FastQCFastQC Report
Thu 26 May 2016
SRR936939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077998
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35690.3310766810328034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27000.2504642865756708No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18160.16846042385978452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16480.15287597936174277No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12210.11326551626255336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60300.028.3701761
GTACATG49400.024.7219681
GGTATCA44500.024.026991
TACATGG50300.023.8083672
ATCAACG71850.023.6773513
TCAACGC72150.023.57894
TATCAAC72500.023.4661622
CAACGCA72550.023.377975
AACGCAG73550.022.930936
CGCAGTA1050.002191344722.6275429
ACATGGG50300.022.579113
GAGTACT30350.022.54291312-13
ACGCAGA81650.020.5979047
CGCAGAG81250.020.5257998
AGAGTAC59600.020.2494810-11
GTACACG1656.301752E-520.1600191
GTACTTT34000.019.9840514-15
CATGGGA29550.019.4582944
GCAGAGT84650.019.2541289
AGTACTT34850.018.95039612-13
Produced by FastQC (version 0.11.5)