FastQCFastQC Report
Thu 26 May 2016
SRR936936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925923
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40910.4418293961808919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33530.362125144315456No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23000.24840078494648044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.22518071157104858No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.10119631978037051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71350.025.6993561
GTACATG41150.023.0881461
GAGTACT41300.022.94925912-13
ATTCGCG1053.452078E-822.61722280-81
GTACTTT42750.022.22462814-15
TACATGG42000.022.0529822
TCAACGC83800.021.9355724
GGACCGC1302.9392936E-421.9248586
ATCAACG84050.021.8703253
AGTACTT43200.021.71996912-13
CAACGCA85050.021.613185
CTCGCGC1105.6455974E-821.59033260-61
TATCAAC85550.021.486862
GGTATCA52900.021.4620481
AACGCAG86050.021.3643176
ACATGGG41050.021.290543
CCCGCGA1301.4901161E-820.09778820-21
AGAGTAC68400.019.86594410-11
TCGCGCT1201.4147554E-719.7911460-61
CTTACGC1200.004763548319.791142
Produced by FastQC (version 0.11.5)