0.06927893781762151 1103 34-35 0.19140634639669685 1103 36-37 0.25496793741648816 1103 38-39 0.16268055255171276 1103 40-41 0.4676278833896035 1103 42-43 0.06738311524802754 1103 44-45 0.23441681164857897 1103 46-47 0.2165721562649523 1103 48-49 0.28093792267212336 1103 50-51 -0.06430651061031512 1103 52-53 0.04308963698900925 1103 54-55 0.08945349752990239 1103 56-57 -0.08386826069847331 1103 58-59 0.10304719732960876 1103 60-61 -0.1341434731945128 1103 62-63 0.47816962547095443 1103 64-65 0.21664901726204988 1103 66-67 0.33306538105611594 1103 68-69 0.31303152375539867 1103 70-71 0.2810499956130563 1103 72-73 0.4010766936249439 1103 74-75 0.274801134833492 1103 76-77 0.562657540140659 1103 78-79 0.4542451334195867 1103 80-81 0.11574418686313237 1103 82-83 0.23867847359169048 1103 84-85 0.21025033890083122 1103 86-87 0.015856356099611446 1103 88-89 0.3864239837847627 1103 90-91 -0.2017084602140251 1103 92-93 0.23436003002185757 1103 94-95 0.42443086510108685 1103 96-97 0.6763319500851814 1103 98-99 0.04553179621655801 1103 100-101 0.4564782150467437 1104 1 0.04789321811000491 1104 2 0.003544659726053112 1104 3 0.060693583144590946 1104 4 0.05466845759330141 1104 5 0.04428077206927128 1104 6 0.0014087421489961116 1104 7 0.18396230914503775 1104 8 -0.02611017909934077 1104 9 0.09587101275106136 1104 10-11 0.20813272670913108 1104 12-13 0.10086501269829995 1104 14-15 0.11649866956486932 1104 16-17 0.1036055442911632 1104 18-19 0.3014269728149799 1104 20-21 -0.05142786106075903 1104 22-23 0.0767074842423554 1104 24-25 0.026089016296417356 1104 26-27 -0.095985622511364 1104 28-29 0.39994336756041804 1104 30-31 0.3776144300226534 1104 32-33 0.40268186999824707 1104 34-35 0.24618440131583696 1104 36-37 0.26281755876114943 1104 38-39 0.17997680662888627 1104 40-41 0.46933775777308284 1104 42-43 0.27054215049514596 1104 44-45 0.3949700660530624 1104 46-47 -0.21778672548848022 1104 48-49 -0.07749802193471567 1104 50-51 -0.23851618748356174 1104 52-53 -0.07548986934824597 1104 54-55 -0.13380079408550927 1104 56-57 0.1487085143542899 1104 58-59 -0.2248593069648308 1104 60-61 -0.1718510000276865 1104 62-63 0.15447291091275872 1104 64-65 0.06054256488459231 1104 66-67 -0.4266436581412094 1104 68-69 -0.5172846054953055 1104 70-71 -0.1508146139374098 1104 72-73 -0.23187591520320439 1104 74-75 0.432363519854313 1104 76-77 0.5971936167972061 1104 78-79 0.4301714564560548 1104 80-81 0.8138018147086896 1104 82-83 0.7063883705477707 1104 84-85 0.5761521706672497 1104 86-87 0.4558583876335298 1104 88-89 0.08110136491181308 1104 90-91 0.41049428687128753 1104 92-93 0.3762626744018398 1104 94-95 0.3775080746763848 1104 96-97 -0.26412717658095985 1104 98-99 -0.12280900488498503 1104 100-101 0.42929516255416544 1105 1 0.08104795548235444 1105 2 0.2311171284717659 1105 3 0.0015508437573146239 1105 4 0.18742478859115153 1105 5 0.011492385386119963 1105 6 0.09759869498134321 1105 7 0.023088562751304664 1105 8 0.29389714791444277 1105 9 0.08895614349246728 1105 10-11 0.10547439452064111 1105 12-13 0.09954843990900031 1105 14-15 -0.18910649597984985 1105 16-17 -0.07004468238832118 1105 18-19 0.14989516899577637 1105 20-21 0.1051870585710617 1105 22-23 -0.1093872174107986 1105 24-25 -0.3129799950146577 1105 26-27 -0.39529413558550885 1105 28-29 -0.16858710823753853 1105 30-31 0.23535587802375346 1105 32-33 -0.053046938904074636 1105 34-35 0.10833350604633551 1105 36-37 0.20556381511503474 1105 38-39 0.14631833705889363 1105 40-41 0.4189645380084599 1105 42-43 0.26539034392830985 1105 44-45 0.5239140601914514 1105 46-47 0.024279492398591174 1105 48-49 -0.09886084649853188 1105 50-51 0.29203974968729796 1105 52-53 0.3583838312516505 1105 54-55 0.36736694873630427 1105 56-57 -0.015553975914521345 1105 58-59 -0.20448104987823257 1105 60-61 -0.36555892694072867 1105 62-63 -0.8070946120986449 1105 64-65 -0.5638449425569121 1105 66-67 0.00528380442233356 1105 68-69 -0.3812311182946857 1105 70-71 -0.07830007439443065 1105 72-73 -0.841735328126223 1105 74-75 -0.7256710086981464 1105 76-77 -0.005086916243048023 1105 78-79 -0.10771149449874784 1105 80-81 -0.4279209494072589 1105 82-83 -0.5503547718252619 1105 84-85 -0.7313992329138266 1105 86-87 -0.24387142873318268 1105 88-89 -0.040572857737817714 1105 90-91 0.03722872841545666 1105 92-93 -0.42382433388684504 1105 94-95 -1.094145540746979 1105 96-97 -0.7976024708063569 1105 98-99 0.31120207722336346 1105 100-101 0.9258377279248613 1106 1 -0.25105385075738695 1106 2 -0.1425281917252832 1106 3 0.23849665656519647 1106 4 -0.001819874791443965 1106 5 -0.10435326321815097 1106 6 -0.3666049174981296 1106 7 -0.34341390030288466 1106 8 -0.2524903726110139 1106 9 0.35825006795880654 1106 10-11 -0.14624974341039376 1106 12-13 0.0022167486117865565 1106 14-15 0.45091813456202345 1106 16-17 0.36062034224222117 1106 18-19 0.38930403599084684 1106 20-21 0.4612215413296781 1106 22-23 0.34820719966636915 1106 24-25 0.37281639250586807 1106 26-27 0.359919329110717 1106 28-29 0.3541550920908705 1106 30-31 0.48966622285134065 1106 32-33 0.10458852907621718 1106 34-35 0.3232760347819692 1106 36-37 0.4577805638835102 1106 38-39 0.10863360799485378 1106 40-41 0.25816815048793984 1106 42-43 0.7217942848150116 1106 44-45 0.742551170207868 1106 46-47 1.192998704221246 1106 48-49 1.0327893998068873 1106 50-51 1.1375652012472273 1106 52-53 1.2450422877376965 1106 54-55 1.0823915792781804 1106 56-57 1.3721718040526412 1106 58-59 1.0855846315667534 1106 60-61 1.1830863932563176 1106 62-63 1.2361319888865765 1106 64-65 0.9104571920900568 1106 66-67 0.9002345433385841 1106 68-69 0.6837114104409494 1106 70-71 0.9025291538485938 1106 72-73 0.5579362646488164 1106 74-75 0.3719480389209018 1106 76-77 0.5205288473037513 1106 78-79 0.427140722250023 1106 80-81 0.8084090998538223 1106 82-83 0.7518209260400894 1106 84-85 0.6026319657725416 1106 86-87 0.46791018046221566 1106 88-89 0.6732612966135783 1106 90-91 0.7504060683169342 1106 92-93 0.9618489009243234 1106 94-95 1.1816344264122947 1106 96-97 1.5907390727075992 1106 98-99 1.0620230952857632 1106 100-101 1.1640561187294587 1107 1 0.19866875673221074 1107 2 0.08833172505696751 1107 3 0.21464090067060226 1107 4 0.18854073547207406 1107 5 0.30889222915355674 1107 6 -0.14774222679078264 1107 7 0.20554587778310918 1107 8 0.18821697829051232 1107 9 0.040635643548263545 1107 10-11 0.24064346047310892 1107 12-13 0.11140537551886354 1107 14-15 0.39408734399336964 1107 16-17 0.3849268609662104 1107 18-19 0.21149543682302863 1107 20-21 0.19156134715492357 1107 22-23 0.1334707225512517 1107 24-25 0.3955625783588559 1107 26-27 0.4891495648943547 1107 28-29 0.4787736773890643 1107 30-31 0.5224332131426053 1107 32-33 0.19578131687094924 1107 34-35 0.20143137458779137 1107 36-37 0.2925239758113847 1107 38-39 0.6461509450267542 1107 40-41 0.479839440363115 1107 42-43 0.19675961075120796 1107 44-45 0.19802967228831392 1107 46-47 -0.49112057455822367 1107 48-49 -0.7530289080987984 1107 50-51 -0.7786275865475005 1107 52-53 -0.6499299730112682 1107 54-55 -0.4232256190574688 1107 56-57 -0.451163840884945 1107 58-59 -0.5910450494318553 1107 60-61 -1.3829676982832098 1107 62-63 -1.7363714785198034 1107 64-65 -1.3716509909890036 1107 66-67 -0.8649873706697342 1107 68-69 -1.387647812860024 1107 70-71 -1.4780880444870554 1107 72-73 -1.763284262396951 1107 74-75 -1.0234056365298585 1107 76-77 -1.4349496547766947 1107 78-79 -1.128719194532227 1107 80-81 -1.5713759209506115 1107 82-83 -1.607714441504985 1107 84-85 -1.9082210161969435 1107 86-87 -1.0352451593436065 1107 88-89 -0.5722657491561876 1107 90-91 -0.8375620315443726 1107 92-93 -1.2064936788260248 1107 94-95 -1.1020687636015722 1107 96-97 -2.197055656834703 1107 98-99 -2.1858964608862195 1107 100-101 -2.6274487356394722 1108 1 -0.4272640042402678 1108 2 -0.15061555890356004 1108 3 -0.16103108841709712 1108 4 -0.06468882402403153 1108 5 -0.14372752531969013 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-0.2943678828166192 2316 74-75 1.3544291475753312E-4 2316 76-77 0.047564177564886734 2316 78-79 -0.047552058087919846 2316 80-81 -0.30268921043803587 2316 82-83 -0.5768657099046095 2316 84-85 -0.2907244090354446 2316 86-87 -0.5544016474947746 2316 88-89 -1.4435205774263586 2316 90-91 -1.0747475415140961 2316 92-93 -0.9269759838683029 2316 94-95 -0.5046942986261058 2316 96-97 -0.9803054741433961 2316 98-99 -1.3892057833624705 2316 100-101 -2.2206213006253783 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 8.0 7 21.0 8 49.0 9 102.0 10 205.0 11 327.0 12 526.0 13 794.0 14 1030.0 15 1414.0 16 1978.0 17 2992.0 18 2810.0 19 1568.0 20 1210.0 21 1224.0 22 1209.0 23 1281.0 24 1549.0 25 1675.0 26 1971.0 27 2339.0 28 2836.0 29 3520.0 30 4462.0 31 5673.0 32 7489.0 33 9991.0 34 14164.0 35 21149.0 36 33649.0 37 51171.0 38 35397.0 39 2155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3790289640233 20.307860352194446 15.22687949479327 21.08623118898899 2 15.435619530952458 21.56508252612065 40.68214251299723 22.317155429929656 3 19.5258262708948 26.280989460256865 30.74787667995797 23.44530758889037 4 12.716919490864372 18.324018757628316 38.116345015554884 30.842716735952425 5 12.997632288970873 38.48539911532038 34.13450066993374 14.382467925775014 6 30.214556433481082 37.828189668621356 17.557286934816325 14.399966963081242 7 26.644274977287118 34.66536354376015 21.19960722774367 17.490754251209058 8 25.837164698216924 35.31325422826676 19.80746817902339 19.04211289449293 9 26.71034881480054 17.26408428085052 20.630638071378097 35.39492883297085 10-11 24.20459029632281 27.926061540438106 27.473409868861786 20.3959382943773 12-13 25.985030654576157 25.340274973383753 28.070046624325418 20.604647747714676 14-15 22.62834514151463 26.454379428067988 27.420065342681987 23.497210087735397 16-17 20.96743110425901 29.823160715432827 27.66291330561903 21.54649487468913 18-19 20.781827859299433 27.67140195835513 31.467435692719945 20.079334489625488 20-21 21.895519306215157 26.632941014522675 31.537614426319777 19.93392525294239 22-23 21.401121907012495 26.93632202631549 31.30992394434056 20.352632122331457 24-25 21.40177803269286 26.928821336392318 31.45901921422426 20.210381416690566 26-27 21.292801835388588 27.084829366217377 31.46934327502151 20.153025523372527 28-29 20.860519964393543 27.404124108691462 31.39333204856427 20.342023878350723 30-31 21.035799172241646 27.280006240306875 31.25017206728519 20.434022520166288 32-33 21.22805568556195 27.275417779368443 31.002165753562938 20.494360781506668 34-35 21.237232607438813 27.253163743817048 31.249024952050586 20.260578696693553 36-37 21.624765409552477 26.95232020850445 31.20362680273295 20.219287579210125 38-39 21.16082224909311 27.353159151869193 31.16107464165992 20.324943957377776 40-41 21.19649256436493 27.550851851681905 30.762100974134725 20.490554609818435 42-43 21.293441253934606 27.622534849360825 30.521753893309107 20.56227000339546 44-45 21.477946659019214 27.58680814453685 30.305248064238597 20.629997132205332 46-47 21.825523372526526 27.86831086894178 29.616518497275596 20.689647261256095 48-49 21.783259459112227 28.178197469004946 29.212207141480604 20.826335930402223 50-51 21.56913130163654 28.45048513395387 29.09174756056431 20.888636003845278 52-53 21.722232928631076 28.72399489763144 28.706558746065397 20.84721342767209 54-55 21.50290449577403 28.540456460094155 28.726518551147574 21.23012049298424 56-57 21.805104674505305 28.770863206194434 28.64330369945512 20.78072841984514 58-59 21.834650221622663 28.224770347530033 28.80612834842937 21.134451082417936 60-61 21.548217469010183 28.400046803053208 29.119758459537152 20.93197726839945 62-63 21.439124888729822 28.377336673732895 29.24249098367426 20.94104745386303 64-65 21.29825913791996 28.526461654231937 29.129155998494983 21.04612320935312 66-67 21.163128963283135 28.47759454523764 28.912810065247914 21.446466426231314 68-69 21.394846240673953 28.37228936670062 28.929328524626268 21.30353586799916 70-71 21.52658445655291 28.434987094924004 28.875709779179807 21.162718669343274 72-73 21.460969314597076 28.1656438199025 28.687811872669915 21.685574992830514 74-75 21.200858083536957 28.666184855055004 28.338782393227106 21.794174668180933 76-77 21.04199359450853 28.587258761666163 28.518890693683524 21.851856950141784 78-79 21.26522221916325 28.47759454523764 28.227523424093093 22.029659811506026 80-81 21.306270031913403 28.461434336621032 28.230629985752536 22.00166564571303 82-83 21.356581588758246 28.94728993404072 28.06859764840837 21.627530828792658 84-85 21.254516880226674 28.762575568123154 27.76662498709463 22.216282564555538 86-87 21.120275308287926 28.515744192715804 27.617780326928592 22.74620017206768 88-89 21.09887992070885 28.50466432035828 27.90149175656515 22.49496400236772 90-91 21.183102339912494 28.356096184421535 27.74008823711603 22.72071323854994 92-93 20.991749947232698 28.279602455744296 27.848516550578605 22.8801310464444 94-95 21.018363020675604 28.958924097679155 27.513100055979223 22.509612825666014 96-97 21.190430305866805 29.105984270755904 27.284135855151465 22.419449568225826 98-99 21.091875174935875 29.34150694925598 26.449157325282073 23.11746055052607 100-101 20.718943151731512 29.62634962442241 26.22917345398314 23.425533769862938 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 107.0 1 74.5 2 48.5 3 62.5 4 65.5 5 68.0 6 92.5 7 174.5 8 297.0 9 364.5 10 357.5 11 334.5 12 364.0 13 405.0 14 430.0 15 483.5 16 505.0 17 497.0 18 535.0 19 587.5 20 613.0 21 702.0 22 827.0 23 937.0 24 1070.0 25 1287.0 26 1658.0 27 2063.5 28 2424.0 29 2831.5 30 3392.0 31 3822.0 32 4214.5 33 4735.5 34 5121.5 35 5421.5 36 5934.5 37 6441.0 38 6713.5 39 6887.5 40 7066.0 41 7206.0 42 7192.0 43 7450.0 44 7863.0 45 7839.0 46 7829.5 47 8218.0 48 8723.5 49 8410.0 50 7624.5 51 7199.5 52 6753.5 53 6366.0 54 6019.0 55 5529.0 56 4891.5 57 4356.5 58 3928.5 59 3462.5 60 2867.5 61 2279.0 62 1750.0 63 1237.5 64 881.0 65 625.5 66 435.0 67 362.0 68 261.5 69 140.0 70 94.0 71 59.5 72 36.0 73 25.0 74 20.0 75 15.0 76 11.0 77 5.0 78 3.0 79 2.0 80 1.0 81 0.5 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021565766410630547 2 0.0032119226569024216 3 4.588460938432031E-4 4 0.0 5 0.0027530765630592183 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.01192999843992328 14-15 0.004588460938432031 16-17 0.0 18-19 0.0 20-21 0.0013765382815296092 22-23 0.0025236535161376173 24-25 2.2942304692160156E-4 26-27 2.2942304692160156E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0022942304692160154 38-39 0.010782883205315274 40-41 4.588460938432031E-4 42-43 0.0 44-45 2.2942304692160156E-4 46-47 2.2942304692160156E-4 48-49 0.0 50-51 0.0039001917976672264 52-53 0.0 54-55 0.0 56-57 2.2942304692160156E-4 58-59 0.0 60-61 0.0016059613284512108 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 2.2942304692160156E-4 72-73 2.2942304692160156E-4 74-75 0.004817883985353632 76-77 0.0 78-79 0.0 80-81 0.002064807422294414 82-83 2.2942304692160156E-4 84-85 0.0025236535161376173 86-87 2.2942304692160156E-4 88-89 0.0032119226569024216 90-91 0.0011471152346080077 92-93 0.0 94-95 0.0 96-97 0.0 98-99 4.588460938432031E-4 100-101 0.0032119226569024216 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 217938.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.05790477667797 #Duplication Level Percentage of deduplicated Percentage of total 1 89.57147351472183 70.8133302383361 2 6.810484829205079 10.7684532222061 3 1.6756523356505515 3.9742068837203806 4 0.7317630062041105 2.314066002543207 5 0.3506116594820548 1.3859311594462664 6 0.1960221794745027 0.9298261679407267 7 0.14560656477988512 0.8057944953259144 8 0.10171177563145332 0.6432895898030616 9 0.06677628115446597 0.47512735891654007 >10 0.3243684009592142 4.407022564698652 >50 0.015665800543072234 0.8357617702737793 >100 0.008123007689000417 1.0655733218144838 >500 5.802148349286012E-4 0.3738451387338805 >1k 0.0011604296698572024 1.2077720862408678 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1464 0.6717506813864493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1169 0.5363910837027044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 815 0.3739595664822105 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 413 0.1895034367572429 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.13765382815296093 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.2942304692160156E-4 14-15 0.0 0.0 0.0 6.882691407648047E-4 4.588460938432031E-4 16-17 0.0 0.0 0.0 9.176921876864062E-4 4.588460938432031E-4 18-19 0.0 0.0 0.0 9.176921876864062E-4 4.588460938432031E-4 20-21 0.0 0.0 0.0 9.176921876864062E-4 4.588460938432031E-4 22-23 0.0 0.0 0.0 0.0013765382815296094 4.588460938432031E-4 24-25 0.0 0.0 0.0 0.0022942304692160154 4.588460938432031E-4 26-27 0.0 0.0 0.0 0.003211922656902422 4.588460938432031E-4 28-29 0.0 0.0 0.0 0.005964999219961641 4.588460938432031E-4 30-31 0.0 0.0 0.0 0.013765382815296094 4.588460938432031E-4 32-33 0.0 0.0 0.0 0.02179518945755215 4.588460938432031E-4 34-35 0.0 0.0 0.0 0.03143095742825941 4.588460938432031E-4 36-37 0.0 0.0 0.0 0.040607879305123475 4.588460938432031E-4 38-39 0.0 0.0 0.0 0.04771999375969312 4.588460938432031E-4 40-41 0.0 0.0 0.0 0.06056768438730281 4.588460938432031E-4 42-43 0.0 0.0 0.0 0.08282171993869816 4.588460938432031E-4 44-45 0.0 0.0 0.0 0.12159421486844882 4.588460938432031E-4 46-47 0.0 0.0 0.0 0.1626609402674155 4.588460938432031E-4 48-49 0.0 0.0 0.0 0.25374188989529134 4.588460938432031E-4 50-51 0.0 0.0 0.0 0.3211922656902422 4.588460938432031E-4 52-53 0.0 0.0 0.0 0.40814360047352916 4.588460938432031E-4 54-55 0.0 0.0 0.0 0.5652983876148262 4.588460938432031E-4 56-57 0.0 0.0 0.0 0.7343831731960466 4.588460938432031E-4 58-59 0.0 0.0 0.0 0.9479760298800577 4.588460938432031E-4 60-61 0.0 0.0 0.0 1.2099771494645264 4.588460938432031E-4 62-63 0.0 0.0 0.0 1.5917370995420717 4.588460938432031E-4 64-65 0.0 0.0 0.0 1.835613798419734 4.588460938432031E-4 66-67 0.0 0.0 0.0 2.170571446925272 4.588460938432031E-4 68-69 0.0 0.0 0.0 2.5874331231818224 4.588460938432031E-4 70-71 0.0 0.0 0.0 2.851269627141664 4.588460938432031E-4 72-73 0.0 0.0 0.0 3.2796024557442944 4.588460938432031E-4 74-75 0.0 0.0 0.0 3.796263157411741 4.588460938432031E-4 76-77 0.0 0.0 0.0 4.1782525305362075 4.588460938432031E-4 78-79 0.0 0.0 0.0 4.501968449742588 4.588460938432031E-4 80-81 0.0 0.0 0.0 4.9344308931898055 6.882691407648047E-4 82-83 0.0 0.0 0.0 5.394194679220696 9.176921876864062E-4 84-85 0.0 0.0 0.0 6.002624599656784 9.176921876864062E-4 86-87 0.0 0.0 0.0 7.061870807293817 9.176921876864062E-4 88-89 0.0 0.0 0.0 8.024071066083014 9.176921876864062E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCGTT 25 0.0050158305 57.0 7 CACCGAG 50 0.0016129428 38.0 7 GGTATCA 1145 0.0 34.017467 1 GCCGAAC 30 0.003948284 31.666668 88-89 GTATCAA 1670 0.0 29.296408 1 CATAGAG 85 6.390398E-4 27.941177 2 ACACCGT 70 0.008376916 27.142855 6 GTAGGTT 70 0.008376916 27.142855 3 GCGTAGA 70 0.008376916 27.142855 1 TAAGGTG 95 0.0012233662 25.0 5 GTACCTG 135 1.3362434E-5 24.62963 1 CAACGCA 2040 0.0 24.215687 5 TCAACGC 2045 0.0 24.15648 4 ATCAACG 2035 0.0 24.04177 3 TGGACAC 140 1.7709104E-5 23.749998 5 AACGCAG 2115 0.0 23.58156 6 GAGTACT 1305 0.0 23.29502 12-13 TATCAAC 2120 0.0 23.077831 2 GTGTACA 230 3.5852281E-9 22.717392 1 ATAAGAC 105 0.0021905543 22.619047 3 >>END_MODULE