FastQCFastQC Report
Thu 26 May 2016
SRR936936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925923
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.33836506923361875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23010.24850878528776152No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15260.1648085207949257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13880.1499044736981369No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11500.12420039247324022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG40500.026.1669271
GTATCAA55350.025.3283731
TACATGG41850.025.097082
ACATGGG41800.023.8764293
CCCTATA2402.2737368E-1023.7627332
CCTATAC2402.2737368E-1023.7627333
CACCGTA1201.7073865E-423.7563117
GGTATCA41300.023.473781
CATGGGG19750.021.897814
CAACGCA64200.021.4678885
TGCACCG2005.356069E-721.3864575
TCAACGC64450.021.3846174
TATCAAC65200.021.3573042
ATCAACG64550.021.3514863
CGATTCG905.1838833E-621.10531238-39
AACGCAG65750.021.0329466
GAGTACT25950.020.9630112-13
GTACTTT27200.020.08859814-15
TATACAG5150.019.3793165
TACCTGG5200.019.1929762
Produced by FastQC (version 0.11.5)