FastQCFastQC Report
Thu 26 May 2016
SRR936937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901295
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40770.45234911987750953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32380.3592608413449537No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21990.24398226995600772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19840.22012770513538854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT905.689453E-736.949998
CGTCGAT458.946033E-426.39065444-45
GTATCAA68700.025.8626881
ATACGTC1501.0583008E-625.3371375
GTATAGG3500.024.4322381
GAGTACT37300.022.72707712-13
GGTATCA50050.022.3060441
TCAACGC80350.021.8690574
TATCAAC81250.021.7449252
ATCAACG81350.021.6002313
CAACGCA81750.021.5016985
AGTACTT39300.021.4496412-13
AACGCAG82750.021.241866
GTACTTT40050.021.10725214-15
TTGCGTA1552.6011548E-1019.92068760-61
GTACATG41150.019.8571721
GCGTCGA600.00476268719.79299244-45
AGAGTAC65500.019.54682710-11
ACGCAGA90950.019.1177677
GTACTCG1250.006024927419.0028539
Produced by FastQC (version 0.11.5)