##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936936_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 925923 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.940419451725468 33.0 31.0 34.0 30.0 34.0 2 32.04324981666942 33.0 31.0 34.0 30.0 34.0 3 32.075203877644256 34.0 31.0 34.0 30.0 34.0 4 35.64892113059077 37.0 35.0 37.0 33.0 37.0 5 35.63842133741143 37.0 35.0 37.0 33.0 37.0 6 35.70788823692683 37.0 35.0 37.0 35.0 37.0 7 35.66152369041486 37.0 35.0 37.0 33.0 37.0 8 35.62292868845466 37.0 35.0 37.0 33.0 37.0 9 37.28037644598957 39.0 37.0 39.0 34.0 39.0 10-11 37.15038237520831 39.0 37.0 39.0 33.5 39.0 12-13 37.08700507493604 39.0 37.0 39.0 33.0 39.0 14-15 38.29665101741722 40.0 38.0 41.0 33.5 41.0 16-17 38.230328007836505 40.0 38.0 41.0 33.0 41.0 18-19 38.17500213300674 40.0 38.0 41.0 33.5 41.0 20-21 38.10221368299524 40.0 38.0 41.0 33.0 41.0 22-23 37.992326035750274 40.0 38.0 41.0 33.0 41.0 24-25 37.96148005827698 40.0 38.0 41.0 33.0 41.0 26-27 37.98937870643671 40.0 38.0 41.0 33.0 41.0 28-29 37.91383354771401 40.0 38.0 41.0 33.0 41.0 30-31 37.77600243216769 40.0 37.5 41.0 33.0 41.0 32-33 37.59562188216515 40.0 37.0 41.0 33.0 41.0 34-35 37.44109337385506 40.0 37.0 41.0 32.0 41.0 36-37 37.281907350827225 40.0 37.0 41.0 31.5 41.0 38-39 37.13324434105212 40.0 36.5 41.0 31.0 41.0 40-41 36.95832320830134 39.0 36.0 41.0 31.0 41.0 42-43 36.74456353282076 39.0 36.0 41.0 30.5 41.0 44-45 36.504166113164914 39.0 36.0 41.0 30.0 41.0 46-47 36.18150213354674 39.0 35.0 40.5 29.5 41.0 48-49 35.97884489314986 39.0 35.0 40.5 29.0 41.0 50-51 35.3278091158768 38.5 34.5 40.0 28.0 40.5 52-53 35.52530069995021 38.5 35.0 40.0 28.5 40.5 54-55 35.90263337232145 39.0 35.0 40.5 29.0 41.0 56-57 35.77270140173643 39.0 35.0 41.0 28.0 41.0 58-59 35.54864227370959 39.0 35.0 41.0 28.0 41.0 60-61 35.25571942807339 38.0 35.0 40.0 27.5 41.0 62-63 34.94082175299674 38.0 34.0 40.0 27.0 41.0 64-65 34.59464339907314 37.5 34.0 40.0 26.0 41.0 66-67 34.22200604153909 37.0 34.0 40.0 26.0 41.0 68-69 33.825496288568274 36.0 34.0 39.0 26.0 41.0 70-71 33.39635963249644 36.0 33.0 39.0 25.0 40.5 72-73 32.99535652532661 35.0 33.0 38.5 24.5 40.0 74-75 32.59318053445048 35.0 32.5 37.5 23.5 39.5 76-77 32.13960880116381 35.0 32.0 37.0 22.0 39.0 78-79 31.681711114207122 35.0 32.0 36.5 20.5 39.0 80-81 31.24256444650365 35.0 31.0 36.0 19.5 37.5 82-83 30.855547383529732 35.0 31.0 35.5 18.0 37.0 84-85 30.491882154347607 34.0 31.0 35.0 16.0 37.0 86-87 30.155160310306577 34.0 31.0 35.0 11.5 36.0 88-89 29.79151236117906 34.0 30.0 35.0 7.0 36.0 90-91 29.425060183190176 34.0 30.0 35.0 2.0 36.0 92-93 29.034246908220233 34.0 29.0 35.0 2.0 35.0 94-95 28.66806311107943 34.0 29.0 35.0 2.0 35.0 96-97 28.19631600035856 34.0 29.0 35.0 2.0 35.0 98-99 27.63792345583812 34.0 29.0 35.0 2.0 35.0 100-101 25.461085857031307 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18716014386018642 1101 2 0.1652225189688501 1101 3 0.1944054866580771 1101 4 0.19896365611862876 1101 5 0.2365400776039337 1101 6 0.27692631016756053 1101 7 0.16978415105486278 1101 8 0.2718125529030644 1101 9 0.3335762578470636 1101 10-11 0.19024094016615578 1101 12-13 0.2748213332159324 1101 14-15 0.2875608690385718 1101 16-17 0.3546674231494009 1101 18-19 0.36562928849762955 1101 20-21 0.2665695353599773 1101 22-23 0.31198043092235395 1101 24-25 0.21989643244982204 1101 26-27 0.27624021843378443 1101 28-29 0.18849413970968953 1101 30-31 0.15007486870999287 1101 32-33 0.11396658296337137 1101 34-35 0.18764663895715472 1101 36-37 0.08179359388506668 1101 38-39 0.03050773613193769 1101 40-41 0.04781973848211862 1101 42-43 0.15933628249809573 1101 44-45 0.23147981966463504 1101 46-47 0.19440885418622855 1101 48-49 0.12057465483097474 1101 50-51 0.18439561764463264 1101 52-53 0.1962238956113893 1101 54-55 0.38211800648734595 1101 56-57 0.14200362580473325 1101 58-59 0.11506412511878494 1101 60-61 0.2066816402350682 1101 62-63 0.2624833740875019 1101 64-65 0.027667500661046063 1101 66-67 0.06339953743298565 1101 68-69 0.004673078563307342 1101 70-71 -0.15572614349922986 1101 72-73 -0.1578480962980322 1101 74-75 -0.1863262751026653 1101 76-77 -0.013187813374749169 1101 78-79 -0.11029072580489085 1101 80-81 -0.18581984052913114 1101 82-83 -0.234314200267697 1101 84-85 -0.14927453144581193 1101 86-87 0.04005762125457224 1101 88-89 0.17933272058397876 1101 90-91 0.007565966999887763 1101 92-93 0.04023064419143907 1101 94-95 0.1853541373487282 1101 96-97 0.17181428366400908 1101 98-99 0.08552147259165466 1101 100-101 0.12849887164022178 1102 1 0.10796891437632183 1102 2 0.03891309108810859 1102 3 0.22816433987492246 1102 4 0.16319029846987831 1102 5 0.0576944946699669 1102 6 0.12082342200437068 1102 7 0.023096420447117794 1102 8 -0.07155442618849861 1102 9 0.04091117451019244 1102 10-11 -0.0208367136783707 1102 12-13 0.09959655693194946 1102 14-15 0.06556248064211445 1102 16-17 0.21639820053605519 1102 18-19 0.05884146882758756 1102 20-21 -0.018997070518132375 1102 22-23 -0.0920284328971519 1102 24-25 -0.2393575647510815 1102 26-27 -0.16129954152210502 1102 28-29 -0.15635190974109747 1102 30-31 -0.11348062558577965 1102 32-33 -0.07240688495299707 1102 34-35 -0.11002289749476546 1102 36-37 -0.15604516093914356 1102 38-39 -0.11164205817656381 1102 40-41 -0.2373598704919715 1102 42-43 0.01410641693976089 1102 44-45 0.14271969412919816 1102 46-47 0.13484313893112443 1102 48-49 0.19193052234019348 1102 50-51 0.09814832132163076 1102 52-53 0.22982370900466975 1102 54-55 -0.08788178145955783 1102 56-57 0.12974695673479886 1102 58-59 -0.09025098578498358 1102 60-61 0.03086311527641783 1102 62-63 -0.014383753441229885 1102 64-65 0.21706802655273094 1102 66-67 0.19296928169694638 1102 68-69 0.13213819582866648 1102 70-71 0.029041022199059796 1102 72-73 -0.07221045263205639 1102 74-75 0.09106568384385838 1102 76-77 -0.0517973812105339 1102 78-79 0.13362335027975902 1102 80-81 -0.04790580685510193 1102 82-83 0.02713666702947748 1102 84-85 -0.00825922121212841 1102 86-87 -0.09709832259376938 1102 88-89 0.010665686358606763 1102 90-91 -0.09705042894565352 1102 92-93 -0.10431535012553894 1102 94-95 -0.18473153210290505 1102 96-97 -0.017405997094289916 1102 98-99 -0.007866711809722204 1102 100-101 0.16234254609630838 1103 1 0.20145385018711082 1103 2 0.2289164573873741 1103 3 0.2615748600701693 1103 4 0.2638911007711968 1103 5 0.15298116565502085 1103 6 0.1901449596890643 1103 7 0.3003047892711095 1103 8 0.24724814035966602 1103 9 0.16660037327996946 1103 10-11 0.19189937355978515 1103 12-13 0.20275386511664095 1103 14-15 0.34893092209657084 1103 16-17 0.49021859316896865 1103 18-19 0.2922790214260118 1103 20-21 0.30905453076921674 1103 22-23 0.3347156671533469 1103 24-25 0.34110846367884307 1103 26-27 0.3726146484493782 1103 28-29 0.3357749028325472 1103 30-31 0.442289644891261 1103 32-33 0.515688146695318 1103 34-35 0.406267950761638 1103 36-37 0.3546084111634116 1103 38-39 0.5237813559649638 1103 40-41 0.38628149622553565 1103 42-43 0.46364826871037934 1103 44-45 0.6135329869309345 1103 46-47 0.5605027550710062 1103 48-49 0.5476680224039541 1103 50-51 0.5233304075090999 1103 52-53 0.4985398228671869 1103 54-55 0.6452848095503896 1103 56-57 0.5726065769550317 1103 58-59 0.6627601944089392 1103 60-61 0.7218489564176949 1103 62-63 0.7198124916049551 1103 64-65 0.7002205913157411 1103 66-67 0.5477306824854793 1103 68-69 0.5794454114401404 1103 70-71 0.4963974826884012 1103 72-73 0.39035604452954686 1103 74-75 0.5445680377031081 1103 76-77 0.6188389258571618 1103 78-79 0.5757077015785903 1103 80-81 0.47290723092395837 1103 82-83 0.5710655973113781 1103 84-85 0.30827491867290746 1103 86-87 0.3694554540369772 1103 88-89 0.3847278404841106 1103 90-91 0.5476124565848686 1103 92-93 0.5720494037010724 1103 94-95 0.5439549481251866 1103 96-97 0.5403024456862724 1103 98-99 0.3390482789329603 1103 100-101 0.6469468274169152 1104 1 0.28232821137340025 1104 2 0.11546532177045421 1104 3 0.17587805873228035 1104 4 0.3208011888114868 1104 5 0.18249038855234545 1104 6 0.16096429180368688 1104 7 0.10305188288281641 1104 8 0.18277350138090043 1104 9 0.22688476800689017 1104 10-11 0.31260830496186287 1104 12-13 0.12057851951102805 1104 14-15 0.02825422334258576 1104 16-17 0.3017043158187036 1104 18-19 0.3000319854282196 1104 20-21 0.29221647569031717 1104 22-23 0.1458751704902852 1104 24-25 0.2777098034358687 1104 26-27 0.3212292674345676 1104 28-29 0.2673965320294016 1104 30-31 0.2396529519920989 1104 32-33 0.24502421642995387 1104 34-35 0.17998775406463352 1104 36-37 0.14811392185625039 1104 38-39 0.35169638634462075 1104 40-41 0.28230162060929587 1104 42-43 0.41508129798759086 1104 44-45 0.3546308589533069 1104 46-47 0.19982643002888523 1104 48-49 0.1943841851305521 1104 50-51 0.0957978089348046 1104 52-53 0.25171793082631666 1104 54-55 0.3754383739829805 1104 56-57 0.5242599405175099 1104 58-59 0.40073835095060417 1104 60-61 0.42743252306141954 1104 62-63 0.28518403905234635 1104 64-65 0.42797694986735024 1104 66-67 0.1627623078517928 1104 68-69 0.4710728643135198 1104 70-71 0.3235705393088679 1104 72-73 0.2899513457257328 1104 74-75 0.5505700059189209 1104 76-77 0.5767068062569081 1104 78-79 0.4569420718585313 1104 80-81 0.3985035349136439 1104 82-83 0.1641774648429788 1104 84-85 0.38429024708777604 1104 86-87 0.23625598221640587 1104 88-89 0.23451669328212432 1104 90-91 0.30546585564996676 1104 92-93 0.22301965312260208 1104 94-95 0.48433856335145364 1104 96-97 0.30411966321460326 1104 98-99 0.35930190471658463 1104 100-101 0.49214291714513436 1105 1 0.30764662843001744 1105 2 0.30690189144000257 1105 3 0.36536848814338185 1105 4 0.30987016109654775 1105 5 0.3022596201161747 1105 6 0.32365294035182757 1105 7 0.43646578890200516 1105 8 0.4338669140473357 1105 9 0.3463781930691283 1105 10-11 0.38865084320806176 1105 12-13 0.37059039881727784 1105 14-15 0.37162247150645555 1105 16-17 0.5314475162071091 1105 18-19 0.4872390055817206 1105 20-21 0.4959181708156706 1105 22-23 0.5616059544211964 1105 24-25 0.4777438918798893 1105 26-27 0.40627477034241366 1105 28-29 0.3941611254988828 1105 30-31 0.5204807846901502 1105 32-33 0.361012729657304 1105 34-35 0.34426409771746336 1105 36-37 0.4688309154620782 1105 38-39 0.6937708231105617 1105 40-41 0.722552325620299 1105 42-43 0.5612959312269226 1105 44-45 0.35946883554591125 1105 46-47 0.4203208157654572 1105 48-49 0.2625306001625205 1105 50-51 0.506884300614125 1105 52-53 0.5209748027467356 1105 54-55 0.44406047775646584 1105 56-57 0.4374547508101472 1105 58-59 0.7384396535423647 1105 60-61 0.7575202233310279 1105 62-63 0.849935554955124 1105 64-65 0.5608511221896038 1105 66-67 0.3408253611433878 1105 68-69 0.4512452692015927 1105 70-71 0.5923837449654812 1105 72-73 0.6119637002042282 1105 74-75 0.6632886109784693 1105 76-77 0.6374415671729565 1105 78-79 0.6630444198292373 1105 80-81 0.4698981654672174 1105 82-83 0.4654903864292308 1105 84-85 0.7558393086225834 1105 86-87 0.5652864242298961 1105 88-89 0.591582298042109 1105 90-91 0.7656092337114693 1105 92-93 0.3541723590219661 1105 94-95 0.745985448390762 1105 96-97 0.9290755031848512 1105 98-99 0.8199090922133578 1105 100-101 0.9025936076943744 1106 1 0.37586695934527015 1106 2 0.320759205657172 1106 3 0.2735575934536385 1106 4 0.266470378152583 1106 5 0.12105591112358383 1106 6 0.06499069096586396 1106 7 0.2010475752350942 1106 8 0.4222563201896392 1106 9 0.4006726657562467 1106 10-11 0.35937493452392033 1106 12-13 0.2994150594292222 1106 14-15 0.2578851093084751 1106 16-17 0.3781754916835851 1106 18-19 0.32506387563370254 1106 20-21 0.39873799526684195 1106 22-23 0.28517938219294336 1106 24-25 0.134679212989532 1106 26-27 0.20503145814348755 1106 28-29 0.08762469245305482 1106 30-31 0.15600181437351068 1106 32-33 0.3290540845439125 1106 34-35 0.4864872084731644 1106 36-37 0.2330642697394154 1106 38-39 0.2828475733628366 1106 40-41 0.43614688779543087 1106 42-43 0.4853396470739213 1106 44-45 0.4093003315189492 1106 46-47 0.43382588992626125 1106 48-49 0.6416380048197041 1106 50-51 0.8294048162174121 1106 52-53 0.5353481010464591 1106 54-55 0.7014089669322274 1106 56-57 0.7105893065116788 1106 58-59 0.8785398039583825 1106 60-61 0.7851110917455699 1106 62-63 0.6940417220121091 1106 64-65 0.20341885700044315 1106 66-67 0.2637609488130863 1106 68-69 0.6350022426027024 1106 70-71 0.6222955897513742 1106 72-73 0.5936300812708524 1106 74-75 0.7461678402391456 1106 76-77 0.7405165276649512 1106 78-79 0.7893615120399708 1106 80-81 0.9000689542945501 1106 82-83 0.7593518894470712 1106 84-85 0.8254229037559568 1106 86-87 0.8415359244298166 1106 88-89 0.907387404570926 1106 90-91 1.0701007704301162 1106 92-93 1.357685239584029 1106 94-95 1.445305720282004 1106 96-97 1.3678276213851923 1106 98-99 1.4617380749059947 1106 100-101 1.4232710510027644 1107 1 0.27449352961697926 1107 2 0.36942675446895024 1107 3 0.38212096367528403 1107 4 0.009666948470076875 1107 5 0.4332635479361997 1107 6 0.356478601151899 1107 7 0.2280888330702382 1107 8 0.29176599707728457 1107 9 0.16331305731878842 1107 10-11 0.35108307574649444 1107 12-13 0.46906163669094525 1107 14-15 0.49529279312812946 1107 16-17 0.5079416316577365 1107 18-19 0.6080729672226397 1107 20-21 0.8248102670652102 1107 22-23 0.6905662669234403 1107 24-25 0.6381001961387369 1107 26-27 0.7167664300079437 1107 28-29 0.8032015741464065 1107 30-31 0.577503109356428 1107 32-33 0.7778248046520986 1107 34-35 0.7416927040235208 1107 36-37 0.7453860665072227 1107 38-39 0.8250562327311997 1107 40-41 0.9553930658025394 1107 42-43 0.9211696952561326 1107 44-45 0.9499530273269272 1107 46-47 0.886050406539745 1107 48-49 0.9155059058790442 1107 50-51 0.859862478593918 1107 52-53 0.9998722844899177 1107 54-55 1.1018417979922788 1107 56-57 0.9788720691215431 1107 58-59 1.0382508022322483 1107 60-61 1.0882347706264355 1107 62-63 1.1232701808069336 1107 64-65 1.2317428881288706 1107 66-67 1.045057641074166 1107 68-69 0.9248441432743917 1107 70-71 0.9803511160547131 1107 72-73 0.9585367310882376 1107 74-75 1.0982009710453937 1107 76-77 1.0511788843354495 1107 78-79 0.9677333896202107 1107 80-81 0.7846659070784163 1107 82-83 1.0194221558152137 1107 84-85 1.0750432608568445 1107 86-87 1.359393258062287 1107 88-89 1.09975532318159 1107 90-91 1.312373988168865 1107 92-93 1.3004610358420834 1107 94-95 1.5604516023584338 1107 96-97 1.571332240437389 1107 98-99 1.6554002133295178 1107 100-101 1.911346830014729 1108 1 0.07686004398122748 1108 2 0.1015000766307601 1108 3 0.11041786900265294 1108 4 0.0478545697795596 1108 5 -4.8640685529477423E-4 1108 6 0.15098787793642998 1108 7 0.13558470402708878 1108 8 0.14464547723979848 1108 9 0.03500680890108043 1108 10-11 0.06392093394852338 1108 12-13 0.19145506518314903 1108 14-15 0.27098327764271346 1108 16-17 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84-85 0.1744278054272499 2314 86-87 0.2524495250912473 2314 88-89 0.43778957784205375 2314 90-91 0.32710779812751056 2314 92-93 1.388311901635575E-4 2314 94-95 0.02961052943178899 2314 96-97 0.1603156624883617 2314 98-99 -0.11172006403375079 2314 100-101 -0.3894319549619176 2315 1 -0.3857941252571031 2315 2 -0.497536986752543 2315 3 -0.2039095399080395 2315 4 -0.4416386462509081 2315 5 -0.23312242622910162 2315 6 -0.4716701618782011 2315 7 -0.3214296694102927 2315 8 -0.087562948063308 2315 9 -0.4339671222445034 2315 10-11 -0.41710212736349916 2315 12-13 -0.7140355303625512 2315 14-15 -0.5070940920391678 2315 16-17 -0.6879822337477179 2315 18-19 -0.809712848416325 2315 20-21 -0.8867107616478336 2315 22-23 -0.5224373875730635 2315 24-25 -0.5747581175785896 2315 26-27 -0.7545909479844752 2315 28-29 -0.6318378401752014 2315 30-31 -0.7385044543575248 2315 32-33 -0.9132518708498907 2315 34-35 -0.8420348224712058 2315 36-37 -1.0050660631846995 2315 38-39 -1.0709486938717063 2315 40-41 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3 -2.170015314310483 2316 4 -1.6222272200767662 2316 5 -1.7003630729449597 2316 6 -1.2964870225309468 2316 7 -1.269783566701662 2316 8 -0.9644293020574182 2316 9 -1.136935808374318 2316 10-11 -2.410590057915762 2316 12-13 -1.8264067142871667 2316 14-15 -2.5351552890874203 2316 16-17 -2.2327646448404224 2316 18-19 -2.498706066075151 2316 20-21 -2.0882238799112116 2316 22-23 -1.9599091502287678 2316 24-25 -1.9093733367003765 2316 26-27 -2.715026394726145 2316 28-29 -2.8048729244031634 2316 30-31 -2.4484154932766558 2316 32-33 -2.576751472837799 2316 34-35 -2.2429143485140983 2316 36-37 -1.8772376225315242 2316 38-39 -2.3826056074492996 2316 40-41 -2.6140429419330573 2316 42-43 -1.6418223026116223 2316 44-45 -1.6779912118816611 2316 46-47 -1.6157866437664623 2316 48-49 -1.8871665335626062 2316 50-51 -1.6972836548452008 2316 52-53 -1.5222281047695034 2316 54-55 -1.4744697830951665 2316 56-57 -1.8475991207229967 2316 58-59 -1.9473797608302519 2316 60-61 -1.895669538500087 2316 62-63 -2.094367981491935 2316 64-65 -2.4599100387548205 2316 66-67 -2.10471348306735 2316 68-69 -2.2886899045276614 2316 70-71 -2.040903191832129 2316 72-73 -2.1210925427074088 2316 74-75 -2.738275983309599 2316 76-77 -2.8067607990503625 2316 78-79 -3.204086212194685 2316 80-81 -3.1619968752586267 2316 82-83 -3.8307734036522234 2316 84-85 -3.5052965043478252 2316 86-87 -3.4814574411170476 2316 88-89 -3.378372078697762 2316 90-91 -3.7825606807126952 2316 92-93 -3.956555551071858 2316 94-95 -4.159397838546038 2316 96-97 -4.503318944696371 2316 98-99 -4.8944364588743845 2316 100-101 -5.500537514070761 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1664.0 3 632.0 4 611.0 5 661.0 6 679.0 7 810.0 8 996.0 9 1294.0 10 1548.0 11 1941.0 12 2438.0 13 2995.0 14 3321.0 15 4336.0 16 5616.0 17 7588.0 18 6769.0 19 4293.0 20 4265.0 21 4615.0 22 5130.0 23 5618.0 24 6348.0 25 7461.0 26 8526.0 27 10108.0 28 12347.0 29 15018.0 30 18756.0 31 23564.0 32 30579.0 33 41131.0 34 57442.0 35 85136.0 36 133564.0 37 200027.0 38 177916.0 39 30160.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.030725517628824 18.78524008362008 14.57733506911621 22.606699329634893 2 15.216522376031405 20.168460306931784 40.630327176404755 23.984690140632058 3 19.340488875637885 24.82845710779548 29.703170302771042 26.12788371379559 4 12.847124673135522 16.602662459749745 37.69800963845007 32.85220322866466 5 13.992106312895725 36.94822835262793 34.25419142677692 14.805473907699426 6 31.3513478445624 35.85663358371709 18.093479245756324 14.698539325964186 7 28.306578414156427 32.38384438741051 21.229231392678642 18.080345805754426 8 26.0846723849983 34.200919905523214 20.22613893708214 19.488268772396346 9 26.27963957498641 16.908675685578135 20.737515955302456 36.074168784133 10-11 24.667185465000028 26.413158022729853 27.74349745734775 21.17615905492237 12-13 25.861602170683557 23.91442855004412 28.07243753907776 22.151531740194557 14-15 22.930648406919165 25.192367131589926 26.462422485772397 25.414561975718513 16-17 22.044916587108755 28.608809019408888 26.892951520190962 22.453322873291395 18-19 22.037231961648445 27.17942926554088 29.17881543313009 21.60452333968059 20-21 22.974447861577996 26.08855394736558 29.02084715615484 21.91615103490159 22-23 22.571597881814405 26.059430414859065 29.10652347360398 22.262448229722555 24-25 22.518318420813873 26.29152427406777 29.07665870276202 22.113498602356334 26-27 22.30394499589316 26.25299102651544 29.15067537392219 22.29238860366921 28-29 21.918518402510948 26.477711935480908 29.304364402820926 22.299405259187214 30-31 22.162231052288803 26.1439694473456 29.19588671065379 22.49791278971181 32-33 22.094341324269344 26.340386764111045 29.139833549618384 22.42543836200123 34-35 22.458051140588825 26.3973637468752 28.787035181903946 22.357549930632022 36-37 22.443240884600286 26.05737664636672 29.05063321900775 22.448749250025244 38-39 22.141200816082307 26.37643686540582 29.107547681304524 22.37481463720735 40-41 22.285988934582264 26.37274094555588 29.00581352961984 22.33545659024202 42-43 22.16313770640354 26.361222460852385 28.706758333769848 22.768881498974224 44-45 22.28606359608859 26.363097333854626 28.629310102753415 22.721528967303374 46-47 22.59039495241973 26.632476841676667 28.247484364014174 22.52964384188943 48-49 22.534499377647695 26.697698781485492 28.017733665614564 22.750068175252245 50-51 22.26971326987473 26.787830939550528 28.197586038748014 22.744869751826723 52-53 22.497938428820845 26.983010061331402 27.786577295013398 22.732474214834347 54-55 22.408030187733612 26.71373553760964 27.94914488070671 22.92908939395003 56-57 22.381411424791068 27.03676401096305 27.727597985607627 22.854226578638258 58-59 22.6805727088971 26.733039522918993 27.818816791151775 22.767570977032133 60-61 22.502860716196974 26.546528286664557 28.43133390106647 22.519277096072003 62-63 22.319725481131687 26.68577008072687 28.33730091009442 22.657203528047024 64-65 22.28533195521549 26.90164871927383 28.056038418814982 22.7569809066957 66-67 22.19485044513495 26.964327090875802 27.709900451589313 23.13092201239994 68-69 22.016304612076652 26.80698645604292 28.250792810411614 22.92591612146882 70-71 22.284664228780883 26.750879627228247 27.97084911043104 22.993607033559833 72-73 22.275408228398376 26.613823873436505 27.991523840279253 23.11924405788586 74-75 22.108639449646823 27.153759617975915 27.77014178464051 22.967459147736754 76-77 22.267591552878766 26.819053606564726 27.774456094640428 23.138898745916084 78-79 22.272605250265613 26.7808269862421 27.82637534413649 23.1201924193558 80-81 22.223831389093487 27.05255689110401 27.639152254866094 23.084459464936405 82-83 22.21059687714543 26.949650492432692 27.862027019654633 22.97772561076724 84-85 22.139021895397658 26.835498822113802 27.67352083740634 23.351958445082204 86-87 22.077354548342 26.741347011885907 27.92145707698723 23.259841362784858 88-89 22.181480865188668 26.661289311777814 27.630887987500618 23.5263418355329 90-91 22.125581420475726 26.600074551475632 27.909371336578303 23.36497269147034 92-93 22.320243447989355 26.609701508260343 27.923912239009486 23.14614280474082 94-95 21.943557076158253 26.530874565579595 27.96557581125564 23.559992547006516 96-97 21.74569379902739 26.586429714893097 28.158438814463395 23.509437671616123 98-99 21.783879095376186 26.817826257946574 27.717393653112293 23.68090099356495 100-101 21.655734967404854 26.718452856067433 27.873212103787825 23.752600072739888 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 101.0 1 86.5 2 89.0 3 101.0 4 93.5 5 101.0 6 164.5 7 350.0 8 630.0 9 813.0 10 786.0 11 742.0 12 798.5 13 859.0 14 906.5 15 978.0 16 1038.5 17 1082.5 18 1133.0 19 1247.5 20 1398.0 21 1554.5 22 1842.5 23 2232.0 24 2670.0 25 3358.5 26 4369.5 27 5483.0 28 6546.0 29 7824.5 30 9482.0 31 11413.0 32 13231.0 33 15169.0 34 17616.0 35 19703.0 36 22103.0 37 24546.0 38 25963.0 39 26849.5 40 27549.0 41 29043.0 42 30133.0 43 31303.0 44 33401.0 45 34701.5 46 35220.0 47 36618.0 48 39962.0 49 39666.0 50 36076.0 51 34224.0 52 32892.0 53 31969.5 54 31051.5 55 29016.0 56 26565.0 57 23944.0 58 21526.0 59 19002.5 60 16056.5 61 12857.5 62 9885.5 63 7649.5 64 5732.0 65 4254.5 66 3079.5 67 2361.5 68 1654.5 69 1013.0 70 709.5 71 485.0 72 322.0 73 203.5 74 129.5 75 92.0 76 59.0 77 28.0 78 18.0 79 13.0 80 13.0 81 10.0 82 3.5 83 2.0 84 5.0 85 6.0 86 4.5 87 2.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03326410511457216 2 0.02646008361386422 3 0.02235607064518324 4 0.05000415801313932 5 0.09504030032734903 6 0.1162083672184404 7 0.059184187022030994 8 0.08942428258073296 9 0.0736562327536955 10-11 0.07646424162700355 12-13 0.07392623360689819 14-15 0.06577220784017677 16-17 0.053622169446055454 18-19 0.06361220101455521 20-21 0.056268177807441866 22-23 0.03450610903930457 24-25 0.008532026961205199 26-27 0.0030780097265107357 28-29 0.006210019623662012 30-31 0.004968015698929609 32-33 0.006642020988786326 34-35 0.006858021671348482 36-37 0.006318019964943088 38-39 0.0038880122861188245 40-41 0.007074022353910638 42-43 0.003942012456759363 44-45 0.0018360058017783338 46-47 0.0015120047779350982 48-49 5.4000170640539227E-5 50-51 0.0018900059724188728 52-53 0.0055620175759755405 54-55 0.01258203975924564 56-57 0.009234029179532208 58-59 0.004590014504445834 60-61 0.001998006313699951 62-63 0.009180029008891668 64-65 0.002430007678824265 66-67 0.0207900656966076 68-69 0.04514414265549079 70-71 0.057348181220252654 72-73 0.0034560109209945105 74-75 0.012690040100526719 76-77 0.02025006399020221 78-79 0.025542080712975052 80-81 0.07219822814640095 82-83 0.028458089927564174 84-85 0.012096038223480786 86-87 0.031212098630231673 88-89 0.047196149139831285 90-91 0.04190413241705843 92-93 0.04174213190513682 94-95 0.013014041124369952 96-97 0.017820056311377945 98-99 0.039636125250155786 100-101 0.07651824179764408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 925923.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.321401731635106 #Duplication Level Percentage of deduplicated Percentage of total 1 81.77169517009938 51.778983601426695 2 10.609587285902759 13.436278774749939 3 3.120926857779894 5.92864390009591 4 1.3930578185408131 3.528414950528723 5 0.7728729125662638 2.446969809205364 6 0.48608964411540445 1.8467926579571445 7 0.3364484426700488 1.4913070900205214 8 0.24152015839049168 1.2234715980585973 9 0.1766242243412488 1.0065684118545593 >10 1.0119067497130974 11.531156703180171 >50 0.059626987786815425 2.5391311317200445 >100 0.01793559618765889 1.8569351354431194 >500 8.540759530161292E-4 0.3580181168303439 >1k 8.540759530161292E-4 1.027328118928884 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3133 0.33836506923361875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2301 0.24850878528776152 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1526 0.1648085207949257 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1388 0.1499044736981369 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1150 0.12420039247324022 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0800034128107845E-4 0.0 18-19 0.0 0.0 0.0 1.6200051192161768E-4 0.0 20-21 0.0 0.0 0.0 2.7000085320269616E-4 0.0 22-23 0.0 0.0 0.0 3.2400102384323536E-4 0.0 24-25 0.0 0.0 0.0 4.86001535764853E-4 0.0 26-27 0.0 0.0 0.0 9.72003071529706E-4 0.0 28-29 0.0 0.0 0.0 0.002592008190745883 0.0 30-31 0.0 0.0 0.0 0.005724018087897157 0.0 32-33 0.0 0.0 0.0 0.009180029008891668 0.0 34-35 0.0 0.0 0.0 0.012312038906042943 0.0 36-37 0.0 0.0 0.0 0.01576804982703745 0.0 38-39 0.0 0.0 0.0 0.019656062113156278 0.0 40-41 0.0 0.0 0.0 0.0265680839551453 0.0 42-43 0.0 0.0 0.0 0.03569411279339643 0.0 44-45 0.0 0.0 0.0 0.0556201757597554 0.0 46-47 0.0 0.0 0.0 0.0972003071529706 0.0 48-49 0.0 0.0 0.0 0.12214838598889974 0.0 50-51 0.0 0.0 0.0 0.15179447967055576 0.0 52-53 0.0 0.0 0.0 0.18468058359064415 0.0 54-55 0.0 0.0 0.0 0.2248027103765648 0.0 56-57 0.0 0.0 0.0 0.27210685985767713 0.0 58-59 0.0 0.0 0.0 0.34246908220229977 0.0 60-61 0.0 0.0 0.0 0.4271953499373058 0.0 62-63 0.0 0.0 0.0 0.530065675007533 0.0 64-65 0.0 0.0 0.0 0.6043159096382744 0.0 66-67 1.0800034128107845E-4 0.0 0.0 0.7257622934088472 0.0 68-69 1.0800034128107845E-4 0.0 0.0 0.8820927874132083 0.0 70-71 1.0800034128107845E-4 0.0 0.0 0.9880951223805867 0.0 72-73 1.0800034128107845E-4 0.0 0.0 1.178175723035285 0.0 74-75 1.0800034128107845E-4 0.0 0.0 1.3319682090195406 0.0 76-77 1.0800034128107845E-4 0.0 0.0 1.4495265805039943 0.0 78-79 1.0800034128107845E-4 0.0 0.0 1.5981350501067584 0.0 80-81 1.0800034128107845E-4 0.0 0.0 1.7356734847282118 0.0 82-83 1.0800034128107845E-4 0.0 0.0 1.9140900485245533 0.0 84-85 1.0800034128107845E-4 0.0 0.0 2.225131031414059 0.0 86-87 1.0800034128107845E-4 0.0 0.0 2.6106382496168687 0.0 88-89 1.0800034128107845E-4 0.0 0.0 3.0806557348721224 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4050 0.0 26.166927 1 GTATCAA 5535 0.0 25.328373 1 TACATGG 4185 0.0 25.09708 2 ACATGGG 4180 0.0 23.876429 3 CCCTATA 240 2.2737368E-10 23.762733 2 CCTATAC 240 2.2737368E-10 23.762733 3 CACCGTA 120 1.7073865E-4 23.756311 7 GGTATCA 4130 0.0 23.47378 1 CATGGGG 1975 0.0 21.89781 4 CAACGCA 6420 0.0 21.467888 5 TGCACCG 200 5.356069E-7 21.386457 5 TCAACGC 6445 0.0 21.384617 4 TATCAAC 6520 0.0 21.357304 2 ATCAACG 6455 0.0 21.351486 3 CGATTCG 90 5.1838833E-6 21.105312 38-39 AACGCAG 6575 0.0 21.032946 6 GAGTACT 2595 0.0 20.96301 12-13 GTACTTT 2720 0.0 20.088598 14-15 TATACAG 515 0.0 19.379316 5 TACCTGG 520 0.0 19.192976 2 >>END_MODULE