FastQCFastQC Report
Thu 26 May 2016
SRR936937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901295
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.33418581041723294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.25607597956274025No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14190.15744012781608685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13030.14456975795938068No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10850.1203823387459156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT702.0496297E-433.938016
GTATCAA47150.029.828021
TAGTTCG700.00838378827.1473927
GGTATCA32750.026.114131
GTACATG40600.025.2779621
TACATGG40500.024.6378482
ATCAACG56200.024.6034013
TCAACGC56500.024.4727634
CAACGCA56550.024.4511265
TATCAAC56800.024.4271622
TGTACCG1000.001650161923.7579235
AACGCAG58300.023.7158556
ACATGGG40600.023.4068223
CATGGGG18700.021.5981124
ACGCAGA64500.021.139197
GAGTACT26200.021.02996312-13
CGCAGAG64500.020.9918798
GTATATA4350.020.7528971
AGAGTAC50800.020.33768310-11
AGTACTT29100.020.15838612-13
Produced by FastQC (version 0.11.5)