FastQCFastQC Report
Thu 26 May 2016
SRR936938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857233
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40180.468717373222916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30510.3559125698614029No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21450.2502236848091476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.22770938589624992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC702.0530459E-433.9281543
ATCCGCG600.003954020331.6607367
GCACCGT1951.6370905E-1129.2252966
GTATCAA66200.023.8298021
GGTATCA50600.023.4763091
TTGCGTA1550.022.98224860-61
GCGTAAG1453.6379788E-1222.92941762-63
GAGTACT35900.022.75671812-13
TCACGCA1302.942068E-421.9215284
GTACATG39900.021.9121651
GATCCGC1100.002879904421.5868666
ACATGGG39250.021.1780833
GTACTTT38400.020.96898714-15
TGCGTAA1701.8189894E-1220.95440360-61
TACATGG41900.020.860152
TGCACCG1404.852663E-420.3557055
TCAACGC77600.020.2595234
ATCAACG77500.020.2255573
CGTAAGT1652.7284841E-1120.15009162-63
CAACGCA78350.020.0655885
Produced by FastQC (version 0.11.5)