##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936939_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1077998 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35709342688948 33.0 31.0 34.0 31.0 34.0 2 32.368462650209 33.0 31.0 34.0 31.0 34.0 3 32.547321052543694 34.0 31.0 34.0 31.0 34.0 4 36.22347722351989 37.0 35.0 37.0 35.0 37.0 5 36.16920439555547 37.0 35.0 37.0 35.0 37.0 6 36.22297258436472 37.0 36.0 37.0 35.0 37.0 7 36.177120922302265 37.0 36.0 37.0 35.0 37.0 8 36.15710140464083 37.0 36.0 37.0 35.0 37.0 9 37.88958050014935 39.0 38.0 39.0 35.0 39.0 10-11 37.817660607904656 39.0 38.0 39.0 35.0 39.0 12-13 37.73942298594246 39.0 37.0 39.0 35.0 39.0 14-15 39.0152509559387 40.0 38.0 41.0 36.0 41.0 16-17 38.94024478709608 40.0 38.0 41.0 36.0 41.0 18-19 38.98846890253971 40.0 38.5 41.0 36.0 41.0 20-21 39.053493141916775 40.0 38.0 41.0 36.0 41.0 22-23 38.98901389427439 40.0 38.0 41.0 35.5 41.0 24-25 38.88546592850822 40.0 38.0 41.0 35.0 41.0 26-27 38.74122679262856 40.0 38.0 41.0 35.0 41.0 28-29 38.573095682923345 40.0 38.0 41.0 34.0 41.0 30-31 38.46080651355568 40.0 38.0 41.0 34.5 41.0 32-33 38.46983250432747 40.0 38.0 41.0 34.0 41.0 34-35 38.39102669949295 40.0 38.0 41.0 34.0 41.0 36-37 38.238692001283866 40.0 38.0 41.0 34.0 41.0 38-39 38.096588769181395 40.0 38.0 41.0 34.0 41.0 40-41 37.92793075682886 40.0 37.5 41.0 33.0 41.0 42-43 37.71338675953017 40.0 37.0 41.0 33.0 41.0 44-45 37.331527516748636 40.0 37.0 41.0 32.5 41.0 46-47 37.06802888317047 40.0 37.0 41.0 32.0 41.0 48-49 36.73426156634799 40.0 36.5 41.0 31.0 41.0 50-51 36.47732602472361 39.0 36.0 41.0 30.5 41.0 52-53 36.23565628136602 39.0 36.0 41.0 30.0 41.0 54-55 36.000835808600755 39.0 35.0 40.0 30.0 41.0 56-57 35.72844615667191 39.0 35.0 40.0 29.0 41.0 58-59 35.40053042770024 38.0 35.0 40.0 28.0 41.0 60-61 35.058058085450995 38.0 34.0 40.0 28.0 41.0 62-63 34.69613997428567 37.5 34.0 40.0 27.0 41.0 64-65 34.29584470472116 37.0 34.0 39.5 26.0 41.0 66-67 33.91840893953421 36.0 33.5 39.0 26.0 40.5 68-69 33.47914977578808 36.0 33.0 39.0 26.0 40.0 70-71 33.002218928049956 35.0 32.5 38.0 25.0 40.0 72-73 32.66544093773829 35.0 32.0 37.5 24.5 39.5 74-75 32.23243781528352 35.0 32.0 37.0 23.5 39.0 76-77 30.854723756444816 34.0 30.5 35.5 21.5 38.0 78-79 31.563311805773296 34.5 31.5 36.0 23.0 38.0 80-81 31.38674607930627 35.0 31.5 36.0 22.0 37.0 82-83 31.072300690724845 34.0 31.0 35.0 20.5 37.0 84-85 30.716819047901758 34.0 31.0 35.0 19.5 36.5 86-87 30.383620377774356 34.0 31.0 35.0 18.0 36.0 88-89 30.056936098211686 34.0 30.5 35.0 16.0 36.0 90-91 29.664268857641666 34.0 30.0 35.0 8.5 35.0 92-93 29.257787584021493 34.0 30.0 35.0 4.0 35.0 94-95 28.818029810815975 34.0 29.0 35.0 2.0 35.0 96-97 28.22674114423218 34.0 29.0 35.0 2.0 35.0 98-99 27.58043985239305 33.0 28.5 35.0 2.0 35.0 100-101 24.64886808695378 31.0 21.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15174341116947687 1101 2 0.07651584762028563 1101 3 0.1382830405764821 1101 4 0.10654136199803332 1101 5 0.16577115560123445 1101 6 0.10291957497000936 1101 7 0.2200569717115357 1101 8 0.2022687503701377 1101 9 0.2568992565194179 1101 10-11 0.2138838305926356 1101 12-13 0.2435364163942566 1101 14-15 0.3356108084529197 1101 16-17 0.28281960837955467 1101 18-19 0.29260744519005044 1101 20-21 0.28779422517121134 1101 22-23 0.22150453857387475 1101 24-25 0.23453898870813106 1101 26-27 0.27655886238132155 1101 28-29 0.2254332101148293 1101 30-31 0.45183604271202427 1101 32-33 0.41393881601035076 1101 34-35 0.4020053451132597 1101 36-37 0.482951266204374 1101 38-39 0.552991783700115 1101 40-41 0.4973019273619741 1101 42-43 0.6638157203245925 1101 44-45 0.6589907588810817 1101 46-47 0.6630814368045677 1101 48-49 0.47201212806429993 1101 50-51 0.4631264371950863 1101 52-53 0.3139157571883473 1101 54-55 0.34814115417446345 1101 56-57 0.551036920087185 1101 58-59 0.7201851949956151 1101 60-61 0.6765063303243934 1101 62-63 0.7172162888999551 1101 64-65 0.6647397708665039 1101 66-67 0.5155480532983319 1101 68-69 0.5212792037372012 1101 70-71 0.3649445062810699 1101 72-73 0.3670833706951484 1101 74-75 0.23050215887818837 1101 76-77 0.1800333881738574 1101 78-79 0.3070598471133579 1101 80-81 0.560616520676934 1101 82-83 0.7151867521478543 1101 84-85 0.8093964413744636 1101 86-87 0.5368815452629185 1101 88-89 0.6948076385931259 1101 90-91 0.9101085090071201 1101 92-93 0.8506377321842571 1101 94-95 0.8108536429960864 1101 96-97 0.6309691721275676 1101 98-99 0.8320420942721931 1101 100-101 1.1817787170185419 1102 1 0.11578511598573726 1102 2 0.01804681878505221 1102 3 0.07122799083121834 1102 4 0.10026142188500842 1102 5 0.09074948123262772 1102 6 0.1054039122564987 1102 7 0.1140407251630009 1102 8 0.13619501160232517 1102 9 0.2483924607158272 1102 10-11 0.16982004177796028 1102 12-13 0.20701116654257845 1102 14-15 0.3533576824372844 1102 16-17 0.23535881805510428 1102 18-19 0.23382354157099172 1102 20-21 0.2001242175457918 1102 22-23 0.19345817568147083 1102 24-25 0.14511027374836516 1102 26-27 0.240830139949189 1102 28-29 0.14400578076246973 1102 30-31 0.16523561903776596 1102 32-33 0.22706254492003808 1102 34-35 0.3267547732070213 1102 36-37 0.36850069681894126 1102 38-39 0.3377923119875348 1102 40-41 0.3023278150657873 1102 42-43 0.304848563632639 1102 44-45 0.39315676608877226 1102 46-47 0.34688863530576697 1102 48-49 0.24798265812528086 1102 50-51 0.3544073091033937 1102 52-53 0.3980118572424587 1102 54-55 0.11459274268285924 1102 56-57 0.2727220471039331 1102 58-59 0.1905860650313329 1102 60-61 0.17721080357195973 1102 62-63 0.3854838879020761 1102 64-65 0.34139847901111153 1102 66-67 0.3940766089976364 1102 68-69 0.25257397605054877 1102 70-71 0.18906409477866504 1102 72-73 0.25872953336409665 1102 74-75 0.12998980651371994 1102 76-77 0.14170126572425445 1102 78-79 0.31777076640368307 1102 80-81 0.26887816879321846 1102 82-83 0.44237194713614514 1102 84-85 0.3506833001800196 1102 86-87 0.18458797843159047 1102 88-89 0.30343921393935247 1102 90-91 0.4068787354918939 1102 92-93 0.3464219211937518 1102 94-95 0.3668288815491856 1102 96-97 0.5572574128710599 1102 98-99 0.7807645444166411 1102 100-101 0.7673240513349846 1103 1 -0.0965230828470851 1103 2 -0.37990103083487625 1103 3 -0.11521124605249611 1103 4 0.01762998765910595 1103 5 -0.045089704274964504 1103 6 0.038133757078902875 1103 7 0.13884688885021745 1103 8 0.19858443535763115 1103 9 0.03906100152546799 1103 10-11 0.08404950851554105 1103 12-13 0.12955940401602817 1103 14-15 0.0955897577792797 1103 16-17 0.11794584088130478 1103 18-19 0.20983957670936348 1103 20-21 0.13095821117080675 1103 22-23 0.031236719276996894 1103 24-25 0.19265941573097933 1103 26-27 0.20734068833500174 1103 28-29 0.08146032917722579 1103 30-31 0.14686865605389698 1103 32-33 0.25254256793075314 1103 34-35 0.08367426827382474 1103 36-37 0.23234764663315133 1103 38-39 0.1978041429673354 1103 40-41 0.1765259676962856 1103 42-43 0.1902734839453828 1103 44-45 0.3466677088405774 1103 46-47 0.42675498503242437 1103 48-49 0.32571888878235455 1103 50-51 0.19123114328154145 1103 52-53 0.32836193846738126 1103 54-55 0.1669855312122337 1103 56-57 0.18351844932830375 1103 58-59 0.21212016683564627 1103 60-61 0.3051653827400358 1103 62-63 0.28982345149450595 1103 64-65 0.008047507749175509 1103 66-67 0.10273285210075755 1103 68-69 0.31210288959032084 1103 70-71 0.35195036541504265 1103 72-73 0.5042635193912446 1103 74-75 0.3841957741033468 1103 76-77 0.2650098335968103 1103 78-79 0.32792071593206984 1103 80-81 0.33718986626746883 1103 82-83 0.2826374386645121 1103 84-85 0.33491725328302735 1103 86-87 0.2918021625824174 1103 88-89 0.4871291952341039 1103 90-91 0.6111806715032984 1103 92-93 0.47846714303366866 1103 94-95 0.38324707954985726 1103 96-97 0.6864538831145168 1103 98-99 0.5115306094038168 1103 100-101 0.558373390500595 1104 1 0.2125243797730505 1104 2 0.11458338671749857 1104 3 0.0821618319710069 1104 4 0.16690835237633195 1104 5 0.18876039480418427 1104 6 0.19083114711164484 1104 7 0.17480414176166192 1104 8 0.24864960506378253 1104 9 0.15882649655031855 1104 10-11 0.21633473989448504 1104 12-13 0.15199871141570753 1104 14-15 0.25722917326530137 1104 16-17 0.3412556672013807 1104 18-19 0.30699701188404305 1104 20-21 0.303678596042964 1104 22-23 0.2556295283562591 1104 24-25 0.16967941170423018 1104 26-27 0.20981660733381347 1104 28-29 0.18337064930353364 1104 30-31 0.11658434658786376 1104 32-33 0.1820416619128835 1104 34-35 0.15776003796514715 1104 36-37 0.14932726917869132 1104 38-39 0.12527290919353362 1104 40-41 0.1467238080887867 1104 42-43 0.07744035018323814 1104 44-45 0.12102104521162715 1104 46-47 0.25425236234912774 1104 48-49 0.329825110105034 1104 50-51 0.04073680721779027 1104 52-53 0.04735069536848613 1104 54-55 -0.09449016525588405 1104 56-57 0.008802175975823445 1104 58-59 0.05782188032207358 1104 60-61 0.2032118810604544 1104 62-63 0.21215626205729166 1104 64-65 -1.0003010753223407E-4 1104 66-67 0.03961970053615005 1104 68-69 0.058003291205260155 1104 70-71 0.27707116779718177 1104 72-73 0.26255835138056227 1104 74-75 0.11404191001662412 1104 76-77 0.08321311009650856 1104 78-79 0.21989027161243158 1104 80-81 0.31928382720803583 1104 82-83 0.26927182071793965 1104 84-85 0.35253930077744045 1104 86-87 0.3570312102569737 1104 88-89 0.4761899567129788 1104 90-91 0.3932730429388336 1104 92-93 0.2614030118041306 1104 94-95 0.2636572248306166 1104 96-97 0.33969488386687985 1104 98-99 0.5227215195543948 1104 100-101 0.4565099607726104 1105 1 0.05075556191917485 1105 2 -0.11996448424579143 1105 3 -0.03895593387809981 1105 4 -0.020488302610978337 1105 5 0.0776914751155573 1105 6 0.020225144039343945 1105 7 0.020360521967745626 1105 8 0.048190485675483785 1105 9 0.006968443837891414 1105 10-11 0.09076040915592642 1105 12-13 0.07871911461227654 1105 14-15 0.1620689129825763 1105 16-17 0.12585790756591564 1105 18-19 0.17921511085361885 1105 20-21 0.0724774637786112 1105 22-23 0.18565006790783656 1105 24-25 0.13704051082914503 1105 26-27 0.08568867526967949 1105 28-29 0.07073968808703768 1105 30-31 0.1318716414080967 1105 32-33 0.02475184711405376 1105 34-35 0.1737722581353296 1105 36-37 0.05970578377213087 1105 38-39 0.040422122994726806 1105 40-41 -0.07756982119820321 1105 42-43 -0.005473849723557578 1105 44-45 0.12423833295521547 1105 46-47 -0.15844936538962173 1105 48-49 -0.09678482827630575 1105 50-51 -0.21396571448681811 1105 52-53 -0.17978222487246853 1105 54-55 -0.18063028296995753 1105 56-57 -0.21964251394598477 1105 58-59 -0.30897136061204833 1105 60-61 -0.2179921854255511 1105 62-63 -0.3503720014012899 1105 64-65 -0.2399812804311594 1105 66-67 -0.20039445276829326 1105 68-69 -0.18929290984070946 1105 70-71 -0.3724964660119596 1105 72-73 -0.34842944570937817 1105 74-75 -0.40230515662472754 1105 76-77 -0.37463420681669035 1105 78-79 -0.2700401700742283 1105 80-81 0.0467912922131255 1105 82-83 -0.010104053076009478 1105 84-85 0.19271224725378389 1105 86-87 0.14841150264352976 1105 88-89 0.1403337680943082 1105 90-91 0.20204636568595546 1105 92-93 0.12617163979660617 1105 94-95 0.20004847739806308 1105 96-97 0.5460345286534611 1105 98-99 0.3182666806776844 1105 100-101 0.4121911316996538 1106 1 0.3483657616538167 1106 2 0.22862812114994568 1106 3 0.20536695604670996 1106 4 0.1965641245537455 1106 5 -0.00649192083078276 1106 6 0.24948837792052103 1106 7 0.2062728502056146 1106 8 0.27426630248287864 1106 9 0.2861038251841279 1106 10-11 0.3544629982333021 1106 12-13 0.3326983023547072 1106 14-15 0.5287255819029753 1106 16-17 0.651743089380858 1106 18-19 0.4419701169285233 1106 20-21 0.3950496636047518 1106 22-23 0.5668504074923035 1106 24-25 0.5399661225132846 1106 26-27 0.4566603036992021 1106 28-29 0.41804512601374455 1106 30-31 0.35666588270624544 1106 32-33 0.5059811632173705 1106 34-35 0.5241608993399396 1106 36-37 0.3860139210903242 1106 38-39 0.37827460709576854 1106 40-41 0.4831578452769847 1106 42-43 0.41734795485270126 1106 44-45 0.403205955508092 1106 46-47 0.08052659426693509 1106 48-49 0.20658915691007707 1106 50-51 0.17319158734174778 1106 52-53 0.3039156872930917 1106 54-55 0.3470799111727274 1106 56-57 0.3292612077574333 1106 58-59 0.19189081791380858 1106 60-61 0.327754620802871 1106 62-63 0.32633443596113665 1106 64-65 0.29332646544985863 1106 66-67 0.2554483826603189 1106 68-69 0.34103991343811657 1106 70-71 0.38459439316724087 1106 72-73 0.37325007399211074 1106 74-75 0.45658885540306215 1106 76-77 0.3937051336370736 1106 78-79 0.39547268889145926 1106 80-81 0.36418482892434056 1106 82-83 0.607887823331513 1106 84-85 0.7046754826600399 1106 86-87 0.6536450686362798 1106 88-89 0.9215676594374429 1106 90-91 1.1710908014317951 1106 92-93 1.210898472561425 1106 94-95 0.8328966658572732 1106 96-97 1.249668794149546 1106 98-99 1.3965678432969604 1106 100-101 1.473433766834443 1107 1 0.1705263481207524 1107 2 0.0699304667371834 1107 3 0.031803084729077113 1107 4 0.008599226544944827 1107 5 0.10265946936328874 1107 6 -0.002387577589530565 1107 7 0.011907495889630582 1107 8 0.036464104166917366 1107 9 0.02878935192438803 1107 10-11 0.13269583645463712 1107 12-13 -0.012346019285764953 1107 14-15 0.08022669695689899 1107 16-17 0.10900929038979257 1107 18-19 0.19668104445702994 1107 20-21 0.03647976117780871 1107 22-23 0.12606105684173485 1107 24-25 0.20189205675082178 1107 26-27 0.07251721212452367 1107 28-29 0.04425251669405128 1107 30-31 0.1820132559010048 1107 32-33 0.237517319483878 1107 34-35 0.09860126849992668 1107 36-37 0.016513801870729594 1107 38-39 0.2559751648565012 1107 40-41 0.16050343251534116 1107 42-43 0.09086916425374625 1107 44-45 0.39945930417633946 1107 46-47 0.1732893799848867 1107 48-49 0.2455254609153883 1107 50-51 0.38482858936615116 1107 52-53 0.34740863463542837 1107 54-55 0.02055261455902979 1107 56-57 0.06304325094765062 1107 58-59 0.11481943576121267 1107 60-61 0.18941955761648188 1107 62-63 0.16435239838124716 1107 64-65 0.3384656952444729 1107 66-67 0.1518933008663268 1107 68-69 0.08197863923769688 1107 70-71 0.3682016978224212 1107 72-73 0.278645228533442 1107 74-75 0.3328213757054641 1107 76-77 0.39335565656697824 1107 78-79 0.4296331958786652 1107 80-81 0.5587745966097515 1107 82-83 0.6469310743331924 1107 84-85 0.46053769752649387 1107 86-87 0.3663860208035068 1107 88-89 0.4971330174936526 1107 90-91 0.7920408414054272 1107 92-93 0.7776895016369814 1107 94-95 0.9430304489523991 1107 96-97 0.7951177611467521 1107 98-99 0.7230092316676711 1107 100-101 0.7560420813497828 1108 1 0.06522073259506556 1108 2 -0.12648525394903487 1108 3 0.013260707126178772 1108 4 0.09438746620880067 1108 5 0.15880311910677136 1108 6 0.08757922604117852 1108 7 0.1523072451757983 1108 8 0.21367584128881134 1108 9 0.1354065722507798 1108 10-11 0.10397401274361329 1108 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-1.4119534400661848 2316 58-59 -1.1071504037007074 2316 60-61 -0.8021307720630304 2316 62-63 -0.5469838398936275 2316 64-65 -0.4580529900779098 2316 66-67 -0.8029895436078363 2316 68-69 -0.9921648558179754 2316 70-71 -0.9879227130909598 2316 72-73 -0.8314939404582233 2316 74-75 -0.7138588403887454 2316 76-77 -1.0406257155825038 2316 78-79 -0.8623060267534051 2316 80-81 -0.6643851716915563 2316 82-83 -0.610831981358789 2316 84-85 -0.5992691795916976 2316 86-87 -0.5160635519889674 2316 88-89 -0.9905793236314722 2316 90-91 -0.8594510759604823 2316 92-93 -1.2255445969429424 2316 94-95 -0.7399072863774698 2316 96-97 -0.7618590620175425 2316 98-99 -0.7824771544666334 2316 100-101 -1.1650529666653462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 11.0 6 34.0 7 93.0 8 237.0 9 417.0 10 718.0 11 1181.0 12 1962.0 13 2804.0 14 3519.0 15 4994.0 16 7131.0 17 10521.0 18 9367.0 19 5386.0 20 4526.0 21 4352.0 22 4538.0 23 4927.0 24 5661.0 25 6742.0 26 8107.0 27 10320.0 28 12821.0 29 16239.0 30 20865.0 31 27012.0 32 35567.0 33 48524.0 34 69294.0 35 103527.0 36 167792.0 37 262392.0 38 199801.0 39 16608.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81633736120183 19.36633439225699 14.83164227183659 21.98568597470459 2 15.761062661124164 20.92900285999742 39.58355172161084 23.726382757267576 3 19.804391224971425 24.99628931471064 29.783073337984057 25.416246122333874 4 13.467093630971485 17.170996606672738 37.01082933363512 32.35108042872065 5 14.089129668453959 37.25588600901686 33.68698862687619 14.96799569565298 6 31.67574988752267 36.50211735675954 17.274940978390436 14.547191777327354 7 28.619467401115216 32.91150160900263 20.66091093017884 17.80812005970332 8 26.631774827040495 34.354330898573096 19.561168017009308 19.4527262573771 9 26.851626811923584 17.042703233215644 20.226568138345343 35.87910181651543 10-11 25.04850433187863 26.747628586920275 27.099021193482535 21.10484588771856 12-13 26.35815141313796 24.08981151048571 27.772727230561294 21.77930984581504 14-15 23.264607169957642 25.37917510051039 26.310577570644845 25.04564015888712 16-17 22.294475499954544 28.739292651748887 26.705569027029735 22.26066282126683 18-19 22.123278521852548 27.212341766867844 29.35775391048963 21.306625800789984 20-21 23.06063172620312 26.04388631562933 29.46022466364926 21.43525729451829 22-23 22.79888274375277 25.990168812929156 29.364525722682234 21.846422720635843 24-25 22.731360861113213 26.185127651610202 29.49496430040813 21.58854718686845 26-27 22.610260182768958 26.187576126674788 29.54809628812595 21.654067402430304 28-29 22.08861513970686 26.48203285206644 29.695204848817692 21.73414715940901 30-31 22.415142410076633 26.189889689424167 29.43101379125745 21.96395410924175 32-33 22.478852935542328 26.375098389451075 29.349810967020456 21.796237707986137 34-35 22.779966835581014 26.190243167086052 29.191321590500596 21.838468406832334 36-37 22.811552605115608 25.971792911770052 29.31817254136532 21.898481941749015 38-39 22.511231728438467 26.30937880633374 29.38714606024455 21.792243404983243 40-41 22.584454679969756 26.39573447624879 29.16326899795444 21.856541845827014 42-43 22.500173938857014 26.473215735204757 28.810211834336 22.21639849160223 44-45 22.801314111766292 26.35143816202462 28.665883419195254 22.181364307013837 46-47 23.028193054519683 26.819858329715508 28.169213985951707 21.9827346298131 48-49 23.103143431225035 26.969723393636702 27.896769369773857 22.03036380536441 50-51 22.833882733167936 27.016974576400898 27.903713794458135 22.24542889597303 52-53 23.119206480624683 27.200812629147503 27.461020934940965 22.21895995528684 54-55 22.763473428853708 27.00868774670557 27.682370785422393 22.545468039018328 56-57 22.858436267818437 27.311810900178713 27.585978182573793 22.243774649429053 58-59 23.15882483905082 27.01079797398839 27.518738752110426 22.311638434850366 60-61 22.9330147135901 26.966473497262943 28.119228225305964 21.98128356384099 62-63 22.83436518435099 26.942721600596663 28.09694452123288 22.12596869381947 64-65 22.688231392538928 27.139736269648747 27.818171698384504 22.35386063942783 66-67 22.63272654410288 27.215394484119383 27.638679717420235 22.513199254357506 68-69 22.5711098587891 27.058179633997298 27.8622167491112 22.508493758102407 70-71 22.831053405188985 27.12499924628558 27.589751153646887 22.454196194878545 72-73 22.768177677509605 27.01772173974349 27.711878871760433 22.50222171098648 74-75 22.735197819277797 27.547341514288377 27.283332011430517 22.43412865500331 76-77 22.889365600250468 27.258201045930498 27.275501803128517 22.576931550690524 78-79 22.88321909325942 27.18431381279841 27.283665107287337 22.648801986654828 80-81 22.817546092632416 27.543328094936815 27.12442645124302 22.51469936118775 82-83 22.894540934295936 27.451407844783404 27.132523638016565 22.521527582904096 84-85 22.794740421000096 27.350209702242296 27.120052098553092 22.73499777820451 86-87 22.690547808279856 27.347741488110838 27.148850509403026 22.81286019420628 88-89 22.733483798969747 27.27528254051301 26.965028335221113 23.026205325296132 90-91 22.70465076368629 27.16603509292715 27.220348888445677 22.908965254940885 92-93 22.87889216863111 27.113269226844576 27.134141250725886 22.87369735379843 94-95 22.67891904828828 27.067763332744278 27.181214746457428 23.072102872510015 96-97 22.51228689268241 27.159274122477456 27.225648389855824 23.102790594984313 98-99 22.514530777975835 27.299170060776245 26.934364159316047 23.25193500193187 100-101 22.22907654901859 27.664011413845596 26.650596620188143 23.456315416947668 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 246.0 1 194.0 2 152.0 3 168.0 4 151.0 5 145.5 6 255.5 7 531.0 8 883.5 9 1122.0 10 1121.0 11 1089.0 12 1158.5 13 1282.5 14 1405.0 15 1503.5 16 1598.5 17 1693.0 18 1846.5 19 1913.5 20 1979.5 21 2174.5 22 2443.0 23 2861.5 24 3335.5 25 4003.5 26 5080.5 27 6371.5 28 7682.0 29 9192.5 30 11020.5 31 13212.5 32 15197.5 33 17181.0 34 19710.5 35 22191.0 36 25127.0 37 27446.0 38 29212.5 39 30602.5 40 31137.5 41 32970.5 42 34585.5 43 35788.0 44 37909.5 45 39327.0 46 40106.0 47 42289.5 48 46682.0 49 45883.0 50 41438.5 51 39440.0 52 38068.0 53 37117.0 54 35773.5 55 33903.0 56 31428.5 57 28328.0 58 25808.5 59 23300.5 60 19785.0 61 15792.5 62 12222.0 63 9466.5 64 7106.5 65 5298.5 66 3921.5 67 3024.0 68 2086.0 69 1256.5 70 833.0 71 523.5 72 349.0 73 239.5 74 166.5 75 113.5 76 73.5 77 36.5 78 19.5 79 12.0 80 9.5 81 5.5 82 2.5 83 3.0 84 1.5 85 1.0 86 1.0 87 0.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01614103180154323 2 0.0023191137645895447 3 0.0027829365175074537 4 0.0 5 0.0016697619105044722 6 2.782936517507454E-4 7 9.276455058358179E-5 8 0.0 9 0.0 10-11 1.391468258753727E-4 12-13 6.493518540850725E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0028757010680910355 22-23 0.0 24-25 0.0041280225009693895 26-27 0.0023191137645895447 28-29 0.0041280225009693895 30-31 0.016465707728585768 32-33 0.0010667923317111906 34-35 0.0032931415457171536 36-37 0.0038961111245104352 38-39 0.0023191137645895447 40-41 0.004916521180929835 42-43 0.0030612301692581993 44-45 6.957341293768634E-4 46-47 1.8552910116716357E-4 48-49 3.7105820233432715E-4 50-51 3.2467592704253623E-4 52-53 0.0014378505340455177 54-55 0.0039888756750940165 56-57 6.029695787932816E-4 58-59 0.0016697619105044722 60-61 0.0013914682587537269 62-63 0.0 64-65 2.782936517507454E-4 66-67 1.391468258753727E-4 68-69 4.638227529179089E-5 70-71 3.2467592704253623E-4 72-73 0.0 74-75 6.029695787932816E-4 76-77 9.740277811276088E-4 78-79 4.638227529179089E-4 80-81 0.0055658730350149075 82-83 0.0019016732869634268 84-85 0.0038033465739268535 86-87 0.0019016732869634268 88-89 0.0010204100564193998 90-91 2.782936517507454E-4 92-93 0.0 94-95 3.2467592704253623E-4 96-97 0.0018552910116716357 98-99 0.002551025141048499 100-101 8.348809552522361E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1077998.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.657742151790906 #Duplication Level Percentage of deduplicated Percentage of total 1 79.50394986283706 46.63522191103209 2 11.52079322907892 13.515674372308197 3 3.5687666342553284 6.280073790961913 4 1.6784863507549086 3.9382487827152763 5 0.9187717732655997 2.694653888627862 6 0.6026887645559879 2.1211417289463936 7 0.38979402557765164 1.6005106211249738 8 0.2784521973002479 1.306670175267004 9 0.22322230661268253 1.1784344853433266 >10 1.2042909239330262 12.709748740032268 >50 0.07821430112868691 3.0721813763582433 >100 0.030345364392469158 3.0612103442132947 >500 0.0012710093185529761 0.4583240154710537 >1k 9.532569889147321E-4 1.427905767598106 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4660 0.4322828057194911 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3772 0.3499078848012705 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2527 0.2344160193247112 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2282 0.21168870443173363 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.391468258753727E-4 0.0 24-25 0.0 0.0 0.0 3.7105820233432715E-4 0.0 26-27 0.0 0.0 0.0 5.565873035014907E-4 0.0 28-29 4.638227529179089E-5 0.0 0.0 0.0018552910116716357 0.0 30-31 9.276455058358179E-5 0.0 0.0 0.005009285731513416 0.0 32-33 9.276455058358179E-5 0.0 0.0 0.007606693147853706 0.0 34-35 9.276455058358179E-5 0.0 0.0 0.010946216968862651 0.0 36-37 9.276455058358179E-5 0.0 0.0 0.013729153486370105 0.0 38-39 9.276455058358179E-5 0.0 0.0 0.017022295032087256 0.0 40-41 9.276455058358179E-5 0.0 0.0 0.02319113764589545 0.0 42-43 1.391468258753727E-4 0.0 0.0 0.030055714389080498 0.0 44-45 1.8552910116716357E-4 0.0 0.0 0.045918452538872986 0.0 46-47 2.782936517507454E-4 0.0 0.0 0.0779686047655005 0.0 48-49 2.782936517507454E-4 0.0 0.0 0.10287588659719221 0.0 50-51 2.782936517507454E-4 0.0 0.0 0.13061248722168317 0.0 52-53 2.782936517507454E-4 0.0 0.0 0.15983332065551142 0.0 54-55 2.782936517507454E-4 0.0 0.0 0.19893357872649114 0.0 56-57 2.782936517507454E-4 0.0 0.0 0.2410023024161455 0.0 58-59 2.782936517507454E-4 0.0 0.0 0.29911929335675946 0.0 60-61 2.782936517507454E-4 0.0 0.0 0.3724032883177891 0.0 62-63 2.782936517507454E-4 0.0 0.0 0.4665129248848328 0.0 64-65 2.782936517507454E-4 0.0 0.0 0.5365037783001453 0.0 66-67 2.782936517507454E-4 0.0 0.0 0.6459659479887718 0.0 68-69 2.782936517507454E-4 0.0 0.0 0.7827008955489714 0.0 70-71 3.7105820233432715E-4 0.0 0.0 0.8769496789418905 0.0 72-73 4.1744047762611806E-4 0.0 0.0 1.045178191425216 9.276455058358179E-5 74-75 4.638227529179089E-4 0.0 0.0 1.17606897229865 9.276455058358179E-5 76-77 4.638227529179089E-4 0.0 0.0 1.2849281724084831 9.276455058358179E-5 78-79 4.638227529179089E-4 0.0 0.0 1.4224516186486431 9.276455058358179E-5 80-81 5.102050282096999E-4 0.0 0.0 1.5404017447156675 9.276455058358179E-5 82-83 6.493518540850725E-4 0.0 0.0 1.7002814476464705 1.8552910116716357E-4 84-85 6.493518540850725E-4 0.0 0.0 1.9661910318943079 1.8552910116716357E-4 86-87 6.493518540850725E-4 0.0 0.0 2.3141972434086147 1.8552910116716357E-4 88-89 6.493518540850725E-4 0.0 0.0 2.7494485147467804 1.8552910116716357E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTAG 75 3.0749207E-4 31.672638 1 TCGGCGA 35 0.008391153 27.144196 34-35 GTACATG 4570 0.0 25.989582 1 GTATCAA 7765 0.0 25.63587 1 TACATGG 4825 0.0 23.8228 2 GGTATCA 5790 0.0 23.303185 1 ACATGGG 4780 0.0 23.053392 3 GAGTACT 4445 0.0 22.75843 12-13 GTACTTT 4710 0.0 21.47946 14-15 ATCAACG 9200 0.0 21.322443 3 TCAACGC 9255 0.0 21.194746 4 TATCAAC 9305 0.0 21.132881 2 CAACGCA 9290 0.0 21.114895 5 CATGGGG 2160 0.0 21.109217 4 AACGCAG 9450 0.0 20.756432 6 AGTACTT 4790 0.0 20.524343 12-13 AGAGTAC 7360 0.0 19.939539 10-11 TGCGTAA 160 3.9835868E-10 19.295143 60-61 ACTTTTT 5405 0.0 18.849781 16-17 ACGCAGA 10325 0.0 18.721418 7 >>END_MODULE