##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936937_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 901295 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48928375282233 33.0 31.0 34.0 31.0 34.0 2 32.61835359122152 34.0 31.0 34.0 31.0 34.0 3 32.724124731636145 34.0 31.0 34.0 31.0 34.0 4 36.28951675089732 37.0 35.0 37.0 35.0 37.0 5 36.22492080839237 37.0 35.0 37.0 35.0 37.0 6 36.27426314358784 37.0 37.0 37.0 35.0 37.0 7 36.234666785014895 37.0 37.0 37.0 35.0 37.0 8 36.21040502832036 37.0 37.0 37.0 35.0 37.0 9 37.95261373911982 39.0 38.0 39.0 35.0 39.0 10-11 37.872967785242345 39.0 38.0 39.0 35.0 39.0 12-13 37.79586927698478 39.0 38.0 39.0 35.0 39.0 14-15 39.08930982641644 40.0 38.0 41.0 36.0 41.0 16-17 39.00974819565181 40.0 38.0 41.0 36.0 41.0 18-19 39.14987268319474 40.0 38.5 41.0 36.0 41.0 20-21 39.164696353580126 40.0 39.0 41.0 36.0 41.0 22-23 39.084833489589975 40.0 38.5 41.0 36.0 41.0 24-25 38.97852257030162 40.0 38.0 41.0 35.0 41.0 26-27 38.81550990519197 40.0 38.0 41.0 35.0 41.0 28-29 38.75035809585097 40.0 38.0 41.0 35.0 41.0 30-31 38.77706966087685 40.0 38.0 41.0 35.0 41.0 32-33 38.629656771645244 40.0 38.0 41.0 35.0 41.0 34-35 38.487713789602736 40.0 38.0 41.0 35.0 41.0 36-37 38.34896121691566 40.0 38.0 41.0 34.5 41.0 38-39 38.20210308500546 40.0 38.0 41.0 34.0 41.0 40-41 38.03879417948619 40.0 38.0 41.0 34.0 41.0 42-43 37.80593534858176 40.0 37.5 41.0 33.0 41.0 44-45 37.52392723802973 40.0 37.0 41.0 33.0 41.0 46-47 37.14023932230846 40.0 37.0 41.0 32.5 41.0 48-49 36.76366505971963 40.0 37.0 41.0 31.0 41.0 50-51 36.47521510715138 39.0 36.0 41.0 31.0 41.0 52-53 36.23860778102619 39.0 36.0 40.5 30.0 41.0 54-55 35.94556776638059 39.0 35.0 40.0 29.5 41.0 56-57 35.655612757199364 38.5 35.0 40.0 28.5 41.0 58-59 35.31012487587305 38.0 35.0 40.0 28.0 41.0 60-61 34.97030106679833 38.0 34.0 40.0 28.0 41.0 62-63 34.613457857860084 37.5 34.0 40.0 27.0 41.0 64-65 34.223138927876 37.0 34.0 39.5 26.0 41.0 66-67 33.82692625610926 36.0 33.0 39.0 26.0 40.0 68-69 33.517115372880134 36.0 33.0 39.0 26.0 40.0 70-71 33.04963746609046 35.0 33.0 38.5 25.0 40.0 72-73 32.54039798290238 35.0 32.0 37.5 23.5 39.5 74-75 32.05252997076429 35.0 31.5 37.0 22.5 39.0 76-77 30.65576087740418 33.5 30.0 35.5 21.0 37.5 78-79 31.373408817312868 34.0 31.0 36.0 21.5 38.0 80-81 31.08903522154234 34.0 31.0 36.0 20.0 37.0 82-83 30.801563860889054 34.0 31.0 35.0 19.0 37.0 84-85 30.48987956218552 34.0 31.0 35.0 18.0 36.5 86-87 30.188719009869132 34.0 31.0 35.0 17.5 36.0 88-89 29.839299563405987 34.0 30.0 35.0 11.5 36.0 90-91 29.43921024747724 34.0 30.0 35.0 7.0 35.0 92-93 29.0014795377762 34.0 29.0 35.0 2.0 35.0 94-95 28.44857510581996 34.0 29.0 35.0 2.0 35.0 96-97 27.818668138622762 33.0 28.5 35.0 2.0 35.0 98-99 27.08269268108666 33.0 27.0 34.5 2.0 35.0 100-101 23.597409838066337 29.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.005245443611961775 1101 2 0.01328425208902928 1101 3 0.0672671030131653 1101 4 0.02013573470959784 1101 5 0.09590916463492505 1101 6 0.008573328041038053 1101 7 0.12319413421289482 1101 8 0.08169495915776537 1101 9 0.08328505311919798 1101 10-11 0.055452152398295596 1101 12-13 0.03432321934759841 1101 14-15 0.12898402308717039 1101 16-17 0.13844356159787452 1101 18-19 0.15386775468653724 1101 20-21 0.2267656404249223 1101 22-23 0.13815293288045893 1101 24-25 0.05303553917018888 1101 26-27 -0.02050287703581688 1101 28-29 -0.019625582253475216 1101 30-31 0.11235246629070872 1101 32-33 0.17856106610204847 1101 34-35 0.12473299740508281 1101 36-37 0.18068459107626467 1101 38-39 0.1925962643468111 1101 40-41 0.31026236559714704 1101 42-43 0.22465897377310995 1101 44-45 0.23090459070199643 1101 46-47 0.056550773062966186 1101 48-49 0.10342684994617457 1101 50-51 0.12990530416338686 1101 52-53 0.09038947794577723 1101 54-55 0.10190148873432747 1101 56-57 0.07051825597420702 1101 58-59 0.031063907227597554 1101 60-61 0.0976725336056603 1101 62-63 -0.05528324788532757 1101 64-65 0.006160576821699237 1101 66-67 -0.142251654606099 1101 68-69 -0.202106430760864 1101 70-71 -0.3135410564790604 1101 72-73 -0.12900977361374544 1101 74-75 -0.17041866567262431 1101 76-77 -0.4571334575317003 1101 78-79 -0.09511989943699106 1101 80-81 -0.01608515810978517 1101 82-83 0.029038520790496847 1101 84-85 0.14531741415915533 1101 86-87 -0.21641966903672127 1101 88-89 -0.30377066233055317 1101 90-91 -0.13423459129520054 1101 92-93 -0.0382684349536504 1101 94-95 -0.2928069054772564 1101 96-97 -0.18166726266754196 1101 98-99 -0.5435136196888237 1101 100-101 -0.07739623975894361 1102 1 0.1703068293715546 1102 2 0.07872707720113681 1102 3 0.11646184215786803 1102 4 0.06260948511975073 1102 5 0.07116687545194367 1102 6 -0.006535224950326324 1102 7 -0.0027075118551351807 1102 8 0.032373757145300885 1102 9 0.16445507390012182 1102 10-11 0.04534674388906268 1102 12-13 0.11155238373564913 1102 14-15 0.1546864046905796 1102 16-17 0.15491996431033073 1102 18-19 0.154206470097229 1102 20-21 0.279564229509468 1102 22-23 0.24177592314624263 1102 24-25 0.30057464012173085 1102 26-27 0.07046596524789805 1102 28-29 0.11438165005539247 1102 30-31 0.09028879736366235 1102 32-33 -0.011502712198286247 1102 34-35 -0.12182276930168712 1102 36-37 -0.23965371418459114 1102 38-39 -0.07061261129575058 1102 40-41 -0.09531191253913107 1102 42-43 -0.14960685801076323 1102 44-45 -0.13278689458729787 1102 46-47 -0.05710790023901069 1102 48-49 -0.3181671974124569 1102 50-51 -0.23411122487522107 1102 52-53 -0.3200194414461066 1102 54-55 -0.16005198660199937 1102 56-57 -0.24275385110224335 1102 58-59 -0.08588690213475303 1102 60-61 -0.12551118617997048 1102 62-63 -0.104120473084933 1102 64-65 -0.2292367216580118 1102 66-67 -0.310169050428037 1102 68-69 -0.2864376814444469 1102 70-71 -0.1014088241701927 1102 72-73 -0.04818632944881074 1102 74-75 -0.051123166383547414 1102 76-77 -0.3339924616878811 1102 78-79 -0.335552129011532 1102 80-81 -0.03681105497077297 1102 82-83 0.014812131749700086 1102 84-85 -0.013115608371741416 1102 86-87 -0.13782333851063555 1102 88-89 -0.14099449477066983 1102 90-91 -0.27345072658996017 1102 92-93 -0.31012668716845937 1102 94-95 -0.2846069847728927 1102 96-97 -0.32528738188443285 1102 98-99 0.02568857051133122 1102 100-101 0.01930683909920461 1103 1 0.028736014227213502 1103 2 0.08620091875569358 1103 3 0.014819182976935963 1103 4 0.02340690634704856 1103 5 0.054728388934591976 1103 6 -0.0012967955275726695 1103 7 -0.08808217533402285 1103 8 0.01932367645952837 1103 9 -0.14736152556144333 1103 10-11 0.10199053306725148 1103 12-13 0.1282834800708983 1103 14-15 0.09715883870468645 1103 16-17 0.09571683045125212 1103 18-19 -0.07100912148607108 1103 20-21 -0.027225269842276134 1103 22-23 0.04475673698215843 1103 24-25 -0.04862821619415314 1103 26-27 -0.0806182373903539 1103 28-29 -0.05059557157870387 1103 30-31 -0.0428391540107782 1103 32-33 -0.015278092531573861 1103 34-35 0.023375535305923734 1103 36-37 -0.19641565746268697 1103 38-39 -0.08569391162523488 1103 40-41 -0.13490979210917686 1103 42-43 -0.12484960917743848 1103 44-45 -0.13093052715183262 1103 46-47 -0.2138964083650805 1103 48-49 -0.19841960311715923 1103 50-51 -0.27425388207370816 1103 52-53 -0.31467713157310584 1103 54-55 -0.4655858012745142 1103 56-57 -0.3940497795436855 1103 58-59 -0.242488663344524 1103 60-61 -0.26648488698047856 1103 62-63 -0.2605644311679782 1103 64-65 -0.34797643769167763 1103 66-67 -0.45878232571519817 1103 68-69 -0.6041239228356474 1103 70-71 -0.4299581059280726 1103 72-73 -0.4816473326484889 1103 74-75 -0.522004921090879 1103 76-77 -0.44597043268858627 1103 78-79 -0.2918723821309186 1103 80-81 -0.32895816871986483 1103 82-83 -0.3505976607669403 1103 84-85 -0.09688171083275776 1103 86-87 -0.2209800354295126 1103 88-89 -0.5041585664085311 1103 90-91 -0.583524354832754 1103 92-93 -0.6460189686924096 1103 94-95 -0.5415160259404779 1103 96-97 -0.4055592156122749 1103 98-99 -0.33588755760260725 1103 100-101 -0.24546936196894364 1104 1 -0.03591731956372968 1104 2 0.009183050610289456 1104 3 -0.005788101891930353 1104 4 -0.01593962257816628 1104 5 -0.03930368645384874 1104 6 -0.0958093643533573 1104 7 -0.06154308457796276 1104 8 -0.03994208380693465 1104 9 -0.05788024601068997 1104 10-11 0.04144100547075169 1104 12-13 -0.08111482447369411 1104 14-15 -0.17080450651023682 1104 16-17 0.0921102695911955 1104 18-19 -0.06695679673134691 1104 20-21 -0.14154225822707645 1104 22-23 -0.16537824244977628 1104 24-25 -0.16229514925684185 1104 26-27 -0.2653753185398102 1104 28-29 -0.37080522361319623 1104 30-31 -0.3103734872280697 1104 32-33 -0.28080097235428525 1104 34-35 -0.32293970107560455 1104 36-37 -0.30502882919333274 1104 38-39 -0.1609904202030279 1104 40-41 -0.20394109119019532 1104 42-43 -0.23408668692749046 1104 44-45 -0.14932370153009344 1104 46-47 0.0798096996217339 1104 48-49 -0.07191254525692159 1104 50-51 -0.04240926123041788 1104 52-53 0.0512611135798835 1104 54-55 -0.0460594632065181 1104 56-57 -0.10592287044028836 1104 58-59 -0.08000384219758416 1104 60-61 -0.08793254506527148 1104 62-63 -0.17453649390670023 1104 64-65 -0.1127728571984079 1104 66-67 -0.2518457328712742 1104 68-69 -0.23999569972979629 1104 70-71 0.05573332249392848 1104 72-73 0.050621724064441764 1104 74-75 -0.08062757194375436 1104 76-77 -0.11813907607909968 1104 78-79 -0.053508413255126897 1104 80-81 0.15347930584585256 1104 82-83 0.19212784836815544 1104 84-85 0.1111278205880808 1104 86-87 -0.18532464401530646 1104 88-89 -0.24763308963320085 1104 90-91 0.15815036091615298 1104 92-93 -0.05328128378823749 1104 94-95 -0.22201363082778158 1104 96-97 -0.31697028101658375 1104 98-99 -0.39955947537612246 1104 100-101 -0.10371521771304515 1105 1 0.19730253427780298 1105 2 0.2001249693886038 1105 3 0.20111113923834267 1105 4 0.10028521500186827 1105 5 0.11434013612818461 1105 6 0.0794735544483629 1105 7 0.1198076723419419 1105 8 0.1528814107374714 1105 9 0.21126308131719895 1105 10-11 0.20639573066319628 1105 12-13 0.16949546901312829 1105 14-15 0.13620236465349222 1105 16-17 0.10844780673526344 1105 18-19 0.10677406579078053 1105 20-21 0.20163571349274179 1105 22-23 0.07236616741787572 1105 24-25 0.1718093376676677 1105 26-27 0.2359018131977919 1105 28-29 0.13056062490922926 1105 30-31 0.09726454049056343 1105 32-33 0.15490568120959836 1105 34-35 0.26068513026501705 1105 36-37 0.2186351545217775 1105 38-39 0.3468459041533336 1105 40-41 0.3077490513026149 1105 42-43 0.22080618999211055 1105 44-45 0.17485912913978297 1105 46-47 0.16937173117761262 1105 48-49 -0.010286551823909917 1105 50-51 0.18186482098956702 1105 52-53 0.09110664888331144 1105 54-55 0.04790623556976925 1105 56-57 -0.03400378014805483 1105 58-59 -0.004038876553401849 1105 60-61 -0.092963079237812 1105 62-63 -0.09260508075556118 1105 64-65 0.10401099361700972 1105 66-67 0.0025043143143506086 1105 68-69 -0.058021000586940374 1105 70-71 -0.15432267632058227 1105 72-73 0.03661812079811 1105 74-75 0.25453566694032403 1105 76-77 -0.08738359903627568 1105 78-79 0.25601567481431786 1105 80-81 0.10357465201679972 1105 82-83 0.09643168483289699 1105 84-85 0.31870072433719443 1105 86-87 0.025176888542780773 1105 88-89 0.32510784955583105 1105 90-91 0.047407440710564686 1105 92-93 -0.29823807578929973 1105 94-95 -0.24048719517572437 1105 96-97 -0.09602161586297342 1105 98-99 -0.25131470284205903 1105 100-101 -0.10423386968587423 1106 1 0.1601659525773229 1106 2 0.11588128405249876 1106 3 0.06572879139717713 1106 4 0.11824660451692637 1106 5 0.06271017254226052 1106 6 0.14172429139998144 1106 7 0.1184782694172597 1106 8 0.13986193209461817 1106 9 0.09466577480312566 1106 10-11 0.07461144073776182 1106 12-13 0.10901486095579571 1106 14-15 0.2611517895422253 1106 16-17 0.20144821133614954 1106 18-19 0.018953997586635296 1106 20-21 0.07954016988254864 1106 22-23 -0.06230090384968179 1106 24-25 0.11387193520538119 1106 26-27 0.09874933646234041 1106 28-29 0.12350178437977632 1106 30-31 0.07963550170667588 1106 32-33 0.1676867343279156 1106 34-35 -0.07058907353822974 1106 36-37 0.06959030162310142 1106 38-39 -0.062328421030180436 1106 40-41 -0.052634741541730534 1106 42-43 0.015232812730680223 1106 44-45 -0.06577725026025405 1106 46-47 -0.024085294427841575 1106 48-49 -0.0662659749786485 1106 50-51 0.18700903230683252 1106 52-53 0.12867673153749593 1106 54-55 0.002229685081360344 1106 56-57 0.08934169121198465 1106 58-59 0.22246537175593772 1106 60-61 0.14999253026452664 1106 62-63 0.3166992947140699 1106 64-65 0.053862158289575746 1106 66-67 0.33113416230001746 1106 68-69 0.2134517466530994 1106 70-71 0.17198071654689073 1106 72-73 0.18953558221652145 1106 74-75 0.41927325436301643 1106 76-77 0.35430329767489255 1106 78-79 0.2649024443652088 1106 80-81 -0.028570888703391972 1106 82-83 0.22928527711080093 1106 84-85 0.30781985039927307 1106 86-87 0.288832168584392 1106 88-89 0.3246887986339182 1106 90-91 0.17094798749976903 1106 92-93 0.09488948909134365 1106 94-95 -0.07186254920150148 1106 96-97 0.1232142664559639 1106 98-99 0.07607966742555661 1106 100-101 -0.03610196979569125 1107 1 0.1697728658294011 1107 2 0.1924118706116289 1107 3 0.126883030622281 1107 4 0.15235095777142504 1107 5 0.08736871367087673 1107 6 0.16323255034990325 1107 7 0.16847625565318225 1107 8 0.10610621174603807 1107 9 0.18721082018833357 1107 10-11 0.22579477885378196 1107 12-13 0.20361709220639312 1107 14-15 0.23994307799883074 1107 16-17 0.14220075497190265 1107 18-19 0.1242669365242719 1107 20-21 0.12711219970638865 1107 22-23 0.22443644593609946 1107 24-25 0.25683659868023057 1107 26-27 0.2996732765931327 1107 28-29 0.3174247478349983 1107 30-31 0.14473231341469983 1107 32-33 0.20184835399835777 1107 34-35 0.06852930054865425 1107 36-37 0.27332407194324304 1107 38-39 0.12094235975649781 1107 40-41 0.19653772728268137 1107 42-43 0.313451764264407 1107 44-45 0.24009715127424158 1107 46-47 0.21826303137904546 1107 48-49 0.059287180841380405 1107 50-51 0.11728622693209445 1107 52-53 0.12314706124101349 1107 54-55 0.4036345372459067 1107 56-57 0.3641965743381519 1107 58-59 0.32703007147854635 1107 60-61 0.18015179383100843 1107 62-63 0.2544192355094026 1107 64-65 -0.09105585634042512 1107 66-67 0.3023568443317046 1107 68-69 0.24491932756865964 1107 70-71 0.25654055806365506 1107 72-73 0.1984257213172782 1107 74-75 0.2326413754707879 1107 76-77 0.19512783337641437 1107 78-79 0.5004750833526899 1107 80-81 0.5568372509310073 1107 82-83 0.33137241771684245 1107 84-85 0.3293463590861485 1107 86-87 0.3766782213550819 1107 88-89 0.4561403952971119 1107 90-91 0.4895179129401548 1107 92-93 0.4821822601817445 1107 94-95 0.41917871785885197 1107 96-97 0.29515007269158744 1107 98-99 0.25694966385112394 1107 100-101 0.41622468287531333 1108 1 0.02483038541461724 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-0.59319938574723 2316 96-97 -0.4156812522400877 2316 98-99 -0.41655121833897013 2316 100-101 -0.7903990876680957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 17.0 6 20.0 7 72.0 8 165.0 9 330.0 10 558.0 11 951.0 12 1549.0 13 2337.0 14 2832.0 15 4118.0 16 6025.0 17 9144.0 18 8286.0 19 4625.0 20 3811.0 21 3805.0 22 3911.0 23 4155.0 24 4829.0 25 5776.0 26 6933.0 27 8368.0 28 10446.0 29 13622.0 30 17196.0 31 22682.0 32 29547.0 33 40740.0 34 58244.0 35 89186.0 36 145594.0 37 225953.0 38 155907.0 39 9557.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.844828026311845 19.36120412102029 14.802355946856908 21.99161190581096 2 15.726029707595332 20.8951779249066 39.68406137690832 23.694730990589747 3 19.85278943385843 25.0624102679037 29.729810694401603 25.354989603836266 4 13.351788260225565 17.259942638092966 37.21411968334452 32.17414941833695 5 14.107589634914206 37.41538563955198 33.56559173189688 14.911432993636934 6 31.680082547889427 36.49970320483305 17.310980311662664 14.509233935614866 7 28.573108693601984 33.00495398288019 20.67891200994125 17.743025313576577 8 26.63556327284629 34.41448138511808 19.521799188944797 19.428156153090832 9 26.882097426480783 17.055237186492768 20.25840596031266 35.804259426713784 10-11 25.04366603858591 26.83930010053731 27.079879444004266 21.037154416872514 12-13 26.436017064335203 24.122845461252975 27.730043992255588 21.71109348215623 14-15 23.238506815193695 25.507741638420274 26.312084278732268 24.941667267653763 16-17 22.268402687244464 28.860750364752942 26.679000771112676 22.19184617688992 18-19 22.05386495593543 27.257289820934638 29.44437479127163 21.2444704318583 20-21 23.02164738959254 26.182351560796707 29.523464514335895 21.272536535274856 22-23 22.749039966891203 26.140694641234436 29.422005146065942 21.68826024580842 24-25 22.638344855135987 26.267324315627118 29.561357760294722 21.532973068942177 26-27 22.539927000455485 26.257034070337653 29.654691849049946 21.54834708015692 28-29 22.045240836026238 26.55240419954787 29.810521060150062 21.591833904275827 30-31 22.32375863232083 26.30500053258535 29.505505601022563 21.86573523407126 32-33 22.404005066249027 26.45461873591206 29.422579648347284 21.71879654949163 34-35 22.737283575299987 26.265373712269568 29.343333758647276 21.65400895378317 36-37 22.76610181528067 26.05024020470245 29.35618260106823 21.827475378948648 38-39 22.385197818665624 26.428622551366722 29.500111796438382 21.686067833529275 40-41 22.53055359847329 26.504435333995353 29.223830419900477 21.741180647630884 42-43 22.460626099112943 26.61720080550763 28.84965521832474 22.072517877054683 44-45 22.729794351460953 26.40328638237203 28.791461175308864 22.075458090858156 46-47 23.01049272654448 26.949009809262492 28.15422543884273 21.886272025350305 48-49 23.073863280172958 27.136646041986474 27.8781581252604 21.911332552580173 50-51 22.8418685109589 27.116910092972834 27.967025102332254 22.074196293736012 52-53 22.960961727292396 27.384430181017315 27.439129252908316 22.215478838781973 54-55 22.75837352295177 27.15161245760742 27.68803498702078 22.401979032420027 56-57 22.828550917444936 27.468868342185825 27.600851259302328 22.101729481066915 58-59 23.058914770826593 27.1055433108083 27.585908474875275 22.249633443489827 60-61 22.85772457401919 27.07170857220842 28.210042905978543 21.86052394779384 62-63 22.791141765155913 27.066914991059544 28.113250569631525 22.028692674153014 64-65 22.669227427882895 27.291301566801973 27.804008567676824 22.235462437638308 66-67 22.533015636138703 27.324547655966114 27.647311732513014 22.495124975382165 68-69 22.441389563847373 27.223146822887195 27.900176413806875 22.435287199458553 70-71 22.726723008320324 27.179466695958116 27.69500725074255 22.39880304497901 72-73 22.66133729799899 27.16813030139965 27.752955469629807 22.417576930971546 74-75 22.686134950266005 27.624085343866327 27.34659573169717 22.3431839741705 76-77 22.657509472481262 27.485118634853183 27.284851241824263 22.57252065084129 78-79 22.80393616655368 27.326267110330495 27.257143427757985 22.61265329535784 80-81 22.893846151285697 27.5707423263431 27.085155969583308 22.450255552787894 82-83 22.81422447704618 27.509462603988776 27.18904901246034 22.4872639065047 84-85 22.700657438486505 27.470942328497323 27.14721628894005 22.681183944076118 86-87 22.622185409705374 27.333848535972 27.230992933809034 22.81297312051359 88-89 22.56673325614797 27.39207594418466 27.065212386420185 22.975978413247184 90-91 22.675886784497777 27.284392370934995 27.176713377492263 22.86300746707497 92-93 22.84286177905619 27.206816876874047 27.108458130961928 22.841863213107843 94-95 22.563755485163014 27.2163941883623 27.16973909763174 23.050111228842944 96-97 22.352426221432236 27.246421193017028 27.29080261584106 23.110349969709677 98-99 22.374776775931576 27.468529497753476 26.839535527507806 23.317158198807142 100-101 22.079901918633517 27.803016223922867 26.6901144744908 23.426967382952814 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 195.0 1 141.5 2 119.0 3 148.0 4 128.5 5 121.5 6 204.5 7 437.5 8 757.0 9 969.0 10 942.5 11 897.5 12 998.0 13 1111.5 14 1216.0 15 1334.0 16 1439.5 17 1459.0 18 1512.5 19 1629.5 20 1752.5 21 1885.5 22 2119.5 23 2474.0 24 2867.0 25 3491.5 26 4371.5 27 5425.5 28 6593.0 29 7922.5 30 9458.5 31 11276.0 32 12951.5 33 14642.5 34 16888.0 35 18668.5 36 20884.5 37 23232.0 38 24764.0 39 25869.5 40 26395.5 41 27746.5 42 29013.0 43 30049.0 44 31798.0 45 33002.0 46 33556.5 47 35291.0 48 38858.5 49 38255.0 50 34712.0 51 32967.5 52 31662.5 53 30789.5 54 29634.5 55 28136.0 56 25930.5 57 23233.0 58 21150.0 59 19056.5 60 16255.0 61 12971.5 62 9935.5 63 7611.5 64 5677.5 65 4204.5 66 3138.0 67 2432.0 68 1674.0 69 980.5 70 657.0 71 426.5 72 313.0 73 211.0 74 125.5 75 96.5 76 70.5 77 33.0 78 11.0 79 7.5 80 6.0 81 5.5 82 5.5 83 3.0 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028403574856179163 2 0.004881864428405794 3 1.1095146428194986E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01520035060662713 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.438058571277994E-4 20-21 9.430874463965738E-4 22-23 0.0026628351427667963 24-25 0.011649903749604736 26-27 0.006268757731930167 28-29 8.321359821146239E-4 30-31 0.003439495392740446 32-33 0.00404972844629117 34-35 0.0 36-37 0.009375398731824764 38-39 0.01148347655318181 40-41 0.0011095146428194986 42-43 0.0 44-45 0.0 46-47 4.438058571277994E-4 48-49 0.003162116732035571 50-51 0.007100893714044791 52-53 0.0 54-55 0.0051592430891106685 56-57 0.004659961499841894 58-59 1.6642719642292477E-4 60-61 0.002440932214202897 62-63 0.0060468548033662676 64-65 5.547573214097493E-5 66-67 0.0019416506249341227 68-69 5.547573214097493E-4 70-71 8.876117142555988E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0013314175713833982 80-81 0.0014978447678063232 82-83 0.012870369856706185 84-85 0.007766602499736491 86-87 0.0034949711248814207 88-89 1.6642719642292477E-4 90-91 5.547573214097493E-4 92-93 2.7737866070487465E-4 94-95 0.0 96-97 0.0018861748927931476 98-99 0.001608796232088273 100-101 2.7737866070487465E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 901295.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.14072925922308 #Duplication Level Percentage of deduplicated Percentage of total 1 80.30889755031824 48.298356646803725 2 11.287199229504251 13.57640785913053 3 3.3516842868940113 6.047182117814547 4 1.5355227013690476 3.6938982021770697 5 0.8722433360381143 2.6228675160414885 6 0.5519464859377651 1.9916678505817624 7 0.3811585585880372 1.6046207573815208 8 0.27219478313173395 1.3095994206478845 9 0.20980103522300944 1.1355828531886563 >10 1.1312172590493588 12.304786152388417 >50 0.06827043724994904 2.7655702476835327 >100 0.027638423460073544 2.825731372366649 >500 0.0014839421575385612 0.5410500164180485 >1k 7.419710787692806E-4 1.282678987376231 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4077 0.45234911987750953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3238 0.3592608413449537 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2199 0.24398226995600772 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1984 0.22012770513538854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.1095146428194986E-4 0.0 0.0 0.0 0.0 14-15 1.1095146428194986E-4 0.0 0.0 0.0 0.0 16-17 1.1095146428194986E-4 0.0 0.0 1.1095146428194986E-4 0.0 18-19 1.1095146428194986E-4 0.0 0.0 1.1095146428194986E-4 0.0 20-21 1.1095146428194986E-4 0.0 0.0 1.1095146428194986E-4 0.0 22-23 1.1095146428194986E-4 0.0 0.0 1.1095146428194986E-4 0.0 24-25 1.1095146428194986E-4 0.0 0.0 1.1095146428194986E-4 0.0 26-27 1.1095146428194986E-4 0.0 0.0 2.7737866070487465E-4 0.0 28-29 1.1095146428194986E-4 0.0 0.0 0.0020526020892160726 0.0 30-31 1.1095146428194986E-4 0.0 0.0 0.00438258283913702 0.0 32-33 1.1095146428194986E-4 0.0 0.0 0.007156369446185766 0.0 34-35 1.1095146428194986E-4 0.0 0.0 0.010429437642503286 0.0 36-37 1.1095146428194986E-4 0.0 0.0 0.013591554374538857 0.0 38-39 1.1095146428194986E-4 0.0 0.0 0.01692009830299735 0.0 40-41 1.1095146428194986E-4 0.0 0.0 0.022745050177799722 0.0 42-43 1.1095146428194986E-4 0.0 0.0 0.031676643052496685 0.0 44-45 1.1095146428194986E-4 0.0 0.0 0.05087124637327401 0.0 46-47 1.1095146428194986E-4 0.0 0.0 0.0911466279076218 0.0 48-49 1.1095146428194986E-4 0.0 0.0 0.11755307640672588 0.0 50-51 1.1095146428194986E-4 0.0 0.0 0.14640045712003286 0.0 52-53 1.1095146428194986E-4 0.0 0.0 0.1785763817617983 0.0 54-55 1.1095146428194986E-4 0.0 0.0 0.2173539185283398 0.0 56-57 1.1095146428194986E-4 0.0 0.0 0.26350972766963093 0.0 58-59 1.1095146428194986E-4 0.0 0.0 0.3312455966137613 0.0 60-61 1.1095146428194986E-4 0.0 0.0 0.41795416595010515 0.0 62-63 1.1095146428194986E-4 0.0 0.0 0.5206952218751907 0.0 64-65 1.1095146428194986E-4 0.0 0.0 0.5994152857832342 0.0 66-67 1.1095146428194986E-4 0.0 0.0 0.7220166538147887 0.0 68-69 1.1095146428194986E-4 0.0 0.0 0.8808991506665409 0.0 70-71 1.1095146428194986E-4 0.0 0.0 0.9875235078414948 0.0 72-73 1.1095146428194986E-4 0.0 0.0 1.17958049251355 0.0 74-75 1.1095146428194986E-4 0.0 0.0 1.3356337270261123 0.0 76-77 1.1095146428194986E-4 0.0 0.0 1.4595665126290505 0.0 78-79 1.1095146428194986E-4 0.0 0.0 1.6175059220344061 0.0 80-81 1.1095146428194986E-4 0.0 0.0 1.757082864101099 0.0 82-83 1.1095146428194986E-4 0.0 0.0 1.936768760505717 0.0 84-85 1.1095146428194986E-4 0.0 0.0 2.2473773847630354 0.0 86-87 1.1095146428194986E-4 0.0 0.0 2.6321015871606965 0.0 88-89 1.1095146428194986E-4 0.0 0.0 3.10175913546619 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGT 90 5.689453E-7 36.94999 8 CGTCGAT 45 8.946033E-4 26.390654 44-45 GTATCAA 6870 0.0 25.862688 1 ATACGTC 150 1.0583008E-6 25.337137 5 GTATAGG 350 0.0 24.432238 1 GAGTACT 3730 0.0 22.727077 12-13 GGTATCA 5005 0.0 22.306044 1 TCAACGC 8035 0.0 21.869057 4 TATCAAC 8125 0.0 21.744925 2 ATCAACG 8135 0.0 21.600231 3 CAACGCA 8175 0.0 21.501698 5 AGTACTT 3930 0.0 21.44964 12-13 AACGCAG 8275 0.0 21.24186 6 GTACTTT 4005 0.0 21.107252 14-15 TTGCGTA 155 2.6011548E-10 19.920687 60-61 GTACATG 4115 0.0 19.857172 1 GCGTCGA 60 0.004762687 19.792992 44-45 AGAGTAC 6550 0.0 19.546827 10-11 ACGCAGA 9095 0.0 19.117767 7 GTACTCG 125 0.0060249274 19.002853 9 >>END_MODULE