FastQCFastQC Report
Thu 26 May 2016
SRR936938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857233
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30070.3507797763268563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23100.26947166056369737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.1644827019025166No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13210.15410046043491094No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10600.12365366242316848No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8950.10440568666861869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51250.026.4233361
GTACATG38450.025.7041721
TACATGG39650.024.9276962
ACATGGG38950.024.1557063
GGTATCA36800.024.145181
GGTATAG1000.001650136123.7578241
GAGTACT24800.022.6082512-13
TATCAAC60300.022.1437422
ATCAACG60200.022.1015913
TCAACGC60300.022.064944
CAACGCA60200.022.0252295
AACGCAG61850.021.5900676
CATGGGG18600.021.2044584
TATAGAC2551.1854354E-820.4981443
GTACTTT27450.020.33852614-15
TGCGCAA600.004758579719.79587676-77
ACGCAGA67550.019.7682557
CATGGGA22250.019.4345024
CGCAGAG67150.019.3917628
AGAGTAC48750.019.05721910-11
Produced by FastQC (version 0.11.5)