FastQCFastQC Report
Thu 26 May 2016
SRR936935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950807
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60710.6385102339381179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44250.46539413361491866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27650.2908055998746328No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21670.22791165820192744No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15190.1597590257539122No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11730.12336888558876827No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.10496346787518393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80750.029.2608991
GTACATG57950.027.7291771
GGTATCA55250.026.760971
TACATGG59400.025.9331462
ACATGGG58300.025.1978633
TCAACGC95450.024.804314
ATCAACG95750.024.7265933
CAACGCA96500.024.479995
TATCAAC96450.024.400552
AACGCAG97750.024.2155746
GAGTACT44100.024.1918112-13
GTACTTT46850.022.51999714-15
CATGGGA37400.022.4996324
AGTACTT46450.022.30290212-13
ACGCAGA108100.021.7640047
CGCAGAG108050.021.5090148
CATGGGG20600.021.2321684
AGAGTAC78900.021.10898410-11
GTATAGG2308.933421E-820.670261
GCAGAGT111100.020.490759
Produced by FastQC (version 0.11.5)