##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936936_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 925923 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40285855303303 33.0 31.0 34.0 31.0 34.0 2 32.52799638846859 34.0 31.0 34.0 31.0 34.0 3 32.6559292727365 34.0 31.0 34.0 31.0 34.0 4 36.25636581011596 37.0 35.0 37.0 35.0 37.0 5 36.13323786103164 37.0 35.0 37.0 35.0 37.0 6 36.22458562969059 37.0 36.0 37.0 35.0 37.0 7 36.19039704165465 37.0 36.0 37.0 35.0 37.0 8 36.152293441247274 37.0 36.0 37.0 35.0 37.0 9 37.900809246557216 39.0 38.0 39.0 35.0 39.0 10-11 37.81796272476221 39.0 38.0 39.0 35.0 39.0 12-13 37.73207437335502 39.0 37.0 39.0 35.0 39.0 14-15 39.00190026600484 40.0 38.0 41.0 36.0 41.0 16-17 38.99609308765416 40.0 38.0 41.0 36.0 41.0 18-19 39.1031964861009 40.0 38.5 41.0 36.0 41.0 20-21 39.10151006077179 40.0 39.0 41.0 36.0 41.0 22-23 39.012415179231965 40.0 38.0 41.0 35.5 41.0 24-25 38.92398881980467 40.0 38.0 41.0 35.0 41.0 26-27 38.73655206750453 40.0 38.0 41.0 35.0 41.0 28-29 38.58943400261145 40.0 38.0 41.0 35.0 41.0 30-31 38.60672431724885 40.0 38.0 41.0 35.0 41.0 32-33 38.486972458832966 40.0 38.0 41.0 35.0 41.0 34-35 38.37627426902669 40.0 38.0 41.0 34.0 41.0 36-37 38.25604180909212 40.0 38.0 41.0 34.0 41.0 38-39 38.09556626198939 40.0 38.0 41.0 34.0 41.0 40-41 37.72761395926011 40.0 37.5 41.0 33.0 41.0 42-43 37.559733908759156 40.0 37.0 41.0 33.0 41.0 44-45 37.33756532670643 40.0 37.0 41.0 33.0 41.0 46-47 36.94951254045962 40.0 37.0 41.0 31.5 41.0 48-49 36.56825945569988 39.0 36.0 41.0 31.0 41.0 50-51 36.281819330549084 39.0 36.0 40.5 30.0 41.0 52-53 36.03469132962461 39.0 35.5 40.0 30.0 41.0 54-55 35.65129767810066 39.0 35.0 40.0 28.0 41.0 56-57 35.44826513651783 38.0 35.0 40.0 28.0 41.0 58-59 35.12703216142163 38.0 34.5 40.0 28.0 41.0 60-61 34.726159194663055 38.0 34.0 40.0 27.0 41.0 62-63 34.35835377239792 37.0 34.0 40.0 26.0 41.0 64-65 34.02896407152647 37.0 34.0 39.0 26.0 40.0 66-67 33.70279872084396 36.0 33.0 39.0 26.0 40.0 68-69 33.252440537712104 36.0 33.0 39.0 25.0 40.0 70-71 32.8037309797899 35.0 32.0 38.0 24.0 40.0 72-73 32.392290179636966 35.0 32.0 37.5 23.5 39.5 74-75 31.92092809013276 35.0 31.5 37.0 22.0 39.0 76-77 30.500189000597242 33.5 29.5 35.5 20.5 37.5 78-79 31.16024280636727 34.0 31.0 36.0 21.0 38.0 80-81 31.02244679093186 34.0 31.0 35.5 20.0 37.0 82-83 30.688112294434852 34.0 31.0 35.0 19.0 37.0 84-85 30.270358334332336 34.0 30.5 35.0 18.0 36.0 86-87 29.917609779646902 34.0 30.0 35.0 14.5 36.0 88-89 29.560588191458685 34.0 30.0 35.0 8.5 35.5 90-91 29.147120764901615 34.0 29.0 35.0 4.5 35.0 92-93 28.697685984687713 34.0 29.0 35.0 2.0 35.0 94-95 28.176229016883692 33.0 29.0 35.0 2.0 35.0 96-97 27.44713221293779 33.0 27.0 34.0 2.0 35.0 98-99 26.671963003403093 32.5 25.5 34.0 2.0 35.0 100-101 23.575235197743226 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.03968472329577821 1101 2 0.14902133187597144 1101 3 0.10788498459443474 1101 4 -0.008518227081552254 1101 5 -0.04318066144222854 1101 6 0.01695360353352271 1101 7 0.016304404040916154 1101 8 0.13674071432802037 1101 9 0.15302393953740534 1101 10-11 0.10092324593436786 1101 12-13 0.08836855065099769 1101 14-15 0.17526415606403134 1101 16-17 0.15772249480237122 1101 18-19 -0.059853547632577886 1101 20-21 -0.15209715488578723 1101 22-23 -0.050782852361138 1101 24-25 -0.1009693100440785 1101 26-27 -0.02081272153336755 1101 28-29 0.022215132584030073 1101 30-31 0.13212435613651508 1101 32-33 0.02803919828754431 1101 34-35 -0.13324469639760395 1101 36-37 0.0022109015368201312 1101 38-39 -0.0363431064418549 1101 40-41 0.11608374764032448 1101 42-43 0.11782748031155421 1101 44-45 -0.07097995414689962 1101 46-47 -0.06453859168085074 1101 48-49 0.07741126807399468 1101 50-51 0.05652716206242303 1101 52-53 0.07207730709392024 1101 54-55 -0.02181793596310655 1101 56-57 0.06463591667222346 1101 58-59 0.09675976027394739 1101 60-61 -0.04180503953418935 1101 62-63 -0.003338815698676001 1101 64-65 0.14584354207663353 1101 66-67 -0.12200821076391577 1101 68-69 -0.3875219317711043 1101 70-71 -0.49499328762197337 1101 72-73 -0.5859161260384731 1101 74-75 -0.435179040317589 1101 76-77 -0.20592991640900138 1101 78-79 -0.2582769442038533 1101 80-81 -0.412923839375388 1101 82-83 -0.25691927718207097 1101 84-85 -0.4882683587771268 1101 86-87 -0.7741151430409623 1101 88-89 -0.6575551879503791 1101 90-91 -0.20483177840491962 1101 92-93 -0.4589785751941342 1101 94-95 -0.7234949115662523 1101 96-97 -0.8463848031898813 1101 98-99 -0.7985882533509567 1101 100-101 -0.6467215867456666 1102 1 -0.22894414137193309 1102 2 -0.12234301775717427 1102 3 0.027813734753472374 1102 4 -0.029511483793029925 1102 5 -0.21285494788354953 1102 6 -0.059088637443615255 1102 7 -0.049241208580482976 1102 8 0.02856182633446025 1102 9 0.08263291362452208 1102 10-11 0.1498969089395885 1102 12-13 0.17664095406081515 1102 14-15 0.0309885294471286 1102 16-17 -0.01810663281118252 1102 18-19 -0.013933449239935669 1102 20-21 -0.03488480491335366 1102 22-23 -0.19250854047344745 1102 24-25 -0.18615103954914503 1102 26-27 -0.02457666402456482 1102 28-29 -0.2112712324017636 1102 30-31 -0.2700343444020987 1102 32-33 -0.22709778801382896 1102 34-35 -0.03176668630387525 1102 36-37 -0.19803614032246486 1102 38-39 -0.10777319253541151 1102 40-41 -0.08495849333680638 1102 42-43 -0.1578150405756773 1102 44-45 -0.15795353233088605 1102 46-47 -0.34845118339382 1102 48-49 -0.26925313669296 1102 50-51 -0.15231863292463288 1102 52-53 -0.22530595767557315 1102 54-55 -0.09601531498680771 1102 56-57 -0.15723523986537913 1102 58-59 -0.32839079742570476 1102 60-61 -0.24527528848452107 1102 62-63 -0.3939830330106915 1102 64-65 -0.3272522211024693 1102 66-67 -0.30645199548761326 1102 68-69 -0.26974233976126527 1102 70-71 -0.3833791394392705 1102 72-73 -0.7387639991458954 1102 74-75 -0.4597931036790541 1102 76-77 -0.5091287373937767 1102 78-79 -0.7285205932135632 1102 80-81 -0.4420864416910071 1102 82-83 -0.2497094718468169 1102 84-85 -0.397843616421639 1102 86-87 -0.29940777631633253 1102 88-89 -0.2112523337156773 1102 90-91 -0.44897911289747583 1102 92-93 -0.57531090480947 1102 94-95 -0.5392949879053681 1102 96-97 -0.13085403428534903 1102 98-99 -0.18502851799322428 1102 100-101 -0.15996960404919847 1103 1 0.05757383007572514 1103 2 0.16189505100713575 1103 3 0.21782857707295733 1103 4 0.07522122694495437 1103 5 -0.028890518445777502 1103 6 -0.004633646394907487 1103 7 0.0024442362555632258 1103 8 0.06076991463351078 1103 9 0.14704526488254288 1103 10-11 0.06615256406732328 1103 12-13 0.048481223160735 1103 14-15 0.09928296332230246 1103 16-17 0.09919805807583515 1103 18-19 0.10179456398044096 1103 20-21 0.0019073159061875344 1103 22-23 0.0011250735548458124 1103 24-25 0.16298647377578845 1103 26-27 0.09506084675013682 1103 28-29 0.025584261551649945 1103 30-31 0.007881712335347402 1103 32-33 0.13285842459347208 1103 34-35 0.09393781382618016 1103 36-37 0.18768373767468916 1103 38-39 -0.012726242861582193 1103 40-41 0.17136288113338338 1103 42-43 -0.053619790117913624 1103 44-45 -0.1006078593005455 1103 46-47 0.06637698233011236 1103 48-49 0.26735281757638063 1103 50-51 0.12810110889040516 1103 52-53 0.12302313378064866 1103 54-55 0.26970377244006016 1103 56-57 0.11452967003511816 1103 58-59 0.17315400240700285 1103 60-61 0.14924797213334529 1103 62-63 0.21417845754582032 1103 64-65 0.08190551552031877 1103 66-67 0.21925046446107643 1103 68-69 0.34472139856507766 1103 70-71 0.24050959926960047 1103 72-73 0.2737652647056592 1103 74-75 -0.04018326286420759 1103 76-77 0.2638239405992948 1103 78-79 0.4938215605038998 1103 80-81 0.36974061723453033 1103 82-83 0.561896354911184 1103 84-85 0.3743031899294458 1103 86-87 0.08972940547912955 1103 88-89 0.1880819296003331 1103 90-91 -0.21737262308053218 1103 92-93 -0.22536751984254622 1103 94-95 -0.06084312853506546 1103 96-97 0.1314967136961478 1103 98-99 -0.3467377564445222 1103 100-101 0.2149870420966451 1104 1 -0.013917249110704688 1104 2 0.11697177093812883 1104 3 -0.009016080248841263 1104 4 -0.05367355136605312 1104 5 -0.07580537131229903 1104 6 -0.032316352567072215 1104 7 -0.022550858776249072 1104 8 0.08177719267202122 1104 9 0.015137434684760365 1104 10-11 0.027026489256442687 1104 12-13 0.09085859117038098 1104 14-15 0.2641911080609063 1104 16-17 0.12813503468458975 1104 18-19 0.07536846394399532 1104 20-21 0.09460187185863589 1104 22-23 0.045878626153225355 1104 24-25 -0.011237217687693146 1104 26-27 0.06967646852882581 1104 28-29 0.23544682901442826 1104 30-31 0.1696351061061847 1104 32-33 0.15140932570620436 1104 34-35 0.18828604476765776 1104 36-37 0.07492396900233445 1104 38-39 0.06487424670095976 1104 40-41 0.13812510384696708 1104 42-43 0.15738259852373915 1104 44-45 0.2927581322971591 1104 46-47 0.12631647215113873 1104 48-49 0.03350573424174996 1104 50-51 0.31061964655589236 1104 52-53 0.16043558399132962 1104 54-55 0.30232959655257474 1104 56-57 0.24377402465029974 1104 58-59 -0.0026571859032884504 1104 60-61 0.10099476366129778 1104 62-63 0.05679593542674155 1104 64-65 0.3624004034534778 1104 66-67 0.0958243363962481 1104 68-69 0.18808085020520338 1104 70-71 0.05868398568144073 1104 72-73 0.08130365581380516 1104 74-75 0.0602626999576934 1104 76-77 0.0774437136939099 1104 78-79 0.1105816032316227 1104 80-81 -0.033604259642981305 1104 82-83 0.009617958259642734 1104 84-85 0.14672979447689727 1104 86-87 0.03399261697201794 1104 88-89 -0.0421829698244629 1104 90-91 0.03779861480886737 1104 92-93 0.20823711792165867 1104 94-95 0.36904307620297416 1104 96-97 0.4225310547019916 1104 98-99 0.12835045512622045 1104 100-101 0.8169605521541676 1105 1 0.09804159684051683 1105 2 0.19821899479590144 1105 3 0.06254279462591228 1105 4 0.09165788127828023 1105 5 -0.02384735174759811 1105 6 0.16133803192991536 1105 7 0.23266002473424408 1105 8 0.15568713720082883 1105 9 0.29642184593600973 1105 10-11 0.22188171666768142 1105 12-13 0.1701762025001443 1105 14-15 0.40592001196247907 1105 16-17 0.1295853430300582 1105 18-19 0.25388448034777156 1105 20-21 0.28875880265909615 1105 22-23 0.2536781654427429 1105 24-25 0.23101385919723327 1105 26-27 0.1546506955991802 1105 28-29 0.1576239443056764 1105 30-31 0.10197161069780236 1105 32-33 0.24423525771525334 1105 34-35 0.14975474021323265 1105 36-37 0.45162761646584215 1105 38-39 0.23461713719441235 1105 40-41 0.47907617143504666 1105 42-43 0.47930663294273756 1105 44-45 0.31907957599441517 1105 46-47 0.3331408753451157 1105 48-49 0.48862468225170375 1105 50-51 0.49639856750169287 1105 52-53 0.5389516003951087 1105 54-55 0.6365905487564447 1105 56-57 0.5600243991066876 1105 58-59 0.5475447623029339 1105 60-61 0.5675818906252559 1105 62-63 0.580230707705617 1105 64-65 0.6942973737138942 1105 66-67 0.6947102640667708 1105 68-69 0.5752140120633342 1105 70-71 0.5555328705761298 1105 72-73 0.44395098503586894 1105 74-75 0.45355554035146284 1105 76-77 0.49908109528821853 1105 78-79 0.6587795641228809 1105 80-81 0.5431928727957889 1105 82-83 0.6550072379604934 1105 84-85 0.3422657577853343 1105 86-87 0.36661474140516503 1105 88-89 0.4505804667038902 1105 90-91 0.54381470868638 1105 92-93 0.35722221197477566 1105 94-95 0.5788063164645258 1105 96-97 0.7984393051384266 1105 98-99 0.7576608355739154 1105 100-101 0.8902532892496247 1106 1 -0.0020174271689228362 1106 2 0.03343919243113902 1106 3 0.00755145782729727 1106 4 0.04219576136434 1106 5 -0.08203740905801027 1106 6 0.0621434240587746 1106 7 -0.011634638352902016 1106 8 0.13818937440118617 1106 9 0.15462332724824535 1106 10-11 0.18646159900046655 1106 12-13 0.23447429278783716 1106 14-15 0.23656299286535187 1106 16-17 0.21900290933686506 1106 18-19 0.08346122108005005 1106 20-21 0.027272920821353352 1106 22-23 0.02093126420324154 1106 24-25 0.1848266198072821 1106 26-27 0.19172538953112905 1106 28-29 0.1818218841773529 1106 30-31 0.12575067050244826 1106 32-33 0.1491094984951289 1106 34-35 0.06165283811693456 1106 36-37 0.07607498037742033 1106 38-39 0.10746990085085883 1106 40-41 0.18340206012719307 1106 42-43 -0.023892087569052478 1106 44-45 -0.11820836597165396 1106 46-47 -0.1745969818929396 1106 48-49 -0.2763188641392631 1106 50-51 -0.1435759188942285 1106 52-53 -0.3268258981292931 1106 54-55 -0.20847904245469095 1106 56-57 -0.04505737770639229 1106 58-59 0.14964297063864507 1106 60-61 -0.23232100535920353 1106 62-63 -0.04867392091912848 1106 64-65 -0.0786334539550424 1106 66-67 -0.11931269817690549 1106 68-69 -0.10232640034313079 1106 70-71 -0.05996569190364198 1106 72-73 0.18791244806970298 1106 74-75 0.005715628697078756 1106 76-77 -0.11406602681532263 1106 78-79 -0.053567877852710666 1106 80-81 -0.009233775592473847 1106 82-83 0.001314429369639214 1106 84-85 -0.12574703709671198 1106 86-87 0.05564008364921946 1106 88-89 0.28732957658374403 1106 90-91 -0.06853463728951681 1106 92-93 0.1430812017122136 1106 94-95 0.42513635587733134 1106 96-97 0.6015580576374298 1106 98-99 0.6645609326779294 1106 100-101 0.9930883457032316 1107 1 0.21725057553550897 1107 2 0.3489754771350775 1107 3 0.187876874747694 1107 4 0.08838780954350511 1107 5 0.062840426054116 1107 6 0.1795740314416321 1107 7 0.14938736796252527 1107 8 0.21408743017168064 1107 9 0.2746271122307178 1107 10-11 0.15928145672785377 1107 12-13 0.19260139799355613 1107 14-15 0.34686699716313996 1107 16-17 0.28221669521980175 1107 18-19 0.26320210240101005 1107 20-21 0.3407503582050708 1107 22-23 0.1968123791247649 1107 24-25 0.25201082177883194 1107 26-27 0.2770744549609603 1107 28-29 0.23669878830587976 1107 30-31 0.32200277475429573 1107 32-33 0.2620698584347494 1107 34-35 0.304235111884104 1107 36-37 0.3912045320859292 1107 38-39 0.39172132136393856 1107 40-41 0.27652560164982987 1107 42-43 0.2858202079770251 1107 44-45 0.10143813497993648 1107 46-47 0.23792032142915076 1107 48-49 0.18782834725963937 1107 50-51 0.3730039831540921 1107 52-53 0.412658184340593 1107 54-55 0.5121159934187034 1107 56-57 0.4125767640564604 1107 58-59 0.36965041850738345 1107 60-61 0.37922532786272 1107 62-63 0.4866408812621188 1107 64-65 0.7204328152884614 1107 66-67 0.5223356922135096 1107 68-69 0.23181124176540635 1107 70-71 0.14310243320224458 1107 72-73 0.40827244874123636 1107 74-75 0.6567753474789875 1107 76-77 0.41971112623685514 1107 78-79 0.7588915823904756 1107 80-81 0.7005282741586747 1107 82-83 0.747143699188527 1107 84-85 0.7983739846654103 1107 86-87 1.019328671163965 1107 88-89 0.9956943878458979 1107 90-91 1.1678024153995743 1107 92-93 1.2206374766179344 1107 94-95 1.2458449412248704 1107 96-97 1.2458215961992245 1107 98-99 1.713725260439098 1107 100-101 2.087929628262856 1108 1 -0.02743591857533545 1108 2 0.1114741208800254 1108 3 0.024678704787838512 1108 4 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-2.3606585315566484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 5.0 5 12.0 6 16.0 7 75.0 8 160.0 9 379.0 10 633.0 11 1070.0 12 1709.0 13 2444.0 14 3052.0 15 4649.0 16 6367.0 17 9475.0 18 8705.0 19 4763.0 20 4046.0 21 3977.0 22 4150.0 23 4542.0 24 5166.0 25 6180.0 26 7593.0 27 9442.0 28 11746.0 29 15110.0 30 19025.0 31 24527.0 32 32107.0 33 43888.0 34 62992.0 35 95045.0 36 154289.0 37 228890.0 38 142597.0 39 7096.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83281968520013 19.404773092831505 14.85291083478894 21.909496387179427 2 15.859465706154586 20.935203235788073 39.6082709162486 23.597060141808736 3 19.940081151807014 25.11267184278538 29.766102650447824 25.181144354959773 4 13.418610402808874 17.265258558217045 37.13051733243477 32.185613706539314 5 14.140666850991032 37.33842817397916 33.604131745839744 14.916773229190067 6 31.7815844298068 36.40896705233588 17.30111467152236 14.508333846334953 7 28.45086881249872 33.05969528652074 20.706994888321763 17.782441012658776 8 26.638392177319282 34.41117673931849 19.547305769486233 19.40312531387599 9 26.827392774561165 17.12766612342495 20.231595931843145 35.813345170170734 10-11 24.968652900943166 26.84154081926899 27.130171731342674 21.05963454844517 12-13 26.4312470027089 24.139480426339006 27.79331328658639 21.635959284365697 14-15 23.309395797736645 25.499901715337376 26.381493538053448 24.809208948872534 16-17 22.265620359360337 28.744992834177356 26.767074583955687 22.222312222506623 18-19 22.015358786589978 27.16645453519447 29.539933007135073 21.278253671080478 20-21 22.99016486925643 26.012902905286712 29.69444835948853 21.30248386596833 22-23 22.69696341321168 26.106805427753084 29.451468180423973 21.744762978611266 24-25 22.696062998929172 26.200413534423312 29.57780932596265 21.525714140684865 26-27 22.57592972194202 26.31209756329367 29.624579608217537 21.48739310654677 28-29 22.036437233846083 26.590173459722806 29.793686502412193 21.57970280401892 30-31 22.293592447752133 26.235928905535346 29.589717503507313 21.880761143205213 32-33 22.438919024000388 26.453401877586945 29.41731084405012 21.69036825436254 34-35 22.722029801614173 26.36093930056819 29.267876486489698 21.649154411327938 36-37 22.72125653613389 26.01493340879059 29.48584123411496 21.77796882096056 38-39 22.440389525665232 26.308103741019007 29.541207806066684 21.710298927249084 40-41 22.538357733349965 26.393706161915453 29.266897895771944 21.801038208962638 42-43 22.45840096854706 26.59308603415187 28.887931285862862 22.060581711438207 44-45 22.72103318562327 26.34734580894749 28.843044648903298 22.088576356525937 46-47 22.995229082347603 26.940321679190216 28.226768439043227 21.83768079941896 48-49 23.08048293432607 27.01374736344167 27.95545633924203 21.950313362990226 50-51 22.79272234780533 27.18438108527567 27.946717960736706 22.07617860618229 52-53 23.053540889397933 27.38955966426851 27.454036181370174 22.102863264963386 54-55 22.712417771240155 27.1493957920907 27.743559669648555 22.394626767020583 56-57 22.799569293708643 27.4110858269766 27.619040933297768 22.170303946016993 58-59 23.07924093040134 27.085999591758707 27.59235919185505 22.242400285984903 60-61 22.88392603528887 27.019735371276195 28.25480261928651 21.841535974148425 62-63 22.73019610172558 27.060310123147456 28.25204053254997 21.957453242576996 64-65 22.697916239164854 27.27556964841531 27.905428319016075 22.121085793403765 66-67 22.508135125706996 27.293090246165182 27.793077826125927 22.405696802001895 68-69 22.537439728961907 27.13269969430454 27.901663369950906 22.42819720678265 70-71 22.76166854858176 27.24656651746347 27.677921976934254 22.313842957020515 72-73 22.706887585799844 27.072979886480415 27.768126710799372 22.45200581692037 74-75 22.722257893625535 27.568408541192014 27.406780182732387 22.302553382450068 76-77 22.70374826389264 27.388592127630623 27.365102028032602 22.542557580444143 78-79 22.81341800587524 27.2265422498704 27.336540953862105 22.623498790392258 80-81 22.86123307932863 27.557466965978563 27.18604516787153 22.395254786821276 82-83 22.789110319708183 27.55554595552003 27.239536786286113 22.415806938485673 84-85 22.641694873517384 27.510751724272005 27.163845261573087 22.68370814063752 86-87 22.61557295207629 27.405784196142317 27.224072935066168 22.754569916715223 88-89 22.54392227213178 27.339380082233767 27.080549996381887 23.036147649252563 90-91 22.622154612803776 27.244921598455136 27.209280850712247 22.92364293802884 92-93 22.804434062011637 27.19702394259566 27.2110639869622 22.787478008430504 94-95 22.56942531938401 27.21046998508515 27.140539764105654 23.079564931425185 96-97 22.444703646103335 27.149483756696043 27.314670813893212 23.091141783307414 98-99 22.467475381082906 27.44126344347315 26.848983814911538 23.242277360532405 100-101 22.01134031752889 27.869964359002054 26.729560427691972 23.38913489577708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 234.0 1 171.0 2 114.5 3 146.5 4 146.0 5 134.5 6 226.5 7 455.0 8 762.0 9 967.5 10 987.5 11 981.0 12 1059.0 13 1137.0 14 1246.0 15 1357.0 16 1421.5 17 1513.0 18 1602.5 19 1688.0 20 1823.0 21 2025.5 22 2296.0 23 2603.0 24 2985.0 25 3616.0 26 4560.5 27 5671.0 28 6760.5 29 8019.0 30 9620.5 31 11502.5 32 13203.5 33 14915.0 34 17190.0 35 19172.0 36 21592.0 37 23768.0 38 25245.0 39 26570.0 40 27199.0 41 28473.0 42 29718.5 43 30947.5 44 32729.0 45 33962.0 46 34653.0 47 36540.0 48 40118.5 49 39340.5 50 35661.0 51 33810.0 52 32421.5 53 31517.0 54 30468.5 55 28782.5 56 26452.0 57 23825.5 58 21805.5 59 19609.0 60 16546.5 61 13203.0 62 10153.0 63 7814.5 64 5889.0 65 4402.5 66 3248.5 67 2473.0 68 1702.5 69 1036.0 70 685.0 71 449.5 72 311.5 73 210.0 74 152.0 75 106.0 76 62.5 77 29.5 78 17.5 79 11.5 80 2.0 81 2.5 82 3.0 83 1.0 84 0.5 85 0.5 86 1.0 87 1.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020736065525967063 2 0.0030240095558701965 3 4.320013651243138E-4 4 0.0 5 0.0020520064843404903 6 0.0 7 4.320013651243138E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.009396029691453825 14-15 0.004428013992524216 16-17 0.0 18-19 3.7800119448377456E-4 20-21 8.100025596080884E-4 22-23 0.0016200051192161767 24-25 2.700008532026961E-4 26-27 1.0800034128107845E-4 28-29 2.160006825621569E-4 30-31 0.0 32-33 5.4000170640539227E-5 34-35 0.0 36-37 0.0013500042660134807 38-39 0.008532026961205199 40-41 1.6200051192161768E-4 42-43 0.0 44-45 0.0010800034128107844 46-47 5.4000170640539227E-5 48-49 0.0 50-51 0.002160006825621569 52-53 4.8600153576485307E-4 54-55 0.0 56-57 2.160006825621569E-4 58-59 0.0 60-61 0.002376007508183726 62-63 5.4000170640539227E-5 64-65 1.0800034128107845E-4 66-67 0.0 68-69 1.6200051192161768E-4 70-71 4.8600153576485307E-4 72-73 3.7800119448377456E-4 74-75 0.003618011432916128 76-77 1.0800034128107845E-4 78-79 3.2400102384323536E-4 80-81 0.0023220073375431866 82-83 5.4000170640539227E-5 84-85 0.0027000085320269614 86-87 3.7800119448377456E-4 88-89 0.0028080088733080395 90-91 0.0017820056311377943 92-93 0.0 94-95 0.0 96-97 3.2400102384323536E-4 98-99 9.720030715297061E-4 100-101 0.0024840078494648044 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 925923.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.17570344821106 #Duplication Level Percentage of deduplicated Percentage of total 1 80.41088298448 48.38781448482868 2 11.150200112107813 13.419422706688188 3 3.405611086318533 6.148051283707319 4 1.5384112124804417 3.7029990761450344 5 0.8604838631676455 2.589011088597363 6 0.5427186305280368 1.9595085219884623 7 0.3659406438278731 1.5414514963843462 8 0.27489052372777617 1.3233384509252868 9 0.2125172747346356 1.1509538851848087 >10 1.140923673556408 12.377027786692812 >50 0.06754558049972134 2.7411826949529585 >100 0.02752067292487042 2.7498485322302155 >500 0.0016295134473852358 0.6205926044267105 >1k 7.242281988378826E-4 1.2887973872478158 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4091 0.4418293961808919 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3353 0.362125144315456 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2300 0.24840078494648044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 0.22518071157104858 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 937 0.10119631978037051 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0800034128107845E-4 0.0 18-19 0.0 0.0 0.0 1.6200051192161768E-4 0.0 20-21 0.0 0.0 0.0 2.7000085320269616E-4 0.0 22-23 0.0 0.0 0.0 3.2400102384323536E-4 0.0 24-25 0.0 0.0 0.0 4.86001535764853E-4 0.0 26-27 0.0 0.0 0.0 8.640027302486276E-4 0.0 28-29 0.0 0.0 0.0 0.002430007678824265 0.0 30-31 0.0 0.0 0.0 0.0055620175759755405 0.0 32-33 0.0 0.0 0.0 0.009234029179532206 0.0 34-35 5.4000170640539227E-5 0.0 0.0 0.012420039247324021 0.0 36-37 1.0800034128107845E-4 0.0 0.0 0.01593005033895907 0.0 38-39 1.0800034128107845E-4 0.0 0.0 0.019818062625077897 0.0 40-41 1.0800034128107845E-4 0.0 0.0 0.026892084978988533 0.0 42-43 1.6200051192161768E-4 0.0 0.0 0.03618011432916128 0.0 44-45 2.160006825621569E-4 0.0 0.0 0.056106177295520254 0.0 46-47 2.160006825621569E-4 0.0 0.0 0.09757830834745437 0.0 48-49 2.160006825621569E-4 0.0 0.0 0.12274238786594566 0.0 50-51 2.160006825621569E-4 0.0 0.0 0.1522264810356801 0.0 52-53 2.160006825621569E-4 0.0 0.0 0.18468058359064415 0.0 54-55 2.160006825621569E-4 0.0 0.0 0.22447870935272157 0.0 56-57 2.160006825621569E-4 0.0 0.0 0.27183685900447446 0.0 58-59 2.160006825621569E-4 0.0 0.0 0.3418750803252538 0.0 60-61 2.160006825621569E-4 0.0 0.0 0.42573734533001123 0.0 62-63 2.160006825621569E-4 0.0 0.0 0.5290396717653627 0.0 64-65 2.160006825621569E-4 0.0 0.0 0.6031279058841825 0.0 66-67 2.160006825621569E-4 0.0 0.0 0.7246282898253958 0.0 68-69 2.160006825621569E-4 0.0 0.0 0.8810127840003974 0.0 70-71 2.160006825621569E-4 0.0 0.0 0.9871771194796976 0.0 72-73 2.160006825621569E-4 0.0 0.0 1.1776357213288795 0.0 74-75 2.160006825621569E-4 0.0 0.0 1.331806208507619 0.0 76-77 2.160006825621569E-4 0.0 0.0 1.448986578797589 0.0 78-79 2.160006825621569E-4 0.0 0.0 1.5980270497654772 0.0 80-81 2.160006825621569E-4 0.0 0.0 1.7370234889942253 0.0 82-83 2.160006825621569E-4 0.0 0.0 1.9165740563740181 0.0 84-85 2.160006825621569E-4 0.0 0.0 2.228425041823132 0.0 86-87 2.160006825621569E-4 0.0 0.0 2.614256261049785 0.0 88-89 2.7000085320269616E-4 0.0 0.0 3.083949745281195 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7135 0.0 25.699356 1 GTACATG 4115 0.0 23.088146 1 GAGTACT 4130 0.0 22.949259 12-13 ATTCGCG 105 3.452078E-8 22.617222 80-81 GTACTTT 4275 0.0 22.224628 14-15 TACATGG 4200 0.0 22.052982 2 TCAACGC 8380 0.0 21.935572 4 GGACCGC 130 2.9392936E-4 21.924858 6 ATCAACG 8405 0.0 21.870325 3 AGTACTT 4320 0.0 21.719969 12-13 CAACGCA 8505 0.0 21.61318 5 CTCGCGC 110 5.6455974E-8 21.590332 60-61 TATCAAC 8555 0.0 21.48686 2 GGTATCA 5290 0.0 21.462048 1 AACGCAG 8605 0.0 21.364317 6 ACATGGG 4105 0.0 21.29054 3 CCCGCGA 130 1.4901161E-8 20.097788 20-21 AGAGTAC 6840 0.0 19.865944 10-11 TCGCGCT 120 1.4147554E-7 19.79114 60-61 CTTACGC 120 0.0047635483 19.79114 2 >>END_MODULE