FastQCFastQC Report
Thu 26 May 2016
SRR936939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077998
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.4322828057194911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37720.3499078848012705No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25270.2344160193247112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22820.21168870443173363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG753.0749207E-431.6726381
TCGGCGA350.00839115327.14419634-35
GTACATG45700.025.9895821
GTATCAA77650.025.635871
TACATGG48250.023.82282
GGTATCA57900.023.3031851
ACATGGG47800.023.0533923
GAGTACT44450.022.7584312-13
GTACTTT47100.021.4794614-15
ATCAACG92000.021.3224433
TCAACGC92550.021.1947464
TATCAAC93050.021.1328812
CAACGCA92900.021.1148955
CATGGGG21600.021.1092174
AACGCAG94500.020.7564326
AGTACTT47900.020.52434312-13
AGAGTAC73600.019.93953910-11
TGCGTAA1603.9835868E-1019.29514360-61
ACTTTTT54050.018.84978116-17
ACGCAGA103250.018.7214187
Produced by FastQC (version 0.11.5)